AlfalfaGEDB | MS.gene012889

M. sativa cultivar XinJiangDaYe / MS.gene012889

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012889.t1	XP_024630779.1	79.2	265	48	2	1	259	1	264	1.00E-122	449.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012889.t1	A0A396JIW9	76.1	243	58	0	1	243	1	243	1.2e-109	405.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene021965	MS.gene012889	PPI
MS.gene055683	MS.gene012889	PPI
MS.gene042614	MS.gene012889	PPI
MS.gene41643	MS.gene012889	PPI
MS.gene013890	MS.gene012889	PPI
MS.gene26844	MS.gene012889	PPI
MS.gene021555	MS.gene012889	PPI
MS.gene012889	MS.gene65474	PPI
MS.gene060773	MS.gene012889	PPI
MS.gene021647	MS.gene012889	PPI
MS.gene012889	MS.gene025401	PPI
MS.gene96109	MS.gene012889	PPI
MS.gene69584	MS.gene012889	PPI
MS.gene012889	MS.gene015093	PPI
MS.gene019165	MS.gene012889	PPI
MS.gene048706	MS.gene012889	PPI
MS.gene012889	MS.gene000379	PPI
MS.gene012889	MS.gene032299	PPI
MS.gene009121	MS.gene012889	PPI
MS.gene012889	MS.gene08606	PPI
MS.gene94654	MS.gene012889	PPI
MS.gene024841	MS.gene012889	PPI
MS.gene51804	MS.gene012889	PPI
MS.gene87845	MS.gene012889	PPI
MS.gene36069	MS.gene012889	PPI
MS.gene012889	MS.gene09517	PPI
MS.gene012889	MS.gene98756	PPI
MS.gene95960	MS.gene012889	PPI
MS.gene61452	MS.gene012889	PPI
MS.gene83772	MS.gene012889	PPI
MS.gene009213	MS.gene012889	PPI
MS.gene008927	MS.gene012889	PPI
MS.gene016102	MS.gene012889	PPI
MS.gene045614	MS.gene012889	PPI
MS.gene96541	MS.gene012889	PPI
MS.gene012889	MS.gene62249	PPI
MS.gene049561	MS.gene012889	PPI
MS.gene000634	MS.gene012889	PPI
MS.gene009781	MS.gene012889	PPI
MS.gene63762	MS.gene012889	PPI
MS.gene30208	MS.gene012889	PPI
MS.gene028716	MS.gene012889	PPI
MS.gene025873	MS.gene012889	PPI
MS.gene06164	MS.gene012889	PPI
MS.gene013676	MS.gene012889	PPI
MS.gene012889	MS.gene044729	PPI
MS.gene05358	MS.gene012889	PPI
MS.gene012889	MS.gene60390	PPI
MS.gene95561	MS.gene012889	PPI
MS.gene012889	MS.gene039692	PPI
MS.gene074257	MS.gene012889	PPI
MS.gene018196	MS.gene012889	PPI
MS.gene065627	MS.gene012889	PPI
MS.gene57019	MS.gene012889	PPI
MS.gene010890	MS.gene012889	PPI
MS.gene018745	MS.gene012889	PPI
MS.gene65546	MS.gene012889	PPI
MS.gene09023	MS.gene012889	PPI
MS.gene000506	MS.gene012889	PPI
MS.gene032299	MS.gene012889	PPI
MS.gene03936	MS.gene012889	PPI
MS.gene038098	MS.gene012889	PPI
MS.gene60059	MS.gene012889	PPI
MS.gene012889	MS.gene057934	PPI
MS.gene062511	MS.gene012889	PPI
MS.gene012889	MS.gene37537	PPI
MS.gene22111	MS.gene012889	PPI
MS.gene012889	MS.gene024841	PPI
MS.gene012889	MS.gene069786	PPI
MS.gene28545	MS.gene012889	PPI
MS.gene28718	MS.gene012889	PPI
MS.gene012889	MS.gene025158	PPI
MS.gene055560	MS.gene012889	PPI
MS.gene008275	MS.gene012889	PPI
MS.gene29321	MS.gene012889	PPI
MS.gene34618	MS.gene012889	PPI
MS.gene038757	MS.gene012889	PPI
MS.gene064966	MS.gene012889	PPI
MS.gene016632	MS.gene012889	PPI
MS.gene002590	MS.gene012889	PPI
MS.gene40828	MS.gene012889	PPI
MS.gene43924	MS.gene012889	PPI
MS.gene046089	MS.gene012889	PPI
