AlfalfaGEDB | MS.gene41643

M. sativa cultivar XinJiangDaYe / MS.gene41643

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41643.t1	AES70414.1	53.8	346	93	4	1	345	1	280	3.00E-89	339

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41643.t1	O04244	27.7	137	93	3	83	216	6	139	3.4e-09	64.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41643.t1	G7J017	53.8	346	93	4	1	345	1	280	2.1e-89	339.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene021968	MS.gene41643	PPI
MS.gene41643	MS.gene012889	PPI
MS.gene09589	MS.gene41643	PPI
MS.gene41643	MS.gene000506	PPI
MS.gene43393	MS.gene41643	PPI
MS.gene057828	MS.gene41643	PPI
MS.gene41643	MS.gene028716	PPI
MS.gene41643	MS.gene74639	PPI
MS.gene41643	MS.gene071133	PPI
MS.gene41643	MS.gene60266	PPI
MS.gene41643	MS.gene51804	PPI
MS.gene41643	MS.gene013644	PPI
MS.gene41643	MS.gene54051	PPI
MS.gene41643	MS.gene22111	PPI
MS.gene66650	MS.gene41643	PPI
MS.gene41643	MS.gene049610	PPI
MS.gene005120	MS.gene41643	PPI
MS.gene41643	MS.gene37955	PPI
MS.gene048072	MS.gene41643	PPI
MS.gene060386	MS.gene41643	PPI
MS.gene71588	MS.gene41643	PPI
MS.gene41643	MS.gene010890	PPI
MS.gene04749	MS.gene41643	PPI
MS.gene05436	MS.gene41643	PPI
MS.gene059736	MS.gene41643	PPI
MS.gene70932	MS.gene41643	PPI
MS.gene42903	MS.gene41643	PPI
MS.gene81712	MS.gene41643	PPI
MS.gene41643	MS.gene060788	PPI
MS.gene021965	MS.gene41643	PPI
MS.gene055683	MS.gene41643	PPI
MS.gene042614	MS.gene41643	PPI
MS.gene41643	MS.gene77990	PPI
MS.gene26844	MS.gene41643	PPI
MS.gene021555	MS.gene41643	PPI
MS.gene41643	MS.gene062511	PPI
MS.gene048747	MS.gene41643	PPI
MS.gene41643	MS.gene027994	PPI
MS.gene021647	MS.gene41643	PPI
MS.gene41643	MS.gene007022	PPI
MS.gene41643	MS.gene69584	PPI
MS.gene41643	MS.gene52349	PPI
MS.gene41643	MS.gene021647	PPI
MS.gene009121	MS.gene41643	PPI
MS.gene41643	MS.gene46353	PPI
MS.gene94654	MS.gene41643	PPI
MS.gene51804	MS.gene41643	PPI
MS.gene36069	MS.gene41643	PPI
MS.gene41643	MS.gene61651	PPI
MS.gene97410	MS.gene41643	PPI
MS.gene83772	MS.gene41643	PPI
MS.gene32415	MS.gene41643	PPI
MS.gene035626	MS.gene41643	PPI
MS.gene008927	MS.gene41643	PPI
MS.gene045614	MS.gene41643	PPI
MS.gene41643	MS.gene016719	PPI
MS.gene41643	MS.gene88393	PPI
MS.gene41643	MS.gene054508	PPI
MS.gene63762	MS.gene41643	PPI
MS.gene41643	MS.gene053373	PPI
MS.gene71675	MS.gene41643	PPI
MS.gene30208	MS.gene41643	PPI
MS.gene41643	MS.gene63150	PPI
MS.gene013676	MS.gene41643	PPI
MS.gene41643	MS.gene072852	PPI
MS.gene030090	MS.gene41643	PPI
MS.gene95561	MS.gene41643	PPI
MS.gene41643	MS.gene65474	PPI
MS.gene96174	MS.gene41643	PPI
MS.gene41643	MS.gene025401	PPI
MS.gene018196	MS.gene41643	PPI
MS.gene41643	MS.gene08708	PPI
MS.gene065627	MS.gene41643	PPI
MS.gene41643	MS.gene06164	PPI
MS.gene41643	MS.gene50392	PPI
MS.gene41643	MS.gene22730	PPI
MS.gene57019	MS.gene41643	PPI
MS.gene41643	MS.gene032299	PPI
MS.gene41643	MS.gene017586	PPI
MS.gene018745	MS.gene41643	PPI
MS.gene65546	MS.gene41643	PPI
MS.gene41643	MS.gene037634	PPI
MS.gene41643	MS.gene057828	PPI
MS.gene41643	MS.gene08606	PPI
MS.gene03936	MS.gene41643	PPI
MS.gene038098	MS.gene41643	PPI
MS.gene41643	MS.gene000640	PPI
MS.gene60059	MS.gene41643	PPI
MS.gene41643	MS.gene98756	PPI
MS.gene34197	MS.gene41643	PPI
MS.gene28718	MS.gene41643	PPI
MS.gene41643	MS.gene98467	PPI
MS.gene055560	MS.gene41643	PPI
MS.gene41643	MS.gene62249	PPI
MS.gene008275	MS.gene41643	PPI
MS.gene41643	MS.gene024522	PPI
MS.gene038757	MS.gene41643	PPI
MS.gene064966	MS.gene41643	PPI
MS.gene41643	MS.gene010290	PPI
