AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042027

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042027.t1 MTR_3g053920 36.726 226 116 6 1 217 1 208 1.06e-36 135
MS.gene042027.t1 MTR_2g061500 50.000 92 46 0 1 92 1 92 1.74e-24 98.6
MS.gene042027.t1 MTR_8g463110 45.098 102 56 0 332 433 217 318 2.78e-23 103
MS.gene042027.t1 MTR_4g020630 47.059 102 54 0 332 433 153 254 7.17e-23 101
MS.gene042027.t1 MTR_6g034215 45.098 102 56 0 332 433 221 322 2.09e-22 97.8
MS.gene042027.t1 MTR_1g100687 46.667 75 40 0 334 408 157 231 1.60e-16 79.0
MS.gene042027.t1 MTR_7g063500 43.333 60 34 0 374 433 37 96 1.70e-11 62.0
MS.gene042027.t1 MTR_3g044860 36.364 99 37 1 332 430 109 181 3.19e-11 64.3
MS.gene042027.t1 MTR_5g069440 43.590 78 40 2 357 433 33 107 5.44e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 104 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCTGCCACTCGTGTTTC+AGG 0.155042 6.2:-64366913 None:intergenic
AGAACCAAGATTTGATTATA+TGG 0.304906 6.2:+64368471 MS.gene042027:CDS
GGATGATGGGTCACGTGGTT+GGG 0.310299 6.2:+64367060 MS.gene042027:CDS
AACCTGCTGCTGGCACTTGC+TGG 0.313600 6.2:-64367174 None:intergenic
TGGCTCAGGATGATGCTTAT+TGG 0.318888 6.2:+64368707 MS.gene042027:CDS
GTTCAGCTTCAACCAATATA+TGG 0.332486 6.2:+64367116 MS.gene042027:CDS
GTGCTCACGCCGGTTTGATA+TGG 0.347598 6.2:+64367033 MS.gene042027:CDS
TTCAACCAATATATGGCTTC+AGG 0.358822 6.2:+64367123 MS.gene042027:CDS
AGGTTTCAATGGCTTGCATA+TGG 0.362801 6.2:+64367192 MS.gene042027:CDS
CATGCACAGGTTGCTGGCTC+AGG 0.369810 6.2:+64368693 MS.gene042027:CDS
GATAAGGGAATGAGTCTCTT+AGG 0.371406 6.2:+64367620 MS.gene042027:CDS
CAAGCGCATGCACAGGTTGC+TGG 0.375452 6.2:+64368687 MS.gene042027:CDS
CGGATAGAGTGAGGCCTTCA+AGG 0.390593 6.2:-64366407 None:intergenic
ATTCTAGACCACTTCCTGAT+TGG 0.390648 6.2:+64366477 MS.gene042027:CDS
CTTCACTGGACGCCAGATAT+AGG 0.392435 6.2:-64366553 None:intergenic
TTTACCATATAATCAAATCT+TGG 0.397079 6.2:-64368475 None:intergenic
AGGATCCTGAAGCCATATAT+TGG 0.404648 6.2:-64367128 None:intergenic
TTCAGGATCCTATTCCTTCA+CGG 0.405219 6.2:+64367140 MS.gene042027:CDS
GTCTAATGTAGATGGGGATA+AGG 0.405876 6.2:+64367604 MS.gene042027:CDS
GGTCACGTGGTTGGGGAGAA+TGG 0.408683 6.2:+64367068 MS.gene042027:CDS
ACCTCTGACCTTAACCTTGA+AGG 0.415403 6.2:+64366393 MS.gene042027:CDS
CATCCAGACAGTGATTGAAT+TGG 0.418999 6.2:-64366617 None:intergenic
TATCTGCCACTCGTGTTTCA+GGG 0.420811 6.2:-64366912 None:intergenic
GTGCCAGCAGCAGGTTTCAA+TGG 0.422032 6.2:+64367181 MS.gene042027:CDS
AGGAACGCATGGCCTATATC+TGG 0.424817 6.2:+64366541 MS.gene042027:CDS
TGCTATTTGTGTGGCTGCCT+AGG 0.430802 6.2:+64366995 MS.gene042027:CDS
GAGTGAGGCCTTCAAGGTTA+AGG 0.432136 6.2:-64366401 None:intergenic
TCTAATGTAGATGGGGATAA+GGG 0.438404 6.2:+64367605 MS.gene042027:CDS
GACCAGCAAGTGCCAGCAGC+AGG 0.444851 6.2:+64367172 MS.gene042027:CDS
GTTCCAATTCAATCACTGTC+TGG 0.447913 6.2:+64366614 MS.gene042027:CDS
AGGGTGCAGAGTTGAAGAAT+TGG 0.458768 6.2:+64367522 MS.gene042027:CDS
CAGTGAGCACACGTCCAATC+AGG 0.459668 6.2:-64366491 None:intergenic
TGAATTTGATTCCGAGGCAC+AGG 0.459988 6.2:+64367502 MS.gene042027:CDS
TGAAGGATTGACTCTCAATC+TGG 0.463556 6.2:+64366431 MS.gene042027:CDS
AACTGACTCTGGATTGTCTT+CGG 0.465718 6.2:-64367576 None:intergenic
TGCTGAACCCGTGAAGGAAT+AGG 0.469425 6.2:-64367148 None:intergenic
AGGATGATGGGTCACGTGGT+TGG 0.473500 6.2:+64367059 MS.gene042027:CDS
GATGATGGGTCACGTGGTTG+GGG 0.474373 6.2:+64367061 MS.gene042027:CDS
TCAGGATCCTATTCCTTCAC+GGG 0.475085 6.2:+64367141 MS.gene042027:CDS
AAAGCAAGGACAACTTGGTT+AGG 0.477425 6.2:-64367428 None:intergenic
