AlfalfaGEDB | MS.gene43924

M. sativa cultivar XinJiangDaYe / MS.gene43924

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43924.t1	XP_024639426.1	86.7	285	38	0	65	349	1	285	9.70E-148	533.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43924.t1	O04244	24.1	137	102	2	79	215	7	141	3.6e-10	67.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43924.t1	A0A396HU84	86.7	279	37	0	71	349	1	279	7.2e-145	523.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene43924	MS.gene61499	PPI
MS.gene09589	MS.gene43924	PPI
MS.gene43924	MS.gene019165	PPI
MS.gene43924	MS.gene81194	PPI
MS.gene43393	MS.gene43924	PPI
MS.gene34197	MS.gene43924	PPI
MS.gene43924	MS.gene038394	PPI
MS.gene43924	MS.gene019001	PPI
MS.gene005120	MS.gene43924	PPI
MS.gene43924	MS.gene53904	PPI
MS.gene43924	MS.gene73261	PPI
MS.gene048072	MS.gene43924	PPI
MS.gene060386	MS.gene43924	PPI
MS.gene71588	MS.gene43924	PPI
MS.gene43924	MS.gene85538	PPI
MS.gene43924	MS.gene025675	PPI
MS.gene04749	MS.gene43924	PPI
MS.gene43924	MS.gene055546	PPI
MS.gene05436	MS.gene43924	PPI
MS.gene43924	MS.gene71637	PPI
MS.gene43924	MS.gene55209	PPI
MS.gene059736	MS.gene43924	PPI
MS.gene70932	MS.gene43924	PPI
MS.gene35175	MS.gene43924	PPI
MS.gene43924	MS.gene28718	PPI
MS.gene43924	MS.gene022929	PPI
MS.gene021965	MS.gene43924	PPI
MS.gene055683	MS.gene43924	PPI
MS.gene042614	MS.gene43924	PPI
MS.gene021555	MS.gene43924	PPI
MS.gene43924	MS.gene068629	PPI
MS.gene43924	MS.gene009781	PPI
MS.gene96109	MS.gene43924	PPI
MS.gene43924	MS.gene048342	PPI
MS.gene43924	MS.gene069624	PPI
MS.gene43924	MS.gene21066	PPI
MS.gene009121	MS.gene43924	PPI
MS.gene43924	MS.gene013890	PPI
MS.gene94654	MS.gene43924	PPI
MS.gene43924	MS.gene98127	PPI
MS.gene43924	MS.gene073582	PPI
MS.gene36069	MS.gene43924	PPI
MS.gene43924	MS.gene028714	PPI
MS.gene43924	MS.gene048706	PPI
MS.gene61452	MS.gene43924	PPI
MS.gene43924	MS.gene69586	PPI
MS.gene83772	MS.gene43924	PPI
MS.gene43924	MS.gene45083	PPI
MS.gene43924	MS.gene049561	PPI
MS.gene43924	MS.gene008658	PPI
MS.gene008927	MS.gene43924	PPI
MS.gene016102	MS.gene43924	PPI
MS.gene46941	MS.gene43924	PPI
MS.gene045614	MS.gene43924	PPI
MS.gene96541	MS.gene43924	PPI
MS.gene43924	MS.gene80362	PPI
MS.gene43924	MS.gene49057	PPI
MS.gene000634	MS.gene43924	PPI
MS.gene43924	MS.gene03936	PPI
MS.gene63762	MS.gene43924	PPI
MS.gene43924	MS.gene048088	PPI
MS.gene30208	MS.gene43924	PPI
MS.gene025873	MS.gene43924	PPI
MS.gene012019	MS.gene43924	PPI
MS.gene013676	MS.gene43924	PPI
MS.gene05358	MS.gene43924	PPI
MS.gene43924	MS.gene86275	PPI
MS.gene43924	MS.gene034643	PPI
MS.gene95561	MS.gene43924	PPI
MS.gene018196	MS.gene43924	PPI
MS.gene065627	MS.gene43924	PPI
MS.gene57019	MS.gene43924	PPI
MS.gene43924	MS.gene012889	PPI
MS.gene018745	MS.gene43924	PPI
MS.gene65546	MS.gene43924	PPI
MS.gene43924	MS.gene038757	PPI
MS.gene43924	MS.gene000506	PPI
MS.gene03936	MS.gene43924	PPI
MS.gene038098	MS.gene43924	PPI
MS.gene43924	MS.gene028716	PPI
MS.gene43924	MS.gene74639	PPI
MS.gene43924	MS.gene60266	PPI
MS.gene43924	MS.gene51804	PPI
MS.gene60059	MS.gene43924	PPI
MS.gene43924	MS.gene013644	PPI
MS.gene43924	MS.gene54051	PPI
MS.gene43924	MS.gene22111	PPI
MS.gene28545	MS.gene43924	PPI
MS.gene43924	MS.gene049610	PPI
MS.gene28718	MS.gene43924	PPI
MS.gene43924	MS.gene37955	PPI
MS.gene008275	MS.gene43924	PPI
MS.gene43924	MS.gene010890	PPI
