AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049512


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049512.t1 MTR_0010s0370 52.247 178 84 1 1 178 4 180 1.36e-61 192
MS.gene049512.t1 MTR_0010s0350 52.247 178 84 1 1 178 4 180 1.36e-61 192
MS.gene049512.t1 MTR_1g036720 47.222 108 56 1 1 108 4 110 9.65e-28 102
MS.gene049512.t1 MTR_6g033215 30.857 175 120 1 23 196 120 294 1.81e-27 107
MS.gene049512.t1 MTR_3g053920 38.849 139 68 2 31 169 35 156 4.49e-26 103
MS.gene049512.t1 MTR_7g089990 44.915 118 62 2 101 215 85 202 1.61e-24 97.8
MS.gene049512.t1 MTR_2g061500 41.304 92 54 0 32 123 36 127 2.65e-19 81.6
MS.gene049512.t1 MTR_4g103530 39.326 89 54 0 55 143 5 93 5.50e-17 77.0
MS.gene049512.t1 MTR_1g036710 60.938 64 24 1 130 192 1 64 1.15e-16 77.8
MS.gene049512.t1 MTR_7g106180 24.658 146 103 2 32 174 26 167 8.93e-13 66.2
MS.gene049512.t1 MTR_7g011710 32.955 88 59 0 51 138 1 88 9.22e-13 64.7
MS.gene049512.t1 MTR_3g024240 32.520 123 80 1 78 197 53 175 5.75e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTTCATCGGTGAGTTTA+AGG 0.198568 3.2:+57120905 MS.gene049512:CDS
AGGAGAAGATATCAGAATTT+TGG 0.244702 3.2:+57120631 MS.gene049512:CDS
TCTCCTTCATCATATGGTTT+CGG 0.331793 3.2:-57120614 None:intergenic
ATATCTTCTCCTTCATCATA+TGG 0.332832 3.2:-57120620 None:intergenic
AATGCCTTTGTTTCTTTCTA+CGG 0.339781 3.2:-57121214 None:intergenic
CCCTCAACCATGTACCCTAT+TGG 0.355933 3.2:+57120823 MS.gene049512:CDS
CAGGGTCCTTGGTCTTTAGA+TGG 0.366959 3.2:+57120747 MS.gene049512:CDS
CATCATATGGATTCGAGAAT+AGG 0.367591 3.2:-57121130 None:intergenic
TGTCTCTCAGATGAAAGAAT+TGG 0.385537 3.2:-57120707 None:intergenic
ATGATGATGGAGTCCGGTTA+TGG 0.387406 3.2:+57121147 MS.gene049512:CDS
CCTCAACCATGTACCCTATT+GGG 0.400791 3.2:+57120824 MS.gene049512:CDS
TTGATAGCCACTTTACAAAC+AGG 0.422812 3.2:-57120657 None:intergenic
CAGACCTTCGTGCAGATAAG+AGG 0.423821 3.2:+57121174 MS.gene049512:CDS
ATGCCTTTGTTTCTTTCTAC+GGG 0.434930 3.2:-57121213 None:intergenic
ATCTTCTTCATCCGCATCAA+TGG 0.437897 3.2:-57121002 None:intergenic
TCATGAACTTGCACCCAATA+GGG 0.455286 3.2:-57120837 None:intergenic
CTTGGTTGGGAGATTCTTGA+CGG 0.462561 3.2:+57120581 MS.gene049512:CDS
AACCGGACTCCATCATCATA+TGG 0.465139 3.2:-57121143 None:intergenic
TGTTATTACTGTGGCATGCT+CGG 0.466214 3.2:+57121083 MS.gene049512:CDS
CCCAATAGGGTACATGGTTG+AGG 0.473432 3.2:-57120824 None:intergenic
