AlfalfaGEDB | MS.gene87845

M. sativa cultivar XinJiangDaYe / MS.gene87845

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87845.t1	AES70414.1	59.9	347	71	4	1	346	1	280	5.20E-104	388.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87845.t1	O04244	27.9	136	94	2	83	216	6	139	4.4e-11	70.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87845.t1	G7J017	59.9	347	71	4	1	346	1	280	3.8e-104	388.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061850	MS.gene87845	PPI
MS.gene87845	MS.gene048088	PPI
MS.gene87845	MS.gene86868	PPI
MS.gene26433	MS.gene87845	PPI
MS.gene22730	MS.gene87845	PPI
MS.gene073582	MS.gene87845	PPI
MS.gene042559	MS.gene87845	PPI
MS.gene058889	MS.gene87845	PPI
MS.gene26038	MS.gene87845	PPI
MS.gene042027	MS.gene87845	PPI
MS.gene85308	MS.gene87845	PPI
MS.gene69490	MS.gene87845	PPI
MS.gene87845	MS.gene012889	PPI
MS.gene87845	MS.gene000506	PPI
MS.gene015248	MS.gene87845	PPI
MS.gene34812	MS.gene87845	PPI
MS.gene44083	MS.gene87845	PPI
MS.gene55682	MS.gene87845	PPI
MS.gene04970	MS.gene87845	PPI
MS.gene87845	MS.gene028716	PPI
MS.gene08106	MS.gene87845	PPI
MS.gene87845	MS.gene74639	PPI
MS.gene51799	MS.gene87845	PPI
MS.gene58419	MS.gene87845	PPI
MS.gene87845	MS.gene54051	PPI
MS.gene067340	MS.gene87845	PPI
MS.gene23521	MS.gene87845	PPI
MS.gene019177	MS.gene87845	PPI
MS.gene049512	MS.gene87845	PPI
MS.gene87845	MS.gene010890	PPI
MS.gene29406	MS.gene87845	PPI
MS.gene038623	MS.gene87845	PPI
MS.gene86275	MS.gene87845	PPI
MS.gene05357	MS.gene87845	PPI
MS.gene87529	MS.gene87845	PPI
MS.gene004417	MS.gene87845	PPI
MS.gene04134	MS.gene87845	PPI
MS.gene062067	MS.gene87845	PPI
MS.gene50392	MS.gene87845	PPI
MS.gene072852	MS.gene87845	PPI
MS.gene87845	MS.gene060788	PPI
MS.gene57044	MS.gene87845	PPI
MS.gene89815	MS.gene87845	PPI
MS.gene39956	MS.gene87845	PPI
MS.gene017586	MS.gene87845	PPI
MS.gene87845	MS.gene027994	PPI
MS.gene87845	MS.gene007022	PPI
MS.gene71637	MS.gene87845	PPI
MS.gene09589	MS.gene87845	PPI
MS.gene87845	MS.gene69584	PPI
MS.gene87845	MS.gene52349	PPI
MS.gene057828	MS.gene87845	PPI
MS.gene87845	MS.gene46353	PPI
MS.gene66650	MS.gene87845	PPI
MS.gene87845	MS.gene61651	PPI
MS.gene005120	MS.gene87845	PPI
MS.gene060386	MS.gene87845	PPI
MS.gene71588	MS.gene87845	PPI
MS.gene87845	MS.gene016719	PPI
MS.gene04749	MS.gene87845	PPI
MS.gene87845	MS.gene88393	PPI
MS.gene87845	MS.gene054508	PPI
MS.gene87845	MS.gene053373	PPI
MS.gene059736	MS.gene87845	PPI
MS.gene027169	MS.gene87845	PPI
MS.gene87845	MS.gene63150	PPI
MS.gene42903	MS.gene87845	PPI
MS.gene81712	MS.gene87845	PPI
MS.gene026680	MS.gene87845	PPI
MS.gene87845	MS.gene65474	PPI
MS.gene021555	MS.gene87845	PPI
MS.gene87845	MS.gene025401	PPI
MS.gene87845	MS.gene08708	PPI
MS.gene048747	MS.gene87845	PPI
MS.gene87845	MS.gene06164	PPI
MS.gene22200	MS.gene87845	PPI
MS.gene87845	MS.gene50392	PPI
MS.gene87845	MS.gene22730	PPI
MS.gene019165	MS.gene87845	PPI
MS.gene87845	MS.gene032299	PPI
MS.gene87845	MS.gene08606	PPI
MS.gene94654	MS.gene87845	PPI
MS.gene87845	MS.gene000640	PPI
MS.gene073573	MS.gene87845	PPI
MS.gene031967	MS.gene87845	PPI
MS.gene87845	MS.gene98756	PPI
MS.gene02896	MS.gene87845	PPI
MS.gene063214	MS.gene87845	PPI
MS.gene97410	MS.gene87845	PPI
MS.gene83772	MS.gene87845	PPI
MS.gene32415	MS.gene87845	PPI
MS.gene87845	MS.gene071133	PPI
MS.gene87845	MS.gene60266	PPI
MS.gene009213	MS.gene87845	PPI
MS.gene87845	MS.gene62249	PPI
MS.gene035626	MS.gene87845	PPI
MS.gene87845	MS.gene22111	PPI
MS.gene87845	MS.gene010290	PPI
MS.gene63762	MS.gene87845	PPI
MS.gene87845	MS.gene37955	PPI
MS.gene65623	MS.gene87845	PPI
MS.gene71675	MS.gene87845	PPI
MS.gene87845	MS.gene92517	PPI
MS.gene30208	MS.gene87845	PPI
MS.gene028716	MS.gene87845	PPI
MS.gene69495	MS.gene87845	PPI
MS.gene87845	MS.gene84790	PPI
MS.gene87845	MS.gene044729	PPI
MS.gene55209	MS.gene87845	PPI
MS.gene87845	MS.gene60390	PPI
MS.gene032311	MS.gene87845	PPI
MS.gene87845	MS.gene039692	PPI
MS.gene64178	MS.gene87845	PPI
MS.gene030090	MS.gene87845	PPI
MS.gene96174	MS.gene87845	PPI
MS.gene018196	MS.gene87845	PPI
MS.gene86849	MS.gene87845	PPI
MS.gene57019	MS.gene87845	PPI
MS.gene87845	MS.gene77990	PPI
MS.gene87845	MS.gene062511	PPI
MS.gene03936	MS.gene87845	PPI
MS.gene021968	MS.gene87845	PPI
MS.gene87845	MS.gene059269	PPI
MS.gene87845	MS.gene057934	PPI
MS.gene87845	MS.gene024841	PPI
MS.gene60059	MS.gene87845	PPI
MS.gene43393	MS.gene87845	PPI
MS.gene87845	MS.gene025158	PPI
MS.gene34197	MS.gene87845	PPI
MS.gene008275	MS.gene87845	PPI
MS.gene87845	MS.gene51799	PPI
MS.gene038757	MS.gene87845	PPI
MS.gene048072	MS.gene87845	PPI
MS.gene87845	MS.gene09023	PPI
MS.gene87845	MS.gene025987	PPI
MS.gene05436	MS.gene87845	PPI
MS.gene87845	MS.gene95960	PPI
MS.gene87845	MS.gene043140	PPI
MS.gene70932	MS.gene87845	PPI
MS.gene047954	MS.gene87845	PPI
MS.gene35175	MS.gene87845	PPI
MS.gene87845	MS.gene03149	PPI
MS.gene87845	MS.gene29321	PPI
MS.gene054652	MS.gene87845	PPI
MS.gene021965	MS.gene87845	PPI
MS.gene055683	MS.gene87845	PPI
MS.gene042614	MS.gene87845	PPI
MS.gene41643	MS.gene87845	PPI
MS.gene26844	MS.gene87845	PPI
MS.gene87845	MS.gene015093	PPI
MS.gene021647	MS.gene87845	PPI
MS.gene66651	MS.gene87845	PPI
MS.gene96109	MS.gene87845	PPI
MS.gene87845	MS.gene24278	PPI
MS.gene87845	MS.gene000379	PPI
