AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007022


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007022.t1 MTR_0010s0370 34.694 147 95 1 35 180 34 180 3.29e-27 106
MS.gene007022.t1 MTR_0010s0350 34.694 147 95 1 35 180 34 180 3.29e-27 106
MS.gene007022.t1 MTR_6g045237 26.163 172 126 1 44 214 93 264 8.54e-14 72.8
MS.gene007022.t1 MTR_6g033215 23.699 173 126 3 29 197 123 293 8.93e-14 71.6
MS.gene007022.t1 MTR_0008s0180 35.135 111 37 2 74 184 19 94 2.00e-12 64.7
MS.gene007022.t1 MTR_0008s0210 35.135 111 37 2 74 184 38 113 2.43e-12 64.7
MS.gene007022.t1 MTR_2g061500 25.000 112 84 0 17 128 18 129 1.88e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007022.t1 AT3G31430 26.744 172 118 4 46 214 145 311 3.27e-17 82.0
MS.gene007022.t1 AT2G01050 24.631 203 147 4 13 214 59 256 4.12e-17 83.2

Find 0 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGACTTGAAAGATATTAAT+CGG - 51720:23688-23707 MS.gene007022:CDS 20.0%
! AAAACAAGCAAGAAAAACAA+AGG - 51720:24254-24273 MS.gene007022:CDS 25.0%
! AAACAAGCAAGAAAAACAAA+GGG - 51720:24255-24274 MS.gene007022:CDS 25.0%
! TTGAAAGATATTAATCGGAT+GGG - 51720:23693-23712 MS.gene007022:CDS 25.0%
ACTTCCAAGGAAAAGAAATT+TGG - 51720:23781-23800 MS.gene007022:CDS 30.0%
CTTCCAAGGAAAAGAAATTT+GGG - 51720:23782-23801 MS.gene007022:CDS 30.0%
CTTGAAAGATATTAATCGGA+TGG - 51720:23692-23711 MS.gene007022:CDS 30.0%
!! TGACCTTTGCTATTATTAGA+GGG + 51720:24038-24057 None:intergenic 30.0%
AGAAAAACAAAGGGAGACAA+TGG - 51720:24264-24283 MS.gene007022:CDS 35.0%
AGGTAGTTTGAAAAATGGTG+AGG - 51720:24124-24143 MS.gene007022:CDS 35.0%
GAAAAACAAAGGGAGACAAT+GGG - 51720:24265-24284 MS.gene007022:CDS 35.0%
GACAACAATTGGAAGTGATT+TGG - 51720:24328-24347 MS.gene007022:CDS 35.0%
GGTAGTTTGAAAAATGGTGA+GGG - 51720:24125-24144 MS.gene007022:CDS 35.0%
TCCTTCAGATCTGAGTTTAA+GGG + 51720:23625-23644 None:intergenic 35.0%
TGGTTTCATACGTCATCTTA+GGG + 51720:23971-23990 None:intergenic 35.0%
TTCCTTCAGATCTGAGTTTA+AGG + 51720:23626-23645 None:intergenic 35.0%
TTGAAGAAGCCACTCAAAAA+GGG - 51720:23747-23766 MS.gene007022:CDS 35.0%
TTGGTTTCATACGTCATCTT+AGG + 51720:23972-23991 None:intergenic 35.0%
TTTCAAACAGACAATTACCC+AGG - 51720:23383-23402 MS.gene007022:CDS 35.0%
TTTGAAGAAGCCACTCAAAA+AGG - 51720:23746-23765 MS.gene007022:CDS 35.0%
! TTGAAAGCACGAATGTTGTA+GGG + 51720:23369-23388 None:intergenic 35.0%
! TTTGAAAGCACGAATGTTGT+AGG + 51720:23370-23389 None:intergenic 35.0%
!! AAGGAACCTTTGAGATTGAA+GGG - 51720:24171-24190 MS.gene007022:CDS 35.0%
!! ACCTTTGCTATTATTAGAGG+GGG + 51720:24036-24055 None:intergenic 35.0%
