AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42903

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42903.t1 MTR_3g053920 37.615 218 124 4 1 216 1 208 1.05e-37 138
MS.gene42903.t1 MTR_2g061500 50.000 92 46 0 1 92 1 92 4.69e-25 100
MS.gene42903.t1 MTR_8g463110 44.444 99 55 0 335 433 220 318 3.06e-22 99.8
MS.gene42903.t1 MTR_4g020630 46.465 99 53 0 335 433 156 254 5.55e-22 98.6
MS.gene42903.t1 MTR_6g034215 43.434 99 56 0 335 433 224 322 8.82e-21 93.2
MS.gene42903.t1 MTR_1g100687 47.297 74 39 0 335 408 158 231 7.42e-16 77.0
MS.gene42903.t1 MTR_7g063500 41.667 60 35 0 374 433 37 96 7.95e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 107 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCTGCCACTCGTGTTTC+AGG 0.155042 6.4:-42579684 None:intergenic
AGAACCAAGATTTGATTATA+TGG 0.304906 6.4:+42581242 MS.gene42903:CDS
GGATGATGGGTCACGTGGTT+GGG 0.310299 6.4:+42579831 MS.gene42903:CDS
TGGCTCAGGATGATGCTTAT+TGG 0.318888 6.4:+42581478 MS.gene42903:CDS
GTTCAGCTTCAACCAATATA+TGG 0.332486 6.4:+42579887 MS.gene42903:CDS
TGCACATTATTTCCATTCAT+TGG 0.338529 6.4:-42580172 None:intergenic
GTGCTCACGCCGGTTTGATA+TGG 0.347598 6.4:+42579804 MS.gene42903:CDS
TACTGCTGAAACACAAATTC+TGG 0.351179 6.4:+42579232 MS.gene42903:CDS
TTCAACCAATATATGGCTTC+AGG 0.358822 6.4:+42579894 MS.gene42903:CDS
AGGTTTCAATGGCTTGCATA+TGG 0.362801 6.4:+42579963 MS.gene42903:CDS
CATGCACAGGTTGCTGGCTC+AGG 0.369810 6.4:+42581464 MS.gene42903:CDS
GATAAGGGAATGAGTCTCTT+AGG 0.371406 6.4:+42580391 MS.gene42903:CDS
CTTCACTGGACGCCATATAT+AGG 0.373312 6.4:-42579324 None:intergenic
CAAGCGCATGCACAGGTTGC+TGG 0.375452 6.4:+42581458 MS.gene42903:CDS
ATTCTGGACCACTTCCTGAT+TGG 0.396897 6.4:+42579248 MS.gene42903:CDS
TTTACCATATAATCAAATCT+TGG 0.397079 6.4:-42581246 None:intergenic
AGGATCCTGAAGCCATATAT+TGG 0.404648 6.4:-42579899 None:intergenic
TTCAGGATCCTATTCCTTCA+CGG 0.405219 6.4:+42579911 MS.gene42903:CDS
GTCTAATGTAGATGGGGATA+AGG 0.405876 6.4:+42580375 MS.gene42903:CDS
GGTCACGTGGTTGGGGAGAA+TGG 0.408683 6.4:+42579839 MS.gene42903:CDS
AGGAACGCATGGCCTATATA+TGG 0.409883 6.4:+42579312 MS.gene42903:CDS
CGGACAGAGTGAGGCCTTCA+AGG 0.412610 6.4:-42579178 None:intergenic
GTTCCAGCAGCAGGTTTCAA+TGG 0.412660 6.4:+42579952 MS.gene42903:CDS
ACCTCTGACCTTAACCTTGA+AGG 0.415403 6.4:+42579164 MS.gene42903:CDS
TATCTGCCACTCGTGTTTCA+GGG 0.420811 6.4:-42579683 None:intergenic
AGGGTGCAGAGCTGAAGAAT+TGG 0.427081 6.4:+42580293 MS.gene42903:CDS
TCACTTCTTCCACCAATGAA+TGG 0.429030 6.4:+42580160 MS.gene42903:CDS
TGCTATTTGTGTGGCTGCCT+AGG 0.430802 6.4:+42579766 MS.gene42903:CDS
CATCCAGACAGTGATTGAAT+TGG 0.431370 6.4:-42579388 None:intergenic
GAGTGAGGCCTTCAAGGTTA+AGG 0.432136 6.4:-42579172 None:intergenic
GACCAGCAAGTTCCAGCAGC+AGG 0.437530 6.4:+42579943 MS.gene42903:CDS
TCTAATGTAGATGGGGATAA+GGG 0.438404 6.4:+42580376 MS.gene42903:CDS
GAACCCATGCATCTTATTAC+CGG 0.440837 6.4:-42579868 None:intergenic
AACCTGCTGCTGGAACTTGC+TGG 0.443524 6.4:-42579945 None:intergenic
GTTCCAATTCAATCACTGTC+TGG 0.447913 6.4:+42579385 MS.gene42903:CDS
CAGTGAGCACACGTCCAATC+AGG 0.459668 6.4:-42579262 None:intergenic
TGAATTTGATTCCGAGGCAC+AGG 0.459988 6.4:+42580273 MS.gene42903:CDS
TGAAGGATTGACTCTCAATC+TGG 0.463556 6.4:+42579202 MS.gene42903:CDS
AACTGACTCTGGATTGTCTT+CGG 0.465718 6.4:-42580347 None:intergenic
TGCTGAACCCGTGAAGGAAT+AGG 0.469425 6.4:-42579919 None:intergenic
AGGATGATGGGTCACGTGGT+TGG 0.473500 6.4:+42579830 MS.gene42903:CDS
GATGATGGGTCACGTGGTTG+GGG 0.474373 6.4:+42579832 MS.gene42903:CDS
