AlfalfaGEDB | MS.gene008275

M. sativa cultivar XinJiangDaYe / MS.gene008275

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008275.t1	PNX72859.1	58.9	224	92	0	5	228	124	347	2.20E-76	295.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008275.t1	O04244	27.2	136	95	2	83	216	6	139	6.9e-10	65.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008275.t1	A0A2K3L2W5	58.9	224	92	0	5	228	124	347	1.6e-76	295.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene008275	MS.gene025675	PPI
MS.gene008275	MS.gene94654	PPI
MS.gene008275	MS.gene71637	PPI
MS.gene008275	MS.gene55209	PPI
MS.gene20246	MS.gene008275	PPI
MS.gene008275	MS.gene022929	PPI
MS.gene008275	MS.gene85295	PPI
MS.gene008275	MS.gene52349	PPI
MS.gene008275	MS.gene009781	PPI
MS.gene008275	MS.gene021647	PPI
MS.gene008275	MS.gene047954	PPI
MS.gene008275	MS.gene071784	PPI
MS.gene008275	MS.gene048342	PPI
MS.gene008275	MS.gene069624	PPI
MS.gene008275	MS.gene83979	PPI
MS.gene042559	MS.gene008275	PPI
MS.gene008275	MS.gene013890	PPI
MS.gene008275	MS.gene073582	PPI
MS.gene008275	MS.gene016719	PPI
MS.gene008275	MS.gene048072	PPI
MS.gene008275	MS.gene028714	PPI
MS.gene008275	MS.gene042027	PPI
MS.gene008275	MS.gene048706	PPI
MS.gene69490	MS.gene008275	PPI
MS.gene008275	MS.gene69586	PPI
MS.gene008275	MS.gene45083	PPI
MS.gene015248	MS.gene008275	PPI
MS.gene008275	MS.gene049561	PPI
MS.gene008275	MS.gene008658	PPI
MS.gene008275	MS.gene47792	PPI
MS.gene34812	MS.gene008275	PPI
MS.gene008275	MS.gene49057	PPI
MS.gene008275	MS.gene03936	PPI
MS.gene008275	MS.gene43393	PPI
MS.gene067340	MS.gene008275	PPI
MS.gene008275	MS.gene08708	PPI
MS.gene008275	MS.gene86275	PPI
MS.gene008275	MS.gene034643	PPI
MS.gene008275	MS.gene60059	PPI
MS.gene008275	MS.gene038098	PPI
MS.gene008275	MS.gene071320	PPI
MS.gene05357	MS.gene008275	PPI
MS.gene008275	MS.gene012019	PPI
MS.gene008275	MS.gene46941	PPI
MS.gene008275	MS.gene048747	PPI
MS.gene008275	MS.gene012889	PPI
MS.gene008275	MS.gene038757	PPI
MS.gene008275	MS.gene000506	PPI
MS.gene008275	MS.gene015248	PPI
MS.gene008275	MS.gene030090	PPI
MS.gene008275	MS.gene39956	PPI
MS.gene008275	MS.gene028716	PPI
MS.gene008275	MS.gene74639	PPI
MS.gene008275	MS.gene071133	PPI
MS.gene008275	MS.gene60266	PPI
MS.gene008275	MS.gene98467	PPI
MS.gene008275	MS.gene013644	PPI
MS.gene008275	MS.gene024522	PPI
MS.gene008275	MS.gene54051	PPI
MS.gene008275	MS.gene22111	PPI
MS.gene008275	MS.gene049610	PPI
MS.gene008275	MS.gene010290	PPI
MS.gene008275	MS.gene93396	PPI
MS.gene008275	MS.gene37955	PPI
MS.gene008275	MS.gene95561	PPI
MS.gene008275	MS.gene05358	PPI
MS.gene008275	MS.gene92517	PPI
MS.gene008275	MS.gene34197	PPI
MS.gene008275	MS.gene045875	PPI
MS.gene008275	MS.gene010890	PPI
MS.gene008275	MS.gene84790	PPI
MS.gene008275	MS.gene055683	PPI
MS.gene008275	MS.gene061579	PPI
MS.gene008275	MS.gene05319	PPI
MS.gene008275	MS.gene69495	PPI
MS.gene008275	MS.gene032311	PPI
MS.gene008275	MS.gene41979	PPI
MS.gene008275	MS.gene04970	PPI
