AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021555

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021555.t1 MTR_3g053920 35.625 320 113 5 1 279 1 268 2.45e-50 171
MS.gene021555.t1 MTR_2g061500 65.672 67 23 0 1 67 1 67 1.30e-21 90.5
MS.gene021555.t1 MTR_6g034215 66.667 45 15 0 358 402 205 249 3.06e-13 70.5
MS.gene021555.t1 MTR_4g020630 62.791 43 16 0 358 400 137 179 1.19e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 100 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAGATACGACCCAAATTT+TGG 0.140965 1.4:-10132123 MS.gene021555:CDS
TCGTATCTGGTTTGTCATTC+AGG 0.166135 1.4:+10132135 None:intergenic
TCAGGCAGTGTTCCAAAATT+TGG 0.172404 1.4:+10132111 None:intergenic
CAGCTTCTTTATGCGGTTAA+AGG 0.194322 1.4:-10131721 MS.gene021555:CDS
CTGGTTTGTCATTCAGGTTT+AGG 0.230087 1.4:+10132141 None:intergenic
TGGTTTGTCATTCAGGTTTA+GGG 0.248359 1.4:+10132142 None:intergenic
TTTGTTGTTGATGGGGTTAA+TGG 0.250082 1.4:-10130994 MS.gene021555:CDS
CGCCATCTGTTCGCTAAAAT+AGG 0.280085 1.4:+10132052 None:intergenic
TTGTTGTTGATGGGGTTAAT+GGG 0.288052 1.4:-10130993 MS.gene021555:CDS
TGCTATAGGTGTGGCCTTCT+CGG 0.310021 1.4:-10131594 MS.gene021555:CDS
CCGAGAACTGATAATGCTTT+TGG 0.315087 1.4:-10131423 MS.gene021555:CDS
GAGATTAATTCTATCAATAA+TGG 0.366563 1.4:-10131312 MS.gene021555:CDS
TCCAAAATTTGGGTCGTATC+TGG 0.372653 1.4:+10132122 None:intergenic
CAGGCAGTGTTCCAAAATTT+GGG 0.377724 1.4:+10132112 None:intergenic
GGGTAAGGCCTTCAAGGTTA+AGG 0.382482 1.4:+10132183 None:intergenic
CTTAAAGTGAATAGTAATAT+AGG 0.387005 1.4:+10131638 None:intergenic
GCATCAAACATTTGTTCAAA+AGG 0.389983 1.4:+10130168 None:intergenic
CAAACATTTGTTCAAAAGGA+TGG 0.400187 1.4:+10130172 None:intergenic
CCAAAAGCATTATCAGTTCT+CGG 0.406482 1.4:+10131423 None:intergenic
TGCCTTGACCAACCCTTTGT+TGG 0.407125 1.4:+10131796 None:intergenic
GTTGAGCCGGCCACCTAGAT+TGG 0.419283 1.4:+10131347 None:intergenic
TCACTTTAAGTACGAGAAGC+TGG 0.423892 1.4:-10131625 MS.gene021555:CDS
AGTCTGAAGCTGAGGATATC+AGG 0.425922 1.4:-10131095 MS.gene021555:CDS
TCGGCACAACAGATGGTATA+GGG 0.430689 1.4:+10131442 None:intergenic
GTCCCAACACGACGGACAAC+AGG 0.431075 1.4:+10131492 None:intergenic
TAAACTCAGCACAACAGGTT+TGG 0.434699 1.4:+10131170 None:intergenic
CAGCGAGGGTAAGGCCTTCA+AGG 0.435192 1.4:+10132177 None:intergenic
GTTAAGGGCATCAGTGTTCA+TGG 0.440376 1.4:+10132199 None:intergenic
GCAGGTAAACTCAGCACAAC+AGG 0.442789 1.4:+10131165 None:intergenic
CCGCTAACACGCATCTGATC+AGG 0.445336 1.4:+10132093 None:intergenic
CAAGGCATTGGTCAATTCCT+TGG 0.446754 1.4:-10131780 MS.gene021555:CDS
ACTGATGCCCTTAACCTTGA+AGG 0.447545 1.4:-10132191 MS.gene021555:CDS
CTCGGCACAACAGATGGTAT+AGG 0.452589 1.4:+10131441 None:intergenic
AACCTTCAGCAGCGAGGGTA+AGG 0.454552 1.4:+10132168 None:intergenic
GCGAACAGATGGCGAAAGCT+TGG 0.458326 1.4:-10132043 MS.gene021555:CDS
GTCCCATCCGTGAGAGCTGC+AGG 0.459405 1.4:+10131147 None:intergenic
TCCTCAGCTTCAGACTCATT+AGG 0.461220 1.4:+10131102 None:intergenic
CTTTGATTTCATCCAACAAA+GGG 0.464397 1.4:-10131808 MS.gene021555:CDS
GGCGTCAGATTCAAAACATC+AGG 0.464815 1.4:-10129944 MS.gene021555:CDS
CCTGATCAGATGCGTGTTAG+CGG 0.469652 1.4:-10132093 MS.gene021555:CDS
TGTTGTTGATGGGGTTAATG+GGG 0.477365 1.4:-10130992 MS.gene021555:CDS
AGTAATAGCGTTACCACTCT+TGG 0.478318 1.4:+10131260 None:intergenic
GGTAAGGCCTTCAAGGTTAA+GGG 0.479483 1.4:+10132184 None:intergenic
CAGCTCTCACGGATGGGACA+AGG 0.480472 1.4:-10131143 MS.gene021555:CDS
AATGTGAATATTTCCAATCT+AGG 0.481584 1.4:-10131360 MS.gene021555:CDS
AAACATTTGTTCAAAAGGAT+GGG 0.500303 1.4:+10130173 None:intergenic
