Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03936.t1 | PNX88800.1 | 53.9 | 219 | 101 | 0 | 1 | 219 | 1 | 219 | 3.80E-65 | 258.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03936.t1 | A0A2K3MDD6 | 53.9 | 219 | 101 | 0 | 1 | 219 | 1 | 219 | 2.8e-65 | 258.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03936.t1 | MTR_3g053920 | 37.423 | 163 | 87 | 1 | 2 | 164 | 5 | 152 | 9.04e-38 | 133 |
| MS.gene03936.t1 | MTR_2g061500 | 41.129 | 124 | 73 | 0 | 3 | 126 | 6 | 129 | 3.42e-33 | 117 |
| MS.gene03936.t1 | MTR_0010s0370 | 32.000 | 175 | 115 | 2 | 4 | 174 | 3 | 177 | 1.41e-29 | 109 |
| MS.gene03936.t1 | MTR_0010s0350 | 32.000 | 175 | 115 | 2 | 4 | 174 | 3 | 177 | 1.41e-29 | 109 |
| MS.gene03936.t1 | MTR_6g033215 | 28.977 | 176 | 123 | 1 | 22 | 195 | 118 | 293 | 1.59e-26 | 103 |
| MS.gene03936.t1 | MTR_4g103530 | 46.667 | 90 | 48 | 0 | 55 | 144 | 4 | 93 | 1.76e-22 | 90.9 |
| MS.gene03936.t1 | MTR_7g011710 | 42.045 | 88 | 51 | 0 | 52 | 139 | 1 | 88 | 8.98e-20 | 82.8 |
| MS.gene03936.t1 | MTR_1g036720 | 27.103 | 107 | 76 | 1 | 5 | 109 | 4 | 110 | 7.70e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03936.t1 | AT3G31430 | 27.027 | 148 | 102 | 3 | 69 | 214 | 170 | 313 | 2.54e-14 | 71.2 |
Find 55 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGATTCTCCTTATCAAAT+AGG | 0.270270 | 3.2:+55577788 | None:intergenic |
| AAATTAAGATAAACATTCTT+AGG | 0.288740 | 3.2:+55578105 | None:intergenic |
| CAAGTGCATAACCTTCCTTT+TGG | 0.298054 | 3.2:-55578066 | MS.gene03936:CDS |
| ACCACTTAAACAAGATTTGC+AGG | 0.310094 | 3.2:-55577920 | MS.gene03936:CDS |
| GATCCAAGATGGTCGAAGTT+TGG | 0.363524 | 3.2:+55578361 | None:intergenic |
| AGGAACAGCTGGGCACTTCT+TGG | 0.363953 | 3.2:-55578284 | MS.gene03936:CDS |
| ACCTGCAAATCTTGTTTAAG+TGG | 0.371449 | 3.2:+55577919 | None:intergenic |
| ACATTCTTAGGAACCATTCC+TGG | 0.380386 | 3.2:+55578117 | None:intergenic |
| CACTTCTTGGAGGTTGGTGA+AGG | 0.396642 | 3.2:-55578271 | MS.gene03936:CDS |
| GGCTTATCTCAAGGAACAGC+TGG | 0.405289 | 3.2:-55578295 | MS.gene03936:CDS |
| AAAATATCTTTCTAAAAGAC+TGG | 0.412326 | 3.2:+55577769 | None:intergenic |
| AGGTATATGTTTCCAGTTGT+AGG | 0.418631 | 3.2:+55578234 | None:intergenic |
| TGGTAAGTACCTTGGAGAAC+TGG | 0.421913 | 3.2:-55578013 | MS.gene03936:CDS |
| TCTTGTGAGTGAATTGAAAC+AGG | 0.428370 | 3.2:+55578214 | None:intergenic |
| GTCATTGAAATTATTGCTCC+AGG | 0.433628 | 3.2:-55578135 | MS.gene03936:CDS |
| TTGTGAAATCCTATTTGATA+AGG | 0.433773 | 3.2:-55577797 | MS.gene03936:CDS |
| ATCACAGTTATACCTACAAC+TGG | 0.439649 | 3.2:-55578246 | MS.gene03936:CDS |
| TGTCCAAACTTCGACCATCT+TGG | 0.441314 | 3.2:-55578364 | MS.gene03936:CDS |
| GACAGATGCGTCAAGGTTCA+AGG | 0.450026 | 3.2:+55578383 | None:intergenic |
| TTTCACAAGTACGGTCAGTA+TGG | 0.457126 | 3.2:+55577812 | None:intergenic |
