AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76420.t1 MTR_8g463110 42.105 133 63 2 131 263 1 119 4.75e-19 87.8
MS.gene76420.t1 MTR_3g053920 32.105 190 98 5 1 188 120 280 2.49e-16 77.8
MS.gene76420.t1 MTR_0010s0370 49.123 57 27 1 4 58 121 177 5.34e-12 63.9
MS.gene76420.t1 MTR_0010s0350 49.123 57 27 1 4 58 121 177 5.34e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76420.t1 AT2G41590 28.283 99 69 1 1 97 121 219 7.81e-12 65.5
MS.gene76420.t1 AT2G17920 26.126 111 80 1 1 109 121 231 1.59e-11 64.3

Find 78 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTACAGATGTGGTCTTCT+TGG 0.172777 8.1:+30548774 MS.gene76420:CDS
CGACAAGGAAGTCCTGTTTA+AGG 0.278652 8.1:-30549067 None:intergenic
GACAATGGGGTGTCTAATTT+AGG 0.301312 8.1:+30549389 MS.gene76420:CDS
GCCTAACCTATCTAATTTGC+AGG 0.341949 8.1:+30549010 MS.gene76420:CDS
CAGGTACATGGCCTACCATT+TGG 0.344367 8.1:+30548543 MS.gene76420:CDS
AGTTCAAAGCTTATGATATT+CGG 0.373561 8.1:+30548613 MS.gene76420:CDS
AGGATGTTGGCTGCTAATAA+TGG 0.375683 8.1:+30549317 MS.gene76420:CDS
CTTCTTAATTCTTCTTCTGC+AGG 0.381849 8.1:+30549149 MS.gene76420:CDS
TCGGATGATGGCAGTGCATC+AGG 0.392285 8.1:+30549034 MS.gene76420:CDS
ATAGGTGTTACACGCCTTGC+TGG 0.404811 8.1:+30549242 MS.gene76420:CDS
TCTCCCCGCTCTCTTGTCCT+TGG 0.409261 8.1:+30549194 MS.gene76420:CDS
GGAGATCAATTCCCTTAAAC+AGG 0.414045 8.1:+30549055 MS.gene76420:CDS
TAGGAATGAATGTTAGCAAT+GGG 0.415486 8.1:+30549408 MS.gene76420:CDS
GAAGCTATTGTGTCTGCAAA+TGG 0.428063 8.1:+30549365 MS.gene76420:CDS
TCTGGTGGTGATTCTTCTGT+TGG 0.429475 8.1:+30548981 MS.gene76420:CDS
TTCTGCAGGTACCTTCCATC+AGG 0.434811 8.1:+30549163 MS.gene76420:CDS
GCAGCCCCTCTGACTATCAT+AGG 0.436271 8.1:+30549224 MS.gene76420:CDS
TAGGACACACTTTGTCTGTA+TGG 0.438093 8.1:-30548797 None:intergenic
TGTCTGCAAATGGGGACAAT+GGG 0.451752 8.1:+30549375 MS.gene76420:CDS
TACCTTGACCTACCCTCTGC+TGG 0.454509 8.1:-30548572 None:intergenic
GAAGAATTAAGAAGATGAAC+CGG 0.457930 8.1:-30549140 None:intergenic
CTCAACCGCTGAAGAAAGAA+TGG 0.464818 8.1:+30548685 MS.gene76420:CDS
TAAGCCAAGGACAAGAGAGC+GGG 0.471228 8.1:-30549198 None:intergenic
GACAAGGAAGTCCTGTTTAA+GGG 0.473079 8.1:-30549066 None:intergenic
AAGCTATTGTGTCTGCAAAT+GGG 0.475369 8.1:+30549366 MS.gene76420:CDS
TATGGAGTAGAACTCATTGC+AGG 0.478288 8.1:+30550372 MS.gene76420:CDS
TCCGACCCTGCAAATTAGAT+AGG 0.494282 8.1:-30549016 None:intergenic
CTCTGCTGGATAAAACCAAA+TGG 0.501806 8.1:-30548558 None:intergenic
TAATTTGCAGGGTCGGATGA+TGG 0.512280 8.1:+30549022 MS.gene76420:CDS
ATGATAGTCAGAGGGGCTGC+AGG 0.514811 8.1:-30549221 None:intergenic
CAGAGATGAGGAAAAGAAAG+AGG 0.519736 8.1:+30549297 MS.gene76420:CDS
AAGTTTGTGCAGCACCTACA+TGG 0.520686 8.1:-30550451 None:intergenic
AAATTGATCTCAAAGAGCTT+AGG 0.521745 8.1:-30548816 None:intergenic
TCAGGCGAACAACAAATGTC+TGG 0.522700 8.1:+30548963 MS.gene76420:intron
ACCTATCTAATTTGCAGGGT+CGG 0.525324 8.1:+30549015 MS.gene76420:CDS
GGAATGAATGTTAGCAATGG+GGG 0.525369 8.1:+30549410 MS.gene76420:CDS
