AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016102


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016102.t1 MTR_3g053920 49.180 61 28 1 56 113 153 213 4.63e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCCTAGCAGTTGGCTTT+AGG 0.284244 1.2:-7739777 None:intergenic
TACTGAAGGTTTCCTTGCTT+TGG 0.287642 1.2:+7740002 MS.gene016102:CDS
TAGGACATGATGTTAGTAAT+GGG 0.367803 1.2:+7740187 MS.gene016102:CDS
TGGATGATGGTACGAGGGAG+TGG 0.381604 1.2:+7739746 MS.gene016102:CDS
CACCTCCATGTCAAAGAGTT+GGG 0.392064 1.2:-7739725 None:intergenic
ATTATGTGTCTGCTGCTACT+AGG 0.404274 1.2:+7740145 MS.gene016102:CDS
TTATGTGTCTGCTGCTACTA+GGG 0.404984 1.2:+7740146 MS.gene016102:CDS
CAGCTCTTACATGCGTATAA+AGG 0.405716 1.2:+7739555 None:intergenic
CAAGTTCAAGTATGAAAAGC+TGG 0.410170 1.2:+7739651 MS.gene016102:CDS
GGCGTCACAGTTGTGGGAGC+TGG 0.430451 1.2:-7739916 None:intergenic
CCTTGCTTTGGCTGCTGCAG+AGG 0.433326 1.2:+7740014 MS.gene016102:CDS
GTGAAAGGTGGGTACCTGTT+TGG 0.433398 1.2:-7739841 None:intergenic
GTACTGCTGCAACGAACATC+TGG 0.435242 1.2:+7739803 MS.gene016102:CDS
ACAATTAGTGCTTGGTATGC+CGG 0.456587 1.2:+7739963 MS.gene016102:CDS
GGATGATGGTACGAGGGAGT+GGG 0.460668 1.2:+7739747 MS.gene016102:CDS
GTCTGCTGCTACTAGGGATG+TGG 0.463751 1.2:+7740152 MS.gene016102:CDS
TGATGGCCCTACTGCTGCTC+AGG 0.464425 1.2:+7739876 MS.gene016102:CDS
GTAGCAGCAGACACATAATC+TGG 0.474051 1.2:-7740141 None:intergenic
TTCACCCATGCTACTGCTGA+TGG 0.487663 1.2:+7739859 MS.gene016102:CDS
ATCGCGAGGCGTCACAGTTG+TGG 0.488516 1.2:-7739923 None:intergenic
TACATGATCCGATTCCAAAC+AGG 0.488872 1.2:+7739827 MS.gene016102:CDS
GTACCTGCACAATTCATAAC+CGG 0.490607 1.2:-7739982 None:intergenic
GGTGGGTACCTGTTTGGAAT+CGG 0.494659 1.2:-7739835 None:intergenic
TGTTATCTGTGTGGTCGACT+TGG 0.498671 1.2:+7739682 MS.gene016102:CDS
GATGATGGTACGAGGGAGTG+GGG 0.501074 1.2:+7739748 MS.gene016102:CDS
TCGCGAGGCGTCACAGTTGT+GGG 0.501636 1.2:-7739922 None:intergenic
CTTCCTCGACAATTAGTGCT+TGG 0.504124 1.2:+7739955 MS.gene016102:CDS
CCACCTCCATGTCAAAGAGT+TGG 0.504247 1.2:-7739726 None:intergenic
GGACATGATGTTAGTAATGG+GGG 0.518713 1.2:+7740189 MS.gene016102:CDS
GGAATGGCAGATTCGTATCA+AGG 0.519722 1.2:+7739609 MS.gene016102:CDS
TCTGCTGCTACTAGGGATGT+GGG 0.528479 1.2:+7740153 MS.gene016102:CDS
ATGAATTGTGCAGGTACTGA+AGG 0.534794 1.2:+7739988 MS.gene016102:CDS
GTAGGACATGATGTTAGTAA+TGG 0.541309 1.2:+7740186 MS.gene016102:CDS
