AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86849

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86849.t1 MTR_3g053920 45.000 340 94 4 42 354 1 274 4.66e-86 264
MS.gene86849.t1 MTR_2g061500 58.140 129 27 1 42 143 1 129 6.02e-47 158
MS.gene86849.t1 MTR_7g011710 82.353 85 15 0 69 153 1 85 2.01e-45 154
MS.gene86849.t1 MTR_4g103530 66.000 100 32 1 69 168 1 98 2.72e-41 145
MS.gene86849.t1 MTR_8g463110 57.500 120 51 0 303 422 1 120 2.58e-31 126
MS.gene86849.t1 MTR_6g033215 33.974 156 100 2 61 213 139 294 6.26e-27 109
MS.gene86849.t1 MTR_0010s0370 34.646 127 81 1 67 191 51 177 2.19e-21 91.7
MS.gene86849.t1 MTR_0010s0350 34.646 127 81 1 67 191 51 177 2.19e-21 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86849.t1 AT2G17920 26.042 192 125 3 41 230 43 219 1.30e-18 86.3
MS.gene86849.t1 AT2G41590 25.521 192 126 3 41 230 43 219 1.11e-16 81.6
MS.gene86849.t1 AT5G36228 29.412 170 116 2 73 240 62 229 1.25e-16 81.3
MS.gene86849.t1 AT3G57587 28.667 150 99 4 85 230 74 219 2.27e-16 80.5
MS.gene86849.t1 AT5G25200 24.479 192 128 3 41 230 43 219 1.36e-14 75.1
MS.gene86849.t1 AT5G18636 24.479 192 128 3 41 230 43 219 1.46e-14 75.1
MS.gene86849.t1 AT2G02103 25.000 192 127 3 41 230 43 219 1.72e-14 75.1
MS.gene86849.t1 AT3G31430 30.061 163 108 4 69 229 153 311 1.97e-14 74.3
MS.gene86849.t1 AT3G47920 27.703 148 103 2 85 230 3 148 4.33e-13 70.1

Find 134 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGACTCAGTGGCGAGGTTT+AGG 0.233947 6.2:-52400022 None:intergenic
CTGATGACGGAGTTCGTAAT+TGG 0.256041 6.2:+52400633 MS.gene86849:CDS
AGCAGCAGGCATGGAAGCTT+GGG 0.284414 6.2:-52400753 None:intergenic
TTCTGCTTCTGTAGGTCTTT+CGG 0.293163 6.2:+52400949 MS.gene86849:CDS
AGACTCAGTGGCGAGGTTTA+GGG 0.329947 6.2:-52400021 None:intergenic
CCCTCAAGTCTCAATATTGC+TGG 0.350835 6.2:+52399972 MS.gene86849:CDS
ATCTGAACCATATGGACATC+TGG 0.355218 6.2:+52400312 MS.gene86849:CDS
AGTGAGTCAAATCTTGTTCC+TGG 0.364702 6.2:+52399885 MS.gene86849:CDS
CCAAATGTTGGTCAGGGTAT+AGG 0.372172 6.2:+52400371 MS.gene86849:CDS
CCTATACCCTGACCAACATT+TGG 0.379164 6.2:-52400371 None:intergenic
CAAGCTTCCATGCCTGCTGC+TGG 0.379709 6.2:+52400755 MS.gene86849:CDS
GACATCTGGGTTCAGGTTCA+TGG 0.383774 6.2:+52400326 MS.gene86849:CDS
TCATTTGCTGCTGATGGGGA+TGG 0.384310 6.2:+52401121 MS.gene86849:CDS
CAGCAGCAGGCATGGAAGCT+TGG 0.385581 6.2:-52400754 None:intergenic
AATAGGTTCAAATGTTAGTC+AGG 0.388463 6.2:+52401156 MS.gene86849:CDS
GATGGTATTACGGTCATCAT+AGG 0.394603 6.2:-52400417 None:intergenic
TGCTAATAACAAATGGCTTA+AGG 0.406992 6.2:+52400697 MS.gene86849:CDS
CCATATGGACATCTGGGTTC+AGG 0.407479 6.2:+52400319 MS.gene86849:CDS
AAACCGATTGCTACTCGTAT+TGG 0.407524 6.2:+52400668 MS.gene86849:CDS
TAATCAACCAAATGTTGGTC+AGG 0.408363 6.2:+52400364 MS.gene86849:CDS
TTGCCCGGACTTGTTCGAAT+TGG 0.408985 6.2:+52400607 MS.gene86849:CDS
ATTTGGTTGATTACAGCAAA+AGG 0.418327 6.2:-52400354 None:intergenic
CATTTGCTGCTGATGGGGAT+GGG 0.418666 6.2:+52401122 MS.gene86849:CDS
ATTTGCTGCTGATGGGGATG+GGG 0.423209 6.2:+52401123 MS.gene86849:CDS
ATGGCTCGTGCTTGGAAGCC+AGG 0.426942 6.2:+52400128 MS.gene86849:CDS