TGATGTCGAAGATGATGTTA+TGG 0.528847 8.1:+30549265 MS.gene76420:CDS
AATACGATGGTGGCTCGTAA+TGG 0.530638 8.1:+30549095 MS.gene76420:CDS
GAGGAAAAGAAAGAGGATGT+TGG 0.531117 8.1:+30549304 MS.gene76420:CDS
GCGCAGAAGATATGTCACTA+TGG 0.532193 8.1:+30550354 MS.gene76420:intron
GACATGTGGGTGCAGGTACA+TGG 0.537834 8.1:+30548531 MS.gene76420:CDS
ACCTTCCATTCTTTCTTCAG+CGG 0.543960 8.1:-30548690 None:intergenic
CTTGACTTTGATGCGCATGT+AGG 0.548537 8.1:-30548652 None:intergenic
ACCTGAATCACATGGACATG+TGG 0.558407 8.1:+30548517 None:intergenic
ATGGTGGCTCGTAATGGTGC+TGG 0.563526 8.1:+30549101 MS.gene76420:CDS
GTGTCTGCAAATGGGGACAA+TGG 0.566417 8.1:+30549374 MS.gene76420:CDS
TTAGGAATGAATGTTAGCAA+TGG 0.567232 8.1:+30549407 MS.gene76420:CDS
CCCTGCAAATTAGATAGGTT+AGG 0.575997 8.1:-30549011 None:intergenic
AGAAATAAACTCCTGATGGA+AGG 0.579648 8.1:-30549174 None:intergenic
TGGTTCATCCTCTATGACAC+CGG 0.582440 8.1:+30549121 MS.gene76420:CDS
TGGAAGGTTCTTGTGAATGA+CGG 0.598922 8.1:+30548705 MS.gene76420:CDS
GCTGGATAAAACCAAATGGT+AGG 0.605643 8.1:-30548554 None:intergenic
TCACATGGACATGTGGGTGC+AGG 0.608394 8.1:+30548524 None:intergenic
ACCGCTGAAGAAAGAATGGA+AGG 0.610180 8.1:+30548689 MS.gene76420:CDS
GAGGGGCTGCAGGTAAGCCA+AGG 0.610291 8.1:-30549211 None:intergenic
AGCTATTGTGTCTGCAAATG+GGG 0.613366 8.1:+30549367 MS.gene76420:CDS
TTGGCTGCTAATAATGGAAA+GGG 0.613420 8.1:+30549323 MS.gene76420:CDS
GGGGAGAAATAAACTCCTGA+TGG 0.616093 8.1:-30549178 None:intergenic
AGGAATGAATGTTAGCAATG+GGG 0.617577 8.1:+30549409 MS.gene76420:CDS
AAGATGAACCGGTGTCATAG+AGG 0.620529 8.1:-30549129 None:intergenic
GTAAGCCAAGGACAAGAGAG+CGG 0.626942 8.1:-30549199 None:intergenic
TAACACCTATGATAGTCAGA+GGG 0.627978 8.1:-30549229 None:intergenic
GTTGGCTGCTAATAATGGAA+AGG 0.628067 8.1:+30549322 MS.gene76420:CDS
CCATCGTATTCTGAGCGACA+AGG 0.632753 8.1:-30549082 None:intergenic
CTGTTGCGTGATAACCATGT+AGG 0.633555 8.1:+30550437 MS.gene76420:CDS
ATCCAGCAGAGGGTAGGTCA+AGG 0.643113 8.1:+30548570 MS.gene76420:CDS
CCTAACCTATCTAATTTGCA+GGG 0.644193 8.1:+30549011 MS.gene76420:CDS
GTAACACCTATGATAGTCAG+AGG 0.644353 8.1:-30549230 None:intergenic
AAGCCAAGGACAAGAGAGCG+GGG 0.654110 8.1:-30549197 None:intergenic
TGGATACCGAATCAGAGATG+AGG 0.654612 8.1:+30549285 MS.gene76420:CDS
CATCTTCGACATCACCAGCA+AGG 0.655768 8.1:-30549256 None:intergenic
GAATGAATGTTAGCAATGGG+GGG 0.656964 8.1:+30549411 MS.gene76420:CDS
TGTCGCTCAGAATACGATGG+TGG 0.659022 8.1:+30549085 MS.gene76420:CDS
GTCTGCAAATGGGGACAATG+GGG 0.665514 8.1:+30549376 MS.gene76420:CDS
GGCGAACAACAAATGTCTGG+TGG 0.668228 8.1:+30548966 MS.gene76420:intron
CCTGAATCACATGGACATGT+GGG 0.695568 8.1:+30548518 None:intergenic
CCTTGTCGCTCAGAATACGA+TGG 0.697317 8.1:+30549082 MS.gene76420:CDS
AACACCTATGATAGTCAGAG+GGG 0.752311 8.1:-30549228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAAAACTTCAATTTTATTT+CGG - chr8.1:30549642-30549661 None:intergenic 15.0%
!!! TTTTTCAAATATGAAAAACT+CGG + chr8.1:30548744-30548763 MS.gene76420:CDS 15.0%
!! AATTTATCTAATCAATATGC+AGG + chr8.1:30549886-30549905 MS.gene76420:intron 20.0%
