AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81712

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81712.t1 MTR_6g033215 49.383 81 41 0 2 82 214 294 4.78e-22 94.4
MS.gene81712.t1 MTR_6g053230 51.429 70 34 0 1 70 101 170 3.70e-20 91.3
MS.gene81712.t1 MTR_8g468010 56.667 60 26 0 2 61 5 64 4.95e-20 85.1
MS.gene81712.t1 MTR_1g032270 44.118 68 38 0 2 69 22 89 3.59e-14 69.3
MS.gene81712.t1 MTR_3g024240 43.210 81 44 1 5 83 95 175 7.21e-14 69.3
MS.gene81712.t1 MTR_0010s0370 48.276 58 30 0 4 61 121 178 4.36e-13 67.4
MS.gene81712.t1 MTR_0010s0350 48.276 58 30 0 4 61 121 178 4.36e-13 67.4
MS.gene81712.t1 MTR_7g089990 34.737 95 60 1 5 97 104 198 2.15e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81712.t1 AT2G17920 25.743 101 75 0 1 101 121 221 1.41e-11 64.7

Find 82 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCACCAACCTTGCAATTC+TGG 0.253069 1.3:-44025457 None:intergenic
CAAGCTCATAACCTCCCTTT+TGG 0.272767 1.3:+44024763 MS.gene81712:CDS
CCTGCTAGTTGAAGTATGTT+TGG 0.317869 1.3:-44025387 None:intergenic
TTCTGAAATGCGTGCTGAAC+AGG 0.318069 1.3:+44025422 MS.gene81712:CDS
CAGCGGTTCATTGACATTCA+TGG 0.329671 1.3:-44024899 None:intergenic
CTGCTAGTTGAAGTATGTTT+GGG 0.330826 1.3:-44025386 None:intergenic
GGCAATTTCTGTTATATTTG+CGG 0.333323 1.3:+44024991 MS.gene81712:CDS
CTTAATTAACCCTCAACCTA+TGG 0.345720 1.3:+44025560 MS.gene81712:CDS
CGAAGCCGGGTTGATGATTC+AGG 0.346012 1.3:+44025654 MS.gene81712:CDS
TGGATTTATGGCTGAATCAA+TGG 0.356903 1.3:+44024783 MS.gene81712:CDS
TGCACTTGTTGCTTAAAATC+AGG 0.369103 1.3:-44026586 None:intergenic
TGATATTTGCAAAATACATT+GGG 0.388096 1.3:+44025268 MS.gene81712:CDS
TGAATCAATGGGCCATCTCT+TGG 0.389213 1.3:+44024795 MS.gene81712:CDS
CTTACCTGCCCCATAGGTTG+AGG 0.403026 1.3:-44025570 None:intergenic
ATATTGACACGATGGACATA+TGG 0.408857 1.3:+44024737 None:intergenic
TGACATTCATGGTAACTCTA+AGG 0.409712 1.3:-44024888 None:intergenic
TGAACCGCTGAAGCAGTGTT+TGG 0.422967 1.3:+44024912 MS.gene81712:CDS
GGATTTATGGCTGAATCAAT+GGG 0.424684 1.3:+44024784 MS.gene81712:CDS
TGATGATTCAGGTGCTGAAA+TGG 0.438586 1.3:+44025665 MS.gene81712:CDS
GAATCAATGGGCCATCTCTT+GGG 0.444007 1.3:+44024796 MS.gene81712:CDS
TAAAACAGCTGAAGCGAAGC+CGG 0.457929 1.3:+44025640 MS.gene81712:CDS
AATATCAGCTCCTTTGTGAC+AGG 0.460063 1.3:-44025252 None:intergenic
AAAACAGCTGAAGCGAAGCC+GGG 0.462216 1.3:+44025641 MS.gene81712:CDS
TGTTATATTTGCGGATTGCT+AGG 0.463465 1.3:+44025000 MS.gene81712:CDS
CTGATATTTGCAAAATACAT+TGG 0.466883 1.3:+44025267 MS.gene81712:CDS
CTCAACCTATGGGGCAGGTA+AGG 0.470443 1.3:+44025571 MS.gene81712:CDS
TGAAAGGGAAGGTGCTGATC+CGG 0.470597 1.3:+44024939 MS.gene81712:CDS
GAAGTCGCTGCTGCTGCACC+AGG 0.471264 1.3:+44025363 MS.gene81712:CDS
TTAATTAACCCTCAACCTAT+GGG 0.482092 1.3:+44025561 MS.gene81712:CDS
CAACATGGTTTCCCAAGAGA+TGG 0.483692 1.3:-44024807 None:intergenic
CACAAAACAGCAGCAATGAA+TGG 0.483837 1.3:+44025292 MS.gene81712:CDS
GATTCAGCCATAAATCCAAA+AGG 0.488076 1.3:-44024778 None:intergenic
AGCAGTGTTTGGAATTTGAA+AGG 0.492894 1.3:+44024923 MS.gene81712:CDS
AGCAACAAGTGCAGCAACGC+TGG 0.496058 1.3:+44026596 MS.gene81712:CDS