MS.gene35202	MS.gene87845	PPI
MS.gene87845	MS.gene34618	PPI
MS.gene048706	MS.gene87845	PPI
MS.gene051449	MS.gene87845	PPI
MS.gene87845	MS.gene59460	PPI
MS.gene009121	MS.gene87845	PPI
MS.gene87845	MS.gene08713	PPI
MS.gene48477	MS.gene87845	PPI
MS.gene51804	MS.gene87845	PPI
MS.gene87845	MS.gene09517	PPI
MS.gene36069	MS.gene87845	PPI
MS.gene30136	MS.gene87845	PPI
MS.gene61452	MS.gene87845	PPI
MS.gene008927	MS.gene87845	PPI
MS.gene016102	MS.gene87845	PPI
MS.gene46941	MS.gene87845	PPI
MS.gene045614	MS.gene87845	PPI
MS.gene96541	MS.gene87845	PPI
MS.gene049561	MS.gene87845	PPI
MS.gene000634	MS.gene87845	PPI
MS.gene46948	MS.gene87845	PPI
MS.gene87845	MS.gene44144	PPI
MS.gene025873	MS.gene87845	PPI
MS.gene012019	MS.gene87845	PPI
MS.gene06164	MS.gene87845	PPI
MS.gene013676	MS.gene87845	PPI
MS.gene05358	MS.gene87845	PPI
MS.gene95561	MS.gene87845	PPI
MS.gene87845	MS.gene027169	PPI
MS.gene87845	MS.gene004417	PPI
MS.gene074257	MS.gene87845	PPI
MS.gene87845	MS.gene019177	PPI
MS.gene065627	MS.gene87845	PPI
MS.gene018745	MS.gene87845	PPI
MS.gene000629	MS.gene87845	PPI
MS.gene65546	MS.gene87845	PPI
MS.gene059269	MS.gene87845	PPI
MS.gene000506	MS.gene87845	PPI
MS.gene87845	MS.gene009213	PPI
MS.gene87845	MS.gene024297	PPI
MS.gene05319	MS.gene87845	PPI
MS.gene87845	MS.gene003264	PPI
MS.gene038098	MS.gene87845	PPI
MS.gene56991	MS.gene87845	PPI
MS.gene87845	MS.gene37537	PPI
MS.gene85538	MS.gene87845	PPI
MS.gene87845	MS.gene069786	PPI
MS.gene22111	MS.gene87845	PPI
MS.gene87845	MS.gene42010	PPI
MS.gene28545	MS.gene87845	PPI
MS.gene28718	MS.gene87845	PPI
MS.gene87845	MS.gene27329	PPI
MS.gene055560	MS.gene87845	PPI
MS.gene87845	MS.gene41393	PPI
MS.gene87845	MS.gene89815	PPI
MS.gene064966	MS.gene87845	PPI
MS.gene87845	MS.gene016632	PPI
MS.gene87845	MS.gene018474	PPI
MS.gene002590	MS.gene87845	PPI
MS.gene87845	MS.gene40469	PPI
MS.gene87845	MS.gene96163	PPI
MS.gene98616	MS.gene87845	PPI
MS.gene40828	MS.gene87845	PPI
MS.gene43924	MS.gene87845	PPI
MS.gene046089	MS.gene87845	PPI
MS.gene036423	MS.gene87845	PPI
MS.gene87845	MS.gene34463	PPI
MS.gene013644	MS.gene87845	PPI
MS.gene80362	MS.gene87845	PPI
MS.gene72520	MS.gene87845	PPI
MS.gene013193	MS.gene87845	PPI
MS.gene87845	MS.gene61499	PPI
MS.gene87845	MS.gene81194	PPI
MS.gene63743	MS.gene87845	PPI
MS.gene87845	MS.gene060773	PPI
MS.gene87845	MS.gene038394	PPI
MS.gene63150	MS.gene87845	PPI
MS.gene045875	MS.gene87845	PPI
MS.gene87845	MS.gene019001	PPI
MS.gene87845	MS.gene53904	PPI
MS.gene87845	MS.gene24070	PPI
MS.gene87845	MS.gene73261	PPI
MS.gene008861	MS.gene87845	PPI
MS.gene024405	MS.gene87845	PPI
MS.gene73261	MS.gene87845	PPI
MS.gene012748	MS.gene87845	PPI
MS.gene024297	MS.gene87845	PPI
MS.gene87845	MS.gene025675	PPI
MS.gene83979	MS.gene87845	PPI
MS.gene87845	MS.gene055546	PPI
MS.gene87845	MS.gene55209	PPI
MS.gene85295	MS.gene87845	PPI
MS.gene47792	MS.gene87845	PPI
MS.gene98471	MS.gene87845	PPI
MS.gene028714	MS.gene87845	PPI
MS.gene049610	MS.gene87845	PPI
MS.gene038676	MS.gene87845	PPI
MS.gene060718	MS.gene87845	PPI
MS.gene87845	MS.gene022929	PPI
MS.gene042651	MS.gene87845	PPI
MS.gene93396	MS.gene87845	PPI
MS.gene87845	MS.gene068629	PPI
MS.gene05576	MS.gene87845	PPI
MS.gene87845	MS.gene009781	PPI
MS.gene52349	MS.gene87845	PPI
MS.gene05231	MS.gene87845	PPI
MS.gene98467	MS.gene87845	PPI
MS.gene87845	MS.gene048342	PPI
MS.gene010656	MS.gene87845	PPI
MS.gene037049	MS.gene87845	PPI
MS.gene87845	MS.gene069624	PPI
MS.gene034643	MS.gene87845	PPI
MS.gene40469	MS.gene87845	PPI
MS.gene041264	MS.gene87845	PPI
MS.gene41979	MS.gene87845	PPI
MS.gene037634	MS.gene87845	PPI
MS.gene87845	MS.gene21066	PPI
MS.gene024522	MS.gene87845	PPI
MS.gene87845	MS.gene013890	PPI
MS.gene87845	MS.gene98127	PPI
MS.gene64130	MS.gene87845	PPI
MS.gene050679	MS.gene87845	PPI
MS.gene87845	MS.gene028714	PPI
MS.gene20246	MS.gene87845	PPI
MS.gene87845	MS.gene048706	PPI
MS.gene055223	MS.gene87845	PPI
MS.gene016719	MS.gene87845	PPI
MS.gene87845	MS.gene69586	PPI
MS.gene87845	MS.gene45083	PPI
MS.gene87845	MS.gene049561	PPI
MS.gene071784	MS.gene87845	PPI
MS.gene87845	MS.gene008658	PPI
MS.gene053747	MS.gene87845	PPI
MS.gene061579	MS.gene87845	PPI
MS.gene87845	MS.gene49057	PPI
MS.gene76420	MS.gene87845	PPI
MS.gene071320	MS.gene87845	PPI
MS.gene65471	MS.gene87845	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87845.t1	MTR_3g053920	58.790	347	75	4	1	346	1	280	5.94e-130	378
MS.gene87845.t1	MTR_2g061500	87.597	129	16	0	1	129	1	129	7.58e-79	242
MS.gene87845.t1	MTR_4g103530	84.211	95	15	0	55	149	1	95	6.43e-55	182
MS.gene87845.t1	MTR_7g011710	68.235	85	27	0	55	139	1	85	4.44e-37	134
MS.gene87845.t1	MTR_6g033215	35.260	173	110	1	29	199	122	294	4.91e-34	130
MS.gene87845.t1	MTR_0010s0370	36.158	177	103	3	8	177	4	177	1.15e-30	117
MS.gene87845.t1	MTR_0010s0350	36.158	177	103	3	8	177	4	177	1.15e-30	117
MS.gene87845.t1	MTR_8g463110	56.140	114	48	1	289	400	1	114	1.95e-24	107
MS.gene87845.t1	MTR_8g463110	66.176	68	23	0	400	467	194	261	9.49e-24	105
MS.gene87845.t1	MTR_6g034215	64.706	68	24	0	400	467	198	265	2.03e-20	92.4
MS.gene87845.t1	MTR_4g020630	57.353	68	29	0	400	467	130	197	1.57e-19	91.7
MS.gene87845.t1	MTR_1g100687	53.731	67	31	0	401	467	133	199	4.93e-18	83.6
MS.gene87845.t1	MTR_1g036720	33.010	103	69	0	11	113	9	111	8.39e-13	65.1