MS.gene038757	MS.gene43924	PPI
MS.gene064966	MS.gene43924	PPI
MS.gene43924	MS.gene061579	PPI
MS.gene43924	MS.gene04970	PPI
MS.gene43924	MS.gene060788	PPI
MS.gene046089	MS.gene43924	PPI
MS.gene047954	MS.gene43924	PPI
MS.gene43924	MS.gene41643	PPI
MS.gene43924	MS.gene77990	PPI
MS.gene036423	MS.gene43924	PPI
MS.gene43924	MS.gene062511	PPI
MS.gene43924	MS.gene027994	PPI
MS.gene43924	MS.gene26433	PPI
MS.gene43924	MS.gene007022	PPI
MS.gene43924	MS.gene69584	PPI
MS.gene72520	MS.gene43924	PPI
MS.gene43924	MS.gene52349	PPI
MS.gene66651	MS.gene43924	PPI
MS.gene43924	MS.gene021647	PPI
MS.gene43924	MS.gene76420	PPI
MS.gene43924	MS.gene46353	PPI
MS.gene35202	MS.gene43924	PPI
MS.gene051449	MS.gene43924	PPI
MS.gene63743	MS.gene43924	PPI
MS.gene43924	MS.gene61651	PPI
MS.gene48477	MS.gene43924	PPI
MS.gene43924	MS.gene016719	PPI
MS.gene30136	MS.gene43924	PPI
MS.gene43924	MS.gene88393	PPI
MS.gene43924	MS.gene054508	PPI
MS.gene008861	MS.gene43924	PPI
MS.gene43924	MS.gene053373	PPI
MS.gene012748	MS.gene43924	PPI
MS.gene83979	MS.gene43924	PPI
MS.gene43924	MS.gene041264	PPI
MS.gene43924	MS.gene63150	PPI
MS.gene85295	MS.gene43924	PPI
MS.gene47792	MS.gene43924	PPI
MS.gene46948	MS.gene43924	PPI
MS.gene43924	MS.gene072852	PPI
MS.gene98471	MS.gene43924	PPI
MS.gene43924	MS.gene65474	PPI
MS.gene43924	MS.gene025401	PPI
MS.gene43924	MS.gene08708	PPI
MS.gene43924	MS.gene06164	PPI
MS.gene43924	MS.gene50392	PPI
MS.gene43924	MS.gene22730	PPI
MS.gene93396	MS.gene43924	PPI
MS.gene43924	MS.gene032299	PPI
MS.gene43924	MS.gene017586	PPI
MS.gene05231	MS.gene43924	PPI
MS.gene43924	MS.gene037634	PPI
MS.gene43924	MS.gene057828	PPI
MS.gene43924	MS.gene08606	PPI
MS.gene010656	MS.gene43924	PPI
MS.gene43924	MS.gene000640	PPI
MS.gene000629	MS.gene43924	PPI
MS.gene041264	MS.gene43924	PPI
MS.gene41979	MS.gene43924	PPI
MS.gene43924	MS.gene98756	PPI
MS.gene64130	MS.gene43924	PPI
MS.gene05319	MS.gene43924	PPI
MS.gene56991	MS.gene43924	PPI
MS.gene050679	MS.gene43924	PPI
MS.gene43924	MS.gene071133	PPI
MS.gene43924	MS.gene98467	PPI
MS.gene43924	MS.gene62249	PPI
MS.gene43924	MS.gene024522	PPI
MS.gene20246	MS.gene43924	PPI
MS.gene055223	MS.gene43924	PPI
MS.gene43924	MS.gene010290	PPI
MS.gene053747	MS.gene43924	PPI
MS.gene43924	MS.gene92517	PPI
MS.gene43924	MS.gene36069	PPI
MS.gene65471	MS.gene43924	PPI
MS.gene43924	MS.gene045875	PPI
MS.gene43924	MS.gene84790	PPI
MS.gene061850	MS.gene43924	PPI
MS.gene43924	MS.gene044729	PPI
MS.gene26433	MS.gene43924	PPI
MS.gene43924	MS.gene60390	PPI
MS.gene43924	MS.gene039692	PPI
MS.gene43924	MS.gene55682	PPI
MS.gene43924	MS.gene002590	PPI
MS.gene042559	MS.gene43924	PPI
MS.gene98616	MS.gene43924	PPI
MS.gene042027	MS.gene43924	PPI
MS.gene85308	MS.gene43924	PPI
MS.gene69490	MS.gene43924	PPI
MS.gene43924	MS.gene059269	PPI
MS.gene015248	MS.gene43924	PPI
MS.gene43924	MS.gene057934	PPI
MS.gene43924	MS.gene024841	PPI
MS.gene80362	MS.gene43924	PPI
MS.gene43924	MS.gene069786	PPI
MS.gene44083	MS.gene43924	PPI
MS.gene013193	MS.gene43924	PPI
MS.gene04970	MS.gene43924	PPI
MS.gene08106	MS.gene43924	PPI
MS.gene43924	MS.gene025158	PPI
MS.gene43924	MS.gene40828	PPI
MS.gene58419	MS.gene43924	PPI
MS.gene43924	MS.gene51799	PPI
MS.gene43924	MS.gene98471	PPI
MS.gene43924	MS.gene29406	PPI
MS.gene23521	MS.gene43924	PPI
MS.gene29406	MS.gene43924	PPI
MS.gene43924	MS.gene40469	PPI