TCACTGTCTGGATGCTATGA+AGG 0.478442 6.2:+64366626 MS.gene042027:CDS
TCACTTCTTCCACAAATGAC+TGG 0.478528 6.2:+64367389 MS.gene042027:CDS
TAAGCTGAAAATTGAAATTG+AGG 0.481585 6.2:+64368591 MS.gene042027:CDS
CAAGCAAAGCAAGGACAACT+TGG 0.483223 6.2:-64367433 None:intergenic
TTAGACTCTAAAACTGACTC+TGG 0.498368 6.2:-64367587 None:intergenic
ACATTATTTCCAGTCATTTG+TGG 0.500782 6.2:-64367398 None:intergenic
ACGTGTGCTCACTGACAAAC+AGG 0.501707 6.2:+64366500 MS.gene042027:CDS
ACATCTTATCAGTATGTCCT+AGG 0.504777 6.2:-64367012 None:intergenic
GGTTTCAATGGCTTGCATAT+GGG 0.505285 6.2:+64367193 MS.gene042027:CDS
ACTTCGAACTCGACATCGTC+CGG 0.505768 6.2:+64367217 MS.gene042027:CDS
TGAAACACGAGTGGCAGATA+CGG 0.508378 6.2:+64366915 MS.gene042027:CDS
ATTATATGGTAAATGAGTGT+TGG 0.520623 6.2:+64368485 MS.gene042027:CDS
GAAGGTTATTAAAGAAGCTA+AGG 0.521887 6.2:+64366644 MS.gene042027:CDS
CAACTCTGCACCCTGTGCCT+CGG 0.522009 6.2:-64367513 None:intergenic
CATCCTCCTCCATATCAAAC+CGG 0.529616 6.2:-64367042 None:intergenic
TTTGATATGGAGGAGGATGA+TGG 0.529877 6.2:+64367046 MS.gene042027:CDS
GCTATGGTAACCTTCTTCAC+TGG 0.537104 6.2:-64366567 None:intergenic
ATTTAGCAAAGCGCAACTTC+CGG 0.539711 6.2:+64368428 MS.gene042027:intron
CTCACGCCGGTTTGATATGG+AGG 0.542061 6.2:+64367036 MS.gene042027:CDS
GGCCTCACTCTATCCGATGA+AGG 0.546811 6.2:+64366414 MS.gene042027:CDS
GAAGAAGGTTACCATAGCAC+TGG 0.546945 6.2:+64366572 MS.gene042027:CDS
TATCTGGCGTCCAGTGAAGA+AGG 0.557336 6.2:+64366557 MS.gene042027:CDS
AGCTGCTGAATTTGATTCCG+AGG 0.557856 6.2:+64367496 MS.gene042027:CDS
ACAACGTTGTGCTGAGGAGA+TGG 0.559098 6.2:+64368543 MS.gene042027:CDS
GCGATCGGTAATAAGATGCA+TGG 0.563603 6.2:+64367093 MS.gene042027:CDS
GCTGTGTCAGATGAGAATAC+TGG 0.565666 6.2:+64367455 MS.gene042027:CDS
GCCTTCAAGGTTAAGGTCAG+AGG 0.568343 6.2:-64366394 None:intergenic
AAGCCATTGAAACCTGCTGC+TGG 0.570132 6.2:-64367184 None:intergenic
GAGGATATCATCTTCTGAAG+TGG 0.570466 6.2:-64368512 None:intergenic
GAAACACGAGTGGCAGATAC+GGG 0.573485 6.2:+64366916 MS.gene042027:CDS
TGGCAGATACGGGTAAATGA+TGG 0.577259 6.2:+64366926 MS.gene042027:CDS
ACTTCCGGTTTGAAAATGCA+TGG 0.577640 6.2:+64368443 MS.gene042027:CDS
CTTGCACGCAGTAGTCGCAC+TGG 0.583266 6.2:+64368634 MS.gene042027:CDS
GTATGTGTCATCCAGTGCTA+TGG 0.585062 6.2:-64366583 None:intergenic
ATTGAGAGTCAATCCTTCAT+CGG 0.588374 6.2:-64366427 None:intergenic
TTGATATGGAGGAGGATGAT+GGG 0.589044 6.2:+64367047 MS.gene042027:CDS
GATTGAATTGGAACAAGAAG+CGG 0.594860 6.2:-64366605 None:intergenic
CTGATATTGCCTCTCCAAGT+GGG 0.597883 6.2:+64367354 MS.gene042027:CDS
ATCTGCCACTCGTGTTTCAG+GGG 0.598209 6.2:-64366911 None:intergenic
TGGTCTTGCTGAACCCGTGA+AGG 0.599405 6.2:-64367154 None:intergenic
CGATCGGTAATAAGATGCAT+GGG 0.601198 6.2:+64367094 MS.gene042027:CDS
ACGCCGGTTTGATATGGAGG+AGG 0.606005 6.2:+64367039 MS.gene042027:CDS
ATTAAAGAAGCTAAGGATCG+TGG 0.609851 6.2:+64366651 MS.gene042027:CDS
GCACACGTCCAATCAGGAAG+TGG 0.609977 6.2:-64366485 None:intergenic
TTGGGGAGAATGGCTGCGAT+CGG 0.610606 6.2:+64367078 MS.gene042027:CDS
GAATTTGATTCCGAGGCACA+GGG 0.612643 6.2:+64367503 MS.gene042027:CDS
TCAGCACAACGTTGTTGACG+AGG 0.617525 6.2:-64368535 None:intergenic
TTTAGAGTCTAATGTAGATG+GGG 0.622394 6.2:+64367598 MS.gene042027:CDS
TCGTCAACAACGTTGTGCTG+AGG 0.637782 6.2:+64368537 MS.gene042027:CDS
TGTTACACAAGCGCATGCAC+AGG 0.641252 6.2:+64368680 MS.gene042027:CDS
ACTGATATTGCCTCTCCAAG+TGG 0.642808 6.2:+64367353 MS.gene042027:CDS
CACAACGTTGTTGACGAGGG+AGG 0.648230 6.2:-64368531 None:intergenic
CTGACACAGCAAGCAAAGCA+AGG 0.649849 6.2:-64367442 None:intergenic