AAGTGTTGTGGATGGTATTA+CGG 0.427130 6.2:-52400427 None:intergenic
GAGGGATCCATAGGAACGTT+TGG 0.432983 6.2:-52399954 None:intergenic
GTCATGGACCGTATCCAACC+TGG 0.437630 6.2:+52400269 MS.gene86849:CDS
TGCTGCTCCGTTTACAATTG+AGG 0.438984 6.2:+52401015 MS.gene86849:CDS
TGAAAGTTGCGGCCAGCAGC+AGG 0.439396 6.2:-52400767 None:intergenic
GCAGGTAAACCGAGCACCAC+TGG 0.442493 6.2:-52400995 None:intergenic
TGATGACGGAGTTCGTAATT+GGG 0.442501 6.2:+52400634 MS.gene86849:CDS
CCTTGTTCCAAACGTTCCTA+TGG 0.444317 6.2:+52399947 MS.gene86849:CDS
ACAATAGAGACTCGCTTGCC+TGG 0.445919 6.2:-52400146 None:intergenic
GTTCAGCTGCTAATAACAAA+TGG 0.448222 6.2:+52400690 MS.gene86849:CDS
ACTCACTGACTGTGTGCTTA+CGG 0.451326 6.2:-52399869 None:intergenic
GCTTCCAATTCGAACAAGTC+CGG 0.452234 6.2:-52400611 None:intergenic
GTCTCTATTGTCAAAACTGA+TGG 0.462232 6.2:+52400158 MS.gene86849:CDS
GTAGCAATCGGTTTCAGATC+AGG 0.462857 6.2:-52400659 None:intergenic
ATCTGTCGATCTGAACCATA+TGG 0.463638 6.2:+52400304 MS.gene86849:CDS
CTGACTGTGTGCTTACGGCT+AGG 0.464509 6.2:-52399864 None:intergenic
GCCAAAGTGAAGCATGTTGC+TGG 0.465578 6.2:+52400893 MS.gene86849:CDS
TGACTGTGTGCTTACGGCTA+GGG 0.472706 6.2:-52399863 None:intergenic
CCTGAACCCAGATGTCCATA+TGG 0.477535 6.2:-52400319 None:intergenic
CAGGGTATAGGTAGCTATCT+AGG 0.481401 6.2:+52400383 MS.gene86849:CDS
AAATTTAAGTATGAGAAACT+TGG 0.483004 6.2:+52400539 MS.gene86849:CDS
GTTGCGGCCAGCAGCAGGCA+TGG 0.483148 6.2:-52400762 None:intergenic
TTACATCATTTGCTGCTGAT+GGG 0.483354 6.2:+52401116 MS.gene86849:CDS
CCGGGCAAACCTTGTCTGAA+TGG 0.485324 6.2:-52400592 None:intergenic
CCAGCAATATTGAGACTTGA+GGG 0.485345 6.2:-52399972 None:intergenic
TCAGATTCCTCAATTGTAAA+CGG 0.486594 6.2:-52401022 None:intergenic
TGTTACAGGTGCGGACTGCT+TGG 0.488132 6.2:+52400569 MS.gene86849:CDS
TGCCTATTTCAGCGAGCGTA+TGG 0.489265 6.2:+52400109 MS.gene86849:intron
GCTGGCCGTGTTCTTGATGA+TGG 0.492615 6.2:+52400218 MS.gene86849:CDS
CAGAACTTAAGAAGAGAAAG+AGG 0.495062 6.2:+52401068 MS.gene86849:CDS
TGGTTCAGATCGACAGATGT+AGG 0.495803 6.2:-52400299 None:intergenic
CCATTCAGACAAGGTTTGCC+CGG 0.499540 6.2:+52400592 MS.gene86849:CDS
GCGAGCGTATGGCTCGTGCT+TGG 0.503834 6.2:+52400120 MS.gene86849:intron
ATTACATCATTTGCTGCTGA+TGG 0.505133 6.2:+52401115 MS.gene86849:CDS
TGGAAAGTCCGTGTTAGTAC+TGG 0.506120 6.2:+52400500 MS.gene86849:CDS
TGCTGTAATCAACCAAATGT+TGG 0.506410 6.2:+52400359 MS.gene86849:CDS
ACCAGCAACATGCTTCACTT+TGG 0.507514 6.2:-52400894 None:intergenic
AGGTAAAAGATGCACTTGAC+AGG 0.509394 6.2:-52400927 None:intergenic
GTTTGTCTCGGCACAACAGA+TGG 0.513246 6.2:-52400728 None:intergenic
GCTGGCCGCAACTTTCATCC+AGG 0.513777 6.2:+52400773 MS.gene86849:CDS
TGAGGATGACCAGGATGCTA+CGG 0.520820 6.2:+52401042 MS.gene86849:CDS
GTGAGTCAAATCTTGTTCCT+GGG 0.521605 6.2:+52399886 MS.gene86849:CDS
TCAATTGTAAACGGAGCAGC+AGG 0.523367 6.2:-52401013 None:intergenic
GAAAATTATCAAACAGCCAT+GGG 0.523499 6.2:-52400241 None:intergenic
TGGTTTAACCATTGCTGAAG+AGG 0.530085 6.2:+52399992 MS.gene86849:CDS
CTTCCAATTCGAACAAGTCC+GGG 0.531082 6.2:-52400610 None:intergenic
TTTGGAACAAGGAGACAGAT+AGG 0.534007 6.2:-52399936 None:intergenic