MS.gene41643	MS.gene92517	PPI
MS.gene41643	MS.gene045875	PPI
MS.gene41643	MS.gene84790	PPI
MS.gene41643	MS.gene044729	PPI
MS.gene41643	MS.gene60390	PPI
MS.gene41643	MS.gene039692	PPI
MS.gene43924	MS.gene41643	PPI
MS.gene046089	MS.gene41643	PPI
MS.gene047954	MS.gene41643	PPI
MS.gene35175	MS.gene41643	PPI
MS.gene41643	MS.gene55682	PPI
MS.gene41643	MS.gene002590	PPI
MS.gene036423	MS.gene41643	PPI
MS.gene013644	MS.gene41643	PPI
MS.gene72520	MS.gene41643	PPI
MS.gene66651	MS.gene41643	PPI
MS.gene41643	MS.gene059269	PPI
MS.gene96109	MS.gene41643	PPI
MS.gene41643	MS.gene057934	PPI
MS.gene35202	MS.gene41643	PPI
MS.gene41643	MS.gene024841	PPI
MS.gene051449	MS.gene41643	PPI
MS.gene41643	MS.gene069786	PPI
MS.gene63743	MS.gene41643	PPI
MS.gene41643	MS.gene025158	PPI
MS.gene41643	MS.gene40828	PPI
MS.gene48477	MS.gene41643	PPI
MS.gene045875	MS.gene41643	PPI
MS.gene41643	MS.gene51799	PPI
MS.gene30136	MS.gene41643	PPI
MS.gene008861	MS.gene41643	PPI
MS.gene61452	MS.gene41643	PPI
MS.gene41643	MS.gene40469	PPI
MS.gene016102	MS.gene41643	PPI
MS.gene46941	MS.gene41643	PPI
MS.gene96541	MS.gene41643	PPI
MS.gene41643	MS.gene09023	PPI
MS.gene000634	MS.gene41643	PPI
MS.gene41643	MS.gene025987	PPI
MS.gene85295	MS.gene41643	PPI
MS.gene41643	MS.gene95960	PPI
MS.gene41643	MS.gene043140	PPI
MS.gene47792	MS.gene41643	PPI
MS.gene46948	MS.gene41643	PPI
MS.gene98471	MS.gene41643	PPI
MS.gene41643	MS.gene03149	PPI
MS.gene025873	MS.gene41643	PPI
MS.gene012019	MS.gene41643	PPI
MS.gene05358	MS.gene41643	PPI
MS.gene41643	MS.gene29321	PPI
MS.gene93396	MS.gene41643	PPI
MS.gene074257	MS.gene41643	PPI
MS.gene41643	MS.gene015093	PPI
MS.gene05231	MS.gene41643	PPI
MS.gene41643	MS.gene24278	PPI
MS.gene41643	MS.gene000379	PPI
MS.gene41643	MS.gene34618	PPI
MS.gene010656	MS.gene41643	PPI
MS.gene000629	MS.gene41643	PPI
MS.gene41643	MS.gene59460	PPI
MS.gene041264	MS.gene41643	PPI
MS.gene41979	MS.gene41643	PPI
MS.gene41643	MS.gene060773	PPI
MS.gene41643	MS.gene66650	PPI
MS.gene05319	MS.gene41643	PPI
MS.gene41643	MS.gene08713	PPI
MS.gene56991	MS.gene41643	PPI
MS.gene050679	MS.gene41643	PPI
MS.gene41643	MS.gene09517	PPI
MS.gene41643	MS.gene87845	PPI
MS.gene20246	MS.gene41643	PPI
MS.gene055223	MS.gene41643	PPI
MS.gene28545	MS.gene41643	PPI
MS.gene41643	MS.gene24070	PPI
MS.gene053747	MS.gene41643	PPI
MS.gene41643	MS.gene26844	PPI
MS.gene41643	MS.gene64178	PPI
MS.gene65471	MS.gene41643	PPI
MS.gene061850	MS.gene41643	PPI
MS.gene41643	MS.gene074257	PPI
MS.gene41643	MS.gene073573	PPI
MS.gene41643	MS.gene44144	PPI
MS.gene41643	MS.gene060718	PPI
MS.gene073582	MS.gene41643	PPI
MS.gene058889	MS.gene41643	PPI
MS.gene98616	MS.gene41643	PPI
MS.gene40828	MS.gene41643	PPI
MS.gene41643	MS.gene027169	PPI
MS.gene41643	MS.gene004417	PPI
MS.gene41643	MS.gene019177	PPI
MS.gene042027	MS.gene41643	PPI
MS.gene69490	MS.gene41643	PPI
MS.gene015248	MS.gene41643	PPI
MS.gene80362	MS.gene41643	PPI
MS.gene44083	MS.gene41643	PPI
MS.gene41643	MS.gene055560	PPI
MS.gene41643	MS.gene009213	PPI
MS.gene013193	MS.gene41643	PPI
MS.gene41643	MS.gene024297	PPI
MS.gene04970	MS.gene41643	PPI
MS.gene08106	MS.gene41643	PPI
MS.gene41643	MS.gene003264	PPI
MS.gene41643	MS.gene054652	PPI
MS.gene41643	MS.gene37537	PPI
MS.gene41643	MS.gene42010	PPI
MS.gene41643	MS.gene27329	PPI
MS.gene23521	MS.gene41643	PPI
MS.gene41643	MS.gene41393	PPI
MS.gene41643	MS.gene89815	PPI
MS.gene41643	MS.gene016632	PPI
MS.gene41643	MS.gene018474	PPI
MS.gene41643	MS.gene86868	PPI
MS.gene87529	MS.gene41643	PPI
MS.gene024405	MS.gene41643	PPI