CGACCTGTTGTCCGTCGTGT+TGG 0.501433 1.4:-10131495 MS.gene021555:CDS
GACCTGTTGTCCGTCGTGTT+GGG 0.501768 1.4:-10131494 MS.gene021555:CDS
CTTTGTTGGATGAAATCAAA+GGG 0.503748 1.4:+10131810 None:intergenic
ATGTTGGCGGAATGTCTTCA+AGG 0.504820 1.4:-10131048 MS.gene021555:CDS
GAATCCAACTCAAATAGATC+AGG 0.508776 1.4:+10131552 None:intergenic
GGCCTTACCCTCGCTGCTGA+AGG 0.514468 1.4:-10132170 MS.gene021555:CDS
ATGTCCTGATCTATTTGAGT+TGG 0.514808 1.4:-10131556 MS.gene021555:CDS
CACTTTAAGTACGAGAAGCT+GGG 0.517396 1.4:-10131624 MS.gene021555:CDS
AGAATCGTAACATGTTTGAC+AGG 0.521786 1.4:+10132017 None:intergenic
CAAAGGGTTGGTCAAGGCAT+TGG 0.526940 1.4:-10131792 MS.gene021555:CDS
AATAGTAATATAGGCACCAT+TGG 0.531830 1.4:+10131647 None:intergenic
GATTTCATCCAACAAAGGGT+TGG 0.535891 1.4:-10131804 MS.gene021555:CDS
TGATGACGGTGTCCGTGAGT+GGG 0.536027 1.4:-10131529 MS.gene021555:CDS
GAGTTTACCTGCAGCTCTCA+CGG 0.539483 1.4:-10131154 MS.gene021555:CDS
GATTGTTCAAAACAACTCGT+TGG 0.541576 1.4:-10131283 MS.gene021555:CDS
CTGATGACGGTGTCCGTGAG+TGG 0.545373 1.4:-10131530 MS.gene021555:CDS
CATTATGCAGAGCGTTGAGC+CGG 0.549435 1.4:+10131334 None:intergenic
AGAAGCTGTGAATTGTGTTA+CGG 0.551893 1.4:+10131736 None:intergenic
CCTTTGATTTCATCCAACAA+AGG 0.558202 1.4:-10131809 MS.gene021555:CDS
TCAGTTCTCGGCACAACAGA+TGG 0.561097 1.4:+10131435 None:intergenic
TTTGAGTTGGATTCTGATGA+CGG 0.563105 1.4:-10131543 MS.gene021555:CDS
AGCTCTCACGGATGGGACAA+GGG 0.563949 1.4:-10131142 MS.gene021555:CDS
ACTTCTAACACTGCTGATGA+TGG 0.565392 1.4:-10131384 MS.gene021555:CDS
GGTTAAACCTTCAGCAGCGA+GGG 0.565778 1.4:+10132163 None:intergenic
AACAACTCGTTGGCCAAGAG+TGG 0.567935 1.4:-10131273 MS.gene021555:CDS
AGCAGAAGTACTCGCAGATG+AGG 0.568507 1.4:+10131209 None:intergenic
ATATTTCCAATCTAGGTGGC+CGG 0.569698 1.4:-10131353 MS.gene021555:CDS
ATCCAACAAAGGGTTGGTCA+AGG 0.576913 1.4:-10131798 MS.gene021555:CDS
GAAGCGGAAGCTAATGTTGG+CGG 0.577259 1.4:-10131061 MS.gene021555:CDS
TTACCTGCAGCTCTCACGGA+TGG 0.579490 1.4:-10131150 MS.gene021555:CDS
CCTTTGTTGGATGAAATCAA+AGG 0.584076 1.4:+10131809 None:intergenic
AGGAGCTTGTTACTGATGCA+TGG 0.585252 1.4:-10130079 MS.gene021555:CDS
CGTACAATTTCATTTCGCCA+AGG 0.585667 1.4:+10131763 None:intergenic
GTAACAAGCTCCTTGAAACC+TGG 0.587189 1.4:+10130089 None:intergenic
CGTCCTCATCTTCAAAAGCG+TGG 0.598069 1.4:-10130143 MS.gene021555:CDS
TCCTAATGAGTCTGAAGCTG+AGG 0.598120 1.4:-10131103 MS.gene021555:CDS
GAAGAAGCGGAAGCTAATGT+TGG 0.598173 1.4:-10131064 MS.gene021555:CDS
CAATTCACAGCTTCTTTATG+CGG 0.598935 1.4:-10131728 MS.gene021555:CDS
GAAGTACTCGCAGATGAGGA+AGG 0.599387 1.4:+10131213 None:intergenic
TACCTGCAGCTCTCACGGAT+GGG 0.599974 1.4:-10131149 MS.gene021555:CDS
CCTTCTCGGTCATACTGACA+AGG 0.620615 1.4:-10131580 MS.gene021555:CDS
GGACTGCTGCCACTAACAGA+TGG 0.632438 1.4:-10131473 MS.gene021555:CDS
TCAGGTCAGAAATGAAGAAG+CGG 0.634926 1.4:-10131077 MS.gene021555:CDS
GATGACGGTGTCCGTGAGTG+GGG 0.638116 1.4:-10131528 MS.gene021555:CDS
AAACATGTTACGATTCTGCA+AGG 0.644586 1.4:-10132011 MS.gene021555:intron
GGGTTAAACCTTCAGCAGCG+AGG 0.644636 1.4:+10132162 None:intergenic
TGAAATCAAAGGGTAAACCA+TGG 0.647951 1.4:+10131820 None:intergenic
CCTTGTCAGTATGACCGAGA+AGG 0.653416 1.4:+10131580 None:intergenic
GCAACTCTACACCCCACTCA+CGG 0.653515 1.4:+10131517 None:intergenic
GTGAATATTTCCAATCTAGG+TGG 0.656308 1.4:-10131357 MS.gene021555:CDS
GTTAATGGGGATACTGAACT+AGG 0.658998 1.4:-10130979 MS.gene021555:intron
TGGCAGGTACGAGCAACCAA+TGG 0.664005 1.4:-10131663 MS.gene021555:CDS
