AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041264

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041264.t1 MTR_1g036720 70.213 94 28 0 1 94 1 94 1.08e-43 144
MS.gene041264.t1 MTR_0010s0370 68.085 94 30 0 1 94 1 94 8.23e-39 134
MS.gene041264.t1 MTR_0010s0350 68.085 94 30 0 1 94 1 94 8.23e-39 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 67 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCCGGCTGCATTAATTT+TGG 0.169548 7.2:-66515370 None:intergenic
TCTGATTCCCTTGACCGTTT+TGG 0.283967 7.2:-66515475 None:intergenic
GCCTCTTTAATGCTGCTTTC+CGG 0.306120 7.2:-66515385 None:intergenic
TGATTCAGCAGTGTAGGTTT+TGG 0.314331 7.2:-66515326 None:intergenic
TGGAGAAGATTGAAACCTTC+TGG 0.317488 7.2:+66514648 MS.gene041264:CDS
GTTGGGAGATTTCATCAATC+TGG 0.343714 7.2:-66514553 None:intergenic
AATCAAGGTGATGGTTGAAT+TGG 0.371156 7.2:-66514724 None:intergenic
AACCTACACTGCTGAATCAT+TGG 0.386885 7.2:+66515330 MS.gene041264:CDS
TCAATATTCTTTGAAAATCA+AGG 0.391955 7.2:-66514739 None:intergenic
TAAACATAGGCGAGGGTCAC+TGG 0.404097 7.2:-66514579 None:intergenic
GACCCTCGCCTATGTTTAGT+TGG 0.443937 7.2:+66514584 MS.gene041264:CDS
TTGGTCGTTTCCACACAAAC+AGG 0.445308 7.2:+66514603 MS.gene041264:CDS
TGTCTTTCAACTCGCAGCTC+AGG 0.450742 7.2:-66515193 None:intergenic
GCGAGGGTCACTGGACTGTT+GGG 0.456098 7.2:-66514570 None:intergenic
ATGCTCCCATGACTGAGTTC+CGG 0.476995 7.2:-66515240 None:intergenic
TTCTTTGAAAATCAAGGTGA+TGG 0.483187 7.2:-66514733 None:intergenic
AAAATTCACGAAATCGAGCC+AGG 0.493437 7.2:+66514689 MS.gene041264:CDS
GGAGTTAATGCCATTACTGC+AGG 0.493771 7.2:+66515301 MS.gene041264:CDS
CAGACGACGGTAGTCGTTTG+TGG 0.499418 7.2:+66515167 MS.gene041264:intron
TGCTCGCCGGAACTCAGTCA+TGG 0.502002 7.2:+66515234 MS.gene041264:CDS
AGAGCTTCGAAAAGCTTCAA+TGG 0.502704 7.2:-66516603 None:intergenic
TTTGCATAGAGATCAATTGC+GGG 0.505559 7.2:-66516643 None:intergenic
GGCGAGGGTCACTGGACTGT+TGG 0.510499 7.2:-66514571 None:intergenic
ACTGTCGTGTGCCTCATTGA+TGG 0.517886 7.2:-66515593 None:intergenic
AAATCCAAAATTAATGCAGC+CGG 0.520106 7.2:+66515366 MS.gene041264:CDS
GTGCTTGCTGACGTGGGAGA+AGG 0.528725 7.2:-66515532 None:intergenic
GGTTGAATTGGAAAGTGTAT+AGG 0.530441 7.2:-66514712 None:intergenic
AATCTTCTCCATCATCATGT+AGG 0.533440 7.2:-66514636 None:intergenic
TTTCAAAGAATATTGAAGAA+GGG 0.533785 7.2:+66514746 MS.gene041264:CDS
ACTATTCCTTGCATCATCAA+TGG 0.539174 7.2:-66515557 None:intergenic
AGACGACGGTAGTCGTTTGT+GGG 0.543976 7.2:+66515168 MS.gene041264:intron
ACACTGGCGGAGCTGCTCGC+CGG 0.546594 7.2:+66515221 MS.gene041264:CDS
AATCCGCTCCTACATGATGA+TGG 0.548343 7.2:+66514628 MS.gene041264:CDS
ATCATCATGTAGGAGCGGAT+TGG 0.550563 7.2:-66514626 None:intergenic
GCCGGAAAGCAGCATTAAAG+AGG 0.558919 7.2:+66515384 MS.gene041264:CDS
AATTGGAAAGTGTATAGGCC+TGG 0.561277 7.2:-66514707 None:intergenic
GCTCGCCGGAACTCAGTCAT+GGG 0.561580 7.2:+66515235 MS.gene041264:CDS
GAAAGCAGCATTAAAGAGGC+AGG 0.563686 7.2:+66515388 MS.gene041264:CDS
TCATTGATGGTAGGACTGGT+AGG 0.563955 7.2:-66515580 None:intergenic
GAATATTGAAGAAGGGTCCA+TGG 0.567601 7.2:+66514753 MS.gene041264:CDS
ACCTTCTGGAACCCTGTCAA+AGG 0.571400 7.2:+66514662 MS.gene041264:CDS
CAGCAAACCAAAACGGTCAA+GGG 0.577403 7.2:+66515468 MS.gene041264:CDS