TAGGGCCATCAGCAGTAGCA+TGG 0.542359 1.2:-7739864 None:intergenic
AAGCACTAATTGTCGAGGAA+GGG 0.545096 1.2:-7739953 None:intergenic
TGACCTTCCTGAGCAGCAGT+AGG 0.553878 1.2:-7739883 None:intergenic
AGCACTAATTGTCGAGGAAG+GGG 0.556058 1.2:-7739952 None:intergenic
CAGTAGCATGGGTGAAAGGT+GGG 0.562993 1.2:-7739852 None:intergenic
AGGACATGATGTTAGTAATG+GGG 0.567197 1.2:+7740188 MS.gene016102:CDS
GCAGTAGCATGGGTGAAAGG+TGG 0.568538 1.2:-7739853 None:intergenic
GCTTGAACAACTTCAGCAGA+AGG 0.570147 1.2:-7740114 None:intergenic
CAAGCACTAATTGTCGAGGA+AGG 0.571965 1.2:-7739954 None:intergenic
AAAGCCAACTGCTAGGCATG+TGG 0.573907 1.2:+7739780 MS.gene016102:CDS
CCAACTCTTTGACATGGAGG+TGG 0.578927 1.2:+7739726 MS.gene016102:CDS
TCAGCAGTAGCATGGGTGAA+AGG 0.581109 1.2:-7739856 None:intergenic
AGGGCCATCAGCAGTAGCAT+GGG 0.581787 1.2:-7739863 None:intergenic
TTGTCCCCAACTCTTTGACA+TGG 0.585866 1.2:+7739720 MS.gene016102:CDS
ATGCCGGTTATGAATTGTGC+AGG 0.589325 1.2:+7739979 MS.gene016102:CDS
AAGTTCAAGTATGAAAAGCT+GGG 0.591136 1.2:+7739652 MS.gene016102:CDS
GTACCCACATGCCTAGCAGT+TGG 0.591880 1.2:-7739784 None:intergenic
GACCTTCCTGAGCAGCAGTA+GGG 0.591909 1.2:-7739882 None:intergenic
CCTCTGCAGCAGCCAAAGCA+AGG 0.592720 1.2:-7740014 None:intergenic
TGCTGCAGAGGATGACGAGA+AGG 0.595820 1.2:+7740026 MS.gene016102:CDS
AGTTCCTAAAGCCAACTGCT+AGG 0.601231 1.2:+7739773 MS.gene016102:CDS
GGCCCTACTGCTGCTCAGGA+AGG 0.605506 1.2:+7739880 MS.gene016102:CDS
TCCCCAACTCTTTGACATGG+AGG 0.611945 1.2:+7739723 MS.gene016102:CDS
GGGGACAACCTCTGTCTGTG+TGG 0.614390 1.2:-7739705 None:intergenic
ACCTCCATGTCAAAGAGTTG+GGG 0.617434 1.2:-7739724 None:intergenic
GACATGATGTTAGTAATGGG+GGG 0.617782 1.2:+7740190 MS.gene016102:CDS
TTTGACATGGAGGTGGATGA+TGG 0.620819 1.2:+7739733 MS.gene016102:CDS
CTGCTGCTACTAGGGATGTG+GGG 0.622930 1.2:+7740154 MS.gene016102:CDS
TGGCAGATTCGTATCAAGGA+TGG 0.623078 1.2:+7739613 MS.gene016102:CDS
AAGCCAACTGCTAGGCATGT+GGG 0.636851 1.2:+7739781 MS.gene016102:CDS
CGACTTGGCCACACAGACAG+AGG 0.654227 1.2:+7739697 MS.gene016102:CDS
GGAAGGGGCACATCATCGCG+AGG 0.668568 1.2:-7739937 None:intergenic
TGGAGGTGGATGATGGTACG+AGG 0.671698 1.2:+7739740 MS.gene016102:CDS
ATACCAAGCACTAATTGTCG+AGG 0.675161 1.2:-7739958 None:intergenic
GATGTGGGGATTTCTCATGT+AGG 0.680529 1.2:+7740168 MS.gene016102:CDS
GATGACGAGAAGGACATGCA+AGG 0.680965 1.2:+7740036 MS.gene016102:CDS