TGGCAGCAGTCCCAACACGA+CGG 0.725334 1.4:+10131484 None:intergenic
TTGGCGGAATGTCTTCAAGG+TGG 0.782723 1.4:-10131045 MS.gene021555:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAGTGAATAGTAATAT+AGG + chr1.4:10130498-10130517 None:intergenic 20.0%
!! GAGATTAATTCTATCAATAA+TGG - chr1.4:10130821-10130840 MS.gene021555:intron 20.0%
!!! TCTAAACCATATTGATTTTT+GGG - chr1.4:10130283-10130302 MS.gene021555:intron 20.0%
! AAACATTTGTTCAAAAGGAT+GGG + chr1.4:10131963-10131982 None:intergenic 25.0%
! AATGTGAATATTTCCAATCT+AGG - chr1.4:10130773-10130792 MS.gene021555:intron 25.0%
! CAAAAATCAATATGGTTTAG+AGG + chr1.4:10130284-10130303 None:intergenic 25.0%
! GAGATTAAACCAATGATTAT+TGG + chr1.4:10131889-10131908 None:intergenic 25.0%
!! TTTAAATCTGGAATTGCATT+CGG + chr1.4:10131327-10131346 None:intergenic 25.0%
!!! ACATCATTACTTTGAAACAA+AGG + chr1.4:10131229-10131248 None:intergenic 25.0%
!!! AGTTTTCTTTTGTTGTTGAT+GGG - chr1.4:10131131-10131150 MS.gene021555:CDS 25.0%
!!! CTCTAAACCATATTGATTTT+TGG - chr1.4:10130282-10130301 MS.gene021555:intron 25.0%
AAACCAATGATTATTGGCAA+GGG + chr1.4:10131883-10131902 None:intergenic 30.0%
AAACCATTTATCAACCAGTT+AGG + chr1.4:10131747-10131766 None:intergenic 30.0%
AATAGTAATATAGGCACCAT+TGG + chr1.4:10130489-10130508 None:intergenic 30.0%
ACTGATTAGATAATTGACGA+AGG + chr1.4:10131647-10131666 None:intergenic 30.0%
AGGCGACTTTAATGATATTA+TGG - chr1.4:10131689-10131708 MS.gene021555:CDS 30.0%
AGGTTATAATGTTAGTGAGA+GGG - chr1.4:10131174-10131193 MS.gene021555:CDS 30.0%
CAAACATTTGTTCAAAAGGA+TGG + chr1.4:10131964-10131983 None:intergenic 30.0%
CAATTATCTAATCAGTTTGC+AGG - chr1.4:10131651-10131670 MS.gene021555:CDS 30.0%
CTTTGTTGGATGAAATCAAA+GGG + chr1.4:10130326-10130345 None:intergenic 30.0%
GAAGATTTACGTTATTTGCT+TGG - chr1.4:10131417-10131436 MS.gene021555:CDS 30.0%
GCATCAAACATTTGTTCAAA+AGG + chr1.4:10131968-10131987 None:intergenic 30.0%
GTTTCAATTCCATCACAAAA+TGG - chr1.4:10130163-10130182 MS.gene021555:CDS 30.0%
TAAACCAATGATTATTGGCA+AGG + chr1.4:10131884-10131903 None:intergenic 30.0%
TAGGTTATAATGTTAGTGAG+AGG - chr1.4:10131173-10131192 MS.gene021555:CDS 30.0%
TATGGATGCTAGTGAAAAAA+GGG - chr1.4:10131707-10131726 MS.gene021555:CDS 30.0%
TTATGGATGCTAGTGAAAAA+AGG - chr1.4:10131706-10131725 MS.gene021555:CDS 30.0%
TTCACTTGGTTCAAAAGTTT+GGG - chr1.4:10131822-10131841 MS.gene021555:CDS 30.0%
TTGGTTTAATCTCTTTCCTA+CGG - chr1.4:10131896-10131915 MS.gene021555:intron 30.0%
TTTCACTTGGTTCAAAAGTT+TGG - chr1.4:10131821-10131840 MS.gene021555:CDS 30.0%
! AAACTTTTGAACCAAGTGAA+AGG + chr1.4:10131822-10131841 None:intergenic 30.0%
! ATTTTCGGTAGTAGCTAAAT+TGG - chr1.4:10132091-10132110 MS.gene021555:CDS 30.0%
! CAAAGTAATGATGTATTGAC+AGG - chr1.4:10131235-10131254 MS.gene021555:CDS 30.0%
! CAGATTTAAAGTACCTAGTT+CGG - chr1.4:10131337-10131356 MS.gene021555:CDS 30.0%
! CTTTGATTTCATCCAACAAA+GGG - chr1.4:10130325-10130344 MS.gene021555:intron 30.0%
! TTAAATCTGGAATTGCATTC+GGG + chr1.4:10131326-10131345 None:intergenic 30.0%
!! TTTTGTGATGGAATTGAAAC+AGG + chr1.4:10130163-10130182 None:intergenic 30.0%
!!! GAGTTTTCTTTTGTTGTTGA+TGG - chr1.4:10131130-10131149 MS.gene021555:CDS 30.0%
!!! GTTTTCTTTTGTTGTTGATG+GGG - chr1.4:10131132-10131151 MS.gene021555:CDS 30.0%
AAACATGTTACGATTCTGCA+AGG - chr1.4:10130122-10130141 MS.gene021555:CDS 35.0%
AGAAGCTGTGAATTGTGTTA+CGG + chr1.4:10130400-10130419 None:intergenic 35.0%
AGAATCGTAACATGTTTGAC+AGG + chr1.4:10130119-10130138 None:intergenic 35.0%
AGCGATTATTGATACAGGTT+TGG - chr1.4:10131773-10131792 MS.gene021555:CDS 35.0%
