AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65623

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGCTGCTGTCACTATTT+TGG 0.191730 8.2:+38001293 MS.gene65623:CDS
ATGCAAGTGTCTACTGAAAC+TGG 0.289241 8.2:+38002392 MS.gene65623:CDS
TGGTGGTATGACTCTGAATT+TGG 0.331529 8.2:+38001263 MS.gene65623:CDS
TCATCAGAATTCGCAGCTAT+TGG 0.343043 8.2:-38002020 None:intergenic
AGATGATGGCGTGAGAGGTT+GGG 0.365869 8.2:+38001887 MS.gene65623:CDS
CAACAAATGGTTGCGGAATC+CGG 0.392388 8.2:+38001956 MS.gene65623:CDS
GAACCTATTGGAAGCGCATC+AGG 0.397110 8.2:+38002217 MS.gene65623:CDS
TAGATGATGGCGTGAGAGGT+TGG 0.397686 8.2:+38001886 MS.gene65623:CDS
CAGCAGGATCAGCAGCCTTC+AGG 0.398777 8.2:-38002139 None:intergenic
CCACCTGATGCGCTTCCAAT+AGG 0.407567 8.2:-38002220 None:intergenic
GCGAATTCTGATGAAGCTTC+AGG 0.409462 8.2:+38002029 MS.gene65623:CDS
GGCGTGCCAACCGCTCCTTC+AGG 0.425500 8.2:-38001368 None:intergenic
AAGAGGAAGCGTGGACAGAC+TGG 0.425512 8.2:+38002338 MS.gene65623:CDS
CTGAAGGCTGCTGATCCTGC+TGG 0.429407 8.2:+38002140 MS.gene65623:CDS
TAGGCCGTGTGCTAACTGAT+AGG 0.432167 8.2:+38001328 MS.gene65623:CDS
GATGATGGCGTGAGAGGTTG+GGG 0.433761 8.2:+38001888 MS.gene65623:CDS
GGTATAGTGGACACAGAAGC+AGG 0.465854 8.2:+38002302 MS.gene65623:CDS
GGTGCAGCAACAGCATCAAT+AGG 0.476053 8.2:-38002368 None:intergenic
AAGACAACTTCTCGCAAACT+TGG 0.480845 8.2:+38001921 MS.gene65623:CDS
AGGTAATGACATGAACCTAT+TGG 0.482571 8.2:+38002205 MS.gene65623:CDS
TAGCAATGCTGCGAAGAGTT+CGG 0.485011 8.2:+38002052 MS.gene65623:CDS
TGTTGACTCTGCTGGTATAG+TGG 0.485953 8.2:+38002289 MS.gene65623:CDS
GTTGTCGAGTAAGGGCTAAA+TGG 0.495224 8.2:-38003491 None:intergenic
TCGGAGAAGGCAATGTTGAA+TGG 0.501387 8.2:+38002071 MS.gene65623:CDS
AATCCGGTGAAGTCTGAGAC+AGG 0.505548 8.2:+38001972 MS.gene65623:CDS
TACTGATGAGGTCCTAGCAC+TGG 0.509019 8.2:+38002262 MS.gene65623:CDS
GCTATTGGTGATGCAGCCAC+TGG 0.514217 8.2:-38002005 None:intergenic
CAATCTCGAGGGCCTCACTC+TGG 0.524365 8.2:+38001236 MS.gene65623:CDS
GGAAGCGCATCAGGTGGTGG+TGG 0.525431 8.2:+38002226 MS.gene65623:CDS
CTGGAGTATGTTGACTCTGC+TGG 0.525526 8.2:+38002281 MS.gene65623:CDS
GGTAAATCCGCGCTCAAGGA+AGG 0.526201 8.2:+38002185 MS.gene65623:CDS
TGCCCTGTCTCAGACTTCAC+CGG 0.536182 8.2:-38001975 None:intergenic
TCAAAAGCTTTCCAACCAGC+AGG 0.538297 8.2:-38002155 None:intergenic
CCGTGGTAAATCCGCGCTCA+AGG 0.540853 8.2:+38002181 MS.gene65623:CDS
CAGGGTCTGATCTGAAGAAG+AGG 0.541560 8.2:+38002321 MS.gene65623:CDS
AGGCCGTGTGCTAACTGATA+GGG 0.542710 8.2:+38001329 MS.gene65623:CDS
GTACTGCTGCTAACAACAAA+TGG 0.542940 8.2:+38001943 MS.gene65623:CDS
GAGGGCCTCACTCTGGAAGA+TGG 0.543252 8.2:+38001243 MS.gene65623:CDS
TTTGAGAATGATATAGATGA+TGG 0.548596 8.2:+38001873 MS.gene65623:CDS
ATTGGAAGCGCATCAGGTGG+TGG 0.554402 8.2:+38002223 MS.gene65623:CDS
CGAGTAAGGGCTAAATGGTG+TGG 0.555816 8.2:-38003486 None:intergenic
ATGGAAGAATGACTGTTGTT+CGG 0.566927 8.2:+38002090 MS.gene65623:CDS
CTGCTAACAACAAATGGTTG+CGG 0.567597 8.2:+38001949 MS.gene65623:CDS
TGCAAGTGTCTACTGAAACT+GGG 0.573019 8.2:+38002393 MS.gene65623:CDS
GTCATTACCTTCCTTGAGCG+CGG 0.573343 8.2:-38002192 None:intergenic
GATATAGATGATGGCGTGAG+AGG 0.576019 8.2:+38001882 MS.gene65623:CDS
AGGCTGCTGATCCTGCTGGT+TGG 0.579186 8.2:+38002144 MS.gene65623:CDS
GTTCTTAAGAGTCGTAATCT+CGG 0.579938 8.2:-38002114 None:intergenic
TGCTGCGAAGAGTTCGGAGA+AGG 0.582790 8.2:+38002058 MS.gene65623:CDS
GCAAGTGTCTACTGAAACTG+GGG 0.588502 8.2:+38002394 MS.gene65623:CDS
AGTCAACATACTCCAGTGCT+AGG 0.596029 8.2:-38002274 None:intergenic
GAAGCGCATCAGGTGGTGGT+GGG 0.605582 8.2:+38002227 MS.gene65623:CDS
