AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63743

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63743.t1 MTR_1g036720 41.509 106 60 1 1 106 4 107 3.07e-22 89.0
MS.gene63743.t1 MTR_0010s0370 42.241 116 61 2 1 114 4 115 2.43e-20 86.3
MS.gene63743.t1 MTR_0010s0350 42.241 116 61 2 1 114 4 115 2.43e-20 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACAGTTGTTAATCAAAT+AGG 0.201663 2.1:+23489179 MS.gene63743:CDS
GATGGGCACACTCTGGTTCT+AGG 0.203405 2.1:+23488591 MS.gene63743:CDS
GTTCTTTCATCCTCGTGATA+TGG 0.266174 2.1:+23488536 MS.gene63743:CDS
ATGATGATGGTACACGTTTA+TGG 0.293979 2.1:+23488973 MS.gene63743:CDS
TCTTGAGGGTTCCTATGTTC+TGG 0.324397 2.1:+23488389 MS.gene63743:CDS
GCGAGGATGGTGGTCCAATC+AGG 0.325917 2.1:+23489060 MS.gene63743:CDS
CCGACATGGTTGATGAGAAT+TGG 0.350656 2.1:+23490283 MS.gene63743:CDS
CAGACCGGTTGCATGAATTC+AGG 0.359344 2.1:-23488468 None:intergenic
GCTTCTGCTGATTCTCTTGA+GGG 0.360923 2.1:+23488375 MS.gene63743:CDS
AATCAGCAGAAGCTTGTCTT+TGG 0.367306 2.1:-23488365 None:intergenic
ACTACATCTGTGCCTGTTAC+GGG 0.373043 2.1:+23489128 MS.gene63743:CDS
CATCATCAGTATTCGCATAA+AGG 0.374801 2.1:-23488956 None:intergenic
GGTTGAGGAAATAAAGTTGC+AGG 0.379209 2.1:-23489236 None:intergenic
CAACAGTTGTTAATCAAATA+GGG 0.379587 2.1:+23489180 MS.gene63743:CDS
TGAGTGCTCCCACCCGTAAC+AGG 0.380805 2.1:-23489140 None:intergenic
GTTAATTTAGCCGGTGGTTG+AGG 0.401264 2.1:-23489251 None:intergenic
CCCATGGAACATCTCTCAAT+TGG 0.401976 2.1:+23488309 None:intergenic
CGACCTCCTTGATAGTCATT+TGG 0.409105 2.1:-23488491 None:intergenic
TACTACATCTGTGCCTGTTA+CGG 0.421518 2.1:+23489127 MS.gene63743:CDS
AGGCACAGATGTAGTATTCA+TGG 0.427302 2.1:-23489120 None:intergenic
TTAATTGAGGAAGAATTAGA+TGG 0.427360 2.1:+23489311 MS.gene63743:CDS
GAAGCTTGTCTTTGGTCAAT+CGG 0.440725 2.1:-23488357 None:intergenic
ATGGGCACACTCTGGTTCTA+GGG 0.441651 2.1:+23488592 MS.gene63743:CDS
AGCTTCTGCTGATTCTCTTG+AGG 0.451498 2.1:+23488374 MS.gene63743:CDS
ACCATCCTCGCGCAGCCACT+TGG 0.451864 2.1:-23489048 None:intergenic
AATTCAGATTCATTCACAAC+AGG 0.460614 2.1:-23489290 None:intergenic
GATGCCTGAATTCATGCAAC+CGG 0.463779 2.1:+23488464 MS.gene63743:CDS
TATCACGAGGATGAAAGAAC+TGG 0.477239 2.1:-23488533 None:intergenic
AACTTTATTTCCTCAACCAC+CGG 0.484220 2.1:+23489241 MS.gene63743:CDS
GGCTGCTGCACCATCACATA+AGG 0.484436 2.1:+23489082 MS.gene63743:CDS
CCTATTTGATTAACAACTGT+TGG 0.492070 2.1:-23489179 None:intergenic
GAGGGTTCCTATGTTCTGGT+TGG 0.495546 2.1:+23488393 MS.gene63743:CDS
TTGATAGTCATTTGGCAGAC+CGG 0.497718 2.1:-23488483 None:intergenic
GTTGTTCCTGCCTTATGTGA+TGG 0.502636 2.1:-23489092 None:intergenic
TGATCCAAGTTTCTCCGACA+TGG 0.504866 2.1:+23490269 MS.gene63743:intron
GCATCTTTCCTTTCATCGTG+TGG 0.506968 2.1:-23488446 None:intergenic
GATGGTGCAGCAGCCCTGAT+TGG 0.509185 2.1:-23489074 None:intergenic
