AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09517.t1 MTR_3g053920 53.846 247 49 2 1 247 1 182 3.53e-85 258
MS.gene09517.t1 MTR_2g061500 76.744 129 30 0 1 129 1 129 6.89e-69 211
MS.gene09517.t1 MTR_4g103530 74.257 101 25 1 55 155 1 100 1.63e-48 161
MS.gene09517.t1 MTR_6g033215 34.682 173 111 1 29 199 122 294 9.62e-34 125
MS.gene09517.t1 MTR_7g011710 63.529 85 31 0 55 139 1 85 4.98e-32 117
MS.gene09517.t1 MTR_0010s0370 34.286 175 111 2 7 177 3 177 1.22e-29 112
MS.gene09517.t1 MTR_0010s0350 34.286 175 111 2 7 177 3 177 1.22e-29 112
MS.gene09517.t1 MTR_1g036720 35.135 111 66 3 7 113 3 111 5.24e-12 62.4
MS.gene09517.t1 MTR_7g089990 35.185 108 66 2 106 209 86 193 2.80e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09517.t1 AT3G31430 27.174 184 124 5 36 215 134 311 4.56e-15 75.1
MS.gene09517.t1 AT2G17920 25.946 185 129 4 36 216 39 219 4.74e-15 74.7
MS.gene09517.t1 AT5G36228 27.273 198 135 5 36 227 39 233 4.19e-14 72.8
MS.gene09517.t1 AT3G57587 25.000 216 152 5 7 216 8 219 4.90e-13 69.3
MS.gene09517.t1 AT2G41590 24.762 210 150 4 11 216 14 219 1.90e-12 67.8
MS.gene09517.t1 AT2G13450 25.946 185 129 4 35 215 38 218 3.27e-12 67.0
MS.gene09517.t1 AT4G02000 27.481 131 91 2 88 216 11 139 1.38e-11 64.7
MS.gene09517.t1 AT3G47920 27.333 150 103 3 71 216 1 148 2.15e-11 63.9

Find 77 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGATTGGATTCGGATTT+TGG 0.148471 6.4:-25347781 None:intergenic
GGTGAATCTGGATGAATTTC+TGG 0.167657 6.4:-25347690 None:intergenic
CAGATGATGGTGTCCGTAAT+TGG 0.189817 6.4:+25346499 MS.gene09517:CDS
GCTCGCTGAAATAGGAAAAT+TGG 0.201535 6.4:-25345969 None:intergenic
CGTTGAATCACATAAAGTTT+TGG 0.262299 6.4:+25346178 MS.gene09517:CDS
AGACATACGCTCGCTGAAAT+AGG 0.263035 6.4:-25345977 None:intergenic
ATCTGTGGTTGATCGCTATT+TGG 0.268773 6.4:+25346038 MS.gene09517:CDS
GAATCAAGCTCGAACAATTC+AGG 0.274635 6.4:-25346477 None:intergenic
CCTATACCTTGACCGACTTT+TGG 0.279802 6.4:-25346237 None:intergenic
TGTCACAGGTGTGGAGTTAT+TGG 0.281274 6.4:+25346435 MS.gene09517:CDS
CCAAAAGTCGGTCAAGGTAT+AGG 0.308271 6.4:+25346237 MS.gene09517:CDS
AAACCGGTCACTCAACAAAT+TGG 0.313889 6.4:+25346534 MS.gene09517:CDS
TAGGTTTAACTTCTAGGTTT+AGG 0.320787 6.4:-25345888 None:intergenic
CTGTCTGATCGGTCGAGTTT+TGG 0.325888 6.4:+25345933 MS.gene09517:CDS
AACAATATCACCGCAAGAAT+TGG 0.348723 6.4:+25347802 MS.gene09517:CDS
AGATGATGGTGTCCGTAATT+GGG 0.354245 6.4:+25346500 MS.gene09517:CDS
TCTAACAAAAGTACCATTAC+TGG 0.354581 6.4:-25346379 None:intergenic
CAATGGGACAGTGACGTCTA+TGG 0.362240 6.4:-25346334 None:intergenic
CAATACAATGCATGATAATA+TGG 0.362887 6.4:-25346128 None:intergenic
TGCGGAACAATTGGTGAATC+TGG 0.363329 6.4:-25347702 None:intergenic
CAAGGTATAGGGAGTTTCTT+AGG 0.375903 6.4:+25346249 MS.gene09517:CDS