ACTTACTGCTCCTACTGATG+AGG 0.608160 8.2:+38002250 MS.gene65623:CDS
GACCATGGAGTTCAATCTCG+AGG 0.610850 8.2:+38001224 None:intergenic
ACCATGGAGTTCAATCTCGA+GGG 0.613641 8.2:+38001225 None:intergenic
CCTTGAGCGCGGATTTACCA+CGG 0.614263 8.2:-38002181 None:intergenic
GAGTAAGGGCTAAATGGTGT+GGG 0.618820 8.2:-38003485 None:intergenic
ATTCTCAAACAATTGCTCAC+AGG 0.620948 8.2:-38001859 None:intergenic
GACTCTTAAGAACCGCCTGA+AGG 0.623823 8.2:+38002124 MS.gene65623:CDS
ATTGGTGATGCAGCCACTGG+AGG 0.626696 8.2:-38002002 None:intergenic
GCAGCAACAGCATCAATAGG+AGG 0.626740 8.2:-38002365 None:intergenic
CAGGATCAGCAGCCTTCAGG+CGG 0.627993 8.2:-38002136 None:intergenic
GCCCTCGAGATTGAACTCCA+TGG 0.628707 8.2:-38001226 None:intergenic
GTTTGCGAGAAGTTGTCTTG+AGG 0.631086 8.2:-38001917 None:intergenic
AGTGCTAGGACCTCATCAGT+AGG 0.636244 8.2:-38002260 None:intergenic
GACAGGGCAAGCTCCTCCAG+TGG 0.636415 8.2:+38001989 MS.gene65623:CDS
GATCTGAAGAAGAGGAAGCG+TGG 0.641454 8.2:+38002329 MS.gene65623:CDS
GGTGCGATTCGCCTACCTGA+AGG 0.644091 8.2:+38001353 MS.gene65623:CDS
GTTTCAGTAGACACTTGCAT+AGG 0.646063 8.2:-38002389 None:intergenic
TACCACCATCTTCCAGAGTG+AGG 0.647456 8.2:-38001248 None:intergenic
CCTATTGGAAGCGCATCAGG+TGG 0.648591 8.2:+38002220 MS.gene65623:CDS
GTATAGTGGACACAGAAGCA+GGG 0.662035 8.2:+38002303 MS.gene65623:CDS
CCGTGTGCTAACTGATAGGG+AGG 0.672819 8.2:+38001332 MS.gene65623:CDS
TGCCAACCGCTCCTTCAGGT+AGG 0.675928 8.2:-38001364 None:intergenic
GATTCGCCTACCTGAAGGAG+CGG 0.676843 8.2:+38001358 MS.gene65623:CDS
ATCCGGTGAAGTCTGAGACA+GGG 0.678104 8.2:+38001973 MS.gene65623:CDS
GGCCTCACTCTGGAAGATGG+TGG 0.680079 8.2:+38001246 MS.gene65623:CDS
CCTCCCTATCAGTTAGCACA+CGG 0.697187 8.2:-38001332 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTTCATAAATTCACTATG+TGG - chr8.2:38001693-38001712 None:intergenic 25.0%
! AATTTAAATACGAAAACGCT+TGG + chr8.2:38003265-38003284 MS.gene65623:intron 25.0%
! ACATTGTAACAAATTAAGAC+AGG + chr8.2:38003404-38003423 MS.gene65623:intron 25.0%
! ATTTAAATACGAAAACGCTT+GGG + chr8.2:38003266-38003285 MS.gene65623:intron 25.0%
! CAAATTATTGATCCAGATAA+AGG + chr8.2:38002811-38002830 MS.gene65623:intron 25.0%
! CTTTATCTGGATCAATAATT+TGG - chr8.2:38002813-38002832 None:intergenic 25.0%
! TTTGAGAATGATATAGATGA+TGG + chr8.2:38001873-38001892 MS.gene65623:CDS 25.0%
! TTTGTTTGAACATGAGAAAA+GGG + chr8.2:38002963-38002982 MS.gene65623:intron 25.0%
!!! TTTTGTTTGAACATGAGAAA+AGG + chr8.2:38002962-38002981 MS.gene65623:intron 25.0%
ATACTCTTGAACCATGTAAA+CGG - chr8.2:38003078-38003097 None:intergenic 30.0%
CAAAACAACAATCAAAACCA+AGG - chr8.2:38002693-38002712 None:intergenic 30.0%
GTTTGATGAAACTGATGTAA+CGG + chr8.2:38002487-38002506 MS.gene65623:intron 30.0%
TAAACGGATAACCAATCATA+GGG - chr8.2:38003062-38003081 None:intergenic 30.0%
TTGTTTGAACATGAGAAAAG+GGG + chr8.2:38002964-38002983 MS.gene65623:intron 30.0%
! ATTTTGGATCAATGCTTGAT+AGG + chr8.2:38001309-38001328 MS.gene65623:CDS 30.0%
! TTTACATGGTTCAAGAGTAT+GGG + chr8.2:38003078-38003097 MS.gene65623:intron 30.0%
AAATTCTCAACCTGTGCATT+CGG - chr8.2:38003171-38003190 None:intergenic 35.0%
ACATCAGTTTCATCAAACAC+AGG - chr8.2:38002485-38002504 None:intergenic 35.0%
AGGTAATGACATGAACCTAT+TGG + chr8.2:38002205-38002224 MS.gene65623:CDS 35.0%
ATGGAAGAATGACTGTTGTT+CGG + chr8.2:38002090-38002109 MS.gene65623:CDS 35.0%
ATTCTCAAACAATTGCTCAC+AGG - chr8.2:38001862-38001881 None:intergenic 35.0%
CATCCTTCTTGTGATTAAAC+TGG - chr8.2:38001452-38001471 None:intergenic 35.0%
GATGTATGACAACTGTAACT+TGG + chr8.2:38001500-38001519 MS.gene65623:intron 35.0%