MS.gene012889	MS.gene043140	PPI
MS.gene047954	MS.gene012889	PPI
MS.gene77990	MS.gene012889	PPI
MS.gene036423	MS.gene012889	PPI
MS.gene012889	MS.gene03149	PPI
MS.gene054652	MS.gene012889	PPI
MS.gene013644	MS.gene012889	PPI
MS.gene012889	MS.gene29321	PPI
MS.gene72520	MS.gene012889	PPI
MS.gene66651	MS.gene012889	PPI
MS.gene35202	MS.gene012889	PPI
MS.gene051449	MS.gene012889	PPI
MS.gene012889	MS.gene24278	PPI
MS.gene012889	MS.gene34618	PPI
MS.gene019001	MS.gene012889	PPI
MS.gene63743	MS.gene012889	PPI
MS.gene012889	MS.gene59460	PPI
MS.gene012889	MS.gene060773	PPI
MS.gene63150	MS.gene012889	PPI
MS.gene48477	MS.gene012889	PPI
MS.gene045875	MS.gene012889	PPI
MS.gene012889	MS.gene08713	PPI
MS.gene012889	MS.gene038394	PPI
MS.gene038394	MS.gene012889	PPI
MS.gene30136	MS.gene012889	PPI
MS.gene008861	MS.gene012889	PPI
MS.gene012889	MS.gene24070	PPI
MS.gene73261	MS.gene012889	PPI
MS.gene024297	MS.gene012889	PPI
MS.gene83979	MS.gene012889	PPI
MS.gene46941	MS.gene012889	PPI
MS.gene45083	MS.gene012889	PPI
MS.gene92517	MS.gene012889	PPI
MS.gene012889	MS.gene055546	PPI
MS.gene85295	MS.gene012889	PPI
MS.gene057934	MS.gene012889	PPI
MS.gene47792	MS.gene012889	PPI
MS.gene46948	MS.gene012889	PPI
MS.gene98471	MS.gene012889	PPI
MS.gene049610	MS.gene012889	PPI
MS.gene012019	MS.gene012889	PPI
MS.gene012889	MS.gene44144	PPI
MS.gene060718	MS.gene012889	PPI
MS.gene025401	MS.gene012889	PPI
MS.gene93396	MS.gene012889	PPI
MS.gene012889	MS.gene068629	PPI
MS.gene52349	MS.gene012889	PPI
MS.gene05231	MS.gene012889	PPI
MS.gene012889	MS.gene009781	PPI
MS.gene46353	MS.gene012889	PPI
MS.gene010656	MS.gene012889	PPI
MS.gene000629	MS.gene012889	PPI
MS.gene42010	MS.gene012889	PPI
MS.gene059269	MS.gene012889	PPI
MS.gene40469	MS.gene012889	PPI
MS.gene041264	MS.gene012889	PPI
MS.gene41979	MS.gene012889	PPI
MS.gene024522	MS.gene012889	PPI
MS.gene012889	MS.gene21066	PPI
MS.gene64130	MS.gene012889	PPI
MS.gene05319	MS.gene012889	PPI
MS.gene012889	MS.gene98127	PPI
MS.gene56991	MS.gene012889	PPI
MS.gene050679	MS.gene012889	PPI
MS.gene012889	MS.gene003264	PPI
MS.gene85538	MS.gene012889	PPI
MS.gene20246	MS.gene012889	PPI
MS.gene055223	MS.gene012889	PPI
MS.gene016719	MS.gene012889	PPI
MS.gene010290	MS.gene012889	PPI
MS.gene053747	MS.gene012889	PPI
MS.gene012889	MS.gene27329	PPI
MS.gene012889	MS.gene41393	PPI
MS.gene65471	MS.gene012889	PPI
MS.gene061850	MS.gene012889	PPI
MS.gene26433	MS.gene012889	PPI
MS.gene012889	MS.gene016632	PPI
MS.gene34463	MS.gene012889	PPI
MS.gene012889	MS.gene018474	PPI
MS.gene012889	MS.gene048088	PPI
MS.gene048088	MS.gene012889	PPI
MS.gene012889	MS.gene86868	PPI
MS.gene22730	MS.gene012889	PPI
MS.gene003264	MS.gene012889	PPI
MS.gene073582	MS.gene012889	PPI
MS.gene012889	MS.gene96163	PPI
MS.gene058889	MS.gene012889	PPI
MS.gene98616	MS.gene012889	PPI
MS.gene53904	MS.gene012889	PPI
MS.gene08708	MS.gene012889	PPI
MS.gene69586	MS.gene012889	PPI
MS.gene042027	MS.gene012889	PPI
MS.gene85308	MS.gene012889	PPI
MS.gene69490	MS.gene012889	PPI