TGAAAATTATCAAACAGCCA+TGG 0.534262 6.2:-52400242 None:intergenic
TTTGGTTGATTACAGCAAAA+GGG 0.538059 6.2:-52400353 None:intergenic
ATGTAATTGCACCATTCTGA+AGG 0.538475 6.2:-52401099 None:intergenic
TTCCACCATAAAATGGATGC+TGG 0.541941 6.2:+52400200 MS.gene86849:CDS
CCATAGGAACGTTTGGAACA+AGG 0.544450 6.2:-52399947 None:intergenic
TTTGCTGCTGATGGGGATGG+GGG 0.546802 6.2:+52401124 MS.gene86849:CDS
GGTTTAACCATTGCTGAAGA+GGG 0.548896 6.2:+52399993 MS.gene86849:CDS
ACAGGTATCCCAGTGGTGCT+CGG 0.549283 6.2:+52400986 MS.gene86849:CDS
GAAGCTTGGGGAGTTTGTCT+CGG 0.552735 6.2:-52400740 None:intergenic
GAGGATGACCAGGATGCTAC+GGG 0.553031 6.2:+52401043 MS.gene86849:CDS
TCGGCTTCCACTCTCATCAC+AGG 0.554430 6.2:+52400968 MS.gene86849:CDS
TCTCTATTGTCAAAACTGAT+GGG 0.556196 6.2:+52400159 MS.gene86849:CDS
GGACAACCCCAGGTTGGATA+CGG 0.557706 6.2:-52400277 None:intergenic
GGTTAAACCCTCTTCAGCAA+TGG 0.558439 6.2:-52400000 None:intergenic
ACCAGCAATATTGAGACTTG+AGG 0.558584 6.2:-52399973 None:intergenic
TGAGGAATCTGAGGATGACC+AGG 0.560994 6.2:+52401033 MS.gene86849:CDS
TCATGGACCGTATCCAACCT+GGG 0.563367 6.2:+52400270 MS.gene86849:CDS
GTTCTGAACCCGTAGCATCC+TGG 0.565500 6.2:-52401051 None:intergenic
TTGAGACTTGAGGGATCCAT+AGG 0.568944 6.2:-52399963 None:intergenic
GGTTCAGATCGACAGATGTA+GGG 0.573327 6.2:-52400298 None:intergenic
GAACCAATACGAGTAGCAAT+CGG 0.576710 6.2:-52400671 None:intergenic
TTCGAATTGGAAGCTGATGA+CGG 0.577920 6.2:+52400620 MS.gene86849:CDS
TCAATCGCATATAAGTGTTG+TGG 0.585546 6.2:-52400439 None:intergenic
TCTGAACCATATGGACATCT+GGG 0.585737 6.2:+52400313 MS.gene86849:CDS
ATAGGTTCAAATGTTAGTCA+GGG 0.586644 6.2:+52401157 MS.gene86849:CDS
CAGGTAAACCGAGCACCACT+GGG 0.589111 6.2:-52400994 None:intergenic
ACCGCACTAGCAGCACCACC+TGG 0.589399 6.2:-52400791 None:intergenic
TACATCATTTGCTGCTGATG+GGG 0.595588 6.2:+52401117 MS.gene86849:CDS
TTTGTCTCGGCACAACAGAT+GGG 0.598784 6.2:-52400727 None:intergenic
GTTGATTACAGCAAAAGGGA+AGG 0.604866 6.2:-52400349 None:intergenic
GTGTTCTTGATGATGGCCCA+TGG 0.605740 6.2:+52400225 MS.gene86849:CDS
AATCAACCAAATGTTGGTCA+GGG 0.609202 6.2:+52400365 MS.gene86849:CDS
AGGATGATGACCCTTCAGAA+TGG 0.611730 6.2:+52401088 MS.gene86849:CDS
TCCAGGTGGTGCTGCTAGTG+CGG 0.614412 6.2:+52400790 MS.gene86849:CDS
CGTAATCACCAGTACTAACA+CGG 0.615539 6.2:-52400508 None:intergenic
ACTGCTTGGCCATTCAGACA+AGG 0.615646 6.2:+52400583 MS.gene86849:CDS
CACCACCTGGATGAAAGTTG+CGG 0.616021 6.2:-52400778 None:intergenic
CTCTATTGTCAAAACTGATG+GGG 0.617557 6.2:+52400160 MS.gene86849:CDS
GGCCGCAACTTTCATCCAGG+TGG 0.619748 6.2:+52400776 MS.gene86849:CDS
GAGACAGATAGGAAAACGAG+TGG 0.620293 6.2:-52399925 None:intergenic
CAACATGCTTCACTTTGGCG+AGG 0.622470 6.2:-52400889 None:intergenic
GTTTACAATTGAGGAATCTG+AGG 0.625526 6.2:+52401024 MS.gene86849:CDS
AAAGATGCACTTGACAGGTA+CGG 0.627868 6.2:-52400922 None:intergenic
GCAGCAGGCATGGAAGCTTG+GGG 0.628993 6.2:-52400752 None:intergenic
ACAGATGTAGGGACAACCCC+AGG 0.631187 6.2:-52400287 None:intergenic
GAAAGACCTACAGAAGCAGA+AGG 0.635694 6.2:-52400947 None:intergenic
TCGCATATAAGTGTTGTGGA+TGG 0.641221 6.2:-52400435 None:intergenic
