AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059736

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059736.t1 MTR_1g036720 55.455 110 49 0 1 110 1 110 7.81e-39 133
MS.gene059736.t1 MTR_0010s0370 50.909 110 54 0 1 110 1 110 9.63e-33 120
MS.gene059736.t1 MTR_0010s0350 50.909 110 54 0 1 110 1 110 9.63e-33 120
MS.gene059736.t1 MTR_1g051330 70.213 47 14 0 276 322 323 369 1.30e-14 75.1
MS.gene059736.t1 MTR_2g061500 27.358 106 72 1 9 109 11 116 3.64e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTGTTTGGCGAATTAAAT+TGG 0.105138 2.4:-20359845 None:intergenic
CATCGATCATGGCAGAATTT+TGG 0.212217 2.4:+20359093 MS.gene059736:CDS
CCAAGGTCCTTTCTTCAATA+TGG 0.223343 2.4:-20359198 None:intergenic
CCCTCATGCTTCAACTTTCA+TGG 0.238566 2.4:+20359891 MS.gene059736:CDS
GTCAGCCAATGTAACCGTTT+TGG 0.280239 2.4:-20359809 None:intergenic
GTCGTTCAACAACGCAATTC+CGG 0.281101 2.4:+20359649 MS.gene059736:CDS
TAACCATCTTGAACCTTTGC+CGG 0.323890 2.4:-20359668 None:intergenic
CTTCCTCATCACTCAGATAT+AGG 0.346648 2.4:-20358962 None:intergenic
TTCTGATGAACCTTAACTAA+TGG 0.354381 2.4:-20359718 None:intergenic
CAAAGGAAACCCGTCTTGAT+AGG 0.356044 2.4:+20360744 MS.gene059736:intron
AGATGAATTGGATGACTCTT+TGG 0.362459 2.4:-20360043 None:intergenic
ATTACCATTCAGATCATTCC+TGG 0.367026 2.4:-20359956 None:intergenic
CAGAAAGTCGACATCACTTC+CGG 0.371993 2.4:+20359736 MS.gene059736:CDS
CTGGGAGAATGTCGAGAATC+AGG 0.383690 2.4:-20359238 None:intergenic
AATTTGAATGTGAAGAGGTT+TGG 0.404612 2.4:-20359152 None:intergenic
TGGCGTGATTACTGACTTCA+TGG 0.406453 2.4:-20360010 None:intergenic
CACTGATACGAGCGCAAGTC+TGG 0.408042 2.4:+20359025 MS.gene059736:CDS
TGAATGGGAATTTAATGTCT+TGG 0.410463 2.4:-20359779 None:intergenic
TGGGGAAGATGAAGATGAAT+TGG 0.415118 2.4:-20360055 None:intergenic
GCAAAGGTTCAAGATGGTTA+AGG 0.415876 2.4:+20359671 MS.gene059736:CDS
CTCGTATCAGTGGAGGAATT+AGG 0.418541 2.4:-20359014 None:intergenic
GACATCACTTCCGGCGGAAT+CGG 0.419183 2.4:+20359745 MS.gene059736:CDS
CACGTTGTGAAGTCTCCATT+GGG 0.422606 2.4:-20360074 None:intergenic
GCTGTTTGGCGAATTAAATT+GGG 0.443010 2.4:-20359844 None:intergenic
TGTGTTTATTGCTCGCTGTT+TGG 0.444024 2.4:-20359858 None:intergenic
TGTTGAACGACTCTTAGTTC+AGG 0.444781 2.4:-20359637 None:intergenic
TGATGATGGAGTTCGCCATT+GGG 0.453160 2.4:+20359612 MS.gene059736:CDS
AGGGTTTGAAGAGACGCTTT+AGG 0.453974 2.4:-20360800 None:intergenic
CTGGTCATCGCCATTAGTTA+AGG 0.454362 2.4:+20359708 MS.gene059736:CDS
CAAAGGTTCAAGATGGTTAA+GGG 0.462843 2.4:+20359672 MS.gene059736:CDS
CTGATGATGGAGTTCGCCAT+TGG 0.479009 2.4:+20359611 MS.gene059736:CDS
TGGTTTGCATATCACGGTAA+TGG 0.479641 2.4:-20359178 None:intergenic
CCATTCAATTGCTCCCAAAA+CGG 0.488342 2.4:+20359795 MS.gene059736:CDS
CCGGATCTGTCTGAAGGAGG+AGG 0.496785 2.4:-20360850 None:intergenic
TGCTCCCAAAACGGTTACAT+TGG 0.498301 2.4:+20359804 MS.gene059736:CDS
CAACAACGCAATTCCGGCAA+AGG 0.504305 2.4:+20359655 MS.gene059736:CDS
TCACGTTGTGAAGTCTCCAT+TGG 0.506168 2.4:-20360075 None:intergenic
CCTCCTCCTTCAGACAGATC+CGG 0.506603 2.4:+20360850 MS.gene059736:CDS
TCATCTTGCTGACTCATCTC+AGG 0.506696 2.4:+20359981 MS.gene059736:CDS
TAAGGGAGGACGGCCAAGAC+TGG 0.510281 2.4:+20359689 MS.gene059736:CDS