GGCAATTCACAGGTTATTAT+GGG + chr8.2:38002839-38002858 MS.gene65623:intron 35.0%
GTAAACGGATAACCAATCAT+AGG - chr8.2:38003063-38003082 None:intergenic 35.0%
GTTCCAGTTTAATCACAAGA+AGG + chr8.2:38001446-38001465 MS.gene65623:intron 35.0%
GTTCTTAAGAGTCGTAATCT+CGG - chr8.2:38002117-38002136 None:intergenic 35.0%
GTTTACATGGTTCAAGAGTA+TGG + chr8.2:38003077-38003096 MS.gene65623:intron 35.0%
TAATAACCTGTGAATTGCCA+AGG - chr8.2:38002838-38002857 None:intergenic 35.0%
TCGCAAAGAAAGAAAACATC+CGG - chr8.2:38002628-38002647 None:intergenic 35.0%
TGGCAATTCACAGGTTATTA+TGG + chr8.2:38002838-38002857 MS.gene65623:intron 35.0%
TTACAGTTGTCATACATCCA+CGG - chr8.2:38001498-38001517 None:intergenic 35.0%
TTATTATGGGTTTCCGGAAA+GGG + chr8.2:38002852-38002871 MS.gene65623:intron 35.0%
! TGATTGGTTATCCGTTTACA+TGG + chr8.2:38003064-38003083 MS.gene65623:intron 35.0%
!!! TGTTTTGCTGTTAGTAGTTC+TGG + chr8.2:38002706-38002725 MS.gene65623:intron 35.0%
AAGACAACTTCTCGCAAACT+TGG + chr8.2:38001921-38001940 MS.gene65623:CDS 40.0%
AATTGCCAAGGACCTTTATC+TGG - chr8.2:38002826-38002845 None:intergenic 40.0%
ATGCAAGTGTCTACTGAAAC+TGG + chr8.2:38002392-38002411 MS.gene65623:CDS 40.0%
CACAGGTTATTATGGGTTTC+CGG + chr8.2:38002846-38002865 MS.gene65623:intron 40.0%
CAGGTTCACAATTTACCCTT+TGG + chr8.2:38001591-38001610 MS.gene65623:intron 40.0%
CATACTCCTTCAATTCACCA+AGG - chr8.2:38001656-38001675 None:intergenic 40.0%
CCTCGTTCGGTATTATAAAC+CGG + chr8.2:38002606-38002625 MS.gene65623:intron 40.0%
CTGCTAACAACAAATGGTTG+CGG + chr8.2:38001949-38001968 MS.gene65623:CDS 40.0%
CTGTTGTGTTCAAGTACGAA+AGG + chr8.2:38001787-38001806 MS.gene65623:intron 40.0%
CTTGTTGATGTCCCTATGAT+TGG + chr8.2:38003048-38003067 MS.gene65623:intron 40.0%
GAAGATCTTCGTTATGTCCT+TGG + chr8.2:38002673-38002692 MS.gene65623:intron 40.0%
GATCTGTGGCATTATCATCA+TGG + chr8.2:38003321-38003340 MS.gene65623:intron 40.0%
GATGAAACTGATGTAACGGT+GGG + chr8.2:38002491-38002510 MS.gene65623:intron 40.0%
GATTCAGCTCAACATCCTTT+GGG - chr8.2:38001554-38001573 None:intergenic 40.0%
GCTGAAAATGCTATGAATGC+CGG + chr8.2:38001471-38001490 MS.gene65623:intron 40.0%
GGAAAATGAGTCTGTTGTGT+TGG + chr8.2:38002536-38002555 MS.gene65623:intron 40.0%
GTACTGCTGCTAACAACAAA+TGG + chr8.2:38001943-38001962 MS.gene65623:CDS 40.0%
GTGTTCAAGTACGAAAGGTT+GGG + chr8.2:38001792-38001811 MS.gene65623:intron 40.0%
GTTATTATGGGTTTCCGGAA+AGG + chr8.2:38002851-38002870 MS.gene65623:intron 40.0%
GTTTCAGTAGACACTTGCAT+AGG - chr8.2:38002392-38002411 None:intergenic 40.0%
TAGGTATAGCTGCTGTTAGT+GGG + chr8.2:38002426-38002445 MS.gene65623:intron 40.0%
TATTATGGGTTTCCGGAAAG+GGG + chr8.2:38002853-38002872 MS.gene65623:intron 40.0%
TCATCAGAATTCGCAGCTAT+TGG - chr8.2:38002023-38002042 None:intergenic 40.0%
TGATGAAACTGATGTAACGG+TGG + chr8.2:38002490-38002509 MS.gene65623:intron 40.0%
TGATTCAGCTCAACATCCTT+TGG - chr8.2:38001555-38001574 None:intergenic 40.0%
TGCAAGTGTCTACTGAAACT+GGG + chr8.2:38002393-38002412 MS.gene65623:CDS 40.0%
TGGTGGTATGACTCTGAATT+TGG + chr8.2:38001263-38001282 MS.gene65623:CDS 40.0%
TGTGTTCAAGTACGAAAGGT+TGG + chr8.2:38001791-38001810 MS.gene65623:intron 40.0%
TGTTCAAGTACGAAAGGTTG+GGG + chr8.2:38001793-38001812 MS.gene65623:intron 40.0%
TTGATCCAGATAAAGGTCCT+TGG + chr8.2:38002818-38002837 MS.gene65623:intron 40.0%
! CAAAACTGAAGTACCTCGTT+CGG + chr8.2:38002593-38002612 MS.gene65623:intron 40.0%
! CACAACAGACTCATTTTCCT+CGG - chr8.2:38002535-38002554 None:intergenic 40.0%
! TGATGCTGCTGTCACTATTT+TGG + chr8.2:38001293-38001312 MS.gene65623:CDS 40.0%