ATTCGATTCCACACGATGAA+AGG 0.511904 2.1:+23488438 MS.gene63743:CDS
ATGGAACATCTCTCAATTGG+AGG 0.516295 2.1:+23488312 MS.gene63743:CDS
TCCAATTGAGAGATGTTCCA+TGG 0.522615 2.1:-23488310 None:intergenic
GAACTGGAATAGAAACAGAT+TGG 0.524109 2.1:-23488517 None:intergenic
TCCAAGTGGCTGCGCGAGGA+TGG 0.532648 2.1:+23489047 MS.gene63743:CDS
CAAGTATTGTGTTGACGACA+TGG 0.538229 2.1:+23490341 MS.gene63743:CDS
CGAGGATGGTGGTCCAATCA+GGG 0.539122 2.1:+23489061 MS.gene63743:CDS
AAGTGGCTGCGCGAGGATGG+TGG 0.550452 2.1:+23489050 MS.gene63743:CDS
CCAATTGAGAGATGTTCCAT+GGG 0.551382 2.1:-23488309 None:intergenic
GAAACGTCCAACCAGAACAT+AGG 0.560594 2.1:-23488400 None:intergenic
ACATCTGTGCCTGTTACGGG+TGG 0.565738 2.1:+23489131 MS.gene63743:CDS
GAATGAATCTGAATTAATTG+AGG 0.568291 2.1:+23489298 MS.gene63743:CDS
GTTTGAGAATGCTTCAGCCA+TGG 0.570314 2.1:-23489213 None:intergenic
GCTGCCGAAGTTAATTTAGC+CGG 0.578405 2.1:-23489260 None:intergenic
CGTGCATTAACAAAGAGGAA+TGG 0.587216 2.1:+23489008 MS.gene63743:CDS
GTTGAGGAAATAAAGTTGCA+GGG 0.595423 2.1:-23489235 None:intergenic
AACAGTTGTTAATCAAATAG+GGG 0.601891 2.1:+23489181 MS.gene63743:CDS
AATAGGGGCTCATATATCCA+TGG 0.603254 2.1:+23489196 MS.gene63743:CDS
GCCGAAGTTAATTTAGCCGG+TGG 0.610975 2.1:-23489257 None:intergenic
TTAACAAAGAGGAATGGAAG+TGG 0.611467 2.1:+23489014 MS.gene63743:CDS
TATGCGAATACTGATGATGA+TGG 0.619477 2.1:+23488960 MS.gene63743:CDS
GCTGCACCATCACATAAGGC+AGG 0.621807 2.1:+23489086 MS.gene63743:CDS
ACTTACGTGCATTAACAAAG+AGG 0.624715 2.1:+23489003 MS.gene63743:CDS
TACATCCAAGTGGCTGCGCG+AGG 0.629605 2.1:+23489043 MS.gene63743:CDS
ACCACCGGCTAAATTAACTT+CGG 0.631740 2.1:+23489256 MS.gene63743:CDS
CCAATTCTCATCAACCATGT+CGG 0.632932 2.1:-23490283 None:intergenic
CATCTGTGCCTGTTACGGGT+GGG 0.634505 2.1:+23489132 MS.gene63743:CDS
ACAAAGAGGAATGGAAGTGG+AGG 0.634722 2.1:+23489017 MS.gene63743:CDS
GGTCTGCCAAATGACTATCA+AGG 0.650531 2.1:+23488485 MS.gene63743:CDS
GCTCCTCAAAGAAAAGAACA+AGG 0.694250 2.1:+23489333 MS.gene63743:CDS
AGAACGTGTTCCATATCACG+AGG 0.700645 2.1:-23488546 None:intergenic
GTGTGCCCATCAAAAGACCA+CGG 0.715824 2.1:-23488579 None:intergenic
GTGGAGGTTCTACATCCAAG+TGG 0.722594 2.1:+23489033 MS.gene63743:CDS
ATCGAATATGACGATCTGTG+AGG 0.723346 2.1:-23488422 None:intergenic
CTGCCAAATGACTATCAAGG+AGG 0.746944 2.1:+23488488 MS.gene63743:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATAAGGAAAAAAATATC+CGG - chr2.1:23489604-23489623 None:intergenic 20.0%
!!! CTTAGAGTGAATATAATTTT+CGG - chr2.1:23490217-23490236 None:intergenic 20.0%
!!! TTAGAGTGAATATAATTTTC+GGG - chr2.1:23490216-23490235 None:intergenic 20.0%
! AACAGTTGTTAATCAAATAG+GGG + chr2.1:23489181-23489200 MS.gene63743:CDS 25.0%
! ATGATTTCCAAATAATAGCA+AGG - chr2.1:23489720-23489739 None:intergenic 25.0%
! CAACAGTTGTTAATCAAATA+GGG + chr2.1:23489180-23489199 MS.gene63743:CDS 25.0%