ATACTTCAACTAGCAGGAAC+GGG 0.496717 1.3:+44025393 MS.gene81712:CDS
AGTTGAAGTATGTTTGGGCC+TGG 0.497725 1.3:-44025381 None:intergenic
TTACCTGCCCCATAGGTTGA+GGG 0.506413 1.3:-44025569 None:intergenic
GATGCTGTTCAGACAAACAT+AGG 0.507036 1.3:+44025702 MS.gene81712:CDS
TTAACAACAATTATGGTACA+TGG 0.508600 1.3:+44024851 MS.gene81712:CDS
ACTGTGGCTCACTTGCAAAT+CGG 0.508959 1.3:+44026504 MS.gene81712:CDS
TCAGTGCAGATTTCAATGCT+TGG 0.509237 1.3:+44025331 MS.gene81712:CDS
CGAGTCGGAAAAGTCAAGAT+TGG 0.520583 1.3:+44025222 MS.gene81712:CDS
CATTGAAATCTGCACTGAAA+GGG 0.528617 1.3:-44025326 None:intergenic
TGCTGAACAGGAAGAGGATG+AGG 0.535578 1.3:+44025434 MS.gene81712:CDS
GTGCACAGAGGCAGAAAGGT+GGG 0.535950 1.3:+44025169 MS.gene81712:CDS
ACAGGTGCACAGAGGCAGAA+AGG 0.536033 1.3:+44025165 MS.gene81712:CDS
AAAATAAGCACAAAAGATGA+TGG 0.536135 1.3:+44025615 MS.gene81712:CDS
GCATTGAAATCTGCACTGAA+AGG 0.538168 1.3:-44025327 None:intergenic
TAACCCTCAACCTATGGGGC+AGG 0.540726 1.3:+44025566 MS.gene81712:CDS
GCAGTGTTTGGAATTTGAAA+GGG 0.541766 1.3:+44024924 MS.gene81712:CDS
GCAGCAGCGACTTCCACGAC+TGG 0.547541 1.3:-44025354 None:intergenic
TTTCAATGCTTGGCCAGTCG+TGG 0.548634 1.3:+44025341 MS.gene81712:CDS
AATTCCAAACACTGCTTCAG+CGG 0.549989 1.3:-44024916 None:intergenic
CTATGAATCTTACTGCAAGC+TGG 0.562630 1.3:-44025198 None:intergenic
TGTTCAGCACGCATTTCAGA+AGG 0.563581 1.3:-44025420 None:intergenic
ATTCAGCCATAAATCCAAAA+GGG 0.564283 1.3:-44024777 None:intergenic
GTCATCCTTACCTGCCCCAT+AGG 0.567245 1.3:-44025576 None:intergenic
TGGAAGCTCTATCCTGAAGA+AGG 0.567863 1.3:+44026616 MS.gene81712:CDS
TGGCTGTGAAGACAAGAGAC+AGG 0.572345 1.3:+44025147 MS.gene81712:intron
CATCTCTTGGGAAACCATGT+TGG 0.576252 1.3:+44024808 MS.gene81712:CDS
CATACTTCAACTAGCAGGAA+CGG 0.576272 1.3:+44025392 MS.gene81712:CDS
CCAAACATACTTCAACTAGC+AGG 0.590414 1.3:+44025387 MS.gene81712:CDS
AATGCGTGCTGAACAGGAAG+AGG 0.606906 1.3:+44025428 MS.gene81712:CDS
GCTTTAAGTTTGAAAATGCG+TGG 0.609787 1.3:+44026554 MS.gene81712:CDS
AGTAAGATTCATAGTCGAGT+CGG 0.614668 1.3:+44025207 MS.gene81712:CDS
GATCAGAAGATGTCGTCGAA+AGG 0.615542 1.3:-44026474 None:intergenic
ATATCAGCTCCTTTGTGACA+GGG 0.617189 1.3:-44025251 None:intergenic
AGGTGCTGATCCGGTGACCG+TGG 0.620417 1.3:+44024948 MS.gene81712:CDS
TGTTTGGAATTTGAAAGGGA+AGG 0.622070 1.3:+44024928 MS.gene81712:CDS
TGCACAGAGGCAGAAAGGTG+GGG 0.622762 1.3:+44025170 MS.gene81712:CDS
AAATATAACAGAAATTGCCA+AGG 0.630713 1.3:-44024987 None:intergenic
CAGCACCTGAATCATCAACC+CGG 0.638976 1.3:-44025659 None:intergenic
GGTGCACAGAGGCAGAAAGG+TGG 0.647658 1.3:+44025168 MS.gene81712:CDS
TGGTAGAAACCCTGTCACAA+AGG 0.649175 1.3:+44025242 MS.gene81712:CDS
CAGCCATAAATCCAAAAGGG+AGG 0.660827 1.3:-44024774 None:intergenic
GATATTTGCAAAATACATTG+GGG 0.667589 1.3:+44025269 MS.gene81712:CDS
ATTTGCAAGTGAGCCACAGT+GGG 0.668730 1.3:-44026501 None:intergenic
TAATTAACCCTCAACCTATG+GGG 0.689151 1.3:+44025562 MS.gene81712:CDS
AGGATGAGGCCAGAATTGCA+AGG 0.696977 1.3:+44025448 MS.gene81712:CDS
GACAAGAGACAGGTGCACAG+AGG 0.697714 1.3:+44025157 MS.gene81712:intron
GATTTGCAAGTGAGCCACAG+TGG 0.703283 1.3:-44026502 None:intergenic