! TGTTATGCTTGTGGATTGCT+GGG + chr8.2:38001822-38001841 MS.gene65623:intron 40.0%
! TTCTTTGTTTCCGAATGCAC+AGG + chr8.2:38003158-38003177 MS.gene65623:intron 40.0%
!! GAGTATTGGTTCGAGATAGT+TGG + chr8.2:38003133-38003152 MS.gene65623:intron 40.0%
!! GTTAGTAGTTCTGGTAGAAG+TGG + chr8.2:38002715-38002734 MS.gene65623:intron 40.0%
AAAGGTCCTTGGCAATTCAC+AGG + chr8.2:38002829-38002848 MS.gene65623:intron 45.0%
ACTTACTGCTCCTACTGATG+AGG + chr8.2:38002250-38002269 MS.gene65623:CDS 45.0%
AGAATCGTCACTCCTTTGAC+AGG - chr8.2:38001402-38001421 None:intergenic 45.0%
AGAGAAGGATGGCTGATCAA+TGG + chr8.2:38002997-38003016 MS.gene65623:intron 45.0%
AGGTATAGCTGCTGTTAGTG+GGG + chr8.2:38002427-38002446 MS.gene65623:intron 45.0%
AGTCAACATACTCCAGTGCT+AGG - chr8.2:38002277-38002296 None:intergenic 45.0%
ATGACATGGTAGCAGATCTG+TGG + chr8.2:38003307-38003326 MS.gene65623:intron 45.0%
ATGGTCATTCAGTTATCCCC+CGG + chr8.2:38003340-38003359 MS.gene65623:intron 45.0%
CAACAAATGGTTGCGGAATC+CGG + chr8.2:38001956-38001975 MS.gene65623:CDS 45.0%
CATAGGGACATCAACAAGCT+CGG - chr8.2:38003046-38003065 None:intergenic 45.0%
CCGGTTTATAATACCGAACG+AGG - chr8.2:38002609-38002628 None:intergenic 45.0%
CTCCTTCAATTCACCAAGGT+AGG - chr8.2:38001652-38001671 None:intergenic 45.0%
CTTAGTGTGTGTCGTGATCT+AGG + chr8.2:38003450-38003469 MS.gene65623:intron 45.0%
CTTGAGCCACTTCTAACTTG+CGG - chr8.2:38003240-38003259 None:intergenic 45.0%
GAATCGTCACTCCTTTGACA+GGG - chr8.2:38001401-38001420 None:intergenic 45.0%
GAGAAGGATGGCTGATCAAT+GGG + chr8.2:38002998-38003017 MS.gene65623:intron 45.0%
GCAAGTGTCTACTGAAACTG+GGG + chr8.2:38002394-38002413 MS.gene65623:CDS 45.0%
GCGAATTCTGATGAAGCTTC+AGG + chr8.2:38002029-38002048 MS.gene65623:CDS 45.0%
GCTACCATGTCATGAAAACC+AGG - chr8.2:38003300-38003319 None:intergenic 45.0%
GGGACTTTCTGTTATGCTTG+TGG + chr8.2:38001813-38001832 MS.gene65623:intron 45.0%
GGTTGAGAATTTAGTAGCGC+CGG + chr8.2:38003179-38003198 MS.gene65623:intron 45.0%
GTAGGTATAGCTGCTGTTAG+TGG + chr8.2:38002425-38002444 MS.gene65623:intron 45.0%
GTATCACTACCATGGTGCAT+TGG + chr8.2:38002922-38002941 MS.gene65623:intron 45.0%
GTTACAATGTGTCTGACTCC+AGG - chr8.2:38003394-38003413 None:intergenic 45.0%
GTTTGCGAGAAGTTGTCTTG+AGG - chr8.2:38001920-38001939 None:intergenic 45.0%
TAGCAATGCTGCGAAGAGTT+CGG + chr8.2:38002052-38002071 MS.gene65623:CDS 45.0%
TCAAAAGCTTTCCAACCAGC+AGG - chr8.2:38002158-38002177 None:intergenic 45.0%
TCGGAGAAGGCAATGTTGAA+TGG + chr8.2:38002071-38002090 MS.gene65623:CDS 45.0%
TGCCTACCTTGGTGAATTGA+AGG + chr8.2:38001647-38001666 MS.gene65623:intron 45.0%
TGGGTTTCGTCAAGCAGTTT+CGG + chr8.2:38003017-38003036 MS.gene65623:intron 45.0%
TTGGTACCGCAAGTTAGAAG+TGG + chr8.2:38003231-38003250 MS.gene65623:intron 45.0%
! AACAGACTCATTTTCCTCGG+TGG - chr8.2:38002532-38002551 None:intergenic 45.0%
! ACAGACTCATTTTCCTCGGT+GGG - chr8.2:38002531-38002550 None:intergenic 45.0%
! ACCATGGTAGTGATACATCC+CGG - chr8.2:38002918-38002937 None:intergenic 45.0%
! ACCTTCCACTCTTGTTTCAG+CGG - chr8.2:38001741-38001760 None:intergenic 45.0%
! ACTTCTAACTTGCGGTACCA+AGG - chr8.2:38003232-38003251 None:intergenic 45.0%
! ATTTTCTCCGAGGTTTAGCC+CGG + chr8.2:38002896-38002915 MS.gene65623:intron 45.0%
! CTGTTATGCTTGTGGATTGC+TGG + chr8.2:38001821-38001840 MS.gene65623:intron 45.0%
! GAAGCATGGAATTTTCTCCG+AGG + chr8.2:38002886-38002905 MS.gene65623:intron 45.0%
! GATATAGATGATGGCGTGAG+AGG + chr8.2:38001882-38001901 MS.gene65623:CDS 45.0%
! GTTATGCTTGTGGATTGCTG+GGG + chr8.2:38001823-38001842 MS.gene65623:intron 45.0%