MS.gene015248	MS.gene012889	PPI
MS.gene80362	MS.gene012889	PPI
MS.gene44083	MS.gene012889	PPI
MS.gene013193	MS.gene012889	PPI
MS.gene04970	MS.gene012889	PPI
MS.gene08106	MS.gene012889	PPI
MS.gene012889	MS.gene61499	PPI
MS.gene51799	MS.gene012889	PPI
MS.gene58419	MS.gene012889	PPI
MS.gene96163	MS.gene012889	PPI
MS.gene012889	MS.gene81194	PPI
MS.gene08713	MS.gene012889	PPI
MS.gene23521	MS.gene012889	PPI
MS.gene29406	MS.gene012889	PPI
MS.gene54051	MS.gene012889	PPI
MS.gene86275	MS.gene012889	PPI
MS.gene98127	MS.gene012889	PPI
MS.gene87529	MS.gene012889	PPI
MS.gene024405	MS.gene012889	PPI
MS.gene012748	MS.gene012889	PPI
MS.gene04134	MS.gene012889	PPI
MS.gene062067	MS.gene012889	PPI
MS.gene57044	MS.gene012889	PPI
MS.gene054508	MS.gene012889	PPI
MS.gene89815	MS.gene012889	PPI
MS.gene028714	MS.gene012889	PPI
MS.gene017586	MS.gene012889	PPI
MS.gene038676	MS.gene012889	PPI
MS.gene98756	MS.gene012889	PPI
MS.gene71637	MS.gene012889	PPI
MS.gene012889	MS.gene022929	PPI
MS.gene042651	MS.gene012889	PPI
MS.gene012889	MS.gene007022	PPI
MS.gene05576	MS.gene012889	PPI
MS.gene98467	MS.gene012889	PPI
MS.gene037049	MS.gene012889	PPI
MS.gene012889	MS.gene048342	PPI
MS.gene034643	MS.gene012889	PPI
MS.gene044729	MS.gene012889	PPI
MS.gene012889	MS.gene069624	PPI
MS.gene037634	MS.gene012889	PPI
MS.gene24070	MS.gene012889	PPI
MS.gene012889	MS.gene88393	PPI
MS.gene071784	MS.gene012889	PPI
MS.gene012889	MS.gene008658	PPI
MS.gene027169	MS.gene012889	PPI
MS.gene061579	MS.gene012889	PPI
MS.gene039692	MS.gene012889	PPI
MS.gene76420	MS.gene012889	PPI
MS.gene071320	MS.gene012889	PPI
MS.gene012889	MS.gene49057	PPI
MS.gene42903	MS.gene012889	PPI
MS.gene81712	MS.gene012889	PPI
MS.gene026680	MS.gene012889	PPI
MS.gene048747	MS.gene012889	PPI
MS.gene042559	MS.gene012889	PPI
MS.gene22200	MS.gene012889	PPI
MS.gene055546	MS.gene012889	PPI
MS.gene26038	MS.gene012889	PPI
MS.gene49057	MS.gene012889	PPI
MS.gene073573	MS.gene012889	PPI
MS.gene012889	MS.gene000640	PPI
MS.gene031967	MS.gene012889	PPI
MS.gene053373	MS.gene012889	PPI
MS.gene34812	MS.gene012889	PPI
MS.gene02896	MS.gene012889	PPI
MS.gene063214	MS.gene012889	PPI
MS.gene55682	MS.gene012889	PPI
MS.gene97410	MS.gene012889	PPI
MS.gene32415	MS.gene012889	PPI
MS.gene012889	MS.gene74639	PPI
MS.gene012889	MS.gene071133	PPI
MS.gene012889	MS.gene60266	PPI
MS.gene060788	MS.gene012889	PPI
MS.gene035626	MS.gene012889	PPI
MS.gene067340	MS.gene012889	PPI
MS.gene012889	MS.gene54051	PPI
MS.gene65623	MS.gene012889	PPI
MS.gene27329	MS.gene012889	PPI
MS.gene71675	MS.gene012889	PPI
MS.gene012889	MS.gene37955	PPI
MS.gene019177	MS.gene012889	PPI
MS.gene69495	MS.gene012889	PPI
MS.gene049512	MS.gene012889	PPI
MS.gene012889	MS.gene92517	PPI
MS.gene038623	MS.gene012889	PPI
MS.gene012889	MS.gene010890	PPI
MS.gene012889	MS.gene84790	PPI
MS.gene55209	MS.gene012889	PPI
MS.gene05357	MS.gene012889	PPI
MS.gene032311	MS.gene012889	PPI
MS.gene64178	MS.gene012889	PPI
MS.gene004417	MS.gene012889	PPI
MS.gene030090	MS.gene012889	PPI