!! ATTGATTAGATAAATTACGA+AGG - chr8.1:30549882-30549901 None:intergenic 20.0%
!! TTCAATAGTTATATGATTGT+TGG - chr8.1:30549773-30549792 None:intergenic 20.0%
!!! TTTTCATATTTGAAAAACAC+TGG - chr8.1:30548741-30548760 None:intergenic 20.0%
! AATTCAAGTATGAAAATGCA+TGG + chr8.1:30550246-30550265 MS.gene76420:intron 25.0%
! AGTTCAAAGCTTATGATATT+CGG + chr8.1:30548613-30548632 MS.gene76420:CDS 25.0%
! GATATCTGATATGAATGTAA+TGG + chr8.1:30549433-30549452 MS.gene76420:intron 25.0%
! TCACTTAAGAATAATAGATC+TGG - chr8.1:30549607-30549626 None:intergenic 25.0%
!! GAAGTTTTTCGTTATATTCT+TGG + chr8.1:30549652-30549671 MS.gene76420:intron 25.0%
!! TTCATACTTGAATTTTGTCT+AGG - chr8.1:30550240-30550259 None:intergenic 25.0%
!!! AAACTTCAATTTTATTTCGG+TGG - chr8.1:30549639-30549658 None:intergenic 25.0%
!!! GAAATTATTGACTCTATTCT+TGG + chr8.1:30549790-30549809 MS.gene76420:intron 25.0%
!!! TTTGAGATCAATTTTGATGA+TGG + chr8.1:30548825-30548844 MS.gene76420:CDS 25.0%
AAACTCTTAAACCAAGTGAA+CGG - chr8.1:30550057-30550076 None:intergenic 30.0%
AAATTGATCTCAAAGAGCTT+AGG - chr8.1:30548819-30548838 None:intergenic 30.0%
CAGATTAGGTACTTTAATTC+TGG - chr8.1:30549574-30549593 None:intergenic 30.0%
GAAGAATTAAGAAGATGAAC+CGG - chr8.1:30549143-30549162 None:intergenic 30.0%
GTTTGATGAGTATGTTGTAT+CGG + chr8.1:30549466-30549485 MS.gene76420:intron 30.0%
TAGGAATGAATGTTAGCAAT+GGG + chr8.1:30549408-30549427 MS.gene76420:CDS 30.0%
TTAGGAATGAATGTTAGCAA+TGG + chr8.1:30549407-30549426 MS.gene76420:CDS 30.0%
TTCACTTGGTTTAAGAGTTT+GGG + chr8.1:30550057-30550076 MS.gene76420:intron 30.0%
! AGATTTTCTAACATGGCATT+TGG - chr8.1:30550150-30550169 None:intergenic 30.0%
! ATACTTGAATTTTGTCTAGG+AGG - chr8.1:30550237-30550256 None:intergenic 30.0%
! CAGAATTAAAGTACCTAATC+TGG + chr8.1:30549572-30549591 MS.gene76420:intron 30.0%
!! ATGATATAGTCACTGATTCT+TGG + chr8.1:30550288-30550307 MS.gene76420:intron 30.0%
!!! AATTTTATTTCGGTGGACTA+AGG - chr8.1:30549632-30549651 None:intergenic 30.0%
AAGCTATTGTGTCTGCAAAT+GGG + chr8.1:30549366-30549385 MS.gene76420:CDS 35.0%
AAGTCATCAATAATGCACCA+AGG - chr8.1:30549913-30549932 None:intergenic 35.0%
ACAACATACTCATCAAACAG+TGG - chr8.1:30549464-30549483 None:intergenic 35.0%
AGAAATAAACTCCTGATGGA+AGG - chr8.1:30549177-30549196 None:intergenic 35.0%
AGGAATGAATGTTAGCAATG+GGG + chr8.1:30549409-30549428 MS.gene76420:CDS 35.0%
CTTCTTAATTCTTCTTCTGC+AGG + chr8.1:30549149-30549168 MS.gene76420:CDS 35.0%
CTTTGAACTCACCCAAAAAT+TGG - chr8.1:30548602-30548621 None:intergenic 35.0%
GTTCACTTGGTTTAAGAGTT+TGG + chr8.1:30550056-30550075 MS.gene76420:intron 35.0%
TAACACCTATGATAGTCAGA+GGG - chr8.1:30549232-30549251 None:intergenic 35.0%
TGATGACTTCAACGACATAA+TGG + chr8.1:30549924-30549943 MS.gene76420:intron 35.0%
TGATGTCGAAGATGATGTTA+TGG + chr8.1:30549265-30549284 MS.gene76420:CDS 35.0%
! AAGATGAAACCAAGCATTGT+TGG - chr8.1:30550124-30550143 None:intergenic 35.0%
! ACTATCCAATTTTGTTGCAC+CGG + chr8.1:30550190-30550209 MS.gene76420:intron 35.0%