!! AGTAGTTCTGGTAGAAGTGG+TGG + chr8.2:38002718-38002737 MS.gene65623:intron 45.0%
!! CGAACGAGGTACTTCAGTTT+TGG - chr8.2:38002595-38002614 None:intergenic 45.0%
!! GGAACCTGGTTTTCATGACA+TGG + chr8.2:38003293-38003312 MS.gene65623:intron 45.0%
!! GTATAGTGGACACAGAAGCA+GGG + chr8.2:38002303-38002322 MS.gene65623:CDS 45.0%
!! TGACAACTGTAACTTGGTGC+TGG + chr8.2:38001506-38001525 MS.gene65623:intron 45.0%
!! TGCTGGTTGGAAAGCTTTTG+AGG + chr8.2:38002157-38002176 MS.gene65623:CDS 45.0%
!! TGTTGACTCTGCTGGTATAG+TGG + chr8.2:38002289-38002308 MS.gene65623:CDS 45.0%
!! TTCTGGTAGAAGTGGTGGAT+TGG + chr8.2:38002723-38002742 MS.gene65623:intron 45.0%
!!! TCAGTTTTGGAACTACACGC+AGG - chr8.2:38002582-38002601 None:intergenic 45.0%
AAGTCACCACCAATGCACCA+TGG - chr8.2:38002934-38002953 None:intergenic 50.0%
AGCTGAATCACCTGGACATG+TGG + chr8.2:38001565-38001584 MS.gene65623:intron 50.0%
AGGCCGTGTGCTAACTGATA+GGG + chr8.2:38001329-38001348 MS.gene65623:CDS 50.0%
AGTGCTAGGACCTCATCAGT+AGG - chr8.2:38002263-38002282 None:intergenic 50.0%
AGTGTCTACTGAAACTGGGG+TGG + chr8.2:38002397-38002416 MS.gene65623:intron 50.0%
ATACATCCCGGGCTAAACCT+CGG - chr8.2:38002906-38002925 None:intergenic 50.0%
ATACGAAAACGCTTGGGCGT+TGG + chr8.2:38003272-38003291 MS.gene65623:intron 50.0%
ATGGGATGGTGATCAGAAGC+CGG - chr8.2:38003201-38003220 None:intergenic 50.0%
CAAAGCCGCTGAAACAAGAG+TGG + chr8.2:38001733-38001752 MS.gene65623:intron 50.0%
CAAAGGAGTGACGATTCTGC+AGG + chr8.2:38001404-38001423 MS.gene65623:intron 50.0%
CAGGGTCTGATCTGAAGAAG+AGG + chr8.2:38002321-38002340 MS.gene65623:CDS 50.0%
CCTCCCTATCAGTTAGCACA+CGG - chr8.2:38001335-38001354 None:intergenic 50.0%
CGCAAGTTAGAAGTGGCTCA+AGG + chr8.2:38003238-38003257 MS.gene65623:intron 50.0%
GAACCTGAATCCACATGTCC+AGG - chr8.2:38001578-38001597 None:intergenic 50.0%
GACTCTTAAGAACCGCCTGA+AGG + chr8.2:38002124-38002143 MS.gene65623:CDS 50.0%
GATCTGAAGAAGAGGAAGCG+TGG + chr8.2:38002329-38002348 MS.gene65623:CDS 50.0%
GCAGCAACAGCATCAATAGG+AGG - chr8.2:38002368-38002387 None:intergenic 50.0%
GGAACGGTTGGACAGAGTAT+TGG + chr8.2:38003119-38003138 MS.gene65623:intron 50.0%
GGAACGTATCAACCCAGGTA+TGG + chr8.2:38001527-38001546 MS.gene65623:intron 50.0%
GGATGTTGAGCTGAATCACC+TGG + chr8.2:38001557-38001576 MS.gene65623:intron 50.0%
GGTACCAAGGAGAGCAGAAT+GGG - chr8.2:38003219-38003238 None:intergenic 50.0%
GGTATAGCTGCTGTTAGTGG+GGG + chr8.2:38002428-38002447 MS.gene65623:intron 50.0%
GGTGCAGCAACAGCATCAAT+AGG - chr8.2:38002371-38002390 None:intergenic 50.0%
GTATAGCTGCTGTTAGTGGG+GGG + chr8.2:38002429-38002448 MS.gene65623:intron 50.0%
GTCATTACCTTCCTTGAGCG+CGG - chr8.2:38002195-38002214 None:intergenic 50.0%
GTGCACGTTCAAGAGAAGGA+TGG + chr8.2:38002986-38003005 MS.gene65623:intron 50.0%
GTGTCTACTGAAACTGGGGT+GGG + chr8.2:38002398-38002417 MS.gene65623:intron 50.0%
GTTGTCATACATCCACGGAC+CGG - chr8.2:38001493-38001512 None:intergenic 50.0%
TACCACCATCTTCCAGAGTG+AGG - chr8.2:38001251-38001270 None:intergenic 50.0%
TACTGATGAGGTCCTAGCAC+TGG + chr8.2:38002262-38002281 MS.gene65623:CDS 50.0%
TAGGCCGTGTGCTAACTGAT+AGG + chr8.2:38001328-38001347 MS.gene65623:CDS 50.0%
TATAGCTGCTGTTAGTGGGG+GGG + chr8.2:38002430-38002449 MS.gene65623:intron 50.0%
TCACCTGGACATGTGGATTC+AGG + chr8.2:38001572-38001591 MS.gene65623:intron 50.0%
TCACTACCATGGTGCATTGG+TGG + chr8.2:38002925-38002944 MS.gene65623:intron 50.0%
TGGAACAGAAATCGCCCTTC+CGG - chr8.2:38001432-38001451 None:intergenic 50.0%
! AATCCGGTGAAGTCTGAGAC+AGG + chr8.2:38001972-38001991 MS.gene65623:CDS 50.0%
