AlfalfaGEDB | MS.gene61499

M. sativa cultivar XinJiangDaYe / MS.gene61499

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61499.t1	XP_024630779.1	65.7	344	110	2	1	341	1	339	1.00E-126	463.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61499.t1	A0A396JIW9	61.5	379	135	5	1	370	1	377	2.3e-120	441.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene43924	MS.gene61499	PPI
MS.gene046089	MS.gene61499	PPI
MS.gene047954	MS.gene61499	PPI
MS.gene35175	MS.gene61499	PPI
MS.gene77990	MS.gene61499	PPI
MS.gene036423	MS.gene61499	PPI
MS.gene069786	MS.gene61499	PPI
MS.gene054652	MS.gene61499	PPI
MS.gene61499	MS.gene03149	PPI
MS.gene013644	MS.gene61499	PPI
MS.gene013890	MS.gene61499	PPI
MS.gene72520	MS.gene61499	PPI
MS.gene61651	MS.gene61499	PPI
MS.gene66651	MS.gene61499	PPI
MS.gene96109	MS.gene61499	PPI
MS.gene35202	MS.gene61499	PPI
MS.gene051449	MS.gene61499	PPI
MS.gene015093	MS.gene61499	PPI
MS.gene007022	MS.gene61499	PPI
MS.gene63743	MS.gene61499	PPI
MS.gene48477	MS.gene61499	PPI
MS.gene045875	MS.gene61499	PPI
MS.gene038394	MS.gene61499	PPI
MS.gene30136	MS.gene61499	PPI
MS.gene95960	MS.gene61499	PPI
MS.gene008861	MS.gene61499	PPI
MS.gene61452	MS.gene61499	PPI
MS.gene73261	MS.gene61499	PPI
MS.gene024297	MS.gene61499	PPI
MS.gene016102	MS.gene61499	PPI
MS.gene46941	MS.gene61499	PPI
MS.gene96541	MS.gene61499	PPI
MS.gene45083	MS.gene61499	PPI
MS.gene000634	MS.gene61499	PPI
MS.gene85295	MS.gene61499	PPI
MS.gene60390	MS.gene61499	PPI
MS.gene47792	MS.gene61499	PPI
MS.gene46948	MS.gene61499	PPI
MS.gene98471	MS.gene61499	PPI
MS.gene025873	MS.gene61499	PPI
MS.gene012019	MS.gene61499	PPI
MS.gene025401	MS.gene61499	PPI
MS.gene05358	MS.gene61499	PPI
MS.gene93396	MS.gene61499	PPI
MS.gene074257	MS.gene61499	PPI
MS.gene05231	MS.gene61499	PPI
MS.gene010656	MS.gene61499	PPI
MS.gene000629	MS.gene61499	PPI
MS.gene059269	MS.gene61499	PPI
MS.gene40469	MS.gene61499	PPI
MS.gene041264	MS.gene61499	PPI
MS.gene41979	MS.gene61499	PPI
MS.gene024522	MS.gene61499	PPI
MS.gene05319	MS.gene61499	PPI
MS.gene88393	MS.gene61499	PPI
MS.gene56991	MS.gene61499	PPI
MS.gene050679	MS.gene61499	PPI
MS.gene61499	MS.gene21066	PPI
MS.gene85538	MS.gene61499	PPI
MS.gene20246	MS.gene61499	PPI
MS.gene055223	MS.gene61499	PPI
MS.gene28545	MS.gene61499	PPI
MS.gene010290	MS.gene61499	PPI
MS.gene053747	MS.gene61499	PPI
MS.gene65471	MS.gene61499	PPI
MS.gene061850	MS.gene61499	PPI
MS.gene048088	MS.gene61499	PPI
MS.gene008658	MS.gene61499	PPI
MS.gene018474	MS.gene61499	PPI
MS.gene61499	MS.gene86868	PPI
MS.gene003264	MS.gene61499	PPI
MS.gene073582	MS.gene61499	PPI
MS.gene058889	MS.gene61499	PPI
MS.gene98616	MS.gene61499	PPI
MS.gene40828	MS.gene61499	PPI
MS.gene53904	MS.gene61499	PPI
MS.gene08708	MS.gene61499	PPI
MS.gene09517	MS.gene61499	PPI
MS.gene69586	MS.gene61499	PPI
MS.gene042027	MS.gene61499	PPI
MS.gene69490	MS.gene61499	PPI
MS.gene043140	MS.gene61499	PPI
MS.gene015248	MS.gene61499	PPI
MS.gene84790	MS.gene61499	PPI
MS.gene80362	MS.gene61499	PPI
MS.gene44083	MS.gene61499	PPI
MS.gene37537	MS.gene61499	PPI
MS.gene013193	MS.gene61499	PPI
MS.gene04970	MS.gene61499	PPI
MS.gene08106	MS.gene61499	PPI
MS.gene24278	MS.gene61499	PPI
MS.gene025158	MS.gene61499	PPI
MS.gene027994	MS.gene61499	PPI
MS.gene068629	MS.gene61499	PPI
MS.gene019001	MS.gene61499	PPI
MS.gene96163	MS.gene61499	PPI
MS.gene43393	MS.gene61499	PPI
MS.gene08713	MS.gene61499	PPI
MS.gene23521	MS.gene61499	PPI
MS.gene63150	MS.gene61499	PPI
MS.gene54051	MS.gene61499	PPI
MS.gene98127	MS.gene61499	PPI
MS.gene03149	MS.gene61499	PPI
MS.gene87529	MS.gene61499	PPI
MS.gene024405	MS.gene61499	PPI
MS.gene012748	MS.gene61499	PPI
MS.gene04134	MS.gene61499	PPI
MS.gene83979	MS.gene61499	PPI
MS.gene062067	MS.gene61499	PPI
MS.gene92517	MS.gene61499	PPI
MS.gene057934	MS.gene61499	PPI
MS.gene57044	MS.gene61499	PPI
MS.gene054508	MS.gene61499	PPI
MS.gene89815	MS.gene61499	PPI
MS.gene028714	MS.gene61499	PPI
MS.gene049610	MS.gene61499	PPI
MS.gene017586	MS.gene61499	PPI
MS.gene038676	MS.gene61499	PPI
MS.gene060718	MS.gene61499	PPI
MS.gene98756	MS.gene61499	PPI
MS.gene71637	MS.gene61499	PPI
MS.gene042651	MS.gene61499	PPI
MS.gene61499	MS.gene022929	PPI
MS.gene05576	MS.gene61499	PPI
MS.gene61499	MS.gene007022	PPI
MS.gene52349	MS.gene61499	PPI
MS.gene98467	MS.gene61499	PPI
MS.gene46353	MS.gene61499	PPI
MS.gene42010	MS.gene61499	PPI
MS.gene037049	MS.gene61499	PPI
MS.gene034643	MS.gene61499	PPI
MS.gene037634	MS.gene61499	PPI
MS.gene61499	MS.gene048342	PPI
MS.gene000379	MS.gene61499	PPI
MS.gene048342	MS.gene61499	PPI
MS.gene64130	MS.gene61499	PPI