MS.gene012748	MS.gene41643	PPI
MS.gene04134	MS.gene41643	PPI
MS.gene83979	MS.gene41643	PPI
MS.gene062067	MS.gene41643	PPI
MS.gene41643	MS.gene058889	PPI
MS.gene41643	MS.gene96163	PPI
MS.gene57044	MS.gene41643	PPI
MS.gene049610	MS.gene41643	PPI
MS.gene017586	MS.gene41643	PPI
MS.gene038676	MS.gene41643	PPI
MS.gene71637	MS.gene41643	PPI
MS.gene41643	MS.gene34463	PPI
MS.gene042651	MS.gene41643	PPI
MS.gene05576	MS.gene41643	PPI
MS.gene41643	MS.gene037049	PPI
MS.gene41643	MS.gene61499	PPI
MS.gene98467	MS.gene41643	PPI
MS.gene037049	MS.gene41643	PPI
MS.gene41643	MS.gene019165	PPI
MS.gene034643	MS.gene41643	PPI
MS.gene41643	MS.gene81194	PPI
MS.gene64130	MS.gene41643	PPI
MS.gene41643	MS.gene038394	PPI
MS.gene41643	MS.gene019001	PPI
MS.gene41643	MS.gene53904	PPI
MS.gene41643	MS.gene73261	PPI
MS.gene071784	MS.gene41643	PPI
MS.gene41643	MS.gene85538	PPI
MS.gene41643	MS.gene025675	PPI
MS.gene061579	MS.gene41643	PPI
MS.gene41643	MS.gene055546	PPI
MS.gene76420	MS.gene41643	PPI
MS.gene071320	MS.gene41643	PPI
MS.gene41643	MS.gene55209	PPI
MS.gene026680	MS.gene41643	PPI
MS.gene26433	MS.gene41643	PPI
MS.gene41643	MS.gene022929	PPI
MS.gene042559	MS.gene41643	PPI
MS.gene22200	MS.gene41643	PPI
MS.gene41643	MS.gene068629	PPI
MS.gene26038	MS.gene41643	PPI
MS.gene41643	MS.gene009781	PPI
MS.gene85308	MS.gene41643	PPI
MS.gene41643	MS.gene048342	PPI
MS.gene031967	MS.gene41643	PPI
MS.gene41643	MS.gene069624	PPI
MS.gene34812	MS.gene41643	PPI
MS.gene02896	MS.gene41643	PPI
MS.gene41643	MS.gene21066	PPI
MS.gene41643	MS.gene013890	PPI
MS.gene063214	MS.gene41643	PPI
MS.gene55682	MS.gene41643	PPI
MS.gene41643	MS.gene98127	PPI
MS.gene41643	MS.gene073582	PPI
MS.gene58419	MS.gene41643	PPI
MS.gene41643	MS.gene028714	PPI
MS.gene067340	MS.gene41643	PPI
MS.gene41643	MS.gene048706	PPI
MS.gene41643	MS.gene69586	PPI
MS.gene41643	MS.gene45083	PPI
MS.gene65623	MS.gene41643	PPI
MS.gene41643	MS.gene049561	PPI
MS.gene41643	MS.gene008658	PPI
MS.gene69495	MS.gene41643	PPI
MS.gene049512	MS.gene41643	PPI
MS.gene41643	MS.gene49057	PPI
MS.gene29406	MS.gene41643	PPI
MS.gene038623	MS.gene41643	PPI
MS.gene86275	MS.gene41643	PPI
MS.gene05357	MS.gene41643	PPI
MS.gene41643	MS.gene048088	PPI
MS.gene032311	MS.gene41643	PPI
MS.gene86849	MS.gene41643	PPI
MS.gene41643	MS.gene86275	PPI
MS.gene072852	MS.gene41643	PPI
MS.gene39956	MS.gene41643	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41643.t1	MTR_3g053920	52.722	349	92	5	1	345	1	280	1.76e-112	330
MS.gene41643.t1	MTR_2g061500	75.969	129	31	0	1	129	1	129	6.68e-69	213
MS.gene41643.t1	MTR_4g103530	73.196	97	26	0	55	151	1	97	1.05e-45	155
MS.gene41643.t1	MTR_7g011710	74.118	85	22	0	55	139	1	85	2.21e-39	138
MS.gene41643.t1	MTR_6g033215	35.838	173	109	1	29	199	122	294	2.76e-36	134
MS.gene41643.t1	MTR_0010s0370	36.571	175	105	5	8	177	4	177	8.62e-30	114
MS.gene41643.t1	MTR_0010s0350	36.571	175	105	5	8	177	4	177	8.62e-30	114
MS.gene41643.t1	MTR_8g463110	56.122	98	35	2	289	385	1	91	1.08e-15	79.3
MS.gene41643.t1	MTR_1g036720	31.731	104	69	1	11	112	7	110	5.39e-13	65.5
MS.gene41643.t1	MTR_4g020545	34.483	116	60	3	63	178	79	178	9.18e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41643.t1	AT2G17920	25.701	214	153	3	7	216	8	219	3.29e-17	82.0
MS.gene41643.t1	AT5G36228	26.904	197	137	3	36	227	39	233	1.78e-16	80.5
MS.gene41643.t1	AT3G31430	28.108	185	121	5	36	215	134	311	2.80e-15	76.6
MS.gene41643.t1	AT2G41590	28.472	144	95	3	77	216	80	219	1.02e-14	75.5
MS.gene41643.t1	AT2G13450	26.776	183	128	3	36	215	39	218	1.84e-14	74.7