TGCAGTAATGGCATTAACTC+CGG 0.577775 7.2:-66515299 None:intergenic
CCAGCAAACCAAAACGGTCA+AGG 0.579632 7.2:+66515467 MS.gene041264:CDS
CACCAATGATTCAGCAGTGT+AGG 0.580780 7.2:-66515332 None:intergenic
AATGGTAGTGCTTGCTGACG+TGG 0.593117 7.2:-66515539 None:intergenic
AGCGGATTGGCCTGTTTGTG+TGG 0.593277 7.2:-66514613 None:intergenic
ACCTTTGACAGGGTTCCAGA+AGG 0.598687 7.2:-66514663 None:intergenic
GCACTACCATTGATGATGCA+AGG 0.600561 7.2:+66515551 MS.gene041264:CDS
TGCCTCATTGATGGTAGGAC+TGG 0.601680 7.2:-66515584 None:intergenic
TTATCACCAGCAAACCAAAA+CGG 0.606800 7.2:+66515461 MS.gene041264:CDS
TCGTGTGCCTCATTGATGGT+AGG 0.618221 7.2:-66515589 None:intergenic
CGACCAACTAAACATAGGCG+AGG 0.629546 7.2:-66514587 None:intergenic
GAGTTAATGCCATTACTGCA+GGG 0.633405 7.2:+66515302 MS.gene041264:CDS
GTGTCTGCAACTGTAACTGA+TGG 0.638117 7.2:-66515277 None:intergenic
GTTGAAAGACAAAAGAACAC+TGG 0.640542 7.2:+66515205 MS.gene041264:CDS
GCTTAGTGACAGATGATGCA+AGG 0.641043 7.2:-66514507 None:intergenic
GGAAACGACCAACTAAACAT+AGG 0.643420 7.2:-66514592 None:intergenic
ATGGTAGTGCTTGCTGACGT+GGG 0.646686 7.2:-66515538 None:intergenic
TCAGTTACAGTTGCAGACAC+CGG 0.648958 7.2:+66515280 MS.gene041264:CDS
TCTCCATCATCATGTAGGAG+CGG 0.665389 7.2:-66514631 None:intergenic
GACGACGGTAGTCGTTTGTG+GGG 0.665822 7.2:+66515169 MS.gene041264:intron
GACCAACTAAACATAGGCGA+GGG 0.667268 7.2:-66514586 None:intergenic
TACCAGTCCTACCATCAATG+AGG 0.690383 7.2:+66515582 MS.gene041264:CDS
TCATCTGTCACTAAGCGATG+AGG 0.692392 7.2:+66514514 MS.gene041264:CDS
GAAAGACAAAAGAACACTGG+CGG 0.693945 7.2:+66515208 MS.gene041264:CDS
ACACTGCTGAATCATTGGTG+CGG 0.744721 7.2:+66515335 MS.gene041264:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACATCAATTCATTTTTTAT+CGG + chr7.2:66515642-66515661 MS.gene041264:intron 15.0%
!! TTTCAAAGAATATTGAAGAA+GGG + chr7.2:66514746-66514765 MS.gene041264:CDS 20.0%
!!! TCAATATTCTTTGAAAATCA+AGG - chr7.2:66514742-66514761 None:intergenic 20.0%
!!! TTTTCAAAGAATATTGAAGA+AGG + chr7.2:66514745-66514764 MS.gene041264:CDS 20.0%
! AAAGTCAAAGATCAGATTAA+CGG + chr7.2:66515966-66515985 MS.gene041264:intron 25.0%
!! AAAATCTGATCCCAAATTTT+CGG + chr7.2:66516555-66516574 MS.gene041264:intron 25.0%
!! TAATCTGATCTTTGACTTTA+AGG - chr7.2:66515965-66515984 None:intergenic 25.0%
!!! CATTTTACTGTTGATAGAAT+TGG + chr7.2:66515861-66515880 MS.gene041264:intron 25.0%
!!! TTTAAGGTTGACAAAGTTTT+GGG - chr7.2:66515949-66515968 None:intergenic 25.0%
AAAAAGATTAAACGACATGG+AGG + chr7.2:66515034-66515053 MS.gene041264:intron 30.0%
AAAAGATTAAACGACATGGA+GGG + chr7.2:66515035-66515054 MS.gene041264:intron 30.0%
AAATCCAAAATTAATGCAGC+CGG + chr7.2:66515366-66515385 MS.gene041264:CDS 30.0%
AAATTCAAATACGAACGTCT+AGG + chr7.2:66515073-66515092 MS.gene041264:intron 30.0%
ATCACTTGGTCAAATTGAAT+TGG + chr7.2:66516462-66516481 MS.gene041264:intron 30.0%
CAGAAAAAGATTAAACGACA+TGG + chr7.2:66515031-66515050 MS.gene041264:intron 30.0%
CATTCACTAAAGTTTCGATA+AGG - chr7.2:66515797-66515816 None:intergenic 30.0%
GCGCAATGAATATAATATCT+TGG + chr7.2:66515691-66515710 MS.gene041264:intron 30.0%
TATTCTTTGCATCATATTCG+AGG - chr7.2:66514943-66514962 None:intergenic 30.0%