ATCCTTCATCGGATAGAGTG+AGG 0.652038 6.2:-64366416 None:intergenic
CTGATAAGATGTGCTCACGC+CGG 0.658592 6.2:+64367023 MS.gene042027:CDS
GTTAAGGTCAGAGGTAGACA+TGG 0.664777 6.2:-64366385 None:intergenic
CAGCACAACGTTGTTGACGA+GGG 0.666833 6.2:-64368534 None:intergenic
GAGGAGGATGATGGGTCACG+TGG 0.677189 6.2:+64367055 MS.gene042027:CDS
GCTTTATGATAACTACAACA+TGG 0.687185 6.2:+64366673 MS.gene042027:CDS
GTGGTTGAACATATTGCACC+CGG 0.690564 6.2:+64366695 MS.gene042027:CDS
CTGCACCCCTGAAACACGAG+TGG 0.698069 6.2:+64366906 MS.gene042027:CDS
TTATGATAACTACAACATGG+TGG 0.709273 6.2:+64366676 MS.gene042027:CDS
GTGCTGAGGAGATGGTAGTG+TGG 0.745343 6.2:+64368551 MS.gene042027:CDS
GTGTCAGATGAGAATACTGG+TGG 0.751380 6.2:+64367458 MS.gene042027:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACCTATATTTTATTATTA+TGG - chr6.2:64367866-64367885 None:intergenic 10.0%
!! TTATTCAACTAATCATATTA+CGG + chr6.2:64367992-64368011 MS.gene042027:intron 15.0%
!!! TCTTTTTCTGTTAATAATAT+TGG + chr6.2:64367912-64367931 MS.gene042027:intron 15.0%
!! GGTCCATAATAATAAAATAT+AGG + chr6.2:64367860-64367879 MS.gene042027:intron 20.0%
!! TAATGAATTTAAACAATCCT+TGG + chr6.2:64367671-64367690 MS.gene042027:intron 20.0%
!! TGATATCATTAATCTCATTA+TGG - chr6.2:64367276-64367295 None:intergenic 20.0%
!! TTAGTAAGCTCAAAAAAATT+TGG + chr6.2:64367320-64367339 MS.gene042027:intron 20.0%
!! TTTACCATATAATCAAATCT+TGG - chr6.2:64368478-64368497 None:intergenic 20.0%
!!! TTTAATGATATTTTGGATGA+CGG + chr6.2:64368127-64368146 MS.gene042027:intron 20.0%
! AAACCATTAATCAACCAATT+AGG - chr6.2:64368178-64368197 None:intergenic 25.0%
! AGAACCAAGATTTGATTATA+TGG + chr6.2:64368471-64368490 MS.gene042027:CDS 25.0%
! ATTATATGGTAAATGAGTGT+TGG + chr6.2:64368485-64368504 MS.gene042027:CDS 25.0%
! CATTTATCTTCTCAATTTGT+AGG + chr6.2:64368082-64368101 MS.gene042027:intron 25.0%
! GTTAATAATATTGGACATAG+TGG + chr6.2:64367921-64367940 MS.gene042027:intron 25.0%
! TAAGCTGAAAATTGAAATTG+AGG + chr6.2:64368591-64368610 MS.gene042027:CDS 25.0%
!! CAATTTTCAGCTTATTACAA+TGG - chr6.2:64368585-64368604 None:intergenic 25.0%
!!! TGGGGATTTTAATGATATTT+TGG + chr6.2:64368120-64368139 MS.gene042027:intron 25.0%
!!! TTAATGATATTTTGGATGAC+GGG + chr6.2:64368128-64368147 MS.gene042027:intron 25.0%
!!! TTTTAGAGTCTAATGTAGAT+GGG + chr6.2:64367597-64367616 MS.gene042027:CDS 25.0%
AAGATGCAAATCATGGTAAT+TGG + chr6.2:64368024-64368043 MS.gene042027:intron 30.0%
AATAGCAGGACTTAAAGATT+AGG + chr6.2:64367711-64367730 MS.gene042027:intron 30.0%
ACATTATTTCCAGTCATTTG+TGG - chr6.2:64367401-64367420 None:intergenic 30.0%
ACTTGTACTATATGGACATT+TGG + chr6.2:64366738-64366757 MS.gene042027:intron 30.0%
AGAAAATGAGTCTGTTAAGT+TGG + chr6.2:64367742-64367761 MS.gene042027:intron 30.0%
CAGTTCTTATATAAGACTGA+AGG + chr6.2:64366868-64366887 MS.gene042027:intron 30.0%
CTTGTACTATATGGACATTT+GGG + chr6.2:64366739-64366758 MS.gene042027:intron 30.0%
GAAGGTTATTAAAGAAGCTA+AGG + chr6.2:64366644-64366663 MS.gene042027:CDS 30.0%
GCTTTATGATAACTACAACA+TGG + chr6.2:64366673-64366692 MS.gene042027:CDS 30.0%
TTATGATAACTACAACATGG+TGG + chr6.2:64366676-64366695 MS.gene042027:CDS 30.0%
! TTTAGAGTCTAATGTAGATG+GGG + chr6.2:64367598-64367617 MS.gene042027:CDS 30.0%
!! AGACAAGTCGTTATTGATTT+AGG + chr6.2:64368199-64368218 MS.gene042027:intron 30.0%
!! TAATGATATTTTGGATGACG+GGG + chr6.2:64368129-64368148 MS.gene042027:intron 30.0%
!! TGTTTTAAGTGTGAGAAGTT+GGG + chr6.2:64366965-64366984 MS.gene042027:CDS 30.0%
!!! CAATTATTTTAAGGAACGCA+TGG + chr6.2:64366530-64366549 MS.gene042027:CDS 30.0%