! CCTAACCTATCTAATTTGCA+GGG + chr8.1:30549011-30549030 MS.gene76420:CDS 35.0%
! TCTTTTCCTCATCTCTGATT+CGG - chr8.1:30549294-30549313 None:intergenic 35.0%
! TTGGCTGCTAATAATGGAAA+GGG + chr8.1:30549323-30549342 MS.gene76420:CDS 35.0%
!! CAAGGTATCAGCCAATTTTT+GGG + chr8.1:30548588-30548607 MS.gene76420:CDS 35.0%
!! TCAAGGTATCAGCCAATTTT+TGG + chr8.1:30548587-30548606 MS.gene76420:CDS 35.0%
!!! AATTTTGATGATGGTGTGTG+AGG + chr8.1:30548834-30548853 MS.gene76420:intron 35.0%
!!! ATTTGGTTTTATCCAGCAGA+GGG + chr8.1:30548560-30548579 MS.gene76420:CDS 35.0%
!!! ATTTTGATGATGGTGTGTGA+GGG + chr8.1:30548835-30548854 MS.gene76420:intron 35.0%
AACACCTATGATAGTCAGAG+GGG - chr8.1:30549231-30549250 None:intergenic 40.0%
AAGCCGTTAATTAACCATCG+CGG - chr8.1:30549982-30550001 None:intergenic 40.0%
ACCTTCCATTCTTTCTTCAG+CGG - chr8.1:30548693-30548712 None:intergenic 40.0%
AGCTACTAACAAATGGCAGT+AGG + chr8.1:30548902-30548921 MS.gene76420:intron 40.0%
AGCTATTGTGTCTGCAAATG+GGG + chr8.1:30549367-30549386 MS.gene76420:CDS 40.0%
ATTCCATGCTACAACACTCA+TGG - chr8.1:30549521-30549540 None:intergenic 40.0%
CAACCGCGATGGTTAATTAA+CGG + chr8.1:30549976-30549995 MS.gene76420:intron 40.0%
CAGAGATGAGGAAAAGAAAG+AGG + chr8.1:30549297-30549316 MS.gene76420:CDS 40.0%
CCCTGCAAATTAGATAGGTT+AGG - chr8.1:30549014-30549033 None:intergenic 40.0%
CTAATCAATATGCAGGTCCT+TGG + chr8.1:30549893-30549912 MS.gene76420:intron 40.0%
CTCTGCTGGATAAAACCAAA+TGG - chr8.1:30548561-30548580 None:intergenic 40.0%
GAAGCTATTGTGTCTGCAAA+TGG + chr8.1:30549365-30549384 MS.gene76420:CDS 40.0%
GAATGAATGTTAGCAATGGG+GGG + chr8.1:30549411-30549430 MS.gene76420:CDS 40.0%
GACAAGGAAGTCCTGTTTAA+GGG - chr8.1:30549069-30549088 None:intergenic 40.0%
GAGAATTCACATCAGGGATA+GGG - chr8.1:30548929-30548948 None:intergenic 40.0%
GAGGAAAAGAAAGAGGATGT+TGG + chr8.1:30549304-30549323 MS.gene76420:CDS 40.0%
GCTGGATAAAACCAAATGGT+AGG - chr8.1:30548557-30548576 None:intergenic 40.0%
GGAATGAATGTTAGCAATGG+GGG + chr8.1:30549410-30549429 MS.gene76420:CDS 40.0%
GGAGATCAATTCCCTTAAAC+AGG + chr8.1:30549055-30549074 MS.gene76420:CDS 40.0%
GGTGCAACAAAATTGGATAG+TGG - chr8.1:30550191-30550210 None:intergenic 40.0%
GTAACACCTATGATAGTCAG+AGG - chr8.1:30549233-30549252 None:intergenic 40.0%
GTACTGCAGCTACTAACAAA+TGG + chr8.1:30548895-30548914 MS.gene76420:intron 40.0%
GTGACTATATCATGAAAGCC+TGG - chr8.1:30550281-30550300 None:intergenic 40.0%
TAGGACACACTTTGTCTGTA+TGG - chr8.1:30548800-30548819 None:intergenic 40.0%
TATGGAGTAGAACTCATTGC+AGG + chr8.1:30550372-30550391 MS.gene76420:CDS 40.0%
TGGAAGGTTCTTGTGAATGA+CGG + chr8.1:30548705-30548724 MS.gene76420:CDS 40.0%
TTGACTCTATTCTTGGCACT+TGG + chr8.1:30549797-30549816 MS.gene76420:intron 40.0%
! AAACCAAGCATTGTTGGCAA+GGG - chr8.1:30550118-30550137 None:intergenic 40.0%
! ACCTATCTAATTTGCAGGGT+CGG + chr8.1:30549015-30549034 MS.gene76420:CDS 40.0%
! AGGATGTTGGCTGCTAATAA+TGG + chr8.1:30549317-30549336 MS.gene76420:CDS 40.0%
! ATTGGATAGTGGTATGAAGC+CGG - chr8.1:30550180-30550199 None:intergenic 40.0%