TATTATGTTGAAGCTGATGA+TGG 0.517402 2.4:+20359598 MS.gene059736:intron
CTACAAACTTGAAACAGTCC+AGG 0.519466 2.4:+20359938 MS.gene059736:CDS
ATTGGATGACTCTTTGGAAG+AGG 0.519737 2.4:-20360037 None:intergenic
ATGCAAACCATATTGAAGAA+AGG 0.522703 2.4:+20359191 MS.gene059736:CDS
TTATATGATGACATCGATCA+TGG 0.523154 2.4:+20359082 MS.gene059736:CDS
CGGATCTGTCTGAAGGAGGA+GGG 0.526173 2.4:-20360849 None:intergenic
CTAATGGCGATGACCAGTCT+TGG 0.526517 2.4:-20359702 None:intergenic
TCAATATGGTTTGCATATCA+CGG 0.528071 2.4:-20359184 None:intergenic
TTGATGTTTACTCCTCTACC+CGG 0.533090 2.4:-20359119 None:intergenic
CGTTACAGATAAACCTGTGA+GGG 0.537294 2.4:+20359058 MS.gene059736:CDS
TTGGATGACTCTTTGGAAGA+GGG 0.540084 2.4:-20360036 None:intergenic
TTCATCTTCATCTTCCCCAA+TGG 0.543136 2.4:+20360059 MS.gene059736:CDS
AAAGGAAACCCGTCTTGATA+GGG 0.544816 2.4:+20360745 MS.gene059736:intron
AAATAAGAGGAATACTCTGT+GGG 0.550472 2.4:-20359271 None:intergenic
CCATGAAAGTTGAAGCATGA+GGG 0.553127 2.4:-20359891 None:intergenic
TGTGGCATTCACCATGGGAC+CGG 0.557458 2.4:-20360869 None:intergenic
AACAAGAGCCCTATCAAGAC+GGG 0.560314 2.4:-20360753 None:intergenic
GACTCTTTGGAAGAGGGAGA+TGG 0.567286 2.4:-20360030 None:intergenic
TTCAGACAGATCCGGTCCCA+TGG 0.570275 2.4:+20360858 MS.gene059736:CDS
CAGTCCAGGAATGATCTGAA+TGG 0.573487 2.4:+20359952 MS.gene059736:CDS
TGATCTTGAGATTGCAATCG+AGG 0.576230 2.4:+20358986 MS.gene059736:CDS
CAGACTTGCGCTCGTATCAG+TGG 0.578627 2.4:-20359024 None:intergenic
ATTCCGGCAAAGGTTCAAGA+TGG 0.587810 2.4:+20359665 MS.gene059736:CDS
TAACAAGAGCCCTATCAAGA+CGG 0.591935 2.4:-20360754 None:intergenic
GTCATCATATAACCCCTCAC+AGG 0.597421 2.4:-20359071 None:intergenic
GGGCTCTTGTTAGCGTGTCG+TGG 0.603354 2.4:+20360765 MS.gene059736:CDS
GATACGAGCGCAAGTCTGGT+AGG 0.607548 2.4:+20359029 MS.gene059736:CDS
CCATATTGAAGAAAGGACCT+TGG 0.608392 2.4:+20359198 MS.gene059736:CDS
GGATCTGTCTGAAGGAGGAG+GGG 0.611235 2.4:-20360848 None:intergenic
TTGTGTGTGGCATTCACCAT+GGG 0.612968 2.4:-20360874 None:intergenic
TCAAGATGGTTAAGGGAGGA+CGG 0.613953 2.4:+20359679 MS.gene059736:CDS
GATCAGACATAGGTGCAACA+AGG 0.615353 2.4:-20360820 None:intergenic
GTTACTGTTTCCGATTCCGC+CGG 0.617480 2.4:-20359755 None:intergenic
AGGGGAGTGTGATCAGACAT+AGG 0.619796 2.4:-20360830 None:intergenic
TCCATGAAAGTTGAAGCATG+AGG 0.619951 2.4:-20359892 None:intergenic
CATGAAAGTTGAAGCATGAG+GGG 0.623455 2.4:-20359890 None:intergenic
ATCAGACATAGGTGCAACAA+GGG 0.623521 2.4:-20360819 None:intergenic
TGATGTTTACTCCTCTACCC+GGG 0.623585 2.4:-20359118 None:intergenic
ATGGGACCGGATCTGTCTGA+AGG 0.626902 2.4:-20360856 None:intergenic
CTTGTGTGTGGCATTCACCA+TGG 0.629775 2.4:-20360875 None:intergenic
CTCTTAGTTCAGGACCCCAA+TGG 0.632289 2.4:-20359627 None:intergenic
GGCGTGATTACTGACTTCAT+GGG 0.647194 2.4:-20360009 None:intergenic
AAACCTATATCTGAGTGATG+AGG 0.653560 2.4:+20358959 MS.gene059736:CDS
GATGATGGAGTTCGCCATTG+GGG 0.654076 2.4:+20359613 MS.gene059736:CDS
GGACCGGATCTGTCTGAAGG+AGG 0.654739 2.4:-20360853 None:intergenic
ATAAGAGGAATACTCTGTGG+GGG 0.672906 2.4:-20359269 None:intergenic
ACTTGCGCTCGTATCAGTGG+AGG 0.676334 2.4:-20359021 None:intergenic
GTTACAGATAAACCTGTGAG+GGG 0.678901 2.4:+20359059 MS.gene059736:CDS