MS.gene41643.t1	AT3G57587	26.056	142	101	2	77	216	80	219	3.06e-14	73.9
MS.gene41643.t1	AT3G47920	25.352	142	102	2	77	216	9	148	5.18e-12	66.6

Find 103 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTAATTCATTTGGATATC+TGG	0.113881	3.4:-88467241	MS.gene41643:CDS
CTGCTTGATCGGTCGTGTTT+TGG	0.188329	3.4:-88467486	MS.gene41643:CDS
CGGACGATGGAGTACGTAAT+TGG	0.209232	3.4:-88466920	MS.gene41643:CDS
CACTGTTCCTCTAATTCATT+TGG	0.259561	3.4:-88467249	MS.gene41643:CDS
GATATCTGGGTGCAGGTCTA+TGG	0.275435	3.4:-88467227	MS.gene41643:CDS
CAGATTCAGCAGATAGGTTA+AGG	0.317540	3.4:+88467531	None:intergenic
TTTACTGCAGGGTCGTTCTA+TGG	0.328442	3.4:-88466577	MS.gene41643:CDS
AAACCTGTTACGACTCGTAT+AGG	0.344071	3.4:-88466885	MS.gene41643:CDS
CCTATATGCGCCTCAAAGTC+TGG	0.351054	3.4:-88467106	MS.gene41643:CDS
CAGGGTCGTTCTATGGTACT+TGG	0.351644	3.4:-88466570	MS.gene41643:CDS
CAAGGCATTGGCAGCTTTCT+CGG	0.355349	3.4:-88467170	MS.gene41643:CDS
TGTCACTGTACCGCTGAAAA+AGG	0.360408	3.4:-88467078	MS.gene41643:CDS
GGACGATGGAGTACGTAATT+GGG	0.361200	3.4:-88466919	MS.gene41643:CDS
CTTTGATGCGGCACAACATC+AGG	0.362158	3.4:+88466825	None:intergenic
CAGGTCTATGGATTGCCCTT+TGG	0.363802	3.4:-88467215	MS.gene41643:CDS
GACCCTATACGAGTCGTAAC+AGG	0.367774	3.4:+88466882	None:intergenic
CAGATATCCAAATGAATTAG+AGG	0.368589	3.4:+88467242	None:intergenic
TGCTACAAACAGATGGCTTC+AGG	0.376139	3.4:-88466856	MS.gene41643:CDS
TCTAATTCATTTGGATATCT+GGG	0.377178	3.4:-88467240	MS.gene41643:CDS
AATGGTGTTGCGACCATCAT+AGG	0.380913	3.4:+88467136	None:intergenic
TGCTATTAATGCTATTAAAC+AGG	0.387604	3.4:-88466706	MS.gene41643:CDS
TGTTACAGGTGTGGCATTCT+CGG	0.402641	3.4:-88466984	MS.gene41643:CDS
CATCAAGCTTGTGGTGGAAC+TGG	0.403105	3.4:+88467357	None:intergenic
AACCTGTTACGACTCGTATA+GGG	0.403226	3.4:-88466884	MS.gene41643:CDS
GTGTCCCGACTTGTTTGAAT+TGG	0.427535	3.4:-88466946	MS.gene41643:CDS
CTCGGCCTCAAATTTACTGC+AGG	0.434485	3.4:-88466589	MS.gene41643:CDS
GGTGCGGCCAGCAGAAGAAC+TGG	0.435350	3.4:+88466791	None:intergenic
AGGTTTCAGATTCAGCAGAT+AGG	0.439470	3.4:+88467525	None:intergenic
AGATTCAGCAGATAGGTTAA+GGG	0.441257	3.4:+88467532	None:intergenic
GAGGCCGAGACTCCTGTAGA+TGG	0.454282	3.4:+88466603	None:intergenic
ATGTCCCGTGCGTGGAAACC+TGG	0.455231	3.4:-88467425	MS.gene41643:CDS
GACCGATCAAGCAGTGATCT+AGG	0.458613	3.4:+88467495	None:intergenic
CTTTGGTTTCATTCAATCAA+AGG	0.459068	3.4:-88467198	MS.gene41643:CDS
GGAGGACTTTCCATTCTAAA+GGG	0.459478	3.4:+88466437	None:intergenic
CCAGACTTTGAGGCGCATAT+AGG	0.464440	3.4:+88467106	None:intergenic
GTGTGGCATTCTCGGACATT+CGG	0.466234	3.4:-88466976	MS.gene41643:CDS
ATAATAGTTACGCGTTTGCC+AGG	0.478326	3.4:+88467407	None:intergenic
GCAAAAGTGAAAGGAGCAGC+AGG	0.480319	3.4:+88466543	None:intergenic
GTATCCTTGATGAGGGTCCT+TGG	0.481984	3.4:-88467328	MS.gene41643:CDS
CGACTTGTTTGAATTGGACT+CGG	0.483849	3.4:-88466940	MS.gene41643:CDS
TCATTTGGATATCTGGGTGC+AGG	0.490055	3.4:-88467234	MS.gene41643:CDS
GTTTGCCAGGTTTCCACGCA+CGG	0.490364	3.4:+88467420	None:intergenic
TTCTCCATCTACAGGAGTCT+CGG	0.493364	3.4:-88466607	MS.gene41643:CDS
ATGCTTCCTTCTCCATCTAC+AGG	0.496559	3.4:-88466615	MS.gene41643:CDS
TTCCAAGCCAGTTCTTCTGC+TGG	0.498549	3.4:-88466798	MS.gene41643:CDS
GGTTTCATTCAATCAAAGGT+TGG	0.507457	3.4:-88467194	MS.gene41643:CDS
TGTTGCGACCATCATAGGTT+AGG	0.510688	3.4:+88467141	None:intergenic
GTCATGGAACGCATTGCTCC+TGG	0.512312	3.4:-88467284	MS.gene41643:CDS
TCAAAGGTTGGTCAAGGCAT+TGG	0.512470	3.4:-88467182	MS.gene41643:CDS
TTTGATTGAATGAAACCAAA+GGG	0.513269	3.4:+88467200	None:intergenic
TTTGGACGATCGTGAAATCA+AGG	0.514839	3.4:-88467468	MS.gene41643:CDS
