AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72520.t1 MTR_3g053920 44.939 247 63 5 1 243 103 280 1.96e-50 170
MS.gene72520.t1 MTR_8g463110 57.600 125 44 3 187 309 1 118 6.42e-26 109
MS.gene72520.t1 MTR_4g103530 69.388 49 15 0 1 49 49 97 2.93e-17 79.3
MS.gene72520.t1 MTR_6g033215 36.364 99 61 1 1 97 196 294 6.30e-17 80.5
MS.gene72520.t1 MTR_0010s0370 43.478 69 35 2 10 75 110 177 1.58e-11 63.2
MS.gene72520.t1 MTR_0010s0350 43.478 69 35 2 10 75 110 177 1.58e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72520.t1 AT5G36228 30.172 116 76 2 15 125 118 233 3.67e-12 67.0
MS.gene72520.t1 AT2G17920 28.283 99 69 1 18 114 121 219 2.04e-11 64.7

Find 98 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGCTGCTGATGGGGTT+GGG 0.160055 4.1:+63459385 MS.gene72520:CDS
CTCTGAATCATTTGGATATC+TGG 0.168086 4.1:+63458560 MS.gene72520:CDS
CGGACGATGGCGTACGTAAT+TGG 0.201201 4.1:+63458881 MS.gene72520:CDS
GATATCTGGGTGCAGGTATA+TGG 0.248137 4.1:+63458574 MS.gene72520:CDS
TCCTTTGCTGCTGATGGGGT+TGG 0.252686 4.1:+63459384 MS.gene72520:CDS
CTTTGCTGCTGATGGGGTTG+GGG 0.267138 4.1:+63459386 MS.gene72520:CDS
GGACGATGGCGTACGTAATT+GGG 0.303838 4.1:+63458882 MS.gene72520:CDS
TTTACTGCAGGGTCGTTCTA+TGG 0.328442 4.1:+63459224 MS.gene72520:CDS
CACTGTTCCTCTGAATCATT+TGG 0.330851 4.1:+63458552 MS.gene72520:CDS
TGTCACTGCACCGCTGAAAA+AGG 0.338620 4.1:+63458723 MS.gene72520:CDS
AAACCTGTTACGACTCGTAT+AGG 0.344071 4.1:+63458916 MS.gene72520:CDS
CAGGGTCGTTCTATGGTACT+TGG 0.351644 4.1:+63459231 MS.gene72520:CDS
GAGTCCTCCTTTGCTGCTGA+TGG 0.351810 4.1:+63459378 MS.gene72520:CDS
CAAGGCATTGGCAGCTTTCT+CGG 0.355349 4.1:+63458631 MS.gene72520:CDS
TCTGCGCACGCAACAAGCTT+TGG 0.361489 4.1:-63460352 None:intergenic
CTTTGATGCGGCACAACATC+AGG 0.362158 4.1:-63458976 None:intergenic
GACCCTATACGAGTCGTAAC+AGG 0.367774 4.1:-63458919 None:intergenic
CAGGTATATGGATTGCCCTT+TGG 0.372079 4.1:+63458586 MS.gene72520:CDS
TGCTACAAACAGATGGCTTC+AGG 0.376139 4.1:+63458945 MS.gene72520:CDS
AATGGTGTTGCGACCATCAT+AGG 0.380913 4.1:-63458665 None:intergenic
AGTCCTCCTTTGCTGCTGAT+GGG 0.390042 4.1:+63459379 MS.gene72520:CDS
TGCTATTCATGCTATTAAAC+AGG 0.391667 4.1:+63459095 MS.gene72520:CDS
AACCTGTTACGACTCGTATA+GGG 0.403226 4.1:+63458917 MS.gene72520:CDS
TTTGCTGCTGATGGGGTTGG+GGG 0.419832 4.1:+63459387 MS.gene72520:CDS
GTGTCCCGACTTGTTTGAAT+TGG 0.427535 4.1:+63458855 MS.gene72520:CDS
TGTTGCGTGCGCAGAAGATA+TGG 0.433763 4.1:+63460360 MS.gene72520:CDS
CTCGGCCTCAAATTTACTGC+AGG 0.434485 4.1:+63459212 MS.gene72520:CDS
TCTGAATCATTTGGATATCT+GGG 0.440164 4.1:+63458561 MS.gene72520:CDS
GTTGTTCTTCTTGAGGATGC+TGG 0.443839 4.1:+63459309 MS.gene72520:CDS
CCTATATGCGCCTCAAAGTC+CGG 0.446535 4.1:+63458695 MS.gene72520:CDS
CTTTGGTTTCATTCAATCAA+AGG 0.459068 4.1:+63458603 MS.gene72520:CDS
CCGGACTTTGAGGCGCATAT+AGG 0.464440 4.1:-63458695 None:intergenic
GGTGCGGCCAACAGAAGAAC+TGG 0.478813 4.1:-63459010 None:intergenic
GCAAAAGTGAAAGGAGCAGC+AGG 0.480319 4.1:-63459258 None:intergenic
GTCGTAACAGGTTTGAGATC+AGG 0.483266 4.1:-63458907 None:intergenic
CGACTTGTTTGAATTGGACT+CGG 0.483849 4.1:+63458861 MS.gene72520:CDS
TTTGAATTGGACTCGGACGA+TGG 0.484571 4.1:+63458868 MS.gene72520:CDS