MS.gene96174	MS.gene012889	PPI
MS.gene86849	MS.gene012889	PPI
MS.gene50392	MS.gene012889	PPI
MS.gene072852	MS.gene012889	PPI
MS.gene012889	MS.gene060788	PPI
MS.gene41393	MS.gene012889	PPI
MS.gene39956	MS.gene012889	PPI
MS.gene012889	MS.gene77990	PPI
MS.gene012889	MS.gene062511	PPI
MS.gene021968	MS.gene012889	PPI
MS.gene012889	MS.gene027994	PPI
MS.gene09589	MS.gene012889	PPI
MS.gene012889	MS.gene69584	PPI
MS.gene43393	MS.gene012889	PPI
MS.gene057828	MS.gene012889	PPI
MS.gene34197	MS.gene012889	PPI
MS.gene012889	MS.gene46353	PPI
MS.gene66650	MS.gene012889	PPI
MS.gene005120	MS.gene012889	PPI
MS.gene012889	MS.gene61651	PPI
MS.gene048072	MS.gene012889	PPI
MS.gene060386	MS.gene012889	PPI
MS.gene71588	MS.gene012889	PPI
MS.gene04749	MS.gene012889	PPI
MS.gene012889	MS.gene09023	PPI
MS.gene05436	MS.gene012889	PPI
MS.gene012889	MS.gene054508	PPI
MS.gene012889	MS.gene025987	PPI
MS.gene059736	MS.gene012889	PPI
MS.gene70932	MS.gene012889	PPI
MS.gene012889	MS.gene95960	PPI
MS.gene35175	MS.gene012889	PPI
MS.gene81194	MS.gene012889	PPI
MS.gene025675	MS.gene012889	PPI
MS.gene069786	MS.gene012889	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012889.t1	MTR_0010s0370	74.863	183	46	0	1	183	1	183	6.13e-100	290
MS.gene012889.t1	MTR_0010s0350	74.863	183	46	0	1	183	1	183	6.13e-100	290
MS.gene012889.t1	MTR_1g036720	74.775	111	28	0	1	111	1	111	1.60e-60	187
MS.gene012889.t1	MTR_1g036710	48.092	131	30	2	132	258	1	97	2.65e-31	117
MS.gene012889.t1	MTR_6g033215	31.319	182	119	3	20	199	117	294	1.40e-27	107
MS.gene012889.t1	MTR_7g089990	47.222	108	55	1	104	209	86	193	2.30e-22	92.0
MS.gene012889.t1	MTR_3g053920	34.058	138	74	2	34	171	36	156	3.37e-20	87.8
MS.gene012889.t1	MTR_2g061500	37.190	121	74	1	7	127	11	129	5.59e-18	78.6
MS.gene012889.t1	MTR_4g103530	41.573	89	52	0	57	145	5	93	3.22e-17	77.8
MS.gene012889.t1	MTR_7g106180	26.897	145	99	2	34	175	26	166	2.86e-13	67.8
MS.gene012889.t1	MTR_3g024240	33.884	121	78	1	82	200	55	175	7.81e-13	65.9
MS.gene012889.t1	MTR_7g011710	34.524	84	55	0	57	140	5	88	4.52e-12	62.8
MS.gene012889.t1	MTR_0488s0020	22.707	229	143	5	43	259	133	339	9.52e-12	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012889.t1	AT3G31430	26.961	204	143	3	14	215	112	311	1.21e-19	87.0
MS.gene012889.t1	AT2G41590	27.189	217	146	5	4	215	9	218	6.80e-19	85.1
MS.gene012889.t1	AT5G36228	23.661	224	169	1	3	226	8	229	4.62e-18	82.8
MS.gene012889.t1	AT5G18636	25.926	216	150	4	4	215	9	218	5.70e-18	82.8
MS.gene012889.t1	AT2G17920	26.852	216	148	5	4	215	9	218	5.89e-18	82.0
MS.gene012889.t1	AT5G25200	25.926	216	150	4	4	215	9	218	6.54e-18	82.4
MS.gene012889.t1	AT2G13450	26.147	218	147	4	4	215	9	218	1.14e-17	82.0
MS.gene012889.t1	AT3G57587	26.087	184	134	1	32	215	37	218	2.06e-17	80.9
MS.gene012889.t1	AT2G02103	25.463	216	151	4	4	215	9	218	2.44e-17	81.3
MS.gene012889.t1	AT3G47920	31.944	144	90	3	75	215	9	147	2.09e-14	72.0
MS.gene012889.t1	AT5G36225	33.333	90	60	0	121	210	17	106	1.12e-13	68.9
MS.gene012889.t1	AT4G02000	27.857	140	89	2	81	215	6	138	2.19e-13	68.9