MS.gene87845.t1	MTR_6g009010	44.615	65	36	0	396	460	197	261	4.63e-12	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87845.t1	AT2G17920	25.806	217	147	6	8	216	9	219	3.39e-17	82.8
MS.gene87845.t1	AT3G57587	28.169	142	98	2	77	216	80	219	8.52e-16	79.3
MS.gene87845.t1	AT3G31430	28.230	209	142	5	11	215	107	311	1.53e-15	78.2
MS.gene87845.t1	AT5G36228	27.179	195	134	4	36	226	39	229	2.20e-15	77.8
MS.gene87845.t1	AT2G13450	26.776	183	128	4	36	215	39	218	4.01e-15	77.4
MS.gene87845.t1	AT2G41590	28.169	142	98	2	77	216	80	219	4.09e-15	77.0
MS.gene87845.t1	AT4G02000	27.941	136	94	2	83	216	6	139	2.23e-13	71.2
MS.gene87845.t1	AT5G25200	25.137	183	133	2	36	216	39	219	2.49e-13	71.6
MS.gene87845.t1	AT5G18636	25.137	183	133	2	36	216	39	219	2.70e-13	71.6
MS.gene87845.t1	AT2G02103	25.683	183	132	2	36	216	39	219	3.49e-13	71.6
MS.gene87845.t1	AT3G47920	27.465	142	99	2	77	216	9	148	4.51e-13	70.1

Find 131 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCGCACATGAAGATAATT+TGG	0.155536	8.4:-62063068	None:intergenic
ATTTGCTGCTGATGGGATTA+TGG	0.226831	8.4:+62062101	MS.gene87845:CDS
CATCAACCTTATGGTGAAAT+TGG	0.236408	8.4:-62061180	None:intergenic
CAGGTCCATGGTTTACCATT+TGG	0.298080	8.4:+62061322	MS.gene87845:CDS
AGGACTTTGTTACTAATTCT+TGG	0.298190	8.4:+62063017	MS.gene87845:CDS
AAGCCTTCATCTCAAAGAAT+TGG	0.310102	8.4:+62061652	MS.gene87845:CDS
CCTAAGGTAGGTCAAGGTAT+AGG	0.317348	8.4:+62061355	MS.gene87845:CDS
TGCTACTAACAGGTGGTTAC+AGG	0.322033	8.4:+62061681	MS.gene87845:CDS
ATTATGGGAAATGTTGTAAT+AGG	0.327741	8.4:+62062117	MS.gene87845:CDS
AGACATACGCTCGCTGAAAT+AGG	0.339311	8.4:-62061095	None:intergenic
TCATATCGAGTTCTGGATTC+AGG	0.340405	8.4:+62061303	MS.gene87845:CDS
TCGTAGCGAAAATGACGTTT+AGG	0.342945	8.4:-62062965	None:intergenic
AGGTGAGACCGTCAAGATTC+AGG	0.350710	8.4:-62060964	None:intergenic
CGTTGAATCATATCGAGTTC+TGG	0.362336	8.4:+62061296	MS.gene87845:CDS
CTGATGATGGAGTCCGTAGA+TGG	0.370425	8.4:+62061617	MS.gene87845:CDS
GTTCCAATTCTTTGAGATGA+AGG	0.382349	8.4:-62061655	None:intergenic
GCAGTGTTAGCGCGAGCAGC+AGG	0.384484	8.4:-62061754	None:intergenic
CCTGACCGTCCATTGGTGCT+TGG	0.385983	8.4:+62061970	MS.gene87845:CDS
GCTTGGATTACCTGCTGTTC+CGG	0.391686	8.4:+62061987	MS.gene87845:CDS
TGCTGCCCAAAATGCTGTAT+TGG	0.392978	8.4:+62061861	MS.gene87845:CDS
CTAAGGTAGGTCAAGGTATA+GGG	0.394084	8.4:+62061356	MS.gene87845:CDS
TGTCACAGGTGTGGACTGTT+GGG	0.400708	8.4:+62061553	MS.gene87845:CDS
TAGCTCTTACATGAGACTTA+AGG	0.402604	8.4:+62061426	MS.gene87845:CDS
CACGGGCAGCATCAACCTTA+TGG	0.405332	8.4:-62061189	None:intergenic
CTTGTTCCAATTTCACCATA+AGG	0.405419	8.4:+62061174	MS.gene87845:CDS
CGCTTGGAAGCCAGGCAAAA+AGG	0.410176	8.4:+62061120	MS.gene87845:CDS
GATTCAAGCTCGAACAAGTC+TGG	0.410982	8.4:-62061595	None:intergenic
TTTGCTGCTGATGGGATTAT+GGG	0.413203	8.4:+62062102	MS.gene87845:CDS
TGATTCAACGGGACAAAACT+TGG	0.415783	8.4:-62061283	None:intergenic
TTGTATCTCAGCATCGGATT+CGG	0.417137	8.4:-62062016	None:intergenic
TTGGAACAGCTGCTACTAAC+AGG	0.423746	8.4:+62061671	MS.gene87845:CDS
GGTGCAACATTGACAAGAAT+AGG	0.430171	8.4:-62062923	None:intergenic
ATGTCTCGCGCTTGGAAGCC+AGG	0.433996	8.4:+62061112	MS.gene87845:CDS
GCATCGGATTCGGTTGAAAC+CGG	0.435990	8.4:-62062006	None:intergenic
GTAGCGATCAGCAACAGATT+TGG	0.441459	8.4:-62061152	None:intergenic
ACACGCCACTCTTGTTTCAA+TGG	0.441938	8.4:-62061469	None:intergenic
TGGCGTGTTCGTGCTACTAA+TGG	0.442394	8.4:+62061484	MS.gene87845:CDS
GCATCTAGAACCGGGGTTCA+AGG	0.444158	8.4:+62062997	MS.gene87845:CDS
AAATGCGTGGCATCTAGAAC+CGG	0.444265	8.4:+62062988	MS.gene87845:CDS
CCTATACCTTGACCTACCTT+AGG	0.447996	8.4:-62061355	None:intergenic
ACAAAACTTGGGACGACACC+AGG	0.448728	8.4:-62061271	None:intergenic
TGAGATGAAGGCTTTAAATA+CGG	0.453298	8.4:-62061643	None:intergenic
GACCTATGAGACAGTGTTCA+AGG	0.454398	8.4:-62061042	None:intergenic
TGGTGCTACAGGCGAAGATC+AGG	0.457205	8.4:+62063177	MS.gene87845:intron
CTTTGTTACTAATTCTTGGC+AGG	0.457702	8.4:+62063021	MS.gene87845:CDS
TTGTCACAGGTGTGGACTGT+TGG	0.459654	8.4:+62061552	MS.gene87845:CDS
GTCCTTGAACACTGTCTCAT+AGG	0.463466	8.4:+62061040	MS.gene87845:CDS
TCCGCACATGAAGATAATTT+GGG	0.464716	8.4:-62063067	None:intergenic
CCAAGCACCAATGGACGGTC+AGG	0.469030	8.4:-62061970	None:intergenic
TGCGGCCATCATAAGCTTTG+AGG	0.469996	8.4:-62061393	None:intergenic
GCTGCCCGTGTTCTTGATGA+AGG	0.477877	8.4:+62061202	MS.gene87845:CDS
AGAACTCGATATGATTCAAC+GGG	0.480591	8.4:-62061294	None:intergenic
TGCGACATAGAGACAGGAAT+AGG	0.483601	8.4:-62061706	None:intergenic
CTGGTTCAGACTCAAGGTTA+AGG	0.485928	8.4:-62061006	None:intergenic
TTCCTCTTGTATCTCAGCAT+CGG	0.486377	8.4:-62062022	None:intergenic
TTCGAGCTTGAATCTGATGA+TGG	0.494215	8.4:+62061604	MS.gene87845:CDS
GCGACATAGAGACAGGAATA+GGG	0.501118	8.4:-62061705	None:intergenic
TGTTGTACCTGTGGAGGAAG+AGG	0.502378	8.4:-62061932	None:intergenic
GTGGTTCGGATTTGAAGAAA+AGG	0.503559	8.4:+62062046	MS.gene87845:CDS
TCGGTTGAAACCGGAACAGC+AGG	0.506727	8.4:-62061997	None:intergenic
CTGCTGCCCTCTTCCTCCAC+AGG	0.506951	8.4:+62061925	MS.gene87845:CDS
GGACGACACCAGGGGAAATA+CGG	0.507266	8.4:-62061261	None:intergenic
GCGGCCATCATAAGCTTTGA+GGG	0.507959	8.4:-62061392	None:intergenic
GTTATGGACCGTATTTCCCC+TGG	0.508743	8.4:+62061253	MS.gene87845:CDS
CCCACCAACCTGAATCTTGA+CGG	0.509838	8.4:+62060956	MS.gene87845:CDS