GTGATTACTGACTTCATGGG+AGG 0.686319 2.4:-20360006 None:intergenic
AATAAGAGGAATACTCTGTG+GGG 0.695794 2.4:-20359270 None:intergenic
AGGTTCAAGATGGTTAAGGG+AGG 0.708166 2.4:+20359675 MS.gene059736:CDS
AAAGTCGACATCACTTCCGG+CGG 0.723049 2.4:+20359739 MS.gene059736:CDS
TCGTTACAGATAAACCTGTG+AGG 0.727726 2.4:+20359057 MS.gene059736:CDS
AGGTTATTATCAAAATACCA+AGG 0.728541 2.4:-20359215 None:intergenic
ACGTTGTGAAGTCTCCATTG+GGG 0.729290 2.4:-20360073 None:intergenic
GGAGAATGTCGAGAATCAGG+AGG 0.730148 2.4:-20359235 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACTTTATAGAAGACTTTT+TGG + chr2.4:20359367-20359386 MS.gene059736:intron 20.0%
! AGGTTATTATCAAAATACCA+AGG - chr2.4:20359218-20359237 None:intergenic 25.0%
!! ATTTTCTTCTGTCTTTTAAG+AGG - chr2.4:20359467-20359486 None:intergenic 25.0%
AAAATTCCCCAATCATTCAT+AGG - chr2.4:20359528-20359547 None:intergenic 30.0%
AAATAAGAGGAATACTCTGT+GGG - chr2.4:20359274-20359293 None:intergenic 30.0%
AATTTGAATGTGAAGAGGTT+TGG - chr2.4:20359155-20359174 None:intergenic 30.0%
ATCTTTCTTCAGCAGATAAA+AGG + chr2.4:20360594-20360613 MS.gene059736:intron 30.0%
ATGCAAACCATATTGAAGAA+AGG + chr2.4:20359191-20359210 MS.gene059736:CDS 30.0%
GGAAAAATTTGAATGTGAAG+AGG - chr2.4:20359160-20359179 None:intergenic 30.0%
GTGATGCTTCAAAAAATGTT+CGG + chr2.4:20360094-20360113 MS.gene059736:intron 30.0%
TATTATGTTGAAGCTGATGA+TGG + chr2.4:20359598-20359617 MS.gene059736:intron 30.0%
TCAAAAAGGATAACAATGTC+CGG - chr2.4:20360260-20360279 None:intergenic 30.0%
TCTTTCTTCAGCAGATAAAA+GGG + chr2.4:20360595-20360614 MS.gene059736:intron 30.0%
TGAATGGGAATTTAATGTCT+TGG - chr2.4:20359782-20359801 None:intergenic 30.0%
TGACAACATTATGAAAACCA+CGG - chr2.4:20360649-20360668 None:intergenic 30.0%
TTATATGATGACATCGATCA+TGG + chr2.4:20359082-20359101 MS.gene059736:CDS 30.0%
TTCTGATGAACCTTAACTAA+TGG - chr2.4:20359721-20359740 None:intergenic 30.0%
! CTAAGTTTGAAGTATTCCAT+TGG + chr2.4:20360305-20360324 MS.gene059736:intron 30.0%
! GGGGAATTTTTGTTACTATT+CGG + chr2.4:20359538-20359557 MS.gene059736:intron 30.0%
! TAAATTGGTAACCCGTTATT+GGG - chr2.4:20360694-20360713 None:intergenic 30.0%
! TCAATATGGTTTGCATATCA+CGG - chr2.4:20359187-20359206 None:intergenic 30.0%
! TCTTATTTCAGTTCCATTCT+GGG + chr2.4:20359286-20359305 MS.gene059736:intron 30.0%
!!! GTCGAACGATCATTTTTTAT+CGG + chr2.4:20360119-20360138 MS.gene059736:intron 30.0%
AAACCTATATCTGAGTGATG+AGG + chr2.4:20358959-20358978 MS.gene059736:CDS 35.0%
AATAAGAGGAATACTCTGTG+GGG - chr2.4:20359273-20359292 None:intergenic 35.0%
ACAGAAGAAAATTAAGCGTC+CGG + chr2.4:20359475-20359494 MS.gene059736:intron 35.0%
AGATGAATTGGATGACTCTT+TGG - chr2.4:20360046-20360065 None:intergenic 35.0%
ATTACCATTCAGATCATTCC+TGG - chr2.4:20359959-20359978 None:intergenic 35.0%
CAAAGGTTCAAGATGGTTAA+GGG + chr2.4:20359672-20359691 MS.gene059736:CDS 35.0%
CAGAATGGAACTGAAATAAG+AGG - chr2.4:20359287-20359306 None:intergenic 35.0%
CTTTCTTCAGCAGATAAAAG+GGG + chr2.4:20360596-20360615 MS.gene059736:intron 35.0%
GAAATAAGAGGAATACTCTG+TGG - chr2.4:20359275-20359294 None:intergenic 35.0%
GAATTTCTGATATACCAGCA+GGG + chr2.4:20359312-20359331 MS.gene059736:intron 35.0%
GCTGTTTGGCGAATTAAATT+GGG - chr2.4:20359847-20359866 None:intergenic 35.0%