| AGCCAATCGCACACCCTTGT+CGG | 0.463403 | 3.2:+55578314 | None:intergenic |
| CGATCTTGATGGTTTGACCT+TGG | 0.465702 | 3.2:-55578421 | MS.gene03936:CDS |
| TCTTGATGGTTTGACCTTGG+AGG | 0.471139 | 3.2:-55578418 | MS.gene03936:CDS |
| ACCATGGATATCGATCTTGA+TGG | 0.471723 | 3.2:-55578432 | MS.gene03936:CDS |
| AACAGCTGGGCACTTCTTGG+AGG | 0.481954 | 3.2:-55578281 | MS.gene03936:CDS |
| TTTATTCTCGTGGTGGAGAG+TGG | 0.489142 | 3.2:-55577897 | MS.gene03936:CDS |
| ATCCGACAAGGGTGTGCGAT+TGG | 0.501301 | 3.2:-55578316 | MS.gene03936:CDS |
| CAAATAGGATTTCACAAGTA+CGG | 0.516989 | 3.2:+55577803 | None:intergenic |
| GCTGGGCACTTCTTGGAGGT+TGG | 0.520282 | 3.2:-55578277 | MS.gene03936:CDS |
| GTTGTATAAACCCAAAAGGA+AGG | 0.521586 | 3.2:+55578055 | None:intergenic |
| TGAAATTATTGCTCCAGGAA+TGG | 0.527151 | 3.2:-55578130 | MS.gene03936:CDS |
| TGGTTTGACCTTGGAGGACG+AGG | 0.528611 | 3.2:-55578412 | MS.gene03936:CDS |
| ACTTCTTGGAGGTTGGTGAA+GGG | 0.533895 | 3.2:-55578270 | MS.gene03936:CDS |
| ATCTTGGATCATTGTCTGAT+CGG | 0.544005 | 3.2:-55578348 | MS.gene03936:CDS |
| CAGTTTGTTCACGAAGCGAA+AGG | 0.544626 | 3.2:-55577962 | MS.gene03936:CDS |
| TTATTCTCGTGGTGGAGAGT+GGG | 0.553171 | 3.2:-55577896 | MS.gene03936:CDS |
| GATTTGCAGGTTTATTCTCG+TGG | 0.556064 | 3.2:-55577907 | MS.gene03936:CDS |
| ACATGATGAAGGGTGGACCT+TGG | 0.561440 | 3.2:-55578179 | MS.gene03936:CDS |
| GAAGCCATTGGTAAGTACCT+TGG | 0.564143 | 3.2:-55578021 | MS.gene03936:CDS |
| TTCTCCAAGGTACTTACCAA+TGG | 0.565889 | 3.2:+55578017 | None:intergenic |
| TGACGCTGAAAACATGATGA+AGG | 0.566730 | 3.2:-55578190 | MS.gene03936:CDS |
| CGGATGCATGCTATCCGACA+AGG | 0.576689 | 3.2:-55578328 | MS.gene03936:CDS |
| GGTCATAACCTCGTCCTCCA+AGG | 0.596650 | 3.2:+55578404 | None:intergenic |
| TGTGCGATTGGCTTATCTCA+AGG | 0.596876 | 3.2:-55578304 | MS.gene03936:CDS |
| TTGCAGGTTTATTCTCGTGG+TGG | 0.604086 | 3.2:-55577904 | MS.gene03936:CDS |
| AAGTTTGGACAGATGCGTCA+AGG | 0.608480 | 3.2:+55578376 | None:intergenic |
| CAAAAGGTAAGTGAAGCCAT+TGG | 0.619195 | 3.2:-55578033 | MS.gene03936:CDS |
| GATCAGACAATGATCCAAGA+TGG | 0.620594 | 3.2:+55578350 | None:intergenic |
| TTATAGCTGTCATACGTCCA+AGG | 0.629226 | 3.2:+55578162 | None:intergenic |
| CATACTCGACCAGTTCTCCA+AGG | 0.638239 | 3.2:+55578004 | None:intergenic |
| ACCATCAAGATCGATATCCA+TGG | 0.640079 | 3.2:+55578431 | None:intergenic |
| GCTTATCTCAAGGAACAGCT+GGG | 0.641126 | 3.2:-55578294 | MS.gene03936:CDS |
| GACGCTGAAAACATGATGAA+GGG | 0.686342 | 3.2:-55578189 | MS.gene03936:CDS |
| GGATGCATGCTATCCGACAA+GGG | 0.696542 | 3.2:-55578327 | MS.gene03936:CDS |
| GCTGAAAACATGATGAAGGG+TGG | 0.699446 | 3.2:-55578186 | MS.gene03936:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAGATAAACATTCTT+AGG | + | chr3.2:55578107-55578126 | None:intergenic | 15.0% |