TTTGAATTGGACTCGGACGA+TGG	0.520707	3.4:-88466933	MS.gene41643:CDS
ACGACCCTGCAGTAAATTTG+AGG	0.521272	3.4:+88466584	None:intergenic
TGACATCAATCCAGACTTTG+AGG	0.530919	3.4:+88467096	None:intergenic
CAACATTGACGGTCTTACCT+TGG	0.534894	3.4:-88467570	MS.gene41643:CDS
ATTCAATCAAAGGTTGGTCA+AGG	0.545909	3.4:-88467188	MS.gene41643:CDS
GAGTCCAATTCAAACAAGTC+GGG	0.547426	3.4:+88466942	None:intergenic
GTTGAGTTGTGTGGGATCCA+TGG	0.548620	3.4:+88467589	None:intergenic
TGAATTAGAGGAACAGTGAT+CGG	0.549035	3.4:+88467254	None:intergenic
CCCACACAACTCAACATTGA+CGG	0.549913	3.4:-88467581	MS.gene41643:CDS
TCAAAACAGAATTGTCAGCA+TGG	0.555985	3.4:+88466769	None:intergenic
ATGCGGCACAACATCAGGGA+TGG	0.556865	3.4:+88466830	None:intergenic
ATCCTAGATCACTGCTTGAT+CGG	0.559464	3.4:-88467497	MS.gene41643:CDS
TTTGTACCAGCAAAAGTGAA+AGG	0.560500	3.4:+88466534	None:intergenic
CAGAATTGTCAGCATGGGTG+CGG	0.561489	3.4:+88466775	None:intergenic
GGTACTCTCCTAACCTATGA+TGG	0.562210	3.4:-88467149	MS.gene41643:CDS
GGCCAGCAGAAGAACTGGCT+TGG	0.565580	3.4:+88466796	None:intergenic
TGCCGCTCGTATCCTTGATG+AGG	0.569722	3.4:-88467336	MS.gene41643:CDS
CAAAACAGAATTGTCAGCAT+GGG	0.573225	3.4:+88466770	None:intergenic
ACAGTGATCGGAACCACACC+AGG	0.573710	3.4:+88467266	None:intergenic
TTTGCCAGGTTTCCACGCAC+GGG	0.575795	3.4:+88467421	None:intergenic
GGTCTTACCTTGGCTGACGA+AGG	0.583933	3.4:-88467560	MS.gene41643:CDS
GCCGCTCGTATCCTTGATGA+GGG	0.585959	3.4:-88467335	MS.gene41643:CDS
GGACATTCGTTCACTGAAGT+AGG	0.587017	3.4:+88467442	None:intergenic
GGTCAAACCTTCGTCAGCCA+AGG	0.587526	3.4:+88467553	None:intergenic
TACGAGCGGCATCAAGCTTG+TGG	0.589023	3.4:+88467348	None:intergenic
CAGTGTCTACAGCAAGCATA+CGG	0.590441	3.4:+88466727	None:intergenic
TGCGGCACAACATCAGGGAT+GGG	0.590978	3.4:+88466831	None:intergenic
CATTCTCGGACATTCGGACA+AGG	0.591084	3.4:-88466970	MS.gene41643:CDS
TGATAACTTTCATATAGTCA+TGG	0.592825	3.4:-88467300	MS.gene41643:CDS
GTGAACGAATGTCCCGTGCG+TGG	0.593922	3.4:-88467433	MS.gene41643:CDS
GCTTGGAAGTTACTTTGATG+CGG	0.594151	3.4:+88466813	None:intergenic
GAGACTCCTGTAGATGGAGA+AGG	0.602523	3.4:+88466609	None:intergenic
ACCGTCAATGTTGAGTTGTG+TGG	0.602644	3.4:+88467580	None:intergenic
TGGAAAGTTCGTGCTAGCAA+TGG	0.606670	3.4:-88467053	MS.gene41643:CDS
CTTTGATTGAATGAAACCAA+AGG	0.608719	3.4:+88467199	None:intergenic
CGAGTCCAATTCAAACAAGT+CGG	0.610252	3.4:+88466941	None:intergenic
GAGCGGCATCAAGCTTGTGG+TGG	0.615991	3.4:+88467351	None:intergenic
GACGATGGAGTACGTAATTG+GGG	0.621097	3.4:-88466918	MS.gene41643:CDS
GGTCGGCTGCTACAAACAGA+TGG	0.622085	3.4:-88466863	MS.gene41643:CDS
CCGTCAATGTTGAGTTGTGT+GGG	0.623706	3.4:+88467581	None:intergenic
ATAACCAAGGACCCTCATCA+AGG	0.624768	3.4:+88467324	None:intergenic
GCGCATATAGGAGCTATGAA+TGG	0.625349	3.4:+88467118	None:intergenic
TTTGATGCGGCACAACATCA+GGG	0.625918	3.4:+88466826	None:intergenic
GTAGATGGAGAAGGAAGCAT+CGG	0.627617	3.4:+88466618	None:intergenic
ATCAAGCAGTGATCTAGGAT+AGG	0.632523	3.4:+88467500	None:intergenic
TCGGCCTCAAATTTACTGCA+GGG	0.638651	3.4:-88466588	MS.gene41643:CDS
GGAACGCATTGCTCCTGGTG+TGG	0.640570	3.4:-88467279	MS.gene41643:CDS
GCAGAGCAGTCTGAGCAGCG+AGG	0.684222	3.4:+88466679	None:intergenic
ACCCTCATCAAGGATACGAG+CGG	0.685761	3.4:+88467334	None:intergenic
TGAAAGTTATCATATAACCA+AGG	0.688479	3.4:+88467311	None:intergenic
GCAGTGATCTAGGATAGGAG+AGG	0.689001	3.4:+88467505	None:intergenic
TGTTACGACTCGTATAGGGT+CGG	0.713178	3.4:-88466880	MS.gene41643:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAAAAAAATAGTAACAA+AGG	+	chr3.4:88467014-88467033	None:intergenic	15.0%