CTCGGCACAACAGATGGGAT+AGG 0.642910 6.2:-52400722 None:intergenic
GTTCAAATGTTAGTCAGGGG+GGG 0.646651 6.2:+52401161 MS.gene86849:CDS
TAGGTTCAAATGTTAGTCAG+GGG 0.648436 6.2:+52401158 MS.gene86849:CDS
GATGACGGAGTTCGTAATTG+GGG 0.648553 6.2:+52400635 MS.gene86849:CDS
TTTCAATGATCGTATGCTAG+CGG 0.655034 6.2:+52400817 MS.gene86849:CDS
ATGTAGGGACAACCCCAGGT+TGG 0.658503 6.2:-52400283 None:intergenic
TGTAATTGCACCATTCTGAA+GGG 0.659743 6.2:-52401098 None:intergenic
AGGTTCAAATGTTAGTCAGG+GGG 0.661132 6.2:+52401159 MS.gene86849:CDS
ATGGGCCATCATCAAGAACA+CGG 0.664079 6.2:-52400223 None:intergenic
TGGGATACCTGTGATGAGAG+TGG 0.673340 6.2:-52400975 None:intergenic
GGTTCAAATGTTAGTCAGGG+GGG 0.675665 6.2:+52401160 MS.gene86849:CDS
TAGTTTCAGACTCAGTGGCG+AGG 0.677757 6.2:-52400028 None:intergenic
GAAACAGAAACGAAACACCC+AGG 0.680562 6.2:-52399903 None:intergenic
CATGGACCGTATCCAACCTG+GGG 0.681438 6.2:+52400271 MS.gene86849:CDS
TCTCATCACAGGTATCCCAG+TGG 0.766548 6.2:+52400979 MS.gene86849:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTAAGTATGAGAAACT+TGG + chr6.2:52400539-52400558 MS.gene86849:CDS 20.0%
!!! TGATAATTTTCATATTGTCA+TGG + chr6.2:52400253-52400272 MS.gene86849:CDS 20.0%
!!! AACTTGGAATTTTTTGTTAC+AGG + chr6.2:52400555-52400574 MS.gene86849:CDS 25.0%
AATAGGTTCAAATGTTAGTC+AGG + chr6.2:52401156-52401175 MS.gene86849:CDS 30.0%
ATAGGTTCAAATGTTAGTCA+GGG + chr6.2:52401157-52401176 MS.gene86849:CDS 30.0%
ATTTGGTTGATTACAGCAAA+AGG - chr6.2:52400357-52400376 None:intergenic 30.0%
GAAAATTATCAAACAGCCAT+GGG - chr6.2:52400244-52400263 None:intergenic 30.0%
TCAGATTCCTCAATTGTAAA+CGG - chr6.2:52401025-52401044 None:intergenic 30.0%
TCTCTATTGTCAAAACTGAT+GGG + chr6.2:52400159-52400178 MS.gene86849:CDS 30.0%
TGAAAATTATCAAACAGCCA+TGG - chr6.2:52400245-52400264 None:intergenic 30.0%
TGCTAATAACAAATGGCTTA+AGG + chr6.2:52400697-52400716 MS.gene86849:CDS 30.0%
TTTGGTTGATTACAGCAAAA+GGG - chr6.2:52400356-52400375 None:intergenic 30.0%
! TTTTCAGTTCCACCATAAAA+TGG + chr6.2:52400193-52400212 MS.gene86849:CDS 30.0%
!! TTTTATGGTGGAACTGAAAA+AGG - chr6.2:52400193-52400212 None:intergenic 30.0%
AATCAACCAAATGTTGGTCA+GGG + chr6.2:52400365-52400384 MS.gene86849:CDS 35.0%
ATGTAATTGCACCATTCTGA+AGG - chr6.2:52401102-52401121 None:intergenic 35.0%
CAGAACTTAAGAAGAGAAAG+AGG + chr6.2:52401068-52401087 MS.gene86849:CDS 35.0%
CTCTATTGTCAAAACTGATG+GGG + chr6.2:52400160-52400179 MS.gene86849:CDS 35.0%
GTCTCTATTGTCAAAACTGA+TGG + chr6.2:52400158-52400177 MS.gene86849:CDS 35.0%
GTTCAGCTGCTAATAACAAA+TGG + chr6.2:52400690-52400709 MS.gene86849:CDS 35.0%
GTTTACAATTGAGGAATCTG+AGG + chr6.2:52401024-52401043 MS.gene86849:CDS 35.0%
TAATCAACCAAATGTTGGTC+AGG + chr6.2:52400364-52400383 MS.gene86849:CDS 35.0%
TAGGTTCAAATGTTAGTCAG+GGG + chr6.2:52401158-52401177 MS.gene86849:CDS 35.0%
TGCTGTAATCAACCAAATGT+TGG + chr6.2:52400359-52400378 MS.gene86849:CDS 35.0%
TGTAATTGCACCATTCTGAA+GGG - chr6.2:52401101-52401120 None:intergenic 35.0%
TTTCAATGATCGTATGCTAG+CGG + chr6.2:52400817-52400836 MS.gene86849:CDS 35.0%
! AAGTGTTGTGGATGGTATTA+CGG - chr6.2:52400430-52400449 None:intergenic 35.0%
