AlfalfaGEDB | MS.gene055546

M. sativa cultivar XinJiangDaYe / MS.gene055546

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055546.t1	AES70414.1	53.8	346	93	4	1	345	1	280	3.00E-89	339

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055546.t1	O04244	27.7	137	93	3	83	216	6	139	3.4e-09	64.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055546.t1	G7J017	53.8	346	93	4	1	345	1	280	2.1e-89	339.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene28545	MS.gene055546	PPI
MS.gene28718	MS.gene055546	PPI
MS.gene055546	MS.gene032299	PPI
MS.gene055560	MS.gene055546	PPI
MS.gene29321	MS.gene055546	PPI
MS.gene34618	MS.gene055546	PPI
MS.gene055546	MS.gene08606	PPI
MS.gene038757	MS.gene055546	PPI
MS.gene064966	MS.gene055546	PPI
MS.gene055546	MS.gene000640	PPI
MS.gene016632	MS.gene055546	PPI
MS.gene055546	MS.gene98756	PPI
MS.gene002590	MS.gene055546	PPI
MS.gene055546	MS.gene74639	PPI
MS.gene055546	MS.gene071133	PPI
MS.gene055546	MS.gene60266	PPI
MS.gene055546	MS.gene62249	PPI
MS.gene40828	MS.gene055546	PPI
MS.gene43924	MS.gene055546	PPI
MS.gene046089	MS.gene055546	PPI
MS.gene055546	MS.gene54051	PPI
MS.gene047954	MS.gene055546	PPI
MS.gene77990	MS.gene055546	PPI
MS.gene036423	MS.gene055546	PPI
MS.gene055546	MS.gene37955	PPI
MS.gene054652	MS.gene055546	PPI
MS.gene055546	MS.gene92517	PPI
MS.gene013644	MS.gene055546	PPI
MS.gene055546	MS.gene010890	PPI
MS.gene055546	MS.gene84790	PPI
MS.gene055546	MS.gene044729	PPI
MS.gene72520	MS.gene055546	PPI
MS.gene66651	MS.gene055546	PPI
MS.gene055546	MS.gene60390	PPI
MS.gene055546	MS.gene039692	PPI
MS.gene35202	MS.gene055546	PPI
MS.gene051449	MS.gene055546	PPI
MS.gene019001	MS.gene055546	PPI
MS.gene63743	MS.gene055546	PPI
MS.gene63150	MS.gene055546	PPI
MS.gene48477	MS.gene055546	PPI
MS.gene055546	MS.gene77990	PPI
MS.gene045875	MS.gene055546	PPI
MS.gene055546	MS.gene062511	PPI
MS.gene54051	MS.gene055546	PPI
MS.gene038394	MS.gene055546	PPI
MS.gene30136	MS.gene055546	PPI
MS.gene008861	MS.gene055546	PPI
MS.gene024405	MS.gene055546	PPI
MS.gene055546	MS.gene024841	PPI
MS.gene73261	MS.gene055546	PPI
MS.gene012748	MS.gene055546	PPI
MS.gene024297	MS.gene055546	PPI
MS.gene83979	MS.gene055546	PPI
MS.gene45083	MS.gene055546	PPI
MS.gene92517	MS.gene055546	PPI
MS.gene055546	MS.gene46353	PPI
MS.gene85295	MS.gene055546	PPI
MS.gene057934	MS.gene055546	PPI
MS.gene47792	MS.gene055546	PPI
MS.gene46948	MS.gene055546	PPI
MS.gene98471	MS.gene055546	PPI
MS.gene028714	MS.gene055546	PPI
MS.gene049610	MS.gene055546	PPI
MS.gene055546	MS.gene61651	PPI
MS.gene060718	MS.gene055546	PPI
MS.gene025401	MS.gene055546	PPI
MS.gene93396	MS.gene055546	PPI
MS.gene05576	MS.gene055546	PPI
MS.gene055546	MS.gene09023	PPI
MS.gene055546	MS.gene054508	PPI
MS.gene055546	MS.gene025987	PPI
MS.gene52349	MS.gene055546	PPI
MS.gene05231	MS.gene055546	PPI
MS.gene055546	MS.gene95960	PPI
MS.gene055546	MS.gene043140	PPI
MS.gene98467	MS.gene055546	PPI
MS.gene46353	MS.gene055546	PPI
MS.gene010656	MS.gene055546	PPI
MS.gene42010	MS.gene055546	PPI
MS.gene055546	MS.gene03149	PPI
MS.gene40469	MS.gene055546	PPI
MS.gene041264	MS.gene055546	PPI
MS.gene41979	MS.gene055546	PPI
MS.gene024522	MS.gene055546	PPI
MS.gene055546	MS.gene29321	PPI
MS.gene64130	MS.gene055546	PPI
MS.gene05319	MS.gene055546	PPI
MS.gene050679	MS.gene055546	PPI
MS.gene055546	MS.gene025401	PPI
MS.gene055546	MS.gene015093	PPI
MS.gene20246	MS.gene055546	PPI
MS.gene055223	MS.gene055546	PPI
MS.gene016719	MS.gene055546	PPI
MS.gene055546	MS.gene24278	PPI
MS.gene055546	MS.gene000379	PPI
MS.gene010290	MS.gene055546	PPI
MS.gene055546	MS.gene34618	PPI
MS.gene053747	MS.gene055546	PPI
MS.gene055546	MS.gene59460	PPI