TCACTTGGTCAAATTGAATT+GGG + chr7.2:66516463-66516482 MS.gene041264:intron 30.0%
TCTGAATTATGCTACAACAT+TGG + chr7.2:66515828-66515847 MS.gene041264:intron 30.0%
! TTCACATGGTTCAAAAGTAT+CGG + chr7.2:66516233-66516252 MS.gene041264:intron 30.0%
! TTCTTTGAAAATCAAGGTGA+TGG - chr7.2:66514736-66514755 None:intergenic 30.0%
!! CATGTCGTTTAATCTTTTTC+TGG - chr7.2:66515033-66515052 None:intergenic 30.0%
!! GAATTTTGATACCTTTGACA+GGG - chr7.2:66514676-66514695 None:intergenic 30.0%
!! TGAATTTTGATACCTTTGAC+AGG - chr7.2:66514677-66514696 None:intergenic 30.0%
!!! CTTTAAGGTTGACAAAGTTT+TGG - chr7.2:66515950-66515969 None:intergenic 30.0%
AAAGATTAAACGACATGGAG+GGG + chr7.2:66515036-66515055 MS.gene041264:intron 35.0%
AATCTTCTCCATCATCATGT+AGG - chr7.2:66514639-66514658 None:intergenic 35.0%
ACTATTCCTTGCATCATCAA+TGG - chr7.2:66515560-66515579 None:intergenic 35.0%
AGCTTCGTTATAACATCAGA+AGG - chr7.2:66516500-66516519 None:intergenic 35.0%
ATGTATGTGAAATCTCCATG+GGG - chr7.2:66515627-66515646 None:intergenic 35.0%
CTTGATATCATTCCAGAGAA+CGG + chr7.2:66514800-66514819 MS.gene041264:intron 35.0%
GAATGATATCAAGAACCAAG+AGG - chr7.2:66514793-66514812 None:intergenic 35.0%
GATATTATATTCATTGCGCC+CGG - chr7.2:66515690-66515709 None:intergenic 35.0%
GATGTATGTGAAATCTCCAT+GGG - chr7.2:66515628-66515647 None:intergenic 35.0%
GGATACATGACAGAAAAAGT+CGG + chr7.2:66514887-66514906 MS.gene041264:intron 35.0%
GTCTGAGTATCCATAAAAAG+CGG - chr7.2:66516006-66516025 None:intergenic 35.0%
GTTGAAAGACAAAAGAACAC+TGG + chr7.2:66515205-66515224 MS.gene041264:CDS 35.0%
TCAGATCCTACATGAAAATC+CGG + chr7.2:66514975-66514994 MS.gene041264:intron 35.0%
TCGATAAGGATAACAATGTC+CGG - chr7.2:66515783-66515802 None:intergenic 35.0%
TCGTTATAACATCAGAAGGA+GGG - chr7.2:66516496-66516515 None:intergenic 35.0%
TGATGAGTTGCCGAAAATTT+GGG - chr7.2:66516568-66516587 None:intergenic 35.0%
TGATGTATGTGAAATCTCCA+TGG - chr7.2:66515629-66515648 None:intergenic 35.0%
TTATCACCAGCAAACCAAAA+CGG + chr7.2:66515461-66515480 MS.gene041264:CDS 35.0%
TTCGTTATAACATCAGAAGG+AGG - chr7.2:66516497-66516516 None:intergenic 35.0%
TTGATGAGTTGCCGAAAATT+TGG - chr7.2:66516569-66516588 None:intergenic 35.0%
TTTCTGTCATGTATCCTGTT+GGG - chr7.2:66514883-66514902 None:intergenic 35.0%
! AATCAAGGTGATGGTTGAAT+TGG - chr7.2:66514727-66514746 None:intergenic 35.0%
! GGTAATTATTGCTACTTCTG+TGG + chr7.2:66515094-66515113 MS.gene041264:intron 35.0%
! GGTTGAATTGGAAAGTGTAT+AGG - chr7.2:66514715-66514734 None:intergenic 35.0%
! GTGAATTGATAACCAGTCAA+GGG - chr7.2:66516218-66516237 None:intergenic 35.0%
! GTTGATAGAATTGGTAGAAG+CGG + chr7.2:66515870-66515889 MS.gene041264:intron 35.0%
! TGAATTGATAACCAGTCAAG+GGG - chr7.2:66516217-66516236 None:intergenic 35.0%
! TGACTGGTTATCAATTCACA+TGG + chr7.2:66516219-66516238 MS.gene041264:intron 35.0%
! TGTGAATTGATAACCAGTCA+AGG - chr7.2:66516219-66516238 None:intergenic 35.0%
! TTTTATGGATACTCAGACTC+AGG + chr7.2:66516008-66516027 MS.gene041264:intron 35.0%
! TTTTCTGTCATGTATCCTGT+TGG - chr7.2:66514884-66514903 None:intergenic 35.0%
AAAATTCACGAAATCGAGCC+AGG + chr7.2:66514689-66514708 MS.gene041264:CDS 40.0%
AAAGTCTCCTATTATGCACC+AGG - chr7.2:66516090-66516109 None:intergenic 40.0%