GAAGAAAAGGAAGCGTATGT+TGG	0.516569	8.4:+62062059	MS.gene87845:CDS
TTAGGTTGAATGAAACCAAA+TGG	0.516817	8.4:-62061337	None:intergenic
CTGTCTCATAGGTCGAGTCC+TGG	0.520328	8.4:+62061051	MS.gene87845:CDS
TTAAATACGGTCCCCATCTA+CGG	0.521489	8.4:-62061630	None:intergenic
GTGCAACATTGACAAGAATA+GGG	0.522667	8.4:-62062922	None:intergenic
CATGCTTCCTGACCGTCCAT+TGG	0.524035	8.4:+62061963	MS.gene87845:CDS
ACCGTCAAGATTCAGGTTGG+TGG	0.526969	8.4:-62060957	None:intergenic
ATTTGGTTTCATTCAACCTA+AGG	0.529018	8.4:+62061339	MS.gene87845:CDS
GAGTTATCATTTGCTGCTGA+TGG	0.529294	8.4:+62062093	MS.gene87845:CDS
TGATGATGGAGTCCGTAGAT+GGG	0.531244	8.4:+62061618	MS.gene87845:CDS
GCAGCAGCATTGAGAGGAGA+TGG	0.536233	8.4:-62061730	None:intergenic
GATACAAGAGGAAAGTGGTT+CGG	0.537665	8.4:+62062032	MS.gene87845:CDS
GCGAGCGTATGTCTCGCGCT+TGG	0.538462	8.4:+62061104	MS.gene87845:CDS
GCTCGCGCTAACACTGCTGA+AGG	0.542242	8.4:+62061760	MS.gene87845:CDS
CGTTTAGGAATCTGTAACCG+AGG	0.548525	8.4:-62062950	None:intergenic
GATGGCTGCGACATAGAGAC+AGG	0.549076	8.4:-62061712	None:intergenic
GTGTTCTTGATGAAGGGCCA+TGG	0.550277	8.4:+62061209	MS.gene87845:CDS
GGTACCCTCAAAGCTTATGA+TGG	0.559787	8.4:+62061388	MS.gene87845:CDS
ATAGGGTAATGATCGGAAGC+AGG	0.563188	8.4:-62062905	None:intergenic
CCGTCAAGATTCAGGTTGGT+GGG	0.564705	8.4:-62060956	None:intergenic
ATTGAATCTCGCGATCAGCC+AGG	0.565008	8.4:-62061069	None:intergenic
GGTTTCATTCAACCTAAGGT+AGG	0.570176	8.4:+62061343	MS.gene87845:CDS
GCTAGAGGCTGTTGTACCTG+TGG	0.570726	8.4:-62061941	None:intergenic
GAGTTCTGGATTCAGGTCCA+TGG	0.571214	8.4:+62061310	MS.gene87845:CDS
GTCACAGGTGTGGACTGTTG+GGG	0.571487	8.4:+62061554	MS.gene87845:CDS
TCCCAAATTATCTTCATGTG+CGG	0.572394	8.4:+62063066	MS.gene87845:CDS
GTAACAAAGTCCTTGAACCC+CGG	0.574550	8.4:-62063007	None:intergenic
AGTTATCATTTGCTGCTGAT+GGG	0.576161	8.4:+62062094	MS.gene87845:CDS
ATTCAACCTAAGGTAGGTCA+AGG	0.576603	8.4:+62061349	MS.gene87845:CDS
GGAATCTGTAACCGAGGTGT+AGG	0.578721	8.4:-62062944	None:intergenic
AATGCGTGGCATCTAGAACC+GGG	0.585894	8.4:+62062989	MS.gene87845:CDS
GACAAGAATAGGGTAATGAT+CGG	0.586157	8.4:-62062912	None:intergenic
GCAGGAGCAGCAGCATTGAG+AGG	0.588766	8.4:-62061736	None:intergenic
GATTCAACGGGACAAAACTT+GGG	0.588972	8.4:-62061282	None:intergenic
GGTCTCACCTTGTCTGAGGA+AGG	0.589254	8.4:+62060977	MS.gene87845:CDS
GCTAACACTGCTGAAGGCAA+TGG	0.591118	8.4:+62061766	MS.gene87845:CDS
CAAATTATCTTCATGTGCGG+AGG	0.592639	8.4:+62063069	MS.gene87845:CDS
ATGCGTGGCATCTAGAACCG+GGG	0.598375	8.4:+62062990	MS.gene87845:CDS
GTTGTACCTGTGGAGGAAGA+GGG	0.604958	8.4:-62061931	None:intergenic
TGACGGTCTCACCTTGTCTG+AGG	0.605230	8.4:+62060973	MS.gene87845:CDS
TCTCATGTAAGAGCTATGAA+TGG	0.614120	8.4:-62061419	None:intergenic
TGAAACCAAATGGTAAACCA+TGG	0.615949	8.4:-62061327	None:intergenic
ATGGCCCTTCATCAAGAACA+CGG	0.617686	8.4:-62061207	None:intergenic
CAGAACTCGATATGATTCAA+CGG	0.618989	8.4:-62061295	None:intergenic
TGAAAATTATCATAAAGCCA+TGG	0.620312	8.4:-62061226	None:intergenic
GATGATGGAGTCCGTAGATG+GGG	0.623710	8.4:+62061619	MS.gene87845:CDS
GACGAGACCTTCCTCAGACA+AGG	0.633356	8.4:-62060984	None:intergenic
GCAGGTAATCCAAGCACCAA+TGG	0.634137	8.4:-62061979	None:intergenic
ATCCGATGCTGAGATACAAG+AGG	0.634910	8.4:+62062020	MS.gene87845:CDS
AGAGGCTGTTGTACCTGTGG+AGG	0.640336	8.4:-62061938	None:intergenic
GCTGAGATACAAGAGGAAAG+TGG	0.644430	8.4:+62062027	MS.gene87845:CDS
CTGTTCCATTGAAACAAGAG+TGG	0.649479	8.4:+62061464	MS.gene87845:CDS
CTGCCCGTGTTCTTGATGAA+GGG	0.649758	8.4:+62061203	MS.gene87845:CDS
GAGACCGTCAAGATTCAGGT+TGG	0.650330	8.4:-62060960	None:intergenic
TGGCCCTTCATCAAGAACAC+GGG	0.651409	8.4:-62061206	None:intergenic
ACGGTCAGGAAGCATGCTAG+AGG	0.652703	8.4:-62061956	None:intergenic
CAAAACTTGGGACGACACCA+GGG	0.654153	8.4:-62061270	None:intergenic
TCAATGTTGCACCTACACCT+CGG	0.657222	8.4:+62062933	MS.gene87845:CDS
TGCTACTGTTGAGACTCTTG+TGG	0.660371	8.4:+62062880	MS.gene87845:intron
GTAATCCAAGCACCAATGGA+CGG	0.661163	8.4:-62061975	None:intergenic
CAAATGATAACTCATTCTGA+CGG	0.677210	8.4:-62062083	None:intergenic
AAGAGCTATGAATGGTGTTG+CGG	0.687030	8.4:-62061411	None:intergenic
AAAACTTGGGACGACACCAG+GGG	0.689747	8.4:-62061269	None:intergenic
GAACAGCTGCTACTAACAGG+TGG	0.694349	8.4:+62061674	MS.gene87845:CDS
GAGACTGAAAAGCCATCATG+CGG	0.695467	8.4:-62061810	None:intergenic
ACTGTTGGGGCATACTGACA+AGG	0.735140	8.4:+62061567	MS.gene87845:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGATAATTTTCATATTGTTA+TGG	+	chr8.4:62061237-62061256	MS.gene87845:CDS	15.0%
!!	TAACAATTTCAACAGTAATA+TGG	-	chr8.4:62062508-62062527	None:intergenic	20.0%
!!!	CAATGTCTTTTTTTAATTTG+TGG	-	chr8.4:62063123-62063142	None:intergenic	20.0%
!	ACCTTTAAGTATGAAAAACT+TGG	+	chr8.4:62061523-62061542	MS.gene87845:CDS	25.0%
!	AGATATCATTAAAATCTCCT+AGG	-	chr8.4:62062652-62062671	None:intergenic	25.0%
!	ATTATGGGAAATGTTGTAAT+AGG	+	chr8.4:62062117-62062136	MS.gene87845:CDS	25.0%
!	GAAATTGTTAACAATACTCT+TGG	+	chr8.4:62062518-62062537	MS.gene87845:intron	25.0%
!	TGAAAATTATCATAAAGCCA+TGG	-	chr8.4:62061229-62061248	None:intergenic	25.0%
!!	ACCAAGTTTTTCATACTTAA+AGG	-	chr8.4:62061527-62061546	None:intergenic	25.0%
!!	TTAACAATACTCTTGGTAAT+TGG	+	chr8.4:62062525-62062544	MS.gene87845:intron	25.0%
!!!	GGAGTTCTTCAATTTTATTT+CGG	-	chr8.4:62062370-62062389	None:intergenic	25.0%