TCAGGATCCTATTCCTTCAC+GGG 0.475085 6.4:+42579912 MS.gene42903:CDS
AAAGCAAGGACAACTTGGTT+AGG 0.477425 6.4:-42580199 None:intergenic
CAAGCAAAGCAAGGACAACT+TGG 0.483223 6.4:-42580204 None:intergenic
TTAGACTCTAAAACTGACTC+TGG 0.498368 6.4:-42580358 None:intergenic
ACGTGTGCTCACTGACAAAC+AGG 0.501707 6.4:+42579271 MS.gene42903:CDS
ACATCTTATCAGTATGTCCT+AGG 0.504777 6.4:-42579783 None:intergenic
GGTTTCAATGGCTTGCATAT+GGG 0.505285 6.4:+42579964 MS.gene42903:CDS
TGAAACACGAGTGGCAGATA+CGG 0.508378 6.4:+42579686 MS.gene42903:CDS
CAGCTCTGCACCCTGTGCCT+CGG 0.510368 6.4:-42580284 None:intergenic
ACTCTGAACTCGACATCGTC+CGG 0.512555 6.4:+42579988 MS.gene42903:CDS
ATTATATGGTAAATGAGTGT+TGG 0.520623 6.4:+42581256 MS.gene42903:CDS
GGCCTCACTCTGTCCGATGA+AGG 0.521273 6.4:+42579185 MS.gene42903:CDS
CATCCTCCTCCATATCAAAC+CGG 0.529616 6.4:-42579813 None:intergenic
TTTGATATGGAGGAGGATGA+TGG 0.529877 6.4:+42579817 MS.gene42903:CDS
ATTTAACAAAGCGCAACTTC+CGG 0.535226 6.4:+42581199 MS.gene42903:intron
GAAGGTTATTAAAGAAGCTA+AGG 0.536894 6.4:+42579415 MS.gene42903:CDS
GCTATGGTAACCTTCTTCAC+TGG 0.537104 6.4:-42579338 None:intergenic
CTCACGCCGGTTTGATATGG+AGG 0.542061 6.4:+42579807 MS.gene42903:CDS
TTGGGGAGAATGGCTGCGAC+CGG 0.550590 6.4:+42579849 MS.gene42903:CDS
TATATGGCGTCCAGTGAAGA+AGG 0.554263 6.4:+42579328 MS.gene42903:CDS
TAAGTTGAAAATTGAAACTG+AGG 0.555967 6.4:+42581362 MS.gene42903:CDS
AGCTGCTGAATTTGATTCCG+AGG 0.557856 6.4:+42580267 MS.gene42903:CDS
AAGCCATTGAAACCTGCTGC+TGG 0.561305 6.4:-42579955 None:intergenic
GCTGTGTCAGATGAGAATAC+TGG 0.565666 6.4:+42580226 MS.gene42903:CDS
GCCTTCAAGGTTAAGGTCAG+AGG 0.568343 6.4:-42579165 None:intergenic
CAGTTCTTATATAAGACTGA+AGG 0.569929 6.4:+42579639 MS.gene42903:intron
GAGGATATCATCTTCTGAAG+TGG 0.570466 6.4:-42581283 None:intergenic
GCGACCGGTAATAAGATGCA+TGG 0.571466 6.4:+42579864 MS.gene42903:CDS
GAAACACGAGTGGCAGATAC+GGG 0.573485 6.4:+42579687 MS.gene42903:CDS
GAAGAAGGTTACCATAGCAC+TGG 0.576236 6.4:+42579343 MS.gene42903:CDS
TGGCAGATACGGGTAAATGA+TGG 0.577259 6.4:+42579697 MS.gene42903:CDS
ACTTCCGGTTTGAAAATGCA+TGG 0.577640 6.4:+42581214 MS.gene42903:CDS
CTTGCACGCAGTAGTCGCAC+TGG 0.583266 6.4:+42581405 MS.gene42903:CDS
ACATTATTTCCATTCATTGG+TGG 0.588082 6.4:-42580169 None:intergenic
TTGATATGGAGGAGGATGAT+GGG 0.589044 6.4:+42579818 MS.gene42903:CDS
GATTGAATTGGAACAAGAAG+CGG 0.594860 6.4:-42579376 None:intergenic
ACAACGTTGTGCTGAGGAGA+TGG 0.595417 6.4:+42581314 MS.gene42903:CDS
ATTGAGAGTCAATCCTTCAT+CGG 0.597117 6.4:-42579198 None:intergenic
CTGATATTGCCTCTCCAAGT+GGG 0.597883 6.4:+42580125 MS.gene42903:CDS
ATCTGCCACTCGTGTTTCAG+GGG 0.598209 6.4:-42579682 None:intergenic
TGGTCTTGCTGAACCCGTGA+AGG 0.599405 6.4:-42579925 None:intergenic
GTATGTGTCAGCCAGTGCTA+TGG 0.600092 6.4:-42579354 None:intergenic
CGACCGGTAATAAGATGCAT+GGG 0.603494 6.4:+42579865 MS.gene42903:CDS
ACGCCGGTTTGATATGGAGG+AGG 0.606005 6.4:+42579810 MS.gene42903:CDS
ATTAAAGAAGCTAAGGATCG+TGG 0.609851 6.4:+42579422 MS.gene42903:CDS
GCACACGTCCAATCAGGAAG+TGG 0.609977 6.4:-42579256 None:intergenic
ATCCTTCATCGGACAGAGTG+AGG 0.611058 6.4:-42579187 None:intergenic
TCACTGTCTGGATGCTACGA+AGG 0.612442 6.4:+42579397 MS.gene42903:CDS
GAATTTGATTCCGAGGCACA+GGG 0.612643 6.4:+42580274 MS.gene42903:CDS
TCAGCACAACGTTGTTGACG+AGG 0.617525 6.4:-42581306 None:intergenic
TTTAGAGTCTAATGTAGATG+GGG 0.622394 6.4:+42580369 MS.gene42903:CDS
TCGTCAACAACGTTGTGCTG+AGG 0.637782 6.4:+42581308 MS.gene42903:CDS
TGTTACACAAGCGCATGCAC+AGG 0.641252 6.4:+42581451 MS.gene42903:CDS
ACTGATATTGCCTCTCCAAG+TGG 0.642808 6.4:+42580124 MS.gene42903:CDS