ATGGATGCTAGTGAAAAAAG+GGG - chr1.4:10131708-10131727 MS.gene021555:CDS 35.0%
ATGTCCTGATCTATTTGAGT+TGG - chr1.4:10130577-10130596 MS.gene021555:intron 35.0%
CAATTCACAGCTTCTTTATG+CGG - chr1.4:10130405-10130424 MS.gene021555:intron 35.0%
CCTTTGTTGGATGAAATCAA+AGG + chr1.4:10130327-10130346 None:intergenic 35.0%
CGAACTAGGTACTTTAAATC+TGG + chr1.4:10131339-10131358 None:intergenic 35.0%
CTGTATCAATAATCGCTTGT+CGG + chr1.4:10131770-10131789 None:intergenic 35.0%
CTTGCACCCAAAAATCAATA+TGG + chr1.4:10130292-10130311 None:intergenic 35.0%
GAAAAAATCTTCGACCTAAC+TGG - chr1.4:10131730-10131749 MS.gene021555:CDS 35.0%
GAATCCAACTCAAATAGATC+AGG + chr1.4:10130584-10130603 None:intergenic 35.0%
GATAAATGAGTCTGATAGGA+TGG - chr1.4:10131280-10131299 MS.gene021555:CDS 35.0%
GATGTTAATGATGTTGTCCA+TGG - chr1.4:10131555-10131574 MS.gene021555:CDS 35.0%
GATTATTGATACAGGTTTGG+TGG - chr1.4:10131776-10131795 MS.gene021555:CDS 35.0%
GATTGTTCAAAACAACTCGT+TGG - chr1.4:10130850-10130869 MS.gene021555:intron 35.0%
GGTTATAATGTTAGTGAGAG+GGG - chr1.4:10131175-10131194 MS.gene021555:CDS 35.0%
GTGAATATTTCCAATCTAGG+TGG - chr1.4:10130776-10130795 MS.gene021555:intron 35.0%
TGAAAACTATCATACACCCA+TGG + chr1.4:10130215-10130234 None:intergenic 35.0%
TGAAATCAAAGGGTAAACCA+TGG + chr1.4:10130316-10130335 None:intergenic 35.0%
! AAGCATTGCAATAAGCTGAA+GGG - chr1.4:10132149-10132168 MS.gene021555:CDS 35.0%
! CCTTTGATTTCATCCAACAA+AGG - chr1.4:10130324-10130343 MS.gene021555:intron 35.0%
! GCACACACAATTTGCATTTT+CGG - chr1.4:10132076-10132095 MS.gene021555:CDS 35.0%
! TGGTTTGTCATTCAGGTTTA+GGG + chr1.4:10129994-10130013 None:intergenic 35.0%
! TTGTTGTTGATGGGGTTAAT+GGG - chr1.4:10131140-10131159 MS.gene021555:CDS 35.0%
!! AGAACTGATAATGCTTTTGG+AGG - chr1.4:10130713-10130732 MS.gene021555:intron 35.0%
!! CCAAAAGCATTATCAGTTCT+CGG + chr1.4:10130713-10130732 None:intergenic 35.0%
!! GTATTTTGAACCAGGTTTCA+AGG - chr1.4:10132034-10132053 MS.gene021555:CDS 35.0%
!! TTTGAGTTGGATTCTGATGA+CGG - chr1.4:10130590-10130609 MS.gene021555:intron 35.0%
!! TTTGTTGTTGATGGGGTTAA+TGG - chr1.4:10131139-10131158 MS.gene021555:CDS 35.0%
ACTTCTAACACTGCTGATGA+TGG - chr1.4:10130749-10130768 MS.gene021555:intron 40.0%
AGTAATAGCGTTACCACTCT+TGG + chr1.4:10130876-10130895 None:intergenic 40.0%
ATATTTCCAATCTAGGTGGC+CGG - chr1.4:10130780-10130799 MS.gene021555:intron 40.0%
ATGATGTTGTCCATGGTATG+TGG - chr1.4:10131562-10131581 MS.gene021555:CDS 40.0%
ATGGGATAGTGATCTGAAGA+TGG + chr1.4:10131945-10131964 None:intergenic 40.0%
CACTTTAAGTACGAGAAGCT+GGG - chr1.4:10130509-10130528 MS.gene021555:intron 40.0%
CAGCTTCTTTATGCGGTTAA+AGG - chr1.4:10130412-10130431 MS.gene021555:intron 40.0%
CAGGCAGTGTTCCAAAATTT+GGG + chr1.4:10130024-10130043 None:intergenic 40.0%
CCTATCAGACTCATTTATCC+CGG + chr1.4:10131279-10131298 None:intergenic 40.0%
CGACAAGCGATTATTGATAC+AGG - chr1.4:10131768-10131787 MS.gene021555:CDS 40.0%
CGTACAATTTCATTTCGCCA+AGG + chr1.4:10130373-10130392 None:intergenic 40.0%
CTCGTCCAATGAAAAAACAG+TGG - chr1.4:10130450-10130469 MS.gene021555:intron 40.0%
GATTTCATCCAACAAAGGGT+TGG - chr1.4:10130329-10130348 MS.gene021555:intron 40.0%
GTTAATGGGGATACTGAACT+AGG - chr1.4:10131154-10131173 MS.gene021555:CDS 40.0%
TAAACTCAGCACAACAGGTT+TGG + chr1.4:10130966-10130985 None:intergenic 40.0%
TCACTTTAAGTACGAGAAGC+TGG - chr1.4:10130508-10130527 MS.gene021555:intron 40.0%
TCAGGCAGTGTTCCAAAATT+TGG + chr1.4:10130025-10130044 None:intergenic 40.0%
TCAGGTCAGAAATGAAGAAG+CGG - chr1.4:10131056-10131075 MS.gene021555:CDS 40.0%