CTTCTGATCATTACCCACTG+TGG 0.743178 1.3:+44026488 MS.gene81712:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGATTTTAACAACAATTA+TGG + chr1.3:44024844-44024863 MS.gene81712:CDS 15.0%
!! TGATATTTGCAAAATACATT+GGG + chr1.3:44025268-44025287 MS.gene81712:CDS 20.0%
! AAAATAAGCACAAAAGATGA+TGG + chr1.3:44025615-44025634 MS.gene81712:CDS 25.0%
! AAATATAACAGAAATTGCCA+AGG - chr1.3:44024990-44025009 None:intergenic 25.0%
! CTGATATTTGCAAAATACAT+TGG + chr1.3:44025267-44025286 MS.gene81712:CDS 25.0%
! GATATTTGCAAAATACATTG+GGG + chr1.3:44025269-44025288 MS.gene81712:CDS 25.0%
! GCTTTCTTAAAAAATCTAAC+AGG + chr1.3:44026181-44026200 MS.gene81712:intron 25.0%
! TTAACAACAATTATGGTACA+TGG + chr1.3:44024851-44024870 MS.gene81712:CDS 25.0%
!! AATATTTCTGTCTGAAACAA+TGG + chr1.3:44025919-44025938 MS.gene81712:intron 25.0%
!! ACAATAAAACTCATTTTCCT+TGG - chr1.3:44025821-44025840 None:intergenic 25.0%
!!! GGAAAATGAGTTTTATTGTA+TGG + chr1.3:44025822-44025841 MS.gene81712:intron 25.0%
!!! TTTTCAAACTTAAAGCGTTT+TGG - chr1.3:44026550-44026569 None:intergenic 25.0%
!!! TTTTGTTATTGCATAAGAAC+AGG - chr1.3:44025522-44025541 None:intergenic 25.0%
AAATCACATTGATGTTGAAG+TGG + chr1.3:44026081-44026100 MS.gene81712:intron 30.0%
AGTTTGAAAGCTGAATTTCA+AGG + chr1.3:44025093-44025112 MS.gene81712:intron 30.0%
ATTCAGCCATAAATCCAAAA+GGG - chr1.3:44024780-44024799 None:intergenic 30.0%
GGCAATTTCTGTTATATTTG+CGG + chr1.3:44024991-44025010 MS.gene81712:CDS 30.0%
GTTTGAAAGCGATAATGTAA+TGG + chr1.3:44025773-44025792 MS.gene81712:intron 30.0%
TGAAATTCAGCTTTCAAACT+TGG - chr1.3:44025093-44025112 None:intergenic 30.0%
TTAATTAACCCTCAACCTAT+GGG + chr1.3:44025561-44025580 MS.gene81712:CDS 30.0%
TTTGAACTTTACTGATGTCA+CGG - chr1.3:44026711-44026730 None:intergenic 30.0%
! ATAAAACTCATTTTCCTTGG+CGG - chr1.3:44025818-44025837 None:intergenic 30.0%
! GAATCTCCATAGTTTGTATT+GGG - chr1.3:44025500-44025519 None:intergenic 30.0%
! GTTTTCGTACTTAAAAACCA+CGG - chr1.3:44024968-44024987 None:intergenic 30.0%
! TCTTTTCTTCTTCCATTTTC+CGG - chr1.3:44026154-44026173 None:intergenic 30.0%
! TTTGAAGCAGGATTTATTGA+AGG + chr1.3:44025051-44025070 MS.gene81712:intron 30.0%
! TTTTATTGTATGGAACTGTC+GGG + chr1.3:44025832-44025851 MS.gene81712:intron 30.0%
!! AAGTGGATGATTTGTTGATT+GGG + chr1.3:44026098-44026117 MS.gene81712:intron 30.0%
!! GTTTTATTGTATGGAACTGT+CGG + chr1.3:44025831-44025850 MS.gene81712:intron 30.0%
!!! GTCTTTCGATTCTTGTTTTA+CGG + chr1.3:44025979-44025998 MS.gene81712:intron 30.0%
!!! GTTTTTAAGTACGAAAACCT+TGG + chr1.3:44024970-44024989 MS.gene81712:CDS 30.0%
!!! TCTTTTGTGCTTATTTTGAG+TGG - chr1.3:44025612-44025631 None:intergenic 30.0%
!!! TGTTTTACGGTTGATAGAAT+TGG + chr1.3:44025992-44026011 MS.gene81712:intron 30.0%
AGTAAGATTCATAGTCGAGT+CGG + chr1.3:44025207-44025226 MS.gene81712:CDS 35.0%
ATGGAAGAAGAAAAGAATCG+TGG + chr1.3:44026158-44026177 MS.gene81712:intron 35.0%
CATTGAAATCTGCACTGAAA+GGG - chr1.3:44025329-44025348 None:intergenic 35.0%
CTGCTAGTTGAAGTATGTTT+GGG - chr1.3:44025389-44025408 None:intergenic 35.0%