!! CTGACCTTTGCTATTATTAG+AGG + 51720:24039-24058 None:intergenic 35.0%
!! GACCTTTGCTATTATTAGAG+GGG + 51720:24037-24056 None:intergenic 35.0%
AACTCAGATACCAGGAAAGA+AGG - 51720:24059-24078 MS.gene007022:CDS 40.0%
AAGGAATTGCTAAGAAGCTG+GGG - 51720:23640-23659 MS.gene007022:CDS 40.0%
AGACGAAATCTCAGAAGATG+AGG - 51720:23299-23318 MS.gene007022:CDS 40.0%
AGAGGATGATACAATCACAG+TGG - 51720:23272-23291 MS.gene007022:CDS 40.0%
AGGTTTCTGTCAAAACTCCA+AGG + 51720:23513-23532 None:intergenic 40.0%
ATCATAAACCCTAACCCAGA+AGG + 51720:23598-23617 None:intergenic 40.0%
CAACCAACAGAGACAACAAT+TGG - 51720:24317-24336 MS.gene007022:CDS 40.0%
CAATTGTTGTCTCTGTTGGT+TGG + 51720:24319-24338 None:intergenic 40.0%
CCAAGGAAAAGAAATTTGGG+TGG - 51720:23785-23804 MS.gene007022:CDS 40.0%
CCTTCAGATCTGAGTTTAAG+GGG + 51720:23624-23643 None:intergenic 40.0%
CTTCAAATTCGCAACCAAGA+AGG - 51720:23461-23480 MS.gene007022:CDS 40.0%
CTTCCAATTGTTGTCTCTGT+TGG + 51720:24323-24342 None:intergenic 40.0%
GAAGGAATTGCTAAGAAGCT+GGG - 51720:23639-23658 MS.gene007022:CDS 40.0%
GCTGGAGGTAGTTTGAAAAA+TGG - 51720:24119-24138 MS.gene007022:CDS 40.0%
GTATCATCCTCTTTCAGATC+TGG + 51720:23264-23283 None:intergenic 40.0%
TAAGCAACCAGATCTGAAAG+AGG - 51720:23254-23273 MS.gene007022:CDS 40.0%
TCAGATCTTGTATGTCGACA+GGG + 51720:23425-23444 None:intergenic 40.0%
TCATAAACCCTAACCCAGAA+GGG + 51720:23597-23616 None:intergenic 40.0%
TCCCCCTCTAATAATAGCAA+AGG - 51720:24032-24051 MS.gene007022:CDS 40.0%
TCTTGGTTGCGAATTTGAAG+AGG + 51720:23461-23480 None:intergenic 40.0%
TGAAGAAGCCACTCAAAAAG+GGG - 51720:23748-23767 MS.gene007022:CDS 40.0%
TGAAGGAATTGCTAAGAAGC+TGG - 51720:23638-23657 MS.gene007022:CDS 40.0%
TGACAGAAACCTTGTCATCT+TGG - 51720:23521-23540 MS.gene007022:CDS 40.0%
TGGGCAAATTTCTCAGAATC+CGG - 51720:23712-23731 MS.gene007022:CDS 40.0%
TTCAGATCTTGTATGTCGAC+AGG + 51720:23426-23445 None:intergenic 40.0%
! CCACCCAAATTTCTTTTCCT+TGG + 51720:23788-23807 None:intergenic 40.0%
! TGAAAGCACGAATGTTGTAG+GGG + 51720:23368-23387 None:intergenic 40.0%
!! AGGAACCTTTGAGATTGAAG+GGG - 51720:24172-24191 MS.gene007022:CDS 40.0%
!! ATCTTTGACCCCTTTTTGAG+TGG + 51720:23759-23778 None:intergenic 40.0%
!! CAAGGAACCTTTGAGATTGA+AGG - 51720:24170-24189 MS.gene007022:CDS 40.0%
AAGGTCAGAACTCAGATACC+AGG - 51720:24051-24070 MS.gene007022:CDS 45.0%
ACAATGGGTCCGACAAAAGA+GGG - 51720:24280-24299 MS.gene007022:CDS 45.0%
ACCAAGAGAGAAACAAGCTC+TGG - 51720:23987-24006 MS.gene007022:CDS 45.0%
AGATACCAGGAAAGAAGGAG+GGG - 51720:24064-24083 MS.gene007022:CDS 45.0%
ATACTCGGTCCAAGATGACA+AGG + 51720:23533-23552 None:intergenic 45.0%