MS.gene008275	MS.gene26038	PPI
MS.gene008275	MS.gene060788	PPI
MS.gene008275	MS.gene98616	PPI
MS.gene008275	MS.gene43924	PPI
MS.gene008275	MS.gene23521	PPI
MS.gene008275	MS.gene41643	PPI
MS.gene008275	MS.gene77990	PPI
MS.gene008275	MS.gene65546	PPI
MS.gene008275	MS.gene062511	PPI
MS.gene008275	MS.gene008861	PPI
MS.gene008275	MS.gene065627	PPI
MS.gene008275	MS.gene027994	PPI
MS.gene008275	MS.gene96541	PPI
MS.gene008275	MS.gene26433	PPI
MS.gene008275	MS.gene007022	PPI
MS.gene008275	MS.gene045614	PPI
MS.gene008275	MS.gene064966	PPI
MS.gene008275	MS.gene69584	PPI
MS.gene008275	MS.gene036423	PPI
MS.gene42903	MS.gene008275	PPI
MS.gene008275	MS.gene76420	PPI
MS.gene008275	MS.gene30136	PPI
MS.gene008275	MS.gene46353	PPI
MS.gene008275	MS.gene65471	PPI
MS.gene008275	MS.gene060386	PPI
MS.gene008275	MS.gene61651	PPI
MS.gene22200	MS.gene008275	PPI
MS.gene008275	MS.gene29406	PPI
MS.gene008275	MS.gene63762	PPI
MS.gene008275	MS.gene09023	PPI
MS.gene008275	MS.gene88393	PPI
MS.gene008275	MS.gene054508	PPI
MS.gene94654	MS.gene008275	PPI
MS.gene008275	MS.gene95960	PPI
MS.gene008275	MS.gene053373	PPI
MS.gene008275	MS.gene041264	PPI
MS.gene008275	MS.gene35175	PPI
MS.gene063214	MS.gene008275	PPI
MS.gene008275	MS.gene63150	PPI
MS.gene008275	MS.gene035626	PPI
MS.gene008275	MS.gene44083	PPI
MS.gene008275	MS.gene000629	PPI
MS.gene035626	MS.gene008275	PPI
MS.gene008275	MS.gene072852	PPI
MS.gene008275	MS.gene65474	PPI
MS.gene008275	MS.gene018745	PPI
MS.gene008275	MS.gene025401	PPI
MS.gene008275	MS.gene34812	PPI
MS.gene008275	MS.gene57044	PPI
MS.gene008275	MS.gene015093	PPI
MS.gene008275	MS.gene04134	PPI
MS.gene008275	MS.gene06164	PPI
MS.gene008275	MS.gene50392	PPI
MS.gene008275	MS.gene22730	PPI
MS.gene30208	MS.gene008275	PPI
MS.gene008275	MS.gene000379	PPI
MS.gene008275	MS.gene032299	PPI
MS.gene008275	MS.gene017586	PPI
MS.gene69495	MS.gene008275	PPI
MS.gene008275	MS.gene037634	PPI
MS.gene008275	MS.gene057828	PPI
MS.gene008275	MS.gene08606	PPI
MS.gene008275	MS.gene000640	PPI
MS.gene95561	MS.gene008275	PPI
MS.gene008275	MS.gene061850	PPI
MS.gene008275	MS.gene09517	PPI
MS.gene008275	MS.gene98756	PPI
MS.gene008275	MS.gene87845	PPI
MS.gene008275	MS.gene57019	PPI
MS.gene008275	MS.gene85308	PPI
MS.gene57019	MS.gene008275	PPI
MS.gene008275	MS.gene64130	PPI
MS.gene008275	MS.gene62249	PPI
MS.gene008275	MS.gene58419	PPI
MS.gene008275	MS.gene046089	PPI
MS.gene021968	MS.gene008275	PPI
MS.gene008275	MS.gene053747	PPI
MS.gene008275	MS.gene28545	PPI
MS.gene008275	MS.gene69490	PPI
MS.gene008275	MS.gene021555	PPI
MS.gene008275	MS.gene36069	PPI
MS.gene008275	MS.gene71675	PPI
MS.gene008275	MS.gene044729	PPI
MS.gene008275	MS.gene60390	PPI
MS.gene008275	MS.gene039692	PPI
MS.gene008275	MS.gene55682	PPI
MS.gene008275	MS.gene002590	PPI
MS.gene008275	MS.gene81712	PPI
MS.gene008275	MS.gene008927	PPI
MS.gene008275	MS.gene005120	PPI
MS.gene008275	MS.gene025873	PPI
MS.gene008275	MS.gene05357	PPI