CTTAATTAACCCTCAACCTA+TGG + chr1.3:44025560-44025579 MS.gene81712:CDS 35.0%
GAAGAACCCAATACAAACTA+TGG + chr1.3:44025491-44025510 MS.gene81712:intron 35.0%
GAGCACTTGTAAATTCTGAT+TGG + chr1.3:44026419-44026438 MS.gene81712:intron 35.0%
GATTCAGCCATAAATCCAAA+AGG - chr1.3:44024781-44024800 None:intergenic 35.0%
GGATTTATGGCTGAATCAAT+GGG + chr1.3:44024784-44024803 MS.gene81712:CDS 35.0%
TAATTAACCCTCAACCTATG+GGG + chr1.3:44025562-44025581 MS.gene81712:CDS 35.0%
TATTACGACTCCAATCAATC+AGG - chr1.3:44026675-44026694 None:intergenic 35.0%
TATTGAAGGACCAAAGAAGT+GGG + chr1.3:44025065-44025084 MS.gene81712:intron 35.0%
TCAGACAGAAATATTCCTTC+CGG - chr1.3:44025914-44025933 None:intergenic 35.0%
TGAAAGCGATAATGTAATGG+CGG + chr1.3:44025776-44025795 MS.gene81712:intron 35.0%
TGACATTCATGGTAACTCTA+AGG - chr1.3:44024891-44024910 None:intergenic 35.0%
TGGAAGAAGAAAAGAATCGT+GGG + chr1.3:44026159-44026178 MS.gene81712:intron 35.0%
TGGATTTATGGCTGAATCAA+TGG + chr1.3:44024783-44024802 MS.gene81712:CDS 35.0%
TTATTGAAGGACCAAAGAAG+TGG + chr1.3:44025064-44025083 MS.gene81712:intron 35.0%
TTCACTTGGTTCAAAAGTCT+CGG + chr1.3:44026364-44026383 MS.gene81712:intron 35.0%
TTCTCTAGCAATAGATAGTC+AGG + chr1.3:44025725-44025744 MS.gene81712:intron 35.0%
TTGTCATCTGAAGAGAAGAA+AGG + chr1.3:44026250-44026269 MS.gene81712:intron 35.0%
TTTATTGTATGGAACTGTCG+GGG + chr1.3:44025833-44025852 MS.gene81712:intron 35.0%
! AAATCCTGCTTCAAACCTTT+TGG - chr1.3:44025046-44025065 None:intergenic 35.0%
! AATCCTGCTTCAAACCTTTT+GGG - chr1.3:44025045-44025064 None:intergenic 35.0%
! AGCAGTGTTTGGAATTTGAA+AGG + chr1.3:44024923-44024942 MS.gene81712:CDS 35.0%
! ATTTCAGCAGTTTTCCAACA+TGG - chr1.3:44024825-44024844 None:intergenic 35.0%
! GCAGTGTTTGGAATTTGAAA+GGG + chr1.3:44024924-44024943 MS.gene81712:CDS 35.0%
! GCTTTAAGTTTGAAAATGCG+TGG + chr1.3:44026554-44026573 MS.gene81712:CDS 35.0%
! GGAATCTCCATAGTTTGTAT+TGG - chr1.3:44025501-44025520 None:intergenic 35.0%
! TAAAACTCATTTTCCTTGGC+GGG - chr1.3:44025817-44025836 None:intergenic 35.0%
! TAACCTCCCTTTTGGATTTA+TGG + chr1.3:44024771-44024790 MS.gene81712:CDS 35.0%
! TGCACTTGTTGCTTAAAATC+AGG - chr1.3:44026589-44026608 None:intergenic 35.0%
! TGTTATATTTGCGGATTGCT+AGG + chr1.3:44025000-44025019 MS.gene81712:CDS 35.0%
! TGTTTGGAATTTGAAAGGGA+AGG + chr1.3:44024928-44024947 MS.gene81712:CDS 35.0%
!! GAAGTGGATGATTTGTTGAT+TGG + chr1.3:44026097-44026116 MS.gene81712:intron 35.0%
!! TACGGTTGATAGAATTGGTA+GGG + chr1.3:44025997-44026016 MS.gene81712:intron 35.0%
!! TTACGGTTGATAGAATTGGT+AGG + chr1.3:44025996-44026015 MS.gene81712:intron 35.0%
!! TTTTGAGTGATACCTTCTTC+AGG - chr1.3:44026631-44026650 None:intergenic 35.0%
!!! AGCGATTGACTGGTTTTTAT+GGG + chr1.3:44026125-44026144 MS.gene81712:intron 35.0%
!!! TAGCGATTGACTGGTTTTTA+TGG + chr1.3:44026124-44026143 MS.gene81712:intron 35.0%
AATATCAGCTCCTTTGTGAC+AGG - chr1.3:44025255-44025274 None:intergenic 40.0%
AATTCCAAACACTGCTTCAG+CGG - chr1.3:44024919-44024938 None:intergenic 40.0%
ACTCCAATCAATCAGGTCTT+CGG - chr1.3:44026668-44026687 None:intergenic 40.0%
AGGTTGAGGGTTAATTAAGC+TGG - chr1.3:44025559-44025578 None:intergenic 40.0%