! CAACTTTATTGGAGAATATA+TGG + chr2.1:23488731-23488750 MS.gene63743:intron 25.0%
! GAATGAATCTGAATTAATTG+AGG + chr2.1:23489298-23489317 MS.gene63743:CDS 25.0%
! TGAATTGATATCCTTCTAAT+GGG - chr2.1:23490038-23490057 None:intergenic 25.0%
! TTAATTGAGGAAGAATTAGA+TGG + chr2.1:23489311-23489330 MS.gene63743:CDS 25.0%
!! ACGAAATGAGTATTTTATGT+TGG + chr2.1:23489512-23489531 MS.gene63743:intron 25.0%
!! TTTTGCATGATGTTATTAGA+TGG - chr2.1:23490318-23490337 None:intergenic 25.0%
!!! TAGATAAGCAGCATTTTTTA+TGG + chr2.1:23489462-23489481 MS.gene63743:intron 25.0%
AAACAACTTGGCAACTTTAT+TGG + chr2.1:23488720-23488739 MS.gene63743:intron 30.0%
AATGCGACAAAGTTTCAATA+AGG - chr2.1:23489618-23489637 None:intergenic 30.0%
AATTCAGATTCATTCACAAC+AGG - chr2.1:23489293-23489312 None:intergenic 30.0%
CAAACCACAGTAATAACAAA+AGG - chr2.1:23488907-23488926 None:intergenic 30.0%
CATAAAATACTCATTTCGTC+CGG - chr2.1:23489511-23489530 None:intergenic 30.0%
CCAACAGTTGTTAATCAAAT+AGG + chr2.1:23489179-23489198 MS.gene63743:CDS 30.0%
CCTATTTGATTAACAACTGT+TGG - chr2.1:23489182-23489201 None:intergenic 30.0%
GTGAATTGATATCCTTCTAA+TGG - chr2.1:23490039-23490058 None:intergenic 30.0%
GTTTCAAATATGAGAGACTT+GGG + chr2.1:23488880-23488899 MS.gene63743:intron 30.0%
TAGAAGGATATCAATTCACA+TGG + chr2.1:23490040-23490059 MS.gene63743:intron 30.0%
TAGTAGAATAGGTGAATGAT+CGG - chr2.1:23490184-23490203 None:intergenic 30.0%
TGATTTAGAGGAACAGATTT+AGG - chr2.1:23488639-23488658 None:intergenic 30.0%
! TATTTTACAGTTGATCGAGA+AGG + chr2.1:23489682-23489701 MS.gene63743:intron 30.0%
! TCTAAATCACATACCCTTTT+GGG + chr2.1:23488650-23488669 MS.gene63743:intron 30.0%
!! AGAGATGTTTGTTATATGCT+TGG + chr2.1:23489649-23489668 MS.gene63743:intron 30.0%
!! TGTTATTACTGTGGTTTGTT+AGG + chr2.1:23488909-23488928 MS.gene63743:intron 30.0%
AAATCACCTATGATACACCA+AGG - chr2.1:23489910-23489929 None:intergenic 35.0%
AAGAAGATTAGGACTAAAGC+TGG + chr2.1:23488843-23488862 MS.gene63743:intron 35.0%
AATCACCTATGATACACCAA+GGG - chr2.1:23489909-23489928 None:intergenic 35.0%
AATCTTCTTAAATCTCGCCA+AGG - chr2.1:23488832-23488851 None:intergenic 35.0%
ACTTACGTGCATTAACAAAG+AGG + chr2.1:23489003-23489022 MS.gene63743:CDS 35.0%
AGATTGATAAGTTGTGCGTT+CGG - chr2.1:23490147-23490166 None:intergenic 35.0%
ATCTTCTTAAATCTCGCCAA+GGG - chr2.1:23488831-23488850 None:intergenic 35.0%
ATGATCCAGTTAGAGGTAAT+TGG + chr2.1:23489794-23489813 MS.gene63743:intron 35.0%
ATGGAACACGTTCTCAAAAA+AGG + chr2.1:23488555-23488574 MS.gene63743:CDS 35.0%
ATTACTGCACGTAAACAACT+TGG + chr2.1:23488708-23488727 MS.gene63743:intron 35.0%
ATTGATATCCTTCTAATGGG+AGG - chr2.1:23490035-23490054 None:intergenic 35.0%
CAAGTAAATGATCCAGTTAG+AGG + chr2.1:23489787-23489806 MS.gene63743:intron 35.0%
CATCATCAGTATTCGCATAA+AGG - chr2.1:23488959-23488978 None:intergenic 35.0%