MS.gene008275	MS.gene024297	PPI
MS.gene008275	MS.gene059269	PPI
MS.gene008275	MS.gene35202	PPI
MS.gene008275	MS.gene22200	PPI
MS.gene008275	MS.gene057934	PPI
MS.gene008275	MS.gene37537	PPI
MS.gene008275	MS.gene024841	PPI
MS.gene008275	MS.gene20246	PPI
MS.gene008275	MS.gene069786	PPI
MS.gene008275	MS.gene42010	PPI
MS.gene008275	MS.gene025158	PPI
MS.gene008275	MS.gene40828	PPI
MS.gene008275	MS.gene87529	PPI
MS.gene008275	MS.gene51799	PPI
MS.gene008275	MS.gene98471	PPI
MS.gene008275	MS.gene062067	PPI
MS.gene008275	MS.gene97410	PPI
MS.gene008275	MS.gene56991	PPI
MS.gene96109	MS.gene008275	PPI
MS.gene008275	MS.gene010656	PPI
MS.gene008275	MS.gene013676	PPI
MS.gene008275	MS.gene013193	PPI
MS.gene008275	MS.gene40469	PPI
MS.gene008275	MS.gene65623	PPI
MS.gene008275	MS.gene021968	PPI
MS.gene008275	MS.gene012748	PPI
MS.gene008275	MS.gene063214	PPI
MS.gene008275	MS.gene66651	PPI
MS.gene008275	MS.gene025987	PPI
MS.gene008275	MS.gene043140	PPI
MS.gene008275	MS.gene02896	PPI
MS.gene008275	MS.gene71588	PPI
MS.gene008275	MS.gene72520	PPI
MS.gene008275	MS.gene61452	PPI
MS.gene008275	MS.gene05231	PPI
MS.gene008275	MS.gene03149	PPI
MS.gene008275	MS.gene042559	PPI
MS.gene008275	MS.gene46948	PPI
MS.gene008275	MS.gene29321	PPI
MS.gene016102	MS.gene008275	PPI
MS.gene46941	MS.gene008275	PPI
MS.gene96541	MS.gene008275	PPI
MS.gene008275	MS.gene037049	PPI
MS.gene008275	MS.gene24278	PPI
MS.gene008275	MS.gene34618	PPI
MS.gene025873	MS.gene008275	PPI
MS.gene008275	MS.gene59460	PPI
MS.gene008275	MS.gene060773	PPI
MS.gene008275	MS.gene66650	PPI
MS.gene008275	MS.gene009121	PPI
MS.gene05358	MS.gene008275	PPI
MS.gene008275	MS.gene08713	PPI
MS.gene008275	MS.gene09589	PPI
MS.gene008275	MS.gene70932	PPI
MS.gene008275	MS.gene024405	PPI
MS.gene065627	MS.gene008275	PPI
MS.gene008275	MS.gene067340	PPI
MS.gene008275	MS.gene021965	PPI
MS.gene008275	MS.gene018196	PPI
MS.gene008275	MS.gene32415	PPI
MS.gene008275	MS.gene24070	PPI
MS.gene008275	MS.gene83772	PPI
MS.gene008275	MS.gene73261	PPI
MS.gene008275	MS.gene059736	PPI
MS.gene008275	MS.gene85538	PPI
MS.gene008275	MS.gene26844	PPI
MS.gene008275	MS.gene64178	PPI
MS.gene008275	MS.gene055546	PPI
MS.gene008275	MS.gene96174	PPI
MS.gene008275	MS.gene074257	PPI
MS.gene56991	MS.gene008275	PPI
MS.gene008275	MS.gene04749	PPI
MS.gene008275	MS.gene073573	PPI
MS.gene008275	MS.gene28718	PPI
MS.gene008275	MS.gene44144	PPI
MS.gene008275	MS.gene05576	PPI
MS.gene28545	MS.gene008275	PPI
MS.gene008275	MS.gene060718	PPI
MS.gene008275	MS.gene96109	PPI
MS.gene008275	MS.gene63743	PPI
MS.gene008275	MS.gene016102	PPI
MS.gene008275	MS.gene48477	PPI
MS.gene008275	MS.gene055223	PPI
MS.gene008275	MS.gene068629	PPI
MS.gene008275	MS.gene08106	PPI
MS.gene008275	MS.gene027169	PPI
MS.gene008275	MS.gene004417	PPI
MS.gene008275	MS.gene019177	PPI
MS.gene008275	MS.gene031967	PPI
MS.gene008275	MS.gene05436	PPI
MS.gene008275	MS.gene038623	PPI
MS.gene008275	MS.gene21066	PPI
MS.gene008275	MS.gene055560	PPI