MS.gene43924	MS.gene09023	PPI
MS.gene43924	MS.gene025987	PPI
MS.gene87529	MS.gene43924	PPI
MS.gene024405	MS.gene43924	PPI
MS.gene43924	MS.gene95960	PPI
MS.gene43924	MS.gene043140	PPI
MS.gene04134	MS.gene43924	PPI
MS.gene062067	MS.gene43924	PPI
MS.gene43924	MS.gene03149	PPI
MS.gene57044	MS.gene43924	PPI
MS.gene43924	MS.gene29321	PPI
MS.gene038676	MS.gene43924	PPI
MS.gene43924	MS.gene015093	PPI
MS.gene71637	MS.gene43924	PPI
MS.gene042651	MS.gene43924	PPI
MS.gene43924	MS.gene24278	PPI
MS.gene43924	MS.gene000379	PPI
MS.gene43924	MS.gene34618	PPI
MS.gene05576	MS.gene43924	PPI
MS.gene43924	MS.gene59460	PPI
MS.gene43924	MS.gene060773	PPI
MS.gene43924	MS.gene66650	PPI
MS.gene43924	MS.gene08713	PPI
MS.gene034643	MS.gene43924	PPI
MS.gene43924	MS.gene09517	PPI
MS.gene43924	MS.gene87845	PPI
MS.gene43924	MS.gene24070	PPI
MS.gene43924	MS.gene58419	PPI
MS.gene43924	MS.gene26844	PPI
MS.gene43924	MS.gene64178	PPI
MS.gene43924	MS.gene074257	PPI
MS.gene071784	MS.gene43924	PPI
MS.gene43924	MS.gene073573	PPI
MS.gene061579	MS.gene43924	PPI
MS.gene43924	MS.gene44144	PPI
MS.gene76420	MS.gene43924	PPI
MS.gene071320	MS.gene43924	PPI
MS.gene43924	MS.gene05576	PPI
MS.gene42903	MS.gene43924	PPI
MS.gene43924	MS.gene060718	PPI
MS.gene81712	MS.gene43924	PPI
MS.gene026680	MS.gene43924	PPI
MS.gene43924	MS.gene48477	PPI
MS.gene43924	MS.gene027169	PPI
MS.gene048747	MS.gene43924	PPI
MS.gene43924	MS.gene004417	PPI
MS.gene43924	MS.gene019177	PPI
MS.gene22200	MS.gene43924	PPI
MS.gene26038	MS.gene43924	PPI
MS.gene43924	MS.gene055560	PPI
MS.gene43924	MS.gene009213	PPI
MS.gene43924	MS.gene024297	PPI
MS.gene43924	MS.gene003264	PPI
MS.gene43924	MS.gene054652	PPI
MS.gene031967	MS.gene43924	PPI
MS.gene43924	MS.gene37537	PPI
MS.gene34812	MS.gene43924	PPI
MS.gene02896	MS.gene43924	PPI
MS.gene063214	MS.gene43924	PPI
MS.gene97410	MS.gene43924	PPI
MS.gene43924	MS.gene42010	PPI
MS.gene32415	MS.gene43924	PPI
MS.gene43924	MS.gene27329	PPI
MS.gene035626	MS.gene43924	PPI
MS.gene43924	MS.gene41393	PPI
MS.gene067340	MS.gene43924	PPI
MS.gene43924	MS.gene89815	PPI
MS.gene65623	MS.gene43924	PPI
MS.gene43924	MS.gene016632	PPI
MS.gene43924	MS.gene018474	PPI
MS.gene43924	MS.gene86868	PPI
MS.gene71675	MS.gene43924	PPI
MS.gene69495	MS.gene43924	PPI
MS.gene049512	MS.gene43924	PPI
MS.gene43924	MS.gene058889	PPI
MS.gene038623	MS.gene43924	PPI
MS.gene43924	MS.gene96163	PPI
MS.gene05357	MS.gene43924	PPI
MS.gene032311	MS.gene43924	PPI
MS.gene030090	MS.gene43924	PPI
MS.gene96174	MS.gene43924	PPI
MS.gene86849	MS.gene43924	PPI
MS.gene43924	MS.gene34463	PPI
MS.gene39956	MS.gene43924	PPI
MS.gene43924	MS.gene037049	PPI
MS.gene021968	MS.gene43924	PPI
MS.gene43924	MS.gene026680	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43924.t1	MTR_6g033215	44.186	172	96	0	25	196	123	294	8.56e-53	176
MS.gene43924.t1	MTR_0010s0370	36.538	156	99	0	19	174	22	177	3.00e-35	127
MS.gene43924.t1	MTR_0010s0350	36.538	156	99	0	19	174	22	177	3.00e-35	127
MS.gene43924.t1	MTR_6g053230	33.708	178	80	2	6	183	30	169	1.82e-23	101
MS.gene43924.t1	MTR_3g053920	32.450	151	85	2	14	164	19	152	3.38e-23	97.8
MS.gene43924.t1	MTR_8g468010	57.812	64	27	0	112	175	1	64	5.86e-21	88.2
MS.gene43924.t1	MTR_2g061500	33.333	111	74	0	14	124	19	129	4.23e-20	85.5
MS.gene43924.t1	MTR_3g024240	36.508	126	78	1	74	197	50	175	8.43e-20	86.3
