AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03936


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03936.t1 MTR_3g053920 37.423 163 87 1 2 164 5 152 9.04e-38 133
MS.gene03936.t1 MTR_2g061500 41.129 124 73 0 3 126 6 129 3.42e-33 117
MS.gene03936.t1 MTR_0010s0370 32.000 175 115 2 4 174 3 177 1.41e-29 109
MS.gene03936.t1 MTR_0010s0350 32.000 175 115 2 4 174 3 177 1.41e-29 109
MS.gene03936.t1 MTR_6g033215 28.977 176 123 1 22 195 118 293 1.59e-26 103
MS.gene03936.t1 MTR_4g103530 46.667 90 48 0 55 144 4 93 1.76e-22 90.9
MS.gene03936.t1 MTR_7g011710 42.045 88 51 0 52 139 1 88 8.98e-20 82.8
MS.gene03936.t1 MTR_1g036720 27.103 107 76 1 5 109 4 110 7.70e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03936.t1 AT3G31430 27.027 148 102 3 69 214 170 313 2.54e-14 71.2

Find 55 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGATTCTCCTTATCAAAT+AGG 0.270270 3.2:+55577788 None:intergenic
AAATTAAGATAAACATTCTT+AGG 0.288740 3.2:+55578105 None:intergenic
CAAGTGCATAACCTTCCTTT+TGG 0.298054 3.2:-55578066 MS.gene03936:CDS
ACCACTTAAACAAGATTTGC+AGG 0.310094 3.2:-55577920 MS.gene03936:CDS
GATCCAAGATGGTCGAAGTT+TGG 0.363524 3.2:+55578361 None:intergenic
AGGAACAGCTGGGCACTTCT+TGG 0.363953 3.2:-55578284 MS.gene03936:CDS
ACCTGCAAATCTTGTTTAAG+TGG 0.371449 3.2:+55577919 None:intergenic
ACATTCTTAGGAACCATTCC+TGG 0.380386 3.2:+55578117 None:intergenic
CACTTCTTGGAGGTTGGTGA+AGG 0.396642 3.2:-55578271 MS.gene03936:CDS
GGCTTATCTCAAGGAACAGC+TGG 0.405289 3.2:-55578295 MS.gene03936:CDS
AAAATATCTTTCTAAAAGAC+TGG 0.412326 3.2:+55577769 None:intergenic
AGGTATATGTTTCCAGTTGT+AGG 0.418631 3.2:+55578234 None:intergenic
TGGTAAGTACCTTGGAGAAC+TGG 0.421913 3.2:-55578013 MS.gene03936:CDS
TCTTGTGAGTGAATTGAAAC+AGG 0.428370 3.2:+55578214 None:intergenic
GTCATTGAAATTATTGCTCC+AGG 0.433628 3.2:-55578135 MS.gene03936:CDS
TTGTGAAATCCTATTTGATA+AGG 0.433773 3.2:-55577797 MS.gene03936:CDS
ATCACAGTTATACCTACAAC+TGG 0.439649 3.2:-55578246 MS.gene03936:CDS
TGTCCAAACTTCGACCATCT+TGG 0.441314 3.2:-55578364 MS.gene03936:CDS
GACAGATGCGTCAAGGTTCA+AGG 0.450026 3.2:+55578383 None:intergenic
TTTCACAAGTACGGTCAGTA+TGG 0.457126 3.2:+55577812 None:intergenic
AGCCAATCGCACACCCTTGT+CGG 0.463403 3.2:+55578314 None:intergenic
CGATCTTGATGGTTTGACCT+TGG 0.465702 3.2:-55578421 MS.gene03936:CDS
TCTTGATGGTTTGACCTTGG+AGG 0.471139 3.2:-55578418 MS.gene03936:CDS
ACCATGGATATCGATCTTGA+TGG 0.471723 3.2:-55578432 MS.gene03936:CDS
AACAGCTGGGCACTTCTTGG+AGG 0.481954 3.2:-55578281 MS.gene03936:CDS
TTTATTCTCGTGGTGGAGAG+TGG 0.489142 3.2:-55577897 MS.gene03936:CDS
ATCCGACAAGGGTGTGCGAT+TGG 0.501301 3.2:-55578316 MS.gene03936:CDS
CAAATAGGATTTCACAAGTA+CGG 0.516989 3.2:+55577803 None:intergenic
GCTGGGCACTTCTTGGAGGT+TGG 0.520282 3.2:-55578277 MS.gene03936:CDS
GTTGTATAAACCCAAAAGGA+AGG 0.521586 3.2:+55578055 None:intergenic
TGAAATTATTGCTCCAGGAA+TGG 0.527151 3.2:-55578130 MS.gene03936:CDS
TGGTTTGACCTTGGAGGACG+AGG 0.528611 3.2:-55578412 MS.gene03936:CDS
ACTTCTTGGAGGTTGGTGAA+GGG 0.533895 3.2:-55578270 MS.gene03936:CDS
ATCTTGGATCATTGTCTGAT+CGG 0.544005 3.2:-55578348 MS.gene03936:CDS