CAAGCTAAACATGTCACAAA+TGG 0.496742 4.1:+63459144 MS.gene72520:CDS
ATAGGATCCAATGTTAGTGC+TGG 0.499466 4.1:+63459420 MS.gene72520:CDS
CGACAACGTTGTTCTTCTTG+AGG 0.503735 4.1:+63459302 MS.gene72520:CDS
TTCCAAGCCAGTTCTTCTGT+TGG 0.504058 4.1:+63459003 MS.gene72520:CDS
ATGCTTCCATCTCCATCTGC+AGG 0.507192 4.1:+63459186 MS.gene72520:CDS
GGTTTCATTCAATCAAAGGT+TGG 0.507457 4.1:+63458607 MS.gene72520:CDS
TGTTGCGACCATCATAGGTT+AGG 0.510688 4.1:-63458660 None:intergenic
GTCATGGAACGCATTGCTCC+TGG 0.512312 4.1:+63458517 None:intergenic
GTCCTCCTTTGCTGCTGATG+GGG 0.512438 4.1:+63459380 MS.gene72520:CDS
TCAAAGGTTGGTCAAGGCAT+TGG 0.512470 4.1:+63458619 MS.gene72520:CDS
TTTGATTGAATGAAACCAAA+GGG 0.513269 4.1:-63458601 None:intergenic
ATCTCCATCTGCAGGAGTCT+CGG 0.517427 4.1:+63459194 MS.gene72520:CDS
GCGCAGAAGATATGGATGCT+TGG 0.517659 4.1:+63460368 MS.gene72520:CDS
TGACATCAATCCGGACTTTG+AGG 0.517759 4.1:-63458705 None:intergenic
CCCAACCCCATCAGCAGCAA+AGG 0.519433 4.1:-63459385 None:intergenic
ACGACCCTGCAGTAAATTTG+AGG 0.521272 4.1:-63459217 None:intergenic
GTGTGGCATTCTCAGACATT+CGG 0.523325 4.1:+63458825 MS.gene72520:CDS
GGAAAGTTCGTGCTAGCAAT+GGG 0.524395 4.1:+63458749 MS.gene72520:CDS
TAGGATCCAATGTTAGTGCT+GGG 0.529611 4.1:+63459421 MS.gene72520:CDS
TCATTTGGATATCTGGGTGC+AGG 0.530536 4.1:+63458567 MS.gene72520:CDS
GAGACTCCTGCAGATGGAGA+TGG 0.535925 4.1:-63459192 None:intergenic
TGGAAAGTTCGTGCTAGCAA+TGG 0.538781 4.1:+63458748 MS.gene72520:CDS
ATCCAATGTTAGTGCTGGGG+GGG 0.539930 4.1:+63459425 MS.gene72520:CDS
ATTCAATCAAAGGTTGGTCA+AGG 0.545909 4.1:+63458613 MS.gene72520:CDS
GAGTCCAATTCAAACAAGTC+GGG 0.547426 4.1:-63458859 None:intergenic
TCAAAACAGAATTGTCAGCA+TGG 0.555985 4.1:-63459032 None:intergenic
ATGCGGCACAACATCAGGGA+TGG 0.556865 4.1:-63458971 None:intergenic
TTTGTACCAGCAAAAGTGAA+AGG 0.560500 4.1:-63459267 None:intergenic
CAGAATTGTCAGCATGGGTG+CGG 0.561489 4.1:-63459026 None:intergenic
GGTACTCTCCTAACCTATGA+TGG 0.562210 4.1:+63458652 MS.gene72520:CDS
TGTATGATCGCCCTTCAGAA+TGG 0.570414 4.1:+63459354 MS.gene72520:CDS
TGATTCAGAGGAACAGTGAT+CGG 0.572579 4.1:-63458547 None:intergenic
GGCCAACAGAAGAACTGGCT+TGG 0.572612 4.1:-63459005 None:intergenic
CAAAACAGAATTGTCAGCAT+GGG 0.573225 4.1:-63459031 None:intergenic
ACAGTGATCGGAACCACACC+AGG 0.573710 4.1:-63458535 None:intergenic
GCGGTGCAGTGACATCAATC+CGG 0.575241 4.1:-63458714 None:intergenic
AGGAGGACTCTCCATTCTGA+AGG 0.582009 4.1:-63459365 None:intergenic
GACGATGGCGTACGTAATTG+GGG 0.582951 4.1:+63458883 MS.gene72520:CDS
GAGGCCGAGACTCCTGCAGA+TGG 0.584099 4.1:-63459198 None:intergenic
GCAGATGGAGATGGAAGCAT+CGG 0.585916 4.1:-63459183 None:intergenic
CAGTGTCTACAGCAAGCATA+CGG 0.590441 4.1:-63459074 None:intergenic
TGCGGCACAACATCAGGGAT+GGG 0.590978 4.1:-63458970 None:intergenic
GGATCCAATGTTAGTGCTGG+GGG 0.592557 4.1:+63459423 MS.gene72520:CDS
AACCCCATCAGCAGCAAAGG+AGG 0.593836 4.1:-63459382 None:intergenic
GCTTGGAAGTTACTTTGATG+CGG 0.594151 4.1:-63458988 None:intergenic
CATTCTCAGACATTCGGACA+AGG 0.594459 4.1:+63458831 MS.gene72520:CDS
CAGATATCCAAATGATTCAG+AGG 0.599954 4.1:-63458559 None:intergenic
AAACATGTCACAAATGGTGC+TGG 0.605638 4.1:+63459150 MS.gene72520:CDS
GGATGTCCTCGCTGCTCAGA+CGG 0.607559 4.1:+63459116 MS.gene72520:CDS