MS.gene43924.t1	MTR_1g032270	35.652	115	67	3	96	209	2	110	1.11e-16	76.6
MS.gene43924.t1	MTR_1g036720	36.264	91	51	1	24	107	20	110	2.72e-14	68.9
MS.gene43924.t1	MTR_4g103530	27.869	122	85	1	50	168	1	122	7.20e-14	69.7
MS.gene43924.t1	MTR_4g020545	27.551	196	90	6	56	251	77	220	1.54e-13	70.5
MS.gene43924.t1	MTR_7g089990	30.000	130	80	2	101	228	86	206	2.53e-12	65.9
MS.gene43924.t1	MTR_6g053790	26.506	166	92	5	132	292	34	174	2.70e-12	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43924.t1	AT3G57587	25.000	148	103	3	70	215	80	221	6.13e-13	69.7
MS.gene43924.t1	AT3G31430	23.497	183	136	1	29	211	132	310	6.84e-13	69.3
MS.gene43924.t1	AT2G17920	20.541	185	145	1	31	215	39	221	1.53e-12	67.8
MS.gene43924.t1	AT2G13450	19.890	181	143	2	31	211	39	217	1.66e-12	68.6
MS.gene43924.t1	AT2G41590	21.081	185	144	1	31	215	39	221	3.34e-12	67.4
MS.gene43924.t1	AT4G02000	24.088	137	102	2	79	215	7	141	4.18e-12	66.6
MS.gene43924.t1	AT5G18636	19.681	188	149	1	28	215	36	221	1.02e-11	65.9
MS.gene43924.t1	AT5G25200	19.681	188	149	1	28	215	36	221	1.05e-11	65.9
MS.gene43924.t1	AT2G02103	19.681	188	149	1	28	215	36	221	1.26e-11	65.9

Find 100 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGTTTCATGACTGAGACTA+TGG	0.192322	5.4:+25271276	MS.gene43924:CDS
CCATAATTCAATTGAGTCTA+TGG	0.207359	5.4:-25271230	None:intergenic
CCATAGACTCAATTGAATTA+TGG	0.209859	5.4:+25271230	MS.gene43924:CDS
GCTCTTTAACTATGCGGATA+TGG	0.248796	5.4:+25271117	MS.gene43924:CDS
TATGATTATGAGAATAACTA+TGG	0.254756	5.4:+25271337	MS.gene43924:CDS
ACTGCTGTTCAGAATTGTTT+TGG	0.297247	5.4:-25271860	None:intergenic
TGAAACTGAAGGGGAGATTA+TGG	0.318136	5.4:-25271261	None:intergenic
AGCGGACAGGTTTGGTTGTT+AGG	0.320646	5.4:-25271003	None:intergenic
AGCTGAACTTCATGTGTTGC+AGG	0.341306	5.4:-25271898	None:intergenic
GCATTCATAGCAGAAATTTC+CGG	0.345427	5.4:-25271482	None:intergenic
TTCTTCAATGGTTCTTTAAC+CGG	0.347583	5.4:-25271397	None:intergenic
CATTCATAGCAGAAATTTCC+GGG	0.350770	5.4:-25271481	None:intergenic
GGAATAGAAGCAGGAGAACT+TGG	0.355492	5.4:+25271653	MS.gene43924:CDS
TGCTATGAATGCGGTTTGAT+AGG	0.359866	5.4:+25271493	MS.gene43924:CDS
AATTCAAAACTCTTCTTCAA+TGG	0.361102	5.4:-25271409	None:intergenic
TTGATAGCTACCCTCTAATC+TGG	0.363161	5.4:+25271170	MS.gene43924:CDS
CATAGACTCAATTGAATTAT+GGG	0.364409	5.4:+25271231	MS.gene43924:CDS
TATCTGAGGTCTGATTATAT+TGG	0.383266	5.4:+25271586	MS.gene43924:CDS
AGCTGAAACATGAAACAGTT+TGG	0.387762	5.4:-25271097	None:intergenic
CCCATTCGTTAGAGTCGTAT+AGG	0.397181	5.4:-25271780	None:intergenic
TCAGGTTGATATTACCTTGA+TGG	0.399790	5.4:+25271072	MS.gene43924:CDS
GTAACCTTGCTCCAGATTAG+AGG	0.404545	5.4:-25271181	None:intergenic
GACACAGGATGCTGCTCAAC+CGG	0.409211	5.4:+25271831	MS.gene43924:CDS
TGGAGCACGGTGCTGCTGTA+AGG	0.410833	5.4:+25271437	MS.gene43924:CDS
AAACCTTTCTCAAAACGCTT+AGG	0.424425	5.4:-25271535	None:intergenic
TCTAACTTACATCGTGTGTT+TGG	0.447121	5.4:+25271694	MS.gene43924:CDS
TGTGGAAGGACAACAACAAT+GGG	0.451209	5.4:+25271558	MS.gene43924:CDS
AGACAATAGTCAAGCGTGTC+AGG	0.451214	5.4:-25270968	None:intergenic
AAACCCGTATGTCAACCTTA+TGG	0.455641	5.4:+25271038	MS.gene43924:CDS
CGCTTTAATGTTGAAAGTGT+TGG	0.461894	5.4:+25271805	MS.gene43924:CDS
TGAGACTATGGGAATTCTGC+TGG	0.464200	5.4:+25271288	MS.gene43924:CDS
CCATTCGTTAGAGTCGTATA+GGG	0.465751	5.4:-25271779	None:intergenic
ATGAGAATAACTATGGGAAC+TGG	0.467764	5.4:+25271344	MS.gene43924:CDS