GGAGGTGGATGATGGTACGA+GGG 0.700384 1.2:+7739741 MS.gene016102:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGTTCAAGTATGAAAAGCT+GGG + chr1.2:7739652-7739671 MS.gene016102:CDS 30.0%
TAGGACATGATGTTAGTAAT+GGG + chr1.2:7740187-7740206 MS.gene016102:CDS 30.0%
AAAAAACGTAAGCGTGCAAT+GGG + chr1.2:7740069-7740088 MS.gene016102:CDS 35.0%
AGGACATGATGTTAGTAATG+GGG + chr1.2:7740188-7740207 MS.gene016102:CDS 35.0%
CAAGTTCAAGTATGAAAAGC+TGG + chr1.2:7739651-7739670 MS.gene016102:CDS 35.0%
GTAGGACATGATGTTAGTAA+TGG + chr1.2:7740186-7740205 MS.gene016102:CDS 35.0%
AAGCACTAATTGTCGAGGAA+GGG - chr1.2:7739956-7739975 None:intergenic 40.0%
ACAATTAGTGCTTGGTATGC+CGG + chr1.2:7739963-7739982 MS.gene016102:CDS 40.0%
ATACCAAGCACTAATTGTCG+AGG - chr1.2:7739961-7739980 None:intergenic 40.0%
ATTATGTGTCTGCTGCTACT+AGG + chr1.2:7740145-7740164 MS.gene016102:CDS 40.0%
GAAAAAACGTAAGCGTGCAA+TGG + chr1.2:7740068-7740087 MS.gene016102:CDS 40.0%
GGACATGATGTTAGTAATGG+GGG + chr1.2:7740189-7740208 MS.gene016102:CDS 40.0%
GTACCTGCACAATTCATAAC+CGG - chr1.2:7739985-7740004 None:intergenic 40.0%
TACATGATCCGATTCCAAAC+AGG + chr1.2:7739827-7739846 MS.gene016102:CDS 40.0%
TACTGAAGGTTTCCTTGCTT+TGG + chr1.2:7740002-7740021 MS.gene016102:CDS 40.0%
TTATGTGTCTGCTGCTACTA+GGG + chr1.2:7740146-7740165 MS.gene016102:CDS 40.0%
! GACATGATGTTAGTAATGGG+GGG + chr1.2:7740190-7740209 MS.gene016102:CDS 40.0%
!! ATGAATTGTGCAGGTACTGA+AGG + chr1.2:7739988-7740007 MS.gene016102:CDS 40.0%
!! GGCACTTTTTGTTATCTGTG+TGG + chr1.2:7739673-7739692 MS.gene016102:CDS 40.0%
!!! ATCTGCCATTCCTTTTTTGG+CGG - chr1.2:7739601-7739620 None:intergenic 40.0%
!!! CGAATCTGCCATTCCTTTTT+TGG - chr1.2:7739604-7739623 None:intergenic 40.0%
ACCTCCATGTCAAAGAGTTG+GGG - chr1.2:7739727-7739746 None:intergenic 45.0%
AGCACTAATTGTCGAGGAAG+GGG - chr1.2:7739955-7739974 None:intergenic 45.0%
AGTTCCTAAAGCCAACTGCT+AGG + chr1.2:7739773-7739792 MS.gene016102:CDS 45.0%
CAAGCACTAATTGTCGAGGA+AGG - chr1.2:7739957-7739976 None:intergenic 45.0%
CACCTCCATGTCAAAGAGTT+GGG - chr1.2:7739728-7739747 None:intergenic 45.0%
CTTCCTCGACAATTAGTGCT+TGG + chr1.2:7739955-7739974 MS.gene016102:CDS 45.0%
GATGTGGGGATTTCTCATGT+AGG + chr1.2:7740168-7740187 MS.gene016102:CDS 45.0%
GCTTGAACAACTTCAGCAGA+AGG - chr1.2:7740117-7740136 None:intergenic 45.0%
GGAATGGCAGATTCGTATCA+AGG + chr1.2:7739609-7739628 MS.gene016102:CDS 45.0%