MS.gene012889.t1	AT2G13500	24.336	226	166	4	4	228	9	230	3.89e-11	62.8

Find 65 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTCCAATTTGTGCCGTTT+TGG	0.219874	3.3:-85000247	MS.gene012889:CDS
TGGAAAAGATTGAAACCTTT+TGG	0.248061	3.3:-85000439	MS.gene012889:CDS
CTGGTTCTACCTCTTGAATT+TGG	0.260958	3.3:+85000399	None:intergenic
CTTGATGTCCTTCCAGAAAA+TGG	0.282882	3.3:-85000287	MS.gene012889:CDS
GAGATAATTCGAGTTGTTCT+TGG	0.302396	3.3:+85000133	None:intergenic
GAACCCAAAACGGCACAAAT+TGG	0.344104	3.3:+85000243	None:intergenic
GACCCTCACCTCCGTCTTGT+TGG	0.361586	3.3:-85000503	MS.gene012889:CDS
AAGGAAATTGCAAACTTCAT+TGG	0.378123	3.3:-85000176	MS.gene012889:CDS
TGAGGATTCTCTGTAGGTCT+AGG	0.397183	3.3:+85000348	None:intergenic
AACTGTTAAACCTTTGCTGC+TGG	0.402633	3.3:+84999821	None:intergenic
TGAATTTGGACACCTTTGAC+AGG	0.420319	3.3:+85000413	None:intergenic
AATTGGAGGTTTACCTGGCT+TGG	0.438429	3.3:+85000260	None:intergenic
CTGATGATGGAACAAGACTA+TGG	0.442111	3.3:-84999920	MS.gene012889:CDS
TTGTGAAAAGCTTTATTCCA+TGG	0.453343	3.3:-84999946	MS.gene012889:CDS
TTGGCCGCTTCCTCACTAAC+CGG	0.476388	3.3:-85000484	MS.gene012889:CDS
CTTTCTTGAGGATTCTCTGT+AGG	0.482296	3.3:+85000342	None:intergenic
TATTCCATGGAATCTGATGA+TGG	0.485852	3.3:-84999933	MS.gene012889:CDS
GTTTGTGCAGGTATGTCATC+TGG	0.494479	3.3:+85000530	None:intergenic
TGGTTTACTCGGTCATATTG+AGG	0.496082	3.3:-84999973	MS.gene012889:CDS
AGAAAATGGTGTGCCAAGCC+AGG	0.504282	3.3:-85000273	MS.gene012889:CDS
TGTTCCATCATCAGATTCCA+TGG	0.504402	3.3:+84999929	None:intergenic
CAAGTGCACGACATTCCAAC+AGG	0.508949	3.3:-85000221	MS.gene012889:CDS
TCGTGCACTTGAACCCAAAA+CGG	0.510201	3.3:+85000233	None:intergenic
ACCTTTGACAGGGTTCCAAA+AGG	0.510921	3.3:+85000424	None:intergenic
GCACAAATTGGAGGTTTACC+TGG	0.512024	3.3:+85000255	None:intergenic
TGAGGGTCTGTGGTTTGTGC+AGG	0.512257	3.3:+85000518	None:intergenic
TGTTCTTGGCGTCATATTCG+AGG	0.521779	3.3:+85000147	None:intergenic
TATGAAGATCAGAGTCCTGA+TGG	0.526288	3.3:-85000102	MS.gene012889:CDS
GTGGCTTAGTAACATCCATC+AGG	0.529918	3.3:+85000087	None:intergenic
ATCATCATGTAGGATCGTAT+TGG	0.531587	3.3:+85000461	None:intergenic
CCCAAAACGGCACAAATTGG+AGG	0.534423	3.3:+85000246	None:intergenic
CAAATTCAAGAGGTAGAACC+AGG	0.536479	3.3:-85000398	MS.gene012889:CDS
CACTCAACGAGAGATGATGA+AGG	0.539546	3.3:+85000579	None:intergenic
AATACGATCCTACATGATGA+TGG	0.539617	3.3:-85000459	MS.gene012889:CDS
GTTACAGTTTCCAGCAGCAA+AGG	0.545564	3.3:-84999831	None:intergenic
CGATTCAAGTACGAGCGGCT+AGG	0.549014	3.3:-85000014	MS.gene012889:CDS
ATGTAGCTGCATTCACCTCC+CGG	0.549990	3.3:+85000038	None:intergenic
CTGGAAACTGTAACTGTCAC+CGG	0.551548	3.3:+84999840	None:intergenic
GGCCAACAAGACGGAGGTGA+GGG	0.558506	3.3:+85000501	None:intergenic
TCACCGGTGGTTCTTGCTGC+CGG	0.563528	3.3:+84999856	None:intergenic
AATACCATGGACCTTTCTTG+AGG	0.563747	3.3:+85000330	None:intergenic
GGACATCAAGGATCAAAAGA+TGG	0.567659	3.3:+85000300	None:intergenic
CGGCCAACAAGACGGAGGTG+AGG	0.572004	3.3:+85000500	None:intergenic
AAGACGGAGGTGAGGGTCTG+TGG	0.573089	3.3:+85000508	None:intergenic