MS.gene24070	MS.gene61499	PPI
MS.gene60266	MS.gene61499	PPI
MS.gene016719	MS.gene61499	PPI
MS.gene071784	MS.gene61499	PPI
MS.gene61499	MS.gene88393	PPI
MS.gene061579	MS.gene61499	PPI
MS.gene039692	MS.gene61499	PPI
MS.gene76420	MS.gene61499	PPI
MS.gene071320	MS.gene61499	PPI
MS.gene022929	MS.gene61499	PPI
MS.gene61499	MS.gene008658	PPI
MS.gene81712	MS.gene61499	PPI
MS.gene026680	MS.gene61499	PPI
MS.gene26433	MS.gene61499	PPI
MS.gene34463	MS.gene61499	PPI
MS.gene22730	MS.gene61499	PPI
MS.gene042559	MS.gene61499	PPI
MS.gene22200	MS.gene61499	PPI
MS.gene071133	MS.gene61499	PPI
MS.gene055546	MS.gene61499	PPI
MS.gene26038	MS.gene61499	PPI
MS.gene08606	MS.gene61499	PPI
MS.gene85308	MS.gene61499	PPI
MS.gene49057	MS.gene61499	PPI
MS.gene073573	MS.gene61499	PPI
MS.gene61499	MS.gene08606	PPI
MS.gene012889	MS.gene61499	PPI
MS.gene031967	MS.gene61499	PPI
MS.gene053373	MS.gene61499	PPI
MS.gene34812	MS.gene61499	PPI
MS.gene61499	MS.gene000640	PPI
MS.gene02896	MS.gene61499	PPI
MS.gene063214	MS.gene61499	PPI
MS.gene55682	MS.gene61499	PPI
MS.gene51799	MS.gene61499	PPI
MS.gene58419	MS.gene61499	PPI
MS.gene61499	MS.gene74639	PPI
MS.gene61499	MS.gene071133	PPI
MS.gene067340	MS.gene61499	PPI
MS.gene65623	MS.gene61499	PPI
MS.gene65474	MS.gene61499	PPI
MS.gene019177	MS.gene61499	PPI
MS.gene69495	MS.gene61499	PPI
MS.gene049512	MS.gene61499	PPI
MS.gene29406	MS.gene61499	PPI
MS.gene61499	MS.gene37955	PPI
MS.gene038623	MS.gene61499	PPI
MS.gene86275	MS.gene61499	PPI
MS.gene05357	MS.gene61499	PPI
MS.gene032311	MS.gene61499	PPI
MS.gene61499	MS.gene84790	PPI
MS.gene004417	MS.gene61499	PPI
MS.gene86849	MS.gene61499	PPI
MS.gene50392	MS.gene61499	PPI
MS.gene37955	MS.gene61499	PPI
MS.gene072852	MS.gene61499	PPI
MS.gene71675	MS.gene61499	PPI
MS.gene41393	MS.gene61499	PPI
MS.gene39956	MS.gene61499	PPI
MS.gene021968	MS.gene61499	PPI
MS.gene09589	MS.gene61499	PPI
MS.gene61499	MS.gene027994	PPI
MS.gene44144	MS.gene61499	PPI
MS.gene057828	MS.gene61499	PPI
MS.gene34197	MS.gene61499	PPI
MS.gene044729	MS.gene61499	PPI
MS.gene66650	MS.gene61499	PPI
MS.gene005120	MS.gene61499	PPI
MS.gene86868	MS.gene61499	PPI
MS.gene048072	MS.gene61499	PPI
MS.gene060386	MS.gene61499	PPI
MS.gene71588	MS.gene61499	PPI
MS.gene61499	MS.gene61651	PPI
MS.gene04749	MS.gene61499	PPI
MS.gene05436	MS.gene61499	PPI
MS.gene059736	MS.gene61499	PPI
MS.gene70932	MS.gene61499	PPI
MS.gene61499	MS.gene025987	PPI
MS.gene027169	MS.gene61499	PPI
MS.gene62249	MS.gene61499	PPI
MS.gene61499	MS.gene043140	PPI
MS.gene81194	MS.gene61499	PPI
MS.gene025675	MS.gene61499	PPI
MS.gene42903	MS.gene61499	PPI
MS.gene021965	MS.gene61499	PPI
MS.gene055683	MS.gene61499	PPI
MS.gene042614	MS.gene61499	PPI
MS.gene41643	MS.gene61499	PPI
MS.gene26844	MS.gene61499	PPI
MS.gene021555	MS.gene61499	PPI
MS.gene060773	MS.gene61499	PPI
MS.gene048747	MS.gene61499	PPI
MS.gene021647	MS.gene61499	PPI
MS.gene69584	MS.gene61499	PPI
MS.gene019165	MS.gene61499	PPI
MS.gene048706	MS.gene61499	PPI
MS.gene61499	MS.gene015093	PPI
MS.gene61499	MS.gene24278	PPI
MS.gene61499	MS.gene000379	PPI
MS.gene009121	MS.gene61499	PPI
MS.gene61499	MS.gene59460	PPI
MS.gene94654	MS.gene61499	PPI
MS.gene024841	MS.gene61499	PPI
MS.gene51804	MS.gene61499	PPI
MS.gene87845	MS.gene61499	PPI
MS.gene36069	MS.gene61499	PPI
MS.gene97410	MS.gene61499	PPI
MS.gene61499	MS.gene09517	PPI
MS.gene83772	MS.gene61499	PPI
MS.gene32415	MS.gene61499	PPI
MS.gene009213	MS.gene61499	PPI
MS.gene060788	MS.gene61499	PPI
MS.gene035626	MS.gene61499	PPI
MS.gene008927	MS.gene61499	PPI
MS.gene045614	MS.gene61499	PPI
MS.gene049561	MS.gene61499	PPI
MS.gene009781	MS.gene61499	PPI
MS.gene61499	MS.gene62249	PPI
MS.gene63762	MS.gene61499	PPI
MS.gene27329	MS.gene61499	PPI
MS.gene30208	MS.gene61499	PPI
MS.gene028716	MS.gene61499	PPI
MS.gene21066	MS.gene61499	PPI
MS.gene06164	MS.gene61499	PPI
MS.gene013676	MS.gene61499	PPI
MS.gene55209	MS.gene61499	PPI
MS.gene61499	MS.gene44144	PPI
MS.gene64178	MS.gene61499	PPI
MS.gene030090	MS.gene61499	PPI
MS.gene95561	MS.gene61499	PPI
MS.gene96174	MS.gene61499	PPI
MS.gene018196	MS.gene61499	PPI
MS.gene61499	MS.gene60390	PPI
MS.gene065627	MS.gene61499	PPI
MS.gene57019	MS.gene61499	PPI
MS.gene010890	MS.gene61499	PPI
MS.gene018745	MS.gene61499	PPI
MS.gene65546	MS.gene61499	PPI
MS.gene09023	MS.gene61499	PPI
MS.gene000506	MS.gene61499	PPI
MS.gene74639	MS.gene61499	PPI
MS.gene032299	MS.gene61499	PPI
MS.gene03936	MS.gene61499	PPI
MS.gene038098	MS.gene61499	PPI
MS.gene60059	MS.gene61499	PPI
MS.gene062511	MS.gene61499	PPI
MS.gene22111	MS.gene61499	PPI
MS.gene28718	MS.gene61499	PPI