! AGATGATGGCGTGAGAGGTT+GGG + chr8.2:38001887-38001906 MS.gene65623:CDS 50.0%
! ATCCGGTGAAGTCTGAGACA+GGG + chr8.2:38001973-38001992 MS.gene65623:CDS 50.0%
! CCATGGTAGTGATACATCCC+GGG - chr8.2:38002917-38002936 None:intergenic 50.0%
! GAACCTATTGGAAGCGCATC+AGG + chr8.2:38002217-38002236 MS.gene65623:CDS 50.0%
! GATGCTATTGGTGCCTACCT+TGG + chr8.2:38001636-38001655 MS.gene65623:intron 50.0%
! TACCCTTTGGCTTCATGCAG+CGG + chr8.2:38001604-38001623 MS.gene65623:intron 50.0%
! TAGATGATGGCGTGAGAGGT+TGG + chr8.2:38001886-38001905 MS.gene65623:CDS 50.0%
! TATGGGTACTGTCCGTGAAG+TGG + chr8.2:38003095-38003114 MS.gene65623:intron 50.0%
! TTTTCTCCGAGGTTTAGCCC+GGG + chr8.2:38002897-38002916 MS.gene65623:intron 50.0%
!! ACATCCTTTGGGACCATACC+TGG - chr8.2:38001543-38001562 None:intergenic 50.0%
!! CATCCTTTGGGACCATACCT+GGG - chr8.2:38001542-38001561 None:intergenic 50.0%
!! CTGGAGTATGTTGACTCTGC+TGG + chr8.2:38002281-38002300 MS.gene65623:CDS 50.0%
!! GGTATAGTGGACACAGAAGC+AGG + chr8.2:38002302-38002321 MS.gene65623:CDS 50.0%
!! TGGAAAGCTTTTGAGGACCG+TGG + chr8.2:38002164-38002183 MS.gene65623:CDS 50.0%
ATCAGCGCACAATTCCAGCC+GGG - chr8.2:38003361-38003380 None:intergenic 55.0%
ATGCTATGAATGCCGGTCCG+TGG + chr8.2:38001478-38001497 MS.gene65623:intron 55.0%
CAACCCAGGTATGGTCCCAA+AGG + chr8.2:38001536-38001555 MS.gene65623:intron 55.0%
CATCAGCGCACAATTCCAGC+CGG - chr8.2:38003362-38003381 None:intergenic 55.0%
CCAACCGTTCCTCCACTTCA+CGG - chr8.2:38003110-38003129 None:intergenic 55.0%
CCAAGGAGAGCAGAATGGGA+TGG - chr8.2:38003215-38003234 None:intergenic 55.0%
CCACCTGATGCGCTTCCAAT+AGG - chr8.2:38002223-38002242 None:intergenic 55.0%
CCATCCCATTCTGCTCTCCT+TGG + chr8.2:38003212-38003231 MS.gene65623:intron 55.0%
CCCGGGATGTATCACTACCA+TGG + chr8.2:38002914-38002933 MS.gene65623:intron 55.0%
CCGTGTGCTAACTGATAGGG+AGG + chr8.2:38001332-38001351 MS.gene65623:CDS 55.0%
CCTTGAGCGCGGATTTACCA+CGG - chr8.2:38002184-38002203 None:intergenic 55.0%
CGGCGTGTTGGAGATGCTAT+TGG + chr8.2:38001624-38001643 MS.gene65623:intron 55.0%
CGGTACCAAGGAGAGCAGAA+TGG - chr8.2:38003220-38003239 None:intergenic 55.0%
CTGTCCGTGAAGTGGAGGAA+CGG + chr8.2:38003103-38003122 MS.gene65623:intron 55.0%
CTGTTGTGTTGGAACTGCCG+AGG + chr8.2:38002547-38002566 MS.gene65623:intron 55.0%
GATTCGCCTACCTGAAGGAG+CGG + chr8.2:38001358-38001377 MS.gene65623:CDS 55.0%
GATTGCTGGGGCATACTGAC+AGG + chr8.2:38001835-38001854 MS.gene65623:intron 55.0%
GCCGCTGAAACAAGAGTGGA+AGG + chr8.2:38001737-38001756 MS.gene65623:intron 55.0%
GGTAAATCCGCGCTCAAGGA+AGG + chr8.2:38002185-38002204 MS.gene65623:CDS 55.0%
GTGCTGGAACGTATCAACCC+AGG + chr8.2:38001522-38001541 MS.gene65623:intron 55.0%
GTTGGAACTGCCGAGGCTTA+GGG + chr8.2:38002554-38002573 MS.gene65623:intron 55.0%
TCATTCAGTTATCCCCCGGC+TGG + chr8.2:38003344-38003363 MS.gene65623:intron 55.0%
TCTACTGAAACTGGGGTGGG+AGG + chr8.2:38002401-38002420 MS.gene65623:intron 55.0%
TGCCCTGTCTCAGACTTCAC+CGG - chr8.2:38001978-38001997 None:intergenic 55.0%
TGCTGCGAAGAGTTCGGAGA+AGG + chr8.2:38002058-38002077 MS.gene65623:CDS 55.0%
TGTTGGAACTGCCGAGGCTT+AGG + chr8.2:38002553-38002572 MS.gene65623:intron 55.0%
! AAGAGGAAGCGTGGACAGAC+TGG + chr8.2:38002338-38002357 MS.gene65623:CDS 55.0%
! AGGGGTGCACGTTCAAGAGA+AGG + chr8.2:38002982-38003001 MS.gene65623:intron 55.0%
! ATTGGAAGCGCATCAGGTGG+TGG + chr8.2:38002223-38002242 MS.gene65623:CDS 55.0%
! CAAGAGTGGAAGGTACGTGC+TGG + chr8.2:38001747-38001766 MS.gene65623:intron 55.0%
! CCTATTGGAAGCGCATCAGG+TGG + chr8.2:38002220-38002239 MS.gene65623:CDS 55.0%