! ATTACATCATTTGCTGCTGA+TGG + chr6.2:52401115-52401134 MS.gene86849:CDS 35.0%
! TACGGTCATCATAGGTTTTA+AGG - chr6.2:52400412-52400431 None:intergenic 35.0%
! TCAATCGCATATAAGTGTTG+TGG - chr6.2:52400442-52400461 None:intergenic 35.0%
! TTACATCATTTGCTGCTGAT+GGG + chr6.2:52401116-52401135 MS.gene86849:CDS 35.0%
!!! ACTTTCCACTCTTTTTTCAG+TGG - chr6.2:52400488-52400507 None:intergenic 35.0%
!!! CTTTCCACTCTTTTTTCAGT+GGG - chr6.2:52400487-52400506 None:intergenic 35.0%
!!! GGAATTTTTTGTTACAGGTG+CGG + chr6.2:52400560-52400579 MS.gene86849:CDS 35.0%
AAACCGATTGCTACTCGTAT+TGG + chr6.2:52400668-52400687 MS.gene86849:CDS 40.0%
AAAGATGCACTTGACAGGTA+CGG - chr6.2:52400925-52400944 None:intergenic 40.0%
ACCAGCAATATTGAGACTTG+AGG - chr6.2:52399976-52399995 None:intergenic 40.0%
AGGTAAAAGATGCACTTGAC+AGG - chr6.2:52400930-52400949 None:intergenic 40.0%
AGGTTCAAATGTTAGTCAGG+GGG + chr6.2:52401159-52401178 MS.gene86849:CDS 40.0%
AGTGAGTCAAATCTTGTTCC+TGG + chr6.2:52399885-52399904 MS.gene86849:CDS 40.0%
ATCTGAACCATATGGACATC+TGG + chr6.2:52400312-52400331 MS.gene86849:CDS 40.0%
ATCTGTCGATCTGAACCATA+TGG + chr6.2:52400304-52400323 MS.gene86849:CDS 40.0%
CCAGCAATATTGAGACTTGA+GGG - chr6.2:52399975-52399994 None:intergenic 40.0%
CGTAATCACCAGTACTAACA+CGG - chr6.2:52400511-52400530 None:intergenic 40.0%
GAACCAATACGAGTAGCAAT+CGG - chr6.2:52400674-52400693 None:intergenic 40.0%
GATGGTATTACGGTCATCAT+AGG - chr6.2:52400420-52400439 None:intergenic 40.0%
GGTGGAACTGAAAAAGGAAA+CGG - chr6.2:52400187-52400206 None:intergenic 40.0%
GGTTTAACCATTGCTGAAGA+GGG + chr6.2:52399993-52400012 MS.gene86849:CDS 40.0%
GTGAGTCAAATCTTGTTCCT+GGG + chr6.2:52399886-52399905 MS.gene86849:CDS 40.0%
GTTGATTACAGCAAAAGGGA+AGG - chr6.2:52400352-52400371 None:intergenic 40.0%
TCTGAACCATATGGACATCT+GGG + chr6.2:52400313-52400332 MS.gene86849:CDS 40.0%
TGGTTTAACCATTGCTGAAG+AGG + chr6.2:52399992-52400011 MS.gene86849:CDS 40.0%
TTCCACCATAAAATGGATGC+TGG + chr6.2:52400200-52400219 MS.gene86849:CDS 40.0%
TTCGAATTGGAAGCTGATGA+CGG + chr6.2:52400620-52400639 MS.gene86849:CDS 40.0%
TTCTGCTTCTGTAGGTCTTT+CGG + chr6.2:52400949-52400968 MS.gene86849:CDS 40.0%
! CTGTTTGGTATGACGTGTTT+TGG + chr6.2:52400067-52400086 MS.gene86849:intron 40.0%
! CTTTTACCTTCTGCTTCTGT+AGG + chr6.2:52400941-52400960 MS.gene86849:CDS 40.0%
! GATGGGGGAACTTTTGTAAT+AGG + chr6.2:52401139-52401158 MS.gene86849:CDS 40.0%
! TACATCATTTGCTGCTGATG+GGG + chr6.2:52401117-52401136 MS.gene86849:CDS 40.0%
! TCGCATATAAGTGTTGTGGA+TGG - chr6.2:52400438-52400457 None:intergenic 40.0%
! TTTGGAACAAGGAGACAGAT+AGG - chr6.2:52399939-52399958 None:intergenic 40.0%
!! ACGGCTAGGGTTTTGTATTT+GGG - chr6.2:52399853-52399872 None:intergenic 40.0%
!! TACGGCTAGGGTTTTGTATT+TGG - chr6.2:52399854-52399873 None:intergenic 40.0%
!! TGATGACGGAGTTCGTAATT+GGG + chr6.2:52400634-52400653 MS.gene86849:CDS 40.0%
ACCAGCAACATGCTTCACTT+TGG - chr6.2:52400897-52400916 None:intergenic 45.0%
AGGATGATGACCCTTCAGAA+TGG + chr6.2:52401088-52401107 MS.gene86849:CDS 45.0%
ATGGGCCATCATCAAGAACA+CGG - chr6.2:52400226-52400245 None:intergenic 45.0%
CAGGGTATAGGTAGCTATCT+AGG + chr6.2:52400383-52400402 MS.gene86849:CDS 45.0%