MS.gene055560	MS.gene61499	PPI
MS.gene008275	MS.gene61499	PPI
MS.gene29321	MS.gene61499	PPI
MS.gene61499	MS.gene025158	PPI
MS.gene34618	MS.gene61499	PPI
MS.gene59460	MS.gene61499	PPI
MS.gene038757	MS.gene61499	PPI
MS.gene064966	MS.gene61499	PPI
MS.gene069624	MS.gene61499	PPI
MS.gene016632	MS.gene61499	PPI
MS.gene002590	MS.gene61499	PPI
MS.gene025987	MS.gene61499	PPI
MS.gene61499	MS.gene018474	PPI
MS.gene000640	MS.gene61499	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61499.t1	MTR_0010s0370	75.556	180	44	0	1	180	1	180	2.57e-98	290
MS.gene61499.t1	MTR_0010s0350	75.556	180	44	0	1	180	1	180	2.57e-98	290
MS.gene61499.t1	MTR_1g036720	71.171	111	32	0	1	111	1	111	3.09e-52	169
MS.gene61499.t1	MTR_1g036710	46.269	201	64	3	132	329	1	160	3.04e-48	166
MS.gene61499.t1	MTR_1g051330	65.152	132	46	0	248	379	20	151	7.83e-45	164
MS.gene61499.t1	MTR_6g033215	33.708	178	114	2	24	199	119	294	3.26e-30	117
MS.gene61499.t1	MTR_7g089990	45.370	108	57	1	104	209	86	193	6.44e-22	93.2
MS.gene61499.t1	MTR_3g053920	32.374	139	77	2	33	171	35	156	4.68e-19	86.7
MS.gene61499.t1	MTR_4g103530	38.202	89	55	0	57	145	5	93	4.54e-16	76.3
MS.gene61499.t1	MTR_2g061500	35.106	94	61	0	34	127	36	129	1.52e-14	70.5
MS.gene61499.t1	MTR_7g106180	28.302	159	104	3	20	175	15	166	4.32e-14	71.6
MS.gene61499.t1	MTR_3g024240	33.884	121	78	1	82	200	55	175	3.72e-13	67.8
MS.gene61499.t1	MTR_8g468010	39.062	64	38	1	120	182	6	69	2.37e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61499.t1	AT3G31430	28.671	143	98	1	73	215	173	311	2.24e-19	88.6
MS.gene61499.t1	AT5G18636	25.157	159	117	1	57	215	62	218	4.81e-17	82.0
MS.gene61499.t1	AT5G25200	25.157	159	117	1	57	215	62	218	6.02e-17	82.0
MS.gene61499.t1	AT2G41590	26.415	159	115	1	57	215	62	218	6.37e-17	81.6
MS.gene61499.t1	AT2G17920	27.160	162	110	3	57	215	62	218	1.24e-16	80.1
MS.gene61499.t1	AT5G36228	21.973	223	172	1	3	225	8	228	1.36e-16	80.9
MS.gene61499.t1	AT2G02103	24.528	159	118	1	57	215	62	218	1.46e-16	80.9
MS.gene61499.t1	AT2G13450	22.703	185	141	1	31	215	36	218	1.27e-15	78.2
MS.gene61499.t1	AT3G57587	25.882	170	118	3	49	215	54	218	1.58e-15	77.8
MS.gene61499.t1	AT3G47920	28.369	141	99	2	75	215	9	147	1.72e-14	73.9
MS.gene61499.t1	AT4G02000	25.185	135	99	1	81	215	6	138	7.10e-14	72.4
MS.gene61499.t1	AT5G36225	27.778	90	65	0	121	210	17	106	4.56e-11	62.8

Find 95 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCTTCAATTTGCCCCATTC+TGG	0.182081	8.3:-13250391	MS.gene61499:CDS
GATTACGCCATTGTTGATTT+TGG	0.248245	8.3:+13249839	None:intergenic
AGTACCACGGGCCCTTCTTT+AGG	0.279703	8.3:+13250474	None:intergenic
AGGATGATGGCACACGACTA+TGG	0.315161	8.3:-13250064	MS.gene61499:CDS
TGCAGAGATGTTCTCATTGC+AGG	0.317035	8.3:+13249623	None:intergenic
GATCCTCGCCTCTGCCTTGT+TGG	0.331565	8.3:-13250647	MS.gene61499:CDS
ACGGACATTGCGAATTATAT+TGG	0.334451	8.3:-13250320	MS.gene61499:CDS
TTTAAGCCTCTAGAACGTTT+TGG	0.335560	8.3:+13249753	None:intergenic
TGGAAACGATAGAGACCTTC+TGG	0.340204	8.3:-13250583	MS.gene61499:CDS
AATCAATAACTCAGTCAATA+TGG	0.357710	8.3:-13249820	MS.gene61499:CDS
AGTCAATATGGAGCTGTTAC+AGG	0.363513	8.3:-13249808	MS.gene61499:CDS
GGCCCTTCTTTAGGATTCTC+TGG	0.365353	8.3:+13250483	None:intergenic
TTTGTTATTAATTGTATTGA+AGG	0.366911	8.3:+13249716	None:intergenic
GTGTGAGGGCTTCGTCGATC+TGG	0.368163	8.3:+13250678	None:intergenic
CTCGCCGTCAATCAGAATTC+GGG	0.373416	8.3:-13249992	MS.gene61499:CDS
TGCTACTACTGCGGAATGTT+AGG	0.378044	8.3:-13250128	MS.gene61499:CDS
GCTCGCCGTCAATCAGAATT+CGG	0.378069	8.3:-13249993	MS.gene61499:CDS
ATTTGCCCCATTCTGGATAC+AGG	0.379704	8.3:-13250384	MS.gene61499:CDS
TGGTTAGGAGCAATATTCTC+TGG	0.388830	8.3:+13250419	None:intergenic
AATCGAGGTGGTGTGCAAAT+TGG	0.392884	8.3:+13250507	None:intergenic
AATTGAAGGGCAACTTGGTT+AGG	0.399290	8.3:+13250404	None:intergenic
ATCATCATATATGACCGAAT+TGG	0.403084	8.3:+13250605	None:intergenic
CTGGGATCACATTGAGGATC+AGG	0.418784	8.3:+13250438	None:intergenic
TTCACACAAACAAGCCAATT+CGG	0.419848	8.3:-13250619	MS.gene61499:CDS
ATAGGCCTGGCTCTATCTCT+TGG	0.434596	8.3:+13250537	None:intergenic
CATGCACCTGTATCCAGAAT+GGG	0.472692	8.3:+13250378	None:intergenic
TCCAGAATGGGGCAAATTGA+AGG	0.483650	8.3:+13250390	None:intergenic
CCTCATCACTGAGGGAAAGT+TGG	0.485135	8.3:+13250717	None:intergenic
AAAATCCAAGAGATAGAGCC+AGG	0.490259	8.3:-13250542	MS.gene61499:CDS
GTGCAAATTGGAAGGTGAAT+AGG	0.493892	8.3:+13250519	None:intergenic
GGGCAAATTGAAGGGCAACT+TGG	0.494299	8.3:+13250399	None:intergenic
TCATGCACCTGTATCCAGAA+TGG	0.505879	8.3:+13250377	None:intergenic
GGATGATGGCACACGACTAT+GGG	0.511194	8.3:-13250063	MS.gene61499:CDS