GAGGCTGAGAGTATCTTTGC+CGG	0.467860	5.4:+25271378	MS.gene43924:CDS
TTCTCGACCATTCCTCCCTC+AGG	0.471977	5.4:-25271610	None:intergenic
GTGTTTGGTAGAGTTAGAAT+TGG	0.472757	5.4:+25271709	MS.gene43924:CDS
AACCTTTCTCAAAACGCTTA+GGG	0.480351	5.4:-25271534	None:intergenic
CTGCCATAAGGTTGACATAC+GGG	0.488070	5.4:-25271041	None:intergenic
CAGGAGTGTAGGGTGTTAGA+TGG	0.488427	5.4:-25270949	None:intergenic
ACGCTTGACTATTGTCTTGT+GGG	0.495629	5.4:+25270974	MS.gene43924:CDS
CCTATACGACTCTAACGAAT+GGG	0.497537	5.4:+25271780	MS.gene43924:CDS
CTTATGGCAGCCTAAGCATC+AGG	0.502653	5.4:+25271054	MS.gene43924:CDS
TTGAAGTTGTGGAAGCGGAC+AGG	0.503354	5.4:-25271016	None:intergenic
TAGGGCCGGAGCCATCTGTA+TGG	0.514963	5.4:-25271516	None:intergenic
GAAACAGTTTGGTTCCATCA+AGG	0.519240	5.4:-25271086	None:intergenic
TGCAACACATGAAGTTCAGC+TGG	0.522216	5.4:+25271900	MS.gene43924:CDS
CACGCTTGACTATTGTCTTG+TGG	0.525579	5.4:+25270973	MS.gene43924:CDS
GTCAAGCGTGTCAGGAGTGT+AGG	0.527405	5.4:-25270960	None:intergenic
ATATAATCAGACCTCAGATA+TGG	0.527968	5.4:-25271583	None:intergenic
CCCTATACGACTCTAACGAA+TGG	0.531770	5.4:+25271779	MS.gene43924:CDS
ACATGAAGTTCAGCTGGTAG+AGG	0.533325	5.4:+25271906	MS.gene43924:CDS
GTAATATCAACCTGATGCTT+AGG	0.533996	5.4:-25271064	None:intergenic
CTGATGCTTAGGCTGCCATA+AGG	0.535818	5.4:-25271053	None:intergenic
GTTGTGGAAGCGGACAGGTT+TGG	0.537125	5.4:-25271011	None:intergenic
TTTCTCAAAACGCTTAGGGC+CGG	0.544684	5.4:-25271530	None:intergenic
CATAATTCAATTGAGTCTAT+GGG	0.549652	5.4:-25271229	None:intergenic
ATTCTGCTGGGCAATCATGT+TGG	0.553807	5.4:+25271301	MS.gene43924:CDS
TCAAGCGTGTCAGGAGTGTA+GGG	0.554658	5.4:-25270959	None:intergenic
GTCTCAGTCATGAAACTGAA+GGG	0.563383	5.4:-25271271	None:intergenic
ATAGGCCATACAGATGGCTC+CGG	0.567629	5.4:+25271511	MS.gene43924:CDS
AGTTTCATGACTGAGACTAT+GGG	0.570302	5.4:+25271277	MS.gene43924:CDS
GTCTGATTATATTGGACCTG+AGG	0.571944	5.4:+25271594	MS.gene43924:CDS
TTGTGGAAGGACAACAACAA+TGG	0.573417	5.4:+25271557	MS.gene43924:CDS
TTTGAATTTGTGTTGGAGCA+CGG	0.573610	5.4:+25271424	MS.gene43924:CDS
TATTGGACCTGAGGGAGGAA+TGG	0.578406	5.4:+25271603	MS.gene43924:CDS
ATCTGGAGCAAGGTTACTGA+TGG	0.580911	5.4:+25271187	MS.gene43924:CDS
GGAAGAGATGCGGTGACTAG+AGG	0.583447	5.4:+25271730	MS.gene43924:CDS
AATTTCCGGGTTTCTCGTAA+CGG	0.585742	5.4:-25271468	None:intergenic
AGTTAGAATTGGAAGAGATG+CGG	0.586735	5.4:+25271720	MS.gene43924:CDS
TAACCTTGCTCCAGATTAGA+GGG	0.589023	5.4:-25271180	None:intergenic
TTCCTACCATCATGCAGCCA+CGG	0.594167	5.4:-25271634	None:intergenic
CATGAAGTTCAGCTGGTAGA+GGG	0.595313	5.4:+25271907	MS.gene43924:CDS
GAGTCTATGGGCATAGTGAA+CGG	0.596705	5.4:-25271217	None:intergenic
TCAGCTGGTAGAGGGAGAAG+AGG	0.597236	5.4:+25271915	MS.gene43924:CDS
GAGACTATGGGAATTCTGCT+GGG	0.598946	5.4:+25271289	MS.gene43924:CDS
GGAGCACGGTGCTGCTGTAA+GGG	0.600608	5.4:+25271438	MS.gene43924:CDS
GAGAATCCGTGGCTGCATGA+TGG	0.600897	5.4:+25271628	MS.gene43924:CDS
CAGGATGCTGCTCAACCGGT+TGG	0.601005	5.4:+25271835	MS.gene43924:CDS
ATGATTATGAGAATAACTAT+GGG	0.604034	5.4:+25271338	MS.gene43924:CDS
GAATAGAAGCAGGAGAACTT+GGG	0.605196	5.4:+25271654	MS.gene43924:CDS
GGAAATTTCTGCTATGAATG+CGG	0.609274	5.4:+25271484	MS.gene43924:CDS
TCTGATTATATTGGACCTGA+GGG	0.609681	5.4:+25271595	MS.gene43924:CDS
GAGGAATGGTCGAGAATCCG+TGG	0.623399	5.4:+25271617	MS.gene43924:CDS
GTTTCAGCTCTTTAACTATG+CGG	0.626694	5.4:+25271111	MS.gene43924:CDS