!!! GTTTTAGAGTCTAATGTAGA+TGG + chr6.2:64367596-64367615 MS.gene042027:CDS 30.0%
!!! TTGTTTTAAGTGTGAGAAGT+TGG + chr6.2:64366964-64366983 MS.gene042027:CDS 30.0%
AAATTTAGTAGCTCCAGCTT+CGG + chr6.2:64368360-64368379 MS.gene042027:intron 35.0%
AACGAAATCGGTAAGTATCT+TGG + chr6.2:64366809-64366828 MS.gene042027:intron 35.0%
AAGCTCTTAAACCAAGTGTA+AGG - chr6.2:64368253-64368272 None:intergenic 35.0%
ACATCTTATCAGTATGTCCT+AGG - chr6.2:64367015-64367034 None:intergenic 35.0%
ATGCAAATCATGGTAATTGG+AGG + chr6.2:64368027-64368046 MS.gene042027:intron 35.0%
ATGTGCATCAACTACCATTT+GGG + chr6.2:64366765-64366784 MS.gene042027:intron 35.0%
ATTAAAGAAGCTAAGGATCG+TGG + chr6.2:64366651-64366670 MS.gene042027:CDS 35.0%
ATTGAGAGTCAATCCTTCAT+CGG - chr6.2:64366430-64366449 None:intergenic 35.0%
CGTCCTAATTGGTTGATTAA+TGG + chr6.2:64368172-64368191 MS.gene042027:intron 35.0%
CTAGGTTAATTCATCCCAAA+TGG - chr6.2:64366782-64366801 None:intergenic 35.0%
GAGAAACAAGATGCAAATCA+TGG + chr6.2:64368017-64368036 MS.gene042027:intron 35.0%
GATTGAATTGGAACAAGAAG+CGG - chr6.2:64366608-64366627 None:intergenic 35.0%
GCTGAAAATGATGTAATAGC+AGG + chr6.2:64367697-64367716 MS.gene042027:intron 35.0%
GTTCAGCTTCAACCAATATA+TGG + chr6.2:64367116-64367135 MS.gene042027:CDS 35.0%
TACACTTGGTTTAAGAGCTT+AGG + chr6.2:64368253-64368272 MS.gene042027:intron 35.0%
TAGCGTGAACTTGTACTATA+TGG + chr6.2:64366730-64366749 MS.gene042027:intron 35.0%
TCTAATGTAGATGGGGATAA+GGG + chr6.2:64367605-64367624 MS.gene042027:CDS 35.0%
TTCAACCAATATATGGCTTC+AGG + chr6.2:64367123-64367142 MS.gene042027:CDS 35.0%
! ACATCATTTTCAGCATACCA+AGG - chr6.2:64367691-64367710 None:intergenic 35.0%
! CTTAACAGACTCATTTTCTG+TGG - chr6.2:64367741-64367760 None:intergenic 35.0%
! GTTTCCATGCATTTTCAAAC+CGG - chr6.2:64368450-64368469 None:intergenic 35.0%
! TAGGACTGTGGTGTATTTTT+GGG + chr6.2:64368101-64368120 MS.gene042027:intron 35.0%
!! CGTTATTGATTTAGGACTCT+CGG + chr6.2:64368207-64368226 MS.gene042027:intron 35.0%
!! GGCTTGGTTCTAATTATTTC+TGG + chr6.2:64368324-64368343 MS.gene042027:intron 35.0%
!! GGTGCGCTTCAATTATTTTA+AGG + chr6.2:64366521-64366540 MS.gene042027:CDS 35.0%
!! GTTTTAAGTGTGAGAAGTTG+GGG + chr6.2:64366966-64366985 MS.gene042027:CDS 35.0%
!! TTAGACTCTAAAACTGACTC+TGG - chr6.2:64367590-64367609 None:intergenic 35.0%
AAAGCAAGGACAACTTGGTT+AGG - chr6.2:64367431-64367450 None:intergenic 40.0%
AAGTTGCGCTTTGCTAAATG+AGG - chr6.2:64368427-64368446 None:intergenic 40.0%
AATTCATTACAACATCCCCC+CGG - chr6.2:64367660-64367679 None:intergenic 40.0%
AGACTGTGCTTTGGCAAATT+AGG + chr6.2:64368303-64368322 MS.gene042027:intron 40.0%
AGGATCCTGAAGCCATATAT+TGG - chr6.2:64367131-64367150 None:intergenic 40.0%
AGTTGCGCTTTGCTAAATGA+GGG - chr6.2:64368426-64368445 None:intergenic 40.0%
ATGTTGCAAAAAATCGCACG+CGG - chr6.2:64368065-64368084 None:intergenic 40.0%
ATTCTAGACCACTTCCTGAT+TGG + chr6.2:64366477-64366496 MS.gene042027:CDS 40.0%
ATTTAGCAAAGCGCAACTTC+CGG + chr6.2:64368428-64368447 MS.gene042027:intron 40.0%
CATCCAGACAGTGATTGAAT+TGG - chr6.2:64366620-64366639 None:intergenic 40.0%
CATGGTAATTGGAGGCTTAT+AGG + chr6.2:64368035-64368054 MS.gene042027:intron 40.0%
CATGTGCATCAACTACCATT+TGG + chr6.2:64366764-64366783 MS.gene042027:intron 40.0%
CGATCGGTAATAAGATGCAT+GGG + chr6.2:64367094-64367113 MS.gene042027:CDS 40.0%
CTTCTCAATTTGTAGGACTG+TGG + chr6.2:64368089-64368108 MS.gene042027:intron 40.0%
GATAAGGGAATGAGTCTCTT+AGG + chr6.2:64367620-64367639 MS.gene042027:CDS 40.0%
GGAAAGATTAGACTGTGCTT+TGG + chr6.2:64368294-64368313 MS.gene042027:intron 40.0%