CCTTATTCGTGTCTATAAAC+CGG + chr2.1:23489582-23489601 MS.gene63743:intron 35.0%
CGTTTCAAATATGAGAGACT+TGG + chr2.1:23488879-23488898 MS.gene63743:intron 35.0%
GAACTGGAATAGAAACAGAT+TGG - chr2.1:23488520-23488539 None:intergenic 35.0%
GAATACGCATATATGAAGCT+AGG - chr2.1:23488785-23488804 None:intergenic 35.0%
GCTGACACTACAAATCTTAA+TGG + chr2.1:23489380-23489399 MS.gene63743:intron 35.0%
GTTGAGGAAATAAAGTTGCA+GGG - chr2.1:23489238-23489257 None:intergenic 35.0%
TAATTGTCATACAACCGACT+GGG - chr2.1:23488692-23488711 None:intergenic 35.0%
TACAATCAATGACAGCTGAT+CGG - chr2.1:23490002-23490021 None:intergenic 35.0%
TGCTGCAAAATTCATCAGTA+TGG - chr2.1:23488935-23488954 None:intergenic 35.0%
TGGCGAGATTTAAGAAGATT+AGG + chr2.1:23488832-23488851 MS.gene63743:intron 35.0%
TTAACAAAGAGGAATGGAAG+TGG + chr2.1:23489014-23489033 MS.gene63743:CDS 35.0%
TTCACCTATTCTACTACATC+TGG + chr2.1:23490188-23490207 MS.gene63743:intron 35.0%
! AACTTTATTTCCTCAACCAC+CGG + chr2.1:23489241-23489260 MS.gene63743:CDS 35.0%
! CAAAAGGGTATGTGATTTAG+AGG - chr2.1:23488651-23488670 None:intergenic 35.0%
! CTCTAAATCACATACCCTTT+TGG + chr2.1:23488649-23488668 MS.gene63743:intron 35.0%
! TATGCGAATACTGATGATGA+TGG + chr2.1:23488960-23488979 MS.gene63743:CDS 35.0%
!! ATGATGATGGTACACGTTTA+TGG + chr2.1:23488973-23488992 MS.gene63743:CDS 35.0%
!! CGAATAAGGTCTTTAAGACT+AGG - chr2.1:23489571-23489590 None:intergenic 35.0%
!! GCTTTCGCTTTGAAAATTCA+TGG + chr2.1:23490241-23490260 MS.gene63743:intron 35.0%
!!! CATTTTTTATGGACAGAGTC+TGG + chr2.1:23489473-23489492 MS.gene63743:intron 35.0%
AATAGGGGCTCATATATCCA+TGG + chr2.1:23489196-23489215 MS.gene63743:CDS 40.0%
AATAGGTGAATGATCGGAAG+TGG - chr2.1:23490178-23490197 None:intergenic 40.0%
AATCAGCAGAAGCTTGTCTT+TGG - chr2.1:23488368-23488387 None:intergenic 40.0%
AATCCATTATAGAGCCAGTG+AGG - chr2.1:23489979-23489998 None:intergenic 40.0%
AGGCACAGATGTAGTATTCA+TGG - chr2.1:23489123-23489142 None:intergenic 40.0%
AGTAACCCCAGAAAGAAGTT+AGG - chr2.1:23489822-23489841 None:intergenic 40.0%
AGTCTTTCTTCAACAACACG+TGG - chr2.1:23490084-23490103 None:intergenic 40.0%
ATCGAATATGACGATCTGTG+AGG - chr2.1:23488425-23488444 None:intergenic 40.0%
ATGGAACATCTCTCAATTGG+AGG + chr2.1:23488312-23488331 MS.gene63743:CDS 40.0%
ATTCGATTCCACACGATGAA+AGG + chr2.1:23488438-23488457 MS.gene63743:CDS 40.0%
CCAATTCTCATCAACCATGT+CGG - chr2.1:23490286-23490305 None:intergenic 40.0%
CCGGTTTATAGACACGAATA+AGG - chr2.1:23489585-23489604 None:intergenic 40.0%
CGTGCATTAACAAAGAGGAA+TGG + chr2.1:23489008-23489027 MS.gene63743:CDS 40.0%
GAAGCTTGTCTTTGGTCAAT+CGG - chr2.1:23488360-23488379 None:intergenic 40.0%
GATTGATAAGTTGTGCGTTC+GGG - chr2.1:23490146-23490165 None:intergenic 40.0%
GCTCCTCAAAGAAAAGAACA+AGG + chr2.1:23489333-23489352 MS.gene63743:CDS 40.0%
GGTTCCAGATGTAGTAGAAT+AGG - chr2.1:23490195-23490214 None:intergenic 40.0%