TAAGAGGATCAGTAACATCT+AGG - chr2.4:20359451-20359470 None:intergenic 35.0%
TGCTCTTAAACCAGGTAAAT+TGG - chr2.4:20360709-20360728 None:intergenic 35.0%
TGCTGCATGAAGTTTCAAAA+AGG - chr2.4:20360274-20360293 None:intergenic 35.0%
! AATGAGCAGAATTTTGCCAT+AGG - chr2.4:20360385-20360404 None:intergenic 35.0%
! CTCTTATTTCAGTTCCATTC+TGG + chr2.4:20359285-20359304 MS.gene059736:intron 35.0%
! GTAAATTGGTAACCCGTTAT+TGG - chr2.4:20360695-20360714 None:intergenic 35.0%
! TTGTTACTATTCGGCTTGTT+GGG + chr2.4:20359547-20359566 MS.gene059736:intron 35.0%
! TTTACCTGGTTTAAGAGCAT+TGG + chr2.4:20360710-20360729 MS.gene059736:intron 35.0%
! TTTGTTACTATTCGGCTTGT+TGG + chr2.4:20359546-20359565 MS.gene059736:intron 35.0%
AAACCACGGATAAGCCATAA+TGG - chr2.4:20360635-20360654 None:intergenic 40.0%
AAAGGAAACCCGTCTTGATA+GGG + chr2.4:20360745-20360764 MS.gene059736:intron 40.0%
AACCACGGATAAGCCATAAT+GGG - chr2.4:20360634-20360653 None:intergenic 40.0%
AAGACGCCAATTACCTTTCA+CGG - chr2.4:20360459-20360478 None:intergenic 40.0%
AAGTCATGAATGTCATAGCC+TGG + chr2.4:20360168-20360187 MS.gene059736:intron 40.0%
AAGTCTCCTATAACACACCA+AGG - chr2.4:20360566-20360585 None:intergenic 40.0%
ACGGACAAGGTCTTTAATAC+TGG - chr2.4:20360228-20360247 None:intergenic 40.0%
ACTACCAATGCTCTTAAACC+AGG - chr2.4:20360717-20360736 None:intergenic 40.0%
AGGGTTTATGGCAGAAAAAG+TGG + chr2.4:20359331-20359350 MS.gene059736:intron 40.0%
ATAAGAGGAATACTCTGTGG+GGG - chr2.4:20359272-20359291 None:intergenic 40.0%
ATCAGACATAGGTGCAACAA+GGG - chr2.4:20360822-20360841 None:intergenic 40.0%
CAATGTCCGGTTTATAGACA+CGG - chr2.4:20360247-20360266 None:intergenic 40.0%
CAGAAGAAAATTAAGCGTCC+GGG + chr2.4:20359476-20359495 MS.gene059736:intron 40.0%
CATGAAAGTTGAAGCATGAG+GGG - chr2.4:20359893-20359912 None:intergenic 40.0%
CCAAGGTCCTTTCTTCAATA+TGG - chr2.4:20359201-20359220 None:intergenic 40.0%
CCATATTGAAGAAAGGACCT+TGG + chr2.4:20359198-20359217 MS.gene059736:CDS 40.0%
CCATGAAAGTTGAAGCATGA+GGG - chr2.4:20359894-20359913 None:intergenic 40.0%
CCATTCAATTGCTCCCAAAA+CGG + chr2.4:20359795-20359814 MS.gene059736:CDS 40.0%
CCTTAACATTTCCTAAGCCT+CGG - chr2.4:20360199-20360218 None:intergenic 40.0%
CGAATTTCTGATATACCAGC+AGG + chr2.4:20359311-20359330 MS.gene059736:intron 40.0%
CGCTGTTTGGCGAATTAAAT+TGG - chr2.4:20359848-20359867 None:intergenic 40.0%
CGCTTCACCTATGAATGATT+GGG + chr2.4:20359518-20359537 MS.gene059736:intron 40.0%
CGGACAAGGTCTTTAATACT+GGG - chr2.4:20360227-20360246 None:intergenic 40.0%
CGTTACAGATAAACCTGTGA+GGG + chr2.4:20359058-20359077 MS.gene059736:CDS 40.0%
CTACAAACTTGAAACAGTCC+AGG + chr2.4:20359938-20359957 MS.gene059736:CDS 40.0%
CTTCCTCATCACTCAGATAT+AGG - chr2.4:20358965-20358984 None:intergenic 40.0%
GAAGTGGTGATCAAAACCAA+TGG - chr2.4:20360324-20360343 None:intergenic 40.0%
GCAAAGGTTCAAGATGGTTA+AGG + chr2.4:20359671-20359690 MS.gene059736:CDS 40.0%
GCTATGACATTCATGACTTC+CGG - chr2.4:20360167-20360186 None:intergenic 40.0%
GCTTCACCTATGAATGATTG+GGG + chr2.4:20359519-20359538 MS.gene059736:intron 40.0%
GTGATATGTCACATGAACCT+TGG + chr2.4:20360546-20360565 MS.gene059736:intron 40.0%
GTTACAGATAAACCTGTGAG+GGG + chr2.4:20359059-20359078 MS.gene059736:CDS 40.0%