CCAAATGTTGGTCAGGGTAT+AGG + chr6.2:52400371-52400390 MS.gene86849:CDS 45.0%
CCCTCAAGTCTCAATATTGC+TGG + chr6.2:52399972-52399991 MS.gene86849:CDS 45.0%
CCTATACCCTGACCAACATT+TGG - chr6.2:52400374-52400393 None:intergenic 45.0%
CCTTGTTCCAAACGTTCCTA+TGG + chr6.2:52399947-52399966 MS.gene86849:CDS 45.0%
CTGTCCCACTGAAAAAAGAG+TGG + chr6.2:52400480-52400499 MS.gene86849:CDS 45.0%
CTTCCAATTCGAACAAGTCC+GGG - chr6.2:52400613-52400632 None:intergenic 45.0%
GAAACAGAAACGAAACACCC+AGG - chr6.2:52399906-52399925 None:intergenic 45.0%
GAAAGACCTACAGAAGCAGA+AGG - chr6.2:52400950-52400969 None:intergenic 45.0%
GAGACAGATAGGAAAACGAG+TGG - chr6.2:52399928-52399947 None:intergenic 45.0%
GCTTCCAATTCGAACAAGTC+CGG - chr6.2:52400614-52400633 None:intergenic 45.0%
GGTTAAACCCTCTTCAGCAA+TGG - chr6.2:52400003-52400022 None:intergenic 45.0%
GGTTCAAATGTTAGTCAGGG+GGG + chr6.2:52401160-52401179 MS.gene86849:CDS 45.0%
GGTTCAGATCGACAGATGTA+GGG - chr6.2:52400301-52400320 None:intergenic 45.0%
GTCATACCAAACAGTGATCG+AGG - chr6.2:52400061-52400080 None:intergenic 45.0%
GTTCAAATGTTAGTCAGGGG+GGG + chr6.2:52401161-52401180 MS.gene86849:CDS 45.0%
TACCAAACAGTGATCGAGGA+TGG - chr6.2:52400057-52400076 None:intergenic 45.0%
TCAATTGTAAACGGAGCAGC+AGG - chr6.2:52401016-52401035 None:intergenic 45.0%
TGCTGCTCCGTTTACAATTG+AGG + chr6.2:52401015-52401034 MS.gene86849:CDS 45.0%
TGGAAAGTCCGTGTTAGTAC+TGG + chr6.2:52400500-52400519 MS.gene86849:CDS 45.0%
TGGTTCAGATCGACAGATGT+AGG - chr6.2:52400302-52400321 None:intergenic 45.0%
TTGAGACTTGAGGGATCCAT+AGG - chr6.2:52399966-52399985 None:intergenic 45.0%
TTTGGCTGATCGTGAGATTC+AGG + chr6.2:52400085-52400104 MS.gene86849:intron 45.0%
TTTGTCTCGGCACAACAGAT+GGG - chr6.2:52400730-52400749 None:intergenic 45.0%
! ACTCACTGACTGTGTGCTTA+CGG - chr6.2:52399872-52399891 None:intergenic 45.0%
! CCATAGGAACGTTTGGAACA+AGG - chr6.2:52399950-52399969 None:intergenic 45.0%
! GGTGTTAGTTTCAGACTCAG+TGG - chr6.2:52400036-52400055 None:intergenic 45.0%
! GTAGCAATCGGTTTCAGATC+AGG - chr6.2:52400662-52400681 None:intergenic 45.0%
!! CTGATGACGGAGTTCGTAAT+TGG + chr6.2:52400633-52400652 MS.gene86849:CDS 45.0%
!! GATGACGGAGTTCGTAATTG+GGG + chr6.2:52400635-52400654 MS.gene86849:CDS 45.0%
ACAATAGAGACTCGCTTGCC+TGG - chr6.2:52400149-52400168 None:intergenic 50.0%
ACTGCTTGGCCATTCAGACA+AGG + chr6.2:52400583-52400602 MS.gene86849:CDS 50.0%
AGACTCAGTGGCGAGGTTTA+GGG - chr6.2:52400024-52400043 None:intergenic 50.0%
AGCCATACGCTCGCTGAAAT+AGG - chr6.2:52400114-52400133 None:intergenic 50.0%
CAACATGCTTCACTTTGGCG+AGG - chr6.2:52400892-52400911 None:intergenic 50.0%
CACCACCTGGATGAAAGTTG+CGG - chr6.2:52400781-52400800 None:intergenic 50.0%
CACCATCCTCGATCACTGTT+TGG + chr6.2:52400052-52400071 MS.gene86849:intron 50.0%
CCATATGGACATCTGGGTTC+AGG + chr6.2:52400319-52400338 MS.gene86849:CDS 50.0%
CCATTCAGACAAGGTTTGCC+CGG + chr6.2:52400592-52400611 MS.gene86849:CDS 50.0%
CCTGAACCCAGATGTCCATA+TGG - chr6.2:52400322-52400341 None:intergenic 50.0%
GAAGCTTGGGGAGTTTGTCT+CGG - chr6.2:52400743-52400762 None:intergenic 50.0%