CACAACGTTGTTGACGAGGG+AGG 0.648230 6.4:-42581302 None:intergenic
CTGACACAGCAAGCAAAGCA+AGG 0.649849 6.4:-42580213 None:intergenic
CTGATAAGATGTGCTCACGC+CGG 0.658592 6.4:+42579794 MS.gene42903:CDS
GTTAAGGTCAGAGGTAGACA+TGG 0.664777 6.4:-42579156 None:intergenic
CAGCACAACGTTGTTGACGA+GGG 0.666833 6.4:-42581305 None:intergenic
GAGGAGGATGATGGGTCACG+TGG 0.677189 6.4:+42579826 MS.gene42903:CDS
GCTTTATGATAACTACAACA+TGG 0.687185 6.4:+42579444 MS.gene42903:CDS
TTGCACCCCTGAAACACGAG+TGG 0.703718 6.4:+42579677 MS.gene42903:CDS
ACGTTGTGCTGAGGAGATGG+TGG 0.716325 6.4:+42581317 MS.gene42903:CDS
GTGCTGAGGAGATGGTGGTG+TGG 0.721305 6.4:+42581322 MS.gene42903:CDS
GTGTCAGATGAGAATACTGG+TGG 0.751380 6.4:+42580229 MS.gene42903:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACCTATATTTTATTATTA+TGG - chr6.4:42580637-42580656 None:intergenic 10.0%
!! TTATTCAACTAATCATATTA+CGG + chr6.4:42580763-42580782 MS.gene42903:intron 15.0%
!!! TCTTTTTCTGTTAATAATAT+TGG + chr6.4:42580683-42580702 MS.gene42903:intron 15.0%
!! GGTCCATAATAATAAAATAT+AGG + chr6.4:42580631-42580650 MS.gene42903:intron 20.0%
!! TAATGAATTTAAACAATCCT+TGG + chr6.4:42580442-42580461 MS.gene42903:intron 20.0%
!! TGATATCATTAATCTCATTA+TGG - chr6.4:42580047-42580066 None:intergenic 20.0%
!! TTAGTAAGCTCAAAAAAATT+TGG + chr6.4:42580091-42580110 MS.gene42903:intron 20.0%
!! TTTACCATATAATCAAATCT+TGG - chr6.4:42581249-42581268 None:intergenic 20.0%
!!! TTTAATGATATTTTGGATGA+CGG + chr6.4:42580898-42580917 MS.gene42903:intron 20.0%
! AAACCATTAATCAACCAATT+AGG - chr6.4:42580949-42580968 None:intergenic 25.0%
! AGAACCAAGATTTGATTATA+TGG + chr6.4:42581242-42581261 MS.gene42903:CDS 25.0%
! ATTATATGGTAAATGAGTGT+TGG + chr6.4:42581256-42581275 MS.gene42903:CDS 25.0%
! CATTTATCTTCTCAATTTGT+AGG + chr6.4:42580853-42580872 MS.gene42903:intron 25.0%
! GTTAATAATATTGGACATAG+TGG + chr6.4:42580692-42580711 MS.gene42903:intron 25.0%
!! TAAGTTGAAAATTGAAACTG+AGG + chr6.4:42581362-42581381 MS.gene42903:CDS 25.0%
!!! TGGGGATTTTAATGATATTT+TGG + chr6.4:42580891-42580910 MS.gene42903:intron 25.0%
!!! TTAATGATATTTTGGATGAC+GGG + chr6.4:42580899-42580918 MS.gene42903:intron 25.0%
!!! TTTTAGAGTCTAATGTAGAT+GGG + chr6.4:42580368-42580387 MS.gene42903:CDS 25.0%
AATAGCAGGACTTAAAGATT+AGG + chr6.4:42580482-42580501 MS.gene42903:intron 30.0%
ACATTATTTCCATTCATTGG+TGG - chr6.4:42580172-42580191 None:intergenic 30.0%
ACTTGTACTATATGGACATT+TGG + chr6.4:42579509-42579528 MS.gene42903:intron 30.0%
AGAAAATGAGTCTGTTAAGT+TGG + chr6.4:42580513-42580532 MS.gene42903:intron 30.0%
CAGTTCTTATATAAGACTGA+AGG + chr6.4:42579639-42579658 MS.gene42903:intron 30.0%
CTTGTACTATATGGACATTT+GGG + chr6.4:42579510-42579529 MS.gene42903:intron 30.0%
GAAGGTTATTAAAGAAGCTA+AGG + chr6.4:42579415-42579434 MS.gene42903:CDS 30.0%
GCTTTATGATAACTACAACA+TGG + chr6.4:42579444-42579463 MS.gene42903:CDS 30.0%
TGCACATTATTTCCATTCAT+TGG - chr6.4:42580175-42580194 None:intergenic 30.0%
TTATGATAACTACAACATGG+TGG + chr6.4:42579447-42579466 MS.gene42903:intron 30.0%
! TTTAGAGTCTAATGTAGATG+GGG + chr6.4:42580369-42580388 MS.gene42903:CDS 30.0%
!! TAATGATATTTTGGATGACG+GGG + chr6.4:42580900-42580919 MS.gene42903:intron 30.0%
!! TGTTTTAAGTGTGAGAAGTT+GGG + chr6.4:42579736-42579755 MS.gene42903:CDS 30.0%
!!! CAATTATTTTAAGGAACGCA+TGG + chr6.4:42579301-42579320 MS.gene42903:CDS 30.0%
!!! GTTTTAGAGTCTAATGTAGA+TGG + chr6.4:42580367-42580386 MS.gene42903:CDS 30.0%
!!! TTGTTTTAAGTGTGAGAAGT+TGG + chr6.4:42579735-42579754 MS.gene42903:CDS 30.0%