GGTTGAGGAAATAAAGTTGC+AGG - chr2.1:23489239-23489258 None:intergenic 40.0%
GTAATTGTCATACAACCGAC+TGG - chr2.1:23488693-23488712 None:intergenic 40.0%
GTCTTTCTTCAACAACACGT+GGG - chr2.1:23490083-23490102 None:intergenic 40.0%
GTTCTTTCATCCTCGTGATA+TGG + chr2.1:23488536-23488555 MS.gene63743:CDS 40.0%
TAAATCTCGCCAAGGGTTTA+CGG - chr2.1:23488824-23488843 None:intergenic 40.0%
TACTACATCTGTGCCTGTTA+CGG + chr2.1:23489127-23489146 MS.gene63743:CDS 40.0%
TATCACGAGGATGAAAGAAC+TGG - chr2.1:23488536-23488555 None:intergenic 40.0%
TCACATGGTTCAAAAGCCTT+GGG + chr2.1:23490055-23490074 MS.gene63743:intron 40.0%
TCATGGATTTGCACCCAAAA+GGG - chr2.1:23488666-23488685 None:intergenic 40.0%
TCTTTCTTCAACAACACGTG+GGG - chr2.1:23490082-23490101 None:intergenic 40.0%
TTAAGACTAGGAACTGCCTT+AGG - chr2.1:23489559-23489578 None:intergenic 40.0%
TTCACATGGTTCAAAAGCCT+TGG + chr2.1:23490054-23490073 MS.gene63743:intron 40.0%
! ACCACCGGCTAAATTAACTT+CGG + chr2.1:23489256-23489275 MS.gene63743:CDS 40.0%
! ATTTTATGTTGGAACTGCCG+AGG + chr2.1:23489523-23489542 MS.gene63743:intron 40.0%
! CAAGTATTGTGTTGACGACA+TGG + chr2.1:23490341-23490360 MS.gene63743:CDS 40.0%
! GCACCTTGTTCTTTTCTTTG+AGG - chr2.1:23489339-23489358 None:intergenic 40.0%
! TTGATAGTCATTTGGCAGAC+CGG - chr2.1:23488486-23488505 None:intergenic 40.0%
!! GTTCTAGGGTTTCTACAACA+AGG + chr2.1:23488606-23488625 MS.gene63743:intron 40.0%
!!! GGGTCCTTTTGTTATTACTG+TGG + chr2.1:23488900-23488919 MS.gene63743:intron 40.0%
AAATACTCATTTCGTCCGGC+AGG - chr2.1:23489507-23489526 None:intergenic 45.0%
ACAAAGAGGAATGGAAGTGG+AGG + chr2.1:23489017-23489036 MS.gene63743:CDS 45.0%
ACGTTCTCAAAAAAGGACCG+TGG + chr2.1:23488562-23488581 MS.gene63743:CDS 45.0%
ACTACATCTGTGCCTGTTAC+GGG + chr2.1:23489128-23489147 MS.gene63743:CDS 45.0%
AGAACGTGTTCCATATCACG+AGG - chr2.1:23488549-23488568 None:intergenic 45.0%
ATTGGCGCCTAACTTCTTTC+TGG + chr2.1:23489812-23489831 MS.gene63743:intron 45.0%
CTCATGGATTTGCACCCAAA+AGG - chr2.1:23488667-23488686 None:intergenic 45.0%
CTCGTTTGTCATCGCTTGTA+AGG - chr2.1:23489942-23489961 None:intergenic 45.0%
CTGCCAAATGACTATCAAGG+AGG + chr2.1:23488488-23488507 MS.gene63743:CDS 45.0%
CTTACAAGCGATGACAAACG+AGG + chr2.1:23489940-23489959 MS.gene63743:intron 45.0%
GAAACGTCCAACCAGAACAT+AGG - chr2.1:23488403-23488422 None:intergenic 45.0%
GATGCCTGAATTCATGCAAC+CGG + chr2.1:23488464-23488483 MS.gene63743:CDS 45.0%
GATTGATGTCCGTAAACCCT+TGG + chr2.1:23488812-23488831 MS.gene63743:intron 45.0%
GCATCTTTCCTTTCATCGTG+TGG - chr2.1:23488449-23488468 None:intergenic 45.0%
GCGCTTTCTATACATCCTCA+TGG + chr2.1:23490109-23490128 MS.gene63743:intron 45.0%
GCTGCCGAAGTTAATTTAGC+CGG - chr2.1:23489263-23489282 None:intergenic 45.0%
GGTCTGCCAAATGACTATCA+AGG + chr2.1:23488485-23488504 MS.gene63743:CDS 45.0%