CTTTGATTGAATGAAACCAA+AGG 0.608719 4.1:-63458602 None:intergenic
CGAGTCCAATTCAAACAAGT+CGG 0.610252 4.1:-63458860 None:intergenic
GGTCGGCTGCTACAAACAGA+TGG 0.622085 4.1:+63458938 MS.gene72520:CDS
GCGCATATAGGAGCTATGAA+TGG 0.625349 4.1:-63458683 None:intergenic
TTTGATGCGGCACAACATCA+GGG 0.625918 4.1:-63458975 None:intergenic
GGAGGACTCTCCATTCTGAA+GGG 0.629990 4.1:-63459364 None:intergenic
TCGGCCTCAAATTTACTGCA+GGG 0.638651 4.1:+63459213 MS.gene72520:CDS
GGAACGCATTGCTCCTGGTG+TGG 0.640570 4.1:+63458522 MS.gene72520:CDS
AGGATCCAATGTTAGTGCTG+GGG 0.645296 4.1:+63459422 MS.gene72520:CDS
GATCCAATGTTAGTGCTGGG+GGG 0.647286 4.1:+63459424 MS.gene72520:CDS
GCAGAGCCGTCTGAGCAGCG+AGG 0.708384 4.1:-63459122 None:intergenic
TGTTACGACTCGTATAGGGT+CGG 0.713178 4.1:+63458921 MS.gene72520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTATTAGAAAAATCAATA+AGG - chr4.1:63459772-63459791 None:intergenic 15.0%
!! TTTGAAAAAAATAGTAACAT+AGG - chr4.1:63458776-63458795 None:intergenic 15.0%
!!! GAAGTTCTTCAATTTTATTT+CGG - chr4.1:63459655-63459674 None:intergenic 20.0%
!!! GGAGAACATTTTAATAAAAT+AGG - chr4.1:63460211-63460230 None:intergenic 20.0%
!!! TTTTTCAAATATGAGAAACT+GGG + chr4.1:63458787-63458806 MS.gene72520:CDS 20.0%
!!! TTTTTTCAAATATGAGAAAC+TGG + chr4.1:63458786-63458805 MS.gene72520:CDS 20.0%
! GTATGATGAAAAAGATGTAA+TGG + chr4.1:63459479-63459498 MS.gene72520:intron 25.0%
! TTTGATTGAATGAAACCAAA+GGG - chr4.1:63458604-63458623 None:intergenic 25.0%
!! TTTTTCATCATACAAAGGAT+TGG - chr4.1:63459472-63459491 None:intergenic 25.0%
!!! ACATCTTTTTCATCATACAA+AGG - chr4.1:63459477-63459496 None:intergenic 25.0%
CTTTGATTGAATGAAACCAA+AGG - chr4.1:63458605-63458624 None:intergenic 30.0%
TCACTTAGGAATAAAAGATC+CGG - chr4.1:63459620-63459639 None:intergenic 30.0%
TCTGAATCATTTGGATATCT+GGG + chr4.1:63458561-63458580 MS.gene72520:CDS 30.0%
TGCTATTCATGCTATTAAAC+AGG + chr4.1:63459095-63459114 MS.gene72520:CDS 30.0%
TTCACATGGTTTAAAAGTCT+CGG + chr4.1:63460070-63460089 MS.gene72520:intron 30.0%
TTTAAATCGGGAATTGTACT+CGG - chr4.1:63459575-63459594 None:intergenic 30.0%
! AGACTTTTAAACCATGTGAA+AGG - chr4.1:63460070-63460089 None:intergenic 30.0%
! CTTTGGTTTCATTCAATCAA+AGG + chr4.1:63458603-63458622 MS.gene72520:CDS 30.0%
AAACCACATAGTGTTAGCAA+GGG - chr4.1:63460131-63460150 None:intergenic 35.0%
AAATCACCAAAAATGCACCA+AGG - chr4.1:63459926-63459945 None:intergenic 35.0%
AATCACCAAAAATGCACCAA+GGG - chr4.1:63459925-63459944 None:intergenic 35.0%
AGAGTTTCAACTGTAGCATT+CGG - chr4.1:63460163-63460182 None:intergenic 35.0%
AGGATCTTGTTACTAACTCA+TGG + chr4.1:63460302-63460321 MS.gene72520:intron 35.0%
ATGACGAAGAAAATTCCAAG+CGG - chr4.1:63459882-63459901 None:intergenic 35.0%
ATTAGGATAGCCATAATAGC+CGG - chr4.1:63459843-63459862 None:intergenic 35.0%
CAAAACAGAATTGTCAGCAT+GGG - chr4.1:63459034-63459053 None:intergenic 35.0%
CAAGCTAAACATGTCACAAA+TGG + chr4.1:63459144-63459163 MS.gene72520:CDS 35.0%
CAGATATCCAAATGATTCAG+AGG - chr4.1:63458562-63458581 None:intergenic 35.0%
CATCTCTCTAATCAATTTGC+AGG + chr4.1:63459899-63459918 MS.gene72520:intron 35.0%
CTCTGAATCATTTGGATATC+TGG + chr4.1:63458560-63458579 MS.gene72520:CDS 35.0%