! CTCCTTTGACAGGGTGCCAA+AGG - chr8.2:38001392-38001411 None:intergenic 55.0%
! GATGATGGCGTGAGAGGTTG+GGG + chr8.2:38001888-38001907 MS.gene65623:CDS 55.0%
! GCGCTGATGATCTCAGTTCC+TGG + chr8.2:38003373-38003392 MS.gene65623:intron 55.0%
! TCATTTTCCTCGGTGGGCCT+GGG - chr8.2:38002525-38002544 None:intergenic 55.0%
!! ATTGGTGATGCAGCCACTGG+AGG - chr8.2:38002005-38002024 None:intergenic 55.0%
!! GCTATTGGTGATGCAGCCAC+TGG - chr8.2:38002008-38002027 None:intergenic 55.0%
!! GTGGTGGATTGGCACTCTAC+TGG + chr8.2:38002734-38002753 MS.gene65623:intron 55.0%
ACGCCGCTGCATGAAGCCAA+AGG - chr8.2:38001610-38001629 None:intergenic 60.0%
ACGTGCTGGACGTGGTGACT+GGG + chr8.2:38001761-38001780 MS.gene65623:intron 60.0%
AGGCTGCTGATCCTGCTGGT+TGG + chr8.2:38002144-38002163 MS.gene65623:CDS 60.0%
AGTGACGATTCTGCAGGCGC+CGG + chr8.2:38001410-38001429 MS.gene65623:intron 60.0%
CAATCTCGAGGGCCTCACTC+TGG + chr8.2:38001236-38001255 MS.gene65623:CDS 60.0%
CACGCAGGTTCCCTAAGCCT+CGG - chr8.2:38002567-38002586 None:intergenic 60.0%
CAGCAGGATCAGCAGCCTTC+AGG - chr8.2:38002142-38002161 None:intergenic 60.0%
CAGGATCAGCAGCCTTCAGG+CGG - chr8.2:38002139-38002158 None:intergenic 60.0%
CCGTGAAGTGGAGGAACGGT+TGG + chr8.2:38003107-38003126 MS.gene65623:intron 60.0%
CCGTGGTAAATCCGCGCTCA+AGG + chr8.2:38002181-38002200 MS.gene65623:CDS 60.0%
CGCCGCTGCATGAAGCCAAA+GGG - chr8.2:38001609-38001628 None:intergenic 60.0%
CGCCTTTGGCACCCTGTCAA+AGG + chr8.2:38001387-38001406 MS.gene65623:intron 60.0%
CTGAAGGCTGCTGATCCTGC+TGG + chr8.2:38002140-38002159 MS.gene65623:CDS 60.0%
GGTGCGATTCGCCTACCTGA+AGG + chr8.2:38001353-38001372 MS.gene65623:CDS 60.0%
TACGTGCTGGACGTGGTGAC+TGG + chr8.2:38001760-38001779 MS.gene65623:intron 60.0%
TCAGCGCACAATTCCAGCCG+GGG - chr8.2:38003360-38003379 None:intergenic 60.0%
TGCCAACCGCTCCTTCAGGT+AGG - chr8.2:38001367-38001386 None:intergenic 60.0%
TTCCGGAAAGGGGTCGTCGA+CGG + chr8.2:38002863-38002882 MS.gene65623:intron 60.0%
! CTCATTTTCCTCGGTGGGCC+TGG - chr8.2:38002526-38002545 None:intergenic 60.0%
! GAAACTGGGGTGGGAGGTGT+AGG + chr8.2:38002407-38002426 MS.gene65623:intron 60.0%
! GAAGCGCATCAGGTGGTGGT+GGG + chr8.2:38002227-38002246 MS.gene65623:CDS 60.0%
! GAGGGCCTCACTCTGGAAGA+TGG + chr8.2:38001243-38001262 MS.gene65623:CDS 60.0%
! GGCCTCACTCTGGAAGATGG+TGG + chr8.2:38001246-38001265 MS.gene65623:CDS 60.0%
! GGGTACTGTCCGTGAAGTGG+AGG + chr8.2:38003098-38003117 MS.gene65623:intron 60.0%
! TGGAAGGTACGTGCTGGACG+TGG + chr8.2:38001753-38001772 MS.gene65623:intron 60.0%
!! AACGCTTGGGCGTTGGAACC+TGG + chr8.2:38003279-38003298 MS.gene65623:intron 60.0%
AACGGTGGGTCCTGAGTCCC+AGG + chr8.2:38002505-38002524 MS.gene65623:intron 65.0%
AGGAGCGGTTGGCACGCCTT+TGG + chr8.2:38001373-38001392 MS.gene65623:intron 65.0%
CAGCGCACAATTCCAGCCGG+GGG - chr8.2:38003359-38003378 None:intergenic 65.0%
CGCCTACCTGAAGGAGCGGT+TGG + chr8.2:38001362-38001381 MS.gene65623:intron 65.0%
GACAGGGCAAGCTCCTCCAG+TGG + chr8.2:38001989-38002008 MS.gene65623:CDS 65.0%
GGCTTCATGCAGCGGCGTGT+TGG + chr8.2:38001612-38001631 MS.gene65623:intron 65.0%
TCCCGTCGACGACCCCTTTC+CGG - chr8.2:38002868-38002887 None:intergenic 65.0%
TCCGGAAAGGGGTCGTCGAC+GGG + chr8.2:38002864-38002883 MS.gene65623:intron 65.0%
! GGAAGCGCATCAGGTGGTGG+TGG + chr8.2:38002226-38002245 MS.gene65623:CDS 65.0%
!! ACGATTCTGCAGGCGCCGGA+AGG + chr8.2:38001414-38001433 MS.gene65623:intron 65.0%
!! CGATTCTGCAGGCGCCGGAA+GGG + chr8.2:38001415-38001434 MS.gene65623:intron 65.0%
GGCGTGCCAACCGCTCCTTC+AGG - chr8.2:38001371-38001390 None:intergenic 70.0%