MS.gene65471	MS.gene055546	PPI
MS.gene061850	MS.gene055546	PPI
MS.gene055546	MS.gene98127	PPI
MS.gene055546	MS.gene08713	PPI
MS.gene26433	MS.gene055546	PPI
MS.gene34463	MS.gene055546	PPI
MS.gene048088	MS.gene055546	PPI
MS.gene055546	MS.gene09517	PPI
MS.gene22730	MS.gene055546	PPI
MS.gene003264	MS.gene055546	PPI
MS.gene073582	MS.gene055546	PPI
MS.gene042559	MS.gene055546	PPI
MS.gene058889	MS.gene055546	PPI
MS.gene98616	MS.gene055546	PPI
MS.gene53904	MS.gene055546	PPI
MS.gene08708	MS.gene055546	PPI
MS.gene69586	MS.gene055546	PPI
MS.gene042027	MS.gene055546	PPI
MS.gene85308	MS.gene055546	PPI
MS.gene69490	MS.gene055546	PPI
MS.gene015248	MS.gene055546	PPI
MS.gene055546	MS.gene44144	PPI
MS.gene012889	MS.gene055546	PPI
MS.gene80362	MS.gene055546	PPI
MS.gene44083	MS.gene055546	PPI
MS.gene013193	MS.gene055546	PPI
MS.gene04970	MS.gene055546	PPI
MS.gene08106	MS.gene055546	PPI
MS.gene51799	MS.gene055546	PPI
MS.gene58419	MS.gene055546	PPI
MS.gene96163	MS.gene055546	PPI
MS.gene08713	MS.gene055546	PPI
MS.gene23521	MS.gene055546	PPI
MS.gene29406	MS.gene055546	PPI
MS.gene86275	MS.gene055546	PPI
MS.gene98127	MS.gene055546	PPI
MS.gene055546	MS.gene057934	PPI
MS.gene87529	MS.gene055546	PPI
MS.gene055546	MS.gene37537	PPI
MS.gene04134	MS.gene055546	PPI
MS.gene055546	MS.gene069786	PPI
MS.gene062067	MS.gene055546	PPI
MS.gene055546	MS.gene025158	PPI
MS.gene055546	MS.gene27329	PPI
MS.gene57044	MS.gene055546	PPI
MS.gene055546	MS.gene41393	PPI
MS.gene054508	MS.gene055546	PPI
MS.gene89815	MS.gene055546	PPI
MS.gene017586	MS.gene055546	PPI
MS.gene038676	MS.gene055546	PPI
MS.gene055546	MS.gene016632	PPI
MS.gene055546	MS.gene018474	PPI
MS.gene98756	MS.gene055546	PPI
MS.gene71637	MS.gene055546	PPI
MS.gene042651	MS.gene055546	PPI
MS.gene055546	MS.gene96163	PPI
MS.gene037049	MS.gene055546	PPI
MS.gene034643	MS.gene055546	PPI
MS.gene044729	MS.gene055546	PPI
MS.gene037634	MS.gene055546	PPI
MS.gene24070	MS.gene055546	PPI
MS.gene71588	MS.gene055546	PPI
MS.gene04749	MS.gene055546	PPI
MS.gene055546	MS.gene61499	PPI
MS.gene071784	MS.gene055546	PPI
MS.gene027169	MS.gene055546	PPI
MS.gene055546	MS.gene81194	PPI
MS.gene061579	MS.gene055546	PPI
MS.gene055546	MS.gene060773	PPI
MS.gene039692	MS.gene055546	PPI
MS.gene76420	MS.gene055546	PPI
MS.gene071320	MS.gene055546	PPI
MS.gene42903	MS.gene055546	PPI
MS.gene81712	MS.gene055546	PPI
MS.gene055546	MS.gene038394	PPI
MS.gene026680	MS.gene055546	PPI
MS.gene021555	MS.gene055546	PPI
MS.gene048747	MS.gene055546	PPI
MS.gene055546	MS.gene24070	PPI
MS.gene22200	MS.gene055546	PPI
MS.gene019165	MS.gene055546	PPI
MS.gene26038	MS.gene055546	PPI
MS.gene49057	MS.gene055546	PPI
MS.gene073573	MS.gene055546	PPI
MS.gene031967	MS.gene055546	PPI
MS.gene053373	MS.gene055546	PPI
MS.gene34812	MS.gene055546	PPI
MS.gene02896	MS.gene055546	PPI
MS.gene063214	MS.gene055546	PPI
MS.gene55682	MS.gene055546	PPI
MS.gene97410	MS.gene055546	PPI
MS.gene32415	MS.gene055546	PPI
MS.gene009213	MS.gene055546	PPI
MS.gene060788	MS.gene055546	PPI
MS.gene035626	MS.gene055546	PPI
MS.gene055546	MS.gene068629	PPI
MS.gene055546	MS.gene009781	PPI
MS.gene067340	MS.gene055546	PPI
MS.gene65623	MS.gene055546	PPI
MS.gene27329	MS.gene055546	PPI
MS.gene71675	MS.gene055546	PPI
MS.gene019177	MS.gene055546	PPI
MS.gene69495	MS.gene055546	PPI
MS.gene049512	MS.gene055546	PPI
MS.gene055546	MS.gene21066	PPI
MS.gene038623	MS.gene055546	PPI
MS.gene55209	MS.gene055546	PPI
MS.gene05357	MS.gene055546	PPI
MS.gene055546	MS.gene003264	PPI
MS.gene032311	MS.gene055546	PPI
MS.gene64178	MS.gene055546	PPI
MS.gene004417	MS.gene055546	PPI
MS.gene030090	MS.gene055546	PPI
MS.gene96174	MS.gene055546	PPI