TCCAAAATTTGGGTCGTATC+TGG + chr1.4:10130014-10130033 None:intergenic 40.0%
TCCAATGAAAAAACAGTGGC+AGG - chr1.4:10130454-10130473 MS.gene021555:intron 40.0%
TCTGATAGGATGGAATTGCA+GGG - chr1.4:10131290-10131309 MS.gene021555:CDS 40.0%
TGGAAGGATATCCTTTCACT+TGG - chr1.4:10131808-10131827 MS.gene021555:CDS 40.0%
TGGGATAGTGATCTGAAGAT+GGG + chr1.4:10131944-10131963 None:intergenic 40.0%
! ACCAGATACGACCCAAATTT+TGG - chr1.4:10130010-10130029 MS.gene021555:intron 40.0%
! CTGGTTTGTCATTCAGGTTT+AGG + chr1.4:10129995-10130014 None:intergenic 40.0%
! GTTTTCGATACGAAAATGCG+TGG - chr1.4:10132012-10132031 MS.gene021555:CDS 40.0%
! GTTTTGCAACCATCTGTTAG+TGG + chr1.4:10130672-10130691 None:intergenic 40.0%
! TCGTATCTGGTTTGTCATTC+AGG + chr1.4:10130001-10130020 None:intergenic 40.0%
! TGCTTTTCTGTTGATCGTAG+TGG - chr1.4:10131450-10131469 MS.gene021555:CDS 40.0%
! TGTTGTTGATGGGGTTAATG+GGG - chr1.4:10131141-10131160 MS.gene021555:CDS 40.0%
!! AAACCACGCTTTTGAAGATG+AGG + chr1.4:10131996-10132015 None:intergenic 40.0%
!! AATGCGTGGTATTTTGAACC+AGG - chr1.4:10132026-10132045 MS.gene021555:CDS 40.0%
!! AGCTGGGAGTTTTATGCTAT+AGG - chr1.4:10130525-10130544 MS.gene021555:intron 40.0%
!! CCGAGAACTGATAATGCTTT+TGG - chr1.4:10130710-10130729 MS.gene021555:intron 40.0%
!! CGACCTAACTGGTTGATAAA+TGG - chr1.4:10131741-10131760 MS.gene021555:CDS 40.0%
!! GAAGCATTGCAATAAGCTGA+AGG - chr1.4:10132148-10132167 MS.gene021555:CDS 40.0%
!! GGAGTTTTATGCTATAGGTG+TGG - chr1.4:10130530-10130549 MS.gene021555:intron 40.0%
!!! ACCTGCCACTGTTTTTTCAT+TGG + chr1.4:10130458-10130477 None:intergenic 40.0%
!!! TGCCTATTTTAGCGAACAGA+TGG - chr1.4:10130079-10130098 MS.gene021555:CDS 40.0%
AAGTCGCCTAGTATACACCA+AGG + chr1.4:10131678-10131697 None:intergenic 45.0%
AAGTGCGCCAAAAAAGAGCA+AGG + chr1.4:10131488-10131507 None:intergenic 45.0%
ACTGATGCCCTTAACCTTGA+AGG - chr1.4:10129942-10129961 MS.gene021555:CDS 45.0%
ATGTTGGCGGAATGTCTTCA+AGG - chr1.4:10131085-10131104 MS.gene021555:CDS 45.0%
CCGGGATAAATGAGTCTGAT+AGG - chr1.4:10131276-10131295 MS.gene021555:CDS 45.0%
CCTAGTTCGGCACTTCAATT+CGG - chr1.4:10131350-10131369 MS.gene021555:CDS 45.0%
CGCCATCTGTTCGCTAAAAT+AGG + chr1.4:10130084-10130103 None:intergenic 45.0%
CTAATCAGTTTGCAGGTCCT+TGG - chr1.4:10131658-10131677 MS.gene021555:CDS 45.0%
CTGACATGGTTATGTGGAGT+AGG - chr1.4:10132126-10132145 MS.gene021555:CDS 45.0%
CTGATAGGATGGAATTGCAG+GGG - chr1.4:10131291-10131310 MS.gene021555:CDS 45.0%
GAAGAAGCGGAAGCTAATGT+TGG - chr1.4:10131069-10131088 MS.gene021555:CDS 45.0%
GAGCCCTTGCCAATAATCAT+TGG - chr1.4:10131877-10131896 MS.gene021555:intron 45.0%
GGCGTCAGATTCAAAACATC+AGG - chr1.4:10132189-10132208 MS.gene021555:CDS 45.0%
GGGATAGTGATCTGAAGATG+GGG + chr1.4:10131943-10131962 None:intergenic 45.0%
GGTAAGGCCTTCAAGGTTAA+GGG + chr1.4:10129952-10129971 None:intergenic 45.0%
GTAACAAGCTCCTTGAAACC+TGG + chr1.4:10132047-10132066 None:intergenic 45.0%
GTCTGATAGGATGGAATTGC+AGG - chr1.4:10131289-10131308 MS.gene021555:CDS 45.0%
GTGAAAGGATATCCTTCCAC+CGG + chr1.4:10131807-10131826 None:intergenic 45.0%
GTGTCTCGCTTAAGAACAAG+AGG + chr1.4:10131377-10131396 None:intergenic 45.0%
TCCTCAGCTTCAGACTCATT+AGG + chr1.4:10131034-10131053 None:intergenic 45.0%
TCGGCACAACAGATGGTATA+GGG + chr1.4:10130694-10130713 None:intergenic 45.0%
TCTGTTGATCGTAGTGGTAG+AGG - chr1.4:10131456-10131475 MS.gene021555:CDS 45.0%
TGTTACGATTCTGCAAGGTG+AGG - chr1.4:10130127-10130146 MS.gene021555:CDS 45.0%
! AGGGTGATATTGAAGAGTGC+AGG - chr1.4:10132168-10132187 MS.gene021555:CDS 45.0%