MS.gene008275	MS.gene009213	PPI
MS.gene008275	MS.gene98127	PPI
MS.gene008275	MS.gene003264	PPI
MS.gene008275	MS.gene054652	PPI
MS.gene008275	MS.gene000634	PPI
MS.gene008275	MS.gene86849	PPI
MS.gene008275	MS.gene049512	PPI
MS.gene008275	MS.gene27329	PPI
MS.gene008275	MS.gene41393	PPI
MS.gene008275	MS.gene42903	PPI
MS.gene008275	MS.gene30208	PPI
MS.gene008275	MS.gene80362	PPI
MS.gene72520	MS.gene008275	PPI
MS.gene013193	MS.gene008275	PPI
MS.gene008275	MS.gene89815	PPI
MS.gene08106	MS.gene008275	PPI
MS.gene008275	MS.gene016632	PPI
MS.gene008275	MS.gene018474	PPI
MS.gene008275	MS.gene048088	PPI
MS.gene008275	MS.gene86868	PPI
MS.gene008275	MS.gene058889	PPI
MS.gene008275	MS.gene96163	PPI
MS.gene008275	MS.gene050679	PPI
MS.gene008275	MS.gene34463	PPI
MS.gene93396	MS.gene008275	PPI
MS.gene008275	MS.gene042614	PPI
MS.gene008275	MS.gene026680	PPI
MS.gene008275	MS.gene61499	PPI
MS.gene008275	MS.gene51804	PPI
MS.gene008275	MS.gene051449	PPI
MS.gene008275	MS.gene042651	PPI
MS.gene038676	MS.gene008275	PPI
MS.gene008275	MS.gene019165	PPI
MS.gene008275	MS.gene81194	PPI
MS.gene008275	MS.gene038394	PPI
MS.gene008275	MS.gene019001	PPI
MS.gene008275	MS.gene038676	PPI
MS.gene008275	MS.gene53904	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008275.t1	MTR_3g053920	67.052	173	42	1	1	173	1	158	7.98e-82	246
MS.gene008275.t1	MTR_2g061500	73.643	129	34	0	1	129	1	129	2.33e-68	206
MS.gene008275.t1	MTR_4g103530	72.165	97	27	0	55	151	1	97	1.03e-48	158
MS.gene008275.t1	MTR_7g011710	71.765	85	24	0	55	139	1	85	5.38e-41	137
MS.gene008275.t1	MTR_6g033215	34.010	197	123	3	5	199	103	294	1.24e-38	135
MS.gene008275.t1	MTR_0010s0370	32.955	176	112	4	7	177	3	177	3.80e-29	108
MS.gene008275.t1	MTR_0010s0350	32.955	176	112	4	7	177	3	177	3.80e-29	108
MS.gene008275.t1	MTR_1g036720	32.323	99	65	1	11	107	7	105	8.28e-13	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008275.t1	AT2G17920	26.977	215	147	5	8	216	9	219	1.62e-17	80.1
MS.gene008275.t1	AT3G31430	26.761	213	146	4	8	215	104	311	1.82e-16	77.4
MS.gene008275.t1	AT5G36228	26.396	197	138	3	36	227	39	233	2.03e-16	77.4
MS.gene008275.t1	AT2G41590	25.806	186	130	3	35	216	38	219	6.17e-15	73.2
MS.gene008275.t1	AT3G57587	25.676	148	106	2	71	216	74	219	1.23e-14	72.4
MS.gene008275.t1	AT2G02103	26.486	185	128	3	36	216	39	219	3.23e-14	71.2
MS.gene008275.t1	AT2G13450	26.486	185	128	4	35	215	38	218	3.57e-14	71.2
MS.gene008275.t1	AT5G25200	26.203	187	126	4	36	216	39	219	3.64e-14	71.2
MS.gene008275.t1	AT5G18636	26.203	187	126	4	36	216	39	219	4.12e-14	70.9
MS.gene008275.t1	AT3G47920	26.351	148	105	2	71	216	3	148	9.22e-13	66.6
MS.gene008275.t1	AT4G02000	27.206	136	95	2	83	216	6	139	1.47e-11	63.2

Find 73 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGCTTGATCGGTCGTGTTT+TGG	0.246456	3.1:-54923482	MS.gene008275:CDS
CATTGAATCACCTGGACATT+TGG	0.249024	3.1:-54923237	MS.gene008275:CDS
CATCAAGTTTGTGGTGAAAC+TGG	0.304253	3.1:+54923353	None:intergenic