! CTTGAATTTTGTCTAGGAGG+AGG - chr8.1:30550234-30550253 None:intergenic 40.0%
! GACAATGGGGTGTCTAATTT+AGG + chr8.1:30549389-30549408 MS.gene76420:CDS 40.0%
! GCCTAACCTATCTAATTTGC+AGG + chr8.1:30549010-30549029 MS.gene76420:CDS 40.0%
! GGCAGCAAGATTTTCTAACA+TGG - chr8.1:30550157-30550176 None:intergenic 40.0%
! GTTGGCTGCTAATAATGGAA+AGG + chr8.1:30549322-30549341 MS.gene76420:CDS 40.0%
!! TGCTTTTATGTTGACTGCAC+CGG + chr8.1:30549685-30549704 MS.gene76420:intron 40.0%
!!! CATTTGGTTTTATCCAGCAG+AGG + chr8.1:30548559-30548578 MS.gene76420:CDS 40.0%
!!! GGTGTTTTTTGCTACAGATG+TGG + chr8.1:30548765-30548784 MS.gene76420:CDS 40.0%
ACCGCTGAAGAAAGAATGGA+AGG + chr8.1:30548689-30548708 MS.gene76420:CDS 45.0%
ACTCTATTCTTGGCACTTGG+AGG + chr8.1:30549800-30549819 MS.gene76420:intron 45.0%
ATCCCTGATGTGAATTCTCG+TGG + chr8.1:30548930-30548949 MS.gene76420:intron 45.0%
ATGGATGACAGTGAGAAGAG+AGG + chr8.1:30549943-30549962 MS.gene76420:intron 45.0%
ATTACGAAGGAAGTTCCACG+CGG - chr8.1:30549869-30549888 None:intergenic 45.0%
CGACAAGGAAGTCCTGTTTA+AGG - chr8.1:30549070-30549089 None:intergenic 45.0%
CGAGAATTCACATCAGGGAT+AGG - chr8.1:30548930-30548949 None:intergenic 45.0%
CTCAACCGCTGAAGAAAGAA+TGG + chr8.1:30548685-30548704 MS.gene76420:CDS 45.0%
CTTGACTTTGATGCGCATGT+AGG - chr8.1:30548655-30548674 None:intergenic 45.0%
GCGCAGAAGATATGTCACTA+TGG + chr8.1:30550354-30550373 MS.gene76420:intron 45.0%
GGGGAGAAATAAACTCCTGA+TGG - chr8.1:30549181-30549200 None:intergenic 45.0%
GTGTTGTAGCATGGAATTGC+CGG + chr8.1:30549524-30549543 MS.gene76420:intron 45.0%
GTTAGAAAATCTTGCTGCCC+CGG + chr8.1:30550158-30550177 MS.gene76420:intron 45.0%
TAGGACCGGTGCAACAAAAT+TGG - chr8.1:30550198-30550217 None:intergenic 45.0%
TCAGGCGAACAACAAATGTC+TGG + chr8.1:30548963-30548982 MS.gene76420:intron 45.0%
TCCGACCCTGCAAATTAGAT+AGG - chr8.1:30549019-30549038 None:intergenic 45.0%
TCTCGTGGCAGTACTAATTC+AGG + chr8.1:30548945-30548964 MS.gene76420:intron 45.0%
TCTGCGCATGCAGTAAGTTT+AGG - chr8.1:30550341-30550360 None:intergenic 45.0%
TGCCACGAGAATTCACATCA+GGG - chr8.1:30548935-30548954 None:intergenic 45.0%
TGCTACAGATGTGGTCTTCT+TGG + chr8.1:30548774-30548793 MS.gene76420:CDS 45.0%
TGGATACCGAATCAGAGATG+AGG + chr8.1:30549285-30549304 MS.gene76420:CDS 45.0%
TGGTTCATCCTCTATGACAC+CGG + chr8.1:30549121-30549140 MS.gene76420:CDS 45.0%
TGTCTGCAAATGGGGACAAT+GGG + chr8.1:30549375-30549394 MS.gene76420:CDS 45.0%
TGTTGTAGCATGGAATTGCC+GGG + chr8.1:30549525-30549544 MS.gene76420:intron 45.0%
! AAGCATTGTTGGCAAGGGAT+CGG - chr8.1:30550113-30550132 None:intergenic 45.0%
! AATACGATGGTGGCTCGTAA+TGG + chr8.1:30549095-30549114 MS.gene76420:CDS 45.0%
! GAAACCAAGCATTGTTGGCA+AGG - chr8.1:30550119-30550138 None:intergenic 45.0%
! GATGAGTATGTTGTATCGGC+AGG + chr8.1:30549470-30549489 MS.gene76420:intron 45.0%
! TAATTTGCAGGGTCGGATGA+TGG + chr8.1:30549022-30549041 MS.gene76420:CDS 45.0%
! TCTGGTGGTGATTCTTCTGT+TGG + chr8.1:30548981-30549000 MS.gene76420:CDS 45.0%