! AGTCTGAAGCTGAGGATATC+AGG - chr1.4:10131038-10131057 MS.gene021555:CDS 45.0%
! ATTGACAGGCTCTGATGATC+AGG - chr1.4:10131249-10131268 MS.gene021555:CDS 45.0%
! GTTGTTGATAGAATTGCCCC+TGG - chr1.4:10130239-10130258 MS.gene021555:intron 45.0%
! TCCTAATGAGTCTGAAGCTG+AGG - chr1.4:10131030-10131049 MS.gene021555:CDS 45.0%
! TGGCAGCATCCATTTTGTGA+TGG + chr1.4:10130175-10130194 None:intergenic 45.0%
! TTCAGGGTACCTATAGTAGC+CGG + chr1.4:10131595-10131614 None:intergenic 45.0%
!! AGGAGCTTGTTACTGATGCA+TGG - chr1.4:10132054-10132073 MS.gene021555:CDS 45.0%
!! ATCCAACAAAGGGTTGGTCA+AGG - chr1.4:10130335-10130354 MS.gene021555:intron 45.0%
!! CAAGGCATTGGTCAATTCCT+TGG - chr1.4:10130353-10130372 MS.gene021555:intron 45.0%
!! GCTTTTGGAGGAACACATGT+TGG - chr1.4:10130725-10130744 MS.gene021555:intron 45.0%
!! TACAGGTTTGGTGGATGTTC+CGG - chr1.4:10131785-10131804 MS.gene021555:CDS 45.0%
!!! GATTTTTGGGTGCAAGTCCA+TGG - chr1.4:10130296-10130315 MS.gene021555:intron 45.0%
AACAACTCGTTGGCCAAGAG+TGG - chr1.4:10130860-10130879 MS.gene021555:intron 50.0%
AAGTGCTCGATGAAGGTCCA+TGG - chr1.4:10130195-10130214 MS.gene021555:intron 50.0%
AAGTTTGGGAACACCACGTG+CGG - chr1.4:10131836-10131855 MS.gene021555:intron 50.0%
ACCCTGAAGGAAGACGTAGA+AGG - chr1.4:10131607-10131626 MS.gene021555:CDS 50.0%
AGATTCTCCAGAGTAGCCGT+AGG + chr1.4:10131915-10131934 None:intergenic 50.0%
AGTGCTCGATGAAGGTCCAT+GGG - chr1.4:10130196-10130215 MS.gene021555:intron 50.0%
CATGGTATGTGGAGACTCAC+CGG - chr1.4:10131573-10131592 MS.gene021555:CDS 50.0%
CCCTGAAGGAAGACGTAGAA+GGG - chr1.4:10131608-10131627 MS.gene021555:CDS 50.0%
CCCTTCTACGTCTTCCTTCA+GGG + chr1.4:10131611-10131630 None:intergenic 50.0%
CCTGATCAGATGCGTGTTAG+CGG - chr1.4:10130040-10130059 MS.gene021555:intron 50.0%
CCTTCTCGGTCATACTGACA+AGG - chr1.4:10130553-10130572 MS.gene021555:intron 50.0%
CCTTGTCAGTATGACCGAGA+AGG + chr1.4:10130556-10130575 None:intergenic 50.0%
CGTCCTCATCTTCAAAAGCG+TGG - chr1.4:10131990-10132009 MS.gene021555:intron 50.0%
CTCGGCACAACAGATGGTAT+AGG + chr1.4:10130695-10130714 None:intergenic 50.0%
GAAGCGGAAGCTAATGTTGG+CGG - chr1.4:10131072-10131091 MS.gene021555:CDS 50.0%
GAGTTTACCTGCAGCTCTCA+CGG - chr1.4:10130979-10130998 MS.gene021555:intron 50.0%
GCAGGTAAACTCAGCACAAC+AGG + chr1.4:10130971-10130990 None:intergenic 50.0%
GGATGGAATTGCAGGGGTTT+AGG - chr1.4:10131297-10131316 MS.gene021555:CDS 50.0%
GGCTACTATAGGTACCCTGA+AGG - chr1.4:10131594-10131613 MS.gene021555:CDS 50.0%
GGGTAAGGCCTTCAAGGTTA+AGG + chr1.4:10129953-10129972 None:intergenic 50.0%
GGTTAAACCTTCAGCAGCGA+GGG + chr1.4:10129973-10129992 None:intergenic 50.0%
GTTGATCGTAGTGGTAGAGG+AGG - chr1.4:10131459-10131478 MS.gene021555:CDS 50.0%
TCAGACTCATTTATCCCGGC+AGG + chr1.4:10131275-10131294 None:intergenic 50.0%
TCAGTTCTCGGCACAACAGA+TGG + chr1.4:10130701-10130720 None:intergenic 50.0%
TCTCTTTCCTACGGCTACTC+TGG - chr1.4:10131905-10131924 MS.gene021555:intron 50.0%
TGCCTTGACCAACCCTTTGT+TGG + chr1.4:10130340-10130359 None:intergenic 50.0%
TGCTATAGGTGTGGCCTTCT+CGG - chr1.4:10130539-10130558 MS.gene021555:intron 50.0%
TGGACCTTCATCGAGCACTT+TGG + chr1.4:10130195-10130214 None:intergenic 50.0%
TTGGCGGAATGTCTTCAAGG+TGG - chr1.4:10131088-10131107 MS.gene021555:CDS 50.0%
! GAAGTACTCGCAGATGAGGA+AGG + chr1.4:10130923-10130942 None:intergenic 50.0%
! GCAGGTCCTTGGTGTATACT+AGG - chr1.4:10131669-10131688 MS.gene021555:CDS 50.0%
!! AGCAGAAGTACTCGCAGATG+AGG + chr1.4:10130927-10130946 None:intergenic 50.0%