ATGATGGTAGGAATAGAAGC+AGG	0.635126	5.4:+25271644	MS.gene43924:CDS
GAGTCATACAACAAGGACCT+TGG	0.637685	5.4:+25271143	MS.gene43924:CDS
ATCCGTGGCTGCATGATGGT+AGG	0.638796	5.4:+25271632	MS.gene43924:CDS
AGTCTCAGTCATGAAACTGA+AGG	0.639247	5.4:-25271272	None:intergenic
CTACCCTCTAATCTGGAGCA+AGG	0.640542	5.4:+25271177	MS.gene43924:CDS
GCTGCCATAAGGTTGACATA+CGG	0.642553	5.4:-25271042	None:intergenic
GATTATATTGGACCTGAGGG+AGG	0.645153	5.4:+25271598	MS.gene43924:CDS
TCTCAGTCATGAAACTGAAG+GGG	0.648523	5.4:-25271270	None:intergenic
AACAATGGGGACCATATCTG+AGG	0.669044	5.4:+25271572	MS.gene43924:CDS
AACTTCCGTTACGAGAAACC+CGG	0.676987	5.4:+25271463	MS.gene43924:CDS
GGTTTGATAGGCCATACAGA+TGG	0.679566	5.4:+25271505	MS.gene43924:CDS
TGAAAGTGTTGGTCAGACAC+AGG	0.682545	5.4:+25271816	MS.gene43924:CDS
GGAACTGGAGAAGATACATG+AGG	0.718382	5.4:+25271359	MS.gene43924:CDS
GGGTAGCTATCAAACAACCA+AGG	0.725612	5.4:-25271160	None:intergenic
ATGGAACGAGTCATACAACA+AGG	0.729061	5.4:+25271136	MS.gene43924:CDS
GTGGAAGGACAACAACAATG+GGG	0.750352	5.4:+25271559	MS.gene43924:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGATTATGAGAATAACTAT+GGG	+	chr5.4:25271338-25271357	MS.gene43924:CDS	20.0%
!!	TATGATTATGAGAATAACTA+TGG	+	chr5.4:25271337-25271356	MS.gene43924:CDS	20.0%
!	AATTCAAAACTCTTCTTCAA+TGG	-	chr5.4:25271412-25271431	None:intergenic	25.0%
!	CATAATTCAATTGAGTCTAT+GGG	-	chr5.4:25271232-25271251	None:intergenic	25.0%
!	CATAGACTCAATTGAATTAT+GGG	+	chr5.4:25271231-25271250	MS.gene43924:CDS	25.0%
	ATATAATCAGACCTCAGATA+TGG	-	chr5.4:25271586-25271605	None:intergenic	30.0%
	CCATAATTCAATTGAGTCTA+TGG	-	chr5.4:25271233-25271252	None:intergenic	30.0%
	CCATAGACTCAATTGAATTA+TGG	+	chr5.4:25271230-25271249	MS.gene43924:CDS	30.0%
	TTCTTCAATGGTTCTTTAAC+CGG	-	chr5.4:25271400-25271419	None:intergenic	30.0%
!	TATCTGAGGTCTGATTATAT+TGG	+	chr5.4:25271586-25271605	MS.gene43924:CDS	30.0%
!!!	GAAGAGTTTTGAATTTGTGT+TGG	+	chr5.4:25271417-25271436	MS.gene43924:CDS	30.0%
	AAACCTTTCTCAAAACGCTT+AGG	-	chr5.4:25271538-25271557	None:intergenic	35.0%
	AACCTTTCTCAAAACGCTTA+GGG	-	chr5.4:25271537-25271556	None:intergenic	35.0%
	AGCTGAAACATGAAACAGTT+TGG	-	chr5.4:25271100-25271119	None:intergenic	35.0%
	AGTTAGAATTGGAAGAGATG+CGG	+	chr5.4:25271720-25271739	MS.gene43924:CDS	35.0%
	AGTTTCATGACTGAGACTAT+GGG	+	chr5.4:25271277-25271296	MS.gene43924:CDS	35.0%
	ATGAGAATAACTATGGGAAC+TGG	+	chr5.4:25271344-25271363	MS.gene43924:CDS	35.0%
	CATTCATAGCAGAAATTTCC+GGG	-	chr5.4:25271484-25271503	None:intergenic	35.0%
	GCATTCATAGCAGAAATTTC+CGG	-	chr5.4:25271485-25271504	None:intergenic	35.0%
	GGAAATTTCTGCTATGAATG+CGG	+	chr5.4:25271484-25271503	MS.gene43924:CDS	35.0%
	GTAATATCAACCTGATGCTT+AGG	-	chr5.4:25271067-25271086	None:intergenic	35.0%
	GTGTTTGGTAGAGTTAGAAT+TGG	+	chr5.4:25271709-25271728	MS.gene43924:CDS	35.0%
	GTTTCAGCTCTTTAACTATG+CGG	+	chr5.4:25271111-25271130	MS.gene43924:CDS	35.0%
	TCTAACTTACATCGTGTGTT+TGG	+	chr5.4:25271694-25271713	MS.gene43924:CDS	35.0%
	TCTGATTATATTGGACCTGA+GGG	+	chr5.4:25271595-25271614	MS.gene43924:CDS	35.0%
	TTTGAATTTGTGTTGGAGCA+CGG	+	chr5.4:25271424-25271443	MS.gene43924:CDS	35.0%
!	ACTGCTGTTCAGAATTGTTT+TGG	-	chr5.4:25271863-25271882	None:intergenic	35.0%
!	CGCTTTAATGTTGAAAGTGT+TGG	+	chr5.4:25271805-25271824	MS.gene43924:CDS	35.0%
!!	ATGGCTATTTTCAGTGGAAT+TGG	-	chr5.4:25270933-25270952	None:intergenic	35.0%