TGTTACTGTACCACTGAAAA+AGG	0.308378	3.1:-54923074	MS.gene008275:CDS
CAGGTCCACGGTTTGCCCTT+TGG	0.316867	3.1:-54923211	MS.gene008275:CDS
GCTTGCCTGGTTTCCATGCT+CGG	0.336051	3.1:+54923416	None:intergenic
CAATGTCGGCAGCTAGGTTA+AGG	0.345236	3.1:+54923527	None:intergenic
CAAGGAATTGGCAGTTTCCT+TGG	0.351997	3.1:-54923166	MS.gene008275:CDS
ATTGAATCACCTGGACATTT+GGG	0.353010	3.1:-54923236	MS.gene008275:CDS
CCAATTCCTTGACCAACCTT+TGG	0.354457	3.1:+54923178	None:intergenic
CCAAAGGTTGGTCAAGGAAT+TGG	0.363573	3.1:-54923178	MS.gene008275:CDS
TTCTCATATTTAAACAAAAC+AGG	0.373656	3.1:+54923016	None:intergenic
TGTTACAGGTGTGGCATTCT+CGG	0.390750	3.1:-54922980	MS.gene008275:CDS
TGAAAACTTTCATATAGTTA+TGG	0.408965	3.1:-54923296	MS.gene008275:CDS
CCTCACTTTGAGTCGCATAA+AGG	0.417235	3.1:+54923102	None:intergenic
CCCACGAACCTTAACATTGA+TGG	0.418920	3.1:-54923577	MS.gene008275:CDS
GTGTCCGGAGCTGTTCGAAC+TGG	0.431938	3.1:-54922942	MS.gene008275:CDS
GACCGATCAAGCAGTGTTCA+AGG	0.432003	3.1:+54923491	None:intergenic
AGGAACTGTGAATGGTATTA+CGG	0.436819	3.1:+54923122	None:intergenic
GTGTGGCATTCTCGGTCATT+CGG	0.451796	3.1:-54922972	MS.gene008275:CDS
CTTGCCTGGTTTCCATGCTC+GGG	0.461634	3.1:+54923417	None:intergenic
CACGAGCAACATCAAGTTTG+TGG	0.464404	3.1:+54923344	None:intergenic
ACCATCAATGTTAAGGTTCG+TGG	0.472453	3.1:+54923576	None:intergenic
ATCCTTGAACACTGCTTGAT+CGG	0.474216	3.1:-54923493	MS.gene008275:CDS
TGATGATGGTGTGCGCAACT+AGG	0.474537	3.1:-54922915	None:intergenic
GGGTAAGACCATCAATGTTA+AGG	0.477421	3.1:+54923569	None:intergenic
GACATTTGGGTGCAGGTCCA+CGG	0.479487	3.1:-54923223	MS.gene008275:CDS
ATGATTGTCACACGCTTGCC+TGG	0.479590	3.1:+54923403	None:intergenic
GGGTCAAACCCTCATCAGCC+AGG	0.480642	3.1:+54923548	None:intergenic
CAAATGTCCAGGTGATTCAA+TGG	0.494410	3.1:+54923238	None:intergenic
TCATTCGGACAAGGTGTGTC+CGG	0.496311	3.1:-54922957	MS.gene008275:CDS
AATGTCGGCAGCTAGGTTAA+GGG	0.497192	3.1:+54923528	None:intergenic
CTTTGGATTCATTCAACCAA+AGG	0.499724	3.1:-54923194	MS.gene008275:CDS
TGGTCTTACCCTGGCTGATG+AGG	0.501493	3.1:-54923557	MS.gene008275:CDS
CACTGTTCCATTGAATCACC+TGG	0.504618	3.1:-54923245	MS.gene008275:CDS
GTTAAGGTTCGTGGGATCCA+TGG	0.509655	3.1:+54923585	None:intergenic
AGCGGCTTTGATTTCTCGAT+CGG	0.509809	3.1:+54923459	None:intergenic
TCACCTGGACATTTGGGTGC+AGG	0.517621	3.1:-54923230	MS.gene008275:CDS
TCGCATAAAGGAACTGTGAA+TGG	0.520321	3.1:+54923114	None:intergenic
GTGTGTTGGACGAGGGTCCA+TGG	0.521077	3.1:-54923324	MS.gene008275:CDS
TGTTGCTCGTGTGTTGGACG+AGG	0.522450	3.1:-54923332	MS.gene008275:CDS
GGATTCATTCAACCAAAGGT+TGG	0.527032	3.1:-54923190	MS.gene008275:CDS
GATGATGGTGTGCGCAACTA+GGG	0.529487	3.1:-54922914	None:intergenic
GGTCTTACCCTGGCTGATGA+GGG	0.530738	3.1:-54923556	MS.gene008275:CDS
GGACCTGCACCCAAATGTCC+AGG	0.535494	3.1:+54923227	None:intergenic
GAGTCCAGTTCGAACAGCTC+CGG	0.535698	3.1:+54922938	None:intergenic
ATGTCCCGAGCATGGAAACC+AGG	0.539941	3.1:-54923421	MS.gene008275:CDS
TTCGAACTGGACTCTGATGA+TGG	0.548007	3.1:-54922929	MS.gene008275:CDS