TAACAAGAGCCCTATCAAGA+CGG - chr2.4:20360757-20360776 None:intergenic 40.0%
TAACCATCTTGAACCTTTGC+CGG - chr2.4:20359671-20359690 None:intergenic 40.0%
TATGGCAGAAAAAGTGGAGA+AGG + chr2.4:20359337-20359356 MS.gene059736:intron 40.0%
TCAGAAATTCGAACCCAGAA+TGG - chr2.4:20359302-20359321 None:intergenic 40.0%
TCCATGAAAGTTGAAGCATG+AGG - chr2.4:20359895-20359914 None:intergenic 40.0%
TCGCTTCACCTATGAATGAT+TGG + chr2.4:20359517-20359536 MS.gene059736:intron 40.0%
TCGTTACAGATAAACCTGTG+AGG + chr2.4:20359057-20359076 MS.gene059736:CDS 40.0%
TCTATTAGTGATGCCGTGAA+AGG + chr2.4:20360443-20360462 MS.gene059736:intron 40.0%
TGATATACCAGCAGGGTTTA+TGG + chr2.4:20359319-20359338 MS.gene059736:intron 40.0%
TGATCTTGAGATTGCAATCG+AGG + chr2.4:20358986-20359005 MS.gene059736:CDS 40.0%
TGGGGAAGATGAAGATGAAT+TGG - chr2.4:20360058-20360077 None:intergenic 40.0%
TGTGTTTATTGCTCGCTGTT+TGG - chr2.4:20359861-20359880 None:intergenic 40.0%
TGTTGAACGACTCTTAGTTC+AGG - chr2.4:20359640-20359659 None:intergenic 40.0%
TTCATCTTCATCTTCCCCAA+TGG + chr2.4:20360059-20360078 MS.gene059736:CDS 40.0%
TTCTATGGTTACCCTGACAA+TGG + chr2.4:20360485-20360504 MS.gene059736:intron 40.0%
TTGATGTTTACTCCTCTACC+CGG - chr2.4:20359122-20359141 None:intergenic 40.0%
! ATTGGATGACTCTTTGGAAG+AGG - chr2.4:20360040-20360059 None:intergenic 40.0%
! CATCGATCATGGCAGAATTT+TGG + chr2.4:20359093-20359112 MS.gene059736:CDS 40.0%
! CATGAACCTTGGTGTGTTAT+AGG + chr2.4:20360557-20360576 MS.gene059736:intron 40.0%
! TCAGCCAATGTAACCGTTTT+GGG - chr2.4:20359811-20359830 None:intergenic 40.0%
! TGGTTTGCATATCACGGTAA+TGG - chr2.4:20359181-20359200 None:intergenic 40.0%
! TTGGATGACTCTTTGGAAGA+GGG - chr2.4:20360039-20360058 None:intergenic 40.0%
!! CACTTCTCTGTTGATAGCAT+CGG + chr2.4:20360338-20360357 MS.gene059736:intron 40.0%
!! TAACGGGTTACCAATTTACC+TGG + chr2.4:20360696-20360715 MS.gene059736:intron 40.0%
!! TGTTACTATTCGGCTTGTTG+GGG + chr2.4:20359548-20359567 MS.gene059736:intron 40.0%
!!! CGTTTTGGGAGCAATTGAAT+GGG - chr2.4:20359797-20359816 None:intergenic 40.0%
AACAAGAGCCCTATCAAGAC+GGG - chr2.4:20360756-20360775 None:intergenic 45.0%
AAGAAAATTAAGCGTCCGGG+AGG + chr2.4:20359479-20359498 MS.gene059736:intron 45.0%
ACGTTGTGAAGTCTCCATTG+GGG - chr2.4:20360076-20360095 None:intergenic 45.0%
AGAAAATTAAGCGTCCGGGA+GGG + chr2.4:20359480-20359499 MS.gene059736:intron 45.0%
AGAACAATGCCAATCACCTC+AGG + chr2.4:20359402-20359421 MS.gene059736:intron 45.0%
AGGGTTTGAAGAGACGCTTT+AGG - chr2.4:20360803-20360822 None:intergenic 45.0%
AGGTTCAAGATGGTTAAGGG+AGG + chr2.4:20359675-20359694 MS.gene059736:CDS 45.0%
ATCACCTCAGGTCATACATG+CGG + chr2.4:20359414-20359433 MS.gene059736:intron 45.0%
ATGGAAGACGACGTCAATCT+TGG + chr2.4:20360504-20360523 MS.gene059736:intron 45.0%
ATTCCGGCAAAGGTTCAAGA+TGG + chr2.4:20359665-20359684 MS.gene059736:CDS 45.0%
CAAAGGAAACCCGTCTTGAT+AGG + chr2.4:20360744-20360763 MS.gene059736:intron 45.0%
CACGTTGTGAAGTCTCCATT+GGG - chr2.4:20360077-20360096 None:intergenic 45.0%
CAGAAAGTCGACATCACTTC+CGG + chr2.4:20359736-20359755 MS.gene059736:CDS 45.0%
CAGTCCAGGAATGATCTGAA+TGG + chr2.4:20359952-20359971 MS.gene059736:CDS 45.0%
CATGTATGACCTGAGGTGAT+TGG - chr2.4:20359414-20359433 None:intergenic 45.0%