TGCGGTGATATTGTTGAGAT+TGG 0.378149 6.4:-25347794 None:intergenic
CAAAAGTCGGTCAAGGTATA+GGG 0.382596 6.4:+25346238 MS.gene09517:CDS
ACCATCAAGGTTGAGGGTTG+TGG 0.383838 6.4:-25345839 None:intergenic
CAGGTTCACGGATTGCCATT+TGG 0.385702 6.4:+25346204 MS.gene09517:CDS
GATATTGTTGAGATTGGATT+CGG 0.388995 6.4:-25347788 None:intergenic
TTTGGTTCAATTTCGCCATA+AGG 0.400769 6.4:+25346056 MS.gene09517:CDS
GAGTTTCTTAGGCACTCTTA+AGG 0.401851 6.4:+25346260 MS.gene09517:CDS
CACGGGCATCTTCGACCTTA+TGG 0.403235 6.4:-25346071 None:intergenic
CCCACAACCCTCAACCTTGA+TGG 0.410375 6.4:+25345838 MS.gene09517:CDS
GGCCACTTTGATTCGCATAT+AGG 0.410527 6.4:-25346313 None:intergenic
TGAGTGACCGGTTTGAGATA+TGG 0.441522 6.4:-25346525 None:intergenic
CCATCAAGGTTGAGGGTTGT+GGG 0.447309 6.4:-25345838 None:intergenic
TGTGGTTGTTTGCGATGAAA+TGG 0.451946 6.4:-25347661 None:intergenic
ATGTCTCGTGCTTGGAAGCC+AGG 0.453399 6.4:+25345994 MS.gene09517:CDS
ACCGCAAGAATTGGCATCTC+AGG 0.457956 6.4:+25347811 MS.gene09517:CDS
CAAATGGCAATCCGTGAACC+TGG 0.458963 6.4:-25346203 None:intergenic
TGATTCAACGGGACAAGATT+CGG 0.460494 6.4:-25346165 None:intergenic
AAAACTTTATGTGATTCAAC+GGG 0.470210 6.4:-25346176 None:intergenic
GTGATCGTAACCCTCTTGCC+TGG 0.472089 6.4:-25346012 None:intergenic
TTCGAGCTTGATTCAGATGA+TGG 0.484166 6.4:+25346486 MS.gene09517:CDS
GTTCCAATTTGTTGAGTGAC+CGG 0.489190 6.4:-25346537 None:intergenic
ATGATGAATTGCGGAACAAT+TGG 0.490462 6.4:-25347711 None:intergenic
AATAGGAAAATTGGATTTCG+CGG 0.497676 6.4:-25345960 None:intergenic
TGTGAGACCATCAAGGTTGA+GGG 0.503307 6.4:-25345845 None:intergenic
GATGATGGTGTCCGTAATTG+GGG 0.504296 6.4:+25346501 MS.gene09517:CDS
CAAAACTTTATGTGATTCAA+CGG 0.504821 6.4:-25346177 None:intergenic
GTCATTGACCACTGTCTGAT+CGG 0.507295 6.4:+25345922 MS.gene09517:CDS
ATCATGCATTGTATTGCTCC+TGG 0.509937 6.4:+25346135 MS.gene09517:CDS
ATGTGAGACCATCAAGGTTG+AGG 0.511824 6.4:-25345846 None:intergenic
AATCAAGCTCGAACAATTCA+GGG 0.514945 6.4:-25346476 None:intergenic
GCGAGCGTATGTCTCGTGCT+TGG 0.539091 6.4:+25345986 MS.gene09517:CDS
TTTGGCTGGATGAAACCAAA+TGG 0.543514 6.4:-25346219 None:intergenic
TGGCGTGTTCGTGCCAGTAA+TGG 0.544310 6.4:+25346366 MS.gene09517:CDS
ATGGACCTTCATCGAGGACA+CGG 0.544404 6.4:-25346089 None:intergenic
GATGCCCGTGTCCTCGATGA+AGG 0.548987 6.4:+25346084 MS.gene09517:CDS
ACAAGATTCGGTACAACACC+AGG 0.555835 6.4:-25346153 None:intergenic
ATGAATTTCTGGAAATATTG+TGG 0.573370 6.4:-25347679 None:intergenic
GTGTCCTCGATGAAGGTCCA+TGG 0.573839 6.4:+25346091 MS.gene09517:CDS
GGTCTCACATTGTCCGATGA+CGG 0.585431 6.4:+25345859 MS.gene09517:CDS
ACCTGAGATGCCAATTCTTG+CGG 0.587507 6.4:-25347812 None:intergenic
CTCCTATATGCGAATCAAAG+TGG 0.596373 6.4:+25346311 MS.gene09517:CDS
CGGACAATGTGAGACCATCA+AGG 0.598298 6.4:-25345852 None:intergenic