!!!	TTTTTCAAATATGAGAAACT+GGG	-	chr3.4:88467025-88467044	MS.gene41643:CDS	20.0%
!!!	TTTTTTCAAATATGAGAAAC+TGG	-	chr3.4:88467024-88467043	MS.gene41643:CDS	20.0%
!	TCTAATTCATTTGGATATCT+GGG	-	chr3.4:88466799-88466818	MS.gene41643:CDS	25.0%
!	TGAAAGTTATCATATAACCA+AGG	+	chr3.4:88466731-88466750	None:intergenic	25.0%
!	TGATAACTTTCATATAGTCA+TGG	-	chr3.4:88466739-88466758	MS.gene41643:CDS	25.0%
!	TGCTATTAATGCTATTAAAC+AGG	-	chr3.4:88467333-88467352	MS.gene41643:CDS	25.0%
!	TTTGATTGAATGAAACCAAA+GGG	+	chr3.4:88466842-88466861	None:intergenic	25.0%
	CAAGCTAAAAATGTCACAAA+TGG	-	chr3.4:88467382-88467401	MS.gene41643:CDS	30.0%
	CAGATATCCAAATGAATTAG+AGG	+	chr3.4:88466800-88466819	None:intergenic	30.0%
	CTCTAATTCATTTGGATATC+TGG	-	chr3.4:88466798-88466817	MS.gene41643:CDS	30.0%
	CTTTGATTGAATGAAACCAA+AGG	+	chr3.4:88466843-88466862	None:intergenic	30.0%
!	CTTTGGTTTCATTCAATCAA+AGG	-	chr3.4:88466841-88466860	MS.gene41643:CDS	30.0%
	AAAAATGTCACAAATGGTGC+TGG	-	chr3.4:88467388-88467407	MS.gene41643:CDS	35.0%
	AGATTCAGCAGATAGGTTAA+GGG	+	chr3.4:88466510-88466529	None:intergenic	35.0%
	CAAAACAGAATTGTCAGCAT+GGG	+	chr3.4:88467272-88467291	None:intergenic	35.0%
	CACTGTTCCTCTAATTCATT+TGG	-	chr3.4:88466790-88466809	MS.gene41643:CDS	35.0%
	GGTTTCATTCAATCAAAGGT+TGG	-	chr3.4:88466845-88466864	MS.gene41643:CDS	35.0%
	TCAAAACAGAATTGTCAGCA+TGG	+	chr3.4:88467273-88467292	None:intergenic	35.0%
	TGAATTAGAGGAACAGTGAT+CGG	+	chr3.4:88466788-88466807	None:intergenic	35.0%
	TTTGTACCAGCAAAAGTGAA+AGG	+	chr3.4:88467508-88467527	None:intergenic	35.0%
!	ATTCAATCAAAGGTTGGTCA+AGG	-	chr3.4:88466851-88466870	MS.gene41643:CDS	35.0%
!!!	AACTGGGTGTTTTTTGTTAC+AGG	-	chr3.4:88467041-88467060	MS.gene41643:CDS	35.0%
	AAACCTGTTACGACTCGTAT+AGG	-	chr3.4:88467154-88467173	MS.gene41643:CDS	40.0%
	AACCTGTTACGACTCGTATA+GGG	-	chr3.4:88467155-88467174	MS.gene41643:CDS	40.0%
	AGGTTTCAGATTCAGCAGAT+AGG	+	chr3.4:88466517-88466536	None:intergenic	40.0%
	ATAATAGTTACGCGTTTGCC+AGG	+	chr3.4:88466635-88466654	None:intergenic	40.0%
	ATCCTAGATCACTGCTTGAT+CGG	-	chr3.4:88466542-88466561	MS.gene41643:CDS	40.0%
	CAGATTCAGCAGATAGGTTA+AGG	+	chr3.4:88466511-88466530	None:intergenic	40.0%
	CGAGTCCAATTCAAACAAGT+CGG	+	chr3.4:88467101-88467120	None:intergenic	40.0%
	GAGTCCAATTCAAACAAGTC+GGG	+	chr3.4:88467100-88467119	None:intergenic	40.0%
	TGACATCAATCCAGACTTTG+AGG	+	chr3.4:88466946-88466965	None:intergenic	40.0%
	TTTGGACGATCGTGAAATCA+AGG	-	chr3.4:88466571-88466590	MS.gene41643:CDS	40.0%
!	ATCAAGCAGTGATCTAGGAT+AGG	+	chr3.4:88466542-88466561	None:intergenic	40.0%
!	CGACTTGTTTGAATTGGACT+CGG	-	chr3.4:88467099-88467118	MS.gene41643:CDS	40.0%
!	GCTTGGAAGTTACTTTGATG+CGG	+	chr3.4:88467229-88467248	None:intergenic	40.0%
!!!	ACTTTCCACTCCTTTTTCAG+CGG	+	chr3.4:88466974-88466993	None:intergenic	40.0%
!!!	CGACAACGTTGTTTTTCTTG+AGG	-	chr3.4:88467540-88467559	MS.gene41643:CDS	40.0%
!!!	GGTGTTTTTTGTTACAGGTG+TGG	-	chr3.4:88467046-88467065	MS.gene41643:CDS	40.0%
!!!	GTTGTTTTTCTTGAGGATGC+TGG	-	chr3.4:88467547-88467566	MS.gene41643:CDS	40.0%
	ACGACCCTGCAGTAAATTTG+AGG	+	chr3.4:88467458-88467477	None:intergenic	45.0%
	ATAACCAAGGACCCTCATCA+AGG	+	chr3.4:88466718-88466737	None:intergenic	45.0%
	ATGCTTCCTTCTCCATCTAC+AGG	-	chr3.4:88467424-88467443	MS.gene41643:CDS	45.0%
	CAACATTGACGGTCTTACCT+TGG	-	chr3.4:88466469-88466488	MS.gene41643:CDS	45.0%
	CAGTGTCTACAGCAAGCATA+CGG	+	chr3.4:88467315-88467334	None:intergenic	45.0%
	CCCACACAACTCAACATTGA+CGG	-	chr3.4:88466458-88466477	MS.gene41643:CDS	45.0%
	GACGATGGAGTACGTAATTG+GGG	-	chr3.4:88467121-88467140	MS.gene41643:CDS	45.0%