| ! | TTGTGAAATCCTATTTGATA+AGG | - | chr3.2:55578412-55578431 | MS.gene03936:CDS | 25.0% |
| !! | CGATTTTGTTCAAATACAAA+AGG | - | chr3.2:55578339-55578358 | MS.gene03936:CDS | 25.0% |
| !!! | TTTTGGGTTTATACAACAAA+AGG | - | chr3.2:55578160-55578179 | MS.gene03936:CDS | 25.0% |
| !!! | TTTTGTTGTATAAACCCAAA+AGG | + | chr3.2:55578161-55578180 | None:intergenic | 25.0% |
| CAAATAGGATTTCACAAGTA+CGG | + | chr3.2:55578409-55578428 | None:intergenic | 30.0% | |
| ACCACTTAAACAAGATTTGC+AGG | - | chr3.2:55578289-55578308 | MS.gene03936:CDS | 35.0% | |
| AGGTATATGTTTCCAGTTGT+AGG | + | chr3.2:55577978-55577997 | None:intergenic | 35.0% | |
| ATCACAGTTATACCTACAAC+TGG | - | chr3.2:55577963-55577982 | MS.gene03936:CDS | 35.0% | |
| ATCTTGGATCATTGTCTGAT+CGG | - | chr3.2:55577861-55577880 | MS.gene03936:CDS | 35.0% | |
| CTGGATTCTCCTTATCAAAT+AGG | + | chr3.2:55578424-55578443 | None:intergenic | 35.0% | |
| GTCATTGAAATTATTGCTCC+AGG | - | chr3.2:55578074-55578093 | MS.gene03936:CDS | 35.0% | |
| GTTGTATAAACCCAAAAGGA+AGG | + | chr3.2:55578157-55578176 | None:intergenic | 35.0% | |
| TCTTGTGAGTGAATTGAAAC+AGG | + | chr3.2:55577998-55578017 | None:intergenic | 35.0% | |
| TGAAATTATTGCTCCAGGAA+TGG | - | chr3.2:55578079-55578098 | MS.gene03936:CDS | 35.0% | |
| ! | AAGTGCATAACCTTCCTTTT+GGG | - | chr3.2:55578144-55578163 | MS.gene03936:CDS | 35.0% |
| ! | ACCTGCAAATCTTGTTTAAG+TGG | + | chr3.2:55578293-55578312 | None:intergenic | 35.0% |
| !! | GAAACTTTTTGCTATGTGTG+TGG | - | chr3.2:55578365-55578384 | MS.gene03936:CDS | 35.0% |
| ACATTCTTAGGAACCATTCC+TGG | + | chr3.2:55578095-55578114 | None:intergenic | 40.0% | |
| CAAAAGGTAAGTGAAGCCAT+TGG | - | chr3.2:55578176-55578195 | MS.gene03936:CDS | 40.0% | |
| GACGCTGAAAACATGATGAA+GGG | - | chr3.2:55578020-55578039 | MS.gene03936:CDS | 40.0% | |
| GATCAGACAATGATCCAAGA+TGG | + | chr3.2:55577862-55577881 | None:intergenic | 40.0% | |
| GATTTGCAGGTTTATTCTCG+TGG | - | chr3.2:55578302-55578321 | MS.gene03936:CDS | 40.0% | |
| TGACGCTGAAAACATGATGA+AGG | - | chr3.2:55578019-55578038 | MS.gene03936:CDS | 40.0% | |
| TTATAGCTGTCATACGTCCA+AGG | + | chr3.2:55578050-55578069 | None:intergenic | 40.0% | |
| TTTCACAAGTACGGTCAGTA+TGG | + | chr3.2:55578400-55578419 | None:intergenic | 40.0% | |
| ! | CAAGTGCATAACCTTCCTTT+TGG | - | chr3.2:55578143-55578162 | MS.gene03936:CDS | 40.0% |
| ! | TTCTCCAAGGTACTTACCAA+TGG | + | chr3.2:55578195-55578214 | None:intergenic | 40.0% |
| AAGTTTGGACAGATGCGTCA+AGG | + | chr3.2:55577836-55577855 | None:intergenic | 45.0% | |
| GAAGCCATTGGTAAGTACCT+TGG | - | chr3.2:55578188-55578207 | MS.gene03936:CDS | 45.0% | |
| GATCCAAGATGGTCGAAGTT+TGG | + | chr3.2:55577851-55577870 | None:intergenic | 45.0% | |