!! CAAAGGGTTGGTCAAGGCAT+TGG - chr1.4:10130341-10130360 MS.gene021555:intron 50.0%
!! CATTATGCAGAGCGTTGAGC+CGG + chr1.4:10130802-10130821 None:intergenic 50.0%
!! CCGAATTGAAGTGCCGAACT+AGG + chr1.4:10131353-10131372 None:intergenic 50.0%
!!! GAGGTGGCCTTGCTCTTTTT+TGG - chr1.4:10131478-10131497 MS.gene021555:CDS 50.0%
AACACGTTGCACAGCACCAG+GGG + chr1.4:10130258-10130277 None:intergenic 55.0%
AACCTTCAGCAGCGAGGGTA+AGG + chr1.4:10129968-10129987 None:intergenic 55.0%
AAGACGTAGAAGGGCTGCGT+GGG - chr1.4:10131617-10131636 MS.gene021555:CDS 55.0%
ACAACACGTTGCACAGCACC+AGG + chr1.4:10130260-10130279 None:intergenic 55.0%
AGACGTTCTTCAACCGCACG+TGG + chr1.4:10131852-10131871 None:intergenic 55.0%
AGCTCTCACGGATGGGACAA+GGG - chr1.4:10130991-10131010 MS.gene021555:CDS 55.0%
CAACACGTTGCACAGCACCA+GGG + chr1.4:10130259-10130278 None:intergenic 55.0%
CCGCTAACACGCATCTGATC+AGG + chr1.4:10130043-10130062 None:intergenic 55.0%
CGGTGAGTCTCCACATACCA+TGG + chr1.4:10131575-10131594 None:intergenic 55.0%
GACCTGTTGTCCGTCGTGTT+GGG - chr1.4:10130639-10130658 MS.gene021555:intron 55.0%
GATCGTAGTGGTAGAGGAGG+TGG - chr1.4:10131462-10131481 MS.gene021555:CDS 55.0%
GCAACTCTACACCCCACTCA+CGG + chr1.4:10130619-10130638 None:intergenic 55.0%
GCCCTTCTACGTCTTCCTTC+AGG + chr1.4:10131612-10131631 None:intergenic 55.0%
GCGAACAGATGGCGAAAGCT+TGG - chr1.4:10130090-10130109 MS.gene021555:CDS 55.0%
GCGCGTCTGACATGGTTATG+TGG - chr1.4:10132120-10132139 MS.gene021555:CDS 55.0%
GGACTGCTGCCACTAACAGA+TGG - chr1.4:10130660-10130679 MS.gene021555:intron 55.0%
GGGTTAAACCTTCAGCAGCG+AGG + chr1.4:10129974-10129993 None:intergenic 55.0%
TACCTGCAGCTCTCACGGAT+GGG - chr1.4:10130984-10131003 MS.gene021555:CDS 55.0%
TGGCAGGTACGAGCAACCAA+TGG - chr1.4:10130470-10130489 MS.gene021555:intron 55.0%
TTACCTGCAGCTCTCACGGA+TGG - chr1.4:10130983-10131002 MS.gene021555:CDS 55.0%
! GCTGCCAAAGTGCTCGATGA+AGG - chr1.4:10130188-10130207 MS.gene021555:intron 55.0%
! GGAGACTCACCGGCTACTAT+AGG - chr1.4:10131583-10131602 MS.gene021555:CDS 55.0%
! TTGGTGGATGTTCCGGTGGA+AGG - chr1.4:10131792-10131811 MS.gene021555:CDS 55.0%
!! AGGTTTGGTGGATGTTCCGG+TGG - chr1.4:10131788-10131807 MS.gene021555:CDS 55.0%
!! TGATGACGGTGTCCGTGAGT+GGG - chr1.4:10130604-10130623 MS.gene021555:intron 55.0%
CAGCGAGGGTAAGGCCTTCA+AGG + chr1.4:10129959-10129978 None:intergenic 60.0%
CAGCTCTCACGGATGGGACA+AGG - chr1.4:10130990-10131009 MS.gene021555:CDS 60.0%
CGACCTGTTGTCCGTCGTGT+TGG - chr1.4:10130638-10130657 MS.gene021555:intron 60.0%
CTCTGATGATCAGGCCTGCC+GGG - chr1.4:10131258-10131277 MS.gene021555:CDS 60.0%
GAAGACGTAGAAGGGCTGCG+TGG - chr1.4:10131616-10131635 MS.gene021555:CDS 60.0%
GCTCTGATGATCAGGCCTGC+CGG - chr1.4:10131257-10131276 MS.gene021555:CDS 60.0%
GGTTGCTTGCGCGTCTGACA+TGG - chr1.4:10132112-10132131 MS.gene021555:CDS 60.0%
GTCCCAACACGACGGACAAC+AGG + chr1.4:10130644-10130663 None:intergenic 60.0%
GTTGAGCCGGCCACCTAGAT+TGG + chr1.4:10130789-10130808 None:intergenic 60.0%
TGGCAGCAGTCCCAACACGA+CGG + chr1.4:10130652-10130671 None:intergenic 60.0%
! GATGACGGTGTCCGTGAGTG+GGG - chr1.4:10130605-10130624 MS.gene021555:intron 60.0%
!! CTGATGACGGTGTCCGTGAG+TGG - chr1.4:10130603-10130622 MS.gene021555:intron 60.0%
GGCCTTACCCTCGCTGCTGA+AGG - chr1.4:10129963-10129982 MS.gene021555:intron 65.0%
GTCCCATCCGTGAGAGCTGC+AGG + chr1.4:10130989-10131008 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 10129936 10132219 10129936 ID=MS.gene021555
chr1.4 mRNA 10129936 10132219 10129936 ID=MS.gene021555.t1;Parent=MS.gene021555