GGTTTCATCCAGCCAAAAGT+CGG 0.598864 6.4:+25346225 MS.gene09517:CDS
GTTTAGGATGAGACCGTCAT+CGG 0.610995 6.4:-25345872 None:intergenic
ATCCAGCCAAAAGTCGGTCA+AGG 0.612990 6.4:+25346231 MS.gene09517:CDS
TGGAAGTTATCATGAAGCCA+TGG 0.619258 6.4:-25346108 None:intergenic
TGCTTGGAAGCCAGGCAAGA+GGG 0.631655 6.4:+25346002 MS.gene09517:CDS
GTGCTTGGAAGCCAGGCAAG+AGG 0.641181 6.4:+25346001 MS.gene09517:CDS
TGGACCTTCATCGAGGACAC+GGG 0.644854 6.4:-25346088 None:intergenic
GGTTACGATCACTAAATCTG+TGG 0.668103 6.4:+25346023 MS.gene09517:CDS
AGGAGATGTGAATGGTGTTG+CGG 0.673222 6.4:-25346293 None:intergenic
TCGCATATAGGAGATGTGAA+TGG 0.681501 6.4:-25346301 None:intergenic
AAACTCGACCGATCAGACAG+TGG 0.728408 6.4:-25345930 None:intergenic
ACAGTGGTCAATGACAGCTG+CGG 0.741186 6.4:-25345914 None:intergenic
ATGATGGTGTCCGTAATTGG+GGG 0.743261 6.4:+25346502 MS.gene09517:CDS
GAAGCCATGGACCTTCATCG+AGG 0.747423 6.4:-25346095 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAATTAAAATTGGTAAA+CGG - chr6.4:25347174-25347193 None:intergenic 15.0%
!!! ATTGAAAGAAATTTTAACAT+AGG - chr6.4:25346752-25346771 None:intergenic 15.0%
!!! TTTTAAAATGGATTTTTAAC+AGG - chr6.4:25346990-25347009 None:intergenic 15.0%
!! AAAATTAGGGTATAACAATA+GGG - chr6.4:25346590-25346609 None:intergenic 20.0%
!! AACGATTAAAAATGACAAAA+CGG - chr6.4:25347134-25347153 None:intergenic 20.0%
!! TTTGACATAGAATTGAAATA+AGG - chr6.4:25346818-25346837 None:intergenic 20.0%
!!! AACACAATTTTAGGAAATTT+AGG - chr6.4:25347220-25347239 None:intergenic 20.0%
!!! AATTTCCTAAAATTGTGTTT+TGG + chr6.4:25347221-25347240 MS.gene09517:intron 20.0%
!!! ACAATTTTAGAACTTAAACA+GGG - chr6.4:25346922-25346941 None:intergenic 20.0%
!!! TATTTTTAGGTCCAAAATTA+GGG - chr6.4:25346603-25346622 None:intergenic 20.0%
! AAAACTTTATGTGATTCAAC+GGG - chr6.4:25346179-25346198 None:intergenic 25.0%
! AACTTCAAATACGAAAAACT+CGG + chr6.4:25346405-25346424 MS.gene09517:CDS 25.0%
! AAGTACCTCATTTAAGTAAT+TGG + chr6.4:25347097-25347116 MS.gene09517:intron 25.0%
! CAAAACTTTATGTGATTCAA+CGG - chr6.4:25346180-25346199 None:intergenic 25.0%
! CAAAATTAGGGTATAACAAT+AGG - chr6.4:25346591-25346610 None:intergenic 25.0%
! CAATACAATGCATGATAATA+TGG - chr6.4:25346131-25346150 None:intergenic 25.0%
!! ACTCGATTTTTGATGAATTT+TGG - chr6.4:25347744-25347763 None:intergenic 25.0%
!! AGAATTGGACAAGTATTTTT+AGG - chr6.4:25346616-25346635 None:intergenic 25.0%
!! ATGAATTTCTGGAAATATTG+TGG - chr6.4:25347682-25347701 None:intergenic 25.0%
!! CTATTGTTATACCCTAATTT+TGG + chr6.4:25346589-25346608 MS.gene09517:intron 25.0%
!! GTTGAATCACATAAAGTTTT+GGG + chr6.4:25346179-25346198 MS.gene09517:CDS 25.0%
!! TTGTTGTTGATTATGAGAAT+TGG - chr6.4:25347613-25347632 None:intergenic 25.0%
!!! ACTCAGATGCAATTTTAAAA+TGG - chr6.4:25347002-25347021 None:intergenic 25.0%