CAGCACACGAATTCTCATAT+AGG	0.516239	8.3:+13250250	None:intergenic
CATATAGGAACGGAGGTGGT+TGG	0.516500	8.3:+13250265	None:intergenic
TTGGCTTGTTTGTGTGAAAA+CGG	0.519984	8.3:+13250624	None:intergenic
TATTAATTGTATTGAAGGAC+TGG	0.520039	8.3:+13249721	None:intergenic
AATTCGGTCATATATGATGA+TGG	0.525605	8.3:-13250603	MS.gene61499:CDS
ATGTGCTAAGGCTGCAGAGT+TGG	0.530202	8.3:+13249893	None:intergenic
ATTTCTGCCTCGTTAACTGC+AGG	0.531682	8.3:+13249687	None:intergenic
AATTGGAAGGTGAATAGGCC+TGG	0.533003	8.3:+13250524	None:intergenic
TTTCCAGAGAATCCTAAAGA+AGG	0.533702	8.3:-13250486	MS.gene61499:CDS
GGTTAGGAGCAATATTCTCT+GGG	0.538528	8.3:+13250420	None:intergenic
CATCACTGAGGGAAAGTTGG+TGG	0.544634	8.3:+13250720	None:intergenic
TTGGCTGGACGCATTAATTG+TGG	0.548346	8.3:+13249858	None:intergenic
GCAGCAGTTACAGTTTCAAA+CGG	0.560023	8.3:-13249951	MS.gene61499:CDS
TTACTGCGATAAACTTTACT+CGG	0.568634	8.3:-13250093	MS.gene61499:CDS
GAGGATCATCTGTCTGTGTG+AGG	0.568745	8.3:+13250663	None:intergenic
TAAACTTTACTCGGCTGATG+AGG	0.570794	8.3:-13250084	MS.gene61499:CDS
AGGCTTCATGACAGAGAAGG+TGG	0.570991	8.3:-13250345	MS.gene61499:CDS
AGGATCATCTGTCTGTGTGA+GGG	0.571450	8.3:+13250664	None:intergenic
TTCTCATATAGGAACGGAGG+TGG	0.572631	8.3:+13250261	None:intergenic
TTTGAAACTGTAACTGCTGC+CGG	0.577227	8.3:+13249954	None:intergenic
GGCTTCATGACAGAGAAGGT+GGG	0.577893	8.3:-13250344	MS.gene61499:CDS
TACTCGGCTGATGAGGATGA+TGG	0.578869	8.3:-13250077	MS.gene61499:CDS
AAGTTCAAATATGAGCGTCT+AGG	0.580615	8.3:-13250158	MS.gene61499:CDS
ACCTTCTGGAATCCTGTCAA+AGG	0.580850	8.3:-13250569	MS.gene61499:CDS
TTCGTTCTCCTCATCACTGA+GGG	0.583353	8.3:+13250709	None:intergenic
CAGGTGCATGACATTCCTCT+AGG	0.585034	8.3:-13250365	MS.gene61499:CDS
CCAGAATGGGGCAAATTGAA+GGG	0.587445	8.3:+13250391	None:intergenic
TCGCCGTCAATCAGAATTCG+GGG	0.589042	8.3:-13249991	MS.gene61499:CDS
TTGTGTGAAAACGGCCAACA+AGG	0.590289	8.3:+13250633	None:intergenic
TATCGCAGTAATCTTCTATG+TGG	0.593876	8.3:+13250105	None:intergenic
GGTGATTGTCAAAGTACCAC+GGG	0.595716	8.3:+13250462	None:intergenic
TTCCAGAGAATCCTAAAGAA+GGG	0.596625	8.3:-13250485	MS.gene61499:CDS
TTAGGATTCTCTGGAAATCG+AGG	0.600593	8.3:+13250492	None:intergenic
TATTCTCTGGGATCACATTG+AGG	0.603453	8.3:+13250432	None:intergenic
CGGCCAACAAGGCAGAGGCG+AGG	0.607947	8.3:+13250644	None:intergenic
TCCTTTGACAGGATTCCAGA+AGG	0.610347	8.3:+13250568	None:intergenic
GCTTCATGACAGAGAAGGTG+GGG	0.616633	8.3:-13250343	MS.gene61499:CDS
CGTCCAGCCAAAATCAACAA+TGG	0.619038	8.3:-13249846	MS.gene61499:CDS
GAAAACGGCCAACAAGGCAG+AGG	0.620339	8.3:+13250639	None:intergenic
AACTGCGTGTAGAAAAGCAA+AGG	0.620924	8.3:-13250034	MS.gene61499:CDS
GTTACAGTTTCAAACGGTAA+CGG	0.628501	8.3:-13249945	MS.gene61499:CDS
CACGAATTCTCATATAGGAA+CGG	0.629208	8.3:+13250255	None:intergenic
ATCATCACCTGCAGTTAACG+AGG	0.637893	8.3:-13249694	MS.gene61499:CDS
CCAACTTTCCCTCAGTGATG+AGG	0.637986	8.3:-13250717	MS.gene61499:CDS
AGCAAACCAAAACGTTCTAG+AGG	0.638592	8.3:-13249759	MS.gene61499:CDS
TCTAGGCTTCATGACAGAGA+AGG	0.641763	8.3:-13250348	MS.gene61499:CDS
GGAAACTACTGCTACTACTG+CGG	0.642097	8.3:-13250137	MS.gene61499:CDS
GATGATGGCACACGACTATG+GGG	0.648219	8.3:-13250062	MS.gene61499:CDS
TTCTCTGTCATGAAGCCTAG+AGG	0.648755	8.3:+13250350	None:intergenic
CATGACAGAGAAGGTGGGGA+CGG	0.650231	8.3:-13250339	MS.gene61499:CDS
ACGTTCTAGAGGCTTAAATG+AGG	0.651463	8.3:-13249748	MS.gene61499:CDS
GGATCACATTGAGGATCAGG+AGG	0.653310	8.3:+13250441	None:intergenic
ATTGCGCAGTAAATGTGCTA+AGG	0.668027	8.3:+13249881	None:intergenic
ATGCACCTGTATCCAGAATG+GGG	0.670343	8.3:+13250379	None:intergenic
GGATTCTCTGGAAATCGAGG+TGG	0.674590	8.3:+13250495	None:intergenic
AGGTGATTGTCAAAGTACCA+CGG	0.679925	8.3:+13250461	None:intergenic
CGCCGTCAATCAGAATTCGG+GGG	0.682054	8.3:-13249990	MS.gene61499:CDS
GAATCCTAAAGAAGGGCCCG+TGG	0.693392	8.3:-13250478	MS.gene61499:CDS
GAGGTGGTGTGCAAATTGGA+AGG	0.694439	8.3:+13250511	None:intergenic
ATTCGTTCTCCTCATCACTG+AGG	0.717239	8.3:+13250708	None:intergenic
GAATTCTCATATAGGAACGG+AGG	0.721589	8.3:+13250258	None:intergenic
ATGATGGCACACGACTATGG+GGG	0.726460	8.3:-13250061	MS.gene61499:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGTTATTAATTGTATTGA+AGG	+	chr8.3:13249854-13249873	None:intergenic	15.0%
!!	AAGCAATGAATATAATATCT+TGG	-	chr8.3:13250054-13250073	MS.gene61499:CDS	20.0%
!!!	TAGCAAGTTAATGAAATTTT+GGG	+	chr8.3:13250312-13250331	None:intergenic	20.0%
!!!	TTAGCAAGTTAATGAAATTT+TGG	+	chr8.3:13250313-13250332	None:intergenic	20.0%
!	AATCAATAACTCAGTCAATA+TGG	-	chr8.3:13249747-13249766	MS.gene61499:CDS	25.0%
!	GATATTATATTCATTGCTTC+CGG	+	chr8.3:13250053-13250072	None:intergenic	25.0%