CCCTCATGCTTCAACTTTCA+TGG + chr2.4:20359891-20359910 MS.gene059736:CDS 45.0%
CCGAGGCTTAGGAAATGTTA+AGG + chr2.4:20360196-20360215 MS.gene059736:intron 45.0%
CCTTGTCCGTGTCTATAAAC+CGG + chr2.4:20360238-20360257 MS.gene059736:intron 45.0%
CCTTTGTGACCTCCCAATAA+CGG + chr2.4:20360679-20360698 MS.gene059736:intron 45.0%
CGATGCTATCAACAGAGAAG+TGG - chr2.4:20360340-20360359 None:intergenic 45.0%
CTCGTATCAGTGGAGGAATT+AGG - chr2.4:20359017-20359036 None:intergenic 45.0%
CTGGTCATCGCCATTAGTTA+AGG + chr2.4:20359708-20359727 MS.gene059736:CDS 45.0%
CTTTGTGACCTCCCAATAAC+GGG + chr2.4:20360680-20360699 MS.gene059736:intron 45.0%
GAGGTGATTGGCATTGTTCT+TGG - chr2.4:20359402-20359421 None:intergenic 45.0%
GATCAGACATAGGTGCAACA+AGG - chr2.4:20360823-20360842 None:intergenic 45.0%
GTCAGGGTAACCATAGAATG+CGG - chr2.4:20360483-20360502 None:intergenic 45.0%
GTCATCATATAACCCCTCAC+AGG - chr2.4:20359074-20359093 None:intergenic 45.0%
GTCGTTCAACAACGCAATTC+CGG + chr2.4:20359649-20359668 MS.gene059736:CDS 45.0%
GTGATGCCGTGAAAGGTAAT+TGG + chr2.4:20360450-20360469 MS.gene059736:intron 45.0%
TCAAGATGGTTAAGGGAGGA+CGG + chr2.4:20359679-20359698 MS.gene059736:CDS 45.0%
TCACGTTGTGAAGTCTCCAT+TGG - chr2.4:20360078-20360097 None:intergenic 45.0%
TCTTCAGCAGATAAAAGGGG+AGG + chr2.4:20360599-20360618 MS.gene059736:intron 45.0%
TGATGTTTACTCCTCTACCC+GGG - chr2.4:20359121-20359140 None:intergenic 45.0%
TGCTCCCAAAACGGTTACAT+TGG + chr2.4:20359804-20359823 MS.gene059736:CDS 45.0%
TGGAAGACGACGTCAATCTT+GGG + chr2.4:20360505-20360524 MS.gene059736:intron 45.0%
! ATTGGTAACCCGTTATTGGG+AGG - chr2.4:20360691-20360710 None:intergenic 45.0%
! CATTGGTAGTAGCCAAGCAA+AGG + chr2.4:20360727-20360746 MS.gene059736:intron 45.0%
! GTCAGCCAATGTAACCGTTT+TGG - chr2.4:20359812-20359831 None:intergenic 45.0%
! TTTTCTGCCATAAACCCTGC+TGG - chr2.4:20359329-20359348 None:intergenic 45.0%
!! ATAAGCCATAATGGGCGATC+TGG - chr2.4:20360626-20360645 None:intergenic 45.0%
!! GGCGTGATTACTGACTTCAT+GGG - chr2.4:20360012-20360031 None:intergenic 45.0%
!! GTGATTACTGACTTCATGGG+AGG - chr2.4:20360009-20360028 None:intergenic 45.0%
!! GTTGATAGCATCGGACATAG+TGG + chr2.4:20360347-20360366 MS.gene059736:intron 45.0%
!! TCATCTTGCTGACTCATCTC+AGG + chr2.4:20359981-20360000 MS.gene059736:CDS 45.0%
!! TGATGATGGAGTTCGCCATT+GGG + chr2.4:20359612-20359631 MS.gene059736:CDS 45.0%
!! TGGCGTGATTACTGACTTCA+TGG - chr2.4:20360013-20360032 None:intergenic 45.0%
!!! CCGTTTTGGGAGCAATTGAA+TGG - chr2.4:20359798-20359817 None:intergenic 45.0%
AAAGTCGACATCACTTCCGG+CGG + chr2.4:20359739-20359758 MS.gene059736:CDS 50.0%
ACTGTATGTGATTCCCCTCC+CGG - chr2.4:20359497-20359516 None:intergenic 50.0%
CAACAACGCAATTCCGGCAA+AGG + chr2.4:20359655-20359674 MS.gene059736:CDS 50.0%
CCGGTTTATAGACACGGACA+AGG - chr2.4:20360241-20360260 None:intergenic 50.0%
CCGTTATTGGGAGGTCACAA+AGG - chr2.4:20360682-20360701 None:intergenic 50.0%
CTAATGGCGATGACCAGTCT+TGG - chr2.4:20359705-20359724 None:intergenic 50.0%
CTCTTAGTTCAGGACCCCAA+TGG - chr2.4:20359630-20359649 None:intergenic 50.0%
CTGGGAGAATGTCGAGAATC+AGG - chr2.4:20359241-20359260 None:intergenic 50.0%
GAAAATTAAGCGTCCGGGAG+GGG + chr2.4:20359481-20359500 MS.gene059736:intron 50.0%