chr1.4 exon 10132012 10132219 10132012 ID=MS.gene021555.t1.exon1;Parent=MS.gene021555.t1
chr1.4 CDS 10132012 10132219 10132012 ID=cds.MS.gene021555.t1;Parent=MS.gene021555.t1
chr1.4 exon 10130980 10131843 10130980 ID=MS.gene021555.t1.exon2;Parent=MS.gene021555.t1
chr1.4 CDS 10130980 10131843 10130980 ID=cds.MS.gene021555.t1;Parent=MS.gene021555.t1
chr1.4 exon 10130058 10130203 10130058 ID=MS.gene021555.t1.exon3;Parent=MS.gene021555.t1
chr1.4 CDS 10130058 10130203 10130058 ID=cds.MS.gene021555.t1;Parent=MS.gene021555.t1
chr1.4 exon 10129936 10129965 10129936 ID=MS.gene021555.t1.exon4;Parent=MS.gene021555.t1
chr1.4 CDS 10129936 10129965 10129936 ID=cds.MS.gene021555.t1;Parent=MS.gene021555.t1
Gene Sequence

>MS.gene021555

ATGAACACTGATGCCCTTAACCTTGAAGGCCTTACCCTCGCTGCTGAAGGTTTAACCCTAAACCTGAATGACAAACCAGATACGACCCAAATTTTGGAACACTGCCTGATCAGATGCGTGTTAGCGGATAGAGAGATCAAGTTTGCCTATTTTAGCGAACAGATGGCGAAAGCTTGGAAACCTGTCAAACATGTTACGATTCTGCAAGGTGAGGAAGATCGCTACCTGTTTCAATTCCATCACAAAATGGATGCTGCCAAAGTGCTCGATGAAGGTCCATGGGTGTATGATAGTTTTCATATCGTTGTTGATAGAATTGCCCCTGGTGCTGTGCAACGTGTTGTGCCTCTAAACCATATTGATTTTTGGGTGCAAGTCCATGGTTTACCCTTTGATTTCATCCAACAAAGGGTTGGTCAAGGCATTGGTCAATTCCTTGGCGAAATGAAATTGTACGACAACCGTAACACAATTCACAGCTTCTTTATGCGGTTAAAGGTTAGAATTGACATCACTCGTCCAATGAAAAAACAGTGGCAGGTACGAGCAACCAATGGTGCCTATATTACTATTCACTTTAAGTACGAGAAGCTGGGAGTTTTATGCTATAGGTGTGGCCTTCTCGGTCATACTGACAAGGTATGTCCTGATCTATTTGAGTTGGATTCTGATGACGGTGTCCGTGAGTGGGGTGTAGAGTTGCGACCTGTTGTCCGTCGTGTTGGGACTGCTGCCACTAACAGATGGTTGCAAAACCCTATACCATCTGTTGTGCCGAGAACTGATAATGCTTTTGGAGGAACACATGTTGGTACTTCTAACACTGCTGATGATGGTAATGTGAATATTTCCAATCTAGGTGGCCGGCTCAACGCTCTGCATAATGAGATTAATTCTATCAATAATGGTATTATGATTGTTCAAAACAACTCGTTGGCCAAGAGTGGTAACGCTATTACTGCTGTGCATCTAAATTCTGCTCTTCCTTCCTCATCTGCGAGTACTTCTGCTACTGTTCTCAATATCTCCAAACCTGTTGTGCTGAGTTTACCTGCAGCTCTCACGGATGGGACAAGGGCTGAGAATGTTACTGCTCCTAATGAGTCTGAAGCTGAGGATATCAGGTCAGAAATGAAGAAGCGGAAGCTAATGTTGGCGGAATGTCTTCAAGGTGGTGCTCTTCAAAATGATATTGAGTTTTCTTTTGTTGTTGATGGGGTTAATGGGGATACTGAACTAGGTTATAATGTTAGTGAGAGGGGTGATATTGTAATGAATGTTTGTGACAATCCTTTGTTTCAAAGTAATGATGTATTGACAGGCTCTGATGATCAGGCCTGCCGGGATAAATGAGTCTGATAGGATGGAATTGCAGGGGTTTAGGTCGCCCGAATGCAATTCCAGATTTAAAGTACCTAGTTCGGCACTTCAATTCGGACCTCTTGTTCTTAAGCGAGACACTAGTCTCTCGCAATAAAATTGAAGATTTACGTTATTTGCTTGGTTTTGATTTTTGCTTTTCTGTTGATCGTAGTGGTAGAGGAGGTGGCCTTGCTCTTTTTTGGCGCACTTCTTTTAGTTGTCAAATTGTAGATTACTCAAATAATCATATTTATGTTGATGTTAATGATGTTGTCCATGGTATGTGGAGACTCACCGGCTACTATAGGTACCCTGAAGGAAGACGTAGAAGGGCTGCGTGGGATTTCCTTCGTCAATTATCTAATCAGTTTGCAGGTCCTTGGTGTATACTAGGCGACTTTAATGATATTATGGATGCTAGTGAAAAAAGGGGAAAAAATCTTCGACCTAACTGGTTGATAAATGGTTTCCGACAAGCGATTATTGATACAGGTTTGGTGGATGTTCCGGTGGAAGGATATCCTTTCACTTGGTTCAAAAGTTTGGGAACACCACGTGCGGTTGAAGAACGTCTTGATAGAGCCCTTGCCAATAATCATTGGTTTAATCTCTTTCCTACGGCTACTCTGGAGAATCTTGATGCCCCATCTTCAGATCACTATCCCATCCTTTTGAACAAATGTTTGATGCATCGTCCTCATCTTCAAAAGCGTGGTTTTCGATACGAAAATGCGTGGTATTTTGAACCAGGTTTCAAGGAGCTTGTTACTGATGCATGGCACACACAATTTGCATTTTCGGTAGTAGCTAAATTGGTTGCTTGCGCGTCTGACATGGTTATGTGGAGTAGGAAGCATTGCAATAAGCTGAAGGGTGATATTGAAGAGTGCAGGCGTCAGATTCAAAACATCAGGTTGAATAG
Protein sequence

>MS.gene021555.t1

MNTDALNLEGLTLAAEGLTLNLNDKPDTTQILEHCLIRCVLADREIKFAYFSEQMAKAWKPVKHVTILQVHGLPFDFIQQRVGQGIGQFLGEMKLYDNRNTIHSFFMRLKVRIDITRPMKKQWQVRATNGAYITIHFKYEKLGVLCYRCGLLGHTDKVCPDLFELDSDDGVREWGVELRPVVRRVGTAATNRWLQNPIPSVVPRTDNAFGGTHVGTSNTADDGNVNISNLGGRLNALHNEINSINNGIMIVQNNSLAKSGNAITAVHLNSALPSSSASTSATVLNISKPVVLSLPAALTDGTRAENVTAPNESEAEDIRSEMKKRKLMLAECLQGGALQNDIEFSFVVDGVNGDTELDHYPILLNKCLMHRPHLQKRGFRYENAWYFEPGFKELVTDAWHTQFAFSASDSKHQVE