MS.gene018196	MS.gene055546	PPI
MS.gene86849	MS.gene055546	PPI
MS.gene50392	MS.gene055546	PPI
MS.gene072852	MS.gene055546	PPI
MS.gene57019	MS.gene055546	PPI
MS.gene055546	MS.gene49057	PPI
MS.gene41393	MS.gene055546	PPI
MS.gene39956	MS.gene055546	PPI
MS.gene055546	MS.gene048088	PPI
MS.gene055546	MS.gene86868	PPI
MS.gene021968	MS.gene055546	PPI
MS.gene09589	MS.gene055546	PPI
MS.gene43393	MS.gene055546	PPI
MS.gene057828	MS.gene055546	PPI
MS.gene34197	MS.gene055546	PPI
MS.gene008275	MS.gene055546	PPI
MS.gene66650	MS.gene055546	PPI
MS.gene005120	MS.gene055546	PPI
MS.gene055546	MS.gene012889	PPI
MS.gene048072	MS.gene055546	PPI
MS.gene060386	MS.gene055546	PPI
MS.gene05436	MS.gene055546	PPI
MS.gene059736	MS.gene055546	PPI
MS.gene70932	MS.gene055546	PPI
MS.gene35175	MS.gene055546	PPI
MS.gene81194	MS.gene055546	PPI
MS.gene025675	MS.gene055546	PPI
MS.gene069786	MS.gene055546	PPI
MS.gene021965	MS.gene055546	PPI
MS.gene055683	MS.gene055546	PPI
MS.gene042614	MS.gene055546	PPI
MS.gene41643	MS.gene055546	PPI
MS.gene013890	MS.gene055546	PPI
MS.gene26844	MS.gene055546	PPI
MS.gene060773	MS.gene055546	PPI
MS.gene021647	MS.gene055546	PPI
MS.gene96109	MS.gene055546	PPI
MS.gene69584	MS.gene055546	PPI
MS.gene048706	MS.gene055546	PPI
MS.gene055546	MS.gene060788	PPI
MS.gene009121	MS.gene055546	PPI
MS.gene94654	MS.gene055546	PPI
MS.gene024841	MS.gene055546	PPI
MS.gene51804	MS.gene055546	PPI
MS.gene87845	MS.gene055546	PPI
MS.gene36069	MS.gene055546	PPI
MS.gene055546	MS.gene027994	PPI
MS.gene055546	MS.gene022929	PPI
MS.gene95960	MS.gene055546	PPI
MS.gene055546	MS.gene007022	PPI
MS.gene61452	MS.gene055546	PPI
MS.gene83772	MS.gene055546	PPI
MS.gene055546	MS.gene69584	PPI
MS.gene008927	MS.gene055546	PPI
MS.gene016102	MS.gene055546	PPI
MS.gene46941	MS.gene055546	PPI
MS.gene045614	MS.gene055546	PPI
MS.gene96541	MS.gene055546	PPI
MS.gene049561	MS.gene055546	PPI
MS.gene000634	MS.gene055546	PPI
MS.gene009781	MS.gene055546	PPI
MS.gene63762	MS.gene055546	PPI
MS.gene055546	MS.gene048342	PPI
MS.gene055546	MS.gene069624	PPI
MS.gene30208	MS.gene055546	PPI
MS.gene028716	MS.gene055546	PPI
MS.gene025873	MS.gene055546	PPI
MS.gene012019	MS.gene055546	PPI
MS.gene06164	MS.gene055546	PPI
MS.gene013676	MS.gene055546	PPI
MS.gene05358	MS.gene055546	PPI
MS.gene95561	MS.gene055546	PPI
MS.gene074257	MS.gene055546	PPI
MS.gene055546	MS.gene88393	PPI
MS.gene065627	MS.gene055546	PPI
MS.gene055546	MS.gene008658	PPI
MS.gene010890	MS.gene055546	PPI
MS.gene018745	MS.gene055546	PPI
MS.gene000629	MS.gene055546	PPI
MS.gene65546	MS.gene055546	PPI
MS.gene09023	MS.gene055546	PPI
MS.gene059269	MS.gene055546	PPI
MS.gene000506	MS.gene055546	PPI
MS.gene032299	MS.gene055546	PPI
MS.gene03936	MS.gene055546	PPI
MS.gene038098	MS.gene055546	PPI
MS.gene56991	MS.gene055546	PPI
MS.gene055546	MS.gene65474	PPI
MS.gene60059	MS.gene055546	PPI
MS.gene85538	MS.gene055546	PPI
MS.gene062511	MS.gene055546	PPI
MS.gene22111	MS.gene055546	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055546.t1	MTR_3g053920	52.722	349	92	5	1	345	1	280	1.76e-112	330
MS.gene055546.t1	MTR_2g061500	75.969	129	31	0	1	129	1	129	6.68e-69	213
MS.gene055546.t1	MTR_4g103530	73.196	97	26	0	55	151	1	97	1.05e-45	155
MS.gene055546.t1	MTR_7g011710	74.118	85	22	0	55	139	1	85	2.21e-39	138
MS.gene055546.t1	MTR_6g033215	35.838	173	109	1	29	199	122	294	2.76e-36	134
MS.gene055546.t1	MTR_0010s0370	36.571	175	105	5	8	177	4	177	8.62e-30	114
MS.gene055546.t1	MTR_0010s0350	36.571	175	105	5	8	177	4	177	8.62e-30	114
MS.gene055546.t1	MTR_8g463110	56.122	98	35	2	289	385	1	91	1.08e-15	79.3
MS.gene055546.t1	MTR_1g036720	31.731	104	69	1	11	112	7	110	5.39e-13	65.5