CAAATTCGCAACCAAGAAGG+AGG - 51720:23464-23483 MS.gene007022:CDS 45.0%
CAACAACATGAACAAGCTGC+TGG - 51720:24101-24120 MS.gene007022:CDS 45.0%
CAGATACCAGGAAAGAAGGA+GGG - 51720:24063-24082 MS.gene007022:CDS 45.0%
CCAAGAGAGAAACAAGCTCT+GGG - 51720:23988-24007 MS.gene007022:CDS 45.0%
CCCAATTTCTGCTTTGCTTG+CGG - 51720:23828-23847 MS.gene007022:CDS 45.0%
CCCCTTAAACTCAGATCTGA+AGG - 51720:23621-23640 MS.gene007022:CDS 45.0%
CCGCAAGCAAAGCAGAAATT+GGG + 51720:23831-23850 None:intergenic 45.0%
CGCAAGCAAAGCAGAAATTG+GGG + 51720:23830-23849 None:intergenic 45.0%
CTACAGTGAACTTGAGGAGA+AGG - 51720:23908-23927 MS.gene007022:CDS 45.0%
CTGTGAGGAAATTGAAGACC+AGG - 51720:23875-23894 MS.gene007022:CDS 45.0%
CTTCTCCTCAAGTTCACTGT+AGG + 51720:23910-23929 None:intergenic 45.0%
GGATGATACAATCACAGTGG+AGG - 51720:23275-23294 MS.gene007022:CDS 45.0%
GGGCAAATTTCTCAGAATCC+GGG - 51720:23713-23732 MS.gene007022:CDS 45.0%
GGGTCAAAGATCAACTTCCA+AGG - 51720:23768-23787 MS.gene007022:CDS 45.0%
GTATTCTCGCGTACCTTAGT+CGG - 51720:23324-23343 MS.gene007022:CDS 45.0%
TATTCTCGCGTACCTTAGTC+GGG - 51720:23325-23344 MS.gene007022:CDS 45.0%
TCAGATACCAGGAAAGAAGG+AGG - 51720:24062-24081 MS.gene007022:CDS 45.0%
TCATACGTCATCTTAGGGAG+TGG + 51720:23966-23985 None:intergenic 45.0%
TCTTCAAATCAAGAGCGACC+CGG + 51720:23734-23753 None:intergenic 45.0%
TGATGCCTACAGTGAACTTG+AGG - 51720:23902-23921 MS.gene007022:CDS 45.0%
TGGTGTGTAGAAATGGACCT+TGG - 51720:23493-23512 MS.gene007022:CDS 45.0%
TTGTATGTCGACAGGGTTTC+TGG + 51720:23418-23437 None:intergenic 45.0%
!! AACCTTTGAGATTGAAGGGG+TGG - 51720:24175-24194 MS.gene007022:CDS 45.0%
AAAGGTTCCTTGTGCAGCCT+TGG + 51720:24162-24181 None:intergenic 50.0%
AACAGACAATTACCCAGGCC+TGG - 51720:23388-23407 MS.gene007022:CDS 50.0%
AAGAAGCTGGGGAACATCGT+GGG - 51720:23651-23670 MS.gene007022:CDS 50.0%
AAGGTTCCTTGTGCAGCCTT+GGG + 51720:24161-24180 None:intergenic 50.0%
AATGGGTCCGACAAAAGAGG+GGG - 51720:24282-24301 MS.gene007022:CDS 50.0%
ATTCGCAACCAAGAAGGAGG+CGG - 51720:23467-23486 MS.gene007022:CDS 50.0%
ATTTCTGCTTTGCTTGCGGC+AGG - 51720:23832-23851 MS.gene007022:CDS 50.0%
CAACATGAACAAGCTGCTGG+AGG - 51720:24104-24123 MS.gene007022:CDS 50.0%
CAATGGGTCCGACAAAAGAG+GGG - 51720:24281-24300 MS.gene007022:CDS 50.0%
CACTGTAGGCATCATTGTCC+TGG + 51720:23896-23915 None:intergenic 50.0%
CTTAGTCGGGAAGATCTGGA+CGG - 51720:23338-23357 MS.gene007022:CDS 50.0%
GACAATGGGTCCGACAAAAG+AGG - 51720:24279-24298 MS.gene007022:CDS 50.0%
GCCGCAAGCAAAGCAGAAAT+TGG + 51720:23832-23851 None:intergenic 50.0%
GCGGATCTGGTGTGTAGAAA+TGG - 51720:23486-23505 MS.gene007022:CDS 50.0%