GATGAAAAAGATGTAATGGC+AGG + chr4.1:63459483-63459502 MS.gene72520:intron 35.0%
GCTATTAGACTCATTTACCT+TGG - chr4.1:63459527-63459546 None:intergenic 35.0%
GGTTTCATTCAATCAAAGGT+TGG + chr4.1:63458607-63458626 MS.gene72520:CDS 35.0%
TAAAATGTTCTCCTTCACCT+CGG + chr4.1:63460218-63460237 MS.gene72520:intron 35.0%
TAAACCACATAGTGTTAGCA+AGG - chr4.1:63460132-63460151 None:intergenic 35.0%
TACCTGAACATCTGATAAAC+CGG - chr4.1:63460038-63460057 None:intergenic 35.0%
TATTATGGCTATCCTAATGG+AGG + chr4.1:63459845-63459864 MS.gene72520:intron 35.0%
TCAAAACAGAATTGTCAGCA+TGG - chr4.1:63459035-63459054 None:intergenic 35.0%
TCATATCACTGTTGAGATTG+TGG + chr4.1:63459790-63459809 MS.gene72520:intron 35.0%
TGGATTCTGATCATGGTAAT+TGG + chr4.1:63459810-63459829 MS.gene72520:intron 35.0%
TTAAATCGGGAATTGTACTC+GGG - chr4.1:63459574-63459593 None:intergenic 35.0%
TTATCAGATGTTCAGGTAGA+AGG + chr4.1:63460040-63460059 MS.gene72520:intron 35.0%
TTTGTACCAGCAAAAGTGAA+AGG - chr4.1:63459270-63459289 None:intergenic 35.0%
! ATTCAATCAAAGGTTGGTCA+AGG + chr4.1:63458613-63458632 MS.gene72520:CDS 35.0%
!!! AACTGGGTGTTTTTTGTTAC+AGG + chr4.1:63458803-63458822 MS.gene72520:CDS 35.0%
!!! GAAGAACTTCGTTTTTTGCT+TGG + chr4.1:63459665-63459684 MS.gene72520:intron 35.0%
AAACATGTCACAAATGGTGC+TGG + chr4.1:63459150-63459169 MS.gene72520:CDS 40.0%
AAACCTGTTACGACTCGTAT+AGG + chr4.1:63458916-63458935 MS.gene72520:CDS 40.0%
AACCTGTTACGACTCGTATA+GGG + chr4.1:63458917-63458936 MS.gene72520:CDS 40.0%
ATAGGATCCAATGTTAGTGC+TGG + chr4.1:63459420-63459439 MS.gene72520:CDS 40.0%
CACTGTTCCTCTGAATCATT+TGG + chr4.1:63458552-63458571 MS.gene72520:CDS 40.0%
CATGGTAATTGGAGAATGAC+CGG + chr4.1:63459821-63459840 MS.gene72520:intron 40.0%
CGAGTCCAATTCAAACAAGT+CGG - chr4.1:63458863-63458882 None:intergenic 40.0%
GAGATTGTGGATTCTGATCA+TGG + chr4.1:63459803-63459822 MS.gene72520:intron 40.0%
GAGTCCAATTCAAACAAGTC+GGG - chr4.1:63458862-63458881 None:intergenic 40.0%
GGCTATTATGGCTATCCTAA+TGG + chr4.1:63459842-63459861 MS.gene72520:intron 40.0%
GGTAAATGAGTCTAATAGCG+TGG + chr4.1:63459528-63459547 MS.gene72520:intron 40.0%
GTAACAAGATCCTTAAACCC+CGG - chr4.1:63460295-63460314 None:intergenic 40.0%
GTCTAATAGCGTGGAATTGT+CGG + chr4.1:63459537-63459556 MS.gene72520:intron 40.0%
GTTGGGGGAATTGATGTAAT+AGG + chr4.1:63459402-63459421 MS.gene72520:CDS 40.0%
TAGAAGGCTATCCTTTCACA+TGG + chr4.1:63460056-63460075 MS.gene72520:intron 40.0%
TAGGATCCAATGTTAGTGCT+GGG + chr4.1:63459421-63459440 MS.gene72520:CDS 40.0%
TCTAATAGCGTGGAATTGTC+GGG + chr4.1:63459538-63459557 MS.gene72520:intron 40.0%
TCTTGATGCTAGTGAGAAGA+GGG + chr4.1:63459955-63459974 MS.gene72520:intron 40.0%
TCTTGTTACTAACTCATGGC+AGG + chr4.1:63460306-63460325 MS.gene72520:intron 40.0%
TGATTCAGAGGAACAGTGAT+CGG - chr4.1:63458550-63458569 None:intergenic 40.0%
TTAGACTCATTTACCTTGGC+AGG - chr4.1:63459523-63459542 None:intergenic 40.0%
TTCTTGATGCTAGTGAGAAG+AGG + chr4.1:63459954-63459973 MS.gene72520:intron 40.0%
! ATTTTCGCTACGAAAATGCG+TGG + chr4.1:63460260-63460279 MS.gene72520:intron 40.0%
! CCGATTTAAAGTACCTAGTC+CGG + chr4.1:63459585-63459604 MS.gene72520:intron 40.0%
! CCGGACTAGGTACTTTAAAT+CGG - chr4.1:63459588-63459607 None:intergenic 40.0%