GGAGTACAGTTCAACAGAAT+AGG - chr6.2:64368394-64368413 None:intergenic 40.0%
GGGATGAATTAACCTAGAGT+GGG + chr6.2:64366785-64366804 MS.gene042027:intron 40.0%
GTCTAATGTAGATGGGGATA+AGG + chr6.2:64367604-64367623 MS.gene042027:CDS 40.0%
GTTCCAATTCAATCACTGTC+TGG + chr6.2:64366614-64366633 MS.gene042027:CDS 40.0%
TAATCTTTCCTCTACCGCAT+GGG - chr6.2:64368284-64368303 None:intergenic 40.0%
TATGGACCAAAGTCTCACTT+AGG - chr6.2:64367848-64367867 None:intergenic 40.0%
TCACTTAGGAAGAGGAATTC+CGG - chr6.2:64367834-64367853 None:intergenic 40.0%
TCACTTCTTCCACAAATGAC+TGG + chr6.2:64367389-64367408 MS.gene042027:CDS 40.0%
TCCCTCTAGATAAGTCTTAC+CGG - chr6.2:64367239-64367258 None:intergenic 40.0%
TCCGGTAAGACTTATCTAGA+GGG + chr6.2:64367235-64367254 MS.gene042027:intron 40.0%
TGGGATGAATTAACCTAGAG+TGG + chr6.2:64366784-64366803 MS.gene042027:intron 40.0%
TTCAGGATCCTATTCCTTCA+CGG + chr6.2:64367140-64367159 MS.gene042027:CDS 40.0%
TTGAGGGATATCCTTACACT+TGG + chr6.2:64368239-64368258 MS.gene042027:intron 40.0%
TTGATATGGAGGAGGATGAT+GGG + chr6.2:64367047-64367066 MS.gene042027:CDS 40.0%
TTTGATATGGAGGAGGATGA+TGG + chr6.2:64367046-64367065 MS.gene042027:CDS 40.0%
! AACTGACTCTGGATTGTCTT+CGG - chr6.2:64367579-64367598 None:intergenic 40.0%
! ACTCTCGGATGTTCATTTTG+AGG + chr6.2:64368222-64368241 MS.gene042027:intron 40.0%
! ACTTCCGGTTTGAAAATGCA+TGG + chr6.2:64368443-64368462 MS.gene042027:CDS 40.0%
! AGGTTTCAATGGCTTGCATA+TGG + chr6.2:64367192-64367211 MS.gene042027:CDS 40.0%
! AGTTCCTGATCTGAAGTATC+TGG + chr6.2:64367794-64367813 MS.gene042027:intron 40.0%
! CAAATGGTAGTTGATGCACA+TGG - chr6.2:64366766-64366785 None:intergenic 40.0%
! CTCTCGGATGTTCATTTTGA+GGG + chr6.2:64368223-64368242 MS.gene042027:intron 40.0%
! GAGGATATCATCTTCTGAAG+TGG - chr6.2:64368515-64368534 None:intergenic 40.0%
! GGTTTCAATGGCTTGCATAT+GGG + chr6.2:64367193-64367212 MS.gene042027:CDS 40.0%
! GTAGGACTGTGGTGTATTTT+TGG + chr6.2:64368100-64368119 MS.gene042027:intron 40.0%
! GTGGTAGTCTTGCTTTATTC+TGG + chr6.2:64367940-64367959 MS.gene042027:intron 40.0%
! TAGGTTTTGCGTTACGTTCT+TGG + chr6.2:64367879-64367898 MS.gene042027:intron 40.0%
! TGAAGGATTGACTCTCAATC+TGG + chr6.2:64366431-64366450 MS.gene042027:CDS 40.0%
!! AGGACTGTGGTGTATTTTTG+GGG + chr6.2:64368102-64368121 MS.gene042027:intron 40.0%
!! TTAGGTGCTGATGTTAGTAC+CGG + chr6.2:64367638-64367657 MS.gene042027:intron 40.0%
!!! GGGACTTTTTGCTATTTGTG+TGG + chr6.2:64366986-64367005 MS.gene042027:CDS 40.0%
AATCTTTCCTCTACCGCATG+GGG - chr6.2:64368283-64368302 None:intergenic 45.0%
ACCTCTGACCTTAACCTTGA+AGG + chr6.2:64366393-64366412 MS.gene042027:CDS 45.0%
ACTGATATTGCCTCTCCAAG+TGG + chr6.2:64367353-64367372 MS.gene042027:CDS 45.0%
AGCTGCTGAATTTGATTCCG+AGG + chr6.2:64367496-64367515 MS.gene042027:CDS 45.0%
AGGGTGCAGAGTTGAAGAAT+TGG + chr6.2:64367522-64367541 MS.gene042027:CDS 45.0%
AGTACAAGTTCACGCTAGCT+GGG - chr6.2:64366727-64366746 None:intergenic 45.0%
AGTTGGAACTGTCGAGGTTT+AGG + chr6.2:64367759-64367778 MS.gene042027:intron 45.0%
ATAGGATAGTGATCCGAAGC+TGG - chr6.2:64368376-64368395 None:intergenic 45.0%
ATCCTTCATCGGATAGAGTG+AGG - chr6.2:64366419-64366438 None:intergenic 45.0%
CAAGCAAAGCAAGGACAACT+TGG - chr6.2:64367436-64367455 None:intergenic 45.0%
CATCCTCCTCCATATCAAAC+CGG - chr6.2:64367045-64367064 None:intergenic 45.0%
CCAAAGTCTCACTTAGGAAG+AGG - chr6.2:64367842-64367861 None:intergenic 45.0%
CCTAGAGTGGGAAACGAAAT+CGG + chr6.2:64366797-64366816 MS.gene042027:intron 45.0%
CCTCTTCCTAAGTGAGACTT+TGG + chr6.2:64367839-64367858 MS.gene042027:intron 45.0%