GCTGCTCATTGCCTGATACT+CGG + 51720:23548-23567 None:intergenic 50.0%
GTACCTTAGTCGGGAAGATC+TGG - 51720:23334-23353 MS.gene007022:CDS 50.0%
GTCATCTTGGACCGAGTATC+AGG - 51720:23534-23553 MS.gene007022:CDS 50.0%
TAAGAAGCTGGGGAACATCG+TGG - 51720:23650-23669 MS.gene007022:CDS 50.0%
! AGGACCAAGCTTTTGGACCT+TGG - 51720:23928-23947 MS.gene007022:CDS 50.0%
! CCCAGAGCTTGTTTCTCTCT+TGG + 51720:23991-24010 None:intergenic 50.0%
! GAGGAGAAGGACCAAGCTTT+TGG - 51720:23921-23940 MS.gene007022:CDS 50.0%
AACCAAGAAGGAGGCGGATC+TGG - 51720:23473-23492 MS.gene007022:CDS 55.0%
ATCAGGCAATGAGCAGCCGT+CGG - 51720:23551-23570 MS.gene007022:CDS 55.0%
CAGGAAAGAAGGAGGGGAAG+AGG - 51720:24070-24089 MS.gene007022:CDS 55.0%
CGAGACTGCTCCCAACGATT+CGG + 51720:24201-24220 None:intergenic 55.0%
CGCTCTCAACCTCCTCAACA+GGG - 51720:24219-24238 MS.gene007022:CDS 55.0%
CGTCCAGATCTTCCCGACTA+AGG + 51720:23340-23359 None:intergenic 55.0%
CTCTTCCCCTCCTTCTTTCC+TGG + 51720:24072-24091 None:intergenic 55.0%
GAAAGAAGGAGGGGAAGAGG+TGG - 51720:24073-24092 MS.gene007022:CDS 55.0%
GCTCTCAACCTCCTCAACAG+GGG - 51720:24220-24239 MS.gene007022:CDS 55.0%
GGCCACCCCTTCAATCTCAA+AGG + 51720:24180-24199 None:intergenic 55.0%
TCCTCAACAGGGGTGAGCAA+AGG - 51720:24230-24249 MS.gene007022:CDS 55.0%
TCCTTTGCTCACCCCTGTTG+AGG + 51720:24234-24253 None:intergenic 55.0%
TCGCTCTCAACCTCCTCAAC+AGG - 51720:24218-24237 MS.gene007022:CDS 55.0%
TGAGGGAGAAGTAGCACCCA+AGG - 51720:24142-24161 MS.gene007022:CDS 55.0%
TGCTTTGCTTGCGGCAGGAT+TGG - 51720:23837-23856 MS.gene007022:CDS 55.0%
TTTCCTCACAGTCGCGCATC+TGG + 51720:23866-23885 None:intergenic 55.0%
TTTGCTCACCCCTGTTGAGG+AGG + 51720:24231-24250 None:intergenic 55.0%
! GACAGGGTTTCTGGATCTCC+AGG + 51720:23409-23428 None:intergenic 55.0%
! GTCTCTGTTGGTTGGCTCCT+CGG + 51720:24311-24330 None:intergenic 55.0%
! GTTTCTGGATCTCCAGGCCT+GGG + 51720:23403-23422 None:intergenic 55.0%
! TTAGGGAGTGGTGATGCACG+CGG + 51720:23954-23973 None:intergenic 55.0%
ACACAGCGCCCTTCTGGGTT+AGG - 51720:23586-23605 MS.gene007022:CDS 60.0%
ACACCAGATGCGCGACTGTG+AGG - 51720:23860-23879 MS.gene007022:CDS 60.0%
CAATGAGCAGCCGTCGGAGA+TGG - 51720:23557-23576 MS.gene007022:CDS 60.0%
CACAGCGCCCTTCTGGGTTA+GGG - 51720:23587-23606 MS.gene007022:CDS 60.0%
CACCAGATCCGCCTCCTTCT+TGG + 51720:23478-23497 None:intergenic 60.0%
GAAGGGGTGGCCGAATCGTT+GGG - 51720:24188-24207 MS.gene007022:CDS 60.0%
GATGGACACAGCGCCCTTCT+GGG - 51720:23581-23600 MS.gene007022:CDS 60.0%
GCGGCCAAGGTCCAAAAGCT+TGG + 51720:23935-23954 None:intergenic 60.0%