GATCAAGCAGTGTTCAAGGA+TGG	0.553362	3.1:+54923495	None:intergenic
ATTCAACCAAAGGTTGGTCA+AGG	0.554054	3.1:-54923184	MS.gene008275:CDS
GTTATGGAACGTATTGCTCC+TGG	0.556965	3.1:-54923280	MS.gene008275:CDS
TTTGGTTGAATGAATCCAAA+GGG	0.557289	3.1:+54923196	None:intergenic
ATCAAGCAGTGTTCAAGGAT+GGG	0.561405	3.1:+54923496	None:intergenic
CATTCTCGGTCATTCGGACA+AGG	0.565279	3.1:-54922966	MS.gene008275:CDS
ACTTGATGTTGCTCGTGTGT+TGG	0.565320	3.1:-54923338	MS.gene008275:CDS
AGGACTCAATGTCGGCAGCT+AGG	0.567310	3.1:+54923521	None:intergenic
TTGTTTAAATATGAGAAGCT+TGG	0.568801	3.1:-54923010	MS.gene008275:CDS
CCATCAATGTTAAGGTTCGT+GGG	0.580051	3.1:+54923577	None:intergenic
ACAGTGGTCGAGACAACACC+AGG	0.584103	3.1:+54923262	None:intergenic
CTTTGGTTGAATGAATCCAA+AGG	0.593606	3.1:+54923195	None:intergenic
CGTAAGAACCATTGTTAGCA+CGG	0.593728	3.1:+54923041	None:intergenic
GTGAACGCATGTCCCGAGCA+TGG	0.594541	3.1:-54923429	MS.gene008275:CDS
CCTTTATGCGACTCAAAGTG+AGG	0.598255	3.1:-54923102	MS.gene008275:CDS
GGTCAAACCCTCATCAGCCA+GGG	0.622364	3.1:+54923549	None:intergenic
TAACATTGATGGTCTTACCC+TGG	0.623982	3.1:-54923566	MS.gene008275:CDS
TGGAAAGTCCGTGCTAACAA+TGG	0.624040	3.1:-54923049	MS.gene008275:CDS
GATGGGAGAGGACTCAATGT+CGG	0.638132	3.1:+54923513	None:intergenic
GTTGCTCGTGTGTTGGACGA+GGG	0.650160	3.1:-54923331	MS.gene008275:CDS
CATGCGTTCACTGAAGTAAG+CGG	0.663600	3.1:+54923441	None:intergenic
GCAGTGTTCAAGGATGGGAG+AGG	0.664768	3.1:+54923501	None:intergenic
TGAATCCAAAGGGCAAACCG+TGG	0.676765	3.1:+54923206	None:intergenic
CAGGTGATTCAATGGAACAG+TGG	0.687191	3.1:+54923246	None:intergenic
CATATGTGTGTAAAGCACCA+AGG	0.691205	3.1:+54923149	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAAAACTTTCATATAGTTA+TGG	-	chr3.1:54923203-54923222	MS.gene008275:CDS	20.0%
!!	TTCTCATATTTAAACAAAAC+AGG	+	chr3.1:54923486-54923505	None:intergenic	20.0%
!	TTGTTTAAATATGAGAAGCT+TGG	-	chr3.1:54923489-54923508	MS.gene008275:CDS	25.0%
	TTTGGTTGAATGAATCCAAA+GGG	+	chr3.1:54923306-54923325	None:intergenic	30.0%
!	TGAAAGTTTTCATATAGCCA+TGG	+	chr3.1:54923195-54923214	None:intergenic	30.0%
	AGGAACTGTGAATGGTATTA+CGG	+	chr3.1:54923380-54923399	None:intergenic	35.0%
	ATTGAATCACCTGGACATTT+GGG	-	chr3.1:54923263-54923282	MS.gene008275:CDS	35.0%
	CTTTGGATTCATTCAACCAA+AGG	-	chr3.1:54923305-54923324	MS.gene008275:CDS	35.0%
	CTTTGGTTGAATGAATCCAA+AGG	+	chr3.1:54923307-54923326	None:intergenic	35.0%
	TGTTACTGTACCACTGAAAA+AGG	-	chr3.1:54923425-54923444	MS.gene008275:CDS	35.0%
!!!	AGCTTGGTGTTTTTTGTTAC+AGG	-	chr3.1:54923505-54923524	MS.gene008275:CDS	35.0%
	ACCATCAATGTTAAGGTTCG+TGG	+	chr3.1:54922926-54922945	None:intergenic	40.0%
	ATCAAGCAGTGTTCAAGGAT+GGG	+	chr3.1:54923006-54923025	None:intergenic	40.0%
	ATCCTTGAACACTGCTTGAT+CGG	-	chr3.1:54923006-54923025	MS.gene008275:CDS	40.0%
	CAAATGTCCAGGTGATTCAA+TGG	+	chr3.1:54923264-54923283	None:intergenic	40.0%
	CATCAAGTTTGTGGTGAAAC+TGG	+	chr3.1:54923149-54923168	None:intergenic	40.0%