!	TATTAATTGTATTGAAGGAC+TGG	+	chr8.3:13249849-13249868	None:intergenic	25.0%
!!!	TATTTTTGGCATCATATTCA+AGG	+	chr8.3:13249279-13249298	None:intergenic	25.0%
	AAAAAAATCAAGAGACAAGG+TGG	-	chr8.3:13249370-13249389	MS.gene61499:intron	30.0%
	AAAAAATCAAGAGACAAGGT+GGG	-	chr8.3:13249371-13249390	MS.gene61499:intron	30.0%
	AATTCGGTCATATATGATGA+TGG	-	chr8.3:13248964-13248983	MS.gene61499:intron	30.0%
	ATCATCATATATGACCGAAT+TGG	+	chr8.3:13248965-13248984	None:intergenic	30.0%
	CAGAAAAAAATCAAGAGACA+AGG	-	chr8.3:13249367-13249386	MS.gene61499:intron	30.0%
	CTAATTAAAGATCCTGTCAT+TGG	-	chr8.3:13250329-13250348	MS.gene61499:CDS	30.0%
	GTAGAAAAGCAAAGGAAAAA+CGG	-	chr8.3:13249541-13249560	MS.gene61499:intron	30.0%
	TCAATGAGGATTACAATTTC+CGG	+	chr8.3:13250146-13250165	None:intergenic	30.0%
	TGTAATCCTCATTGAAACTT+TGG	-	chr8.3:13250151-13250170	MS.gene61499:CDS	30.0%
	TTACTGCGATAAACTTTACT+CGG	-	chr8.3:13249474-13249493	MS.gene61499:intron	30.0%
	TTATATGATATGCCTCTTCA+TGG	-	chr8.3:13250566-13250585	MS.gene61499:CDS	30.0%
	AAAAATCAAGAGACAAGGTG+GGG	-	chr8.3:13249372-13249391	MS.gene61499:intron	35.0%
	AAGTTCAAATATGAGCGTCT+AGG	-	chr8.3:13249409-13249428	MS.gene61499:intron	35.0%
	AATCAACAGAATTTCGCCAA+AGG	+	chr8.3:13250271-13250290	None:intergenic	35.0%
	ACGGACATTGCGAATTATAT+TGG	-	chr8.3:13249247-13249266	MS.gene61499:intron	35.0%
	CAATTTCCGGTTTATAGACA+CGG	+	chr8.3:13250133-13250152	None:intergenic	35.0%
	CACGAATTCTCATATAGGAA+CGG	+	chr8.3:13249315-13249334	None:intergenic	35.0%
	CATTCACCAAAGTTTCAATG+AGG	+	chr8.3:13250160-13250179	None:intergenic	35.0%
	CTCATTGAAACTTTGGTGAA+TGG	-	chr8.3:13250158-13250177	MS.gene61499:CDS	35.0%
	GTTACAGTTTCAAACGGTAA+CGG	-	chr8.3:13249622-13249641	MS.gene61499:CDS	35.0%
	TATCGCAGTAATCTTCTATG+TGG	+	chr8.3:13249465-13249484	None:intergenic	35.0%
	TCAGACTTATGCTACTCTAT+TGG	-	chr8.3:13250191-13250210	MS.gene61499:CDS	35.0%
	TTCACACAAACAAGCCAATT+CGG	-	chr8.3:13248948-13248967	MS.gene61499:intron	35.0%
	TTCATGGTTACCAATTCACA+TGG	-	chr8.3:13250582-13250601	MS.gene61499:CDS	35.0%
	TTCCAGAGAATCCTAAAGAA+GGG	-	chr8.3:13249082-13249101	MS.gene61499:intron	35.0%
	TTTCCAGAGAATCCTAAAGA+AGG	-	chr8.3:13249081-13249100	MS.gene61499:intron	35.0%
!	GAGGTGGTTGGAATTATTTT+TGG	+	chr8.3:13249293-13249312	None:intergenic	35.0%
!	TCTCATTGCAGGATGATTTT+TGG	+	chr8.3:13249936-13249955	None:intergenic	35.0%
!!	CATTTTTCAGTTGACCGTAT+TGG	-	chr8.3:13250224-13250243	MS.gene61499:CDS	35.0%
!!	TTGGCTTGTTTGTGTGAAAA+CGG	+	chr8.3:13248946-13248965	None:intergenic	35.0%
!!!	ATTTTTTTCTGACGCTTCAC+TGG	+	chr8.3:13249358-13249377	None:intergenic	35.0%
!!!	CTCGGCAACTCATTTTTTAT+CGG	-	chr8.3:13250005-13250024	MS.gene61499:CDS	35.0%
!!!	GATTACGCCATTGTTGATTT+TGG	+	chr8.3:13249731-13249750	None:intergenic	35.0%
!!!	TTTAAGCCTCTAGAACGTTT+TGG	+	chr8.3:13249817-13249836	None:intergenic	35.0%
	AAAATCCAAGAGATAGAGCC+AGG	-	chr8.3:13249025-13249044	MS.gene61499:intron	40.0%
	AACTGCGTGTAGAAAAGCAA+AGG	-	chr8.3:13249533-13249552	MS.gene61499:intron	40.0%
	AATCCTCTAATGAGCCATGA+AGG	+	chr8.3:13250521-13250540	None:intergenic	40.0%
	AATTGAAGGGCAACTTGGTT+AGG	+	chr8.3:13249166-13249185	None:intergenic	40.0%
	AGCAAACCAAAACGTTCTAG+AGG	-	chr8.3:13249808-13249827	MS.gene61499:CDS	40.0%
	AGTCAATATGGAGCTGTTAC+AGG	-	chr8.3:13249759-13249778	MS.gene61499:CDS	40.0%
	ATAATATCTTGGAACTGCCG+CGG	-	chr8.3:13250065-13250084	MS.gene61499:CDS	40.0%
	ATGGCTCATTAGAGGATTTC+AGG	-	chr8.3:13250523-13250542	MS.gene61499:CDS	40.0%
	ATTGCGCAGTAAATGTGCTA+AGG	+	chr8.3:13249689-13249708	None:intergenic	40.0%
	CAGCACACGAATTCTCATAT+AGG	+	chr8.3:13249320-13249339	None:intergenic	40.0%
	GAAAAGCAAAGGAAAAACGG+AGG	-	chr8.3:13249544-13249563	MS.gene61499:intron	40.0%
	GAATTCTCATATAGGAACGG+AGG	+	chr8.3:13249312-13249331	None:intergenic	40.0%
	GCAGCAGTTACAGTTTCAAA+CGG	-	chr8.3:13249616-13249635	MS.gene61499:CDS	40.0%
	GGTTAGGAGCAATATTCTCT+GGG	+	chr8.3:13249150-13249169	None:intergenic	40.0%
	GTGCAAATTGGAAGGTGAAT+AGG	+	chr8.3:13249051-13249070	None:intergenic	40.0%
	TAAACTTTACTCGGCTGATG+AGG	-	chr8.3:13249483-13249502	MS.gene61499:intron	40.0%
	TGCTCTTGAACCATGTGAAT+TGG	+	chr8.3:13250595-13250614	None:intergenic	40.0%
	TGGTTAGGAGCAATATTCTC+TGG	+	chr8.3:13249151-13249170	None:intergenic	40.0%
	TTATATTCATTGCTTCCGGC+AGG	+	chr8.3:13250049-13250068	None:intergenic	40.0%
!	AGGTGATTGTCAAAGTACCA+CGG	+	chr8.3:13249109-13249128	None:intergenic	40.0%
!	CGGACAAGGTCTTTAATTTC+AGG	+	chr8.3:13250113-13250132	None:intergenic	40.0%
!	GTGAATTGGTAACCATGAAG+AGG	+	chr8.3:13250581-13250600	None:intergenic	40.0%
!	TTAGGATTCTCTGGAAATCG+AGG	+	chr8.3:13249078-13249097	None:intergenic	40.0%