AAATTTAGTAGCTCCAGCTT+CGG + chr6.4:42581131-42581150 MS.gene42903:intron 35.0%
AAGCTCTTAAACCAAGTGTA+AGG - chr6.4:42581024-42581043 None:intergenic 35.0%
AATTAACCTAGAGTGGGAAA+CGG + chr6.4:42579562-42579581 MS.gene42903:intron 35.0%
ACATCTTATCAGTATGTCCT+AGG - chr6.4:42579786-42579805 None:intergenic 35.0%
ACGCAAATCATGGTAATTGA+AGG + chr6.4:42580798-42580817 MS.gene42903:intron 35.0%
ATTAAAGAAGCTAAGGATCG+TGG + chr6.4:42579422-42579441 MS.gene42903:CDS 35.0%
ATTGAGAGTCAATCCTTCAT+CGG - chr6.4:42579201-42579220 None:intergenic 35.0%
ATTTAACAAAGCGCAACTTC+CGG + chr6.4:42581199-42581218 MS.gene42903:intron 35.0%
CATGGTAATTGAAGGCTTAT+AGG + chr6.4:42580806-42580825 MS.gene42903:intron 35.0%
CGTCCTAATTGGTTGATTAA+TGG + chr6.4:42580943-42580962 MS.gene42903:intron 35.0%
CTAGGTTAATTCATCCCAAA+TGG - chr6.4:42579553-42579572 None:intergenic 35.0%
GATTGAATTGGAACAAGAAG+CGG - chr6.4:42579379-42579398 None:intergenic 35.0%
GCTGAAAATGATGTAATAGC+AGG + chr6.4:42580468-42580487 MS.gene42903:intron 35.0%
GTTCAGCTTCAACCAATATA+TGG + chr6.4:42579887-42579906 MS.gene42903:CDS 35.0%
TACACTTGGTTTAAGAGCTT+AGG + chr6.4:42581024-42581043 MS.gene42903:intron 35.0%
TACTGCTGAAACACAAATTC+TGG + chr6.4:42579232-42579251 MS.gene42903:CDS 35.0%
TAGCGTGAACTTGTACTATA+TGG + chr6.4:42579501-42579520 MS.gene42903:intron 35.0%
TCTAATGTAGATGGGGATAA+GGG + chr6.4:42580376-42580395 MS.gene42903:CDS 35.0%
TTCAACCAATATATGGCTTC+AGG + chr6.4:42579894-42579913 MS.gene42903:CDS 35.0%
! AAGTTGCGCTTTGTTAAATG+AGG - chr6.4:42581198-42581217 None:intergenic 35.0%
! ACATCATTTTCAGCATACCA+AGG - chr6.4:42580462-42580481 None:intergenic 35.0%
! AGTTGCGCTTTGTTAAATGA+GGG - chr6.4:42581197-42581216 None:intergenic 35.0%
! CTTAACAGACTCATTTTCTG+CGG - chr6.4:42580512-42580531 None:intergenic 35.0%
! GTTTCCATGCATTTTCAAAC+CGG - chr6.4:42581221-42581240 None:intergenic 35.0%
! TAGGACTGTGGTGTATTTTT+GGG + chr6.4:42580872-42580891 MS.gene42903:intron 35.0%
!! AGACAAGTCGTTATTGATTC+AGG + chr6.4:42580970-42580989 MS.gene42903:intron 35.0%
!! GGCTTGGTTCTAATTATTTC+TGG + chr6.4:42581095-42581114 MS.gene42903:intron 35.0%
!! GGTGCGCTTCAATTATTTTA+AGG + chr6.4:42579292-42579311 MS.gene42903:CDS 35.0%
!! GTTTTAAGTGTGAGAAGTTG+GGG + chr6.4:42579737-42579756 MS.gene42903:CDS 35.0%
!! TTAGACTCTAAAACTGACTC+TGG - chr6.4:42580361-42580380 None:intergenic 35.0%
AAAGCAAGGACAACTTGGTT+AGG - chr6.4:42580202-42580221 None:intergenic 40.0%
AACGGAATCGGTAAGTATCT+TGG + chr6.4:42579580-42579599 MS.gene42903:intron 40.0%
AATTCATTACAACATCCCCC+CGG - chr6.4:42580431-42580450 None:intergenic 40.0%
AGACTGTGCTTTGGCAAATT+AGG + chr6.4:42581074-42581093 MS.gene42903:intron 40.0%
AGGATCCTGAAGCCATATAT+TGG - chr6.4:42579902-42579921 None:intergenic 40.0%
AGGTGCATCAACTACCATTT+GGG + chr6.4:42579536-42579555 MS.gene42903:intron 40.0%
ATGTTGCAAAAAATCGCACG+CGG - chr6.4:42580836-42580855 None:intergenic 40.0%
CATCCAGACAGTGATTGAAT+TGG - chr6.4:42579391-42579410 None:intergenic 40.0%
CTTCTCAATTTGTAGGACTG+TGG + chr6.4:42580860-42580879 MS.gene42903:intron 40.0%
GAACCCATGCATCTTATTAC+CGG - chr6.4:42579871-42579890 None:intergenic 40.0%
GAGATACAAGACGCAAATCA+TGG + chr6.4:42580788-42580807 MS.gene42903:intron 40.0%
GATAAGGGAATGAGTCTCTT+AGG + chr6.4:42580391-42580410 MS.gene42903:CDS 40.0%
GGAAAGATTAGACTGTGCTT+TGG + chr6.4:42581065-42581084 MS.gene42903:intron 40.0%
GGGATGAATTAACCTAGAGT+GGG + chr6.4:42579556-42579575 MS.gene42903:intron 40.0%
GTCTAATGTAGATGGGGATA+AGG + chr6.4:42580375-42580394 MS.gene42903:CDS 40.0%
GTTCCAATTCAATCACTGTC+TGG + chr6.4:42579385-42579404 MS.gene42903:CDS 40.0%