AACCATGCCCTATGCTTGGT+TGG 0.480596 3.2:+57120567 MS.gene049512:CDS
GAAGGTCTGAGCTCCATAAC+CGG 0.487520 3.2:-57121160 None:intergenic
GTCATGAACTTGCACCCAAT+AGG 0.488903 3.2:-57120838 None:intergenic
ACCATGCCCTATGCTTGGTT+GGG 0.491826 3.2:+57120568 MS.gene049512:CDS
TTGGCAACCTGTTTGTAAAG+TGG 0.492639 3.2:+57120650 MS.gene049512:CDS
TTTGTTTCTTTCTACGGGAG+AGG 0.496405 3.2:-57121208 None:intergenic
TGGTTGAGGGGAACCGTTGT+GGG 0.497894 3.2:-57120810 None:intergenic
ATTACGATTGTTGACTCTCC+AGG 0.502876 3.2:+57120531 MS.gene049512:CDS
GGTTGTATGTCGCAGACTGC+AGG 0.512770 3.2:+57120870 MS.gene049512:CDS
CTTGCACCCAATAGGGTACA+TGG 0.519598 3.2:-57120830 None:intergenic
TTCTCGAATCCATATGATGA+TGG 0.523932 3.2:+57121134 MS.gene049512:CDS
ATCCATATGATGATGGAGTC+CGG 0.525293 3.2:+57121141 MS.gene049512:CDS
CCAATAGGGTACATGGTTGA+GGG 0.528920 3.2:-57120823 None:intergenic
AGAAAACCATGCCCTATGCT+TGG 0.536606 3.2:+57120563 MS.gene049512:CDS
GGAACTTTCTGTTATTACTG+TGG 0.539075 3.2:+57121074 MS.gene049512:CDS
AGAATCTCCCAACCAAGCAT+AGG 0.542532 3.2:-57120575 None:intergenic
GAATCTCCCAACCAAGCATA+GGG 0.546866 3.2:-57120574 None:intergenic
CAGACTGCAGGAAAACAACT+TGG 0.548880 3.2:+57120882 MS.gene049512:CDS
AAGTTTAAATACGAAAAGCT+TGG 0.550223 3.2:+57121053 MS.gene049512:CDS
GATCCGAAACCATATGATGA+AGG 0.564674 3.2:+57120611 MS.gene049512:CDS
GTCTGCGACATACAACCGAC+AGG 0.573807 3.2:-57120864 None:intergenic
AAACAACTTGGTGATTTCAT+CGG 0.577524 3.2:+57120894 MS.gene049512:CDS
ATTCTTTCATCTGAGAGACA+TGG 0.586745 3.2:+57120710 MS.gene049512:CDS
ACGATTAACTATCGCAGAAG+AGG 0.589126 3.2:+57120494 MS.gene049512:CDS
GATGAAGAAGATCAAAGTGA+AGG 0.591922 3.2:+57121013 MS.gene049512:CDS
CAAGTTCATGACGTCCCTGT+CGG 0.592554 3.2:+57120849 MS.gene049512:CDS
TCATATGGTTTCGGATCAGA+CGG 0.592721 3.2:-57120605 None:intergenic
ATGAAGAAGATCAAAGTGAA+GGG 0.604827 3.2:+57121014 MS.gene049512:CDS
ATGGTTGAGGGGAACCGTTG+TGG 0.610307 3.2:-57120811 None:intergenic
ATTATCAGACTGCTTCAGCA+AGG 0.614610 3.2:+57120780 MS.gene049512:CDS
TCCCAACCAAGCATAGGGCA+TGG 0.623148 3.2:-57120569 None:intergenic
CTCTCCTCTTATCTGCACGA+AGG 0.637531 3.2:-57121178 None:intergenic
CAATAGGGTACATGGTTGAG+GGG 0.644707 3.2:-57120822 None:intergenic
GAGTCTTAGTTGATGTACGC+AGG 0.651454 3.2:+57120979 MS.gene049512:CDS
CTTCGTGCAGATAAGAGGAG+AGG 0.653327 3.2:+57121179 MS.gene049512:CDS