	CATTGAATCACCTGGACATT+TGG	-	chr3.1:54923262-54923281	MS.gene008275:CDS	40.0%
	CCATCAATGTTAAGGTTCGT+GGG	+	chr3.1:54922925-54922944	None:intergenic	40.0%
	CGTAAGAACCATTGTTAGCA+CGG	+	chr3.1:54923461-54923480	None:intergenic	40.0%
	GGATTCATTCAACCAAAGGT+TGG	-	chr3.1:54923309-54923328	MS.gene008275:CDS	40.0%
	GGGTAAGACCATCAATGTTA+AGG	+	chr3.1:54922933-54922952	None:intergenic	40.0%
	TCGCATAAAGGAACTGTGAA+TGG	+	chr3.1:54923388-54923407	None:intergenic	40.0%
!	ATTCAACCAAAGGTTGGTCA+AGG	-	chr3.1:54923315-54923334	MS.gene008275:CDS	40.0%
!	CATATGTGTGTAAAGCACCA+AGG	+	chr3.1:54923353-54923372	None:intergenic	40.0%
!	TAACATTGATGGTCTTACCC+TGG	-	chr3.1:54922933-54922952	MS.gene008275:CDS	40.0%
!!!	ACTTTCCACTCCTTTTTCAG+TGG	+	chr3.1:54923438-54923457	None:intergenic	40.0%
!!!	GGTGTTTTTTGTTACAGGTG+TGG	-	chr3.1:54923510-54923529	MS.gene008275:CDS	40.0%
	AATGTCGGCAGCTAGGTTAA+GGG	+	chr3.1:54922974-54922993	None:intergenic	45.0%
	ACTTGATGTTGCTCGTGTGT+TGG	-	chr3.1:54923161-54923180	MS.gene008275:CDS	45.0%
	CAAGGAATTGGCAGTTTCCT+TGG	-	chr3.1:54923333-54923352	MS.gene008275:CDS	45.0%
	CACGAGCAACATCAAGTTTG+TGG	+	chr3.1:54923158-54923177	None:intergenic	45.0%
	CACTGTTCCATTGAATCACC+TGG	-	chr3.1:54923254-54923273	MS.gene008275:CDS	45.0%
	CAGGTGATTCAATGGAACAG+TGG	+	chr3.1:54923256-54923275	None:intergenic	45.0%
	CATGCGTTCACTGAAGTAAG+CGG	+	chr3.1:54923061-54923080	None:intergenic	45.0%
	CCAATTCCTTGACCAACCTT+TGG	+	chr3.1:54923324-54923343	None:intergenic	45.0%
	CCCACGAACCTTAACATTGA+TGG	-	chr3.1:54922922-54922941	MS.gene008275:CDS	45.0%
	CCTCACTTTGAGTCGCATAA+AGG	+	chr3.1:54923400-54923419	None:intergenic	45.0%
	CCTTTATGCGACTCAAAGTG+AGG	-	chr3.1:54923397-54923416	MS.gene008275:CDS	45.0%
	CTGTACCACTGAAAAAGGAG+TGG	-	chr3.1:54923430-54923449	MS.gene008275:CDS	45.0%
	GATCAAGCAGTGTTCAAGGA+TGG	+	chr3.1:54923007-54923026	None:intergenic	45.0%
	GTTATGGAACGTATTGCTCC+TGG	-	chr3.1:54923219-54923238	MS.gene008275:CDS	45.0%
	TGGAAAGTCCGTGCTAACAA+TGG	-	chr3.1:54923450-54923469	MS.gene008275:CDS	45.0%
	TGTTACAGGTGTGGCATTCT+CGG	-	chr3.1:54923519-54923538	MS.gene008275:CDS	45.0%
	TTCGAACTGGACTCTGATGA+TGG	-	chr3.1:54923570-54923589	MS.gene008275:CDS	45.0%
!	AGCGGCTTTGATTTCTCGAT+CGG	+	chr3.1:54923043-54923062	None:intergenic	45.0%
!	CCAAAGGTTGGTCAAGGAAT+TGG	-	chr3.1:54923321-54923340	MS.gene008275:CDS	45.0%
	ATGATTGTCACACGCTTGCC+TGG	+	chr3.1:54923099-54923118	None:intergenic	50.0%
	CAATGTCGGCAGCTAGGTTA+AGG	+	chr3.1:54922975-54922994	None:intergenic	50.0%
	CATTCTCGGTCATTCGGACA+AGG	-	chr3.1:54923533-54923552	MS.gene008275:CDS	50.0%
	GACCGATCAAGCAGTGTTCA+AGG	+	chr3.1:54923011-54923030	None:intergenic	50.0%
	GATGGGAGAGGACTCAATGT+CGG	+	chr3.1:54922989-54923008	None:intergenic	50.0%
	GTGTGGCATTCTCGGTCATT+CGG	-	chr3.1:54923527-54923546	MS.gene008275:CDS	50.0%
	TCATTCGGACAAGGTGTGTC+CGG	-	chr3.1:54923542-54923561	MS.gene008275:CDS	50.0%
	TGAATCCAAAGGGCAAACCG+TGG	+	chr3.1:54923296-54923315	None:intergenic	50.0%