GTTGAGCAAAACGTCCTAAT+TGG + chr6.4:42580932-42580951 MS.gene42903:intron 40.0%
TAATCTTTCCTCTACCGCAT+GGG - chr6.4:42581055-42581074 None:intergenic 40.0%
TATGGACCAAAGTCTCACTT+AGG - chr6.4:42580619-42580638 None:intergenic 40.0%
TCACTTAGGAAGAGGAATTC+CGG - chr6.4:42580605-42580624 None:intergenic 40.0%
TCACTTCTTCCACCAATGAA+TGG + chr6.4:42580160-42580179 MS.gene42903:CDS 40.0%
TCCCTCTAGATAAGTCTTAC+CGG - chr6.4:42580010-42580029 None:intergenic 40.0%
TCCGGTAAGACTTATCTAGA+GGG + chr6.4:42580006-42580025 MS.gene42903:intron 40.0%
TGGGATGAATTAACCTAGAG+TGG + chr6.4:42579555-42579574 MS.gene42903:intron 40.0%
TTCAGGATCCTATTCCTTCA+CGG + chr6.4:42579911-42579930 MS.gene42903:CDS 40.0%
TTGAGGGATATCCTTACACT+TGG + chr6.4:42581010-42581029 MS.gene42903:intron 40.0%
TTGATATGGAGGAGGATGAT+GGG + chr6.4:42579818-42579837 MS.gene42903:CDS 40.0%
TTTGATATGGAGGAGGATGA+TGG + chr6.4:42579817-42579836 MS.gene42903:CDS 40.0%
! AACTGACTCTGGATTGTCTT+CGG - chr6.4:42580350-42580369 None:intergenic 40.0%
! ACTCTCGGATGTTCATTTTG+AGG + chr6.4:42580993-42581012 MS.gene42903:intron 40.0%
! ACTTCCGGTTTGAAAATGCA+TGG + chr6.4:42581214-42581233 MS.gene42903:CDS 40.0%
! AGGTTTCAATGGCTTGCATA+TGG + chr6.4:42579963-42579982 MS.gene42903:CDS 40.0%
! AGTTCCTGATCTGAAGTATC+TGG + chr6.4:42580565-42580584 MS.gene42903:intron 40.0%
! CTCTCGGATGTTCATTTTGA+GGG + chr6.4:42580994-42581013 MS.gene42903:intron 40.0%
! GAGGATATCATCTTCTGAAG+TGG - chr6.4:42581286-42581305 None:intergenic 40.0%
! GGTTTCAATGGCTTGCATAT+GGG + chr6.4:42579964-42579983 MS.gene42903:CDS 40.0%
! GTAGGACTGTGGTGTATTTT+TGG + chr6.4:42580871-42580890 MS.gene42903:intron 40.0%
! GTGGTAGTCTTGCTTTATTC+TGG + chr6.4:42580711-42580730 MS.gene42903:intron 40.0%
! TAGGTTTTGCGTTACGTTCT+TGG + chr6.4:42580650-42580669 MS.gene42903:intron 40.0%
! TGAAGGATTGACTCTCAATC+TGG + chr6.4:42579202-42579221 MS.gene42903:CDS 40.0%
!! AGGACTGTGGTGTATTTTTG+GGG + chr6.4:42580873-42580892 MS.gene42903:intron 40.0%
!! CGTTATTGATTCAGGACTCT+CGG + chr6.4:42580978-42580997 MS.gene42903:intron 40.0%
!! TTAGGTGCTGATGTTTGTAC+CGG + chr6.4:42580409-42580428 MS.gene42903:intron 40.0%
!!! GGGACTTTTTGCTATTTGTG+TGG + chr6.4:42579757-42579776 MS.gene42903:CDS 40.0%
AATCTTTCCTCTACCGCATG+GGG - chr6.4:42581054-42581073 None:intergenic 45.0%
ACCTCTGACCTTAACCTTGA+AGG + chr6.4:42579164-42579183 MS.gene42903:CDS 45.0%
ACTGATATTGCCTCTCCAAG+TGG + chr6.4:42580124-42580143 MS.gene42903:CDS 45.0%
AGCTGCTGAATTTGATTCCG+AGG + chr6.4:42580267-42580286 MS.gene42903:CDS 45.0%
AGGAACGCATGGCCTATATA+TGG + chr6.4:42579312-42579331 MS.gene42903:CDS 45.0%
AGTACAAGTTCACGCTAGCT+GGG - chr6.4:42579498-42579517 None:intergenic 45.0%
AGTTGGAACTGTCGAGGTTT+AGG + chr6.4:42580530-42580549 MS.gene42903:intron 45.0%
ATAGGATAGTGATCCGAAGC+TGG - chr6.4:42581147-42581166 None:intergenic 45.0%
ATTCTGGACCACTTCCTGAT+TGG + chr6.4:42579248-42579267 MS.gene42903:CDS 45.0%
CAAGCAAAGCAAGGACAACT+TGG - chr6.4:42580207-42580226 None:intergenic 45.0%
CAGGTGCATCAACTACCATT+TGG + chr6.4:42579535-42579554 MS.gene42903:intron 45.0%
CATCCTCCTCCATATCAAAC+CGG - chr6.4:42579816-42579835 None:intergenic 45.0%
CCAAAGTCTCACTTAGGAAG+AGG - chr6.4:42580613-42580632 None:intergenic 45.0%
CCTCTTCCTAAGTGAGACTT+TGG + chr6.4:42580610-42580629 MS.gene42903:intron 45.0%
CGACCGGTAATAAGATGCAT+GGG + chr6.4:42579865-42579884 MS.gene42903:CDS 45.0%
CTAATCTTTCCTCTACCGCA+TGG - chr6.4:42581056-42581075 None:intergenic 45.0%
CTATATGGACATTTGGGTCC+AGG + chr6.4:42579516-42579535 MS.gene42903:intron 45.0%