GTAGCAGCAGACACATAATC+TGG - chr1.2:7740144-7740163 None:intergenic 45.0%
TATCACTCAGCCGCCAAAAA+AGG + chr1.2:7739588-7739607 MS.gene016102:CDS 45.0%
TGGCAGATTCGTATCAAGGA+TGG + chr1.2:7739613-7739632 MS.gene016102:CDS 45.0%
TGTTATCTGTGTGGTCGACT+TGG + chr1.2:7739682-7739701 MS.gene016102:CDS 45.0%
TTGTCCCCAACTCTTTGACA+TGG + chr1.2:7739720-7739739 MS.gene016102:CDS 45.0%
TTTGACATGGAGGTGGATGA+TGG + chr1.2:7739733-7739752 MS.gene016102:CDS 45.0%
! ATGCCGGTTATGAATTGTGC+AGG + chr1.2:7739979-7739998 MS.gene016102:CDS 45.0%
AAAGCCAACTGCTAGGCATG+TGG + chr1.2:7739780-7739799 MS.gene016102:CDS 50.0%
AAGCCAACTGCTAGGCATGT+GGG + chr1.2:7739781-7739800 MS.gene016102:CDS 50.0%
CAGTAGCATGGGTGAAAGGT+GGG - chr1.2:7739855-7739874 None:intergenic 50.0%
CCAACTCTTTGACATGGAGG+TGG + chr1.2:7739726-7739745 MS.gene016102:CDS 50.0%
CCACCTCCATGTCAAAGAGT+TGG - chr1.2:7739729-7739748 None:intergenic 50.0%
CTCAGCCGCCAAAAAAGGAA+TGG + chr1.2:7739593-7739612 MS.gene016102:CDS 50.0%
GATGACGAGAAGGACATGCA+AGG + chr1.2:7740036-7740055 MS.gene016102:CDS 50.0%
GTACTGCTGCAACGAACATC+TGG + chr1.2:7739803-7739822 MS.gene016102:CDS 50.0%
GTGAAAGGTGGGTACCTGTT+TGG - chr1.2:7739844-7739863 None:intergenic 50.0%
TCAGCAGTAGCATGGGTGAA+AGG - chr1.2:7739859-7739878 None:intergenic 50.0%
TCCCCAACTCTTTGACATGG+AGG + chr1.2:7739723-7739742 MS.gene016102:CDS 50.0%
TCTGCTGCTACTAGGGATGT+GGG + chr1.2:7740153-7740172 MS.gene016102:CDS 50.0%
TTCACCCATGCTACTGCTGA+TGG + chr1.2:7739859-7739878 MS.gene016102:CDS 50.0%
! GGTGGGTACCTGTTTGGAAT+CGG - chr1.2:7739838-7739857 None:intergenic 50.0%
!! CATGCCTAGCAGTTGGCTTT+AGG - chr1.2:7739780-7739799 None:intergenic 50.0%
AGGGCCATCAGCAGTAGCAT+GGG - chr1.2:7739866-7739885 None:intergenic 55.0%
CTGCTGCTACTAGGGATGTG+GGG + chr1.2:7740154-7740173 MS.gene016102:CDS 55.0%
GACCTTCCTGAGCAGCAGTA+GGG - chr1.2:7739885-7739904 None:intergenic 55.0%
GCAGTAGCATGGGTGAAAGG+TGG - chr1.2:7739856-7739875 None:intergenic 55.0%
GTACCCACATGCCTAGCAGT+TGG - chr1.2:7739787-7739806 None:intergenic 55.0%
GTCTGCTGCTACTAGGGATG+TGG + chr1.2:7740152-7740171 MS.gene016102:CDS 55.0%
TAGGGCCATCAGCAGTAGCA+TGG - chr1.2:7739867-7739886 None:intergenic 55.0%
TGACCTTCCTGAGCAGCAGT+AGG - chr1.2:7739886-7739905 None:intergenic 55.0%
TGCTGCAGAGGATGACGAGA+AGG + chr1.2:7740026-7740045 MS.gene016102:CDS 55.0%
! GATGATGGTACGAGGGAGTG+GGG + chr1.2:7739748-7739767 MS.gene016102:CDS 55.0%