ATACTTCAACTAGCAGGAAC+GGG + chr1.3:44025393-44025412 MS.gene81712:CDS 40.0%
ATATCAGCTCCTTTGTGACA+GGG - chr1.3:44025254-44025273 None:intergenic 40.0%
ATTGAAGGACCAAAGAAGTG+GGG + chr1.3:44025066-44025085 MS.gene81712:intron 40.0%
ATTGGGAAATAGCGATTGAC+TGG + chr1.3:44026115-44026134 MS.gene81712:intron 40.0%
CACAAAACAGCAGCAATGAA+TGG + chr1.3:44025292-44025311 MS.gene81712:CDS 40.0%
CATACTTCAACTAGCAGGAA+CGG + chr1.3:44025392-44025411 MS.gene81712:CDS 40.0%
CATCTGAAGAGAAGAAAGGT+AGG + chr1.3:44026254-44026273 MS.gene81712:intron 40.0%
CCAAACATACTTCAACTAGC+AGG + chr1.3:44025387-44025406 MS.gene81712:CDS 40.0%
CCTGCTAGTTGAAGTATGTT+TGG - chr1.3:44025390-44025409 None:intergenic 40.0%
CTATGAATCTTACTGCAAGC+TGG - chr1.3:44025201-44025220 None:intergenic 40.0%
GATGCTGTTCAGACAAACAT+AGG + chr1.3:44025702-44025721 MS.gene81712:CDS 40.0%
GCATTGAAATCTGCACTGAA+AGG - chr1.3:44025330-44025349 None:intergenic 40.0%
TACTTAAAAACCACGGTCAC+CGG - chr1.3:44024961-44024980 None:intergenic 40.0%
TCAGTGCAGATTTCAATGCT+TGG + chr1.3:44025331-44025350 MS.gene81712:CDS 40.0%
TGTAACATGTTCCCAAATGC+AGG + chr1.3:44026442-44026461 MS.gene81712:intron 40.0%
! ATCCTGCTTCAAACCTTTTG+GGG - chr1.3:44025044-44025063 None:intergenic 40.0%
! CTGTTTGACTTACACATGAC+TGG + chr1.3:44026334-44026353 MS.gene81712:intron 40.0%
! TGACTGGTTATGCTTTCACT+TGG + chr1.3:44026350-44026369 MS.gene81712:intron 40.0%
! TTTTATGGGATGCCGGAAAA+TGG + chr1.3:44026139-44026158 MS.gene81712:intron 40.0%
!! ACGGTTGATAGAATTGGTAG+GGG + chr1.3:44025998-44026017 MS.gene81712:intron 40.0%
!! TGATGATTCAGGTGCTGAAA+TGG + chr1.3:44025665-44025684 MS.gene81712:CDS 40.0%
AAACTTGGTCCCCACTTCTT+TGG - chr1.3:44025078-44025097 None:intergenic 45.0%
AAATCTCCGATAACGCACCA+TGG - chr1.3:44026220-44026239 None:intergenic 45.0%
ACTGTGGCTCACTTGCAAAT+CGG + chr1.3:44026504-44026523 MS.gene81712:CDS 45.0%
AGGCATTCAACACCTGCATT+TGG - chr1.3:44026457-44026476 None:intergenic 45.0%
AGTTGAAGTATGTTTGGGCC+TGG - chr1.3:44025384-44025403 None:intergenic 45.0%
ATGCACCAACCTTGCAATTC+TGG - chr1.3:44025460-44025479 None:intergenic 45.0%
ATTTGCAAGTGAGCCACAGT+GGG - chr1.3:44026504-44026523 None:intergenic 45.0%
CAACATGGTTTCCCAAGAGA+TGG - chr1.3:44024810-44024829 None:intergenic 45.0%
CAGCCATAAATCCAAAAGGG+AGG - chr1.3:44024777-44024796 None:intergenic 45.0%
CAGCGGTTCATTGACATTCA+TGG - chr1.3:44024902-44024921 None:intergenic 45.0%
CAGGAACTTCTCCTTTACCA+TGG + chr1.3:44026200-44026219 MS.gene81712:intron 45.0%
CATCTCTTGGGAAACCATGT+TGG + chr1.3:44024808-44024827 MS.gene81712:CDS 45.0%
CCTTTACCATGGTGCGTTAT+CGG + chr1.3:44026211-44026230 MS.gene81712:intron 45.0%
CGAGTCGGAAAAGTCAAGAT+TGG + chr1.3:44025222-44025241 MS.gene81712:CDS 45.0%
CTTCTGATCATTACCCACTG+TGG + chr1.3:44026488-44026507 MS.gene81712:CDS 45.0%
GAATCAATGGGCCATCTCTT+GGG + chr1.3:44024796-44024815 MS.gene81712:CDS 45.0%
GAATTTCAAGGCCCTCAAGA+AGG + chr1.3:44025105-44025124 MS.gene81712:intron 45.0%
GATCAGAAGATGTCGTCGAA+AGG - chr1.3:44026477-44026496 None:intergenic 45.0%
GGCATTCAACACCTGCATTT+GGG - chr1.3:44026456-44026475 None:intergenic 45.0%