CTGATATTGCCTCTCCAAGT+GGG + chr6.4:42580125-42580144 MS.gene42903:CDS 45.0%
CTTCACTGGACGCCATATAT+AGG - chr6.4:42579327-42579346 None:intergenic 45.0%
GAAGAAGGTTACCATAGCAC+TGG + chr6.4:42579343-42579362 MS.gene42903:CDS 45.0%
GAATTTGATTCCGAGGCACA+GGG + chr6.4:42580274-42580293 MS.gene42903:CDS 45.0%
GCTATGGTAACCTTCTTCAC+TGG - chr6.4:42579341-42579360 None:intergenic 45.0%
GCTGTGTCAGATGAGAATAC+TGG + chr6.4:42580226-42580245 MS.gene42903:CDS 45.0%
GGAATTCCGGATTGTAGTGT+CGG - chr6.4:42580592-42580611 None:intergenic 45.0%
GGAGTACGGTTCAACAGAAT+AGG - chr6.4:42581165-42581184 None:intergenic 45.0%
GTCCGGTAAGACTTATCTAG+AGG + chr6.4:42580005-42580024 MS.gene42903:intron 45.0%
GTGCTTTGGCAAATTAGGCT+TGG + chr6.4:42581079-42581098 MS.gene42903:intron 45.0%
GTGTCAGATGAGAATACTGG+TGG + chr6.4:42580229-42580248 MS.gene42903:CDS 45.0%
TAGTACAAGTTCACGCTAGC+TGG - chr6.4:42579499-42579518 None:intergenic 45.0%
TATATGGCGTCCAGTGAAGA+AGG + chr6.4:42579328-42579347 MS.gene42903:CDS 45.0%
TATCTGCCACTCGTGTTTCA+GGG - chr6.4:42579686-42579705 None:intergenic 45.0%
TCAGGATCCTATTCCTTCAC+GGG + chr6.4:42579912-42579931 MS.gene42903:CDS 45.0%
TGAAACACGAGTGGCAGATA+CGG + chr6.4:42579686-42579705 MS.gene42903:CDS 45.0%
TGAATTTGATTCCGAGGCAC+AGG + chr6.4:42580273-42580292 MS.gene42903:CDS 45.0%
TGATATCAAGTCTCGCCTCA+CGG + chr6.4:42580059-42580078 MS.gene42903:intron 45.0%
TGGCAGATACGGGTAAATGA+TGG + chr6.4:42579697-42579716 MS.gene42903:CDS 45.0%
TGGCTCAGGATGATGCTTAT+TGG + chr6.4:42581478-42581497 MS.gene42903:CDS 45.0%
! CAAATGGTAGTTGATGCACC+TGG - chr6.4:42579537-42579556 None:intergenic 45.0%
! GATGAAGCTTTTGTCCCACT+TGG - chr6.4:42580142-42580161 None:intergenic 45.0%
!! CTGTTAAGTTGGAACTGTCG+AGG + chr6.4:42580524-42580543 MS.gene42903:intron 45.0%
!! TAGGTGCTGATGTTTGTACC+GGG + chr6.4:42580410-42580429 MS.gene42903:intron 45.0%
AAGCCATTGAAACCTGCTGC+TGG - chr6.4:42579958-42579977 None:intergenic 50.0%
ACAACGTTGTGCTGAGGAGA+TGG + chr6.4:42581314-42581333 MS.gene42903:CDS 50.0%
ACGTGTGCTCACTGACAAAC+AGG + chr6.4:42579271-42579290 MS.gene42903:CDS 50.0%
ACTCTGAACTCGACATCGTC+CGG + chr6.4:42579988-42580007 MS.gene42903:CDS 50.0%
AGGGTGCAGAGCTGAAGAAT+TGG + chr6.4:42580293-42580312 MS.gene42903:CDS 50.0%
ATCCTTCATCGGACAGAGTG+AGG - chr6.4:42579190-42579209 None:intergenic 50.0%
ATCTGCCACTCGTGTTTCAG+GGG - chr6.4:42579685-42579704 None:intergenic 50.0%
CAGCACAACGTTGTTGACGA+GGG - chr6.4:42581308-42581327 None:intergenic 50.0%
CCTAGAGTGGGAAACGGAAT+CGG + chr6.4:42579568-42579587 MS.gene42903:intron 50.0%
CGGACCAGATACTTCAGATC+AGG - chr6.4:42580572-42580591 None:intergenic 50.0%
CTGACACAGCAAGCAAAGCA+AGG - chr6.4:42580216-42580235 None:intergenic 50.0%
CTGATAAGATGTGCTCACGC+CGG + chr6.4:42579794-42579813 MS.gene42903:CDS 50.0%
GAAACACGAGTGGCAGATAC+GGG + chr6.4:42579687-42579706 MS.gene42903:CDS 50.0%
GAGTGAGGCCTTCAAGGTTA+AGG - chr6.4:42579175-42579194 None:intergenic 50.0%
GCCTTCAAGGTTAAGGTCAG+AGG - chr6.4:42579168-42579187 None:intergenic 50.0%
GCGACCGGTAATAAGATGCA+TGG + chr6.4:42579864-42579883 MS.gene42903:CDS 50.0%
GTATCTGCCACTCGTGTTTC+AGG - chr6.4:42579687-42579706 None:intergenic 50.0%
GTATGTGTCAGCCAGTGCTA+TGG - chr6.4:42579357-42579376 None:intergenic 50.0%
GTTCCAGCAGCAGGTTTCAA+TGG + chr6.4:42579952-42579971 MS.gene42903:CDS 50.0%
TACTAAGCTAGACAGCCGTG+AGG - chr6.4:42580077-42580096 None:intergenic 50.0%
TCACTGTCTGGATGCTACGA+AGG + chr6.4:42579397-42579416 MS.gene42903:CDS 50.0%
TCAGATCAGGAACTGCACTC+AGG - chr6.4:42580559-42580578 None:intergenic 50.0%