CAGTTTGTTCACGAAGCGAA+AGG 0.544626 3.2:-55577962 MS.gene03936:CDS
TTATTCTCGTGGTGGAGAGT+GGG 0.553171 3.2:-55577896 MS.gene03936:CDS
GATTTGCAGGTTTATTCTCG+TGG 0.556064 3.2:-55577907 MS.gene03936:CDS
ACATGATGAAGGGTGGACCT+TGG 0.561440 3.2:-55578179 MS.gene03936:CDS
GAAGCCATTGGTAAGTACCT+TGG 0.564143 3.2:-55578021 MS.gene03936:CDS
TTCTCCAAGGTACTTACCAA+TGG 0.565889 3.2:+55578017 None:intergenic
TGACGCTGAAAACATGATGA+AGG 0.566730 3.2:-55578190 MS.gene03936:CDS
CGGATGCATGCTATCCGACA+AGG 0.576689 3.2:-55578328 MS.gene03936:CDS
GGTCATAACCTCGTCCTCCA+AGG 0.596650 3.2:+55578404 None:intergenic
TGTGCGATTGGCTTATCTCA+AGG 0.596876 3.2:-55578304 MS.gene03936:CDS
TTGCAGGTTTATTCTCGTGG+TGG 0.604086 3.2:-55577904 MS.gene03936:CDS
AAGTTTGGACAGATGCGTCA+AGG 0.608480 3.2:+55578376 None:intergenic
CAAAAGGTAAGTGAAGCCAT+TGG 0.619195 3.2:-55578033 MS.gene03936:CDS
GATCAGACAATGATCCAAGA+TGG 0.620594 3.2:+55578350 None:intergenic
TTATAGCTGTCATACGTCCA+AGG 0.629226 3.2:+55578162 None:intergenic
CATACTCGACCAGTTCTCCA+AGG 0.638239 3.2:+55578004 None:intergenic
ACCATCAAGATCGATATCCA+TGG 0.640079 3.2:+55578431 None:intergenic
GCTTATCTCAAGGAACAGCT+GGG 0.641126 3.2:-55578294 MS.gene03936:CDS
GACGCTGAAAACATGATGAA+GGG 0.686342 3.2:-55578189 MS.gene03936:CDS
GGATGCATGCTATCCGACAA+GGG 0.696542 3.2:-55578327 MS.gene03936:CDS
GCTGAAAACATGATGAAGGG+TGG 0.699446 3.2:-55578186 MS.gene03936:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAGATAAACATTCTT+AGG + chr3.2:55578107-55578126 None:intergenic 15.0%
! TTGTGAAATCCTATTTGATA+AGG - chr3.2:55578412-55578431 MS.gene03936:CDS 25.0%
!! CGATTTTGTTCAAATACAAA+AGG - chr3.2:55578339-55578358 MS.gene03936:CDS 25.0%
!!! TTTTGGGTTTATACAACAAA+AGG - chr3.2:55578160-55578179 MS.gene03936:CDS 25.0%
!!! TTTTGTTGTATAAACCCAAA+AGG + chr3.2:55578161-55578180 None:intergenic 25.0%
CAAATAGGATTTCACAAGTA+CGG + chr3.2:55578409-55578428 None:intergenic 30.0%
ACCACTTAAACAAGATTTGC+AGG - chr3.2:55578289-55578308 MS.gene03936:CDS 35.0%
AGGTATATGTTTCCAGTTGT+AGG + chr3.2:55577978-55577997 None:intergenic 35.0%
ATCACAGTTATACCTACAAC+TGG - chr3.2:55577963-55577982 MS.gene03936:CDS 35.0%
ATCTTGGATCATTGTCTGAT+CGG - chr3.2:55577861-55577880 MS.gene03936:CDS 35.0%
CTGGATTCTCCTTATCAAAT+AGG + chr3.2:55578424-55578443 None:intergenic 35.0%
GTCATTGAAATTATTGCTCC+AGG - chr3.2:55578074-55578093 MS.gene03936:CDS 35.0%
GTTGTATAAACCCAAAAGGA+AGG + chr3.2:55578157-55578176 None:intergenic 35.0%
TCTTGTGAGTGAATTGAAAC+AGG + chr3.2:55577998-55578017 None:intergenic 35.0%
TGAAATTATTGCTCCAGGAA+TGG - chr3.2:55578079-55578098 MS.gene03936:CDS 35.0%
! AAGTGCATAACCTTCCTTTT+GGG - chr3.2:55578144-55578163 MS.gene03936:CDS 35.0%
! ACCTGCAAATCTTGTTTAAG+TGG + chr3.2:55578293-55578312 None:intergenic 35.0%
!! GAAACTTTTTGCTATGTGTG+TGG - chr3.2:55578365-55578384 MS.gene03936:CDS 35.0%
ACATTCTTAGGAACCATTCC+TGG + chr3.2:55578095-55578114 None:intergenic 40.0%
CAAAAGGTAAGTGAAGCCAT+TGG - chr3.2:55578176-55578195 MS.gene03936:CDS 40.0%
GACGCTGAAAACATGATGAA+GGG - chr3.2:55578020-55578039 MS.gene03936:CDS 40.0%