!!! GACAATTTTAGAACTTAAAC+AGG - chr6.4:25346923-25346942 None:intergenic 25.0%
!!! GTATTTTTAGGTCCAAAATT+AGG - chr6.4:25346604-25346623 None:intergenic 25.0%
AAATGCACGAAAAGAAGAAT+TGG - chr6.4:25346631-25346650 None:intergenic 30.0%
AATAGGAAAATTGGATTTCG+CGG - chr6.4:25345963-25345982 None:intergenic 30.0%
ACGAACCAATTACTTAAATG+AGG - chr6.4:25347105-25347124 None:intergenic 30.0%
ACTTGCAAAATTCACATAGA+AGG + chr6.4:25346711-25346730 MS.gene09517:intron 30.0%
ATGAGTTGTACAACAATGAA+TGG - chr6.4:25347433-25347452 None:intergenic 30.0%
CTTGCAAAATTCACATAGAA+GGG + chr6.4:25346712-25346731 MS.gene09517:intron 30.0%
GATATTGTTGAGATTGGATT+CGG - chr6.4:25347791-25347810 None:intergenic 30.0%
GTGATATTTGAACCAGTAAT+GGG - chr6.4:25347364-25347383 None:intergenic 30.0%
TGTGATATTTGAACCAGTAA+TGG - chr6.4:25347365-25347384 None:intergenic 30.0%
! ATTGTTTGAGTCTTTTCAAC+TGG + chr6.4:25346781-25346800 MS.gene09517:intron 30.0%
! CGTTGAATCACATAAAGTTT+TGG + chr6.4:25346178-25346197 MS.gene09517:CDS 30.0%
! TAGGTTTAACTTCTAGGTTT+AGG - chr6.4:25345891-25345910 None:intergenic 30.0%
! TCCATTTCAAAAAGTGCATT+TGG + chr6.4:25346839-25346858 MS.gene09517:intron 30.0%
! TTGTTGATTATGAGAATTGG+TGG - chr6.4:25347610-25347629 None:intergenic 30.0%
!! TCCAAATGCACTTTTTGAAA+TGG - chr6.4:25346843-25346862 None:intergenic 30.0%
!! TCTAACAAAAGTACCATTAC+TGG - chr6.4:25346382-25346401 None:intergenic 30.0%
AAAAGACTAAAATGCCCCTA+GGG - chr6.4:25346887-25346906 None:intergenic 35.0%
AACAATATCACCGCAAGAAT+TGG + chr6.4:25347802-25347821 MS.gene09517:CDS 35.0%
AATCAAGCTCGAACAATTCA+GGG - chr6.4:25346479-25346498 None:intergenic 35.0%
ATGATGAATTGCGGAACAAT+TGG - chr6.4:25347714-25347733 None:intergenic 35.0%
TGCAAAGAGGACAAAATACA+AGG - chr6.4:25347273-25347292 None:intergenic 35.0%
TGGACTGCAATTAACAATAC+TGG - chr6.4:25347248-25347267 None:intergenic 35.0%
TGTTACATTTACAGTCCATG+TGG + chr6.4:25347335-25347354 MS.gene09517:intron 35.0%
! AAGTGTTTGGATTGGATTTC+TGG - chr6.4:25347570-25347589 None:intergenic 35.0%
! ATTTTGTCCTCTTTGCAGAA+AGG + chr6.4:25347276-25347295 MS.gene09517:intron 35.0%
! CTGGACCAAAACACAATTTT+AGG - chr6.4:25347229-25347248 None:intergenic 35.0%
! GATTCGGATTTTGGATGTTA+GGG - chr6.4:25347775-25347794 None:intergenic 35.0%
! GGTCATTTCACACGAAATTT+TGG + chr6.4:25347056-25347075 MS.gene09517:intron 35.0%
! TTGAGATTGGATTCGGATTT+TGG - chr6.4:25347784-25347803 None:intergenic 35.0%
! TTTGGTTCAATTTCGCCATA+AGG + chr6.4:25346056-25346075 MS.gene09517:CDS 35.0%
! TTTTGTCCTCTTTGCAGAAA+GGG + chr6.4:25347277-25347296 MS.gene09517:intron 35.0%
!! TGAGAATTGGTGGTGAATTA+TGG - chr6.4:25347600-25347619 None:intergenic 35.0%
!! TTTAGTCGTTTTAGTCTCCA+GGG + chr6.4:25347026-25347045 MS.gene09517:intron 35.0%
!! TTTTAGTCGTTTTAGTCTCC+AGG + chr6.4:25347025-25347044 MS.gene09517:intron 35.0%