TAAAACAGCTGAAGCGAAGC+CGG + chr1.3:44025640-44025659 MS.gene81712:CDS 45.0%
TAGCTTCTCTTCAACAGCTC+TGG - chr1.3:44026395-44026414 None:intergenic 45.0%
TGAACACTCCTCCTTCTTGA+GGG - chr1.3:44025119-44025138 None:intergenic 45.0%
TGAATCAATGGGCCATCTCT+TGG + chr1.3:44024795-44024814 MS.gene81712:CDS 45.0%
TGGAAGCTCTATCCTGAAGA+AGG + chr1.3:44026616-44026635 MS.gene81712:CDS 45.0%
TGGTAGAAACCCTGTCACAA+AGG + chr1.3:44025242-44025261 MS.gene81712:CDS 45.0%
TGTTCAGCACGCATTTCAGA+AGG - chr1.3:44025423-44025442 None:intergenic 45.0%
! CAAGCTCATAACCTCCCTTT+TGG + chr1.3:44024763-44024782 MS.gene81712:CDS 45.0%
! CGGACAAGGTATTTCAGAGT+GGG - chr1.3:44025881-44025900 None:intergenic 45.0%
! GTTGATAGAATTGGTAGGGG+AGG + chr1.3:44026001-44026020 MS.gene81712:intron 45.0%
! TCGGACAAGGTATTTCAGAG+TGG - chr1.3:44025882-44025901 None:intergenic 45.0%
! TTCCTTCCGGTTCGTATGTT+CGG - chr1.3:44025901-44025920 None:intergenic 45.0%
!! CTGATGCTTTTTGCCCCAAA+AGG + chr1.3:44025028-44025047 MS.gene81712:CDS 45.0%
!! GAGGAGTGTTCACAAATCCT+TGG + chr1.3:44025127-44025146 MS.gene81712:intron 45.0%
!! TTCTGAAATGCGTGCTGAAC+AGG + chr1.3:44025422-44025441 MS.gene81712:CDS 45.0%
!!! GACTGGTTTTTATGGGATGC+CGG + chr1.3:44026132-44026151 MS.gene81712:intron 45.0%
AAAACAGCTGAAGCGAAGCC+GGG + chr1.3:44025641-44025660 MS.gene81712:CDS 50.0%
AATGCGTGCTGAACAGGAAG+AGG + chr1.3:44025428-44025447 MS.gene81712:CDS 50.0%
ACACTGCTTGCCTGAAACCA+CGG - chr1.3:44026303-44026322 None:intergenic 50.0%
AGGATGAGGCCAGAATTGCA+AGG + chr1.3:44025448-44025467 MS.gene81712:CDS 50.0%
CAGCACCTGAATCATCAACC+CGG - chr1.3:44025662-44025681 None:intergenic 50.0%
CCGATAACGCACCATGGTAA+AGG - chr1.3:44026214-44026233 None:intergenic 50.0%
CCTTGTCCGAACATACGAAC+CGG + chr1.3:44025892-44025911 MS.gene81712:intron 50.0%
GATAGAATTGGTAGGGGAGG+AGG + chr1.3:44026004-44026023 MS.gene81712:intron 50.0%
GATTTGCAAGTGAGCCACAG+TGG - chr1.3:44026505-44026524 None:intergenic 50.0%
GTCTCTTGTCTTCACAGCCA+AGG - chr1.3:44025147-44025166 None:intergenic 50.0%
GTGAACACTCCTCCTTCTTG+AGG - chr1.3:44025120-44025139 None:intergenic 50.0%
TGAACCGCTGAAGCAGTGTT+TGG + chr1.3:44024912-44024931 MS.gene81712:CDS 50.0%
TGAGGCCAGAATTGCAAGGT+TGG + chr1.3:44025452-44025471 MS.gene81712:intron 50.0%
TGCTGAACAGGAAGAGGATG+AGG + chr1.3:44025434-44025453 MS.gene81712:CDS 50.0%
TGGCTGTGAAGACAAGAGAC+AGG + chr1.3:44025147-44025166 MS.gene81712:intron 50.0%
TGGTTTCAGGCAAGCAGTGT+TGG + chr1.3:44026303-44026322 MS.gene81712:intron 50.0%
TTCAGAGTGGGAACTGTGCT+CGG - chr1.3:44025869-44025888 None:intergenic 50.0%
TTTCAAGGCCCTCAAGAAGG+AGG + chr1.3:44025108-44025127 MS.gene81712:intron 50.0%
TTTCAATGCTTGGCCAGTCG+TGG + chr1.3:44025341-44025360 MS.gene81712:CDS 50.0%
! CTTCTCTTCAACAGCTCTGG+CGG - chr1.3:44026392-44026411 None:intergenic 50.0%
! GATAATGTAATGGCGGAGCC+TGG + chr1.3:44025783-44025802 MS.gene81712:intron 50.0%
! TGCCCCAAAAGGTTTGAAGC+AGG + chr1.3:44025039-44025058 MS.gene81712:intron 50.0%
! TTACCTGCCCCATAGGTTGA+GGG - chr1.3:44025572-44025591 None:intergenic 50.0%