GACGTCGTCTTCCATTGTCA+GGG - chr2.4:20360499-20360518 None:intergenic 50.0%
GGAGAATGTCGAGAATCAGG+AGG - chr2.4:20359238-20359257 None:intergenic 50.0%
GTTACTGTTTCCGATTCCGC+CGG - chr2.4:20359758-20359777 None:intergenic 50.0%
TGACATTCATGACTTCCGGC+AGG - chr2.4:20360163-20360182 None:intergenic 50.0%
TGACGTCGTCTTCCATTGTC+AGG - chr2.4:20360500-20360519 None:intergenic 50.0%
TGATCCGCATGTATGACCTG+AGG - chr2.4:20359421-20359440 None:intergenic 50.0%
TGGCGTCTTACCGCATTCTA+TGG + chr2.4:20360470-20360489 MS.gene059736:intron 50.0%
! AGGGGAGTGTGATCAGACAT+AGG - chr2.4:20360833-20360852 None:intergenic 50.0%
! CAAGACGGGTTTCCTTTGCT+TGG - chr2.4:20360742-20360761 None:intergenic 50.0%
! GACTCTTTGGAAGAGGGAGA+TGG - chr2.4:20360033-20360052 None:intergenic 50.0%
! GATAGCATCGGACATAGTGG+TGG + chr2.4:20360350-20360369 MS.gene059736:intron 50.0%
!! ATGGCAGAATTTTGGCACCC+GGG + chr2.4:20359101-20359120 MS.gene059736:CDS 50.0%
!! CATGGCAGAATTTTGGCACC+CGG + chr2.4:20359100-20359119 MS.gene059736:CDS 50.0%
!! CTGATGATGGAGTTCGCCAT+TGG + chr2.4:20359611-20359630 MS.gene059736:CDS 50.0%
!! GATGATGGAGTTCGCCATTG+GGG + chr2.4:20359613-20359632 MS.gene059736:CDS 50.0%
ACTTGCGCTCGTATCAGTGG+AGG - chr2.4:20359024-20359043 None:intergenic 55.0%
ATGGGACCGGATCTGTCTGA+AGG - chr2.4:20360859-20360878 None:intergenic 55.0%
CACTGATACGAGCGCAAGTC+TGG + chr2.4:20359025-20359044 MS.gene059736:CDS 55.0%
CAGACTTGCGCTCGTATCAG+TGG - chr2.4:20359027-20359046 None:intergenic 55.0%
CCTCCTCCTTCAGACAGATC+CGG + chr2.4:20360850-20360869 MS.gene059736:CDS 55.0%
CGCCCATTATGGCTTATCCG+TGG + chr2.4:20360629-20360648 MS.gene059736:intron 55.0%
CGGATCTGTCTGAAGGAGGA+GGG - chr2.4:20360852-20360871 None:intergenic 55.0%
GACATCACTTCCGGCGGAAT+CGG + chr2.4:20359745-20359764 MS.gene059736:CDS 55.0%
GAGGACCAGATCGCCCATTA+TGG + chr2.4:20360618-20360637 MS.gene059736:intron 55.0%
GATACGAGCGCAAGTCTGGT+AGG + chr2.4:20359029-20359048 MS.gene059736:CDS 55.0%
GGATCTGTCTGAAGGAGGAG+GGG - chr2.4:20360851-20360870 None:intergenic 55.0%
TTCAGACAGATCCGGTCCCA+TGG + chr2.4:20360858-20360877 MS.gene059736:CDS 55.0%
! TCTGTGGGGGCGTATTCTCT+GGG - chr2.4:20359259-20359278 None:intergenic 55.0%
!! CGGCTTGTTGGGGCATATAG+AGG + chr2.4:20359558-20359577 MS.gene059736:intron 55.0%
!! GAATTTTGGCACCCGGGTAG+AGG + chr2.4:20359107-20359126 MS.gene059736:CDS 55.0%
ATCGGCAGAGCCTGTAGAGC+AGG + chr2.4:20360137-20360156 MS.gene059736:intron 60.0%
CCGGATCTGTCTGAAGGAGG+AGG - chr2.4:20360853-20360872 None:intergenic 60.0%
GGACCGGATCTGTCTGAAGG+AGG - chr2.4:20360856-20360875 None:intergenic 60.0%
GGGCTCTTGTTAGCGTGTCG+TGG + chr2.4:20360765-20360784 MS.gene059736:CDS 60.0%
GTCATAGCCTGGAACTGCCG+AGG + chr2.4:20360179-20360198 MS.gene059736:intron 60.0%
TAAGGGAGGACGGCCAAGAC+TGG + chr2.4:20359689-20359708 MS.gene059736:CDS 60.0%
TCCTAAGCCTCGGCAGTTCC+AGG - chr2.4:20360189-20360208 None:intergenic 60.0%
! CTCTGTGGGGGCGTATTCTC+TGG - chr2.4:20359260-20359279 None:intergenic 60.0%
! GTGGTGGCGTTGCTGTCCTA+TGG + chr2.4:20360366-20360385 MS.gene059736:intron 60.0%
AGCCTGTAGAGCAGGCCTGC+CGG + chr2.4:20360145-20360164 MS.gene059736:intron 65.0%