!	TTCACATGGTTCAAGAGCAT+CGG	-	chr8.3:13250596-13250615	MS.gene61499:CDS	40.0%
!	TTGACCGTATTGGTAGAAGT+GGG	-	chr8.3:13250234-13250253	MS.gene61499:CDS	40.0%
!	TTTGAAACTGTAACTGCTGC+CGG	+	chr8.3:13249616-13249635	None:intergenic	40.0%
!	TTTTGTCTTCAGAGGAGAGA+TGG	+	chr8.3:13250481-13250500	None:intergenic	40.0%
!!	ACGTTCTAGAGGCTTAAATG+AGG	-	chr8.3:13249819-13249838	MS.gene61499:CDS	40.0%
!!	TATTCTCTGGGATCACATTG+AGG	+	chr8.3:13249138-13249157	None:intergenic	40.0%
!!!	GGATGATTTTTGGTCTGCTA+AGG	+	chr8.3:13249926-13249945	None:intergenic	40.0%
!!!	TCTAGAACGTTTTGGTTTGC+TGG	+	chr8.3:13249809-13249828	None:intergenic	40.0%
!!!	TGGATTTTGACTCCTTTGAC+AGG	+	chr8.3:13249013-13249032	None:intergenic	40.0%
	AAACGCTGCACTCCAAACTT+TGG	-	chr8.3:13250688-13250707	MS.gene61499:CDS	45.0%
	AACGATCCATGGTGCATCAT+TGG	-	chr8.3:13250443-13250462	MS.gene61499:CDS	45.0%
	AAGCAAAGGAAAAACGGAGG+AGG	-	chr8.3:13249547-13249566	MS.gene61499:intron	45.0%
	AAGTCACCAATGATGCACCA+TGG	+	chr8.3:13250452-13250471	None:intergenic	45.0%
	AATCGAGGTGGTGTGCAAAT+TGG	+	chr8.3:13249063-13249082	None:intergenic	45.0%
	AATTGGAAGGTGAATAGGCC+TGG	+	chr8.3:13249046-13249065	None:intergenic	45.0%
	ACGGTCAATACGAGCTTCTT+TGG	+	chr8.3:13250633-13250652	None:intergenic	45.0%
	AGGATCATCTGTCTGTGTGA+GGG	+	chr8.3:13248906-13248925	None:intergenic	45.0%
	ATATGGAGGCCACCAAAGTT+TGG	+	chr8.3:13250703-13250722	None:intergenic	45.0%
	ATCATCACCTGCAGTTAACG+AGG	-	chr8.3:13249873-13249892	MS.gene61499:CDS	45.0%
	ATGCACCTGTATCCAGAATG+GGG	+	chr8.3:13249191-13249210	None:intergenic	45.0%
	ATTCGTTCTCCTCATCACTG+AGG	+	chr8.3:13248862-13248881	None:intergenic	45.0%
	ATTTCTGCCTCGTTAACTGC+AGG	+	chr8.3:13249883-13249902	None:intergenic	45.0%
	ATTTGCCCCATTCTGGATAC+AGG	-	chr8.3:13249183-13249202	MS.gene61499:intron	45.0%
	CATGCACCTGTATCCAGAAT+GGG	+	chr8.3:13249192-13249211	None:intergenic	45.0%
	CCAGAATGGGGCAAATTGAA+GGG	+	chr8.3:13249179-13249198	None:intergenic	45.0%
	CCTTGTCCGTGTCTATAAAC+CGG	-	chr8.3:13250124-13250143	MS.gene61499:CDS	45.0%
	CGTCCAGCCAAAATCAACAA+TGG	-	chr8.3:13249721-13249740	MS.gene61499:CDS	45.0%
	CTCTAATGAGCCATGAAGGA+CGG	+	chr8.3:13250517-13250536	None:intergenic	45.0%
	GGAAACTACTGCTACTACTG+CGG	-	chr8.3:13249430-13249449	MS.gene61499:intron	45.0%
	GTCAATACGAGCTTCTTTGG+AGG	+	chr8.3:13250630-13250649	None:intergenic	45.0%
	TCATGCACCTGTATCCAGAA+TGG	+	chr8.3:13249193-13249212	None:intergenic	45.0%
	TCCAGAATGGGGCAAATTGA+AGG	+	chr8.3:13249180-13249199	None:intergenic	45.0%
	TCCTTTGACAGGATTCCAGA+AGG	+	chr8.3:13249002-13249021	None:intergenic	45.0%
	TCTAGGCTTCATGACAGAGA+AGG	-	chr8.3:13249219-13249238	MS.gene61499:intron	45.0%
	TGCAGAGATGTTCTCATTGC+AGG	+	chr8.3:13249947-13249966	None:intergenic	45.0%
	TGCTACTACTGCGGAATGTT+AGG	-	chr8.3:13249439-13249458	MS.gene61499:intron	45.0%
	TGGAAACGATAGAGACCTTC+TGG	-	chr8.3:13248984-13249003	MS.gene61499:intron	45.0%
	TGGTCGTCGAAGAGAATCAT+GGG	-	chr8.3:13250391-13250410	MS.gene61499:CDS	45.0%
	TTCGTTCTCCTCATCACTGA+GGG	+	chr8.3:13248861-13248880	None:intergenic	45.0%
	TTCTACGGTTACCCAGATTC+TGG	-	chr8.3:13250371-13250390	MS.gene61499:CDS	45.0%
	TTCTCATATAGGAACGGAGG+TGG	+	chr8.3:13249309-13249328	None:intergenic	45.0%
	TTCTCTGTCATGAAGCCTAG+AGG	+	chr8.3:13249220-13249239	None:intergenic	45.0%
	TTGGCTGGACGCATTAATTG+TGG	+	chr8.3:13249712-13249731	None:intergenic	45.0%
	TTGTGTGAAAACGGCCAACA+AGG	+	chr8.3:13248937-13248956	None:intergenic	45.0%
!	AAAGTTTGGAGTGCAGCGTT+TGG	+	chr8.3:13250689-13250708	None:intergenic	45.0%
!	AAGAGGAGTGTGGTCAGATA+TGG	+	chr8.3:13250720-13250739	None:intergenic	45.0%
!	AATACGAGCTTCTTTGGAGG+AGG	+	chr8.3:13250627-13250646	None:intergenic	45.0%
!	ATACGAGCTTCTTTGGAGGA+GGG	+	chr8.3:13250626-13250645	None:intergenic	45.0%
!	CTCTCCTCTGAAGACAAAAG+AGG	-	chr8.3:13250482-13250501	MS.gene61499:CDS	45.0%
!	GGTGATTGTCAAAGTACCAC+GGG	+	chr8.3:13249108-13249127	None:intergenic	45.0%
!	GTTGACCGTATTGGTAGAAG+TGG	-	chr8.3:13250233-13250252	MS.gene61499:CDS	45.0%
!	GTTTGGATAAAGCGTTTGCC+AGG	+	chr8.3:13250672-13250691	None:intergenic	45.0%
!	TCCACCTCTTTTGTCTTCAG+AGG	+	chr8.3:13250489-13250508	None:intergenic	45.0%
!	TCCTCTGAAGACAAAAGAGG+TGG	-	chr8.3:13250485-13250504	MS.gene61499:CDS	45.0%
!	TGACCGTATTGGTAGAAGTG+GGG	-	chr8.3:13250235-13250254	MS.gene61499:CDS	45.0%
!	TGCTTTTCTACACGCAGTTC+AGG	+	chr8.3:13249532-13249551	None:intergenic	45.0%
!!	AAGATCCTGTCATTGGTTCC+TGG	-	chr8.3:13250336-13250355	MS.gene61499:CDS	45.0%
!!	ACCTTCTGGAATCCTGTCAA+AGG	-	chr8.3:13248998-13249017	MS.gene61499:intron	45.0%
!!	GCAGAGTTGGCAGTTTTGAT+TGG	+	chr8.3:13249664-13249683	None:intergenic	45.0%