! CGACTTGTTTGAATTGGACT+CGG + chr4.1:63458861-63458880 MS.gene72520:CDS 40.0%
! CGGACTAGGTACTTTAAATC+GGG - chr4.1:63459587-63459606 None:intergenic 40.0%
! GCTTGGAAGTTACTTTGATG+CGG - chr4.1:63458991-63459010 None:intergenic 40.0%
!!! ACTTTCCACTCCTTTTTCAG+CGG - chr4.1:63458736-63458755 None:intergenic 40.0%
!!! GGTGTTTTTTGTTACAGGTG+TGG + chr4.1:63458808-63458827 MS.gene72520:CDS 40.0%
AAAGATCCGGATTGAAGTGC+CGG - chr4.1:63459607-63459626 None:intergenic 45.0%
AAATGCGTGGCAGTTAGAAC+CGG + chr4.1:63460273-63460292 MS.gene72520:intron 45.0%
AAATTGCGCCTATGTTGCTG+AGG - chr4.1:63460244-63460263 None:intergenic 45.0%
ACATAGTGTTAGCAAGGGCA+CGG - chr4.1:63460126-63460145 None:intergenic 45.0%
ACGACCCTGCAGTAAATTTG+AGG - chr4.1:63459220-63459239 None:intergenic 45.0%
AGGATCCAATGTTAGTGCTG+GGG + chr4.1:63459422-63459441 MS.gene72520:CDS 45.0%
ATAGGATAGTGATCAGACGC+AGG - chr4.1:63460193-63460212 None:intergenic 45.0%
CAGTGTCTACAGCAAGCATA+CGG - chr4.1:63459077-63459096 None:intergenic 45.0%
CATCAATGGCTTTCGTGAAG+CGG + chr4.1:63460003-63460022 MS.gene72520:intron 45.0%
CATTCTCAGACATTCGGACA+AGG + chr4.1:63458831-63458850 MS.gene72520:CDS 45.0%
CGACAACGTTGTTCTTCTTG+AGG + chr4.1:63459302-63459321 MS.gene72520:CDS 45.0%
CTAATAGCGTGGAATTGTCG+GGG + chr4.1:63459539-63459558 MS.gene72520:intron 45.0%
CTAATCAATTTGCAGGCCCT+TGG + chr4.1:63459906-63459925 MS.gene72520:intron 45.0%
CTCCGGTTTATCAGATGTTC+AGG + chr4.1:63460033-63460052 MS.gene72520:intron 45.0%
CTTGATGCTAGTGAGAAGAG+GGG + chr4.1:63459956-63459975 MS.gene72520:intron 45.0%
GATATCTGGGTGCAGGTATA+TGG + chr4.1:63458574-63458593 MS.gene72520:CDS 45.0%
GCGCATATAGGAGCTATGAA+TGG - chr4.1:63458686-63458705 None:intergenic 45.0%
GGAAAGTTCGTGCTAGCAAT+GGG + chr4.1:63458749-63458768 MS.gene72520:CDS 45.0%
GGTACTCTCCTAACCTATGA+TGG + chr4.1:63458652-63458671 MS.gene72520:CDS 45.0%
GTGTGGCATTCTCAGACATT+CGG + chr4.1:63458825-63458844 MS.gene72520:CDS 45.0%
GTTGTTCTTCTTGAGGATGC+TGG + chr4.1:63459309-63459328 MS.gene72520:CDS 45.0%
TCATTTGGATATCTGGGTGC+AGG + chr4.1:63458567-63458586 MS.gene72520:CDS 45.0%
TCGGCCTCAAATTTACTGCA+GGG + chr4.1:63459213-63459232 MS.gene72520:CDS 45.0%
TGACATCAATCCGGACTTTG+AGG - chr4.1:63458708-63458727 None:intergenic 45.0%
TGCTACAAACAGATGGCTTC+AGG + chr4.1:63458945-63458964 MS.gene72520:CDS 45.0%
TGGAAAGTTCGTGCTAGCAA+TGG + chr4.1:63458748-63458767 MS.gene72520:CDS 45.0%
TGGAGAATGACCGGCTATTA+TGG + chr4.1:63459830-63459849 MS.gene72520:intron 45.0%
TGTATGATCGCCCTTCAGAA+TGG + chr4.1:63459354-63459373 MS.gene72520:CDS 45.0%
TGTTACGACTCGTATAGGGT+CGG + chr4.1:63458921-63458940 MS.gene72520:CDS 45.0%
TGTTGCGACCATCATAGGTT+AGG - chr4.1:63458663-63458682 None:intergenic 45.0%
TTCCAAGCCAGTTCTTCTGT+TGG + chr4.1:63459003-63459022 MS.gene72520:CDS 45.0%
TTGATGCTAGTGAGAAGAGG+GGG + chr4.1:63459957-63459976 MS.gene72520:intron 45.0%
TTTACTGCAGGGTCGTTCTA+TGG + chr4.1:63459224-63459243 MS.gene72520:CDS 45.0%
TTTGAATTGGACTCGGACGA+TGG + chr4.1:63458868-63458887 MS.gene72520:CDS 45.0%
TTTGATGCGGCACAACATCA+GGG - chr4.1:63458978-63458997 None:intergenic 45.0%
! CAGGTATATGGATTGCCCTT+TGG + chr4.1:63458586-63458605 MS.gene72520:CDS 45.0%
! GTCGTAACAGGTTTGAGATC+AGG - chr4.1:63458910-63458929 None:intergenic 45.0%