! TTGGATAGTGGTATGAAGCC+GGG - chr8.1:30550179-30550198 None:intergenic 45.0%
!! AAGATGAACCGGTGTCATAG+AGG - chr8.1:30549132-30549151 None:intergenic 45.0%
!! CGTCAAGCTGTTTTAGACTC+GGG + chr8.1:30550003-30550022 MS.gene76420:intron 45.0%
!! GCGTCAAGCTGTTTTAGACT+CGG + chr8.1:30550002-30550021 MS.gene76420:intron 45.0%
!! TAGAAGGGTATCCGTTCACT+TGG + chr8.1:30550043-30550062 MS.gene76420:intron 45.0%
AAACCAGTAGCTCGTCGTGT+TGG + chr8.1:30548873-30548892 MS.gene76420:intron 50.0%
AATGCATGGCAGCTTGAACC+AGG + chr8.1:30550260-30550279 MS.gene76420:intron 50.0%
AGTCTCTCTTCAACAGCTCG+TGG - chr8.1:30550087-30550106 None:intergenic 50.0%
CAGGTACATGGCCTACCATT+TGG + chr8.1:30548543-30548562 MS.gene76420:CDS 50.0%
CATCTTCGACATCACCAGCA+AGG - chr8.1:30549259-30549278 None:intergenic 50.0%
CATGTCTACGTTCCCCATTG+GGG - chr8.1:30549846-30549865 None:intergenic 50.0%
CCATCGTATTCTGAGCGACA+AGG - chr8.1:30549085-30549104 None:intergenic 50.0%
CCTTGTCGCTCAGAATACGA+TGG + chr8.1:30549082-30549101 MS.gene76420:CDS 50.0%
CTGCCACGAGAATTCACATC+AGG - chr8.1:30548936-30548955 None:intergenic 50.0%
GATCCCTTGCCAACAATGCT+TGG + chr8.1:30550112-30550131 MS.gene76420:intron 50.0%
GCATGTCTACGTTCCCCATT+GGG - chr8.1:30549847-30549866 None:intergenic 50.0%
GGCGAACAACAAATGTCTGG+TGG + chr8.1:30548966-30548985 MS.gene76420:intron 50.0%
GTAAGCCAAGGACAAGAGAG+CGG - chr8.1:30549202-30549221 None:intergenic 50.0%
GTCTGCAAATGGGGACAATG+GGG + chr8.1:30549376-30549395 MS.gene76420:CDS 50.0%
GTGAACGGATACCCTTCTAG+AGG - chr8.1:30550042-30550061 None:intergenic 50.0%
GTGTCTGCAAATGGGGACAA+TGG + chr8.1:30549374-30549393 MS.gene76420:CDS 50.0%
GTTACTACGGGTACCCCAAT+GGG + chr8.1:30549830-30549849 MS.gene76420:intron 50.0%
GTTGTAGCATGGAATTGCCG+GGG + chr8.1:30549526-30549545 MS.gene76420:intron 50.0%
TAAGCCAAGGACAAGAGAGC+GGG - chr8.1:30549201-30549220 None:intergenic 50.0%
TGTCGCTCAGAATACGATGG+TGG + chr8.1:30549085-30549104 MS.gene76420:CDS 50.0%
TTACTACGGGTACCCCAATG+GGG + chr8.1:30549831-30549850 MS.gene76420:intron 50.0%
TTCTGCAGGTACCTTCCATC+AGG + chr8.1:30549163-30549182 MS.gene76420:CDS 50.0%
TTGTCAGACGTGCCTCTAGA+AGG + chr8.1:30550027-30550046 MS.gene76420:intron 50.0%
! ATAGGTGTTACACGCCTTGC+TGG + chr8.1:30549242-30549261 MS.gene76420:CDS 50.0%
! ATTGGGGTACCCGTAGTAAC+CGG - chr8.1:30549830-30549849 None:intergenic 50.0%
! CTGGATTGAAGCGCCAGATT+AGG - chr8.1:30549588-30549607 None:intergenic 50.0%
! GGTCCATGAGTGTTGTAGCA+TGG + chr8.1:30549515-30549534 MS.gene76420:intron 50.0%
! TATGTTGTATCGGCAGGTCC+TGG + chr8.1:30549476-30549495 MS.gene76420:intron 50.0%
! TGGAGGCTTACCGGTTACTA+CGG + chr8.1:30549817-30549836 MS.gene76420:intron 50.0%
! TGGATAGTGGTATGAAGCCG+GGG - chr8.1:30550178-30550197 None:intergenic 50.0%
! TGTCAGACGTGCCTCTAGAA+GGG + chr8.1:30550028-30550047 MS.gene76420:intron 50.0%
!! GCGGGGGTCTTGCTATATTT+TGG + chr8.1:30549713-30549732 MS.gene76420:intron 50.0%
!! GGTTTTATCCAGCAGAGGGT+AGG + chr8.1:30548564-30548583 MS.gene76420:CDS 50.0%