CTAATCTTTCCTCTACCGCA+TGG - chr6.2:64368285-64368304 None:intergenic 45.0%
CTGATATTGCCTCTCCAAGT+GGG + chr6.2:64367354-64367373 MS.gene042027:CDS 45.0%
GAAGAAGGTTACCATAGCAC+TGG + chr6.2:64366572-64366591 MS.gene042027:CDS 45.0%
GAATTTGATTCCGAGGCACA+GGG + chr6.2:64367503-64367522 MS.gene042027:CDS 45.0%
GCGATCGGTAATAAGATGCA+TGG + chr6.2:64367093-64367112 MS.gene042027:CDS 45.0%
GCTATGGTAACCTTCTTCAC+TGG - chr6.2:64366570-64366589 None:intergenic 45.0%
GCTGTGTCAGATGAGAATAC+TGG + chr6.2:64367455-64367474 MS.gene042027:CDS 45.0%
GGAATTCCGGATTGTAGTGT+CGG - chr6.2:64367821-64367840 None:intergenic 45.0%
GTATGTGTCATCCAGTGCTA+TGG - chr6.2:64366586-64366605 None:intergenic 45.0%
GTCCGGTAAGACTTATCTAG+AGG + chr6.2:64367234-64367253 MS.gene042027:intron 45.0%
GTGCTTTGGCAAATTAGGCT+TGG + chr6.2:64368308-64368327 MS.gene042027:intron 45.0%
GTGGTTGAACATATTGCACC+CGG + chr6.2:64366695-64366714 MS.gene042027:CDS 45.0%
GTGTCAGATGAGAATACTGG+TGG + chr6.2:64367458-64367477 MS.gene042027:CDS 45.0%
GTTGAGGGAAACGTCCTAAT+TGG + chr6.2:64368161-64368180 MS.gene042027:intron 45.0%
TACTAAGCTAGACAGCCATG+AGG - chr6.2:64367306-64367325 None:intergenic 45.0%
TAGTACAAGTTCACGCTAGC+TGG - chr6.2:64366728-64366747 None:intergenic 45.0%
TATCTGCCACTCGTGTTTCA+GGG - chr6.2:64366915-64366934 None:intergenic 45.0%
TCACTGTCTGGATGCTATGA+AGG + chr6.2:64366626-64366645 MS.gene042027:CDS 45.0%
TCAGGATCCTATTCCTTCAC+GGG + chr6.2:64367141-64367160 MS.gene042027:CDS 45.0%
TCTGTTGAACTGTACTCCTG+TGG + chr6.2:64368396-64368415 MS.gene042027:intron 45.0%
TGAAACACGAGTGGCAGATA+CGG + chr6.2:64366915-64366934 MS.gene042027:CDS 45.0%
TGAATTTGATTCCGAGGCAC+AGG + chr6.2:64367502-64367521 MS.gene042027:CDS 45.0%
TGATATCAAGTCTCGCCTCA+TGG + chr6.2:64367288-64367307 MS.gene042027:intron 45.0%
TGGCAGATACGGGTAAATGA+TGG + chr6.2:64366926-64366945 MS.gene042027:CDS 45.0%
TGGCTCAGGATGATGCTTAT+TGG + chr6.2:64368707-64368726 MS.gene042027:CDS 45.0%
! GATGAAGCTTTTGTCCCACT+TGG - chr6.2:64367371-64367390 None:intergenic 45.0%
!! CTGTTAAGTTGGAACTGTCG+AGG + chr6.2:64367753-64367772 MS.gene042027:intron 45.0%
!! TAGGTGCTGATGTTAGTACC+GGG + chr6.2:64367639-64367658 MS.gene042027:intron 45.0%
AAGCCATTGAAACCTGCTGC+TGG - chr6.2:64367187-64367206 None:intergenic 50.0%
ACAACGTTGTGCTGAGGAGA+TGG + chr6.2:64368543-64368562 MS.gene042027:CDS 50.0%
ACGTGTGCTCACTGACAAAC+AGG + chr6.2:64366500-64366519 MS.gene042027:CDS 50.0%
ACTTCGAACTCGACATCGTC+CGG + chr6.2:64367217-64367236 MS.gene042027:CDS 50.0%
AGGAACGCATGGCCTATATC+TGG + chr6.2:64366541-64366560 MS.gene042027:CDS 50.0%
ATCTGCCACTCGTGTTTCAG+GGG - chr6.2:64366914-64366933 None:intergenic 50.0%
CAGCACAACGTTGTTGACGA+GGG - chr6.2:64368537-64368556 None:intergenic 50.0%
CCGATTTCGTTTCCCACTCT+AGG - chr6.2:64366800-64366819 None:intergenic 50.0%
CGGACCAGATACTTCAGATC+AGG - chr6.2:64367801-64367820 None:intergenic 50.0%
CTGACACAGCAAGCAAAGCA+AGG - chr6.2:64367445-64367464 None:intergenic 50.0%
CTGATAAGATGTGCTCACGC+CGG + chr6.2:64367023-64367042 MS.gene042027:CDS 50.0%
CTTCACTGGACGCCAGATAT+AGG - chr6.2:64366556-64366575 None:intergenic 50.0%
GAAACACGAGTGGCAGATAC+GGG + chr6.2:64366916-64366935 MS.gene042027:CDS 50.0%
GAGTGAGGCCTTCAAGGTTA+AGG - chr6.2:64366404-64366423 None:intergenic 50.0%
GCCTTCAAGGTTAAGGTCAG+AGG - chr6.2:64366397-64366416 None:intergenic 50.0%
GTATCTGCCACTCGTGTTTC+AGG - chr6.2:64366916-64366935 None:intergenic 50.0%
TATCTGGCGTCCAGTGAAGA+AGG + chr6.2:64366557-64366576 MS.gene042027:CDS 50.0%
TCAGATCAGGAACTGCACTC+AGG - chr6.2:64367788-64367807 None:intergenic 50.0%