!!	CTAGTCTTTTACAGACACAT+TGG	+	chr5.4:25271754-25271773	MS.gene43924:CDS	35.0%
!!	TCAGGTTGATATTACCTTGA+TGG	+	chr5.4:25271072-25271091	MS.gene43924:CDS	35.0%
!!	TGTTAGATGGCTATTTTCAG+TGG	-	chr5.4:25270939-25270958	None:intergenic	35.0%
!!	TTTGAGAAAGGTTTTGTGGA+AGG	+	chr5.4:25271544-25271563	MS.gene43924:CDS	35.0%
!!!	AGAATTGTTTTGGAACCAAC+CGG	-	chr5.4:25271853-25271872	None:intergenic	35.0%
	AAACCCGTATGTCAACCTTA+TGG	+	chr5.4:25271038-25271057	MS.gene43924:CDS	40.0%
	AATTTCCGGGTTTCTCGTAA+CGG	-	chr5.4:25271471-25271490	None:intergenic	40.0%
	ACGCTTGACTATTGTCTTGT+GGG	+	chr5.4:25270974-25270993	MS.gene43924:CDS	40.0%
	AGTCTCAGTCATGAAACTGA+AGG	-	chr5.4:25271275-25271294	None:intergenic	40.0%
	ATGATGGTAGGAATAGAAGC+AGG	+	chr5.4:25271644-25271663	MS.gene43924:CDS	40.0%
	ATGGAACGAGTCATACAACA+AGG	+	chr5.4:25271136-25271155	MS.gene43924:CDS	40.0%
	CAGTTTCATGACTGAGACTA+TGG	+	chr5.4:25271276-25271295	MS.gene43924:CDS	40.0%
	CCATTCGTTAGAGTCGTATA+GGG	-	chr5.4:25271782-25271801	None:intergenic	40.0%
	CCTATACGACTCTAACGAAT+GGG	+	chr5.4:25271780-25271799	MS.gene43924:CDS	40.0%
	GAATAGAAGCAGGAGAACTT+GGG	+	chr5.4:25271654-25271673	MS.gene43924:CDS	40.0%
	GCTCTTTAACTATGCGGATA+TGG	+	chr5.4:25271117-25271136	MS.gene43924:CDS	40.0%
	GTCTCAGTCATGAAACTGAA+GGG	-	chr5.4:25271274-25271293	None:intergenic	40.0%
	GTCTGATTATATTGGACCTG+AGG	+	chr5.4:25271594-25271613	MS.gene43924:CDS	40.0%
	TAACCTTGCTCCAGATTAGA+GGG	-	chr5.4:25271183-25271202	None:intergenic	40.0%
	TCTCAGTCATGAAACTGAAG+GGG	-	chr5.4:25271273-25271292	None:intergenic	40.0%
	TGAAACTGAAGGGGAGATTA+TGG	-	chr5.4:25271264-25271283	None:intergenic	40.0%
	TGCTATGAATGCGGTTTGAT+AGG	+	chr5.4:25271493-25271512	MS.gene43924:CDS	40.0%
	TGTGGAAGGACAACAACAAT+GGG	+	chr5.4:25271558-25271577	MS.gene43924:CDS	40.0%
	TTGTGGAAGGACAACAACAA+TGG	+	chr5.4:25271557-25271576	MS.gene43924:CDS	40.0%
!	GAAACAGTTTGGTTCCATCA+AGG	-	chr5.4:25271089-25271108	None:intergenic	40.0%
!	TTGATAGCTACCCTCTAATC+TGG	+	chr5.4:25271170-25271189	MS.gene43924:CDS	40.0%
!!	GGTGTTTTGAGCAGAATTTG+AGG	-	chr5.4:25270912-25270931	None:intergenic	40.0%
!!!	ACATACGGGTTTTGAAGTTG+TGG	-	chr5.4:25271030-25271049	None:intergenic	40.0%
!!!	GCGTTTTGAGAAAGGTTTTG+TGG	+	chr5.4:25271540-25271559	MS.gene43924:CDS	40.0%
	AACAATGGGGACCATATCTG+AGG	+	chr5.4:25271572-25271591	MS.gene43924:CDS	45.0%
	AACTTCCGTTACGAGAAACC+CGG	+	chr5.4:25271463-25271482	MS.gene43924:CDS	45.0%
	ACATGAAGTTCAGCTGGTAG+AGG	+	chr5.4:25271906-25271925	MS.gene43924:CDS	45.0%
	AGACAATAGTCAAGCGTGTC+AGG	-	chr5.4:25270971-25270990	None:intergenic	45.0%
	AGCTGAACTTCATGTGTTGC+AGG	-	chr5.4:25271901-25271920	None:intergenic	45.0%
	ATTCTGCTGGGCAATCATGT+TGG	+	chr5.4:25271301-25271320	MS.gene43924:CDS	45.0%
	CACGCTTGACTATTGTCTTG+TGG	+	chr5.4:25270973-25270992	MS.gene43924:CDS	45.0%
	CATGAAGTTCAGCTGGTAGA+GGG	+	chr5.4:25271907-25271926	MS.gene43924:CDS	45.0%
	CCCATTCGTTAGAGTCGTAT+AGG	-	chr5.4:25271783-25271802	None:intergenic	45.0%
	CCCTATACGACTCTAACGAA+TGG	+	chr5.4:25271779-25271798	MS.gene43924:CDS	45.0%
	CTGCCATAAGGTTGACATAC+GGG	-	chr5.4:25271044-25271063	None:intergenic	45.0%
	GAGACTATGGGAATTCTGCT+GGG	+	chr5.4:25271289-25271308	MS.gene43924:CDS	45.0%
	GAGTCATACAACAAGGACCT+TGG	+	chr5.4:25271143-25271162	MS.gene43924:CDS	45.0%
	GAGTCTATGGGCATAGTGAA+CGG	-	chr5.4:25271220-25271239	None:intergenic	45.0%