GTTAATTTAGCCGGTGGTTG+AGG - chr2.1:23489254-23489273 None:intergenic 45.0%
GTTGTTCCTGCCTTATGTGA+TGG - chr2.1:23489095-23489114 None:intergenic 45.0%
GTTTGAGAATGCTTCAGCCA+TGG - chr2.1:23489216-23489235 None:intergenic 45.0%
GTTTGTCATCGCTTGTAAGG+AGG - chr2.1:23489939-23489958 None:intergenic 45.0%
TCAACCATGTCGGAGAAACT+TGG - chr2.1:23490276-23490295 None:intergenic 45.0%
TCTTGAGGGTTCCTATGTTC+TGG + chr2.1:23488389-23488408 MS.gene63743:CDS 45.0%
TGATCCAAGTTTCTCCGACA+TGG + chr2.1:23490269-23490288 MS.gene63743:intron 45.0%
TGTCGGAGAAACTTGGATCA+AGG - chr2.1:23490269-23490288 None:intergenic 45.0%
TTGCATGACCTCCCATTAGA+AGG + chr2.1:23490024-23490043 MS.gene63743:intron 45.0%
TTGGCGCCTAACTTCTTTCT+GGG + chr2.1:23489813-23489832 MS.gene63743:intron 45.0%
! AAAGGACCGTGGTCTTTTGA+TGG + chr2.1:23488573-23488592 MS.gene63743:CDS 45.0%
! AACGACCCTTGGTGTATCAT+AGG + chr2.1:23489901-23489920 MS.gene63743:intron 45.0%
! AAGGACCGTGGTCTTTTGAT+GGG + chr2.1:23488574-23488593 MS.gene63743:CDS 45.0%
! CGACCTCCTTGATAGTCATT+TGG - chr2.1:23488494-23488513 None:intergenic 45.0%
!! AGACCTCACTGGCTCTATAA+TGG + chr2.1:23489973-23489992 MS.gene63743:intron 45.0%
!! AGCTTCTGCTGATTCTCTTG+AGG + chr2.1:23488374-23488393 MS.gene63743:CDS 45.0%
!! CCGACATGGTTGATGAGAAT+TGG + chr2.1:23490283-23490302 MS.gene63743:CDS 45.0%
!! CTTTTGGGTGCAAATCCATG+AGG + chr2.1:23488665-23488684 MS.gene63743:intron 45.0%
!! GCTTCTGCTGATTCTCTTGA+GGG + chr2.1:23488375-23488394 MS.gene63743:CDS 45.0%
!! GTGGTGGCCTTGCTATTATT+TGG + chr2.1:23489710-23489729 MS.gene63743:intron 45.0%
ACAAGCGATGACAAACGAGG+AGG + chr2.1:23489943-23489962 MS.gene63743:intron 50.0%
AGGCGCCAATTACCTCTAAC+TGG - chr2.1:23489802-23489821 None:intergenic 50.0%
AGTGTCAGCTGATGTGCTAC+TGG - chr2.1:23489369-23489388 None:intergenic 50.0%
ATAGAGCCAGTGAGGTCTCT+CGG - chr2.1:23489971-23489990 None:intergenic 50.0%
CAAATCCATGAGGTCCCAGT+CGG + chr2.1:23488675-23488694 MS.gene63743:intron 50.0%
CAGACCGGTTGCATGAATTC+AGG - chr2.1:23488471-23488490 None:intergenic 50.0%
CCAAGGGTCGTTAGACATAG+AGG - chr2.1:23489893-23489912 None:intergenic 50.0%
CCTCTATGTCTAACGACCCT+TGG + chr2.1:23489890-23489909 MS.gene63743:intron 50.0%
CCTGAGACGAAAGCTATGGA+CGG + chr2.1:23489407-23489426 MS.gene63743:intron 50.0%
CTCACCTGAGACGAAAGCTA+TGG + chr2.1:23489403-23489422 MS.gene63743:intron 50.0%
GAGGGTTCCTATGTTCTGGT+TGG + chr2.1:23488393-23488412 MS.gene63743:CDS 50.0%
GCCGAAGTTAATTTAGCCGG+TGG - chr2.1:23489260-23489279 None:intergenic 50.0%
GGGGTTACTCTAACACAGCT+CGG + chr2.1:23489833-23489852 MS.gene63743:intron 50.0%
GGTCGTTAGACATAGAGGCA+AGG - chr2.1:23489888-23489907 None:intergenic 50.0%
GTGGAGGTTCTACATCCAAG+TGG + chr2.1:23489033-23489052 MS.gene63743:CDS 50.0%
GTGTGCCCATCAAAAGACCA+CGG - chr2.1:23488582-23488601 None:intergenic 50.0%
GTTGATCGAGAAGGCTGTAG+TGG + chr2.1:23489691-23489710 MS.gene63743:intron 50.0%