!!!	ACGCCATTGTTGATTTTGGC+TGG	+	chr8.3:13249727-13249746	None:intergenic	45.0%
!!!	CATTTTTTATCGGCAGAGCC+TGG	-	chr8.3:13250015-13250034	MS.gene61499:CDS	45.0%
	AAGCGTTTGCCAGGAAGAAG+TGG	+	chr8.3:13250663-13250682	None:intergenic	50.0%
	AGGAAGAAGTGGCTAGAGCA+CGG	+	chr8.3:13250652-13250671	None:intergenic	50.0%
	AGGCTTCATGACAGAGAAGG+TGG	-	chr8.3:13249222-13249241	MS.gene61499:intron	50.0%
	ATGATGGCACACGACTATGG+GGG	-	chr8.3:13249506-13249525	MS.gene61499:intron	50.0%
	ATGTGCTAAGGCTGCAGAGT+TGG	+	chr8.3:13249677-13249696	None:intergenic	50.0%
	CAAAGGAAAAACGGAGGAGG+CGG	-	chr8.3:13249550-13249569	MS.gene61499:intron	50.0%
	CAGGTGCATGACATTCCTCT+AGG	-	chr8.3:13249202-13249221	MS.gene61499:intron	50.0%
	CATATAGGAACGGAGGTGGT+TGG	+	chr8.3:13249305-13249324	None:intergenic	50.0%
	CATCACTGAGGGAAAGTTGG+TGG	+	chr8.3:13248850-13248869	None:intergenic	50.0%
	CCAACTTTCCCTCAGTGATG+AGG	-	chr8.3:13248850-13248869	MS.gene61499:intron	50.0%
	CCCCATGGAAGTTTCACACT+CGG	-	chr8.3:13249987-13250006	MS.gene61499:CDS	50.0%
	CCCTTCAATTTGCCCCATTC+TGG	-	chr8.3:13249176-13249195	MS.gene61499:intron	50.0%
	CCGAGTGTGAAACTTCCATG+GGG	+	chr8.3:13249990-13250009	None:intergenic	50.0%
	CCGGTTTATAGACACGGACA+AGG	+	chr8.3:13250127-13250146	None:intergenic	50.0%
	CCTCATCACTGAGGGAAAGT+TGG	+	chr8.3:13248853-13248872	None:intergenic	50.0%
	CGAGTGTGAAACTTCCATGG+GGG	+	chr8.3:13249989-13250008	None:intergenic	50.0%
	CGTCCTTCATGGCTCATTAG+AGG	-	chr8.3:13250515-13250534	MS.gene61499:CDS	50.0%
	CGTTAGAGAGGCTGCTTAGA+TGG	+	chr8.3:13250427-13250446	None:intergenic	50.0%
	CTCCCCCGAATTCTGATTGA+CGG	+	chr8.3:13249582-13249601	None:intergenic	50.0%
	CTCGCCGTCAATCAGAATTC+GGG	-	chr8.3:13249575-13249594	MS.gene61499:intron	50.0%
	CTCTTCGACGACCAGAATCT+GGG	+	chr8.3:13250385-13250404	None:intergenic	50.0%
	CTGGGATCACATTGAGGATC+AGG	+	chr8.3:13249132-13249151	None:intergenic	50.0%
	CTGGTCGTCGAAGAGAATCA+TGG	-	chr8.3:13250390-13250409	MS.gene61499:CDS	50.0%
	GACCCCCACTTCTACCAATA+CGG	+	chr8.3:13250241-13250260	None:intergenic	50.0%
	GAGGATCATCTGTCTGTGTG+AGG	+	chr8.3:13248907-13248926	None:intergenic	50.0%
	GAGGTGGTGTGCAAATTGGA+AGG	+	chr8.3:13249059-13249078	None:intergenic	50.0%
	GCCGAGTGTGAAACTTCCAT+GGG	+	chr8.3:13249991-13250010	None:intergenic	50.0%
	GCTCGCCGTCAATCAGAATT+CGG	-	chr8.3:13249574-13249593	MS.gene61499:intron	50.0%
	GCTTCATGACAGAGAAGGTG+GGG	-	chr8.3:13249224-13249243	MS.gene61499:intron	50.0%
	GGATCACATTGAGGATCAGG+AGG	+	chr8.3:13249129-13249148	None:intergenic	50.0%
	GGCTTCATGACAGAGAAGGT+GGG	-	chr8.3:13249223-13249242	MS.gene61499:intron	50.0%
	GGGCAAATTGAAGGGCAACT+TGG	+	chr8.3:13249171-13249190	None:intergenic	50.0%
	GGTAACCGTAGAAAGCTGTG+AGG	+	chr8.3:13250364-13250383	None:intergenic	50.0%
	TACTCGGCTGATGAGGATGA+TGG	-	chr8.3:13249490-13249509	MS.gene61499:intron	50.0%
	TCGCCGTCAATCAGAATTCG+GGG	-	chr8.3:13249576-13249595	MS.gene61499:intron	50.0%
	TCTCTTCGACGACCAGAATC+TGG	+	chr8.3:13250386-13250405	None:intergenic	50.0%
	TGAATGTAACCATGCCCCCA+TGG	-	chr8.3:13249972-13249991	MS.gene61499:CDS	50.0%
	TGCACCATGGATCGTTAGAG+AGG	+	chr8.3:13250439-13250458	None:intergenic	50.0%
	TGCCGAGTGTGAAACTTCCA+TGG	+	chr8.3:13249992-13250011	None:intergenic	50.0%
!	AGGAGTGTGGTCAGATATGG+AGG	+	chr8.3:13250717-13250736	None:intergenic	50.0%
!	AGGATGATGGCACACGACTA+TGG	-	chr8.3:13249503-13249522	MS.gene61499:intron	50.0%
!	GACCGTATTGGTAGAAGTGG+GGG	-	chr8.3:13250236-13250255	MS.gene61499:CDS	50.0%
!	GATGATGGCACACGACTATG+GGG	-	chr8.3:13249505-13249524	MS.gene61499:intron	50.0%
!	GGATGATGGCACACGACTAT+GGG	-	chr8.3:13249504-13249523	MS.gene61499:intron	50.0%
!	GGATTCTCTGGAAATCGAGG+TGG	+	chr8.3:13249075-13249094	None:intergenic	50.0%
!	TGGGGGCATGGTTACATTCA+TGG	+	chr8.3:13249972-13249991	None:intergenic	50.0%
!!	ATAGGCCTGGCTCTATCTCT+TGG	+	chr8.3:13249033-13249052	None:intergenic	50.0%
!!	GGCCCTTCTTTAGGATTCTC+TGG	+	chr8.3:13249087-13249106	None:intergenic	50.0%
	AGTACCACGGGCCCTTCTTT+AGG	+	chr8.3:13249096-13249115	None:intergenic	55.0%
	ATGAGCCATGAAGGACGGTC+TGG	+	chr8.3:13250512-13250531	None:intergenic	55.0%
	CATGACAGAGAAGGTGGGGA+CGG	-	chr8.3:13249228-13249247	MS.gene61499:intron	55.0%
	CGCCGTCAATCAGAATTCGG+GGG	-	chr8.3:13249577-13249596	MS.gene61499:intron	55.0%
	CGCTGCACTCCAAACTTTGG+TGG	-	chr8.3:13250691-13250710	MS.gene61499:CDS	55.0%
	CGTCAATCAGAATTCGGGGG+AGG	-	chr8.3:13249580-13249599	MS.gene61499:intron	55.0%
	GAAAACGGCCAACAAGGCAG+AGG	+	chr8.3:13248931-13248950	None:intergenic	55.0%
	GAATCCTAAAGAAGGGCCCG+TGG	-	chr8.3:13249089-13249108	MS.gene61499:intron	55.0%