	AATCGTAACCAAGCAAAAAA+CGG	-	chr8.4:62062391-62062410	None:intergenic	30.0%
	AGATGATGAACAAGTTGTAA+TGG	+	chr8.4:62062194-62062213	MS.gene87845:intron	30.0%
	AGGACTTTGTTACTAATTCT+TGG	+	chr8.4:62063017-62063036	MS.gene87845:CDS	30.0%
	CAAATGATAACTCATTCTGA+CGG	-	chr8.4:62062086-62062105	None:intergenic	30.0%
	TACACTTGGTTTAAAAGTCT+TGG	+	chr8.4:62062785-62062804	MS.gene87845:intron	30.0%
	TCACTTAGGAATAAAAGATC+CGG	-	chr8.4:62062335-62062354	None:intergenic	30.0%
!	ACAACTTGTTCATCATCTAT+AGG	-	chr8.4:62062192-62062211	None:intergenic	30.0%
!	AGACTTTTAAACCAAGTGTA+CGG	-	chr8.4:62062785-62062804	None:intergenic	30.0%
!	ATTTGGTTTCATTCAACCTA+AGG	+	chr8.4:62061339-62061358	MS.gene87845:CDS	30.0%
!	CAACTTTCTTCTCAATTTTC+AGG	+	chr8.4:62062614-62062633	MS.gene87845:intron	30.0%
!	TTTAGCCAATACAGCATTTT+GGG	-	chr8.4:62061869-62061888	None:intergenic	30.0%
!	TTTTAGCCAATACAGCATTT+TGG	-	chr8.4:62061870-62061889	None:intergenic	30.0%
!!	AATGCTGTATTGGCTAAAAA+AGG	+	chr8.4:62061871-62061890	MS.gene87845:CDS	30.0%
!!	GTATTGGCTAAAAAAGGTAA+TGG	+	chr8.4:62061877-62061896	MS.gene87845:CDS	30.0%
!!	TGAGATGAAGGCTTTAAATA+CGG	-	chr8.4:62061646-62061665	None:intergenic	30.0%
!!	TTAGGTTGAATGAAACCAAA+TGG	-	chr8.4:62061340-62061359	None:intergenic	30.0%
!!!	AACTTGGTATTTTTTGTCAC+AGG	+	chr8.4:62061539-62061558	MS.gene87845:CDS	30.0%
	AAGCCTTCATCTCAAAGAAT+TGG	+	chr8.4:62061652-62061671	MS.gene87845:CDS	35.0%
	AGAACTCGATATGATTCAAC+GGG	-	chr8.4:62061297-62061316	None:intergenic	35.0%
	ATAGGATCCAATGTTAGTCA+TGG	+	chr8.4:62062135-62062154	MS.gene87845:intron	35.0%
	ATTAGGATAGCCATAATAGC+CGG	-	chr8.4:62062558-62062577	None:intergenic	35.0%
	CAGAACTCGATATGATTCAA+CGG	-	chr8.4:62061298-62061317	None:intergenic	35.0%
	CATCAACCTTATGGTGAAAT+TGG	-	chr8.4:62061183-62061202	None:intergenic	35.0%
	CTTAGATGCAAGTGAGAAAA+GGG	+	chr8.4:62062670-62062689	MS.gene87845:intron	35.0%
	CTTGTTCCAATTTCACCATA+AGG	+	chr8.4:62061174-62061193	MS.gene87845:CDS	35.0%
	CTTTGTTACTAATTCTTGGC+AGG	+	chr8.4:62063021-62063040	MS.gene87845:CDS	35.0%
	GACAAGAATAGGGTAATGAT+CGG	-	chr8.4:62062915-62062934	None:intergenic	35.0%
	GATGATATGTATGTAACTGC+TGG	+	chr8.4:62062165-62062184	MS.gene87845:intron	35.0%
	GCAAACAATTACAAGAGACA+CGG	+	chr8.4:62063152-62063171	MS.gene87845:intron	35.0%
	GTGCAACATTGACAAGAATA+GGG	-	chr8.4:62062925-62062944	None:intergenic	35.0%
	GTGCTCTTGCAAATGATTTA+TGG	+	chr8.4:62062840-62062859	MS.gene87845:intron	35.0%
	TAGCTCTTACATGAGACTTA+AGG	+	chr8.4:62061426-62061445	MS.gene87845:CDS	35.0%
	TAGGATCCAATGTTAGTCAT+GGG	+	chr8.4:62062136-62062155	MS.gene87845:intron	35.0%
	TATTATGGCTATCCTAATGG+AGG	+	chr8.4:62062560-62062579	MS.gene87845:intron	35.0%
	TCCCAAATTATCTTCATGTG+CGG	+	chr8.4:62063066-62063085	MS.gene87845:CDS	35.0%
	TCCGCACATGAAGATAATTT+GGG	-	chr8.4:62063070-62063089	None:intergenic	35.0%
	TCTCATGTAAGAGCTATGAA+TGG	-	chr8.4:62061422-62061441	None:intergenic	35.0%
	TCTTAGATGCAAGTGAGAAA+AGG	+	chr8.4:62062669-62062688	MS.gene87845:intron	35.0%
	TGAAACCAAATGGTAAACCA+TGG	-	chr8.4:62061330-62061349	None:intergenic	35.0%
	TTAGATGCAAGTGAGAAAAG+GGG	+	chr8.4:62062671-62062690	MS.gene87845:intron	35.0%
	TTGTCGAAGAAAATTCCAAG+CGG	-	chr8.4:62062597-62062616	None:intergenic	35.0%
!	AGTTATCATTTGCTGCTGAT+GGG	+	chr8.4:62062094-62062113	MS.gene87845:CDS	35.0%
!	TAATTTTCATGACCGCATGA+TGG	+	chr8.4:62061798-62061817	MS.gene87845:CDS	35.0%
!!	GTTCCAATTCTTTGAGATGA+AGG	-	chr8.4:62061658-62061677	None:intergenic	35.0%
	AAGCCATTGATAAGCCATTG+GGG	-	chr8.4:62062710-62062729	None:intergenic	40.0%
	AGGATCCAATGTTAGTCATG+GGG	+	chr8.4:62062137-62062156	MS.gene87845:intron	40.0%
	ATTCAACCTAAGGTAGGTCA+AGG	+	chr8.4:62061349-62061368	MS.gene87845:CDS	40.0%
	CAAATTATCTTCATGTGCGG+AGG	+	chr8.4:62063069-62063088	MS.gene87845:CDS	40.0%
	CCAAAATAAAGCTAAACCCC+CGG	-	chr8.4:62062444-62062463	None:intergenic	40.0%
	CGTTGAATCATATCGAGTTC+TGG	+	chr8.4:62061296-62061315	MS.gene87845:CDS	40.0%
	CTAAGGTAGGTCAAGGTATA+GGG	+	chr8.4:62061356-62061375	MS.gene87845:CDS	40.0%
	CTCCGCACATGAAGATAATT+TGG	-	chr8.4:62063071-62063090	None:intergenic	40.0%
	CTGTTCCATTGAAACAAGAG+TGG	+	chr8.4:62061464-62061483	MS.gene87845:CDS	40.0%
	GAAGCCATTGATAAGCCATT+GGG	-	chr8.4:62062711-62062730	None:intergenic	40.0%
	GATACAAGAGGAAAGTGGTT+CGG	+	chr8.4:62062032-62062051	MS.gene87845:CDS	40.0%
	GATGAACAAGTTGTAATGGC+AGG	+	chr8.4:62062198-62062217	MS.gene87845:intron	40.0%
	GATTCAACGGGACAAAACTT+GGG	-	chr8.4:62061285-62061304	None:intergenic	40.0%
	GGCTATTATGGCTATCCTAA+TGG	+	chr8.4:62062557-62062576	MS.gene87845:intron	40.0%
	GGTGCAACATTGACAAGAAT+AGG	-	chr8.4:62062926-62062945	None:intergenic	40.0%
	GGTTTCATTCAACCTAAGGT+AGG	+	chr8.4:62061343-62061362	MS.gene87845:CDS	40.0%
	TCGTAGCGAAAATGACGTTT+AGG	-	chr8.4:62062968-62062987	None:intergenic	40.0%
	TGATTCAACGGGACAAAACT+TGG	-	chr8.4:62061286-62061305	None:intergenic	40.0%
	TTAAATACGGTCCCCATCTA+CGG	-	chr8.4:62061633-62061652	None:intergenic	40.0%
	TTCCTCTTGTATCTCAGCAT+CGG	-	chr8.4:62062025-62062044	None:intergenic	40.0%
	TTCGAGCTTGAATCTGATGA+TGG	+	chr8.4:62061604-62061623	MS.gene87845:CDS	40.0%
	TTTGCTGCTGATGGGATTAT+GGG	+	chr8.4:62062102-62062121	MS.gene87845:CDS	40.0%
!	AAGAGCTATGAATGGTGTTG+CGG	-	chr8.4:62061414-62061433	None:intergenic	40.0%
!	ATTTGCTGCTGATGGGATTA+TGG	+	chr8.4:62062101-62062120	MS.gene87845:CDS	40.0%