GCAAGGCAATGTTCCCACAA+CGG 0.663768 3.2:+57120797 MS.gene049512:CDS
ATGTACGCAGGCCATTGATG+CGG 0.664135 3.2:+57120991 MS.gene049512:CDS
ACTTTCTGAGCACATACATG+AGG 0.671475 3.2:+57120952 MS.gene049512:CDS
GTATGTCCATCTAAAGACCA+AGG 0.674234 3.2:-57120753 None:intergenic
TCTCCCGTAGAAAGAAACAA+AGG 0.696480 3.2:+57121210 MS.gene049512:CDS
TCTGCGACATACAACCGACA+GGG 0.729112 3.2:-57120863 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGGAAAAGAAAAAGATT+AGG - chr3.2:57120693-57120712 None:intergenic 20.0%
!! ATTGGAAAAGAAAAAGATTA+GGG - chr3.2:57120692-57120711 None:intergenic 20.0%
! AAGTTTAAATACGAAAAGCT+TGG + chr3.2:57121053-57121072 MS.gene049512:CDS 25.0%
ATATCTTCTCCTTCATCATA+TGG - chr3.2:57120623-57120642 None:intergenic 30.0%
ATGAAGAAGATCAAAGTGAA+GGG + chr3.2:57121014-57121033 MS.gene049512:CDS 30.0%
! AAACAACTTGGTGATTTCAT+CGG + chr3.2:57120894-57120913 MS.gene049512:CDS 30.0%
! AATGCCTTTGTTTCTTTCTA+CGG - chr3.2:57121217-57121236 None:intergenic 30.0%
! AGGAGAAGATATCAGAATTT+TGG + chr3.2:57120631-57120650 MS.gene049512:CDS 30.0%
ATTCTTTCATCTGAGAGACA+TGG + chr3.2:57120710-57120729 MS.gene049512:CDS 35.0%
CATCATATGGATTCGAGAAT+AGG - chr3.2:57121133-57121152 None:intergenic 35.0%
GATGAAGAAGATCAAAGTGA+AGG + chr3.2:57121013-57121032 MS.gene049512:CDS 35.0%
GGAACTTTCTGTTATTACTG+TGG + chr3.2:57121074-57121093 MS.gene049512:CDS 35.0%
TCTCCTTCATCATATGGTTT+CGG - chr3.2:57120617-57120636 None:intergenic 35.0%
TGATTTCATCGGTGAGTTTA+AGG + chr3.2:57120905-57120924 MS.gene049512:CDS 35.0%
TGTCTCTCAGATGAAAGAAT+TGG - chr3.2:57120710-57120729 None:intergenic 35.0%
TTCTCGAATCCATATGATGA+TGG + chr3.2:57121134-57121153 MS.gene049512:CDS 35.0%
! ATGCCTTTGTTTCTTTCTAC+GGG - chr3.2:57121216-57121235 None:intergenic 35.0%
! TTGATAGCCACTTTACAAAC+AGG - chr3.2:57120660-57120679 None:intergenic 35.0%
!!! ATGGAAAGAGTTTTGAAGCA+GGG + chr3.2:57120729-57120748 MS.gene049512:CDS 35.0%
ACGATTAACTATCGCAGAAG+AGG + chr3.2:57120494-57120513 MS.gene049512:CDS 40.0%
ACTTTCTGAGCACATACATG+AGG + chr3.2:57120952-57120971 MS.gene049512:CDS 40.0%
ATCTTCTTCATCCGCATCAA+TGG - chr3.2:57121005-57121024 None:intergenic 40.0%
ATTATCAGACTGCTTCAGCA+AGG + chr3.2:57120780-57120799 MS.gene049512:CDS 40.0%
GATCCGAAACCATATGATGA+AGG + chr3.2:57120611-57120630 MS.gene049512:CDS 40.0%
GTATGTCCATCTAAAGACCA+AGG - chr3.2:57120756-57120775 None:intergenic 40.0%