GAGGGATCCATAGGAACGTT+TGG - chr6.2:52399957-52399976 None:intergenic 50.0%
GCCAAAGTGAAGCATGTTGC+TGG + chr6.2:52400893-52400912 MS.gene86849:CDS 50.0%
GTTTGTCTCGGCACAACAGA+TGG - chr6.2:52400731-52400750 None:intergenic 50.0%
TAGTTTCAGACTCAGTGGCG+AGG - chr6.2:52400031-52400050 None:intergenic 50.0%
TCATGGACCGTATCCAACCT+GGG + chr6.2:52400270-52400289 MS.gene86849:CDS 50.0%
TCTCATCACAGGTATCCCAG+TGG + chr6.2:52400979-52400998 MS.gene86849:CDS 50.0%
TGACTGTGTGCTTACGGCTA+GGG - chr6.2:52399866-52399885 None:intergenic 50.0%
TGAGGAATCTGAGGATGACC+AGG + chr6.2:52401033-52401052 MS.gene86849:CDS 50.0%
TGAGGATGACCAGGATGCTA+CGG + chr6.2:52401042-52401061 MS.gene86849:CDS 50.0%
TGCCTATTTCAGCGAGCGTA+TGG + chr6.2:52400109-52400128 MS.gene86849:intron 50.0%
TGGGATACCTGTGATGAGAG+TGG - chr6.2:52400978-52400997 None:intergenic 50.0%
! ATTTGCTGCTGATGGGGATG+GGG + chr6.2:52401123-52401142 MS.gene86849:CDS 50.0%
! CACGGCCAGCATCCATTTTA+TGG - chr6.2:52400208-52400227 None:intergenic 50.0%
! CATTTGCTGCTGATGGGGAT+GGG + chr6.2:52401122-52401141 MS.gene86849:CDS 50.0%
! GACATCTGGGTTCAGGTTCA+TGG + chr6.2:52400326-52400345 MS.gene86849:CDS 50.0%
! GGCCAGCATCCATTTTATGG+TGG - chr6.2:52400205-52400224 None:intergenic 50.0%
! TCATTTGCTGCTGATGGGGA+TGG + chr6.2:52401121-52401140 MS.gene86849:CDS 50.0%
! TTGCCCGGACTTGTTCGAAT+TGG + chr6.2:52400607-52400626 MS.gene86849:CDS 50.0%
!! GTGTTCTTGATGATGGCCCA+TGG + chr6.2:52400225-52400244 MS.gene86849:CDS 50.0%
!!! CACTTTGGCGAGGTTTTGAG+CGG - chr6.2:52400882-52400901 None:intergenic 50.0%
ACAGATGTAGGGACAACCCC+AGG - chr6.2:52400290-52400309 None:intergenic 55.0%
ACAGGTATCCCAGTGGTGCT+CGG + chr6.2:52400986-52401005 MS.gene86849:CDS 55.0%
AGCAGCAGGCATGGAAGCTT+GGG - chr6.2:52400756-52400775 None:intergenic 55.0%
ATGTAGGGACAACCCCAGGT+TGG - chr6.2:52400286-52400305 None:intergenic 55.0%
CAGACTCAGTGGCGAGGTTT+AGG - chr6.2:52400025-52400044 None:intergenic 55.0%
CAGGTAAACCGAGCACCACT+GGG - chr6.2:52400997-52401016 None:intergenic 55.0%
CATGGACCGTATCCAACCTG+GGG + chr6.2:52400271-52400290 MS.gene86849:CDS 55.0%
CCGGGCAAACCTTGTCTGAA+TGG - chr6.2:52400595-52400614 None:intergenic 55.0%
CTCGGCACAACAGATGGGAT+AGG - chr6.2:52400725-52400744 None:intergenic 55.0%
CTGACTGTGTGCTTACGGCT+AGG - chr6.2:52399867-52399886 None:intergenic 55.0%
GAGGATGACCAGGATGCTAC+GGG + chr6.2:52401043-52401062 MS.gene86849:CDS 55.0%
GCTGGCCGTGTTCTTGATGA+TGG + chr6.2:52400218-52400237 MS.gene86849:CDS 55.0%
GTCATGGACCGTATCCAACC+TGG + chr6.2:52400269-52400288 MS.gene86849:CDS 55.0%
TCGGCTTCCACTCTCATCAC+AGG + chr6.2:52400968-52400987 MS.gene86849:CDS 55.0%
TTTGCTGCTGATGGGGATGG+GGG + chr6.2:52401124-52401143 MS.gene86849:CDS 55.0%
! GGACAACCCCAGGTTGGATA+CGG - chr6.2:52400280-52400299 None:intergenic 55.0%
! GTTCTGAACCCGTAGCATCC+TGG - chr6.2:52401054-52401073 None:intergenic 55.0%
! TGTTACAGGTGCGGACTGCT+TGG + chr6.2:52400569-52400588 MS.gene86849:CDS 55.0%
ATGGCTCGTGCTTGGAAGCC+AGG + chr6.2:52400128-52400147 MS.gene86849:CDS 60.0%
CAAGCTTCCATGCCTGCTGC+TGG + chr6.2:52400755-52400774 MS.gene86849:CDS 60.0%
CAGCAGCAGGCATGGAAGCT+TGG - chr6.2:52400757-52400776 None:intergenic 60.0%