!	ATTTTCGCTACGAAAATGCG+TGG	+	chr8.4:62062975-62062994	MS.gene87845:CDS	40.0%
!	CAAGGTATAGGGAGTTTTCT+AGG	+	chr8.4:62061367-62061386	MS.gene87845:CDS	40.0%
!	GAAGAAAAGGAAGCGTATGT+TGG	+	chr8.4:62062059-62062078	MS.gene87845:CDS	40.0%
!	GACGAAATTTAAGACCCCAA+TGG	+	chr8.4:62062693-62062712	MS.gene87845:intron	40.0%
!	GAGTTATCATTTGCTGCTGA+TGG	+	chr8.4:62062093-62062112	MS.gene87845:CDS	40.0%
!	GTGGTTCGGATTTGAAGAAA+AGG	+	chr8.4:62062046-62062065	MS.gene87845:CDS	40.0%
!	TTGTATCTCAGCATCGGATT+CGG	-	chr8.4:62062019-62062038	None:intergenic	40.0%
!!	TCATATCGAGTTCTGGATTC+AGG	+	chr8.4:62061303-62061322	MS.gene87845:CDS	40.0%
!!!	CATTTTCTGGTTCAGACTCA+AGG	-	chr8.4:62061015-62061034	None:intergenic	40.0%
!!!	CTTCTCAATTTTCAGGTCCA+TGG	+	chr8.4:62062621-62062640	MS.gene87845:intron	40.0%
!!!	GAAGAACTCCGTTTTTTGCT+TGG	+	chr8.4:62062380-62062399	MS.gene87845:intron	40.0%
!!!	GGTATTTTTTGTCACAGGTG+TGG	+	chr8.4:62061544-62061563	MS.gene87845:CDS	40.0%
	AAAGATCCGGATTGAAGTGC+CGG	-	chr8.4:62062322-62062341	None:intergenic	45.0%
	AAATCTCCTAGGATGCACCA+TGG	-	chr8.4:62062641-62062660	None:intergenic	45.0%
	AGACATACGCTCGCTGAAAT+AGG	-	chr8.4:62061098-62061117	None:intergenic	45.0%
	AGACCCCAATGGCTTATCAA+TGG	+	chr8.4:62062704-62062723	MS.gene87845:intron	45.0%
	AGAGTCTCAACAGTAGCATG+CGG	-	chr8.4:62062878-62062897	None:intergenic	45.0%
	AGTGTACGGATAACCTTCGA+CGG	-	chr8.4:62062771-62062790	None:intergenic	45.0%
	ATAGGGTAATGATCGGAAGC+AGG	-	chr8.4:62062908-62062927	None:intergenic	45.0%
	ATCCAATGTTAGTCATGGGG+GGG	+	chr8.4:62062140-62062159	MS.gene87845:intron	45.0%
	ATCCGATGCTGAGATACAAG+AGG	+	chr8.4:62062020-62062039	MS.gene87845:CDS	45.0%
	ATGGCCCTTCATCAAGAACA+CGG	-	chr8.4:62061210-62061229	None:intergenic	45.0%
	CAATTACAAGAGACACGGCT+TGG	+	chr8.4:62063157-62063176	MS.gene87845:intron	45.0%
	CAGGTCCATGGTTTACCATT+TGG	+	chr8.4:62061322-62061341	MS.gene87845:CDS	45.0%
	CCGACCTTAAATACCTAGTC+CGG	+	chr8.4:62062300-62062319	MS.gene87845:intron	45.0%
	CCTAAGGTAGGTCAAGGTAT+AGG	+	chr8.4:62061355-62061374	MS.gene87845:CDS	45.0%
	CCTATACCTTGACCTACCTT+AGG	-	chr8.4:62061358-62061377	None:intergenic	45.0%
	CGTTTAGGAATCTGTAACCG+AGG	-	chr8.4:62062953-62062972	None:intergenic	45.0%
	CTGAATCAAGAACCGCTTGA+CGG	-	chr8.4:62062733-62062752	None:intergenic	45.0%
	CTGGTTCAGACTCAAGGTTA+AGG	-	chr8.4:62061009-62061028	None:intergenic	45.0%
	GACCTATGAGACAGTGTTCA+AGG	-	chr8.4:62061045-62061064	None:intergenic	45.0%
	GAGACTGAAAAGCCATCATG+CGG	-	chr8.4:62061813-62061832	None:intergenic	45.0%
	GATCCAATGTTAGTCATGGG+GGG	+	chr8.4:62062139-62062158	MS.gene87845:intron	45.0%
	GATTCAAGCTCGAACAAGTC+TGG	-	chr8.4:62061598-62061617	None:intergenic	45.0%
	GCGACATAGAGACAGGAATA+GGG	-	chr8.4:62061708-62061727	None:intergenic	45.0%
	GCTACTAGACTCATTCACCT+AGG	-	chr8.4:62062242-62062261	None:intergenic	45.0%
	GCTGAGATACAAGAGGAAAG+TGG	+	chr8.4:62062027-62062046	MS.gene87845:CDS	45.0%
	GGAAGCCATTGATAAGCCAT+TGG	-	chr8.4:62062712-62062731	None:intergenic	45.0%
	GGATCCAATGTTAGTCATGG+GGG	+	chr8.4:62062138-62062157	MS.gene87845:intron	45.0%
	GGTACCCTCAAAGCTTATGA+TGG	+	chr8.4:62061388-62061407	MS.gene87845:CDS	45.0%
	GGTGAATGAGTCTAGTAGCT+TGG	+	chr8.4:62062243-62062262	MS.gene87845:intron	45.0%
	GTAACAAAGTCCTTGAACCC+CGG	-	chr8.4:62063010-62063029	None:intergenic	45.0%
	GTAATCCAAGCACCAATGGA+CGG	-	chr8.4:62061978-62061997	None:intergenic	45.0%
	GTAGCGATCAGCAACAGATT+TGG	-	chr8.4:62061155-62061174	None:intergenic	45.0%
	GTCCTTGAACACTGTCTCAT+AGG	+	chr8.4:62061040-62061059	MS.gene87845:CDS	45.0%
	GTCTAGTAGCTTGGAATTGC+CGG	+	chr8.4:62062252-62062271	MS.gene87845:intron	45.0%
	TATCAATGGCTTCCGTCAAG+CGG	+	chr8.4:62062718-62062737	MS.gene87845:intron	45.0%
	TCAATGTTGCACCTACACCT+CGG	+	chr8.4:62062933-62062952	MS.gene87845:CDS	45.0%
	TCGAAGGTTATCCGTACACT+TGG	+	chr8.4:62062771-62062790	MS.gene87845:intron	45.0%
	TCTAGTAGCTTGGAATTGCC+GGG	+	chr8.4:62062253-62062272	MS.gene87845:intron	45.0%
	TGCGACATAGAGACAGGAAT+AGG	-	chr8.4:62061709-62061728	None:intergenic	45.0%
	TGCTACTAACAGGTGGTTAC+AGG	+	chr8.4:62061681-62061700	MS.gene87845:CDS	45.0%
	TGCTACTGTTGAGACTCTTG+TGG	+	chr8.4:62062880-62062899	MS.gene87845:intron	45.0%
	TGCTGCCCAAAATGCTGTAT+TGG	+	chr8.4:62061861-62061880	MS.gene87845:CDS	45.0%
	TTAAGGTCGGGAATTGCACT+CGG	-	chr8.4:62062290-62062309	None:intergenic	45.0%
	TTGGAACAGCTGCTACTAAC+AGG	+	chr8.4:62061671-62061690	MS.gene87845:CDS	45.0%
!	AAATGCGTGGCATCTAGAAC+CGG	+	chr8.4:62062988-62063007	MS.gene87845:CDS	45.0%
!	AATGGCAGGTTCTGATGATC+AGG	+	chr8.4:62062212-62062231	MS.gene87845:intron	45.0%
!	ACACGCCACTCTTGTTTCAA+TGG	-	chr8.4:62061472-62061491	None:intergenic	45.0%
!	AGTGCCGGACTAGGTATTTA+AGG	-	chr8.4:62062307-62062326	None:intergenic	45.0%
!	CCGGACTAGGTATTTAAGGT+CGG	-	chr8.4:62062303-62062322	None:intergenic	45.0%
!	CGGACTAGGTATTTAAGGTC+GGG	-	chr8.4:62062302-62062321	None:intergenic	45.0%
!	CTTGGTAATTGGCGTGTTAC+CGG	+	chr8.4:62062536-62062555	MS.gene87845:intron	45.0%
!	TCAAGGACAGCAGCATTTTC+TGG	-	chr8.4:62061028-62061047	None:intergenic	45.0%
!!	TGATGATGGAGTCCGTAGAT+GGG	+	chr8.4:62061618-62061637	MS.gene87845:CDS	45.0%
!!!	CCGGGGGTTTAGCTTTATTT+TGG	+	chr8.4:62062441-62062460	MS.gene87845:intron	45.0%
	AAAACTTGGGACGACACCAG+GGG	-	chr8.4:62061272-62061291	None:intergenic	50.0%