AACCTACACTGCTGAATCAT+TGG + chr7.2:66515330-66515349 MS.gene041264:CDS 40.0%
AAGATCAGATTAACGGCAAC+TGG + chr7.2:66515973-66515992 MS.gene041264:intron 40.0%
AAGTCTCCTATTATGCACCA+GGG - chr7.2:66516089-66516108 None:intergenic 40.0%
AGAGCTTCGAAAAGCTTCAA+TGG - chr7.2:66516606-66516625 None:intergenic 40.0%
AGAGTTTGCAAGGTTGCATT+CGG - chr7.2:66516326-66516345 None:intergenic 40.0%
AGGGATATCGCAAACTACAT+AGG + chr7.2:66514911-66514930 MS.gene041264:intron 40.0%
AGTTCCTACCATGAAAGACT+TGG + chr7.2:66515743-66515762 MS.gene041264:intron 40.0%
ATAATATCTTGGAACTGCCG+AGG + chr7.2:66515702-66515721 MS.gene041264:intron 40.0%
CAATGTCCGGTTTATAGACT+CGG - chr7.2:66515770-66515789 None:intergenic 40.0%
CAGATCCTACATGAAAATCC+GGG + chr7.2:66514976-66514995 MS.gene041264:intron 40.0%
GAAAGACAAAAGAACACTGG+CGG + chr7.2:66515208-66515227 MS.gene041264:CDS 40.0%
GAATATTGAAGAAGGGTCCA+TGG + chr7.2:66514753-66514772 MS.gene041264:CDS 40.0%
GAGTTAATGCCATTACTGCA+GGG + chr7.2:66515302-66515321 MS.gene041264:CDS 40.0%
GGAAACGACCAACTAAACAT+AGG - chr7.2:66514595-66514614 None:intergenic 40.0%
GGCAACTCATCAACAAACAA+AGG + chr7.2:66516576-66516595 MS.gene041264:intron 40.0%
GTACTTTGACAATCACCTCT+TGG + chr7.2:66514775-66514794 MS.gene041264:intron 40.0%
GTTAAACCGGAAACATCGAT+GGG - chr7.2:66516414-66516433 None:intergenic 40.0%
GTTGGGAGATTTCATCAATC+TGG - chr7.2:66514556-66514575 None:intergenic 40.0%
GTTTCCGGTTTAACAATGCT+TGG + chr7.2:66516420-66516439 MS.gene041264:intron 40.0%
TCATGGACTTGTATCCAGAA+TGG - chr7.2:66514857-66514876 None:intergenic 40.0%
TGAACCTGAACTGAATCACT+TGG + chr7.2:66516448-66516467 MS.gene041264:intron 40.0%
TGCAGTAATGGCATTAACTC+CGG - chr7.2:66515302-66515321 None:intergenic 40.0%
TGGAGAAGATTGAAACCTTC+TGG + chr7.2:66514648-66514667 MS.gene041264:CDS 40.0%
TGTATGTGAAATCTCCATGG+GGG - chr7.2:66515626-66515645 None:intergenic 40.0%
TGTTAAACCGGAAACATCGA+TGG - chr7.2:66516415-66516434 None:intergenic 40.0%
TTAAACCGGAAACATCGATG+GGG - chr7.2:66516413-66516432 None:intergenic 40.0%
TTGACCAAGTGATTCAGTTC+AGG - chr7.2:66516455-66516474 None:intergenic 40.0%
! AATTGGAAAGTGTATAGGCC+TGG - chr7.2:66514710-66514729 None:intergenic 40.0%
! AGGTGATTGTCAAAGTACCA+TGG - chr7.2:66514773-66514792 None:intergenic 40.0%
! CTTTCCGGCTGCATTAATTT+TGG - chr7.2:66515373-66515392 None:intergenic 40.0%
! GGATTTTCATGTAGGATCTG+AGG - chr7.2:66514976-66514995 None:intergenic 40.0%
! TGATTCAGCAGTGTAGGTTT+TGG - chr7.2:66515329-66515348 None:intergenic 40.0%
! TGGATGTCTAGGACATATTG+AGG + chr7.2:66515114-66515133 MS.gene041264:intron 40.0%
! TGGGATCAGATTTTTACTCC+AGG - chr7.2:66516549-66516568 None:intergenic 40.0%
!! ATCTTTTTCTGGCGCTTCAA+TGG - chr7.2:66515022-66515041 None:intergenic 40.0%
!!! TGGAGGTTAACCGCTTTTTA+TGG + chr7.2:66515993-66516012 MS.gene041264:intron 40.0%
AATCCGCTCCTACATGATGA+TGG + chr7.2:66514628-66514647 MS.gene041264:CDS 45.0%
ACACTGCTGAATCATTGGTG+CGG + chr7.2:66515335-66515354 MS.gene041264:CDS 45.0%
ACATGACAGAAAAAGTCGGC+AGG + chr7.2:66514891-66514910 MS.gene041264:intron 45.0%
AGAATGGTACGAAGTGGAGA+GGG - chr7.2:66514841-66514860 None:intergenic 45.0%
AGGAGCAACAAGAGTTTGCA+AGG - chr7.2:66516336-66516355 None:intergenic 45.0%