MS.gene055546.t1	MTR_4g020545	34.483	116	60	3	63	178	79	178	9.18e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055546.t1	AT2G17920	25.701	214	153	3	7	216	8	219	3.29e-17	82.0
MS.gene055546.t1	AT5G36228	26.904	197	137	3	36	227	39	233	1.78e-16	80.5
MS.gene055546.t1	AT3G31430	28.108	185	121	5	36	215	134	311	2.80e-15	76.6
MS.gene055546.t1	AT2G41590	28.472	144	95	3	77	216	80	219	1.02e-14	75.5
MS.gene055546.t1	AT2G13450	26.776	183	128	3	36	215	39	218	1.84e-14	74.7
MS.gene055546.t1	AT3G57587	26.056	142	101	2	77	216	80	219	3.06e-14	73.9
MS.gene055546.t1	AT3G47920	25.352	142	102	2	77	216	9	148	5.18e-12	66.6

Find 103 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTAATTCATTTGGATATC+TGG	0.113881	3.2:+81940264	MS.gene055546:CDS
CTGCTTGATCGGTCGTGTTT+TGG	0.188329	3.2:+81940019	MS.gene055546:CDS
CGGACGATGGAGTACGTAAT+TGG	0.209232	3.2:+81940585	MS.gene055546:CDS
CACTGTTCCTCTAATTCATT+TGG	0.259561	3.2:+81940256	MS.gene055546:CDS
GATATCTGGGTGCAGGTCTA+TGG	0.275435	3.2:+81940278	MS.gene055546:CDS
CAGATTCAGCAGATAGGTTA+AGG	0.317540	3.2:-81939974	None:intergenic
TTTACTGCAGGGTCGTTCTA+TGG	0.328442	3.2:+81940928	MS.gene055546:CDS
AAACCTGTTACGACTCGTAT+AGG	0.344071	3.2:+81940620	MS.gene055546:CDS
CCTATATGCGCCTCAAAGTC+TGG	0.351054	3.2:+81940399	MS.gene055546:CDS
CAGGGTCGTTCTATGGTACT+TGG	0.351644	3.2:+81940935	MS.gene055546:CDS
CAAGGCATTGGCAGCTTTCT+CGG	0.355349	3.2:+81940335	MS.gene055546:CDS
TGTCACTGTACCGCTGAAAA+AGG	0.360408	3.2:+81940427	MS.gene055546:CDS
GGACGATGGAGTACGTAATT+GGG	0.361200	3.2:+81940586	MS.gene055546:CDS
CTTTGATGCGGCACAACATC+AGG	0.362158	3.2:-81940680	None:intergenic
CAGGTCTATGGATTGCCCTT+TGG	0.363802	3.2:+81940290	MS.gene055546:CDS
GACCCTATACGAGTCGTAAC+AGG	0.367774	3.2:-81940623	None:intergenic
CAGATATCCAAATGAATTAG+AGG	0.368589	3.2:-81940263	None:intergenic
TGCTACAAACAGATGGCTTC+AGG	0.376139	3.2:+81940649	MS.gene055546:CDS
TCTAATTCATTTGGATATCT+GGG	0.377178	3.2:+81940265	MS.gene055546:CDS
AATGGTGTTGCGACCATCAT+AGG	0.380913	3.2:-81940369	None:intergenic
TGCTATTAATGCTATTAAAC+AGG	0.387604	3.2:+81940799	MS.gene055546:CDS
TGTTACAGGTGTGGCATTCT+CGG	0.402641	3.2:+81940521	MS.gene055546:CDS
CATCAAGCTTGTGGTGGAAC+TGG	0.403105	3.2:-81940148	None:intergenic
AACCTGTTACGACTCGTATA+GGG	0.403226	3.2:+81940621	MS.gene055546:CDS
GTGTCCCGACTTGTTTGAAT+TGG	0.427535	3.2:+81940559	MS.gene055546:CDS
CTCGGCCTCAAATTTACTGC+AGG	0.434485	3.2:+81940916	MS.gene055546:CDS
GGTGCGGCCAGCAGAAGAAC+TGG	0.435350	3.2:-81940714	None:intergenic
AGGTTTCAGATTCAGCAGAT+AGG	0.439470	3.2:-81939980	None:intergenic
AGATTCAGCAGATAGGTTAA+GGG	0.441257	3.2:-81939973	None:intergenic
GAGGCCGAGACTCCTGTAGA+TGG	0.454282	3.2:-81940902	None:intergenic
ATGTCCCGTGCGTGGAAACC+TGG	0.455231	3.2:+81940080	MS.gene055546:CDS
GACCGATCAAGCAGTGATCT+AGG	0.458613	3.2:-81940010	None:intergenic
CTTTGGTTTCATTCAATCAA+AGG	0.459068	3.2:+81940307	MS.gene055546:CDS
GGAGGACTTTCCATTCTAAA+GGG	0.459478	3.2:-81941068	None:intergenic
CCAGACTTTGAGGCGCATAT+AGG	0.464440	3.2:-81940399	None:intergenic
GTGTGGCATTCTCGGACATT+CGG	0.466234	3.2:+81940529	MS.gene055546:CDS
ATAATAGTTACGCGTTTGCC+AGG	0.478326	3.2:-81940098	None:intergenic
GCAAAAGTGAAAGGAGCAGC+AGG	0.480319	3.2:-81940962	None:intergenic
GTATCCTTGATGAGGGTCCT+TGG	0.481984	3.2:+81940177	MS.gene055546:CDS
CGACTTGTTTGAATTGGACT+CGG	0.483849	3.2:+81940565	MS.gene055546:CDS
TCATTTGGATATCTGGGTGC+AGG	0.490055	3.2:+81940271	MS.gene055546:CDS
GTTTGCCAGGTTTCCACGCA+CGG	0.490364	3.2:-81940085	None:intergenic
TTCTCCATCTACAGGAGTCT+CGG	0.493364	3.2:+81940898	MS.gene055546:CDS
ATGCTTCCTTCTCCATCTAC+AGG	0.496559	3.2:+81940890	MS.gene055546:CDS
TTCCAAGCCAGTTCTTCTGC+TGG	0.498549	3.2:+81940707	MS.gene055546:CDS