!	CTGCTTGATCGGTCGTGTTT+TGG	-	chr3.1:54923017-54923036	MS.gene008275:CDS	50.0%
	ACAGTGGTCGAGACAACACC+AGG	+	chr3.1:54923240-54923259	None:intergenic	55.0%
	AGGACTCAATGTCGGCAGCT+AGG	+	chr3.1:54922981-54923000	None:intergenic	55.0%
	ATGTCCCGAGCATGGAAACC+AGG	-	chr3.1:54923078-54923097	MS.gene008275:CDS	55.0%
	CTTGCCTGGTTTCCATGCTC+GGG	+	chr3.1:54923085-54923104	None:intergenic	55.0%
	GACATTTGGGTGCAGGTCCA+CGG	-	chr3.1:54923276-54923295	MS.gene008275:CDS	55.0%
	GAGTCCAGTTCGAACAGCTC+CGG	+	chr3.1:54923564-54923583	None:intergenic	55.0%
	GCAGTGTTCAAGGATGGGAG+AGG	+	chr3.1:54923001-54923020	None:intergenic	55.0%
	GCTTGCCTGGTTTCCATGCT+CGG	+	chr3.1:54923086-54923105	None:intergenic	55.0%
	GGTCAAACCCTCATCAGCCA+GGG	+	chr3.1:54922953-54922972	None:intergenic	55.0%
	GGTCTTACCCTGGCTGATGA+GGG	-	chr3.1:54922943-54922962	MS.gene008275:CDS	55.0%
	GTTGCTCGTGTGTTGGACGA+GGG	-	chr3.1:54923168-54923187	MS.gene008275:CDS	55.0%
	TCACCTGGACATTTGGGTGC+AGG	-	chr3.1:54923269-54923288	MS.gene008275:CDS	55.0%
	TGTTGCTCGTGTGTTGGACG+AGG	-	chr3.1:54923167-54923186	MS.gene008275:CDS	55.0%
!	TGGTCTTACCCTGGCTGATG+AGG	-	chr3.1:54922942-54922961	MS.gene008275:CDS	55.0%
	CAGGTCCACGGTTTGCCCTT+TGG	-	chr3.1:54923288-54923307	MS.gene008275:CDS	60.0%
	GGACCTGCACCCAAATGTCC+AGG	+	chr3.1:54923275-54923294	None:intergenic	60.0%
	GGGTCAAACCCTCATCAGCC+AGG	+	chr3.1:54922954-54922973	None:intergenic	60.0%
	GTGAACGCATGTCCCGAGCA+TGG	-	chr3.1:54923070-54923089	MS.gene008275:CDS	60.0%
	GTGTCCGGAGCTGTTCGAAC+TGG	-	chr3.1:54923557-54923576	MS.gene008275:CDS	60.0%
	GTGTGTTGGACGAGGGTCCA+TGG	-	chr3.1:54923175-54923194	MS.gene008275:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	54922916	54923605	54922916	ID=MS.gene008275
chr3.1	mRNA	54922916	54923605	54922916	ID=MS.gene008275.t1;Parent=MS.gene008275
chr3.1	exon	54922916	54923605	54922916	ID=MS.gene008275.t1.exon1;Parent=MS.gene008275.t1
chr3.1	CDS	54922916	54923605	54922916	ID=cds.MS.gene008275.t1;Parent=MS.gene008275.t1

Gene Sequence

>MS.gene008275

ATGGATCCCACGAACCTTAACATTGATGGTCTTACCCTGGCTGATGAGGGTTTGACCCTTAACCTAGCTGCCGACATTGAGTCCTCTCCCATCCTTGAACACTGCTTGATCGGTCGTGTTTTGGCCGATCGAGAAATCAAAGCCGCTTACTTCAGTGAACGCATGTCCCGAGCATGGAAACCAGGCAAGCGTGTGACAATCATCAAATCTGATGCAGATCGATTTCTGTTCCAGTTTCACCACAAACTTGATGTTGCTCGTGTGTTGGACGAGGGTCCATGGCTATATGAAAACTTTCATATAGTTATGGAACGTATTGCTCCTGGTGTTGTCTCGACCACTGTTCCATTGAATCACCTGGACATTTGGGTGCAGGTCCACGGTTTGCCCTTTGGATTCATTCAACCAAAGGTTGGTCAAGGAATTGGCAGTTTCCTTGGTGCTTTACACACATATGATAGCCGTAATACCATTCACAGTTCCTTTATGCGACTCAAAGTGAGGATTGATGTTACTGTACCACTGAAAAAGGAGTGGAAAGTCCGTGCTAACAATGGTTCTTACGTTCCTGTTTTGTTTAAATATGAGAAGCTTGGTGTTTTTTGTTACAGGTGTGGCATTCTCGGTCATTCGGACAAGGTGTGTCCGGAGCTGTTCGAACTGGACTCTGATGATGGTGTGCGCAACTAG

Protein sequence

>MS.gene008275.t1

MDPTNLNIDGLTLADEGLTLNLAADIESSPILEHCLIGRVLADREIKAAYFSERMSRAWKPGKRVTIIKSDADRFLFQFHHKLDVARVLDEGPWLYENFHIVMERIAPGVVSTTVPLNHLDIWVQVHGLPFGFIQPKVGQGIGSFLGALHTYDSRNTIHSSFMRLKVRIDVTVPLKKEWKVRANNGSYVPVLFKYEKLGVFCYRCGILGHSDKVCPELFELDSDDGVRN