AGTGGCGTATGGTCTGATAT+AGG - chr7.2:66516356-66516375 None:intergenic 45.0%
ATCAGATTAACGGCAACTGG+AGG + chr7.2:66515976-66515995 MS.gene041264:intron 45.0%
ATCATCATGTAGGAGCGGAT+TGG - chr7.2:66514629-66514648 None:intergenic 45.0%
ATGGATACTCAGACTCAGGT+AGG + chr7.2:66516012-66516031 MS.gene041264:intron 45.0%
CACCAATGATTCAGCAGTGT+AGG - chr7.2:66515335-66515354 None:intergenic 45.0%
CAGCAAACCAAAACGGTCAA+GGG + chr7.2:66515468-66515487 MS.gene041264:CDS 45.0%
CAGTCAAGGGGATATCGAAT+AGG - chr7.2:66516205-66516224 None:intergenic 45.0%
CATGACAGAAAAAGTCGGCA+GGG + chr7.2:66514892-66514911 MS.gene041264:intron 45.0%
CTATTCGATATCCCCTTGAC+TGG + chr7.2:66516203-66516222 MS.gene041264:intron 45.0%
CTTGGTCCGAGTCTATAAAC+CGG + chr7.2:66515761-66515780 MS.gene041264:intron 45.0%
GAAAGCAGCATTAAAGAGGC+AGG + chr7.2:66515388-66515407 MS.gene041264:CDS 45.0%
GACCAACTAAACATAGGCGA+GGG - chr7.2:66514589-66514608 None:intergenic 45.0%
GATAGAATTGGTAGAAGCGG+TGG + chr7.2:66515873-66515892 MS.gene041264:intron 45.0%
GCTTAGTGACAGATGATGCA+AGG - chr7.2:66514510-66514529 None:intergenic 45.0%
GGAGTTAATGCCATTACTGC+AGG + chr7.2:66515301-66515320 MS.gene041264:CDS 45.0%
GTATCCAGAATGGTACGAAG+TGG - chr7.2:66514847-66514866 None:intergenic 45.0%
GTGTCTGCAACTGTAACTGA+TGG - chr7.2:66515280-66515299 None:intergenic 45.0%
GTTTGTCTTCAGACGAGAGA+TGG - chr7.2:66516118-66516137 None:intergenic 45.0%
TACCAGTCCTACCATCAATG+AGG + chr7.2:66515582-66515601 MS.gene041264:CDS 45.0%
TATTCCCTAGAGACAGACGA+CGG + chr7.2:66515154-66515173 MS.gene041264:intron 45.0%
TCAGTTACAGTTGCAGACAC+CGG + chr7.2:66515280-66515299 MS.gene041264:CDS 45.0%
TCATCTGTCACTAAGCGATG+AGG + chr7.2:66514514-66514533 MS.gene041264:CDS 45.0%
TCTCCATCATCATGTAGGAG+CGG - chr7.2:66514634-66514653 None:intergenic 45.0%
TGAAAACCCCTAATGAGCCA+AGG - chr7.2:66516161-66516180 None:intergenic 45.0%
TTGGTCGTTTCCACACAAAC+AGG + chr7.2:66514603-66514622 MS.gene041264:CDS 45.0%
! CAACACCCGGATTTTCATGT+AGG - chr7.2:66514984-66515003 None:intergenic 45.0%
! CATGTAGGATCTGAGGTAGT+TGG - chr7.2:66514969-66514988 None:intergenic 45.0%
! GAGATTCTTGGGACTTGCTT+CGG + chr7.2:66516039-66516058 MS.gene041264:intron 45.0%
! GCAGCCAAGCATTGTTAAAC+CGG - chr7.2:66516427-66516446 None:intergenic 45.0%
! TCATTGATGGTAGGACTGGT+AGG - chr7.2:66515583-66515602 None:intergenic 45.0%
! TCTGATTCCCTTGACCGTTT+TGG - chr7.2:66515478-66515497 None:intergenic 45.0%
! TGCTACTTCTGTGGATGTCT+AGG + chr7.2:66515103-66515122 MS.gene041264:intron 45.0%
!! GCACTACCATTGATGATGCA+AGG + chr7.2:66515551-66515570 MS.gene041264:CDS 45.0%
!! GCCTCTTTAATGCTGCTTTC+CGG - chr7.2:66515388-66515407 None:intergenic 45.0%
!!! CATTTTTTATCGGCAGAGCC+TGG + chr7.2:66515652-66515671 MS.gene041264:intron 45.0%
AAACCCCTAATGAGCCAAGG+AGG - chr7.2:66516158-66516177 None:intergenic 50.0%
AACCCCTAATGAGCCAAGGA+GGG - chr7.2:66516157-66516176 None:intergenic 50.0%
ACCTTTGACAGGGTTCCAGA+AGG - chr7.2:66514666-66514685 None:intergenic 50.0%
ACTACCGTCGTCTGTCTCTA+GGG - chr7.2:66515161-66515180 None:intergenic 50.0%
ACTGTCGTGTGCCTCATTGA+TGG - chr7.2:66515596-66515615 None:intergenic 50.0%
AGACGACGGTAGTCGTTTGT+GGG + chr7.2:66515168-66515187 MS.gene041264:intron 50.0%