CTACAGAGAATCCTCAAGAA+AGG	0.577250	3.3:-85000341	MS.gene012889:CDS
TTGGCCGGTTAGTGAGGAAG+CGG	0.583738	3.3:+85000480	None:intergenic
ATCGTATTGGCCGGTTAGTG+AGG	0.590416	3.3:+85000474	None:intergenic
TCATCTCTCGTTGAGTGAAG+AGG	0.605479	3.3:-85000573	MS.gene012889:CDS
TCATGTAGGATCGTATTGGC+CGG	0.608829	3.3:+85000465	None:intergenic
CTCTGATCTTCATATATGAG+CGG	0.614510	3.3:+85000111	None:intergenic
GGATTCTCTGTAGGTCTAGG+TGG	0.617802	3.3:+85000351	None:intergenic
GTGCAGGTATGTCATCTGGT+AGG	0.621427	3.3:+85000534	None:intergenic
GAATCCTCAAGAAAGGTCCA+TGG	0.624128	3.3:-85000334	MS.gene012889:CDS
CAAAGGTGTCCAAATTCAAG+AGG	0.624142	3.3:-85000408	MS.gene012889:CDS
TGAGGAAGCGGCCAACAAGA+CGG	0.626222	3.3:+85000492	None:intergenic
TCGCCGGCAGCAAGAACCAC+CGG	0.628520	3.3:-84999859	MS.gene012889:CDS
GAATTTGGACACCTTTGACA+GGG	0.633819	3.3:+85000414	None:intergenic
AAAGTCAGAGAACAACTCGC+CGG	0.642877	3.3:-84999875	MS.gene012889:CDS
ACTCAACGAGAGATGATGAA+GGG	0.644189	3.3:+85000580	None:intergenic
AGATGGTTATCGAAATACCA+TGG	0.644305	3.3:+85000317	None:intergenic
TCTCTCGTTGAGTGAAGAGG+AGG	0.649737	3.3:-85000570	MS.gene012889:CDS
AATTGAAACGTATATAGACC+TGG	0.663195	3.3:+85000380	None:intergenic
ACATTCGATTCAAGTACGAG+CGG	0.719589	3.3:-85000019	MS.gene012889:CDS
GAAACTGTAACTGTCACCGG+TGG	0.726031	3.3:+84999843	None:intergenic
GGAAGCGGCCAACAAGACGG+AGG	0.736655	3.3:+85000495	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAAAAAAGATAAAGAAGC+CGG	-	chr3.3:85000362-85000381	MS.gene012889:CDS	25.0%
	AAGGAAATTGCAAACTTCAT+TGG	-	chr3.3:85000243-85000262	MS.gene012889:CDS	30.0%
	AATTGAAACGTATATAGACC+TGG	+	chr3.3:85000042-85000061	None:intergenic	30.0%
	CAAAAAAAGATAAAGAAGCC+GGG	-	chr3.3:85000363-85000382	MS.gene012889:CDS	30.0%
	GTCTTATGTCAGAAAAAGTA+GGG	-	chr3.3:85000220-85000239	MS.gene012889:CDS	30.0%
!	AATCTTTTCCATCATCATGT+AGG	+	chr3.3:84999971-84999990	None:intergenic	30.0%
!	TGGAAAAGATTGAAACCTTT+TGG	-	chr3.3:84999980-84999999	MS.gene012889:CDS	30.0%
!!	TTGTGAAAAGCTTTATTCCA+TGG	-	chr3.3:85000473-85000492	MS.gene012889:CDS	30.0%
	AATACGATCCTACATGATGA+TGG	-	chr3.3:84999960-84999979	MS.gene012889:CDS	35.0%
	AGATGGTTATCGAAATACCA+TGG	+	chr3.3:85000105-85000124	None:intergenic	35.0%
	ATCATCATGTAGGATCGTAT+TGG	+	chr3.3:84999961-84999980	None:intergenic	35.0%
	CTCTGATCTTCATATATGAG+CGG	+	chr3.3:85000311-85000330	None:intergenic	35.0%
	TATGTCAGAAAAAGTAGGGA+AGG	-	chr3.3:85000224-85000243	MS.gene012889:CDS	35.0%
	TATTCCATGGAATCTGATGA+TGG	-	chr3.3:85000486-85000505	MS.gene012889:CDS	35.0%
!	GGTCTTATGTCAGAAAAAGT+AGG	-	chr3.3:85000219-85000238	MS.gene012889:CDS	35.0%
!	TTTTCTGACATAAGACCTGT+TGG	+	chr3.3:85000216-85000235	None:intergenic	35.0%
!!	GAGATAATTCGAGTTGTTCT+TGG	+	chr3.3:85000289-85000308	None:intergenic	35.0%
!!!	ATCTTTTTTTGACGCTTCAG+TGG	+	chr3.3:85000354-85000373	None:intergenic	35.0%
!!!	GGTAATTTCTGCTACTTTTG+TGG	-	chr3.3:85000426-85000445	MS.gene012889:CDS	35.0%
!!!	TGCTACTTTTGTGGTTTACT+CGG	-	chr3.3:85000435-85000454	MS.gene012889:CDS	35.0%