TGGCGCCTAACTTCTTTCTG+GGG + chr2.1:23489814-23489833 MS.gene63743:intron 50.0%
!! ATGGGCACACTCTGGTTCTA+GGG + chr2.1:23488592-23488611 MS.gene63743:CDS 50.0%
!! GTCTTTTGATGGGCACACTC+TGG + chr2.1:23488584-23488603 MS.gene63743:CDS 50.0%
ACATCTGTGCCTGTTACGGG+TGG + chr2.1:23489131-23489150 MS.gene63743:CDS 55.0%
CAGCTCGGCGTAGACAATCA+TGG + chr2.1:23489848-23489867 MS.gene63743:intron 55.0%
CATCTGTGCCTGTTACGGGT+GGG + chr2.1:23489132-23489151 MS.gene63743:CDS 55.0%
CCGTCCATAGCTTTCGTCTC+AGG - chr2.1:23489410-23489429 None:intergenic 55.0%
CGGGAAAAGAGTGAGCCATG+AGG - chr2.1:23490127-23490146 None:intergenic 55.0%
GAACTGCCTTAGGATGTCCC+AGG - chr2.1:23489549-23489568 None:intergenic 55.0%
GATCGAGAAGGCTGTAGTGG+TGG + chr2.1:23489694-23489713 MS.gene63743:intron 55.0%
GCTGCACCATCACATAAGGC+AGG + chr2.1:23489086-23489105 MS.gene63743:CDS 55.0%
GGCTGCTGCACCATCACATA+AGG + chr2.1:23489082-23489101 MS.gene63743:CDS 55.0%
TACAACCGACTGGGACCTCA+TGG - chr2.1:23488683-23488702 None:intergenic 55.0%
! ATGGACAGAGTCTGGTTCCC+AGG + chr2.1:23489481-23489500 MS.gene63743:intron 55.0%
! CGAGGATGGTGGTCCAATCA+GGG + chr2.1:23489061-23489080 MS.gene63743:CDS 55.0%
!! GATGGGCACACTCTGGTTCT+AGG + chr2.1:23488591-23488610 MS.gene63743:CDS 55.0%
ATGTTGGAACTGCCGAGGCC+TGG + chr2.1:23489528-23489547 MS.gene63743:intron 60.0%
CAACAACACGTGGGGTCCCA+AGG - chr2.1:23490074-23490093 None:intergenic 60.0%
CCTTAGGATGTCCCAGGCCT+CGG - chr2.1:23489543-23489562 None:intergenic 60.0%
GAGGATCCGAGAGACCTCAC+TGG + chr2.1:23489962-23489981 MS.gene63743:intron 60.0%
GATGGTGCAGCAGCCCTGAT+TGG - chr2.1:23489077-23489096 None:intergenic 60.0%
TACATCCAAGTGGCTGCGCG+AGG + chr2.1:23489043-23489062 MS.gene63743:CDS 60.0%
TCATTTCGTCCGGCAGGCCT+GGG - chr2.1:23489501-23489520 None:intergenic 60.0%
TGAGTGCTCCCACCCGTAAC+AGG - chr2.1:23489143-23489162 None:intergenic 60.0%
TGTTGGAACTGCCGAGGCCT+GGG + chr2.1:23489529-23489548 MS.gene63743:intron 60.0%
! GCGAGGATGGTGGTCCAATC+AGG + chr2.1:23489060-23489079 MS.gene63743:CDS 60.0%
AAGTGGCTGCGCGAGGATGG+TGG + chr2.1:23489050-23489069 MS.gene63743:CDS 65.0%
ACCATCCTCGCGCAGCCACT+TGG - chr2.1:23489051-23489070 None:intergenic 65.0%
CCGAGGCCTGGGACATCCTA+AGG + chr2.1:23489540-23489559 MS.gene63743:intron 65.0%
CTCATTTCGTCCGGCAGGCC+TGG - chr2.1:23489502-23489521 None:intergenic 65.0%
TCCAAGTGGCTGCGCGAGGA+TGG + chr2.1:23489047-23489066 MS.gene63743:CDS 65.0%
! AGTCTGGTTCCCAGGCCTGC+CGG + chr2.1:23489489-23489508 MS.gene63743:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 23488312 23490371 23488312 ID=MS.gene63743
chr2.1 mRNA 23488312 23490371 23488312 ID=MS.gene63743.t1;Parent=MS.gene63743
chr2.1 exon 23488312 23488613 23488312 ID=MS.gene63743.t1.exon1;Parent=MS.gene63743.t1
chr2.1 CDS 23488312 23488613 23488312 ID=cds.MS.gene63743.t1;Parent=MS.gene63743.t1