GGTTTCATTCAATCAAAGGT+TGG	0.507457	3.2:+81940311	MS.gene055546:CDS
TGTTGCGACCATCATAGGTT+AGG	0.510688	3.2:-81940364	None:intergenic
GTCATGGAACGCATTGCTCC+TGG	0.512312	3.2:+81940221	MS.gene055546:CDS
TCAAAGGTTGGTCAAGGCAT+TGG	0.512470	3.2:+81940323	MS.gene055546:CDS
TTTGATTGAATGAAACCAAA+GGG	0.513269	3.2:-81940305	None:intergenic
TTTGGACGATCGTGAAATCA+AGG	0.514839	3.2:+81940037	MS.gene055546:CDS
TTTGAATTGGACTCGGACGA+TGG	0.520707	3.2:+81940572	MS.gene055546:CDS
ACGACCCTGCAGTAAATTTG+AGG	0.521272	3.2:-81940921	None:intergenic
TGACATCAATCCAGACTTTG+AGG	0.530919	3.2:-81940409	None:intergenic
CAACATTGACGGTCTTACCT+TGG	0.534894	3.2:+81939935	MS.gene055546:CDS
ATTCAATCAAAGGTTGGTCA+AGG	0.545909	3.2:+81940317	MS.gene055546:CDS
GAGTCCAATTCAAACAAGTC+GGG	0.547426	3.2:-81940563	None:intergenic
GTTGAGTTGTGTGGGATCCA+TGG	0.548620	3.2:-81939916	None:intergenic
TGAATTAGAGGAACAGTGAT+CGG	0.549035	3.2:-81940251	None:intergenic
CCCACACAACTCAACATTGA+CGG	0.549913	3.2:+81939924	MS.gene055546:CDS
TCAAAACAGAATTGTCAGCA+TGG	0.555985	3.2:-81940736	None:intergenic
ATGCGGCACAACATCAGGGA+TGG	0.556865	3.2:-81940675	None:intergenic
ATCCTAGATCACTGCTTGAT+CGG	0.559464	3.2:+81940008	MS.gene055546:CDS
TTTGTACCAGCAAAAGTGAA+AGG	0.560500	3.2:-81940971	None:intergenic
CAGAATTGTCAGCATGGGTG+CGG	0.561489	3.2:-81940730	None:intergenic
GGTACTCTCCTAACCTATGA+TGG	0.562210	3.2:+81940356	MS.gene055546:CDS
GGCCAGCAGAAGAACTGGCT+TGG	0.565580	3.2:-81940709	None:intergenic
TGCCGCTCGTATCCTTGATG+AGG	0.569722	3.2:+81940169	MS.gene055546:CDS
CAAAACAGAATTGTCAGCAT+GGG	0.573225	3.2:-81940735	None:intergenic
ACAGTGATCGGAACCACACC+AGG	0.573710	3.2:-81940239	None:intergenic
TTTGCCAGGTTTCCACGCAC+GGG	0.575795	3.2:-81940084	None:intergenic
GGTCTTACCTTGGCTGACGA+AGG	0.583933	3.2:+81939945	MS.gene055546:CDS
GCCGCTCGTATCCTTGATGA+GGG	0.585959	3.2:+81940170	MS.gene055546:CDS
GGACATTCGTTCACTGAAGT+AGG	0.587017	3.2:-81940063	None:intergenic
GGTCAAACCTTCGTCAGCCA+AGG	0.587526	3.2:-81939952	None:intergenic
TACGAGCGGCATCAAGCTTG+TGG	0.589023	3.2:-81940157	None:intergenic
CAGTGTCTACAGCAAGCATA+CGG	0.590441	3.2:-81940778	None:intergenic
TGCGGCACAACATCAGGGAT+GGG	0.590978	3.2:-81940674	None:intergenic
CATTCTCGGACATTCGGACA+AGG	0.591084	3.2:+81940535	MS.gene055546:CDS
TGATAACTTTCATATAGTCA+TGG	0.592825	3.2:+81940205	MS.gene055546:CDS
GTGAACGAATGTCCCGTGCG+TGG	0.593922	3.2:+81940072	MS.gene055546:CDS
GCTTGGAAGTTACTTTGATG+CGG	0.594151	3.2:-81940692	None:intergenic
GAGACTCCTGTAGATGGAGA+AGG	0.602523	3.2:-81940896	None:intergenic
ACCGTCAATGTTGAGTTGTG+TGG	0.602644	3.2:-81939925	None:intergenic
TGGAAAGTTCGTGCTAGCAA+TGG	0.606670	3.2:+81940452	MS.gene055546:CDS
CTTTGATTGAATGAAACCAA+AGG	0.608719	3.2:-81940306	None:intergenic
CGAGTCCAATTCAAACAAGT+CGG	0.610252	3.2:-81940564	None:intergenic
GAGCGGCATCAAGCTTGTGG+TGG	0.615991	3.2:-81940154	None:intergenic
GACGATGGAGTACGTAATTG+GGG	0.621097	3.2:+81940587	MS.gene055546:CDS
GGTCGGCTGCTACAAACAGA+TGG	0.622085	3.2:+81940642	MS.gene055546:CDS
CCGTCAATGTTGAGTTGTGT+GGG	0.623706	3.2:-81939924	None:intergenic
ATAACCAAGGACCCTCATCA+AGG	0.624768	3.2:-81940181	None:intergenic
GCGCATATAGGAGCTATGAA+TGG	0.625349	3.2:-81940387	None:intergenic
TTTGATGCGGCACAACATCA+GGG	0.625918	3.2:-81940679	None:intergenic
GTAGATGGAGAAGGAAGCAT+CGG	0.627617	3.2:-81940887	None:intergenic
ATCAAGCAGTGATCTAGGAT+AGG	0.632523	3.2:-81940005	None:intergenic
TCGGCCTCAAATTTACTGCA+GGG	0.638651	3.2:+81940917	MS.gene055546:CDS
GGAACGCATTGCTCCTGGTG+TGG	0.640570	3.2:+81940226	MS.gene055546:CDS
GCAGAGCAGTCTGAGCAGCG+AGG	0.684222	3.2:-81940826	None:intergenic
ACCCTCATCAAGGATACGAG+CGG	0.685761	3.2:-81940171	None:intergenic
TGAAAGTTATCATATAACCA+AGG	0.688479	3.2:-81940194	None:intergenic