!!! CGGTTTTAGGTTTAACTTCT+AGG - chr6.4:25345897-25345916 None:intergenic 35.0%
!!! GGTTTTGAGATGATGAATTG+CGG - chr6.4:25347723-25347742 None:intergenic 35.0%
AAACCGGTCACTCAACAAAT+TGG + chr6.4:25346534-25346553 MS.gene09517:CDS 40.0%
AAAGACTAAAATGCCCCTAG+GGG - chr6.4:25346886-25346905 None:intergenic 40.0%
ACTCTAACTGCTTTGACCTA+TGG - chr6.4:25347304-25347323 None:intergenic 40.0%
AGAGGACAAAATACAAGGAC+TGG - chr6.4:25347268-25347287 None:intergenic 40.0%
ATCATGCATTGTATTGCTCC+TGG + chr6.4:25346135-25346154 MS.gene09517:CDS 40.0%
ATCTGTGGTTGATCGCTATT+TGG + chr6.4:25346038-25346057 MS.gene09517:CDS 40.0%
CAAAAGTCGGTCAAGGTATA+GGG + chr6.4:25346238-25346257 MS.gene09517:CDS 40.0%
CAAGGTATAGGGAGTTTCTT+AGG + chr6.4:25346249-25346268 MS.gene09517:CDS 40.0%
CTCCTATATGCGAATCAAAG+TGG + chr6.4:25346311-25346330 MS.gene09517:CDS 40.0%
CTCTAACTGCTTTGACCTAT+GGG - chr6.4:25347303-25347322 None:intergenic 40.0%
CTGTCCCATTGAAAAAAGAG+TGG + chr6.4:25346346-25346365 MS.gene09517:CDS 40.0%
GAAAAGACTAAAATGCCCCT+AGG - chr6.4:25346888-25346907 None:intergenic 40.0%
GAATCAAGCTCGAACAATTC+AGG - chr6.4:25346480-25346499 None:intergenic 40.0%
GAGTTTCTTAGGCACTCTTA+AGG + chr6.4:25346260-25346279 MS.gene09517:CDS 40.0%
GCTCGCTGAAATAGGAAAAT+TGG - chr6.4:25345972-25345991 None:intergenic 40.0%
GGTGAATCTGGATGAATTTC+TGG - chr6.4:25347693-25347712 None:intergenic 40.0%
GGTTACGATCACTAAATCTG+TGG + chr6.4:25346023-25346042 MS.gene09517:CDS 40.0%
TAGGTCAAAGCAGTTAGAGT+TGG + chr6.4:25347304-25347323 MS.gene09517:intron 40.0%
TCGCATATAGGAGATGTGAA+TGG - chr6.4:25346304-25346323 None:intergenic 40.0%
TGATTCAACGGGACAAGATT+CGG - chr6.4:25346168-25346187 None:intergenic 40.0%
TGCGGTGATATTGTTGAGAT+TGG - chr6.4:25347797-25347816 None:intergenic 40.0%
TGGAAGTTATCATGAAGCCA+TGG - chr6.4:25346111-25346130 None:intergenic 40.0%
TGTGGTTGTTTGCGATGAAA+TGG - chr6.4:25347664-25347683 None:intergenic 40.0%
TTCGAGCTTGATTCAGATGA+TGG + chr6.4:25346486-25346505 MS.gene09517:CDS 40.0%
TTTGGCTGGATGAAACCAAA+TGG - chr6.4:25346222-25346241 None:intergenic 40.0%
! AGATGATGGTGTCCGTAATT+GGG + chr6.4:25346500-25346519 MS.gene09517:CDS 40.0%
! GGATTCGGATTTTGGATGTT+AGG - chr6.4:25347776-25347795 None:intergenic 40.0%
!! GTTCCAATTTGTTGAGTGAC+CGG - chr6.4:25346540-25346559 None:intergenic 40.0%
!! TTAGTCGTTTTAGTCTCCAG+GGG + chr6.4:25347027-25347046 MS.gene09517:intron 40.0%
!! TTATGGCAGTGAGAAGTGTT+TGG - chr6.4:25347583-25347602 None:intergenic 40.0%
!!! ACACGCCACTCTTTTTTCAA+TGG - chr6.4:25346354-25346373 None:intergenic 40.0%
!!! CACGCCACTCTTTTTTCAAT+GGG - chr6.4:25346353-25346372 None:intergenic 40.0%
!!! TCACATAAAGTTTTGGGTCC+AGG + chr6.4:25346185-25346204 MS.gene09517:CDS 40.0%
ACCTGAGATGCCAATTCTTG+CGG - chr6.4:25347815-25347834 None:intergenic 45.0%