!! TGATGATGGTGTGTGAGGGT+GGG + chr8.1:30548839-30548858 MS.gene76420:intron 50.0%
!! TTGATGATGGTGTGTGAGGG+TGG + chr8.1:30548838-30548857 MS.gene76420:intron 50.0%
AAGCCAAGGACAAGAGAGCG+GGG - chr8.1:30549200-30549219 None:intergenic 55.0%
ATCCAGCAGAGGGTAGGTCA+AGG + chr8.1:30548570-30548589 MS.gene76420:CDS 55.0%
ATGATAGTCAGAGGGGCTGC+AGG - chr8.1:30549224-30549243 None:intergenic 55.0%
ATGGTGGCTCGTAATGGTGC+TGG + chr8.1:30549101-30549120 MS.gene76420:CDS 55.0%
CTTGGCACTTGGAGGCTTAC+CGG + chr8.1:30549808-30549827 MS.gene76420:intron 55.0%
GACATGTGGGTGCAGGTACA+TGG + chr8.1:30548531-30548550 MS.gene76420:CDS 55.0%
GAGCACGAGGCATATAGGAC+CGG - chr8.1:30550212-30550231 None:intergenic 55.0%
GCAGCCCCTCTGACTATCAT+AGG + chr8.1:30549224-30549243 MS.gene76420:CDS 55.0%
GCATGGAATTGCCGGGGTTT+AGG + chr8.1:30549532-30549551 MS.gene76420:intron 55.0%
GGCATGTCTACGTTCCCCAT+TGG - chr8.1:30549848-30549867 None:intergenic 55.0%
GGTTACTACGGGTACCCCAA+TGG + chr8.1:30549829-30549848 MS.gene76420:intron 55.0%
GTACCAACACGACGAGCTAC+TGG - chr8.1:30548879-30548898 None:intergenic 55.0%
TACCTTGACCTACCCTCTGC+TGG - chr8.1:30548575-30548594 None:intergenic 55.0%
! AGGAGGAGCACGAGGCATAT+AGG - chr8.1:30550217-30550236 None:intergenic 55.0%
! GATGATGGTGTGTGAGGGTG+GGG + chr8.1:30548840-30548859 MS.gene76420:intron 55.0%
! GGAGGCTTACCGGTTACTAC+GGG + chr8.1:30549818-30549837 MS.gene76420:intron 55.0%
! GTTGACTGCACCGGTAGAAG+CGG + chr8.1:30549694-30549713 MS.gene76420:intron 55.0%
! TCGGATGATGGCAGTGCATC+AGG + chr8.1:30549034-30549053 MS.gene76420:CDS 55.0%
! TTGACTGCACCGGTAGAAGC+GGG + chr8.1:30549695-30549714 MS.gene76420:intron 55.0%
!! CACGACGAGCTACTGGTTTG+AGG - chr8.1:30548872-30548891 None:intergenic 55.0%
!! CGAGCTACTGGTTTGAGGTC+AGG - chr8.1:30548867-30548886 None:intergenic 55.0%
AGCAAGACCCCCGCTTCTAC+CGG - chr8.1:30549707-30549726 None:intergenic 60.0%
ATCGGCAGGTCCTGGAACTC+AGG + chr8.1:30549484-30549503 MS.gene76420:intron 60.0%
CAACACTCATGGACCCCAGC+AGG - chr8.1:30549510-30549529 None:intergenic 60.0%
CACTCGAGCGACCTAAACCC+CGG - chr8.1:30549546-30549565 None:intergenic 60.0%
GACGTGCTCTACAACCGCGA+TGG + chr8.1:30549965-30549984 MS.gene76420:intron 60.0%
TCCTGGAACTCAGGCCTGCT+GGG + chr8.1:30549493-30549512 MS.gene76420:intron 60.0%
TCTCCCCGCTCTCTTGTCCT+TGG + chr8.1:30549194-30549213 MS.gene76420:CDS 60.0%
! TGACTGCACCGGTAGAAGCG+GGG + chr8.1:30549696-30549715 MS.gene76420:intron 60.0%
! TGTCTAGGAGGAGGAGCACG+AGG - chr8.1:30550225-30550244 None:intergenic 60.0%
CCTGGAACTCAGGCCTGCTG+GGG + chr8.1:30549494-30549513 MS.gene76420:intron 65.0%
GAGGGGCTGCAGGTAAGCCA+AGG - chr8.1:30549214-30549233 None:intergenic 65.0%
GGGAACGTAGACATGCCGCG+TGG + chr8.1:30549851-30549870 MS.gene76420:intron 65.0%
GTCCTGGAACTCAGGCCTGC+TGG + chr8.1:30549492-30549511 MS.gene76420:intron 65.0%
! GACTGCACCGGTAGAAGCGG+GGG + chr8.1:30549697-30549716 MS.gene76420:intron 65.0%
CCCCAGCAGGCCTGAGTTCC+AGG - chr8.1:30549497-30549516 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 30548528 30550458 30548528 ID=MS.gene76420