GCAGTGATCTAGGATAGGAG+AGG	0.689001	3.2:-81940000	None:intergenic
TGTTACGACTCGTATAGGGT+CGG	0.713178	3.2:+81940625	MS.gene055546:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAAAAAAATAGTAACAA+AGG	-	chr3.2:81940480-81940499	None:intergenic	15.0%
!!!	TTTTTCAAATATGAGAAACT+GGG	+	chr3.2:81940491-81940510	MS.gene055546:CDS	20.0%
!!!	TTTTTTCAAATATGAGAAAC+TGG	+	chr3.2:81940490-81940509	MS.gene055546:CDS	20.0%
!	TCTAATTCATTTGGATATCT+GGG	+	chr3.2:81940265-81940284	MS.gene055546:CDS	25.0%
!	TGAAAGTTATCATATAACCA+AGG	-	chr3.2:81940197-81940216	None:intergenic	25.0%
!	TGATAACTTTCATATAGTCA+TGG	+	chr3.2:81940205-81940224	MS.gene055546:CDS	25.0%
!	TGCTATTAATGCTATTAAAC+AGG	+	chr3.2:81940799-81940818	MS.gene055546:CDS	25.0%
!	TTTGATTGAATGAAACCAAA+GGG	-	chr3.2:81940308-81940327	None:intergenic	25.0%
	CAAGCTAAAAATGTCACAAA+TGG	+	chr3.2:81940848-81940867	MS.gene055546:CDS	30.0%
	CAGATATCCAAATGAATTAG+AGG	-	chr3.2:81940266-81940285	None:intergenic	30.0%
	CTCTAATTCATTTGGATATC+TGG	+	chr3.2:81940264-81940283	MS.gene055546:CDS	30.0%
	CTTTGATTGAATGAAACCAA+AGG	-	chr3.2:81940309-81940328	None:intergenic	30.0%
!	CTTTGGTTTCATTCAATCAA+AGG	+	chr3.2:81940307-81940326	MS.gene055546:CDS	30.0%
	AAAAATGTCACAAATGGTGC+TGG	+	chr3.2:81940854-81940873	MS.gene055546:CDS	35.0%
	AGATTCAGCAGATAGGTTAA+GGG	-	chr3.2:81939976-81939995	None:intergenic	35.0%
	CAAAACAGAATTGTCAGCAT+GGG	-	chr3.2:81940738-81940757	None:intergenic	35.0%
	CACTGTTCCTCTAATTCATT+TGG	+	chr3.2:81940256-81940275	MS.gene055546:CDS	35.0%
	GGTTTCATTCAATCAAAGGT+TGG	+	chr3.2:81940311-81940330	MS.gene055546:CDS	35.0%
	TCAAAACAGAATTGTCAGCA+TGG	-	chr3.2:81940739-81940758	None:intergenic	35.0%
	TGAATTAGAGGAACAGTGAT+CGG	-	chr3.2:81940254-81940273	None:intergenic	35.0%
	TTTGTACCAGCAAAAGTGAA+AGG	-	chr3.2:81940974-81940993	None:intergenic	35.0%
!	ATTCAATCAAAGGTTGGTCA+AGG	+	chr3.2:81940317-81940336	MS.gene055546:CDS	35.0%
!!!	AACTGGGTGTTTTTTGTTAC+AGG	+	chr3.2:81940507-81940526	MS.gene055546:CDS	35.0%
	AAACCTGTTACGACTCGTAT+AGG	+	chr3.2:81940620-81940639	MS.gene055546:CDS	40.0%
	AACCTGTTACGACTCGTATA+GGG	+	chr3.2:81940621-81940640	MS.gene055546:CDS	40.0%
	AGGTTTCAGATTCAGCAGAT+AGG	-	chr3.2:81939983-81940002	None:intergenic	40.0%
	ATAATAGTTACGCGTTTGCC+AGG	-	chr3.2:81940101-81940120	None:intergenic	40.0%
	ATCCTAGATCACTGCTTGAT+CGG	+	chr3.2:81940008-81940027	MS.gene055546:CDS	40.0%
	CAGATTCAGCAGATAGGTTA+AGG	-	chr3.2:81939977-81939996	None:intergenic	40.0%
	CGAGTCCAATTCAAACAAGT+CGG	-	chr3.2:81940567-81940586	None:intergenic	40.0%
	GAGTCCAATTCAAACAAGTC+GGG	-	chr3.2:81940566-81940585	None:intergenic	40.0%
	TGACATCAATCCAGACTTTG+AGG	-	chr3.2:81940412-81940431	None:intergenic	40.0%
	TTTGGACGATCGTGAAATCA+AGG	+	chr3.2:81940037-81940056	MS.gene055546:CDS	40.0%
!	ATCAAGCAGTGATCTAGGAT+AGG	-	chr3.2:81940008-81940027	None:intergenic	40.0%
!	CGACTTGTTTGAATTGGACT+CGG	+	chr3.2:81940565-81940584	MS.gene055546:CDS	40.0%
!	GCTTGGAAGTTACTTTGATG+CGG	-	chr3.2:81940695-81940714	None:intergenic	40.0%
!!!	ACTTTCCACTCCTTTTTCAG+CGG	-	chr3.2:81940440-81940459	None:intergenic	40.0%
!!!	CGACAACGTTGTTTTTCTTG+AGG	+	chr3.2:81941006-81941025	MS.gene055546:CDS	40.0%
!!!	GGTGTTTTTTGTTACAGGTG+TGG	+	chr3.2:81940512-81940531	MS.gene055546:CDS	40.0%
!!!	GTTGTTTTTCTTGAGGATGC+TGG	+	chr3.2:81941013-81941032	MS.gene055546:CDS	40.0%
	ACGACCCTGCAGTAAATTTG+AGG	-	chr3.2:81940924-81940943	None:intergenic	45.0%
	ATAACCAAGGACCCTCATCA+AGG	-	chr3.2:81940184-81940203	None:intergenic	45.0%
	ATGCTTCCTTCTCCATCTAC+AGG	+	chr3.2:81940890-81940909	MS.gene055546:CDS	45.0%
	CAACATTGACGGTCTTACCT+TGG	+	chr3.2:81939935-81939954	MS.gene055546:CDS	45.0%