TCATATGGTTTCGGATCAGA+CGG - chr3.2:57120608-57120627 None:intergenic 40.0%
TCATGAACTTGCACCCAATA+GGG - chr3.2:57120840-57120859 None:intergenic 40.0%
TCTCCCGTAGAAAGAAACAA+AGG + chr3.2:57121210-57121229 MS.gene049512:CDS 40.0%
TGTTATTACTGTGGCATGCT+CGG + chr3.2:57121083-57121102 MS.gene049512:CDS 40.0%
TTGGCAACCTGTTTGTAAAG+TGG + chr3.2:57120650-57120669 MS.gene049512:CDS 40.0%
! ATAGGGCATGGTTTTCTTCT+TGG - chr3.2:57120560-57120579 None:intergenic 40.0%
! TGGTTTTCTTCTTGGTTTCC+TGG - chr3.2:57120552-57120571 None:intergenic 40.0%
! TTTGTTTCTTTCTACGGGAG+AGG - chr3.2:57121211-57121230 None:intergenic 40.0%
!! ATCCATATGATGATGGAGTC+CGG + chr3.2:57121141-57121160 MS.gene049512:CDS 40.0%
!! ATTACGATTGTTGACTCTCC+AGG + chr3.2:57120531-57120550 MS.gene049512:CDS 40.0%
!!! CATGGAAAGAGTTTTGAAGC+AGG + chr3.2:57120728-57120747 MS.gene049512:CDS 40.0%
AACCGGACTCCATCATCATA+TGG - chr3.2:57121146-57121165 None:intergenic 45.0%
AGAAAACCATGCCCTATGCT+TGG + chr3.2:57120563-57120582 MS.gene049512:CDS 45.0%
AGAATCTCCCAACCAAGCAT+AGG - chr3.2:57120578-57120597 None:intergenic 45.0%
CAATAGGGTACATGGTTGAG+GGG - chr3.2:57120825-57120844 None:intergenic 45.0%
CAGACTGCAGGAAAACAACT+TGG + chr3.2:57120882-57120901 MS.gene049512:CDS 45.0%
CCAATAGGGTACATGGTTGA+GGG - chr3.2:57120826-57120845 None:intergenic 45.0%
CCTCAACCATGTACCCTATT+GGG + chr3.2:57120824-57120843 MS.gene049512:CDS 45.0%
CTTGGTTGGGAGATTCTTGA+CGG + chr3.2:57120581-57120600 MS.gene049512:CDS 45.0%
GAATCTCCCAACCAAGCATA+GGG - chr3.2:57120577-57120596 None:intergenic 45.0%
GTCATGAACTTGCACCCAAT+AGG - chr3.2:57120841-57120860 None:intergenic 45.0%
! GAGTCTTAGTTGATGTACGC+AGG + chr3.2:57120979-57120998 MS.gene049512:CDS 45.0%
!! ATGATGATGGAGTCCGGTTA+TGG + chr3.2:57121147-57121166 MS.gene049512:CDS 45.0%
AACCATGCCCTATGCTTGGT+TGG + chr3.2:57120567-57120586 MS.gene049512:CDS 50.0%
ACCATGCCCTATGCTTGGTT+GGG + chr3.2:57120568-57120587 MS.gene049512:CDS 50.0%
ATGTACGCAGGCCATTGATG+CGG + chr3.2:57120991-57121010 MS.gene049512:CDS 50.0%
CAAGTTCATGACGTCCCTGT+CGG + chr3.2:57120849-57120868 MS.gene049512:CDS 50.0%
CAGACCTTCGTGCAGATAAG+AGG + chr3.2:57121174-57121193 MS.gene049512:CDS 50.0%
CCCAATAGGGTACATGGTTG+AGG - chr3.2:57120827-57120846 None:intergenic 50.0%
CCCTCAACCATGTACCCTAT+TGG + chr3.2:57120823-57120842 MS.gene049512:CDS 50.0%
CTCTCCTCTTATCTGCACGA+AGG - chr3.2:57121181-57121200 None:intergenic 50.0%