AGACATACGCTCGCTGAAAT+AGG - chr6.4:25345980-25345999 None:intergenic 45.0%
ATGAATGGATCCGTGTGACT+TGG - chr6.4:25347418-25347437 None:intergenic 45.0%
ATGTGAGACCATCAAGGTTG+AGG - chr6.4:25345849-25345868 None:intergenic 45.0%
CCAAAAGTCGGTCAAGGTAT+AGG + chr6.4:25346237-25346256 MS.gene09517:CDS 45.0%
GCTATAAATAGCCCAGTCAC+AGG + chr6.4:25347392-25347411 MS.gene09517:intron 45.0%
GGTTTCATCCAGCCAAAAGT+CGG + chr6.4:25346225-25346244 MS.gene09517:CDS 45.0%
GTCATTGACCACTGTCTGAT+CGG + chr6.4:25345922-25345941 MS.gene09517:CDS 45.0%
TGAAATGACCAAAATGCCCC+TGG - chr6.4:25347046-25347065 None:intergenic 45.0%
TGAGATATGGCCCCCAATTA+CGG - chr6.4:25346515-25346534 None:intergenic 45.0%
TGCGGAACAATTGGTGAATC+TGG - chr6.4:25347705-25347724 None:intergenic 45.0%
TGTCACAGGTGTGGAGTTAT+TGG + chr6.4:25346435-25346454 MS.gene09517:CDS 45.0%
TGTGAGACCATCAAGGTTGA+GGG - chr6.4:25345848-25345867 None:intergenic 45.0%
TTGAGTCAGTTTAGCCCCTA+GGG + chr6.4:25346869-25346888 MS.gene09517:intron 45.0%
TTTGAGTCAGTTTAGCCCCT+AGG + chr6.4:25346868-25346887 MS.gene09517:intron 45.0%
! ACAAGATTCGGTACAACACC+AGG - chr6.4:25346156-25346175 None:intergenic 45.0%
! AGGAGATGTGAATGGTGTTG+CGG - chr6.4:25346296-25346315 None:intergenic 45.0%
! ATGATGGTGTCCGTAATTGG+GGG + chr6.4:25346502-25346521 MS.gene09517:CDS 45.0%
! CAGATGATGGTGTCCGTAAT+TGG + chr6.4:25346499-25346518 MS.gene09517:CDS 45.0%
! GATGATGGTGTCCGTAATTG+GGG + chr6.4:25346501-25346520 MS.gene09517:CDS 45.0%
! GGCCACTTTGATTCGCATAT+AGG - chr6.4:25346316-25346335 None:intergenic 45.0%
! GTTTAGGATGAGACCGTCAT+CGG - chr6.4:25345875-25345894 None:intergenic 45.0%
! TCAATGACAGCTGCGGTTTT+AGG - chr6.4:25345910-25345929 None:intergenic 45.0%
!! CACGAAATTTTGGCAGAGAG+AGG + chr6.4:25347066-25347085 MS.gene09517:intron 45.0%
!! CCTATACCTTGACCGACTTT+TGG - chr6.4:25346240-25346259 None:intergenic 45.0%
!! GCAGTGAGAAGTGTTTGGAT+TGG - chr6.4:25347578-25347597 None:intergenic 45.0%
!! TGAGTGACCGGTTTGAGATA+TGG - chr6.4:25346528-25346547 None:intergenic 45.0%
!!! AACTCGGTGTCTTTTGTCAC+AGG + chr6.4:25346421-25346440 MS.gene09517:CDS 45.0%
!!! AAGTTTTGGGTCCAGGTTCA+CGG + chr6.4:25346192-25346211 MS.gene09517:CDS 45.0%
!!! TTTAGTCTCCAGGGGCATTT+TGG + chr6.4:25347035-25347054 MS.gene09517:intron 45.0%
!! AAAATAAAATGAATTAAAAT+TGG - chr6.4:25347181-25347200 None:intergenic 5.0%
AAACTCGACCGATCAGACAG+TGG - chr6.4:25345933-25345952 None:intergenic 50.0%
ACAGTGGTCAATGACAGCTG+CGG - chr6.4:25345917-25345936 None:intergenic 50.0%
ACCATCAAGGTTGAGGGTTG+TGG - chr6.4:25345842-25345861 None:intergenic 50.0%
ACCGCAAGAATTGGCATCTC+AGG + chr6.4:25347811-25347830 MS.gene09517:CDS 50.0%
ATCCAGCCAAAAGTCGGTCA+AGG + chr6.4:25346231-25346250 MS.gene09517:CDS 50.0%
ATGGACCTTCATCGAGGACA+CGG - chr6.4:25346092-25346111 None:intergenic 50.0%