! GTGTCCCGACTTGTTTGAAT+TGG + chr4.1:63458855-63458874 MS.gene72520:CDS 45.0%
! TCAAAGGTTGGTCAAGGCAT+TGG + chr4.1:63458619-63458638 MS.gene72520:CDS 45.0%
!! AATGGTGTTGCGACCATCAT+AGG - chr4.1:63458668-63458687 None:intergenic 45.0%
AAGCCATTGATGAGCCACTG+TGG - chr4.1:63459995-63460014 None:intergenic 50.0%
AATGCGTGGCAGTTAGAACC+GGG + chr4.1:63460274-63460293 MS.gene72520:intron 50.0%
AATGGCAGGACCTGATGATC+AGG + chr4.1:63459497-63459516 MS.gene72520:intron 50.0%
AGACCACAGTGGCTCATCAA+TGG + chr4.1:63459989-63460008 MS.gene72520:intron 50.0%
AGGAGGACTCTCCATTCTGA+AGG - chr4.1:63459368-63459387 None:intergenic 50.0%
AGTCCTCCTTTGCTGCTGAT+GGG + chr4.1:63459379-63459398 MS.gene72520:CDS 50.0%
ATCTCCATCTGCAGGAGTCT+CGG + chr4.1:63459194-63459213 MS.gene72520:CDS 50.0%
ATGCTTCCATCTCCATCTGC+AGG + chr4.1:63459186-63459205 MS.gene72520:CDS 50.0%
CAGAATTGTCAGCATGGGTG+CGG - chr4.1:63459029-63459048 None:intergenic 50.0%
CAGGGTCGTTCTATGGTACT+TGG + chr4.1:63459231-63459250 MS.gene72520:CDS 50.0%
CCTATATGCGCCTCAAAGTC+CGG + chr4.1:63458695-63458714 MS.gene72520:CDS 50.0%
CTCGGCCTCAAATTTACTGC+AGG + chr4.1:63459212-63459231 MS.gene72520:CDS 50.0%
CTTTGATGCGGCACAACATC+AGG - chr4.1:63458979-63458998 None:intergenic 50.0%
GACCCTATACGAGTCGTAAC+AGG - chr4.1:63458922-63458941 None:intergenic 50.0%
GACGATGGCGTACGTAATTG+GGG + chr4.1:63458883-63458902 MS.gene72520:CDS 50.0%
GCAAAAGTGAAAGGAGCAGC+AGG - chr4.1:63459261-63459280 None:intergenic 50.0%
GCAGTTAGAACCGGGGTTTA+AGG + chr4.1:63460282-63460301 MS.gene72520:intron 50.0%
GCAGTTCTACGACCTCCATT+AGG - chr4.1:63459860-63459879 None:intergenic 50.0%
GCGCAGAAGATATGGATGCT+TGG + chr4.1:63460368-63460387 MS.gene72520:CDS 50.0%
GGACGATGGCGTACGTAATT+GGG + chr4.1:63458882-63458901 MS.gene72520:CDS 50.0%
GGAGGACTCTCCATTCTGAA+GGG - chr4.1:63459367-63459386 None:intergenic 50.0%
GGTGAGCTAGTGTCTCACTT+AGG - chr4.1:63459634-63459653 None:intergenic 50.0%
GTGCCCTTGCTAACACTATG+TGG + chr4.1:63460125-63460144 MS.gene72520:intron 50.0%
TGTCACTGCACCGCTGAAAA+AGG + chr4.1:63458723-63458742 MS.gene72520:CDS 50.0%
TGTCTTCCTATAGACCGCAC+CGG + chr4.1:63459698-63459717 MS.gene72520:intron 50.0%
TGTTGCGTGCGCAGAAGATA+TGG + chr4.1:63460360-63460379 MS.gene72520:CDS 50.0%
! ATCCAATGTTAGTGCTGGGG+GGG + chr4.1:63459425-63459444 MS.gene72520:CDS 50.0%
! GATCCAATGTTAGTGCTGGG+GGG + chr4.1:63459424-63459443 MS.gene72520:CDS 50.0%
! GCAGATGGAGATGGAAGCAT+CGG - chr4.1:63459186-63459205 None:intergenic 50.0%
! GGATCCAATGTTAGTGCTGG+GGG + chr4.1:63459423-63459442 MS.gene72520:CDS 50.0%
!! CAAGGCATTGGCAGCTTTCT+CGG + chr4.1:63458631-63458650 MS.gene72520:CDS 50.0%
!! GCTGCTCCTTTCACTTTTGC+TGG + chr4.1:63459261-63459280 MS.gene72520:CDS 50.0%
!!! GCGGGGGTCTAGCTTTATTT+TGG + chr4.1:63459726-63459745 MS.gene72520:intron 50.0%
AACCCCATCAGCAGCAAAGG+AGG - chr4.1:63459385-63459404 None:intergenic 55.0%
AACCTTTCTTCCACCGCTCG+CGG - chr4.1:63460100-63460119 None:intergenic 55.0%
ACAGTGATCGGAACCACACC+AGG - chr4.1:63458538-63458557 None:intergenic 55.0%
ATCCCCCCCAGCACTAACAT+TGG - chr4.1:63459430-63459449 None:intergenic 55.0%
ATGCGGCACAACATCAGGGA+TGG - chr4.1:63458974-63458993 None:intergenic 55.0%