GTAGCACCCAAGGCTGCACA+AGG - 51720:24152-24171 MS.gene007022:CDS 60.0%
GTGTCCATCGCCATCTCCGA+CGG + 51720:23570-23589 None:intergenic 60.0%
TGAAGGGGTGGCCGAATCGT+TGG - 51720:24187-24206 MS.gene007022:CDS 60.0%
! GGTTTCTGGATCTCCAGGCC+TGG + 51720:23404-23423 None:intergenic 60.0%
! TCGGCCTCCCCCTCTTTTGT+CGG + 51720:24292-24311 None:intergenic 60.0%
CGATGGACACAGCGCCCTTC+TGG - 51720:23580-23599 MS.gene007022:CDS 65.0%
GACAAAAGAGGGGGAGGCCG+AGG - 51720:24291-24310 MS.gene007022:CDS 65.0%
GGGTCCGACAAAAGAGGGGG+AGG - 51720:24285-24304 MS.gene007022:CDS 65.0%
! AGTGGTGATGCACGCGGCCA+AGG + 51720:23948-23967 None:intergenic 65.0%
GCAGCCGTCGGAGATGGCGA+TGG - 51720:23563-23582 MS.gene007022:CDS 70.0%


Chromosome Type Strat End Strand Name
51720 gene 23237 24350 23237 ID=MS.gene007022
51720 mRNA 23237 24350 23237 ID=MS.gene007022.t1;Parent=MS.gene007022
51720 exon 23237 24350 23237 ID=MS.gene007022.t1.exon1;Parent=MS.gene007022.t1
51720 CDS 23237 24350 23237 ID=cds.MS.gene007022.t1;Parent=MS.gene007022.t1
Gene Sequence

>MS.gene007022

ATGGCAAAACGTGACGATAAGCAACCAGATCTGAAAGAGGATGATACAATCACAGTGGAGGCAGACGAAATCTCAGAAGATGAGGAAGTATTCTCGCGTACCTTAGTCGGGAAGATCTGGACGGAAAGCCCCTACAACATTCGTGCTTTCAAACAGACAATTACCCAGGCCTGGAGATCCAGAAACCCTGTCGACATACAAGATCTGAACAAGAATTTGTTCCTCTTCAAATTCGCAACCAAGAAGGAGGCGGATCTGGTGTGTAGAAATGGACCTTGGAGTTTTGACAGAAACCTTGTCATCTTGGACCGAGTATCAGGCAATGAGCAGCCGTCGGAGATGGCGATGGACACAGCGCCCTTCTGGGTTAGGGTTTATGATCTACCCCTTAAACTCAGATCTGAAGGAATTGCTAAGAAGCTGGGGAACATCGTGGGTCAGTTTCTTGAGCTAGACTTGAAAGATATTAATCGGATGGGCAAATTTCTCAGAATCCGGGTCGCTCTTGATTTGAAGAAGCCACTCAAAAAGGGGTCAAAGATCAACTTCCAAGGAAAAGAAATTTGGGTGGATTACAAGTATGAGAGACTCCCCAATTTCTGCTTTGCTTGCGGCAGGATTGGACACCAGATGCGCGACTGTGAGGAAATTGAAGACCAGGACAATGATGCCTACAGTGAACTTGAGGAGAAGGACCAAGCTTTTGGACCTTGGCCGCGTGCATCACCACTCCCTAAGATGACGTATGAAACCAAGAGAGAAACAAGCTCTGGGAACTGCAGCAAGAACTTATTTTCCCCCTCTAATAATAGCAAAGGTCAGAACTCAGATACCAGGAAAGAAGGAGGGGAAGAGGTGGATCAACAACAACATGAACAAGCTGCTGGAGGTAGTTTGAAAAATGGTGAGGGAGAAGTAGCACCCAAGGCTGCACAAGGAACCTTTGAGATTGAAGGGGTGGCCGAATCGTTGGGAGCAGTCTCGCTCTCAACCTCCTCAACAGGGGTGAGCAAAGGAAAAACAAGCAAGAAAAACAAAGGGAGACAATGGGTCCGACAAAAGAGGGGGAGGCCGAGGAGCCAACCAACAGAGACAACAATTGGAAGTGATTTGG

Protein sequence

>MS.gene007022.t1

MAKRDDKQPDLKEDDTITVEADEISEDEEVFSRTLVGKIWTESPYNIRAFKQTITQAWRSRNPVDIQDLNKNLFLFKFATKKEADLVCRNGPWSFDRNLVILDRVSGNEQPSEMAMDTAPFWVRVYDLPLKLRSEGIAKKLGNIVGQFLELDLKDINRMGKFLRIRVALDLKKPLKKGSKINFQGKEIWVDYKYERLPNFCFACGRIGHQMRDCEEIEDQDNDAYSELEEKDQAFGPWPRASPLPKMTYETKRETSSGNCSKNLFSPSNNSKGQNSDTRKEGGEEVDQQQHEQAAGGSLKNGEGEVAPKAAQGTFEIEGVAESLGAVSLSTSSTGVSKGKTSKKNKGRQWVRQKRGRPRSQPTETTIGSDL