GATCAGACAATGATCCAAGA+TGG + chr3.2:55577862-55577881 None:intergenic 40.0%
GATTTGCAGGTTTATTCTCG+TGG - chr3.2:55578302-55578321 MS.gene03936:CDS 40.0%
TGACGCTGAAAACATGATGA+AGG - chr3.2:55578019-55578038 MS.gene03936:CDS 40.0%
TTATAGCTGTCATACGTCCA+AGG + chr3.2:55578050-55578069 None:intergenic 40.0%
TTTCACAAGTACGGTCAGTA+TGG + chr3.2:55578400-55578419 None:intergenic 40.0%
! CAAGTGCATAACCTTCCTTT+TGG - chr3.2:55578143-55578162 MS.gene03936:CDS 40.0%
! TTCTCCAAGGTACTTACCAA+TGG + chr3.2:55578195-55578214 None:intergenic 40.0%
AAGTTTGGACAGATGCGTCA+AGG + chr3.2:55577836-55577855 None:intergenic 45.0%
GAAGCCATTGGTAAGTACCT+TGG - chr3.2:55578188-55578207 MS.gene03936:CDS 45.0%
GATCCAAGATGGTCGAAGTT+TGG + chr3.2:55577851-55577870 None:intergenic 45.0%
GCTGAAAACATGATGAAGGG+TGG - chr3.2:55578023-55578042 MS.gene03936:CDS 45.0%
GCTTATCTCAAGGAACAGCT+GGG - chr3.2:55577915-55577934 MS.gene03936:CDS 45.0%
TGGTAAGTACCTTGGAGAAC+TGG - chr3.2:55578196-55578215 MS.gene03936:CDS 45.0%
TGTCCAAACTTCGACCATCT+TGG - chr3.2:55577845-55577864 MS.gene03936:CDS 45.0%
TTGCAGGTTTATTCTCGTGG+TGG - chr3.2:55578305-55578324 MS.gene03936:CDS 45.0%
! CAGTTTGTTCACGAAGCGAA+AGG - chr3.2:55578247-55578266 MS.gene03936:CDS 45.0%
! CGATCTTGATGGTTTGACCT+TGG - chr3.2:55577788-55577807 MS.gene03936:CDS 45.0%
! TCTTGATGGTTTGACCTTGG+AGG - chr3.2:55577791-55577810 MS.gene03936:CDS 45.0%
! TTATTCTCGTGGTGGAGAGT+GGG - chr3.2:55578313-55578332 MS.gene03936:CDS 45.0%
! TTTATTCTCGTGGTGGAGAG+TGG - chr3.2:55578312-55578331 MS.gene03936:CDS 45.0%
!! ACTTCTTGGAGGTTGGTGAA+GGG - chr3.2:55577939-55577958 MS.gene03936:CDS 45.0%
!! TGTGCGATTGGCTTATCTCA+AGG - chr3.2:55577905-55577924 MS.gene03936:CDS 45.0%
ACATGATGAAGGGTGGACCT+TGG - chr3.2:55578030-55578049 MS.gene03936:CDS 50.0%
CATACTCGACCAGTTCTCCA+AGG + chr3.2:55578208-55578227 None:intergenic 50.0%
GACAGATGCGTCAAGGTTCA+AGG + chr3.2:55577829-55577848 None:intergenic 50.0%
GGATGCATGCTATCCGACAA+GGG - chr3.2:55577882-55577901 MS.gene03936:CDS 50.0%
GGCTTATCTCAAGGAACAGC+TGG - chr3.2:55577914-55577933 MS.gene03936:CDS 50.0%
!! CACTTCTTGGAGGTTGGTGA+AGG - chr3.2:55577938-55577957 MS.gene03936:CDS 50.0%
AACAGCTGGGCACTTCTTGG+AGG - chr3.2:55577928-55577947 MS.gene03936:CDS 55.0%
AGCCAATCGCACACCCTTGT+CGG + chr3.2:55577898-55577917 None:intergenic 55.0%
AGGAACAGCTGGGCACTTCT+TGG - chr3.2:55577925-55577944 MS.gene03936:CDS 55.0%
CGGATGCATGCTATCCGACA+AGG - chr3.2:55577881-55577900 MS.gene03936:CDS 55.0%
GGTCATAACCTCGTCCTCCA+AGG + chr3.2:55577808-55577827 None:intergenic 55.0%
TGGTTTGACCTTGGAGGACG+AGG - chr3.2:55577797-55577816 MS.gene03936:CDS 55.0%
! ATCCGACAAGGGTGTGCGAT+TGG - chr3.2:55577893-55577912 MS.gene03936:CDS 55.0%
GCTGGGCACTTCTTGGAGGT+TGG - chr3.2:55577932-55577951 MS.gene03936:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 55577780 55578451 55577780 ID=MS.gene03936
chr3.2 mRNA 55577780 55578451 55577780 ID=MS.gene03936.t1;Parent=MS.gene03936
chr3.2 exon 55577780 55578451 55577780 ID=MS.gene03936.t1.exon1;Parent=MS.gene03936.t1
chr3.2 CDS 55577780 55578451 55577780 ID=cds.MS.gene03936.t1;Parent=MS.gene03936.t1
Gene Sequence