chr2.1 exon 23488953 23489354 23488953 ID=MS.gene63743.t1.exon2;Parent=MS.gene63743.t1
chr2.1 CDS 23488953 23489354 23488953 ID=cds.MS.gene63743.t1;Parent=MS.gene63743.t1
chr2.1 exon 23490278 23490371 23490278 ID=MS.gene63743.t1.exon3;Parent=MS.gene63743.t1
chr2.1 CDS 23490278 23490371 23490278 ID=cds.MS.gene63743.t1;Parent=MS.gene63743.t1
Gene Sequence

>MS.gene63743

ATGGAACATCTCTCAATTGGAGGTGAAGAAGATGAGATTGAAATTCCGATTGACCAAAGACAAGCTTCTGCTGATTCTCTTGAGGGTTCCTATGTTCTGGTTGGACGTTTCCTCACAGATCGTCATATTCGATTCCACACGATGAAAGGAAAGATGCCTGAATTCATGCAACCGGTCTGCCAAATGACTATCAAGGAGGTCGATGCCAATCTGTTTCTATTCCAGTTCTTTCATCCTCGTGATATGGAACACGTTCTCAAAAAAGGACCGTGGTCTTTTGATGGGCACACTCTGGTTCTAGGGTTTCTACAACAAGGACTCACACCTAAATCTGTTCCTCTAAATCACATACCCTTTTGGGTGCAAATCCATGAGGTCCCAGTCGGTTGTATGACAATTACTGCACGTAAACAACTTGGCAACTTTATTGGAGAATATATGGAATATGATGAGAAAAATAATTCAAATTTCCTAGCTTCATATATGCGTATTCGAGTACTGATTGATGTCCGTAAACCCTTGGCGAGATTTAAGAAGATTAGGACTAAAGCTGGATCACATGAAGTTCGTTTCAAATATGAGAGACTTGGGTCCTTTTGTTATTACTGTGGTTTGTTAGGCCATACTGATGAATTTTGCAGCAGCCTTTATGCGAATACTGATGATGATGGTACACGTTTATGGAGTTCTGACTTACGTGCATTAACAAAGAGGAATGGAAGTGGAGGTTCTACATCCAAGTGGCTGCGCGAGGATGGTGGTCCAATCAGGGCTGCTGCACCATCACATAAGGCAGGAACAACAACATCCATGAATACTACATCTGTGCCTGTTACGGGTGGGAGCACTCAACAACAGTTAAACGTGCCAACAGTTGTTAATCAAATAGGGGCTCATATATCCATGGCTGAAGCATTCTCAAACCCTGCAACTTTATTTCCTCAACCACCGGCTAAATTAACTTCGGCAGCTCATAAACCTGTTGTGAATGAATCTGAATTAATTGAGGAAGAATTAGATGGCTCCTCAAAGAAAAGAACAAGGTGCAATAATTCCAGTAGCACATCAGCTGACACTACAAATCTTAATGGCTCACCTGAGACGAAAGCTATGGACGGAGTGCAACTTTCTGTTCTACAACAAAGCACAATAGATAAGCAGCATTTTTTATGGACAGAGTCTGGTTCCCAGGCCTGCCGGACGAAATGAGTATTTTATGTTGGAACTGCCGAGGCCTGGGACATCCTAAGGCAGTTCCTAGTCTTAAAGACCTTATTCGTGTCTATAAACCGGATATTTTTTTCCTTATTGAAACTTTGTCGCATTCTAATAAAATAAGAGATGTTTGTTATATGCTTGGCTATGATTCATATTTTACAGTTGATCGAGAAGGCTGTAGTGGTGGCCTTGCTATTATTTGGAAATCATCTTTTCAATGTAGTATTACTAATTATTCATCTAATTTTATTAATCTGCAAGTAAATGATCCAGTTAGAGGTAATTGGCGCCTAACTTCTTTCTGGGGTTACTCTAACACAGCTCGGCGTAGACAATCATGGAATCTTCTTCGTGACCTTGCCTCTATGTCTAACGACCCTTGGTGTATCATAGGTGATTTTAATGACCTCCTTACAAGCGATGACAAACGAGGAGGATCCGAGAGACCTCACTGGCTCTATAATGGATTCCGATCAGCTGTCATTGATTGTAACTTGCATGACCTCCCATTAGAAGGATATCAATTCACATGGTTCAAAAGCCTTGGGACCCCACGTGTTGTTGAAGAAAGACTCGATCGCGCTTTCTATACATCCTCATGGCTCACTCTTTTCCCGAACGCACAACTTATCAATCTTGTTGCAGCCACTTCCGATCATTCACCTATTCTACTACATCTGGAACCCGAAAATTATATTCACTCTAAGCGTAGCTTTCGCTTTGAAAATTCATGGCTCCTTGATCCAAGTTTCTCCGACATGGTTGATGAGAATTGGCATTACTATCCATCTAATAACATCATGCAAAAACTCAAGTATTGTGTTGACGACATGGAAGCTTAG
Protein sequence

>MS.gene63743.t1

MEHLSIGGEEDEIEIPIDQRQASADSLEGSYVLVGRFLTDRHIRFHTMKGKMPEFMQPVCQMTIKEVDANLFLFQFFHPRDMEHVLKKGPWSFDGHTLVLGSLYANTDDDGTRLWSSDLRALTKRNGSGGSTSKWLREDGGPIRAAAPSHKAGTTTSMNTTSVPVTGGSTQQQLNVPTVVNQIGAHISMAEAFSNPATLFPQPPAKLTSAAHKPVVNESELIEEELDGSSKKRTSFSDMVDENWHYYPSNNIMQKLKYCVDDMEA