ATGCGTGGCAGTTAGAACCG+GGG + chr4.1:63460275-63460294 MS.gene72520:intron 55.0%
CCTAGTCCGGCACTTCAATC+CGG + chr4.1:63459598-63459617 MS.gene72520:intron 55.0%
CCTTTGCTGCTGATGGGGTT+GGG + chr4.1:63459385-63459404 MS.gene72520:CDS 55.0%
CGGACGATGGCGTACGTAAT+TGG + chr4.1:63458881-63458900 MS.gene72520:CDS 55.0%
CTGCACCGCTGAAAAAGGAG+TGG + chr4.1:63458728-63458747 MS.gene72520:CDS 55.0%
CTTTGCTGCTGATGGGGTTG+GGG + chr4.1:63459386-63459405 MS.gene72520:CDS 55.0%
GAGACTCCTGCAGATGGAGA+TGG - chr4.1:63459195-63459214 None:intergenic 55.0%
GAGTCCTCCTTTGCTGCTGA+TGG + chr4.1:63459378-63459397 MS.gene72520:CDS 55.0%
GCGGTGCAGTGACATCAATC+CGG - chr4.1:63458717-63458736 None:intergenic 55.0%
GGCCAACAGAAGAACTGGCT+TGG - chr4.1:63459008-63459027 None:intergenic 55.0%
GGCGCAATTCTAGACCACAG+TGG + chr4.1:63459978-63459997 MS.gene72520:intron 55.0%
GGTCGGCTGCTACAAACAGA+TGG + chr4.1:63458938-63458957 MS.gene72520:CDS 55.0%
GTCCTCCTTTGCTGCTGATG+GGG + chr4.1:63459380-63459399 MS.gene72520:CDS 55.0%
TCCTTTGCTGCTGATGGGGT+TGG + chr4.1:63459384-63459403 MS.gene72520:CDS 55.0%
TCTGCGCACGCAACAAGCTT+TGG - chr4.1:63460355-63460374 None:intergenic 55.0%
TGCGGCACAACATCAGGGAT+GGG - chr4.1:63458973-63458992 None:intergenic 55.0%
TTTGCTGCTGATGGGGTTGG+GGG + chr4.1:63459387-63459406 MS.gene72520:CDS 55.0%
!! CCGGACTTTGAGGCGCATAT+AGG - chr4.1:63458698-63458717 None:intergenic 55.0%
!! CGTGAAGCGGTTATCGACTC+CGG + chr4.1:63460016-63460035 MS.gene72520:intron 55.0%
!! GCAGGCCCTTGGTGCATTTT+TGG + chr4.1:63459917-63459936 MS.gene72520:intron 55.0%
AAGTCTCGGTACACCGCGAG+CGG + chr4.1:63460084-63460103 MS.gene72520:intron 60.0%
CACCTCGGCCTCAGCAACAT+AGG + chr4.1:63460233-63460252 MS.gene72520:intron 60.0%
CCCAACCCCATCAGCAGCAA+AGG - chr4.1:63459388-63459407 None:intergenic 60.0%
CCTCTACCGGTGCGGTCTAT+AGG - chr4.1:63459707-63459726 None:intergenic 60.0%
CCTGATGATCAGGCCTGCCA+AGG + chr4.1:63459507-63459526 MS.gene72520:intron 60.0%
CCTTGGCAGGCCTGATCATC+AGG - chr4.1:63459510-63459529 None:intergenic 60.0%
GAGGTCGTAGAACTGCCGCT+TGG + chr4.1:63459864-63459883 MS.gene72520:intron 60.0%
GCGTGGAATTGTCGGGGTGT+AGG + chr4.1:63459545-63459564 MS.gene72520:intron 60.0%
GGAACGCATTGCTCCTGGTG+TGG + chr4.1:63458522-63458541 MS.gene72520:CDS 60.0%
GGATGTCCTCGCTGCTCAGA+CGG + chr4.1:63459116-63459135 MS.gene72520:CDS 60.0%
GGTGCGGCCAACAGAAGAAC+TGG - chr4.1:63459013-63459032 None:intergenic 60.0%
TGTTGCTGAGGCCGAGGTGA+AGG - chr4.1:63460232-63460251 None:intergenic 60.0%
! ATAGACCGCACCGGTAGAGG+CGG + chr4.1:63459707-63459726 MS.gene72520:intron 60.0%
! CCTATAGACCGCACCGGTAG+AGG + chr4.1:63459704-63459723 MS.gene72520:intron 60.0%
!! CCGGATTGAAGTGCCGGACT+AGG - chr4.1:63459601-63459620 None:intergenic 60.0%
AGCTAGACCCCCGCCTCTAC+CGG - chr4.1:63459720-63459739 None:intergenic 65.0%
CACCGCGAGCGGTGGAAGAA+AGG + chr4.1:63460095-63460114 MS.gene72520:intron 65.0%
CGCCTATGTTGCTGAGGCCG+AGG - chr4.1:63460238-63460257 None:intergenic 65.0%
GAGGCCGAGACTCCTGCAGA+TGG - chr4.1:63459201-63459220 None:intergenic 65.0%
! TAGACCGCACCGGTAGAGGC+GGG + chr4.1:63459708-63459727 MS.gene72520:intron 65.0%
GCAGAGCCGTCTGAGCAGCG+AGG - chr4.1:63459125-63459144 None:intergenic 70.0%