>MS.gene03936

ATGGATATCGATCTTGATGGTTTGACCTTGGAGGACGAGGTTATGACCTTGAACCTTGACGCATCTGTCCAAACTTCGACCATCTTGGATCATTGTCTGATCGGATGCATGCTATCCGACAAGGGTGTGCGATTGGCTTATCTCAAGGAACAGCTGGGCACTTCTTGGAGGTTGGTGAAGGGTATCACAGTTATACCTACAACTGGAAACATATACCTGTTTCAATTCACTCACAAGAATGACGCTGAAAACATGATGAAGGGTGGACCTTGGACGTATGACAGCTATAATCTAGTCATTGAAATTATTGCTCCAGGAATGGTTCCTAAGAATGTTTATCTTAATTTTCTGAACATTTGTGTGCAAGTGCATAACCTTCCTTTTGGGTTTATACAACAAAAGGTAAGTGAAGCCATTGGTAAGTACCTTGGAGAACTGGTCGAGTATGACAGTAAGAACACTATTCACAGTTTGTTCACGAAGCGAAAGGTTCGAATCAATGTAATACAACCACTTAAACAAGATTTGCAGGTTTATTCTCGTGGTGGAGAGTGGGTTTCGATTTTGTTCAAATACAAAAGGCTTGAAACTTTTTGCTATGTGTGTGGAGTTCTTGACCATACTGACCGTACTTGTGAAATCCTATTTGATAAGGAGAATCCAGTCTTTTAG

Protein sequence

>MS.gene03936.t1

MDIDLDGLTLEDEVMTLNLDASVQTSTILDHCLIGCMLSDKGVRLAYLKEQLGTSWRLVKGITVIPTTGNIYLFQFTHKNDAENMMKGGPWTYDSYNLVIEIIAPGMVPKNVYLNFLNICVQVHNLPFGFIQQKVSEAIGKYLGELVEYDSKNTIHSLFTKRKVRINVIQPLKQDLQVYSRGGEWVSILFKYKRLETFCYVCGVLDHTDRTCEILFDKENPVF