	AAAAAGATAAAGAAGCCGGG+AGG	-	chr3.3:85000366-85000385	MS.gene012889:CDS	40.0%
	AATACCATGGACCTTTCTTG+AGG	+	chr3.3:85000092-85000111	None:intergenic	40.0%
	ACATTCGATTCAAGTACGAG+CGG	-	chr3.3:85000400-85000419	MS.gene012889:CDS	40.0%
	ACTCAACGAGAGATGATGAA+GGG	+	chr3.3:84999842-84999861	None:intergenic	40.0%
	CAAATTCAAGAGGTAGAACC+AGG	-	chr3.3:85000021-85000040	MS.gene012889:CDS	40.0%
	CTACAGAGAATCCTCAAGAA+AGG	-	chr3.3:85000078-85000097	MS.gene012889:CDS	40.0%
	CTTGATGTCCTTCCAGAAAA+TGG	-	chr3.3:85000132-85000151	MS.gene012889:CDS	40.0%
	CTTTCTTGAGGATTCTCTGT+AGG	+	chr3.3:85000080-85000099	None:intergenic	40.0%
	GAATTTGGACACCTTTGACA+GGG	+	chr3.3:85000008-85000027	None:intergenic	40.0%
	GGACATCAAGGATCAAAAGA+TGG	+	chr3.3:85000122-85000141	None:intergenic	40.0%
	TATGAAGATCAGAGTCCTGA+TGG	-	chr3.3:85000317-85000336	MS.gene012889:CDS	40.0%
	TGAATTTGGACACCTTTGAC+AGG	+	chr3.3:85000009-85000028	None:intergenic	40.0%
	TGGTTTACTCGGTCATATTG+AGG	-	chr3.3:85000446-85000465	MS.gene012889:CDS	40.0%
	TGTTCCATCATCAGATTCCA+TGG	+	chr3.3:85000493-85000512	None:intergenic	40.0%
!!	CAAAGGTGTCCAAATTCAAG+AGG	-	chr3.3:85000011-85000030	MS.gene012889:CDS	40.0%
!!	CATTTTCTGGAAGGACATCA+AGG	+	chr3.3:85000134-85000153	None:intergenic	40.0%
!!	CTGATGATGGAACAAGACTA+TGG	-	chr3.3:85000499-85000518	MS.gene012889:CDS	40.0%
!!	CTGGTTCTACCTCTTGAATT+TGG	+	chr3.3:85000023-85000042	None:intergenic	40.0%
	AAAGTCAGAGAACAACTCGC+CGG	-	chr3.3:85000544-85000563	MS.gene012889:CDS	45.0%
	AATTGGAGGTTTACCTGGCT+TGG	+	chr3.3:85000162-85000181	None:intergenic	45.0%
	ACCTTTGACAGGGTTCCAAA+AGG	+	chr3.3:84999998-85000017	None:intergenic	45.0%
	CACTCAACGAGAGATGATGA+AGG	+	chr3.3:84999843-84999862	None:intergenic	45.0%
	CTGGAAACTGTAACTGTCAC+CGG	+	chr3.3:85000582-85000601	None:intergenic	45.0%
	GAACCCAAAACGGCACAAAT+TGG	+	chr3.3:85000179-85000198	None:intergenic	45.0%
	GAATCCTCAAGAAAGGTCCA+TGG	-	chr3.3:85000085-85000104	MS.gene012889:CDS	45.0%
	GCACAAATTGGAGGTTTACC+TGG	+	chr3.3:85000167-85000186	None:intergenic	45.0%
	GTGGCTTAGTAACATCCATC+AGG	+	chr3.3:85000335-85000354	None:intergenic	45.0%
	GTTTGTGCAGGTATGTCATC+TGG	+	chr3.3:84999892-84999911	None:intergenic	45.0%
	TCATCTCTCGTTGAGTGAAG+AGG	-	chr3.3:84999846-84999865	MS.gene012889:CDS	45.0%
	TCGTGCACTTGAACCCAAAA+CGG	+	chr3.3:85000189-85000208	None:intergenic	45.0%
	TGAGGATTCTCTGTAGGTCT+AGG	+	chr3.3:85000074-85000093	None:intergenic	45.0%
	TGTTCTTGGCGTCATATTCG+AGG	+	chr3.3:85000275-85000294	None:intergenic	45.0%
!	TCATGTAGGATCGTATTGGC+CGG	+	chr3.3:84999957-84999976	None:intergenic	45.0%
!	TTGGCACACCATTTTCTGGA+AGG	+	chr3.3:85000143-85000162	None:intergenic	45.0%
!!!	ACCTCCAATTTGTGCCGTTT+TGG	-	chr3.3:85000172-85000191	MS.gene012889:CDS	45.0%
!!!	ACCTTTTGGAACCCTGTCAA+AGG	-	chr3.3:84999994-85000013	MS.gene012889:CDS	45.0%
!!!	CCTCCAATTTGTGCCGTTTT+GGG	-	chr3.3:85000173-85000192	MS.gene012889:CDS	45.0%
	AGAAAATGGTGTGCCAAGCC+AGG	-	chr3.3:85000146-85000165	MS.gene012889:CDS	50.0%
	ATGTAGCTGCATTCACCTCC+CGG	+	chr3.3:85000384-85000403	None:intergenic	50.0%