CTTCGTGCAGATAAGAGGAG+AGG + chr3.2:57121179-57121198 MS.gene049512:CDS 50.0%
CTTGCACCCAATAGGGTACA+TGG - chr3.2:57120833-57120852 None:intergenic 50.0%
GAAGGTCTGAGCTCCATAAC+CGG - chr3.2:57121163-57121182 None:intergenic 50.0%
GCAAGGCAATGTTCCCACAA+CGG + chr3.2:57120797-57120816 MS.gene049512:CDS 50.0%
TCTGCGACATACAACCGACA+GGG - chr3.2:57120866-57120885 None:intergenic 50.0%
! CAGGGTCCTTGGTCTTTAGA+TGG + chr3.2:57120747-57120766 MS.gene049512:CDS 50.0%
!!! GAGTTTTGAAGCAGGGTCCT+TGG + chr3.2:57120736-57120755 MS.gene049512:CDS 50.0%
ATGGTTGAGGGGAACCGTTG+TGG - chr3.2:57120814-57120833 None:intergenic 55.0%
GGTTGTATGTCGCAGACTGC+AGG + chr3.2:57120870-57120889 MS.gene049512:CDS 55.0%
GTCTGCGACATACAACCGAC+AGG - chr3.2:57120867-57120886 None:intergenic 55.0%
TCCCAACCAAGCATAGGGCA+TGG - chr3.2:57120572-57120591 None:intergenic 55.0%
TGGTTGAGGGGAACCGTTGT+GGG - chr3.2:57120813-57120832 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 57120489 57121238 57120489 ID=MS.gene049512
chr3.2 mRNA 57120489 57121238 57120489 ID=MS.gene049512.t1;Parent=MS.gene049512
chr3.2 exon 57120489 57121238 57120489 ID=MS.gene049512.t1.exon1;Parent=MS.gene049512.t1
chr3.2 CDS 57120489 57121238 57120489 ID=cds.MS.gene049512.t1;Parent=MS.gene049512.t1
Gene Sequence

>MS.gene049512

ATGGAACGATTAACTATCGCAGAAGAGGAAGACGAAATCGAGATTACGATTGTTGACTCTCCAGGAAACCAAGAAGAAAACCATGCCCTATGCTTGGTTGGGAGATTCTTGACGGACCGTCTGATCCGAAACCATATGATGAAGGAGAAGATATCAGAATTTTGGCAACCTGTTTGTAAAGTGGCTATCAAAGAGATTAACCCTAATCTTTTTCTTTTCCAATTCTTTCATCTGAGAGACATGGAAAGAGTTTTGAAGCAGGGTCCTTGGTCTTTAGATGGACATACTTTGATTATCAGACTGCTTCAGCAAGGCAATGTTCCCACAACGGTTCCCCTCAACCATGTACCCTATTGGGTGCAAGTTCATGACGTCCCTGTCGGTTGTATGTCGCAGACTGCAGGAAAACAACTTGGTGATTTCATCGGTGAGTTTAAGGAATATGATGAAAAAAATAACTCTAACTTTCTGAGCACATACATGAGGATACGAGTCTTAGTTGATGTACGCAGGCCATTGATGCGGATGAAGAAGATCAAAGTGAAGGGAAACTCTCACGAAGTCAAGTTTAAATACGAAAAGCTTGGAACTTTCTGTTATTACTGTGGCATGCTCGGACACATAGAAGATTTCTGCAAGAGCCTATTCTCGAATCCATATGATGATGGAGTCCGGTTATGGAGCTCAGACCTTCGTGCAGATAAGAGGAGAGGAGCAGACCTCTCCCGTAGAAAGAAACAAAGGCATTAA

Protein sequence

>MS.gene049512.t1

MERLTIAEEEDEIEITIVDSPGNQEENHALCLVGRFLTDRLIRNHMMKEKISEFWQPVCKVAIKEINPNLFLFQFFHLRDMERVLKQGPWSLDGHTLIIRLLQQGNVPTTVPLNHVPYWVQVHDVPVGCMSQTAGKQLGDFIGEFKEYDEKNNSNFLSTYMRIRVLVDVRRPLMRMKKIKVKGNSHEVKFKYEKLGTFCYYCGMLGHIEDFCKSLFSNPYDDGVRLWSSDLRADKRRGADLSRRKKQRH