| GCTGAAAACATGATGAAGGG+TGG | - | chr3.2:55578023-55578042 | MS.gene03936:CDS | 45.0% | |
| GCTTATCTCAAGGAACAGCT+GGG | - | chr3.2:55577915-55577934 | MS.gene03936:CDS | 45.0% | |
| TGGTAAGTACCTTGGAGAAC+TGG | - | chr3.2:55578196-55578215 | MS.gene03936:CDS | 45.0% | |
| TGTCCAAACTTCGACCATCT+TGG | - | chr3.2:55577845-55577864 | MS.gene03936:CDS | 45.0% | |
| TTGCAGGTTTATTCTCGTGG+TGG | - | chr3.2:55578305-55578324 | MS.gene03936:CDS | 45.0% | |
| ! | CAGTTTGTTCACGAAGCGAA+AGG | - | chr3.2:55578247-55578266 | MS.gene03936:CDS | 45.0% |
| ! | CGATCTTGATGGTTTGACCT+TGG | - | chr3.2:55577788-55577807 | MS.gene03936:CDS | 45.0% |
| ! | TCTTGATGGTTTGACCTTGG+AGG | - | chr3.2:55577791-55577810 | MS.gene03936:CDS | 45.0% |
| ! | TTATTCTCGTGGTGGAGAGT+GGG | - | chr3.2:55578313-55578332 | MS.gene03936:CDS | 45.0% |
| ! | TTTATTCTCGTGGTGGAGAG+TGG | - | chr3.2:55578312-55578331 | MS.gene03936:CDS | 45.0% |
| !! | ACTTCTTGGAGGTTGGTGAA+GGG | - | chr3.2:55577939-55577958 | MS.gene03936:CDS | 45.0% |
| !! | TGTGCGATTGGCTTATCTCA+AGG | - | chr3.2:55577905-55577924 | MS.gene03936:CDS | 45.0% |
| ACATGATGAAGGGTGGACCT+TGG | - | chr3.2:55578030-55578049 | MS.gene03936:CDS | 50.0% | |
| CATACTCGACCAGTTCTCCA+AGG | + | chr3.2:55578208-55578227 | None:intergenic | 50.0% | |
| GACAGATGCGTCAAGGTTCA+AGG | + | chr3.2:55577829-55577848 | None:intergenic | 50.0% | |
| GGATGCATGCTATCCGACAA+GGG | - | chr3.2:55577882-55577901 | MS.gene03936:CDS | 50.0% | |
| GGCTTATCTCAAGGAACAGC+TGG | - | chr3.2:55577914-55577933 | MS.gene03936:CDS | 50.0% | |
| !! | CACTTCTTGGAGGTTGGTGA+AGG | - | chr3.2:55577938-55577957 | MS.gene03936:CDS | 50.0% |
| AACAGCTGGGCACTTCTTGG+AGG | - | chr3.2:55577928-55577947 | MS.gene03936:CDS | 55.0% | |
| AGCCAATCGCACACCCTTGT+CGG | + | chr3.2:55577898-55577917 | None:intergenic | 55.0% | |
| AGGAACAGCTGGGCACTTCT+TGG | - | chr3.2:55577925-55577944 | MS.gene03936:CDS | 55.0% | |
| CGGATGCATGCTATCCGACA+AGG | - | chr3.2:55577881-55577900 | MS.gene03936:CDS | 55.0% | |
| GGTCATAACCTCGTCCTCCA+AGG | + | chr3.2:55577808-55577827 | None:intergenic | 55.0% | |
| TGGTTTGACCTTGGAGGACG+AGG | - | chr3.2:55577797-55577816 | MS.gene03936:CDS | 55.0% | |
| ! | ATCCGACAAGGGTGTGCGAT+TGG | - | chr3.2:55577893-55577912 | MS.gene03936:CDS | 55.0% |
| GCTGGGCACTTCTTGGAGGT+TGG | - | chr3.2:55577932-55577951 | MS.gene03936:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 55577780 | 55578451 | 55577780 | ID=MS.gene03936 |
| chr3.2 | mRNA | 55577780 | 55578451 | 55577780 | ID=MS.gene03936.t1;Parent=MS.gene03936 |
| chr3.2 | exon | 55577780 | 55578451 | 55577780 | ID=MS.gene03936.t1.exon1;Parent=MS.gene03936.t1 |
| chr3.2 | CDS | 55577780 | 55578451 | 55577780 | ID=cds.MS.gene03936.t1;Parent=MS.gene03936.t1 |
| Gene Sequence |
| Protein sequence |