	AAGTCTTGGTACTCCACGTG+CGG	+	chr8.4:62062799-62062818	MS.gene87845:intron	50.0%
	ACAAAACTTGGGACGACACC+AGG	-	chr8.4:62061274-62061293	None:intergenic	50.0%
	ACTGTTGGGGCATACTGACA+AGG	+	chr8.4:62061567-62061586	MS.gene87845:CDS	50.0%
	AGGTGAGACCGTCAAGATTC+AGG	-	chr8.4:62060967-62060986	None:intergenic	50.0%
	ATAACCTTCGACGGGAACGT+CGG	-	chr8.4:62062762-62062781	None:intergenic	50.0%
	ATCCCCCCCATGACTAACAT+TGG	-	chr8.4:62062145-62062164	None:intergenic	50.0%
	ATTGAATCTCGCGATCAGCC+AGG	-	chr8.4:62061072-62061091	None:intergenic	50.0%
	CAAAACTTGGGACGACACCA+GGG	-	chr8.4:62061273-62061292	None:intergenic	50.0%
	CCCACCAACCTGAATCTTGA+CGG	+	chr8.4:62060956-62060975	MS.gene87845:CDS	50.0%
	CGTCAAGCGGTTCTTGATTC+AGG	+	chr8.4:62062731-62062750	MS.gene87845:intron	50.0%
	CTAGACTCATTCACCTAGGC+AGG	-	chr8.4:62062238-62062257	None:intergenic	50.0%
	CTAGTAGCTTGGAATTGCCG+GGG	+	chr8.4:62062254-62062273	MS.gene87845:intron	50.0%
	CTGCCCGTGTTCTTGATGAA+GGG	+	chr8.4:62061203-62061222	MS.gene87845:CDS	50.0%
	GAACAGCTGCTACTAACAGG+TGG	+	chr8.4:62061674-62061693	MS.gene87845:CDS	50.0%
	GAGACCGTCAAGATTCAGGT+TGG	-	chr8.4:62060963-62060982	None:intergenic	50.0%
	GAGTCTCAACAGTAGCATGC+GGG	-	chr8.4:62062877-62062896	None:intergenic	50.0%
	GCAGGTAATCCAAGCACCAA+TGG	-	chr8.4:62061982-62062001	None:intergenic	50.0%
	GCTAACACTGCTGAAGGCAA+TGG	+	chr8.4:62061766-62061785	MS.gene87845:CDS	50.0%
	GCTGATCTACGACCTCCATT+AGG	-	chr8.4:62062575-62062594	None:intergenic	50.0%
	GCTTGGATTACCTGCTGTTC+CGG	+	chr8.4:62061987-62062006	MS.gene87845:CDS	50.0%
	GGAATCTGTAACCGAGGTGT+AGG	-	chr8.4:62062947-62062966	None:intergenic	50.0%
	GGTGAGCTAGTGTCTCACTT+AGG	-	chr8.4:62062349-62062368	None:intergenic	50.0%
	GTGTACGGATAACCTTCGAC+GGG	-	chr8.4:62062770-62062789	None:intergenic	50.0%
	GTGTTCTTGATGAAGGGCCA+TGG	+	chr8.4:62061209-62061228	MS.gene87845:CDS	50.0%
	GTTATGGACCGTATTTCCCC+TGG	+	chr8.4:62061253-62061272	MS.gene87845:CDS	50.0%
	GTTGTACCTGTGGAGGAAGA+GGG	-	chr8.4:62061934-62061953	None:intergenic	50.0%
	TAAGGTCGGGAATTGCACTC+GGG	-	chr8.4:62062289-62062308	None:intergenic	50.0%
	TGCGGCCATCATAAGCTTTG+AGG	-	chr8.4:62061396-62061415	None:intergenic	50.0%
	TGGCCCTTCATCAAGAACAC+GGG	-	chr8.4:62061209-62061228	None:intergenic	50.0%
	TGTCTTCATGTAGACCGCAC+CGG	+	chr8.4:62062413-62062432	MS.gene87845:intron	50.0%
	TGTTGTACCTGTGGAGGAAG+AGG	-	chr8.4:62061935-62061954	None:intergenic	50.0%
!	AATGCGTGGCATCTAGAACC+GGG	+	chr8.4:62062989-62063008	MS.gene87845:CDS	50.0%
!	GCATCGGATTCGGTTGAAAC+CGG	-	chr8.4:62062009-62062028	None:intergenic	50.0%
!	GCGGCCATCATAAGCTTTGA+GGG	-	chr8.4:62061395-62061414	None:intergenic	50.0%
!	GTGATCGTGACCTTTTTGCC+TGG	-	chr8.4:62061133-62061152	None:intergenic	50.0%
!	TGGCGTGTTACCGGCTATTA+TGG	+	chr8.4:62062545-62062564	MS.gene87845:intron	50.0%
!	TGGCGTGTTCGTGCTACTAA+TGG	+	chr8.4:62061484-62061503	MS.gene87845:CDS	50.0%
!	TGTCACAGGTGTGGACTGTT+GGG	+	chr8.4:62061553-62061572	MS.gene87845:CDS	50.0%
!	TTGTCACAGGTGTGGACTGT+TGG	+	chr8.4:62061552-62061571	MS.gene87845:CDS	50.0%
!!	ACCGTCAAGATTCAGGTTGG+TGG	-	chr8.4:62060960-62060979	None:intergenic	50.0%
!!	CCGTCAAGATTCAGGTTGGT+GGG	-	chr8.4:62060959-62060978	None:intergenic	50.0%
!!	CTGATGATGGAGTCCGTAGA+TGG	+	chr8.4:62061617-62061636	MS.gene87845:CDS	50.0%
!!	GAGTTCTGGATTCAGGTCCA+TGG	+	chr8.4:62061310-62061329	MS.gene87845:CDS	50.0%
!!	GATGATGGAGTCCGTAGATG+GGG	+	chr8.4:62061619-62061638	MS.gene87845:CDS	50.0%
	AACCTTTCTTCCACCGCACG+TGG	-	chr8.4:62062815-62062834	None:intergenic	55.0%
	AGAGGCTGTTGTACCTGTGG+AGG	-	chr8.4:62061941-62061960	None:intergenic	55.0%
	AGCTAAACCCCCGGATCTAC+CGG	-	chr8.4:62062435-62062454	None:intergenic	55.0%
	CACGGGCAGCATCAACCTTA+TGG	-	chr8.4:62061192-62061211	None:intergenic	55.0%
	CATGCTTCCTGACCGTCCAT+TGG	+	chr8.4:62061963-62061982	MS.gene87845:CDS	55.0%
	CCTAGTCCGGCACTTCAATC+CGG	+	chr8.4:62062313-62062332	MS.gene87845:intron	55.0%
	CGCTTGGAAGCCAGGCAAAA+AGG	+	chr8.4:62061120-62061139	MS.gene87845:CDS	55.0%
	CTGTCTCATAGGTCGAGTCC+TGG	+	chr8.4:62061051-62061070	MS.gene87845:CDS	55.0%
	GACGAGACCTTCCTCAGACA+AGG	-	chr8.4:62060987-62061006	None:intergenic	55.0%
	GATGGCTGCGACATAGAGAC+AGG	-	chr8.4:62061715-62061734	None:intergenic	55.0%
	GCTAGAGGCTGTTGTACCTG+TGG	-	chr8.4:62061944-62061963	None:intergenic	55.0%
	GCTGCCCGTGTTCTTGATGA+AGG	+	chr8.4:62061202-62061221	MS.gene87845:CDS	55.0%
	GGACGACACCAGGGGAAATA+CGG	-	chr8.4:62061264-62061283	None:intergenic	55.0%
	GGTCTCACCTTGTCTGAGGA+AGG	+	chr8.4:62060977-62060996	MS.gene87845:CDS	55.0%
	TCAGGTCCATGGTGCATCCT+AGG	+	chr8.4:62062632-62062651	MS.gene87845:intron	55.0%
	TCGGTTGAAACCGGAACAGC+AGG	-	chr8.4:62062000-62062019	None:intergenic	55.0%
	TCTGATGATCAGGCCTGCCT+AGG	+	chr8.4:62062222-62062241	MS.gene87845:intron	55.0%
	TGACGGTCTCACCTTGTCTG+AGG	+	chr8.4:62060973-62060992	MS.gene87845:CDS	55.0%
	TTATCCGACGTTCCCGTCGA+AGG	+	chr8.4:62062755-62062774	MS.gene87845:intron	55.0%
!	ACGGTCAGGAAGCATGCTAG+AGG	-	chr8.4:62061959-62061978	None:intergenic	55.0%
!	ATGCGTGGCATCTAGAACCG+GGG	+	chr8.4:62062990-62063009	MS.gene87845:CDS	55.0%
!	GCAGCAGCATTGAGAGGAGA+TGG	-	chr8.4:62061733-62061752	None:intergenic	55.0%
!	GCATCTAGAACCGGGGTTCA+AGG	+	chr8.4:62062997-62063016	MS.gene87845:CDS	55.0%
!	GTCACAGGTGTGGACTGTTG+GGG	+	chr8.4:62061554-62061573	MS.gene87845:CDS	55.0%