!! GAGGAGGCTTGGCTTTTCTT+TGG + chr1.3:44026020-44026039 MS.gene81712:intron 50.0%
!! TGAAAGGGAAGGTGCTGATC+CGG + chr1.3:44024939-44024958 MS.gene81712:CDS 50.0%
!! TGGGGCAAAAAGCATCAGTG+TGG - chr1.3:44025026-44025045 None:intergenic 50.0%
AATTGGTAGGGGAGGAGGCT+TGG + chr1.3:44026009-44026028 MS.gene81712:intron 55.0%
ACAGGTGCACAGAGGCAGAA+AGG + chr1.3:44025165-44025184 MS.gene81712:CDS 55.0%
AGCAACAAGTGCAGCAACGC+TGG + chr1.3:44026596-44026615 MS.gene81712:CDS 55.0%
CTCAACCTATGGGGCAGGTA+AGG + chr1.3:44025571-44025590 MS.gene81712:CDS 55.0%
CTGAAACCACGGATCAGCCA+AGG - chr1.3:44026292-44026311 None:intergenic 55.0%
CTTACCTGCCCCATAGGTTG+AGG - chr1.3:44025573-44025592 None:intergenic 55.0%
CTTGGCTGATCCGTGGTTTC+AGG + chr1.3:44026290-44026309 MS.gene81712:intron 55.0%
GACAAGAGACAGGTGCACAG+AGG + chr1.3:44025157-44025176 MS.gene81712:intron 55.0%
GCGCCGAAGACCTGATTGAT+TGG + chr1.3:44026662-44026681 MS.gene81712:intron 55.0%
GTCATCCTTACCTGCCCCAT+AGG - chr1.3:44025579-44025598 None:intergenic 55.0%
GTCCGAACATACGAACCGGA+AGG + chr1.3:44025896-44025915 MS.gene81712:intron 55.0%
GTGCACAGAGGCAGAAAGGT+GGG + chr1.3:44025169-44025188 MS.gene81712:CDS 55.0%
TAACCCTCAACCTATGGGGC+AGG + chr1.3:44025566-44025585 MS.gene81712:CDS 55.0%
TCAGAGTGGGAACTGTGCTC+GGG - chr1.3:44025868-44025887 None:intergenic 55.0%
TGCACAGAGGCAGAAAGGTG+GGG + chr1.3:44025170-44025189 MS.gene81712:CDS 55.0%
! CCGGTTCGTATGTTCGGACA+AGG - chr1.3:44025895-44025914 None:intergenic 55.0%
!! CGAAGCCGGGTTGATGATTC+AGG + chr1.3:44025654-44025673 MS.gene81712:CDS 55.0%
GGTGCACAGAGGCAGAAAGG+TGG + chr1.3:44025168-44025187 MS.gene81712:CDS 60.0%
GTATGGAACTGTCGGGGCCT+GGG + chr1.3:44025839-44025858 MS.gene81712:intron 60.0%
TGTATGGAACTGTCGGGGCC+TGG + chr1.3:44025838-44025857 MS.gene81712:intron 60.0%
! AGGCAAGCAGTGTTGGACGC+GGG + chr1.3:44026310-44026329 MS.gene81712:intron 60.0%
! CAGGCAAGCAGTGTTGGACG+CGG + chr1.3:44026309-44026328 MS.gene81712:intron 60.0%
!! CTCATTTTCCTTGGCGGGCC+TGG - chr1.3:44025812-44025831 None:intergenic 60.0%
!! GTAGGTCAGAGAGAGCGCCT+TGG + chr1.3:44026272-44026291 MS.gene81712:intron 60.0%
GAAGTCGCTGCTGCTGCACC+AGG + chr1.3:44025363-44025382 MS.gene81712:CDS 65.0%
GCAGCAGCGACTTCCACGAC+TGG - chr1.3:44025357-44025376 None:intergenic 65.0%
! AATGGCGGAGCCTGGTCACC+AGG + chr1.3:44025791-44025810 MS.gene81712:intron 65.0%
! AGAGCGCCTTGGCTGATCCG+TGG + chr1.3:44026283-44026302 MS.gene81712:intron 65.0%
!! AGGTGCTGATCCGGTGACCG+TGG + chr1.3:44024948-44024967 MS.gene81712:CDS 65.0%
GAACTGTGCTCGGGCTGCCC+AGG - chr1.3:44025859-44025878 None:intergenic 70.0%
CCTGGTCACCAGGCCCGCCA+AGG + chr1.3:44025801-44025820 MS.gene81712:intron 75.0%
! CCTTGGCGGGCCTGGTGACC+AGG - chr1.3:44025804-44025823 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 44024748 44026739 44024748 ID=MS.gene81712
chr1.3 mRNA 44024748 44026739 44024748 ID=MS.gene81712.t1;Parent=MS.gene81712
chr1.3 exon 44024748 44025049 44024748 ID=MS.gene81712.t1.exon1;Parent=MS.gene81712.t1
chr1.3 CDS 44024748 44025049 44024748 ID=cds.MS.gene81712.t1;Parent=MS.gene81712.t1