	GCAGCCTCTCTAACGATCCA+TGG	-	chr8.3:13250432-13250451	MS.gene61499:CDS	55.0%
	GCTGTAGGAGAAGAGGAGTG+TGG	+	chr8.3:13250730-13250749	None:intergenic	55.0%
	GTGAAACTTCCATGGGGGCA+TGG	+	chr8.3:13249984-13250003	None:intergenic	55.0%
	GTGCTCTAGCCACTTCTTCC+TGG	-	chr8.3:13250651-13250670	MS.gene61499:CDS	55.0%
	TGGCGCCTCACAGCTTTCTA+CGG	-	chr8.3:13250356-13250375	MS.gene61499:CDS	55.0%
	AGGCGCCAGGAACCAATGAC+AGG	+	chr8.3:13250344-13250363	None:intergenic	60.0%
	ATCTTGGAACTGCCGCGGTC+TGG	-	chr8.3:13250070-13250089	MS.gene61499:CDS	60.0%
	CCTTAACGTTCCCCAGACCG+CGG	+	chr8.3:13250085-13250104	None:intergenic	60.0%
	CGGGGGAGGAGTTTCTTCAC+CGG	-	chr8.3:13249594-13249613	MS.gene61499:intron	60.0%
	GATCCTCGCCTCTGCCTTGT+TGG	-	chr8.3:13248920-13248939	MS.gene61499:intron	60.0%
	GTGGACCAGACCGTCCTTCA+TGG	-	chr8.3:13250504-13250523	MS.gene61499:CDS	60.0%
	GTGGGGGTCTCGCTATCCTT+TGG	-	chr8.3:13250252-13250271	MS.gene61499:CDS	60.0%
	GTGTGAGGGCTTCGTCGATC+TGG	+	chr8.3:13248892-13248911	None:intergenic	60.0%
	TCTTGGAACTGCCGCGGTCT+GGG	-	chr8.3:13250071-13250090	MS.gene61499:CDS	60.0%
	TTCATTGCTTCCGGCAGGCC+TGG	+	chr8.3:13250044-13250063	None:intergenic	60.0%
!	GTAGAAAGCTGTGAGGCGCC+AGG	+	chr8.3:13250357-13250376	None:intergenic	60.0%
	ATCGGCAGAGCCTGGCTTCC+AGG	-	chr8.3:13250023-13250042	MS.gene61499:CDS	65.0%
	CCGCGGTCTGGGGAACGTTA+AGG	-	chr8.3:13250082-13250101	MS.gene61499:CDS	65.0%
	CTTGGAACTGCCGCGGTCTG+GGG	-	chr8.3:13250072-13250091	MS.gene61499:CDS	65.0%
	AGCCTGGCTTCCAGGCCTGC+CGG	-	chr8.3:13250031-13250050	MS.gene61499:CDS	70.0%
	CGGCCAACAAGGCAGAGGCG+AGG	+	chr8.3:13248926-13248945	None:intergenic	70.0%
	TTCCGGCAGGCCTGGAAGCC+AGG	+	chr8.3:13250036-13250055	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	13248836	13250753	13248836	ID=MS.gene61499
chr8.3	mRNA	13248836	13250753	13248836	ID=MS.gene61499.t1;Parent=MS.gene61499
chr8.3	exon	13249613	13250753	13249613	ID=MS.gene61499.t1.exon1;Parent=MS.gene61499.t1
chr8.3	CDS	13249613	13250753	13249613	ID=cds.MS.gene61499.t1;Parent=MS.gene61499.t1
chr8.3	exon	13248836	13248840	13248836	ID=MS.gene61499.t1.exon2;Parent=MS.gene61499.t1
chr8.3	CDS	13248836	13248840	13248836	ID=cds.MS.gene61499.t1;Parent=MS.gene61499.t1

Gene Sequence

>MS.gene61499

ATGACTTCTCTCCACCAACTTTCCCTCAGTGATGAGGAGAACGAATTAGAATTCCAGATCGACGAAGCCCTCACACAGACAGATGATCCTCGCCTCTGCCTTGTTGGCCGTTTTCACACAAACAAGCCAATTCGGTCATATATGATGATGGAAACGATAGAGACCTTCTGGAATCCTGTCAAAGGAGTCAAAATCCAAGAGATAGAGCCAGGCCTATTCACCTTCCAATTTGCACACCACCTCGATTTCCAGAGAATCCTAAAGAAGGGCCCGTGGTACTTTGACAATCACCTCCTGATCCTCAATGTGATCCCAGAGAATATTGCTCCTAACCAAGTTGCCCTTCAATTTGCCCCATTCTGGATACAGGTGCATGACATTCCTCTAGGCTTCATGACAGAGAAGGTGGGGACGGACATTGCGAATTATATTGGTGAATTCCTTGAATATGATGCCAAAAATAATTCCAACCACCTCCGTTCCTATATGAGAATTCGTGTGCTGCTTGATGTTACAAAGCCAGTGAAGCGTCAGAAAAAAATCAAGAGACAAGGTGGGGATTCAAGCTTCATCAAGTTCAAATATGAGCGTCTAGGAAACTACTGCTACTACTGCGGAATGTTAGGCCACATAGAAGATTACTGCGATAAACTTTACTCGGCTGATGAGGATGATGGCACACGACTATGGGGGCCTGAACTGCGTGTAGAAAAGCAAAGGAAAAACGGAGGAGGCGGAGCTCGCCGTCAATCAGAATTCGGGGGAGGAGTTTCTTCACCGGCAGCAGTTACAGTTTCAAACGGTAACGGTAGTGACATTAATGTGCCAATCAAAACTGCCAACTCTGCAGCCTTAGCACATTTACTGCGCAATCCACAATTAATGCGTCCAGCCAAAATCAACAATGGCGTAATCAATAACTCAGTCAATATGGAGCTGTTACAGGAAGAGAATGAAGAGCAAATCATTACCAGCAAACCAAAACGTTCTAGAGGCTTAAATGAGGTCAACCAGTCCTTCAATACAATTAATAACAAATCATCACCTGCAGTTAACGAGGCAGAAATTGATGCATCAGAAACTAGTGCCTTAGCAGACCAAAAATCATCCTGCAATGAGAACATCTCTGCAAGCCATGAATGTAACCATGCCCCCATGGAAGTTTCACACTCGGCAACTCATTTTTTATCGGCAGAGCCTGGCTTCCAGGCCTGCCGGAAGCAATGAATATAATATCTTGGAACTGCCGCGGTCTGGGGAACGTTAAGGCAGTTCCTGAAATTAAAGACCTTGTCCGTGTCTATAAACCGGAAATTGTAATCCTCATTGAAACTTTGGTGAATGGAAATAAAATTTCAGACTTATGCTACTCTATTGGTTTTGATAATCATTTTTCAGTTGACCGTATTGGTAGAAGTGGGGGTCTCGCTATCCTTTGGCGAAATTCTGTTGATTGTTCTCTCATAAACTTCTCCCAAAATTTCATTAACTTGCTAATTAAAGATCCTGTCATTGGTTCCTGGCGCCTCACAGCTTTCTACGGTTACCCAGATTCTGGTCGTCGAAGAGAATCATGGGACTTACTTCACCATCTAAGCAGCCTCTCTAACGATCCATGGTGCATCATTGGTGACTTCAATGACCATCTCTCCTCTGAAGACAAAAGAGGTGGACCAGACCGTCCTTCATGGCTCATTAGAGGATTTCAGGACGCTGTAAAAGACTGCAACTTATATGATATGCCTCTTCATGGTTACCAATTCACATGGTTCAAGAGCATCGGCACGCCCTCCTCCAAAGAAGCTCGTATTGACCGTGCTCTAGCCACTTCTTCCTGGCAAACGCTTTATCCAAACGCTGCACTCCAAACTTTGGTGGCCTCCATATCTGACCACACTCCTCTTCTCCTACAGCTTGA

Protein sequence

>MS.gene61499.t1

MTSLHQLSLSDEENELEFQIDEALTQTDDPRLCLVGRFHTNKPIRSYMMMETIETFWNPVKGVKIQEIEPGLFTFQFAHHLDFQRILKKGPWYFDNHLLILNVIPENIAPNQVALQFAPFWIQVHDIPLGFMTEKVGTDIANYIGEFLEYDAKNNSNHLRSYMRIRVLLDVTKPVKRQKKIKRQGGDSSFIKFKYERLGNYCYYCGMLGHIEDYCDKLYSADEDDGTRLWGPELRVEKQRKNGGGGARRQSEFGGGVSSPAAVTVSNGNGSDINVPIKTANSAALAHLLRNPQLMRPAKINNGVINNSVNMELLQEENEEQIITSKPKRSRGLNEVNQSFNTINNKSSPAVNEAEIDASETSALADQKSSCNENISASHES