GCAGCAGGCATGGAAGCTTG+GGG - chr6.2:52400755-52400774 None:intergenic 60.0%
GCAGGTAAACCGAGCACCAC+TGG - chr6.2:52400998-52401017 None:intergenic 60.0%
GCTGGCCGCAACTTTCATCC+AGG + chr6.2:52400773-52400792 MS.gene86849:CDS 60.0%
GGCCGCAACTTTCATCCAGG+TGG + chr6.2:52400776-52400795 MS.gene86849:CDS 60.0%
TGAAAGTTGCGGCCAGCAGC+AGG - chr6.2:52400770-52400789 None:intergenic 60.0%
!! TCCAGGTGGTGCTGCTAGTG+CGG + chr6.2:52400790-52400809 MS.gene86849:CDS 60.0%
ACCGCACTAGCAGCACCACC+TGG - chr6.2:52400794-52400813 None:intergenic 65.0%
GCGAGCGTATGGCTCGTGCT+TGG + chr6.2:52400120-52400139 MS.gene86849:intron 65.0%
GTTGCGGCCAGCAGCAGGCA+TGG - chr6.2:52400765-52400784 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 52399843 52401195 52399843 ID=MS.gene86849
chr6.2 mRNA 52399843 52401195 52399843 ID=MS.gene86849.t1;Parent=MS.gene86849
chr6.2 exon 52399843 52400039 52399843 ID=MS.gene86849.t1.exon1;Parent=MS.gene86849.t1
chr6.2 CDS 52399843 52400039 52399843 ID=cds.MS.gene86849.t1;Parent=MS.gene86849.t1
chr6.2 exon 52400121 52401195 52400121 ID=MS.gene86849.t1.exon2;Parent=MS.gene86849.t1
chr6.2 CDS 52400121 52401195 52400121 ID=cds.MS.gene86849.t1;Parent=MS.gene86849.t1
Gene Sequence

>MS.gene86849

ATGCTCTCCCAAATACAAAACCCTAGCCGTAAGCACACAGTCAGTGAGTCAAATCTTGTTCCTGGGTGTTTCGTTTCTGTTTCCACTCGTTTTCCTATCTGTCTCCTTGTTCCAAACGTTCCTATGGATCCCTCAAGTCTCAATATTGCTGGTTTAACCATTGCTGAAGAGGGTTTAACCCTAAACCTCGCCACTGAGTCTGAAACTAACACCATCCTCGATCACTGTTTGGTATGACGTGTTTTGGCTGATCGTGAGATTCAGGCTGCCTATTTCAGCGAGCGTATGGCTCGTGCTTGGAAGCCAGGCAAGCGAGTCTCTATTGTCAAAACTGATGGGGACCGTTTCCTTTTTCAGTTCCACCATAAAATGGATGCTGGCCGTGTTCTTGATGATGGCCCATGGCTGTTTGATAATTTTCATATTGTCATGGACCGTATCCAACCTGGGGTTGTCCCTACATCTGTCGATCTGAACCATATGGACATCTGGGTTCAGGTTCATGGCCTTCCCTTTTGCTGTAATCAACCAAATGTTGGTCAGGGTATAGGTAGCTATCTAGGCGTCCTTAAAACCTATGATGACCGTAATACCATCCACAACACTTATATGCGATTGAAAGTTCGTATTGATGTTACTGTCCCACTGAAAAAAGAGTGGAAAGTCCGTGTTAGTACTGGTGATTACGTTACTGTCAAATTTAAGTATGAGAAACTTGGAATTTTTTGTTACAGGTGCGGACTGCTTGGCCATTCAGACAAGGTTTGCCCGGACTTGTTCGAATTGGAAGCTGATGACGGAGTTCGTAATTGGGGACCTGATCTGAAACCGATTGCTACTCGTATTGGTTCAGCTGCTAATAACAAATGGCTTAAGGATCCTATCCCATCTGTTGTGCCGAGACAAACTCCCCAAGCTTCCATGCCTGCTGCTGGCCGCAACTTTCATCCAGGTGGTGCTGCTAGTGCGGTGACTTTCAATGATCGTATGCTAGCGGTTGATTCTCAGATTGCTGCTCTTAAAAATGATCTTCTTGCCGCTCAAAACCTCGCCAAAGTGAAGCATGTTGCTGGTGACAACCGTACCTGTCAAGTGCATCTTTTACCTTCTGCTTCTGTAGGTCTTTCGGCTTCCACTCTCATCACAGGTATCCCAGTGGTGCTCGGTTTACCTGCTGCTCCGTTTACAATTGAGGAATCTGAGGATGACCAGGATGCTACGGGTTCAGAACTTAAGAAGAGAAAGAGGATGATGACCCTTCAGAATGGTGCAATTACATCATTTGCTGCTGATGGGGATGGGGGAACTTTTGTAATAGGTTCAAATGTTAGTCAGGGGGGGATACTGATATGA
Protein sequence

>MS.gene86849.t1

MLSQIQNPSRKHTVSESNLVPGCFVSVSTRFPICLLVPNVPMDPSSLNIAGLTIAEEGLTLNLATDERMARAWKPGKRVSIVKTDGDRFLFQFHHKMDAGRVLDDGPWLFDNFHIVMDRIQPGVVPTSVDLNHMDIWVQVHGLPFCCNQPNVGQGIGSYLGVLKTYDDRNTIHNTYMRLKVRIDVTVPLKKEWKVRVSTGDYVTVKFKYEKLGIFCYRCGLLGHSDKVCPDLFELEADDGVRNWGPDLKPIATRIGSAANNKWLKDPIPSVVPRQTPQASMPAAGRNFHPGGAASAVTFNDRMLAVDSQIAALKNDLLAAQNLAKVKHVAGDNRTCQVHLLPSASVGLSASTLITGIPVVLGLPAAPFTIEESEDDQDATGSELKKRKRMMTLQNGAITSFAADGDGGTFVIGSNVSQGGILI