TCAGCACAACGTTGTTGACG+AGG - chr6.4:42581309-42581328 None:intergenic 50.0%
TCGTCAACAACGTTGTGCTG+AGG + chr6.4:42581308-42581327 MS.gene42903:CDS 50.0%
TCTGGTCCGACACTACAATC+CGG + chr6.4:42580583-42580602 MS.gene42903:intron 50.0%
TGCTGAACCCGTGAAGGAAT+AGG - chr6.4:42579922-42579941 None:intergenic 50.0%
TGTTACACAAGCGCATGCAC+AGG + chr6.4:42581451-42581470 MS.gene42903:CDS 50.0%
! AGCTTTTGTCCCACTTGGAG+AGG - chr6.4:42580137-42580156 None:intergenic 50.0%
! GTTAAATGAGGGCGCACAAC+AGG - chr6.4:42581186-42581205 None:intergenic 50.0%
! TGCTATTTGTGTGGCTGCCT+AGG + chr6.4:42579766-42579785 MS.gene42903:CDS 50.0%
!! AGGTGCTGATGTTTGTACCG+GGG + chr6.4:42580411-42580430 MS.gene42903:intron 50.0%
!! TTGGATGACGGGGAAAAGCA+TGG + chr6.4:42580910-42580929 MS.gene42903:intron 50.0%
AACCTGCTGCTGGAACTTGC+TGG - chr6.4:42579948-42579967 None:intergenic 55.0%
ACGTTGTGCTGAGGAGATGG+TGG + chr6.4:42581317-42581336 MS.gene42903:CDS 55.0%
CACAACGTTGTTGACGAGGG+AGG - chr6.4:42581305-42581324 None:intergenic 55.0%
CAGTGAGCACACGTCCAATC+AGG - chr6.4:42579265-42579284 None:intergenic 55.0%
CCGATTCCGTTTCCCACTCT+AGG - chr6.4:42579571-42579590 None:intergenic 55.0%
GAGCTTAGGTACACCCCATG+CGG + chr6.4:42581038-42581057 MS.gene42903:intron 55.0%
GCACACGTCCAATCAGGAAG+TGG - chr6.4:42579259-42579278 None:intergenic 55.0%
TTGCACCCCTGAAACACGAG+TGG + chr6.4:42579677-42579696 MS.gene42903:CDS 55.0%
! GTGCTGATGTTTGTACCGGG+GGG + chr6.4:42580413-42580432 MS.gene42903:intron 55.0%
! TGGTCTTGCTGAACCCGTGA+AGG - chr6.4:42579928-42579947 None:intergenic 55.0%
!! ACGCCGGTTTGATATGGAGG+AGG + chr6.4:42579810-42579829 MS.gene42903:CDS 55.0%
!! AGGATGATGGGTCACGTGGT+TGG + chr6.4:42579830-42579849 MS.gene42903:CDS 55.0%
!! CTCACGCCGGTTTGATATGG+AGG + chr6.4:42579807-42579826 MS.gene42903:CDS 55.0%
!! GATGATGGGTCACGTGGTTG+GGG + chr6.4:42579832-42579851 MS.gene42903:CDS 55.0%
!! GGATGATGGGTCACGTGGTT+GGG + chr6.4:42579831-42579850 MS.gene42903:CDS 55.0%
!! GGTGCTGATGTTTGTACCGG+GGG + chr6.4:42580412-42580431 MS.gene42903:intron 55.0%
!! GTGCTCACGCCGGTTTGATA+TGG + chr6.4:42579804-42579823 MS.gene42903:CDS 55.0%
ACGCTAGCTGGGACGACACT+GGG - chr6.4:42579487-42579506 None:intergenic 60.0%
AGGTACACCCCATGCGGTAG+AGG + chr6.4:42581044-42581063 MS.gene42903:intron 60.0%
CAAGCGCATGCACAGGTTGC+TGG + chr6.4:42581458-42581477 MS.gene42903:CDS 60.0%
CGGACAGAGTGAGGCCTTCA+AGG - chr6.4:42579181-42579200 None:intergenic 60.0%
CTTGCACGCAGTAGTCGCAC+TGG + chr6.4:42581405-42581424 MS.gene42903:CDS 60.0%
GACCAGCAAGTTCCAGCAGC+AGG + chr6.4:42579943-42579962 MS.gene42903:CDS 60.0%
GGCCTCACTCTGTCCGATGA+AGG + chr6.4:42579185-42579204 MS.gene42903:CDS 60.0%
GGTCACGTGGTTGGGGAGAA+TGG + chr6.4:42579839-42579858 MS.gene42903:CDS 60.0%
TTGGGGAGAATGGCTGCGAC+CGG + chr6.4:42579849-42579868 MS.gene42903:CDS 60.0%
! GAGGGCGCACAACAGGAGTA+CGG - chr6.4:42581179-42581198 None:intergenic 60.0%
! GTGCTGAGGAGATGGTGGTG+TGG + chr6.4:42581322-42581341 MS.gene42903:CDS 60.0%
!! CATGCACAGGTTGCTGGCTC+AGG + chr6.4:42581464-42581483 MS.gene42903:CDS 60.0%
!! GAGGAGGATGATGGGTCACG+TGG + chr6.4:42579826-42579845 MS.gene42903:CDS 60.0%
CACGCTAGCTGGGACGACAC+TGG - chr6.4:42579488-42579507 None:intergenic 65.0%
CAGCTCTGCACCCTGTGCCT+CGG - chr6.4:42580287-42580306 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 42579158 42581509 42579158 ID=MS.gene42903
chr6.4 mRNA 42579158 42581509 42579158 ID=MS.gene42903.t1;Parent=MS.gene42903
chr6.4 exon 42579158 42579465 42579158 ID=MS.gene42903.t1.exon1;Parent=MS.gene42903.t1