	GCGCATATAGGAGCTATGAA+TGG	+	chr3.4:88466924-88466943	None:intergenic	45.0%
	GGACATTCGTTCACTGAAGT+AGG	+	chr3.4:88466600-88466619	None:intergenic	45.0%
	GGACGATGGAGTACGTAATT+GGG	-	chr3.4:88467120-88467139	MS.gene41643:CDS	45.0%
	GGTACTCTCCTAACCTATGA+TGG	-	chr3.4:88466890-88466909	MS.gene41643:CDS	45.0%
	TCATTTGGATATCTGGGTGC+AGG	-	chr3.4:88466805-88466824	MS.gene41643:CDS	45.0%
	TCGGCCTCAAATTTACTGCA+GGG	-	chr3.4:88467451-88467470	MS.gene41643:CDS	45.0%
	TGCTACAAACAGATGGCTTC+AGG	-	chr3.4:88467183-88467202	MS.gene41643:CDS	45.0%
	TGGAAAGTTCGTGCTAGCAA+TGG	-	chr3.4:88466986-88467005	MS.gene41643:CDS	45.0%
	TGTCACTGTACCGCTGAAAA+AGG	-	chr3.4:88466961-88466980	MS.gene41643:CDS	45.0%
	TGTTACAGGTGTGGCATTCT+CGG	-	chr3.4:88467055-88467074	MS.gene41643:CDS	45.0%
	TGTTACGACTCGTATAGGGT+CGG	-	chr3.4:88467159-88467178	MS.gene41643:CDS	45.0%
	TGTTGCGACCATCATAGGTT+AGG	+	chr3.4:88466901-88466920	None:intergenic	45.0%
	TTCTCCATCTACAGGAGTCT+CGG	-	chr3.4:88467432-88467451	MS.gene41643:CDS	45.0%
	TTTACTGCAGGGTCGTTCTA+TGG	-	chr3.4:88467462-88467481	MS.gene41643:CDS	45.0%
	TTTGAATTGGACTCGGACGA+TGG	-	chr3.4:88467106-88467125	MS.gene41643:CDS	45.0%
	TTTGATGCGGCACAACATCA+GGG	+	chr3.4:88467216-88467235	None:intergenic	45.0%
!	ACCGTCAATGTTGAGTTGTG+TGG	+	chr3.4:88466462-88466481	None:intergenic	45.0%
!	CCGTCAATGTTGAGTTGTGT+GGG	+	chr3.4:88466461-88466480	None:intergenic	45.0%
!	GTAGATGGAGAAGGAAGCAT+CGG	+	chr3.4:88467424-88467443	None:intergenic	45.0%
!	GTGTCCCGACTTGTTTGAAT+TGG	-	chr3.4:88467093-88467112	MS.gene41643:CDS	45.0%
!	TCAAAGGTTGGTCAAGGCAT+TGG	-	chr3.4:88466857-88466876	MS.gene41643:CDS	45.0%
!!	AATGGTGTTGCGACCATCAT+AGG	+	chr3.4:88466906-88466925	None:intergenic	45.0%
	ACCCTCATCAAGGATACGAG+CGG	+	chr3.4:88466708-88466727	None:intergenic	50.0%
	CAGAATTGTCAGCATGGGTG+CGG	+	chr3.4:88467267-88467286	None:intergenic	50.0%
	CAGGGTCGTTCTATGGTACT+TGG	-	chr3.4:88467469-88467488	MS.gene41643:CDS	50.0%
	CATTCTCGGACATTCGGACA+AGG	-	chr3.4:88467069-88467088	MS.gene41643:CDS	50.0%
	CCTATATGCGCCTCAAAGTC+TGG	-	chr3.4:88466933-88466952	MS.gene41643:CDS	50.0%
	CGGACGATGGAGTACGTAAT+TGG	-	chr3.4:88467119-88467138	MS.gene41643:CDS	50.0%
	CTCGGCCTCAAATTTACTGC+AGG	-	chr3.4:88467450-88467469	MS.gene41643:CDS	50.0%
	CTGTACCGCTGAAAAAGGAG+TGG	-	chr3.4:88466966-88466985	MS.gene41643:CDS	50.0%
	CTTTGATGCGGCACAACATC+AGG	+	chr3.4:88467217-88467236	None:intergenic	50.0%
	GACCCTATACGAGTCGTAAC+AGG	+	chr3.4:88467160-88467179	None:intergenic	50.0%
	GACCGATCAAGCAGTGATCT+AGG	+	chr3.4:88466547-88466566	None:intergenic	50.0%
	GAGACTCCTGTAGATGGAGA+AGG	+	chr3.4:88467433-88467452	None:intergenic	50.0%
	GATATCTGGGTGCAGGTCTA+TGG	-	chr3.4:88466812-88466831	MS.gene41643:CDS	50.0%
	GCAAAAGTGAAAGGAGCAGC+AGG	+	chr3.4:88467499-88467518	None:intergenic	50.0%
	GTATCCTTGATGAGGGTCCT+TGG	-	chr3.4:88466711-88466730	MS.gene41643:CDS	50.0%
	GTGTGGCATTCTCGGACATT+CGG	-	chr3.4:88467063-88467082	MS.gene41643:CDS	50.0%
	TTCCAAGCCAGTTCTTCTGC+TGG	-	chr3.4:88467241-88467260	MS.gene41643:CDS	50.0%
!	CAGGTCTATGGATTGCCCTT+TGG	-	chr3.4:88466824-88466843	MS.gene41643:CDS	50.0%
!	CATCAAGCTTGTGGTGGAAC+TGG	+	chr3.4:88466685-88466704	None:intergenic	50.0%
!	CTGCTTGATCGGTCGTGTTT+TGG	-	chr3.4:88466553-88466572	MS.gene41643:CDS	50.0%
!	GCAGTGATCTAGGATAGGAG+AGG	+	chr3.4:88466537-88466556	None:intergenic	50.0%
!!	CAAGGCATTGGCAGCTTTCT+CGG	-	chr3.4:88466869-88466888	MS.gene41643:CDS	50.0%
!!	CCAGACTTTGAGGCGCATAT+AGG	+	chr3.4:88466936-88466955	None:intergenic	50.0%
!!	GCTGCTCCTTTCACTTTTGC+TGG	-	chr3.4:88467499-88467518	MS.gene41643:CDS	50.0%