! GGAGGTGGATGATGGTACGA+GGG + chr1.2:7739741-7739760 MS.gene016102:CDS 55.0%
! GGATGATGGTACGAGGGAGT+GGG + chr1.2:7739747-7739766 MS.gene016102:CDS 55.0%
! TGGAGGTGGATGATGGTACG+AGG + chr1.2:7739740-7739759 MS.gene016102:CDS 55.0%
! TGGATGATGGTACGAGGGAG+TGG + chr1.2:7739746-7739765 MS.gene016102:CDS 55.0%
CCTCTGCAGCAGCCAAAGCA+AGG - chr1.2:7740017-7740036 None:intergenic 60.0%
CCTTGCTTTGGCTGCTGCAG+AGG + chr1.2:7740014-7740033 MS.gene016102:CDS 60.0%
GGGGACAACCTCTGTCTGTG+TGG - chr1.2:7739708-7739727 None:intergenic 60.0%
TGATGGCCCTACTGCTGCTC+AGG + chr1.2:7739876-7739895 MS.gene016102:CDS 60.0%
! ATCGCGAGGCGTCACAGTTG+TGG - chr1.2:7739926-7739945 None:intergenic 60.0%
! CGACTTGGCCACACAGACAG+AGG + chr1.2:7739697-7739716 MS.gene016102:CDS 60.0%
! TCGCGAGGCGTCACAGTTGT+GGG - chr1.2:7739925-7739944 None:intergenic 60.0%
GGCCCTACTGCTGCTCAGGA+AGG + chr1.2:7739880-7739899 MS.gene016102:CDS 65.0%
GGCGTCACAGTTGTGGGAGC+TGG - chr1.2:7739919-7739938 None:intergenic 65.0%
! GGAAGGGGCACATCATCGCG+AGG - chr1.2:7739940-7739959 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 7739565 7740221 7739565 ID=MS.gene016102
chr1.2 mRNA 7739565 7740221 7739565 ID=MS.gene016102.t1;Parent=MS.gene016102
chr1.2 exon 7739565 7740221 7739565 ID=MS.gene016102.t1.exon1;Parent=MS.gene016102.t1
chr1.2 CDS 7739565 7740221 7739565 ID=cds.MS.gene016102.t1;Parent=MS.gene016102.t1
Gene Sequence

>MS.gene016102

ATGCGTATAAAGGTGCGTATTGATATCACTCAGCCGCCAAAAAAGGAATGGCAGATTCGTATCAAGGATGGTAGTTTTGCTACTATCAAGTTCAAGTATGAAAAGCTGGGCACTTTTTGTTATCTGTGTGGTCGACTTGGCCACACAGACAGAGGTTGTCCCCAACTCTTTGACATGGAGGTGGATGATGGTACGAGGGAGTGGGGAGAGTTCCTAAAGCCAACTGCTAGGCATGTGGGTACTGCTGCAACGAACATCTGGCTACATGATCCGATTCCAAACAGGTACCCACCTTTCACCCATGCTACTGCTGATGGCCCTACTGCTGCTCAGGAAGGTCAGTCATCTTATCCAGCTCCCACAACTGTGACGCCTCGCGATGATGTGCCCCTTCCTCGACAATTAGTGCTTGGTATGCCGGTTATGAATTGTGCAGGTACTGAAGGTTTCCTTGCTTTGGCTGCTGCAGAGGATGACGAGAAGGACATGCAAGGAACAGAACTGAAAAAACGTAAGCGTGCAATGGGATCAGATGCTAGTGTTGAGATTCCTTCTGCTGAAGTTGTTCAAGCTGAACCAGATTATGTGTCTGCTGCTACTAGGGATGTGGGGATTTCTCATGTAGGACATGATGTTAGTAATGGGGGGATGATGTAA

Protein sequence

>MS.gene016102.t1

MRIKVRIDITQPPKKEWQIRIKDGSFATIKFKYEKLGTFCYLCGRLGHTDRGCPQLFDMEVDDGTREWGEFLKPTARHVGTAATNIWLHDPIPNRYPPFTHATADGPTAAQEGQSSYPAPTTVTPRDDVPLPRQLVLGMPVMNCAGTEGFLALAAAEDDEKDMQGTELKKRKRAMGSDASVEIPSAEVVQAEPDYVSAATRDVGISHVGHDVSNGGMM