	CAAGTGCACGACATTCCAAC+AGG	-	chr3.3:85000198-85000217	MS.gene012889:CDS	50.0%
	CCCAAAACGGCACAAATTGG+AGG	+	chr3.3:85000176-85000195	None:intergenic	50.0%
	GAAACTGTAACTGTCACCGG+TGG	+	chr3.3:85000579-85000598	None:intergenic	50.0%
	GGATTCTCTGTAGGTCTAGG+TGG	+	chr3.3:85000071-85000090	None:intergenic	50.0%
	GTGCAGGTATGTCATCTGGT+AGG	+	chr3.3:84999888-84999907	None:intergenic	50.0%
	TCTCTCGTTGAGTGAAGAGG+AGG	-	chr3.3:84999849-84999868	MS.gene012889:CDS	50.0%
!	ATCGTATTGGCCGGTTAGTG+AGG	+	chr3.3:84999948-84999967	None:intergenic	50.0%
!!	TGGCTTGGCACACCATTTTC+TGG	+	chr3.3:85000147-85000166	None:intergenic	50.0%
	CGATTCAAGTACGAGCGGCT+AGG	-	chr3.3:85000405-85000424	MS.gene012889:CDS	55.0%
	TGAGGAAGCGGCCAACAAGA+CGG	+	chr3.3:84999930-84999949	None:intergenic	55.0%
	TTGGCCGCTTCCTCACTAAC+CGG	-	chr3.3:84999935-84999954	MS.gene012889:CDS	55.0%
	TTGGCCGGTTAGTGAGGAAG+CGG	+	chr3.3:84999942-84999961	None:intergenic	55.0%
!	TGAGGGTCTGTGGTTTGTGC+AGG	+	chr3.3:84999904-84999923	None:intergenic	55.0%
	AAGACGGAGGTGAGGGTCTG+TGG	+	chr3.3:84999914-84999933	None:intergenic	60.0%
	GACCCTCACCTCCGTCTTGT+TGG	-	chr3.3:84999916-84999935	MS.gene012889:CDS	60.0%
	GGCCAACAAGACGGAGGTGA+GGG	+	chr3.3:84999921-84999940	None:intergenic	60.0%
	TCACCGGTGGTTCTTGCTGC+CGG	+	chr3.3:85000566-85000585	None:intergenic	60.0%
	CGGCCAACAAGACGGAGGTG+AGG	+	chr3.3:84999922-84999941	None:intergenic	65.0%
	GGAAGCGGCCAACAAGACGG+AGG	+	chr3.3:84999927-84999946	None:intergenic	65.0%
	TCGCCGGCAGCAAGAACCAC+CGG	-	chr3.3:85000560-85000579	MS.gene012889:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	84999832	85000609	84999832	ID=MS.gene012889
chr3.3	mRNA	84999832	85000609	84999832	ID=MS.gene012889.t1;Parent=MS.gene012889
chr3.3	exon	84999832	85000609	84999832	ID=MS.gene012889.t1.exon1;Parent=MS.gene012889.t1
chr3.3	CDS	84999832	85000609	84999832	ID=cds.MS.gene012889.t1;Parent=MS.gene012889.t1

Gene Sequence

>MS.gene012889

ATGGCATCCCTTCATCATCTCTCGTTGAGTGAAGAGGAGGATGAACTTGAATTCCTACCAGATGACATACCTGCACAAACCACAGACCCTCACCTCCGTCTTGTTGGCCGCTTCCTCACTAACCGGCCAATACGATCCTACATGATGATGGAAAAGATTGAAACCTTTTGGAACCCTGTCAAAGGTGTCCAAATTCAAGAGGTAGAACCAGGTCTATATACGTTTCAATTCTTTCACCACCTAGACCTACAGAGAATCCTCAAGAAAGGTCCATGGTATTTCGATAACCATCTTTTGATCCTTGATGTCCTTCCAGAAAATGGTGTGCCAAGCCAGGTAAACCTCCAATTTGTGCCGTTTTGGGTTCAAGTGCACGACATTCCAACAGGTCTTATGTCAGAAAAAGTAGGGAAGGAAATTGCAAACTTCATTGGCGAATTCCTCGAATATGACGCCAAGAACAACTCGAATTATCTCCGCTCATATATGAAGATCAGAGTCCTGATGGATGTTACTAAGCCACTGAAGCGTCAAAAAAAGATAAAGAAGCCGGGAGGTGAATGCAGCTACATTCGATTCAAGTACGAGCGGCTAGGTAATTTCTGCTACTTTTGTGGTTTACTCGGTCATATTGAGGATTATTGTGAAAAGCTTTATTCCATGGAATCTGATGATGGAACAAGACTATGGAGTTCTGAACTGAGAGTCGAAAAAAGTCAGAGAACAACTCGCCGGCAGCAAGAACCACCGGTGACAGTTACAGTTTCCAGCAGCAAAG

Protein sequence

>MS.gene012889.t1

MASLHHLSLSEEEDELEFLPDDIPAQTTDPHLRLVGRFLTNRPIRSYMMMEKIETFWNPVKGVQIQEVEPGLYTFQFFHHLDLQRILKKGPWYFDNHLLILDVLPENGVPSQVNLQFVPFWVQVHDIPTGLMSEKVGKEIANFIGEFLEYDAKNNSNYLRSYMKIRVLMDVTKPLKRQKKIKKPGGECSYIRFKYERLGNFCYFCGLLGHIEDYCEKLYSMESDDGTRLWSSELRVEKSQRTTRRQQEPPVTVTVSSSK