AGGTAGGCGCAGAGATTCTT+GGG + chr7.2:66516028-66516047 MS.gene041264:intron 50.0%
ATGCTCCCATGACTGAGTTC+CGG - chr7.2:66515243-66515262 None:intergenic 50.0%
CAGAATGGTACGAAGTGGAG+AGG - chr7.2:66514842-66514861 None:intergenic 50.0%
CCAGCAAACCAAAACGGTCA+AGG + chr7.2:66515467-66515486 MS.gene041264:CDS 50.0%
CGACCAACTAAACATAGGCG+AGG - chr7.2:66514590-66514609 None:intergenic 50.0%
CGGACCAAGTCTTTCATGGT+AGG - chr7.2:66515750-66515769 None:intergenic 50.0%
CTCTCCACTTCGTACCATTC+TGG + chr7.2:66514840-66514859 MS.gene041264:intron 50.0%
CTCTCGTCTGAAGACAAACG+AGG + chr7.2:66516119-66516138 MS.gene041264:intron 50.0%
CTGCCAAGAGGATGTGATCA+TGG - chr7.2:66516294-66516313 None:intergenic 50.0%
CTGTCATGTATCCTGTTGGG+AGG - chr7.2:66514880-66514899 None:intergenic 50.0%
GACCCTCGCCTATGTTTAGT+TGG + chr7.2:66514584-66514603 MS.gene041264:CDS 50.0%
GACGATCCCTGGTGCATAAT+AGG + chr7.2:66516080-66516099 MS.gene041264:intron 50.0%
GACTCGGACCAAGTCTTTCA+TGG - chr7.2:66515754-66515773 None:intergenic 50.0%
GAGCCATGATCACATCCTCT+TGG + chr7.2:66516288-66516307 MS.gene041264:intron 50.0%
GCAATCAATCAGACGATCCC+TGG + chr7.2:66516069-66516088 MS.gene041264:intron 50.0%
GCCGGAAAGCAGCATTAAAG+AGG + chr7.2:66515384-66515403 MS.gene041264:CDS 50.0%
TAAACATAGGCGAGGGTCAC+TGG - chr7.2:66514582-66514601 None:intergenic 50.0%
TACGAAGTGGAGAGGGACTT+GGG - chr7.2:66514834-66514853 None:intergenic 50.0%
TCGTCTGAAGACAAACGAGG+AGG + chr7.2:66516122-66516141 MS.gene041264:intron 50.0%
TGCAACTTGATCCCATACCG+TGG + chr7.2:66516381-66516400 MS.gene041264:intron 50.0%
TGTATCCTGTTGGGAGGTCA+TGG - chr7.2:66514874-66514893 None:intergenic 50.0%
TGTCTTTCAACTCGCAGCTC+AGG - chr7.2:66515196-66515215 None:intergenic 50.0%
TTATATTCATTGCGCCCGGC+AGG - chr7.2:66515686-66515705 None:intergenic 50.0%
TTGTCACGTCGATCAACACC+CGG - chr7.2:66514997-66515016 None:intergenic 50.0%
! AATGGTAGTGCTTGCTGACG+TGG - chr7.2:66515542-66515561 None:intergenic 50.0%
! ATGGTAGTGCTTGCTGACGT+GGG - chr7.2:66515541-66515560 None:intergenic 50.0%
! ATGTGATCATGGCTCTGTCG+AGG - chr7.2:66516283-66516302 None:intergenic 50.0%
! GTATGGGATCAAGTTGCAGC+AGG - chr7.2:66516379-66516398 None:intergenic 50.0%
! TCGTGTGCCTCATTGATGGT+AGG - chr7.2:66515592-66515611 None:intergenic 50.0%
! TGCCTCATTGATGGTAGGAC+TGG - chr7.2:66515587-66515606 None:intergenic 50.0%
!! ACCTTCTGGAACCCTGTCAA+AGG + chr7.2:66514662-66514681 MS.gene041264:CDS 50.0%
!! GCGTTGATGACATGTCATCC+TGG + chr7.2:66516528-66516547 MS.gene041264:intron 50.0%
!!! CCTTGACCGTTTTGGTTTGC+TGG - chr7.2:66515470-66515489 None:intergenic 50.0%
!!! GGTTTTGGACCCTGCAGTAA+TGG - chr7.2:66515314-66515333 None:intergenic 50.0%
AAGTGGAGAGGGACTTGGGT+GGG - chr7.2:66514830-66514849 None:intergenic 55.0%
ATCGCCCTCCTTGGCTCATT+AGG + chr7.2:66516150-66516169 MS.gene041264:intron 55.0%
CAAGTCCATGACCTCCCAAC+AGG + chr7.2:66514866-66514885 MS.gene041264:intron 55.0%
CAGACGACGGTAGTCGTTTG+TGG + chr7.2:66515167-66515186 MS.gene041264:intron 55.0%
CAGGTAGGCGCAGAGATTCT+TGG + chr7.2:66516027-66516046 MS.gene041264:intron 55.0%
CGGATACATCAGCTGCCAAG+AGG - chr7.2:66516306-66516325 None:intergenic 55.0%
GAACTGCCTTAACGTTCCCC+AGG - chr7.2:66515728-66515747 None:intergenic 55.0%