!!	TGGTGCTACAGGCGAAGATC+AGG	+	chr8.4:62063177-62063196	MS.gene87845:intron	55.0%
	ATGTCTCGCGCTTGGAAGCC+AGG	+	chr8.4:62061112-62061131	MS.gene87845:CDS	60.0%
	CCAAGCACCAATGGACGGTC+AGG	-	chr8.4:62061973-62061992	None:intergenic	60.0%
	CTCCACGTGCGGTGGAAGAA+AGG	+	chr8.4:62062810-62062829	MS.gene87845:intron	60.0%
	GAGGTCGTAGATCAGCCGCT+TGG	+	chr8.4:62062579-62062598	MS.gene87845:intron	60.0%
	GCTCGCGCTAACACTGCTGA+AGG	+	chr8.4:62061760-62061779	MS.gene87845:CDS	60.0%
	GCTTGGAATTGCCGGGGTGT+AGG	+	chr8.4:62062260-62062279	MS.gene87845:intron	60.0%
	GTGCGGAGGACATGTGCGAA+TGG	+	chr8.4:62063083-62063102	MS.gene87845:intron	60.0%
	TCTTGGTACTCCACGTGCGG+TGG	+	chr8.4:62062802-62062821	MS.gene87845:intron	60.0%
!	CCTGACCGTCCATTGGTGCT+TGG	+	chr8.4:62061970-62061989	MS.gene87845:CDS	60.0%
!	GCAGGAGCAGCAGCATTGAG+AGG	-	chr8.4:62061739-62061758	None:intergenic	60.0%
!	GTAGACCGCACCGGTAGATC+CGG	+	chr8.4:62062422-62062441	MS.gene87845:intron	60.0%
!	TAGACCGCACCGGTAGATCC+GGG	+	chr8.4:62062423-62062442	MS.gene87845:intron	60.0%
!!	CCGGATTGAAGTGCCGGACT+AGG	-	chr8.4:62062316-62062335	None:intergenic	60.0%
!!	GAGACACGGCTTGGTGCTAC+AGG	+	chr8.4:62063166-62063185	MS.gene87845:intron	60.0%
	AACCCCCGGATCTACCGGTG+CGG	-	chr8.4:62062430-62062449	None:intergenic	65.0%
	CACTCGGGCTACCTACACCC+CGG	-	chr8.4:62062274-62062293	None:intergenic	65.0%
	CTGCTGCCCTCTTCCTCCAC+AGG	+	chr8.4:62061925-62061944	MS.gene87845:CDS	65.0%
	GCAGTGTTAGCGCGAGCAGC+AGG	-	chr8.4:62061757-62061776	None:intergenic	65.0%
	GCGAGCGTATGTCTCGCGCT+TGG	+	chr8.4:62061104-62061123	MS.gene87845:CDS	65.0%
!	AGACCGCACCGGTAGATCCG+GGG	+	chr8.4:62062424-62062443	MS.gene87845:intron	65.0%
!	GACCGCACCGGTAGATCCGG+GGG	+	chr8.4:62062425-62062444	MS.gene87845:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	62060950	62063212	62060950	ID=MS.gene87845
chr8.4	mRNA	62060950	62063212	62060950	ID=MS.gene87845.t1;Parent=MS.gene87845
chr8.4	exon	62060950	62062146	62060950	ID=MS.gene87845.t1.exon1;Parent=MS.gene87845.t1
chr8.4	CDS	62060950	62062146	62060950	ID=cds.MS.gene87845.t1;Parent=MS.gene87845.t1
chr8.4	exon	62062893	62063095	62062893	ID=MS.gene87845.t1.exon2;Parent=MS.gene87845.t1
chr8.4	CDS	62062893	62063095	62062893	ID=cds.MS.gene87845.t1;Parent=MS.gene87845.t1
chr8.4	exon	62063188	62063212	62063188	ID=MS.gene87845.t1.exon3;Parent=MS.gene87845.t1
chr8.4	CDS	62063188	62063212	62063188	ID=cds.MS.gene87845.t1;Parent=MS.gene87845.t1

Gene Sequence

>MS.gene87845

ATGGATCCCACCAACCTGAATCTTGACGGTCTCACCTTGTCTGAGGAAGGTCTCGTCCTTAACCTTGAGTCTGAACCAGAAAATGCTGCTGTCCTTGAACACTGTCTCATAGGTCGAGTCCTGGCTGATCGCGAGATTCAATTTGCCTATTTCAGCGAGCGTATGTCTCGCGCTTGGAAGCCAGGCAAAAAGGTCACGATCACCAAATCTGTTGCTGATCGCTACTTGTTCCAATTTCACCATAAGGTTGATGCTGCCCGTGTTCTTGATGAAGGGCCATGGCTTTATGATAATTTTCATATTGTTATGGACCGTATTTCCCCTGGTGTCGTCCCAAGTTTTGTCCCGTTGAATCATATCGAGTTCTGGATTCAGGTCCATGGTTTACCATTTGGTTTCATTCAACCTAAGGTAGGTCAAGGTATAGGGAGTTTTCTAGGTACCCTCAAAGCTTATGATGGCCGCAACACCATTCATAGCTCTTACATGAGACTTAAGGTTGCTATTGACGTCACTGTTCCATTGAAACAAGAGTGGCGTGTTCGTGCTACTAATGGCGAATTTGTTACTGTAACCTTTAAGTATGAAAAACTTGGTATTTTTTGTCACAGGTGTGGACTGTTGGGGCATACTGACAAGGTGTGTCCAGACTTGTTCGAGCTTGAATCTGATGATGGAGTCCGTAGATGGGGACCGTATTTAAAGCCTTCATCTCAAAGAATTGGAACAGCTGCTACTAACAGGTGGTTACAGGACCCTATTCCTGTCTCTATGTCGCAGCCATCTCCTCTCAATGCTGCTGCTCCTGCTGCTCGCGCTAACACTGCTGAAGGCAATGGAAACAATGCTAATTTTCATGACCGCATGATGGCTTTTCAGTCTCAACTCACTGCTATGAAACAAGATGTTCTTGCTGCCCAAAATGCTGTATTGGCTAAAAAAGGTAATGGTGTGAATTCTGCTTGCAAAGTGCATCTGCTGCCCTCTTCCTCCACAGGTACAACAGCCTCTAGCATGCTTCCTGACCGTCCATTGGTGCTTGGATTACCTGCTGTTCCGGTTTCAACCGAATCCGATGCTGAGATACAAGAGGAAAGTGGTTCGGATTTGAAGAAAAGGAAGCGTATGTTGGACCGTCAGAATGAGTTATCATTTGCTGCTGATGGGATTATGGGAAATGTTGTAATAGGATCCAATGTTAGTCATGGGGGGGATGATGATATGTATGTAACTGCTGGTCCTATAGATGATGAACAAGTTGTAATGGCAGGTTCTGATGATCAGGCCTGCCTAGGTGAATGAGTCTAGTAGCTTGGAATTGCCGGGGTGTAGGTAGCCCGAGTGCAATTCCCGACCTTAAATACCTAGTCCGGCACTTCAATCCGGATCTTTTATTCCTAAGTGAGACACTAGCTCACCGAAATAAAATTGAAGAACTCCGTTTTTTGCTTGGTTACGATTCTTGTCTTCATGTAGACCGCACCGGTAGATCCGGGGGTTTAGCTTTATTTTGGAAAGACTCTTTACATTGTCAGCTTGTAAATTTTTCTAACAACCATATTACTGTTGAAATTGTTAACAATACTCTTGGTAATTGGCGTGTTACCGGCTATTATGGCTATCCTAATGGAGGTCGTAGATCAGCCGCTTGGAATTTTCTTCGACAACTTTCTTCTCAATTTTCAGGTCCATGGTGCATCCTAGGAGATTTTAATGATATCTTAGATGCAAGTGAGAAAAGGGGACGAAATTTAAGACCCCAATGGCTTATCAATGGCTTCCGTCAAGCGGTTCTTGATTCAGGTTTATCCGACGTTCCCGTCGAAGGTTATCCGTACACTTGGTTTAAAAGTCTTGGTACTCCACGTGCGGTGGAAGAAAGGTTAGATCGTGCTCTTGCAAATGATTTATGGTTTAATATTTTCCCGCATGCTACTGTTGAGACTCTTGTGGCACCTGCTTCCGATCATTACCCTATTCTTGTCAATGTTGCACCTACACCTCGGTTACAGATTCCTAAACGTCATTTTCGCTACGAAAATGCGTGGCATCTAGAACCGGGGTTCAAGGACTTTGTTACTAATTCTTGGCAGGTTTATTCTACAAGTACTCTTCTTCCCAAATTATCTTCATGTGCGGAGGACATGTGCGAATGGAAGAAAACTCATTGCCACAAATTAAAAAAAGACATTGAAGATTGTCGCAAACAATTACAAGAGACACGGCTTGGTGCTACAGGCGAAGATCAGGTTCGAATGTTTGA

Protein sequence

>MS.gene87845.t1

MDPTNLNLDGLTLSEEGLVLNLESEPENAAVLEHCLIGRVLADREIQFAYFSERMSRAWKPGKKVTITKSVADRYLFQFHHKVDAARVLDEGPWLYDNFHIVMDRISPGVVPSFVPLNHIEFWIQVHGLPFGFIQPKVGQGIGSFLGTLKAYDGRNTIHSSYMRLKVAIDVTVPLKQEWRVRATNGEFVTVTFKYEKLGIFCHRCGLLGHTDKVCPDLFELESDDGVRRWGPYLKPSSQRIGTAATNRWLQDPIPVSMSQPSPLNAAAPAARANTAEGNGNNANFHDRMMAFQSQLTAMKQDVLAAQNAVLAKKGNGVNSACKVHLLPSSSTGTTASSMLPDRPLVLGLPAVPVSTESDAEIQEESGSDLKKRKRMLDRQNELSFAADGIMGNVVIGSNTLVAPASDHYPILVNVAPTPRLQIPKRHFRYENAWHLEPGFKDFVTNSWQVYSTSTLLPKLSSCAEDMRRSGSNV