GCCTGGAACTGCCGAGGCTT+AGG + chr2.4:20360185-20360204 MS.gene059736:intron 65.0%
TTCCGGCAGGCCTGCTCTAC+AGG - chr2.4:20360150-20360169 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 20358945 20360883 20358945 ID=MS.gene059736
chr2.4 mRNA 20358945 20360883 20358945 ID=MS.gene059736.t1;Parent=MS.gene059736
chr2.4 exon 20358945 20359278 20358945 ID=MS.gene059736.t1.exon1;Parent=MS.gene059736.t1
chr2.4 CDS 20358945 20359278 20358945 ID=cds.MS.gene059736.t1;Parent=MS.gene059736.t1
chr2.4 exon 20359611 20360110 20359611 ID=MS.gene059736.t1.exon2;Parent=MS.gene059736.t1
chr2.4 CDS 20359611 20360110 20359611 ID=cds.MS.gene059736.t1;Parent=MS.gene059736.t1
chr2.4 exon 20360749 20360883 20360749 ID=MS.gene059736.t1.exon3;Parent=MS.gene059736.t1
chr2.4 CDS 20360749 20360883 20360749 ID=cds.MS.gene059736.t1;Parent=MS.gene059736.t1
Gene Sequence

>MS.gene059736

ATGGCATCGCTTTCAAACCTATATCTGAGTGATGAGGAAGATGATCTTGAGATTGCAATCGAGGAGACTCCTAATTCCTCCACTGATACGAGCGCAAGTCTGGTAGGCAGATTCGTTACAGATAAACCTGTGAGGGGTTATATGATGACATCGATCATGGCAGAATTTTGGCACCCGGGTAGAGGAGTAAACATCAAAGAGATTGAACCAAACCTCTTCACATTCAAATTTTTCCATTACCGTGATATGCAAACCATATTGAAGAAAGGACCTTGGTATTTTGATAATAACCTCCTGATTCTCGACATTCTCCCAGAGAATACGCCCCCACAGAGTATTCCTCTTATTTCAGTTCCATTCTGGGTTCGAATTTCTGATATACCAGCAGGGTTTATGGCAGAAAAAGTGGAGAAGGATCTCGATAACTTTATAGAAGACTTTTTGGAGTACGACGCCAAGAACAATGCCAATCACCTCAGGTCATACATGCGGATCAGAGTGCTCCTAGATGTTACTGATCCTCTTAAAAGACAGAAGAAAATTAAGCGTCCGGGAGGGGAATCACATACAGTTCGCTTCACCTATGAATGATTGGGGAATTTTTGTTACTATTCGGCTTGTTGGGGCATATAGAGGACTATTATGATAAGCTCTATTATGTTGAAGCTGATGATGGAGTTCGCCATTGGGGTCCTGAACTAAGAGTCGTTCAACAACGCAATTCCGGCAAAGGTTCAAGATGGTTAAGGGAGGACGGCCAAGACTGGTCATCGCCATTAGTTAAGGTTCATCAGAAAGTCGACATCACTTCCGGCGGAATCGGAAACAGTAACGCCAAGACATTAAATTCCCATTCAATTGCTCCCAAAACGGTTACATTGGCTGACTTGCTAAAAAATCCCAATTTAATTCGCCAAACAGCGAGCAATAAACACACACATTCATCCCCTCATGCTTCAACTTTCATGGAAGAAGAAAATGCTGCAGTTTTTTCTACAAACTTGAAACAGTCCAGGAATGATCTGAATGGTAATAATCATCTTGCTGACTCATCTCAGGTTCCTCCCATGAAGTCAGTAATCACGCCATCTCCCTCTTCCAAAGAGTCATCCAATTCATCTTCATCTTCCCCAATGGAGACTTCACAACGTGATGCTTCAAAAAATGTTCGGTCGTCGAACGATCATTTTTTATCGGCAGAGCCTGTAGAGCAGGCCTGCCGGAAGTCATGAATGTCATAGCCTGGAACTGCCGAGGCTTAGGAAATGTTAAGGCAGATCCCAGTATTAAAGACCTTGTCCGTGTCTATAAACCGGACATTGTTATCCTTTTTGAAACTTCATGCAGCAATAATAAAATTCTAAGTTTGAAGTATTCCATTGGTTTTGATCACCACTTCTCTGTTGATAGCATCGGACATAGTGGTGGCGTTGCTGTCCTATGGCAAAATTCTGCTCATTATAATATTGTTAATTACTCTCAAAACTTTATTAAGATGTCTATTAGTGATGCCGTGAAAGGTAATTGGCGTCTTACCGCATTCTATGGTTACCCTGACAATGGAAGACGACGTCAATCTTGGGATCTACTTCGTTCTCTTCGTGATATGTCACATGAACCTTGGTGTGTTATAGGAGACTTCAATGATCATCTTTCTTCAGCAGATAAAAGGGGAGGACCAGATCGCCCATTATGGCTTATCCGTGGTTTTCATAATGTTGTCAATGATTGTAACCTTTGTGACCTCCCAATAACGGGTTACCAATTTACCTGGTTTAAGAGCATTGGTAGTAGCCAAGCAAAGGAAACCCGTCTTGATAGGGCTCTTGTTAGCGTGTCGTGGCATGATTTATTTCCTAAAGCGTCTCTTCAAACCCTTGTTGCACCTATGTCTGATCACACTCCCCTCCTCCTTCAGACAGATCCGGTCCCATGGTGA
Protein sequence

>MS.gene059736.t1

MASLSNLYLSDEEDDLEIAIEETPNSSTDTSASLVGRFVTDKPVRGYMMTSIMAEFWHPGRGVNIKEIEPNLFTFKFFHYRDMQTILKKGPWYFDNNLLILDILPENTPPQTDDGVRHWGPELRVVQQRNSGKGSRWLREDGQDWSSPLVKVHQKVDITSGGIGNSNAKTLNSHSIAPKTVTLADLLKNPNLIRQTASNKHTHSSPHASTFMEEENAAVFSTNLKQSRNDLNGNNHLADSSQVPPMKSVITPSPSSKESSNSSSSSPMETSQRDASKNETRLDRALVSVSWHDLFPKASLQTLVAPMSDHTPLLLQTDPVPW