TCTCGGTACACCGCGAGCGG+TGG + chr4.1:63460087-63460106 MS.gene72520:intron 70.0%
! AGACCGCACCGGTAGAGGCG+GGG + chr4.1:63459709-63459728 MS.gene72520:intron 70.0%
GACCCCCGCCTCTACCGGTG+CGG - chr4.1:63459715-63459734 None:intergenic 75.0%
! GACCGCACCGGTAGAGGCGG+GGG + chr4.1:63459710-63459729 MS.gene72520:intron 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 63458520 63460415 63458520 ID=MS.gene72520
chr4.1 mRNA 63458520 63460415 63458520 ID=MS.gene72520.t1;Parent=MS.gene72520
chr4.1 exon 63458520 63459478 63458520 ID=MS.gene72520.t1.exon1;Parent=MS.gene72520.t1
chr4.1 CDS 63458520 63459478 63458520 ID=cds.MS.gene72520.t1;Parent=MS.gene72520.t1
chr4.1 exon 63460328 63460415 63460328 ID=MS.gene72520.t1.exon2;Parent=MS.gene72520.t1
chr4.1 CDS 63460328 63460415 63460328 ID=cds.MS.gene72520.t1;Parent=MS.gene72520.t1
Gene Sequence

>MS.gene72520

ATGGAACGCATTGCTCCTGGTGTGGTTCCGATCACTGTTCCTCTGAATCATTTGGATATCTGGGTGCAGGTATATGGATTGCCCTTTGGTTTCATTCAATCAAAGGTTGGTCAAGGCATTGGCAGCTTTCTCGGTACTCTCCTAACCTATGATGGTCGCAACACCATTCATAGCTCCTATATGCGCCTCAAAGTCCGGATTGATGTCACTGCACCGCTGAAAAAGGAGTGGAAAGTTCGTGCTAGCAATGGGTCCTATGTTACTATTTTTTTCAAATATGAGAAACTGGGTGTTTTTTGTTACAGGTGTGGCATTCTCAGACATTCGGACAAGGTGTGTCCCGACTTGTTTGAATTGGACTCGGACGATGGCGTACGTAATTGGGGACCTGATCTCAAACCTGTTACGACTCGTATAGGGTCGGCTGCTACAAACAGATGGCTTCAGGATCCCATCCCTGATGTTGTGCCGCATCAAAGTAACTTCCAAGCCAGTTCTTCTGTTGGCCGCACCCATGCTGACAATTCTGTTTTGAATTTTAATAGCTTTAATAACCGTATGCTTGCTGTAGACACTGCTATTCATGCTATTAAACAGGATGTCCTCGCTGCTCAGACGGCTCTGCAAGCTAAACATGTCACAAATGGTGCTGGTTGTCAAGTTCCGATGCTTCCATCTCCATCTGCAGGAGTCTCGGCCTCAAATTTACTGCAGGGTCGTTCTATGGTACTTGGTTTGCCTGCTGCTCCTTTCACTTTTGCTGGTACAAATAATACTCACAGCGACAACGTTGTTCTTCTTGAGGATGCTGGTTCTGACTTGAAGAAACGCAAGTGTATGATCGCCCTTCAGAATGGAGAGTCCTCCTTTGCTGCTGATGGGGTTGGGGGAATTGATGTAATAGGATCCAATGTTAGTGCTGGGGGGGATTCTGAAATGCATGTAATTTCCAATCCTTTGTATGATGAAAAAGATGTAATGGCAGGACCTGATGATCAGGCCTGCCAAGGTAAATGAGTCTAATAGCGTGGAATTGTCGGGGTGTAGGTAGCCCGAGTACAATTCCCGATTTAAAGTACCTAGTCCGGCACTTCAATCCGGATCTTTTATTCCTAAGTGAGACACTAGCTCACCGAAATAAAATTGAAGAACTTCGTTTTTTGCTTGGTTACGATTCTTGTCTTCCTATAGACCGCACCGGTAGAGGCGGGGGTCTAGCTTTATTTTGGCGCAACTCTTTAAATTGTCACCTTATTGATTTTTCTAATAATCATATCACTGTTGAGATTGTGGATTCTGATCATGGTAATTGGAGAATGACCGGCTATTATGGCTATCCTAATGGAGGTCGTAGAACTGCCGCTTGGAATTTTCTTCGTCATCTCTCTAATCAATTTGCAGGCCCTTGGTGCATTTTTGGTGATTTCAATGATATTCTTGATGCTAGTGAGAAGAGGGGGCGCAATTCTAGACCACAGTGGCTCATCAATGGCTTTCGTGAAGCGGTTATCGACTCCGGTTTATCAGATGTTCAGGTAGAAGGCTATCCTTTCACATGGTTTAAAAGTCTCGGTACACCGCGAGCGGTGGAAGAAAGGTTAGACCGTGCCCTTGCTAACACTATGTGGTTTAATTTATTTCCGAATGCTACAGTTGAAACTCTAGTCGCACCTGCGTCTGATCACTATCCTATTTTATTAAAATGTTCTCCTTCACCTCGGCCTCAGCAACATAGGCGCAATTTTCGCTACGAAAATGCGTGGCAGTTAGAACCGGGGTTTAAGGATCTTGTTACTAACTCATGGCAGGTTCACTCTTCGCATACTCTTGTTCCAAAGCTTGTTGCGTGCGCAGAAGATATGGATGCTTGGCGCAAAACTCATTGCCATCAATTAA
Protein sequence

>MS.gene72520.t1

MERIAPGVVPITVPLNHLDIWVQVYGLPFGFIQSKVGQGIGSFLGTLLTYDGRNTIHSSYMRLKVRIDVTAPLKKEWKVRASNGSYVTIFFKYEKLGVFCYRCGILRHSDKVCPDLFELDSDDGVRNWGPDLKPVTTRIGSAATNRWLQDPIPDVVPHQSNFQASSSVGRTHADNSVLNFNSFNNRMLAVDTAIHAIKQDVLAAQTALQAKHVTNGAGCQVPMLPSPSAGVSASNLLQGRSMVLGLPAAPFTFAGTNNTHSDNVVLLEDAGSDLKKRKCMIALQNGESSFAADGVGGIDVIGSNVSAGGDSEMHVISNPLFTLRILLFQSLLRAQKIWMLGAKLIAIN