chr1.3 exon 44025158 44025469 44025158 ID=MS.gene81712.t1.exon2;Parent=MS.gene81712.t1
chr1.3 CDS 44025158 44025469 44025158 ID=cds.MS.gene81712.t1;Parent=MS.gene81712.t1
chr1.3 exon 44025560 44025723 44025560 ID=MS.gene81712.t1.exon3;Parent=MS.gene81712.t1
chr1.3 CDS 44025560 44025723 44025560 ID=cds.MS.gene81712.t1;Parent=MS.gene81712.t1
chr1.3 exon 44026464 44026637 44026464 ID=MS.gene81712.t1.exon4;Parent=MS.gene81712.t1
chr1.3 CDS 44026464 44026637 44026464 ID=cds.MS.gene81712.t1;Parent=MS.gene81712.t1
chr1.3 exon 44026720 44026739 44026720 ID=MS.gene81712.t1.exon5;Parent=MS.gene81712.t1
chr1.3 CDS 44026720 44026739 44026720 ID=cds.MS.gene81712.t1;Parent=MS.gene81712.t1
Gene Sequence

>MS.gene81712

ATGGACATATGGATTCAAGCTCATAACCTCCCTTTTGGATTTATGGCTGAATCAATGGGCCATCTCTTGGGAAACCATGTTGGAAAACTGCTGAAATATGATTTTAACAACAATTATGGTACATGGCGTAAATACATGCGCCTTAGAGTTACCATGAATGTCAATGAACCGCTGAAGCAGTGTTTGGAATTTGAAAGGGAAGGTGCTGATCCGGTGACCGTGGTTTTTAAGTACGAAAACCTTGGCAATTTCTGTTATATTTGCGGATTGCTAGGCCACACTGATGCTTTTTGCCCCAAAAGGTTTGAAGCAGGATTTATTGAAGGACCAAAGAAGTGGGGACCAAGTTTGAAAGCTGAATTTCAAGGCCCTCAAGAAGGAGGAGTGTTCACAAATCCTTGGCTGTGAAGACAAGAGACAGGTGCACAGAGGCAGAAAGGTGGGGAACGTCCAGCTTGCAGTAAGATTCATAGTCGAGTCGGAAAAGTCAAGATTGGTAGAAACCCTGTCACAAAGGAGCTGATATTTGCAAAATACATTGGGGCACAAAACAGCAGCAATGAATGGATTACTTTTGACCCTTTCAGTGCAGATTTCAATGCTTGGCCAGTCGTGGAAGTCGCTGCTGCTGCACCAGGCCCAAACATACTTCAACTAGCAGGAACGGGAGAACCTTCTGAAATGCGTGCTGAACAGGAAGAGGATGAGGCCAGAATTGCAAGGTTGGTGCATGAAGCAAGATTGAAGAACCCAATACAAACTATGGAGATTCCTGTTCTTATGCAATAACAAAATACTCAGATTGATGCCAGCTTAATTAACCCTCAACCTATGGGGCAGGTAAGGATGACTACTGTGCTGCCACTCAAAATAAGCACAAAAGATGATGGTTTAAAACAGCTGAAGCGAAGCCGGGTTGATGATTCAGGTGCTGAAATGGAAGTCTCTGATGTTGATGCTGTTCAGACAAACATAGGTTCTCTAGCAATAGATAGTCAGGAAATTGTAATGAGAGATGACTGTCTGTTTGAAAGCGATAATGTAATGGCGGAGCCTGGTCACCAGGCCCGCCAAGGAAAATGAGTTTTATTGTATGGAACTGTCGGGGCCTGGGCAGCCCGAGCACAGTTCCCACTCTGAAATACCTTGTCCGAACATACGAACCGGAAGGAATATTTCTGTCTGAAACAATGGCAGCTATAAATAAAATTGAAGAATTAAAGTATATTTTGTCTTTCGATTCTTGTTTTACGGTTGATAGAATTGGTAGGGGAGGAGGCTTGGCTTTTCTTTGGAAAAATTCTGCTAACTGTACTATTACAAATTTCTCTCAAAATCACATTGATGTTGAAGTGGATGATTTGTTGATTGGGAAATAGCGATTGACTGGTTTTTATGGGATGCCGGAAAATGGAAGAAGAAAAGAATCGTGGGCTTTCTTAAAAAATCTAACAGGAACTTCTCCTTTACCATGGTGCGTTATCGGAGATTTCAACGACATATTGTCATCTGAAGAGAAGAAAGGTAGGTCAGAGAGAGCGCCTTGGCTGATCCGTGGTTTCAGGCAAGCAGTGTTGGACGCGGGTCTGTTTGACTTACACATGACTGGTTATGCTTTCACTTGGTTCAAAAGTCTCGGTACCGCCAGAGCTGTTGAAGAGAAGCTAGATAGAGCACTTGTAAATTCTGATTGGTGTAACATGTTCCCAAATGCAGGTGTTGAATGCCTTTCGACGACATCTTCTGATCATTACCCACTGTGGCTCACTTGCAAATCGGTTACTGCTGTCAACAGAAATCCAAAACGCTTTAAGTTTGAAAATGCGTGGCTCGCTGAACCTGATTTTAAGCAACAAGTGCAGCAACGCTGGAAGCTCTATCCTGAAGAAGGTATCACTCAAAAATTGAGTTATTGCGCCGAAGACCTGATTGATTGGAGTCGTAATAACAACAATTTTTGCCGTGACATCAGTAAAGTTCAAAAGAAAATAG
Protein sequence

>MS.gene81712.t1

MDIWIQAHNLPFGFMAESMGHLLGNHVGKLLKYDFNNNYGTWRKYMRLRVTMNVNEPLKQCLEFEREGADPVTVVFKYENLGNFCYICGLLGHTDAFCPKRQETGAQRQKGGERPACSKIHSRVGKVKIGRNPVTKELIFAKYIGAQNSSNEWITFDPFSADFNAWPVVEVAAAAPGPNILQLAGTGEPSEMRAEQEEDEARIASLINPQPMGQVRMTTVLPLKISTKDDGLKQLKRSRVDDSGAEMEVSDVDAVQTNIGVECLSTTSSDHYPLWLTCKSVTAVNRNPKRFKFENAWLAEPDFKQQVQQRWKLYPEEVKFKRK