chr8.1 mRNA 30548528 30550458 30548528 ID=MS.gene76420.t1;Parent=MS.gene76420
chr8.1 exon 30548528 30548846 30548528 ID=MS.gene76420.t1.exon1;Parent=MS.gene76420.t1
chr8.1 CDS 30548528 30548846 30548528 ID=cds.MS.gene76420.t1;Parent=MS.gene76420.t1
chr8.1 exon 30548967 30549448 30548967 ID=MS.gene76420.t1.exon2;Parent=MS.gene76420.t1
chr8.1 CDS 30548967 30549448 30548967 ID=cds.MS.gene76420.t1;Parent=MS.gene76420.t1
chr8.1 exon 30550360 30550458 30550360 ID=MS.gene76420.t1.exon3;Parent=MS.gene76420.t1
chr8.1 CDS 30550360 30550458 30550360 ID=cds.MS.gene76420.t1;Parent=MS.gene76420.t1
Gene Sequence

>MS.gene76420

ATGGACATGTGGGTGCAGGTACATGGCCTACCATTTGGTTTTATCCAGCAGAGGGTAGGTCAAGGTATCAGCCAATTTTTGGGTGAGTTCAAAGCTTATGATATTCGGAATACTGTGCACAGCTCCTACATGCGCATCAAAGTCAAGCTGAATGTTACTCAACCGCTGAAGAAAGAATGGAAGGTTCTTGTGAATGACGGAAACTATGTACCAGTGTTTTTCAAATATGAAAAACTCGGTGTTTTTTGCTACAGATGTGGTCTTCTTGGCCATACAGACAAAGTGTGTCCTAAGCTCTTTGAGATCAATTTTGATGATGGTGTGTGAGGGTGGGGACCTGACCTCAAACCAGTAGCTCGTCGTGTTGGTACTGCAGCTACTAACAAATGGCAGTAGGACCCTATCCCTGATGTGAATTCTCGTGGCAGTACTAATTCAGGCGAACAACAAATGTCTGGTGGTGATTCTTCTGTTGGTGTTGCGCCTAACCTATCTAATTTGCAGGGTCGGATGATGGCAGTGCATCAGGAGATCAATTCCCTTAAACAGGACTTCCTTGTCGCTCAGAATACGATGGTGGCTCGTAATGGTGCTGGTTCATCCTCTATGACACCGGTTCATCTTCTTAATTCTTCTTCTGCAGGTACCTTCCATCAGGAGTTTATTTCTCCCCGCTCTCTTGTCCTTGGCTTACCTGCAGCCCCTCTGACTATCATAGGTGTTACACGCCTTGCTGGTGATGTCGAAGATGATGTTATGGATACCGAATCAGAGATGAGGAAAAGAAAGAGGATGTTGGCTGCTAATAATGGAAAGGGTGTAGCTGTTGAATGTGCTGAAGCTATTGTGTCTGCAAATGGGGACAATGGGGTGTCTAATTTAGGAATGAATGTTAGCAATGGGGGGATATCTGATATGAATGTAATGGATAATCCACTGTTTGATGAGTATGTTGTATCGGCAGGTCCTGGAACTCAGGCCTGCTGGGGTCCATGAGTGTTGTAGCATGGAATTGCCGGGGTTTAGGTCGCTCGAGTGTGATTCCAGAATTAAAGTACCTAATCTGGCGCTTCAATCCAGATCTATTATTCTTAAGTGAGACCTTAGTCCACCGAAATAAAATTGAAGTTTTTCGTTATATTCTTGGTTATGATTCTTGCTTTTATGTTGACTGCACCGGTAGAAGCGGGGGTCTTGCTATATTTTGGTGTTCTTCTTTAAATTGCAATCTTATAAATTACTCCAACAATCATATAACTATTGAAATTATTGACTCTATTCTTGGCACTTGGAGGCTTACCGGTTACTACGGGTACCCCAATGGGGAACGTAGACATGCCGCGTGGAACTTCCTTCGTAATTTATCTAATCAATATGCAGGTCCTTGGTGCATTATTGATGACTTCAACGACATAATGGATGACAGTGAGAAGAGAGGACGTGCTCTACAACCGCGATGGTTAATTAACGGCTTGCGTCAAGCTGTTTTAGACTCGGGTTTGTCAGACGTGCCTCTAGAAGGGTATCCGTTCACTTGGTTTAAGAGTTTGGGAACTCCACGAGCTGTTGAAGAGAGACTAGACCGATCCCTTGCCAACAATGCTTGGTTTCATCTTTTTCCAAATGCCATGTTAGAAAATCTTGCTGCCCCGGCTTCATACCACTATCCAATTTTGTTGCACCGGTCCTATATGCCTCGTGCTCCTCCTCCTAGACAAAATTCAAGTATGAAAATGCATGGCAGCTTGAACCAGGCTTTCATGATATAGTCACTGATTCTTGGAACATGAATTTATCTCATCTCATCATTCCTAAACTTACTGCATGCGCAGAAGATATGTCACTATGGAGTAGAACTCATTGCAGGAAACTTAAAACTGACATTGAAGAGTGCTCACGACAACTGCAGCTGTTGCGTGATAACCATGTAG
Protein sequence

>MS.gene76420.t1

MDMWVQVHGLPFGFIQQRVGQGISQFLGEFKAYDIRNTVHSSYMRIKVKLNVTQPLKKEWKVLVNDGNYVPVFFKYEKLGVFCYRCGLLGHTDKVCPKLFEINFDDGEQQMSGGDSSVGVAPNLSNLQGRMMAVHQEINSLKQDFLVAQNTMVARNGAGSSSMTPVHLLNSSSAGTFHQEFISPRSLVLGLPAAPLTIIGVTRLAGDVEDDVMDTESEMRKRKRMLAANNGKGVAVECAEAIVSANGDNGVSNLGMNVSNGGISDMNKICHYGVELIAGNLKLTLKSAHDNCSCCVITM