	CAGTGTCTACAGCAAGCATA+CGG	-	chr3.2:81940781-81940800	None:intergenic	45.0%
	CCCACACAACTCAACATTGA+CGG	+	chr3.2:81939924-81939943	MS.gene055546:CDS	45.0%
	GACGATGGAGTACGTAATTG+GGG	+	chr3.2:81940587-81940606	MS.gene055546:CDS	45.0%
	GCGCATATAGGAGCTATGAA+TGG	-	chr3.2:81940390-81940409	None:intergenic	45.0%
	GGACATTCGTTCACTGAAGT+AGG	-	chr3.2:81940066-81940085	None:intergenic	45.0%
	GGACGATGGAGTACGTAATT+GGG	+	chr3.2:81940586-81940605	MS.gene055546:CDS	45.0%
	GGTACTCTCCTAACCTATGA+TGG	+	chr3.2:81940356-81940375	MS.gene055546:CDS	45.0%
	TCATTTGGATATCTGGGTGC+AGG	+	chr3.2:81940271-81940290	MS.gene055546:CDS	45.0%
	TCGGCCTCAAATTTACTGCA+GGG	+	chr3.2:81940917-81940936	MS.gene055546:CDS	45.0%
	TGCTACAAACAGATGGCTTC+AGG	+	chr3.2:81940649-81940668	MS.gene055546:CDS	45.0%
	TGGAAAGTTCGTGCTAGCAA+TGG	+	chr3.2:81940452-81940471	MS.gene055546:CDS	45.0%
	TGTCACTGTACCGCTGAAAA+AGG	+	chr3.2:81940427-81940446	MS.gene055546:CDS	45.0%
	TGTTACAGGTGTGGCATTCT+CGG	+	chr3.2:81940521-81940540	MS.gene055546:CDS	45.0%
	TGTTACGACTCGTATAGGGT+CGG	+	chr3.2:81940625-81940644	MS.gene055546:CDS	45.0%
	TGTTGCGACCATCATAGGTT+AGG	-	chr3.2:81940367-81940386	None:intergenic	45.0%
	TTCTCCATCTACAGGAGTCT+CGG	+	chr3.2:81940898-81940917	MS.gene055546:CDS	45.0%
	TTTACTGCAGGGTCGTTCTA+TGG	+	chr3.2:81940928-81940947	MS.gene055546:CDS	45.0%
	TTTGAATTGGACTCGGACGA+TGG	+	chr3.2:81940572-81940591	MS.gene055546:CDS	45.0%
	TTTGATGCGGCACAACATCA+GGG	-	chr3.2:81940682-81940701	None:intergenic	45.0%
!	ACCGTCAATGTTGAGTTGTG+TGG	-	chr3.2:81939928-81939947	None:intergenic	45.0%
!	CCGTCAATGTTGAGTTGTGT+GGG	-	chr3.2:81939927-81939946	None:intergenic	45.0%
!	GTAGATGGAGAAGGAAGCAT+CGG	-	chr3.2:81940890-81940909	None:intergenic	45.0%
!	GTGTCCCGACTTGTTTGAAT+TGG	+	chr3.2:81940559-81940578	MS.gene055546:CDS	45.0%
!	TCAAAGGTTGGTCAAGGCAT+TGG	+	chr3.2:81940323-81940342	MS.gene055546:CDS	45.0%
!!	AATGGTGTTGCGACCATCAT+AGG	-	chr3.2:81940372-81940391	None:intergenic	45.0%
	ACCCTCATCAAGGATACGAG+CGG	-	chr3.2:81940174-81940193	None:intergenic	50.0%
	CAGAATTGTCAGCATGGGTG+CGG	-	chr3.2:81940733-81940752	None:intergenic	50.0%
	CAGGGTCGTTCTATGGTACT+TGG	+	chr3.2:81940935-81940954	MS.gene055546:CDS	50.0%
	CATTCTCGGACATTCGGACA+AGG	+	chr3.2:81940535-81940554	MS.gene055546:CDS	50.0%
	CCTATATGCGCCTCAAAGTC+TGG	+	chr3.2:81940399-81940418	MS.gene055546:CDS	50.0%
	CGGACGATGGAGTACGTAAT+TGG	+	chr3.2:81940585-81940604	MS.gene055546:CDS	50.0%
	CTCGGCCTCAAATTTACTGC+AGG	+	chr3.2:81940916-81940935	MS.gene055546:CDS	50.0%
	CTGTACCGCTGAAAAAGGAG+TGG	+	chr3.2:81940432-81940451	MS.gene055546:CDS	50.0%
	CTTTGATGCGGCACAACATC+AGG	-	chr3.2:81940683-81940702	None:intergenic	50.0%
	GACCCTATACGAGTCGTAAC+AGG	-	chr3.2:81940626-81940645	None:intergenic	50.0%
	GACCGATCAAGCAGTGATCT+AGG	-	chr3.2:81940013-81940032	None:intergenic	50.0%
	GAGACTCCTGTAGATGGAGA+AGG	-	chr3.2:81940899-81940918	None:intergenic	50.0%
	GATATCTGGGTGCAGGTCTA+TGG	+	chr3.2:81940278-81940297	MS.gene055546:CDS	50.0%
	GCAAAAGTGAAAGGAGCAGC+AGG	-	chr3.2:81940965-81940984	None:intergenic	50.0%
	GTATCCTTGATGAGGGTCCT+TGG	+	chr3.2:81940177-81940196	MS.gene055546:CDS	50.0%
	GTGTGGCATTCTCGGACATT+CGG	+	chr3.2:81940529-81940548	MS.gene055546:CDS	50.0%
	TTCCAAGCCAGTTCTTCTGC+TGG	+	chr3.2:81940707-81940726	MS.gene055546:CDS	50.0%
!	CAGGTCTATGGATTGCCCTT+TGG	+	chr3.2:81940290-81940309	MS.gene055546:CDS	50.0%
!	CATCAAGCTTGTGGTGGAAC+TGG	-	chr3.2:81940151-81940170	None:intergenic	50.0%
!	CTGCTTGATCGGTCGTGTTT+TGG	+	chr3.2:81940019-81940038	MS.gene055546:CDS	50.0%
!	GCAGTGATCTAGGATAGGAG+AGG	-	chr3.2:81940003-81940022	None:intergenic	50.0%
!!	CAAGGCATTGGCAGCTTTCT+CGG	+	chr3.2:81940335-81940354	MS.gene055546:CDS	50.0%