GACGACGGTAGTCGTTTGTG+GGG + chr7.2:66515169-66515188 MS.gene041264:intron 55.0%
GACTACCGTCGTCTGTCTCT+AGG - chr7.2:66515162-66515181 None:intergenic 55.0%
GTACGAAGTGGAGAGGGACT+TGG - chr7.2:66514835-66514854 None:intergenic 55.0%
GTCAGCCCCATCGATGTTTC+CGG + chr7.2:66516405-66516424 MS.gene041264:intron 55.0%
TCGCCCTCCTTGGCTCATTA+GGG + chr7.2:66516151-66516170 MS.gene041264:intron 55.0%
TGGTAGAAGCGGTGGCATTG+CGG + chr7.2:66515881-66515900 MS.gene041264:intron 55.0%
! GGATCAAGTTGCAGCAGGAG+TGG - chr7.2:66516374-66516393 None:intergenic 55.0%
!! AGCGGATTGGCCTGTTTGTG+TGG - chr7.2:66514616-66514635 None:intergenic 55.0%
AGTGGAGAGGGACTTGGGTG+GGG - chr7.2:66514829-66514848 None:intergenic 60.0%
ATCTTGGAACTGCCGAGGCC+TGG + chr7.2:66515707-66515726 MS.gene041264:intron 60.0%
ATGAGCCAAGGAGGGCGATC+AGG - chr7.2:66516149-66516168 None:intergenic 60.0%
CCTTAACGTTCCCCAGGCCT+CGG - chr7.2:66515722-66515741 None:intergenic 60.0%
CGACAGTACATCAGCCCCCA+TGG + chr7.2:66515609-66515628 MS.gene041264:intron 60.0%
CGCCCTCCTTGGCTCATTAG+GGG + chr7.2:66516152-66516171 MS.gene041264:intron 60.0%
GAAGTGGAGAGGGACTTGGG+TGG - chr7.2:66514831-66514850 None:intergenic 60.0%
GCGAGGGTCACTGGACTGTT+GGG - chr7.2:66514573-66514592 None:intergenic 60.0%
GCGGTGGCATTGCGGTTCTT+TGG + chr7.2:66515889-66515908 MS.gene041264:intron 60.0%
GCTCGCCGGAACTCAGTCAT+GGG + chr7.2:66515235-66515254 MS.gene041264:CDS 60.0%
GTTGCAGCAGGAGTGGCGTA+TGG - chr7.2:66516367-66516386 None:intergenic 60.0%
TCTTGGAACTGCCGAGGCCT+GGG + chr7.2:66515708-66515727 MS.gene041264:intron 60.0%
TGAGCCAAGGAGGGCGATCA+GGG - chr7.2:66516148-66516167 None:intergenic 60.0%
TGCTCGCCGGAACTCAGTCA+TGG + chr7.2:66515234-66515253 MS.gene041264:CDS 60.0%
! GTCGAGGCGCGCTTCTTTTG+AGG - chr7.2:66516267-66516286 None:intergenic 60.0%
! GTGCTTGCTGACGTGGGAGA+AGG - chr7.2:66515535-66515554 None:intergenic 60.0%
ATGGGGCTGACGCCACGGTA+TGG - chr7.2:66516396-66516415 None:intergenic 65.0%
CATCGATGGGGCTGACGCCA+CGG - chr7.2:66516401-66516420 None:intergenic 65.0%
CCGAGGCCTGGGGAACGTTA+AGG + chr7.2:66515719-66515738 MS.gene041264:intron 65.0%
CTTGGAACTGCCGAGGCCTG+GGG + chr7.2:66515709-66515728 MS.gene041264:intron 65.0%
GGCGAGGGTCACTGGACTGT+TGG - chr7.2:66514574-66514593 None:intergenic 65.0%
GTGGAGAGGGACTTGGGTGG+GGG - chr7.2:66514828-66514847 None:intergenic 65.0%
TGGGGCTGACGCCACGGTAT+GGG - chr7.2:66516395-66516414 None:intergenic 65.0%
ACACTGGCGGAGCTGCTCGC+CGG + chr7.2:66515221-66515240 MS.gene041264:CDS 70.0%
ATCGGCAGAGCCTGGCTCCC+AGG + chr7.2:66515660-66515679 MS.gene041264:intron 70.0%
GAGGCCCTGATCGCCCTCCT+TGG + chr7.2:66516141-66516160 MS.gene041264:intron 70.0%
TCATTGCGCCCGGCAGGCCT+GGG - chr7.2:66515680-66515699 None:intergenic 70.0%
TGGGTGGGGGAGCCGTTCTC+TGG - chr7.2:66514815-66514834 None:intergenic 70.0%
TTCATTGCGCCCGGCAGGCC+TGG - chr7.2:66515681-66515700 None:intergenic 70.0%
AGCCTGGCTCCCAGGCCTGC+CGG + chr7.2:66515668-66515687 MS.gene041264:intron 75.0%
! GCCTGGCTCCCAGGCCTGCC+GGG + chr7.2:66515669-66515688 MS.gene041264:intron 80.0%
! GCCCGGCAGGCCTGGGAGCC+AGG - chr7.2:66515673-66515692 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 66514500 66516653 66514500 ID=MS.gene041264