chr6.4 CDS 42579158 42579465 42579158 ID=cds.MS.gene42903.t1;Parent=MS.gene42903.t1
chr6.4 exon 42579642 42580009 42579642 ID=MS.gene42903.t1.exon2;Parent=MS.gene42903.t1
chr6.4 CDS 42579642 42580009 42579642 ID=cds.MS.gene42903.t1;Parent=MS.gene42903.t1
chr6.4 exon 42580095 42580420 42580095 ID=MS.gene42903.t1.exon3;Parent=MS.gene42903.t1
chr6.4 CDS 42580095 42580420 42580095 ID=cds.MS.gene42903.t1;Parent=MS.gene42903.t1
chr6.4 exon 42581210 42581509 42581210 ID=MS.gene42903.t1.exon4;Parent=MS.gene42903.t1
chr6.4 CDS 42581210 42581509 42581210 ID=cds.MS.gene42903.t1;Parent=MS.gene42903.t1
Gene Sequence

>MS.gene42903

ATGTCTACCTCTGACCTTAACCTTGAAGGCCTCACTCTGTCCGATGAAGGATTGACTCTCAATCTGGATGTCGTTACTGCTGAAACACAAATTCTGGACCACTTCCTGATTGGACGTGTGCTCACTGACAAACAGGTGCGCTTCAATTATTTTAAGGAACGCATGGCCTATATATGGCGTCCAGTGAAGAAGGTTACCATAGCACTGGCTGACACATACCGCTTCTTGTTCCAATTCAATCACTGTCTGGATGCTACGAAGGTTATTAAAGAAGCTAAGGATCGTGGCTTTATGATAACTACAACATGGTGGTTGAACATATTGCACCCAGTGTCGTCCCAGCTAGCGTGAACTTGTACTATATGGACATTTGGGTCCAGGTGCATCAACTACCATTTGGGATGAATTAACCTAGAGTGGGAAACGGAATCGGTAAGTATCTTGGACACTTAATTGAATTTCATGCTCGCAATACTATTCACAGTTCTTATATAAGACTGAAGGTTCAGATTGATGTTATTGCACCCCTGAAACACGAGTGGCAGATACGGGTAAATGATGGTTCTTATGCAACAATTTGTTTTAAGTGTGAGAAGTTGGGGACTTTTTGCTATTTGTGTGGCTGCCTAGGACATACTGATAAGATGTGCTCACGCCGGTTTGATATGGAGGAGGATGATGGGTCACGTGGTTGGGGAGAATGGCTGCGACCGGTAATAAGATGCATGGGTTCAGCTTCAACCAATATATGGCTTCAGGATCCTATTCCTTCACGGGTTCAGCAAGACCAGCAAGTTCCAGCAGCAGGTTTCAATGGCTTGCATATGGGAACTCTGAACTCGACATCGTCCGGTAAGACTTATCTAGAGGGACGTATTAGTGTTGTCCATAATGAGATTAATGATATCAAGTCTCGCCTCACGGCTGTCTAGCTTAGTAAGCTCAAAAAAATTTGGCATCTCTTTGACTGATATTGCCTCTCCAAGTGGGACAAAAGCTTCATCACTTCTTCCACCAATGAATGGAAATAATGTGCAGCAACCTAACCAAGTTGTCCTTGCTTTGCTTGCTGTGTCAGATGAGAATACTGGTGGTAATAATTATTTTGCAGCTGCTGAATTTGATTCCGAGGCACAGGGTGCAGAGCTGAAGAATTGGAAATGTGCACAAACTGTTGAAGACAAAAGAGCCGAAGACAATCCAGAGTCAGTTTTAGAGTCTAATGTAGATGGGGATAAGGGAATGAGTCTCTTAGGTGCTGATGTTTGTACCGGGGGGATGTTGTAATGAATTTAAACAATCCTTGGTATGCTGAAAATGATGTAATAGCAGGACTTAAAGATTAGGTGTGCCGCAGAAAATGAGTCTGTTAAGTTGGAACTGTCGAGGTTTAGGTAGCCTGAGTGCAGTTCCTGATCTGAAGTATCTGGTCCGACACTACAATCCGGAATTCCTCTTCCTAAGTGAGACTTTGGTCCATAATAATAAAATATAGGTTTTGCGTTACGTTCTTGGTTATGATTCTTCTTTTTCTGTTAATAATATTGGACATAGTGGTAGTCTTGCTTTATTCTGGCGTGCATCTTTTAATTGTCAAATTATTAATTATTCAACTAATCATATTACGGTAGAGATACAAGACGCAAATCATGGTAATTGAAGGCTTATAGGTGTGCCGCGTGCGATTTTTTGCAACATTTATCTTCTCAATTTGTAGGACTGTGGTGTATTTTTGGGGATTTTAATGATATTTTGGATGACGGGGAAAAGCATGGTTGAGCAAAACGTCCTAATTGGTTGATTAATGGTTTCAGACAAGTCGTTATTGATTCAGGACTCTCGGATGTTCATTTTGAGGGATATCCTTACACTTGGTTTAAGAGCTTAGGTACACCCCATGCGGTAGAGGAAAGATTAGACTGTGCTTTGGCAAATTAGGCTTGGTTCTAATTATTTCTGGAAGCATCATTAGAAAATTTAGTAGCTCCAGCTTCGGATCACTATCCTATTCTGTTGAACCGTACTCCTGTTGTGCGCCCTCATTTAACAAAGCGCAACTTCCGGTTTGAAAATGCATGGAAACTAGAACCAAGATTTGATTATATGGTAAATGAGTGTTGGCAAACCACTTCAGAAGATGATATCCTCCCTCGTCAACAACGTTGTGCTGAGGAGATGGTGGTGTGGAGTAGAAATCATTGTAATAAGTTGAAAATTGAAACTGAGGAATGTCGTCATGAATTGAATCTTGCACGCAGTAGTCGCACTGGTGATGAGAAAAAACAGTTAGCACTGTTACACAAGCGCATGCACAGGTTGCTGGCTCAGGATGATGCTTATTGGAGACAATGA
Protein sequence

>MS.gene42903.t1

MSTSDLNLEGLTLSDEGLTLNLDVVTAETQILDHFLIGRVLTDKQVRFNYFKERMAYIWRPVKKVTIALADTYRFLFQFNHCLDATKVIKEAKDRGFMITTTCSYIRLKVQIDVIAPLKHEWQIRVNDGSYATICFKCEKLGTFCYLCGCLGHTDKMCSRRFDMEEDDGSRGWGEWLRPVIRCMGSASTNIWLQDPIPSRVQQDQQVPAAGFNGLHMGTLNSTSSVSSKKFGISLTDIASPSGTKASSLLPPMNGNNVQQPNQVVLALLAVSDENTGGNNYFAAAEFDSEAQGAELKNWKCAQTVEDKRAEDNPESVLESNVDGDKGMSLLGADRNFRFENAWKLEPRFDYMVNECWQTTSEDDILPRQQRCAEEMVVWSRNHCNKLKIETEECRHELNLARSSRTGDEKKQLALLHKRMHRLLAQDDAYWRQ