CAAATGGCAATCCGTGAACC+TGG - chr6.4:25346206-25346225 None:intergenic 50.0%
CAATGGGACAGTGACGTCTA+TGG - chr6.4:25346337-25346356 None:intergenic 50.0%
CCATCAAGGTTGAGGGTTGT+GGG - chr6.4:25345841-25345860 None:intergenic 50.0%
CGGACAATGTGAGACCATCA+AGG - chr6.4:25345855-25345874 None:intergenic 50.0%
GAACTGCTGCTACCAATCGT+TGG + chr6.4:25346556-25346575 MS.gene09517:intron 50.0%
GGTCTCACATTGTCCGATGA+CGG + chr6.4:25345859-25345878 MS.gene09517:CDS 50.0%
TATGGGCCCTTTCTGCAAAG+AGG - chr6.4:25347286-25347305 None:intergenic 50.0%
TCTTTGCAGAAAGGGCCCAT+AGG + chr6.4:25347285-25347304 MS.gene09517:intron 50.0%
TGAGTCAGTTTAGCCCCTAG+GGG + chr6.4:25346870-25346889 MS.gene09517:intron 50.0%
TTGGGGGCCATATCTCAAAC+CGG + chr6.4:25346518-25346537 MS.gene09517:CDS 50.0%
! CAGGTTCACGGATTGCCATT+TGG + chr6.4:25346204-25346223 MS.gene09517:CDS 50.0%
! CTGTCTGATCGGTCGAGTTT+TGG + chr6.4:25345933-25345952 MS.gene09517:CDS 50.0%
! GGTGTCTTTTGTCACAGGTG+TGG + chr6.4:25346426-25346445 MS.gene09517:CDS 50.0%
!! TACCTTGACCGACTTTTGGC+TGG - chr6.4:25346236-25346255 None:intergenic 50.0%
!! TGCTACCAATCGTTGGCTTC+AGG + chr6.4:25346563-25346582 MS.gene09517:intron 50.0%
AGGGTCCTGAAGCCAACGAT+TGG - chr6.4:25346571-25346590 None:intergenic 55.0%
ATGTCTCGTGCTTGGAAGCC+AGG + chr6.4:25345994-25346013 MS.gene09517:CDS 55.0%
CACGGGCATCTTCGACCTTA+TGG - chr6.4:25346074-25346093 None:intergenic 55.0%
CAGTCACAGGCCAAGTCACA+CGG + chr6.4:25347405-25347424 MS.gene09517:intron 55.0%
CCCACAACCCTCAACCTTGA+TGG + chr6.4:25345838-25345857 MS.gene09517:CDS 55.0%
GAAGCCATGGACCTTCATCG+AGG - chr6.4:25346098-25346117 None:intergenic 55.0%
GTGATCGTAACCCTCTTGCC+TGG - chr6.4:25346015-25346034 None:intergenic 55.0%
GTGTCCTCGATGAAGGTCCA+TGG + chr6.4:25346091-25346110 MS.gene09517:CDS 55.0%
TCCATGTGGCAGCCCATTAC+TGG + chr6.4:25347349-25347368 MS.gene09517:intron 55.0%
TGCTTGGAAGCCAGGCAAGA+GGG + chr6.4:25346002-25346021 MS.gene09517:CDS 55.0%
TGGACCTTCATCGAGGACAC+GGG - chr6.4:25346091-25346110 None:intergenic 55.0%
TGGCGTGTTCGTGCCAGTAA+TGG + chr6.4:25346366-25346385 MS.gene09517:CDS 55.0%
! GTGTGACTTGGCCTGTGACT+GGG - chr6.4:25347406-25347425 None:intergenic 55.0%
!! ACCAGTAATGGGCTGCCACA+TGG - chr6.4:25347353-25347372 None:intergenic 55.0%
GATGCCCGTGTCCTCGATGA+AGG + chr6.4:25346084-25346103 MS.gene09517:CDS 60.0%
GCGAGCGTATGTCTCGTGCT+TGG + chr6.4:25345986-25346005 MS.gene09517:CDS 60.0%
GTGCTTGGAAGCCAGGCAAG+AGG + chr6.4:25346001-25346020 MS.gene09517:CDS 60.0%
! CGTGTGACTTGGCCTGTGAC+TGG - chr6.4:25347407-25347426 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 25345832 25347859 25345832 ID=MS.gene09517
chr6.4 mRNA 25345832 25347859 25345832 ID=MS.gene09517.t1;Parent=MS.gene09517