GGGGTCGTCGACGGGAAGCA+TGG + chr8.2:38002872-38002891 MS.gene65623:intron 70.0%
CCTCGGTGGGCCTGGGACTC+AGG - chr8.2:38002518-38002537 None:intergenic 75.0%
CCTGAGTCCCAGGCCCACCG+AGG + chr8.2:38002515-38002534 MS.gene65623:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 38001228 38003498 38001228 ID=MS.gene65623
chr8.2 mRNA 38001228 38003498 38001228 ID=MS.gene65623.t1;Parent=MS.gene65623
chr8.2 exon 38001228 38001379 38001228 ID=MS.gene65623.t1.exon1;Parent=MS.gene65623.t1
chr8.2 CDS 38001228 38001379 38001228 ID=cds.MS.gene65623.t1;Parent=MS.gene65623.t1
chr8.2 exon 38001857 38002415 38001857 ID=MS.gene65623.t1.exon2;Parent=MS.gene65623.t1
chr8.2 CDS 38001857 38002415 38001857 ID=cds.MS.gene65623.t1;Parent=MS.gene65623.t1
chr8.2 exon 38003472 38003498 38003472 ID=MS.gene65623.t1.exon3;Parent=MS.gene65623.t1
chr8.2 CDS 38003472 38003498 38003472 ID=cds.MS.gene65623.t1;Parent=MS.gene65623.t1
Gene Sequence

>MS.gene65623

ATGGAGTTCAATCTCGAGGGCCTCACTCTGGAAGATGGTGGTATGACTCTGAATTTGGATACTACTGATGCTGCTGTCACTATTTTGGATCAATGCTTGATAGGCCGTGTGCTAACTGATAGGGAGGTGCGATTCGCCTACCTGAAGGAGCGGTTGGCACGCCTTTGGCACCCTGTCAAAGGAGTGACGATTCTGCAGGCGCCGGAAGGGCGATTTCTGTTCCAGTTTAATCACAAGAAGGATGCTGAAAATGCTATGAATGCCGGTCCGTGGATGTATGACAACTGTAACTTGGTGCTGGAACGTATCAACCCAGGTATGGTCCCAAAGGATGTTGAGCTGAATCACCTGGACATGTGGATTCAGGTTCACAATTTACCCTTTGGCTTCATGCAGCGGCGTGTTGGAGATGCTATTGGTGCCTACCTTGGTGAATTGAAGGAGTATGATGAGAAGAATACACCACATAGTGAATTTATGAAGTTGAAAGTCAGAATTGATATCACAAAGCCGCTGAAACAAGAGTGGAAGGTACGTGCTGGACGTGGTGACTGGGTCTCTGTTGTGTTCAAGTACGAAAGGTTGGGGACTTTCTGTTATGCTTGTGGATTGCTGGGGCATACTGACAGGTCCTGTGAGCAATTGTTTGAGAATGATATAGATGATGGCGTGAGAGGTTGGGGAGATTACCTCAAGACAACTTCTCGCAAACTTGGTACTGCTGCTAACAACAAATGGTTGCGGAATCCGGTGAAGTCTGAGACAGGGCAAGCTCCTCCAGTGGCTGCATCACCAATAGCTGCGAATTCTGATGAAGCTTCAGGTAGCAATGCTGCGAAGAGTTCGGAGAAGGCAATGTTGAATGGAAGAATGACTGTTGTTCGGTCCGAGATTACGACTCTTAAGAACCGCCTGAAGGCTGCTGATCCTGCTGGTTGGAAAGCTTTTGAGGACCGTGGTAAATCCGCGCTCAAGGAAGGTAATGACATGAACCTATTGGAAGCGCATCAGGTGGTGGTGGGACTTACTGCTCCTACTGATGAGGTCCTAGCACTGGAGTATGTTGACTCTGCTGGTATAGTGGACACAGAAGCAGGGTCTGATCTGAAGAAGAGGAAGCGTGGACAGACTGGAGCTCCTCCTATTGATGCTGTTGCTGCACCTATGCAAGTGTCTACTGAAACTGGGGTGGGAGGTGTAGGTATAGCTGCTGTTAGTGGGGGGGAAACTAGTGATTTGAGAATGTTTACTAATCCTGTGTTTGATGAAACTGATGTAACGGTGGGTCCTGAGTCCCAGGCCCACCGAGGAAAATGAGTCTGTTGTGTTGGAACTGCCGAGGCTTAGGGAACCTGCGTGTAGTTCCAAAACTGAAGTACCTCGTTCGGTATTATAAACCGGATGTTTTCTTTCTTTGCGAGACTTTAGTCTCTAATAATAAAAGTGAAGATCTTCGTTATGTCCTTGGTTTTGATTGTTGTTTTGCTGTTAGTAGTTCTGGTAGAAGTGGTGGATTGGCACTCTACTGGAATAATTCATTTAATTGTTCACTTGTTAATTATTCTTGTAATCATATTTCTATCCAAATTATTGATCCAGATAAAGGTCCTTGGCAATTCACAGGTTATTATGGGTTTCCGGAAAGGGGTCGTCGACGGGAAGCATGGAATTTTCTCCGAGGTTTAGCCCGGGATGTATCACTACCATGGTGCATTGGTGGTGACTTTAATGATATTTTGTTTGAACATGAGAAAAGGGGTGCACGTTCAAGAGAAGGATGGCTGATCAATGGGTTTCGTCAAGCAGTTTCGGACACCGAGCTTGTTGATGTCCCTATGATTGGTTATCCGTTTACATGGTTCAAGAGTATGGGTACTGTCCGTGAAGTGGAGGAACGGTTGGACAGAGTATTGGTTCGAGATAGTTGGTTTTCTTTGTTTCCGAATGCACAGGTTGAGAATTTAGTAGCGCCGGCTTCTGATCACCATCCCATTCTGCTCTCCTTGGTACCGCAAGTTAGAAGTGGCTCAAGGCTGAAATTTAAATACGAAAACGCTTGGGCGTTGGAACCTGGTTTTCATGACATGGTAGCAGATCTGTGGCATTATCATCATGGTCATTCAGTTATCCCCCGGCTGGAATTGTGCGCTGATGATCTCAGTTCCTGGAGTCAGACACATTGTAACAAATTAAGACAGGAGATTGCAGAATGTCGCAAAACTCTTAGTGTGTGTCGTGATCTAGGCATTGCGTGTGACCCACACCATTTAG
Protein sequence

>MS.gene65623.t1

MEFNLEGLTLEDGGMTLNLDTTDAAVTILDQCLIGRVLTDREVRFAYLKERSCEQLFENDIDDGVRGWGDYLKTTSRKLGTAANNKWLRNPVKSETGQAPPVAASPIAANSDEASGSNAAKSSEKAMLNGRMTVVRSEITTLKNRLKAADPAGWKAFEDRGKSALKEGNDMNLLEAHQVVVGLTAPTDEVLALEYVDSAGIVDTEAGSDLKKRKRGQTGAPPIDAVAAPMQVSTETGALRVTHTI