!!	CCAGACTTTGAGGCGCATAT+AGG	-	chr3.2:81940402-81940421	None:intergenic	50.0%
!!	GCTGCTCCTTTCACTTTTGC+TGG	+	chr3.2:81940965-81940984	MS.gene055546:CDS	50.0%
	ACAGTGATCGGAACCACACC+AGG	-	chr3.2:81940242-81940261	None:intergenic	55.0%
	ATGCGGCACAACATCAGGGA+TGG	-	chr3.2:81940678-81940697	None:intergenic	55.0%
	GCCGCTCGTATCCTTGATGA+GGG	+	chr3.2:81940170-81940189	MS.gene055546:CDS	55.0%
	GGTCAAACCTTCGTCAGCCA+AGG	-	chr3.2:81939955-81939974	None:intergenic	55.0%
	GGTCGGCTGCTACAAACAGA+TGG	+	chr3.2:81940642-81940661	MS.gene055546:CDS	55.0%
	GGTCTTACCTTGGCTGACGA+AGG	+	chr3.2:81939945-81939964	MS.gene055546:CDS	55.0%
	GTCATGGAACGCATTGCTCC+TGG	+	chr3.2:81940221-81940240	MS.gene055546:CDS	55.0%
	GTTTGCCAGGTTTCCACGCA+CGG	-	chr3.2:81940088-81940107	None:intergenic	55.0%
	TACGAGCGGCATCAAGCTTG+TGG	-	chr3.2:81940160-81940179	None:intergenic	55.0%
	TGCCGCTCGTATCCTTGATG+AGG	+	chr3.2:81940169-81940188	MS.gene055546:CDS	55.0%
	TGCGGCACAACATCAGGGAT+GGG	-	chr3.2:81940677-81940696	None:intergenic	55.0%
	TTTGCCAGGTTTCCACGCAC+GGG	-	chr3.2:81940087-81940106	None:intergenic	55.0%
	ATGTCCCGTGCGTGGAAACC+TGG	+	chr3.2:81940080-81940099	MS.gene055546:CDS	60.0%
	GAGCGGCATCAAGCTTGTGG+TGG	-	chr3.2:81940157-81940176	None:intergenic	60.0%
	GAGGCCGAGACTCCTGTAGA+TGG	-	chr3.2:81940905-81940924	None:intergenic	60.0%
	GGAACGCATTGCTCCTGGTG+TGG	+	chr3.2:81940226-81940245	MS.gene055546:CDS	60.0%
	GGCCAGCAGAAGAACTGGCT+TGG	-	chr3.2:81940712-81940731	None:intergenic	60.0%
	GTGAACGAATGTCCCGTGCG+TGG	+	chr3.2:81940072-81940091	MS.gene055546:CDS	60.0%
	GCAGAGCAGTCTGAGCAGCG+AGG	-	chr3.2:81940829-81940848	None:intergenic	65.0%
	GGTGCGGCCAGCAGAAGAAC+TGG	-	chr3.2:81940717-81940736	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	81939918	81941075	81939918	ID=MS.gene055546
chr3.2	mRNA	81939918	81941075	81939918	ID=MS.gene055546.t1;Parent=MS.gene055546
chr3.2	exon	81939918	81941075	81939918	ID=MS.gene055546.t1.exon1;Parent=MS.gene055546.t1
chr3.2	CDS	81939918	81941075	81939918	ID=cds.MS.gene055546.t1;Parent=MS.gene055546.t1

Gene Sequence

>MS.gene055546

ATGGATCCCACACAACTCAACATTGACGGTCTTACCTTGGCTGACGAAGGTTTGACCCTTAACCTATCTGCTGAATCTGAAACCTCTCCTATCCTAGATCACTGCTTGATCGGTCGTGTTTTGGACGATCGTGAAATCAAGGCAGCCTACTTCAGTGAACGAATGTCCCGTGCGTGGAAACCTGGCAAACGCGTAACTATTATCAAATCTGATGATGATCGCTTCTTGTTCCAGTTCCACCACAAGCTTGATGCCGCTCGTATCCTTGATGAGGGTCCTTGGTTATATGATAACTTTCATATAGTCATGGAACGCATTGCTCCTGGTGTGGTTCCGATCACTGTTCCTCTAATTCATTTGGATATCTGGGTGCAGGTCTATGGATTGCCCTTTGGTTTCATTCAATCAAAGGTTGGTCAAGGCATTGGCAGCTTTCTCGGTACTCTCCTAACCTATGATGGTCGCAACACCATTCATAGCTCCTATATGCGCCTCAAAGTCTGGATTGATGTCACTGTACCGCTGAAAAAGGAGTGGAAAGTTCGTGCTAGCAATGGTTCCTTTGTTACTATTTTTTTCAAATATGAGAAACTGGGTGTTTTTTGTTACAGGTGTGGCATTCTCGGACATTCGGACAAGGTGTGTCCCGACTTGTTTGAATTGGACTCGGACGATGGAGTACGTAATTGGGGACTTGATCTCAAACCTGTTACGACTCGTATAGGGTCGGCTGCTACAAACAGATGGCTTCAGGATCCCATCCCTGATGTTGTGCCGCATCAAAGTAACTTCCAAGCCAGTTCTTCTGCTGGCCGCACCCATGCTGACAATTCTGTTTTGAATTTTAATAACTTTAATAACCGTATGCTTGCTGTAGACACTGCTATTAATGCTATTAAACAGGATGTCCTCGCTGCTCAGACTGCTCTGCAAGCTAAAAATGTCACAAATGGTGCTGGTTGTCAAGTTCCGATGCTTCCTTCTCCATCTACAGGAGTCTCGGCCTCAAATTTACTGCAGGGTCGTTCTATGGTACTTGGTTTGCCTGCTGCTCCTTTCACTTTTGCTGGTACAAATAATACTCAAAGCGACAACGTTGTTTTTCTTGAGGATGCTGGTTCTGACTTGAAGAAACGCAAGTGTATGCTCGCCCTTTAG

Protein sequence

>MS.gene055546.t1

MDPTQLNIDGLTLADEGLTLNLSAESETSPILDHCLIGRVLDDREIKAAYFSERMSRAWKPGKRVTIIKSDDDRFLFQFHHKLDAARILDEGPWLYDNFHIVMERIAPGVVPITVPLIHLDIWVQVYGLPFGFIQSKVGQGIGSFLGTLLTYDGRNTIHSSYMRLKVWIDVTVPLKKEWKVRASNGSFVTIFFKYEKLGVFCYRCGILGHSDKVCPDLFELDSDDGVRNWGLDLKPVTTRIGSAATNRWLQDPIPDVVPHQSNFQASSSAGRTHADNSVLNFNNFNNRMLAVDTAINAIKQDVLAAQTALQAKNVTNGAGCQVPMLPSPSTGVSASNLLQGRSMVLGLPAAPFTFAGTNNTQSDNVVFLEDAGSDLKKRKCMLAL