TCAGCACAACGTTGTTGACG+AGG - chr6.2:64368538-64368557 None:intergenic 50.0%
TCGTCAACAACGTTGTGCTG+AGG + chr6.2:64368537-64368556 MS.gene042027:CDS 50.0%
TCTGGTCCGACACTACAATC+CGG + chr6.2:64367812-64367831 MS.gene042027:intron 50.0%
TGCTGAACCCGTGAAGGAAT+AGG - chr6.2:64367151-64367170 None:intergenic 50.0%
TGTTACACAAGCGCATGCAC+AGG + chr6.2:64368680-64368699 MS.gene042027:CDS 50.0%
! ACGGGGAAAAGCATGGTTGA+GGG + chr6.2:64368146-64368165 MS.gene042027:intron 50.0%
! AGCTTTTGTCCCACTTGGAG+AGG - chr6.2:64367366-64367385 None:intergenic 50.0%
! TGCTATTTGTGTGGCTGCCT+AGG + chr6.2:64366995-64367014 MS.gene042027:CDS 50.0%
!! AGGTGCTGATGTTAGTACCG+GGG + chr6.2:64367640-64367659 MS.gene042027:intron 50.0%
!! TTGGATGACGGGGAAAAGCA+TGG + chr6.2:64368139-64368158 MS.gene042027:intron 50.0%
CACAACGTTGTTGACGAGGG+AGG - chr6.2:64368534-64368553 None:intergenic 55.0%
CAGTGAGCACACGTCCAATC+AGG - chr6.2:64366494-64366513 None:intergenic 55.0%
CGGATAGAGTGAGGCCTTCA+AGG - chr6.2:64366410-64366429 None:intergenic 55.0%
GAGCTTAGGTACACCCCATG+CGG + chr6.2:64368267-64368286 MS.gene042027:intron 55.0%
GCACACGTCCAATCAGGAAG+TGG - chr6.2:64366488-64366507 None:intergenic 55.0%
GGCCTCACTCTATCCGATGA+AGG + chr6.2:64366414-64366433 MS.gene042027:CDS 55.0%
GTGCCAGCAGCAGGTTTCAA+TGG + chr6.2:64367181-64367200 MS.gene042027:CDS 55.0%
TTGGGGAGAATGGCTGCGAT+CGG + chr6.2:64367078-64367097 MS.gene042027:CDS 55.0%
! GACGGGGAAAAGCATGGTTG+AGG + chr6.2:64368145-64368164 MS.gene042027:intron 55.0%
! GTGCTGAGGAGATGGTAGTG+TGG + chr6.2:64368551-64368570 MS.gene042027:CDS 55.0%
! GTGCTGATGTTAGTACCGGG+GGG + chr6.2:64367642-64367661 MS.gene042027:intron 55.0%
! TGGTCTTGCTGAACCCGTGA+AGG - chr6.2:64367157-64367176 None:intergenic 55.0%
!! ACGCCGGTTTGATATGGAGG+AGG + chr6.2:64367039-64367058 MS.gene042027:CDS 55.0%
!! AGGATGATGGGTCACGTGGT+TGG + chr6.2:64367059-64367078 MS.gene042027:CDS 55.0%
!! CTCACGCCGGTTTGATATGG+AGG + chr6.2:64367036-64367055 MS.gene042027:CDS 55.0%
!! GATGATGGGTCACGTGGTTG+GGG + chr6.2:64367061-64367080 MS.gene042027:CDS 55.0%
!! GGATGATGGGTCACGTGGTT+GGG + chr6.2:64367060-64367079 MS.gene042027:CDS 55.0%
!! GGTGCTGATGTTAGTACCGG+GGG + chr6.2:64367641-64367660 MS.gene042027:intron 55.0%
!! GTGCTCACGCCGGTTTGATA+TGG + chr6.2:64367033-64367052 MS.gene042027:CDS 55.0%
AACCTGCTGCTGGCACTTGC+TGG - chr6.2:64367177-64367196 None:intergenic 60.0%
AGGTACACCCCATGCGGTAG+AGG + chr6.2:64368273-64368292 MS.gene042027:intron 60.0%
CAACTCTGCACCCTGTGCCT+CGG - chr6.2:64367516-64367535 None:intergenic 60.0%
CAAGCGCATGCACAGGTTGC+TGG + chr6.2:64368687-64368706 MS.gene042027:CDS 60.0%
CTGCACCCCTGAAACACGAG+TGG + chr6.2:64366906-64366925 MS.gene042027:CDS 60.0%
CTTGCACGCAGTAGTCGCAC+TGG + chr6.2:64368634-64368653 MS.gene042027:CDS 60.0%
GGTCACGTGGTTGGGGAGAA+TGG + chr6.2:64367068-64367087 MS.gene042027:CDS 60.0%
! GCTAAATGAGGGCGCACCAC+AGG - chr6.2:64368415-64368434 None:intergenic 60.0%
!! CATGCACAGGTTGCTGGCTC+AGG + chr6.2:64368693-64368712 MS.gene042027:CDS 60.0%
!! GAGGAGGATGATGGGTCACG+TGG + chr6.2:64367055-64367074 MS.gene042027:CDS 60.0%
ACGCTAGCTGGGACGACACC+GGG - chr6.2:64366716-64366735 None:intergenic 65.0%
CACGCTAGCTGGGACGACAC+CGG - chr6.2:64366717-64366736 None:intergenic 65.0%
GACCAGCAAGTGCCAGCAGC+AGG + chr6.2:64367172-64367191 MS.gene042027:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 64366387 64368738 64366387 ID=MS.gene042027
chr6.2 mRNA 64366387 64368738 64366387 ID=MS.gene042027.t1;Parent=MS.gene042027
chr6.2 exon 64366387 64366716 64366387 ID=MS.gene042027.t1.exon1;Parent=MS.gene042027.t1