chr6.4 exon 25345832 25346573 25345832 ID=MS.gene09517.t1.exon1;Parent=MS.gene09517.t1
chr6.4 CDS 25345832 25346573 25345832 ID=cds.MS.gene09517.t1;Parent=MS.gene09517.t1
chr6.4 exon 25347636 25347859 25347636 ID=MS.gene09517.t1.exon2;Parent=MS.gene09517.t1
chr6.4 CDS 25347636 25347859 25347636 ID=cds.MS.gene09517.t1;Parent=MS.gene09517.t1
Gene Sequence

>MS.gene09517

ATGGATCCCACAACCCTCAACCTTGATGGTCTCACATTGTCCGATGACGGTCTCATCCTAAACCTAGAAGTTAAACCTAAAACCGCAGCTGTCATTGACCACTGTCTGATCGGTCGAGTTTTGGCTAACCGCGAAATCCAATTTTCCTATTTCAGCGAGCGTATGTCTCGTGCTTGGAAGCCAGGCAAGAGGGTTACGATCACTAAATCTGTGGTTGATCGCTATTTGGTTCAATTTCGCCATAAGGTCGAAGATGCCCGTGTCCTCGATGAAGGTCCATGGCTTCATGATAACTTCCATATTATCATGCATTGTATTGCTCCTGGTGTTGTACCGAATCTTGTCCCGTTGAATCACATAAAGTTTTGGGTCCAGGTTCACGGATTGCCATTTGGTTTCATCCAGCCAAAAGTCGGTCAAGGTATAGGGAGTTTCTTAGGCACTCTTAAGGCTTATGATGTCCGCAACACCATTCACATCTCCTATATGCGAATCAAAGTGGCCATAGACGTCACTGTCCCATTGAAAAAAGAGTGGCGTGTTCGTGCCAGTAATGGTACTTTTGTTAGAGTTAACTTCAAATACGAAAAACTCGGTGTCTTTTGTCACAGGTGTGGAGTTATTGGACATACAGACAAAGTTTGCCCTGAATTGTTCGAGCTTGATTCAGATGATGGTGTCCGTAATTGGGGGCCATATCTCAAACCGGTCACTCAACAAATTGGAACTGCTGCTACCAATCGTTGGCTTCAGGACCCTATTGTTATACCCTAATTTTGGACCTAAAAATACTTGTCCAATTCTTCTTTTCGTGCATTTCAAGTCATTTGAATATGTCTCTGAGCAATTACATTTTTCTCTGCATTTTATTTCAATATTACTTGCAAAATTCACATAGAAGGGTATTTTAGTCATTTCCTATGTTAAAATTTCTTTCAATCTTTGAAAAATTGTTTGAGTCTTTTCAACTGGTTTGAATAAGTCCTTATTTCAATTCTATGTCAAAATCCATTTCAAAAAGTGCATTTGGAGTCATTTTGAGTCAGTTTAGCCCCTAGGGGCATTTTAGTCTTTTCATGTGTTAAAACCCTGTTTAAGTTCTAAAATTGTCTTTGAGTCTTTTGATTTTGCTTGTATAAGCTCTTGCTTAAATTTCCTGTTAAAAATCCATTTTAAAATTGCATCTGAGTCATTTTTAGTCGTTTTAGTCTCCAGGGGCATTTTGGTCATTTCACACGAAATTTTGGCAGAGAGAGGTTACTTTAAAGTACCTCATTTAAGTAATTGGTTCGTTTTAGTCCGTTTTGTCATTTTTAATCGTTTCACTTTACGTTATTTTCCGTTTACCAATTTTAATTCATTTTATTTTAATTTTCATTTTAAGTCCTAAATTTCCTAAAATTGTGTTTTGGTCCAGTATTGTTAATTGCAGTCCAGTCCTTGTATTTTGTCCTCTTTGCAGAAAGGGCCCATAGGTCAAAGCAGTTAGAGTTGGTCAAAGTTTGTTACATTTACAGTCCATGTGGCAGCCCATTACTGGTTCAAATATCACATGTGTCAGCTATAAATAGCCCAGTCACAGGCCAAGTCACACGGATCCATTCATTGTTGTACAACTCATCACAAACAGATTCTCTTTTCTCTCTCTCTGCATTTTCAGATCGAAACACAAAAACTCTTGATGAACTTGATGAATATTCATCAAGAACATCAAGAACAATCATAACAACTTTTTCCAGAAATCCAATCCAAACACTTCTCACTGCCATAATTCACCACCAATTCTCATAATCAACAACAACAGTAACGAATTCACTGATTCATCAACACCATTTCATCGCAAACAACCACAATATTTCCAGAAATTCATCCAGATTCACCAATTGTTCCGCAATTCATCATCTCAAAACCAAAATTCATCAAAAATCGAGTTTCTCAAACCCTAACATCCAAAATCCGAATCCAATCTCAACAATATCACCGCAAGAATTGGCATCTCAGGTTGTTCGAAAACGAACAACACGTTAA
Protein sequence

>MS.gene09517.t1

MDPTTLNLDGLTLSDDGLILNLEVKPKTAAVIDHCLIGRVLANREIQFSYFSERMSRAWKPGKRVTITKSVVDRYLVQFRHKVEDARVLDEGPWLHDNFHIIMHCIAPGVVPNLVPLNHIKFWVQVHGLPFGFIQPKVGQGIGSFLGTLKAYDVRNTIHISYMRIKVAIDVTVPLKKEWRVRASNGTFVRVNFKYEKLGVFCHRCGVIGHTDKVCPELFELDSDDGVRNWGPYLKPVTQQIGTAATNLTNSLIHQHHFIANNHNISRNSSRFTNCSAIHHLKTKIHQKSSFSNPNIQNPNPISTISPQELASQVVRKRTTR