chr7.2 mRNA 66514500 66516653 66514500 ID=MS.gene041264.t1;Parent=MS.gene041264
chr7.2 exon 66514500 66514774 66514500 ID=MS.gene041264.t1.exon1;Parent=MS.gene041264.t1
chr7.2 CDS 66514500 66514774 66514500 ID=cds.MS.gene041264.t1;Parent=MS.gene041264.t1
chr7.2 exon 66515180 66515613 66515180 ID=MS.gene041264.t1.exon2;Parent=MS.gene041264.t1
chr7.2 CDS 66515180 66515613 66515180 ID=cds.MS.gene041264.t1;Parent=MS.gene041264.t1
chr7.2 exon 66516598 66516653 66516598 ID=MS.gene041264.t1.exon3;Parent=MS.gene041264.t1
chr7.2 CDS 66516598 66516653 66516598 ID=cds.MS.gene041264.t1;Parent=MS.gene041264.t1
Gene Sequence

>MS.gene041264

ATGGCTTCCTTGCATCATCTGTCACTAAGCGATGAGGACGATGAACTCGAAATCCAGATTGATGAAATCTCCCAACAGTCCAGTGACCCTCGCCTATGTTTAGTTGGTCGTTTCCACACAAACAGGCCAATCCGCTCCTACATGATGATGGAGAAGATTGAAACCTTCTGGAACCCTGTCAAAGGTATCAAAATTCACGAAATCGAGCCAGGCCTATACACTTTCCAATTCAACCATCACCTTGATTTTCAAAGAATATTGAAGAAGGGTCCATGGTACTTTGACAATCACCTCTTGGTTCTTGATATCATTCCAGAGAACGGCTCCCCCACCCAAGTCCCTCTCCACTTCGTACCATTCTGGATACAAGTCCATGACCTCCCAACAGGATACATGACAGAAAAAGTCGGCAGGGATATCGCAAACTACATAGGTCAGTTCCTCGAATATGATGCAAAGAATAACTCCAACTACCTCAGATCCTACATGAAAATCCGGGTGTTGATCGACGTGACAAAGCCATTGAAGCGCCAGAAAAAGATTAAACGACATGGAGGGGATTCTTGTTTCATCAAATTCAAATACGAACGTCTAGGTAATTATTGCTACTTCTGTGGATGTCTAGGACATATTGAGGATTACTGTGAGAAACTATATTCCCTAGAGACAGACGACGGTAGTCGTTTGTGGGGTCCTGAGCTGCGAGTTGAAAGACAAAAGAACACTGGCGGAGCTGCTCGCCGGAACTCAGTCATGGGAGCATCGACAAGCATAACACCATCAGTTACAGTTGCAGACACCGGAGTTAATGCCATTACTGCAGGGTCCAAAACCTACACTGCTGAATCATTGGTGCGGCTGTTACAAAATCCAAAATTAATGCAGCCGGAAAGCAGCATTAAAGAGGCAGGAAAACTGAAACAGAATGCTGATATTCTGCAAGAAGAAAATCAAGAAACAATTATCACCAGCAAACCAAAACGGTCAAGGGAATCAGAAGATAATGCAGCAATAAACGCTGCAATTATTCAGCCTTCTCCCACGTCAGCAAGCACTACCATTGATGATGCAAGGAATAGTCCTACCAGTCCTACCATCAATGAGGCACACGACAGTACATCAGCCCCCATGGAGATTTCACATACATCAATTCATTTTTTATCGGCAGAGCCTGGCTCCCAGGCCTGCCGGGCGCAATGAATATAATATCTTGGAACTGCCGAGGCCTGGGGAACGTTAAGGCAGTTCCTACCATGAAAGACTTGGTCCGAGTCTATAAACCGGACATTGTTATCCTTATCGAAACTTTAGTGAATGATAATAAAATTTCTGAATTATGCTACAACATTGGATTTGAAAATCATTTTACTGTTGATAGAATTGGTAGAAGCGGTGGCATTGCGGTTCTTTGGCGCAATTCTGTCGATTGCACGTTAATTAATTTCTCCCAAAACTTTGTCAACCTTAAAGTCAAAGATCAGATTAACGGCAACTGGAGGTTAACCGCTTTTTATGGATACTCAGACTCAGGTAGGCGCAGAGATTCTTGGGACTTGCTTCGGTCTCTAAGCAATCAATCAGACGATCCCTGGTGCATAATAGGAGACTTTAATGACCATCTCTCGTCTGAAGACAAACGAGGAGGCCCTGATCGCCCTCCTTGGCTCATTAGGGGTTTTCAAAATGCTGTGAATGACTGTAACCTATTCGATATCCCCTTGACTGGTTATCAATTCACATGGTTCAAAAGTATCGGCACTGAATCCTCAAAAGAAGCGCGCCTCGACAGAGCCATGATCACATCCTCTTGGCAGCTGATGTATCCGAATGCAACCTTGCAAACTCTTGTTGCTCCTATATCAGACCATACGCCACTCCTGCTGCAACTTGATCCCATACCGTGGCGTCAGCCCCATCGATGTTTCCGGTTTAACAATGCTTGGCTGCTTGAACCTGAACTGAATCACTTGGTCAAATTGAATTGGGCACATTACCCTCCTTCTGATGTTATAACGAAGCTCAAACATTGCGTTGATGACATGTCATCCTGGAGTAAAAATCTGATCCCAAATTTTCGGCAACTCATCAACAAACAAAGGTCAGCCATTGAAGCTTTTCGAAGCTCTTCTGCAGATGCAAGTGACCCGCAATTGA
Protein sequence

>MS.gene041264.t1

MASLHHLSLSDEDDELEIQIDEISQQSSDPRLCLVGRFHTNRPIRSYMMMEKIETFWNPVKGIKIHEIEPGLYTFQFNHHLDFQRILKKGPCRLWGPELRVERQKNTGGAARRNSVMGASTSITPSVTVADTGVNAITAGSKTYTAESLVRLLQNPKLMQPESSIKEAGKLKQNADILQEENQETIITSKPKRSRESEDNAAINAAIIQPSPTSASTTIDDARNSPTSPTINEAHDSQPLKLFEALLQMQVTRN