chr6.2 CDS 64366387 64366716 64366387 ID=cds.MS.gene042027.t1;Parent=MS.gene042027.t1
chr6.2 exon 64366890 64367238 64366890 ID=MS.gene042027.t1.exon2;Parent=MS.gene042027.t1
chr6.2 CDS 64366890 64367238 64366890 ID=cds.MS.gene042027.t1;Parent=MS.gene042027.t1
chr6.2 exon 64367324 64367641 64367324 ID=MS.gene042027.t1.exon3;Parent=MS.gene042027.t1
chr6.2 CDS 64367324 64367641 64367324 ID=cds.MS.gene042027.t1;Parent=MS.gene042027.t1
chr6.2 exon 64368434 64368738 64368434 ID=MS.gene042027.t1.exon4;Parent=MS.gene042027.t1
chr6.2 CDS 64368434 64368738 64368434 ID=cds.MS.gene042027.t1;Parent=MS.gene042027.t1
Gene Sequence

>MS.gene042027

ATGTCTACCTCTGACCTTAACCTTGAAGGCCTCACTCTATCCGATGAAGGATTGACTCTCAATCTGGATGTCGTTACTGCTGAAACACAAATTCTAGACCACTTCCTGATTGGACGTGTGCTCACTGACAAACAGGTGCGCTTCAATTATTTTAAGGAACGCATGGCCTATATCTGGCGTCCAGTGAAGAAGGTTACCATAGCACTGGATGACACATACCGCTTCTTGTTCCAATTCAATCACTGTCTGGATGCTATGAAGGTTATTAAAGAAGCTAAGGATCGTGGCTTTATGATAACTACAACATGGTGGTTGAACATATTGCACCCGGTGTCGTCCCAGCTAGCGTGAACTTGTACTATATGGACATTTGGGTCCATGTGCATCAACTACCATTTGGGATGAATTAACCTAGAGTGGGAAACGAAATCGGTAAGTATCTTGGACACTTAATTGAATTTCATGCTCGCAATACTATTCACAGTTCTTATATAAGACTGAAGGTTCAGATTGATGTTACTGCACCCCTGAAACACGAGTGGCAGATACGGGTAAATGATGGTTCTTATGCAACAATTTGTTTTAAGTGTGAGAAGTTGGGGACTTTTTGCTATTTGTGTGGCTGCCTAGGACATACTGATAAGATGTGCTCACGCCGGTTTGATATGGAGGAGGATGATGGGTCACGTGGTTGGGGAGAATGGCTGCGATCGGTAATAAGATGCATGGGTTCAGCTTCAACCAATATATGGCTTCAGGATCCTATTCCTTCACGGGTTCAGCAAGACCAGCAAGTGCCAGCAGCAGGTTTCAATGGCTTGCATATGGGAACTTCGAACTCGACATCGTCCGGTAAGACTTATCTAGAGGGACGTATTAGTGTTGTCCATAATGAGATTAATGATATCAAGTCTCGCCTCATGGCTGTCTAGCTTAGTAAGCTCAAAAAAATTTGGCATCTCTTTGACTGATATTGCCTCTCCAAGTGGGACAAAAGCTTCATCACTTCTTCCACAAATGACTGGAAATAATGTGCAGCAACCTAACCAAGTTGTCCTTGCTTTGCTTGCTGTGTCAGATGAGAATACTGGTGGTAATAATTATTTTGCAGCTGCTGAATTTGATTCCGAGGCACAGGGTGCAGAGTTGAAGAATTGGAAATGTGCACAAACTGTTGAAGACAAAAGAGCCGAAGACAATCCAGAGTCAGTTTTAGAGTCTAATGTAGATGGGGATAAGGGAATGAGTCTCTTAGGTGCTGATGTTAGTACCGGGGGGATGTTGTAATGAATTTAAACAATCCTTGGTATGCTGAAAATGATGTAATAGCAGGACTTAAAGATTAGGTGTGCCACAGAAAATGAGTCTGTTAAGTTGGAACTGTCGAGGTTTAGGTAGCCTGAGTGCAGTTCCTGATCTGAAGTATCTGGTCCGACACTACAATCCGGAATTCCTCTTCCTAAGTGAGACTTTGGTCCATAATAATAAAATATAGGTTTTGCGTTACGTTCTTGGTTATGATTCTTCTTTTTCTGTTAATAATATTGGACATAGTGGTAGTCTTGCTTTATTCTGGCGTGCATCTTTTAATTGTCAAATTATTAATTATTCAACTAATCATATTACGGTAGAGAAACAAGATGCAAATCATGGTAATTGGAGGCTTATAGGTGTGCCGCGTGCGATTTTTTGCAACATTTATCTTCTCAATTTGTAGGACTGTGGTGTATTTTTGGGGATTTTAATGATATTTTGGATGACGGGGAAAAGCATGGTTGAGGGAAACGTCCTAATTGGTTGATTAATGGTTTCAGACAAGTCGTTATTGATTTAGGACTCTCGGATGTTCATTTTGAGGGATATCCTTACACTTGGTTTAAGAGCTTAGGTACACCCCATGCGGTAGAGGAAAGATTAGACTGTGCTTTGGCAAATTAGGCTTGGTTCTAATTATTTCTGGAAGCATCATTAGAAAATTTAGTAGCTCCAGCTTCGGATCACTATCCTATTCTGTTGAACTGTACTCCTGTGGTGCGCCCTCATTTAGCAAAGCGCAACTTCCGGTTTGAAAATGCATGGAAACTAGAACCAAGATTTGATTATATGGTAAATGAGTGTTGGCAAACCACTTCAGAAGATGATATCCTCCCTCGTCAACAACGTTGTGCTGAGGAGATGGTAGTGTGGAGTAGAAACCATTGTAATAAGCTGAAAATTGAAATTGAGGAATGTCGTCATGAATTGAATCTTGCACGCAGTAGTCGCACTGGTGATGAGAAAAAACAGTTAGCACTGTTACACAAGCGCATGCACAGGTTGCTGGCTCAGGATGATGCTTATTGGAGACAATGA
Protein sequence

>MS.gene042027.t1

MSTSDLNLEGLTLSDEGLTLNLDVVTAETQILDHFLIGRVLTDKQVRFNYFKERMAYIWRPVKKVTIALDDTYRFLFQFNHCLDAMKVIKEAKDRGFMITTTWWLNILHPVQIDVTAPLKHEWQIRVNDGSYATICFKCEKLGTFCYLCGCLGHTDKMCSRRFDMEEDDGSRGWGEWLRSVIRCMGSASTNIWLQDPIPSRVQQDQQVPAAGFNGLHMGTSNSTSSVSSKKFGISLTDIASPSGTKASSLLPQMTGNNVQQPNQVVLALLAVSDENTGGNNYFAAAEFDSEAQGAELKNWKCAQTVEDKRAEDNPESVLESNVDGDKGMSLLAKRNFRFENAWKLEPRFDYMVNECWQTTSEDDILPRQQRCAEEMVVWSRNHCNKLKIEIEECRHELNLARSSRTGDEKKQLALLHKRMHRLLAQDDAYWRQ