	ACAGTGATCGGAACCACACC+AGG	+	chr3.4:88466776-88466795	None:intergenic	55.0%
	ATGCGGCACAACATCAGGGA+TGG	+	chr3.4:88467212-88467231	None:intergenic	55.0%
	GCCGCTCGTATCCTTGATGA+GGG	-	chr3.4:88466704-88466723	MS.gene41643:CDS	55.0%
	GGTCAAACCTTCGTCAGCCA+AGG	+	chr3.4:88466489-88466508	None:intergenic	55.0%
	GGTCGGCTGCTACAAACAGA+TGG	-	chr3.4:88467176-88467195	MS.gene41643:CDS	55.0%
	GGTCTTACCTTGGCTGACGA+AGG	-	chr3.4:88466479-88466498	MS.gene41643:CDS	55.0%
	GTCATGGAACGCATTGCTCC+TGG	-	chr3.4:88466755-88466774	MS.gene41643:CDS	55.0%
	GTTTGCCAGGTTTCCACGCA+CGG	+	chr3.4:88466622-88466641	None:intergenic	55.0%
	TACGAGCGGCATCAAGCTTG+TGG	+	chr3.4:88466694-88466713	None:intergenic	55.0%
	TGCCGCTCGTATCCTTGATG+AGG	-	chr3.4:88466703-88466722	MS.gene41643:CDS	55.0%
	TGCGGCACAACATCAGGGAT+GGG	+	chr3.4:88467211-88467230	None:intergenic	55.0%
	TTTGCCAGGTTTCCACGCAC+GGG	+	chr3.4:88466621-88466640	None:intergenic	55.0%
	ATGTCCCGTGCGTGGAAACC+TGG	-	chr3.4:88466614-88466633	MS.gene41643:CDS	60.0%
	GAGCGGCATCAAGCTTGTGG+TGG	+	chr3.4:88466691-88466710	None:intergenic	60.0%
	GAGGCCGAGACTCCTGTAGA+TGG	+	chr3.4:88467439-88467458	None:intergenic	60.0%
	GGAACGCATTGCTCCTGGTG+TGG	-	chr3.4:88466760-88466779	MS.gene41643:CDS	60.0%
	GGCCAGCAGAAGAACTGGCT+TGG	+	chr3.4:88467246-88467265	None:intergenic	60.0%
	GTGAACGAATGTCCCGTGCG+TGG	-	chr3.4:88466606-88466625	MS.gene41643:CDS	60.0%
	GCAGAGCAGTCTGAGCAGCG+AGG	+	chr3.4:88467363-88467382	None:intergenic	65.0%
	GGTGCGGCCAGCAGAAGAAC+TGG	+	chr3.4:88467251-88467270	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	88466452	88467609	88466452	ID=MS.gene41643
chr3.4	mRNA	88466452	88467609	88466452	ID=MS.gene41643.t1;Parent=MS.gene41643
chr3.4	exon	88466452	88467609	88466452	ID=MS.gene41643.t1.exon1;Parent=MS.gene41643.t1
chr3.4	CDS	88466452	88467609	88466452	ID=cds.MS.gene41643.t1;Parent=MS.gene41643.t1

Gene Sequence

>MS.gene41643

ATGGATCCCACACAACTCAACATTGACGGTCTTACCTTGGCTGACGAAGGTTTGACCCTTAACCTATCTGCTGAATCTGAAACCTCTCCTATCCTAGATCACTGCTTGATCGGTCGTGTTTTGGACGATCGTGAAATCAAGGCAGCCTACTTCAGTGAACGAATGTCCCGTGCGTGGAAACCTGGCAAACGCGTAACTATTATCAAATCTGATGATGATCGCTTCTTGTTCCAGTTCCACCACAAGCTTGATGCCGCTCGTATCCTTGATGAGGGTCCTTGGTTATATGATAACTTTCATATAGTCATGGAACGCATTGCTCCTGGTGTGGTTCCGATCACTGTTCCTCTAATTCATTTGGATATCTGGGTGCAGGTCTATGGATTGCCCTTTGGTTTCATTCAATCAAAGGTTGGTCAAGGCATTGGCAGCTTTCTCGGTACTCTCCTAACCTATGATGGTCGCAACACCATTCATAGCTCCTATATGCGCCTCAAAGTCTGGATTGATGTCACTGTACCGCTGAAAAAGGAGTGGAAAGTTCGTGCTAGCAATGGTTCCTTTGTTACTATTTTTTTCAAATATGAGAAACTGGGTGTTTTTTGTTACAGGTGTGGCATTCTCGGACATTCGGACAAGGTGTGTCCCGACTTGTTTGAATTGGACTCGGACGATGGAGTACGTAATTGGGGACTTGATCTCAAACCTGTTACGACTCGTATAGGGTCGGCTGCTACAAACAGATGGCTTCAGGATCCCATCCCTGATGTTGTGCCGCATCAAAGTAACTTCCAAGCCAGTTCTTCTGCTGGCCGCACCCATGCTGACAATTCTGTTTTGAATTTTAATAACTTTAATAACCGTATGCTTGCTGTAGACACTGCTATTAATGCTATTAAACAGGATGTCCTCGCTGCTCAGACTGCTCTGCAAGCTAAAAATGTCACAAATGGTGCTGGTTGTCAAGTTCCGATGCTTCCTTCTCCATCTACAGGAGTCTCGGCCTCAAATTTACTGCAGGGTCGTTCTATGGTACTTGGTTTGCCTGCTGCTCCTTTCACTTTTGCTGGTACAAATAATACTCAAAGCGACAACGTTGTTTTTCTTGAGGATGCTGGTTCTGACTTGAAGAAACGCAAGTGTATGCTCGCCCTTTAG

Protein sequence

>MS.gene41643.t1

MDPTQLNIDGLTLADEGLTLNLSAESETSPILDHCLIGRVLDDREIKAAYFSERMSRAWKPGKRVTIIKSDDDRFLFQFHHKLDAARILDEGPWLYDNFHIVMERIAPGVVPITVPLIHLDIWVQVYGLPFGFIQSKVGQGIGSFLGTLLTYDGRNTIHSSYMRLKVWIDVTVPLKKEWKVRASNGSFVTIFFKYEKLGVFCYRCGILGHSDKVCPDLFELDSDDGVRNWGLDLKPVTTRIGSAATNRWLQDPIPDVVPHQSNFQASSSAGRTHADNSVLNFNNFNNRMLAVDTAINAIKQDVLAAQTALQAKNVTNGAGCQVPMLPSPSTGVSASNLLQGRSMVLGLPAAPFTFAGTNNTQSDNVVFLEDAGSDLKKRKCMLAL