	GATTATATTGGACCTGAGGG+AGG	+	chr5.4:25271598-25271617	MS.gene43924:CDS	45.0%
	GCTGCCATAAGGTTGACATA+CGG	-	chr5.4:25271045-25271064	None:intergenic	45.0%
	GGAACTGGAGAAGATACATG+AGG	+	chr5.4:25271359-25271378	MS.gene43924:CDS	45.0%
	GGAATAGAAGCAGGAGAACT+TGG	+	chr5.4:25271653-25271672	MS.gene43924:CDS	45.0%
	GGGTAGCTATCAAACAACCA+AGG	-	chr5.4:25271163-25271182	None:intergenic	45.0%
	GGTTTGATAGGCCATACAGA+TGG	+	chr5.4:25271505-25271524	MS.gene43924:CDS	45.0%
	GTAACCTTGCTCCAGATTAG+AGG	-	chr5.4:25271184-25271203	None:intergenic	45.0%
	GTGGAAGGACAACAACAATG+GGG	+	chr5.4:25271559-25271578	MS.gene43924:CDS	45.0%
	TGAGACTATGGGAATTCTGC+TGG	+	chr5.4:25271288-25271307	MS.gene43924:CDS	45.0%
	TGCAACACATGAAGTTCAGC+TGG	+	chr5.4:25271900-25271919	MS.gene43924:CDS	45.0%
	TTTCTCAAAACGCTTAGGGC+CGG	-	chr5.4:25271533-25271552	None:intergenic	45.0%
!	ATCTGGAGCAAGGTTACTGA+TGG	+	chr5.4:25271187-25271206	MS.gene43924:CDS	45.0%
!!	TGAAAGTGTTGGTCAGACAC+AGG	+	chr5.4:25271816-25271835	MS.gene43924:CDS	45.0%
!!!	GGGTTTTGAAGTTGTGGAAG+CGG	-	chr5.4:25271024-25271043	None:intergenic	45.0%
	ATAGGCCATACAGATGGCTC+CGG	+	chr5.4:25271511-25271530	MS.gene43924:CDS	50.0%
	CTGATGCTTAGGCTGCCATA+AGG	-	chr5.4:25271056-25271075	None:intergenic	50.0%
	CTTATGGCAGCCTAAGCATC+AGG	+	chr5.4:25271054-25271073	MS.gene43924:CDS	50.0%
	TATTGGACCTGAGGGAGGAA+TGG	+	chr5.4:25271603-25271622	MS.gene43924:CDS	50.0%
	TTCCTACCATCATGCAGCCA+CGG	-	chr5.4:25271637-25271656	None:intergenic	50.0%
	TTGAAGTTGTGGAAGCGGAC+AGG	-	chr5.4:25271019-25271038	None:intergenic	50.0%
!	AGCGGACAGGTTTGGTTGTT+AGG	-	chr5.4:25271006-25271025	None:intergenic	50.0%
!	CAGGAGTGTAGGGTGTTAGA+TGG	-	chr5.4:25270952-25270971	None:intergenic	50.0%
!	CTACCCTCTAATCTGGAGCA+AGG	+	chr5.4:25271177-25271196	MS.gene43924:CDS	50.0%
!	TCAAGCGTGTCAGGAGTGTA+GGG	-	chr5.4:25270962-25270981	None:intergenic	50.0%
!!	GAGGCTGAGAGTATCTTTGC+CGG	+	chr5.4:25271378-25271397	MS.gene43924:CDS	50.0%
!!!	GGCCCTAAGCGTTTTGAGAA+AGG	+	chr5.4:25271532-25271551	MS.gene43924:CDS	50.0%
	ATCCGTGGCTGCATGATGGT+AGG	+	chr5.4:25271632-25271651	MS.gene43924:CDS	55.0%
	GACACAGGATGCTGCTCAAC+CGG	+	chr5.4:25271831-25271850	MS.gene43924:CDS	55.0%
	GAGAATCCGTGGCTGCATGA+TGG	+	chr5.4:25271628-25271647	MS.gene43924:CDS	55.0%
	GAGGAATGGTCGAGAATCCG+TGG	+	chr5.4:25271617-25271636	MS.gene43924:CDS	55.0%
	GGAAGAGATGCGGTGACTAG+AGG	+	chr5.4:25271730-25271749	MS.gene43924:CDS	55.0%
	GTTGTGGAAGCGGACAGGTT+TGG	-	chr5.4:25271014-25271033	None:intergenic	55.0%
	TCAGCTGGTAGAGGGAGAAG+AGG	+	chr5.4:25271915-25271934	MS.gene43924:CDS	55.0%
	TTCTCGACCATTCCTCCCTC+AGG	-	chr5.4:25271613-25271632	None:intergenic	55.0%
!	GTCAAGCGTGTCAGGAGTGT+AGG	-	chr5.4:25270963-25270982	None:intergenic	55.0%
	CAGGATGCTGCTCAACCGGT+TGG	+	chr5.4:25271835-25271854	MS.gene43924:CDS	60.0%
	TAGGGCCGGAGCCATCTGTA+TGG	-	chr5.4:25271519-25271538	None:intergenic	60.0%
!!	GGAGCACGGTGCTGCTGTAA+GGG	+	chr5.4:25271438-25271457	MS.gene43924:CDS	60.0%
!!	TGGAGCACGGTGCTGCTGTA+AGG	+	chr5.4:25271437-25271456	MS.gene43924:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	25270899	25271951	25270899	ID=MS.gene43924
chr5.4	mRNA	25270899	25271951	25270899	ID=MS.gene43924.t1;Parent=MS.gene43924
chr5.4	exon	25270899	25271951	25270899	ID=MS.gene43924.t1.exon1;Parent=MS.gene43924.t1
chr5.4	CDS	25270899	25271951	25270899	ID=cds.MS.gene43924.t1;Parent=MS.gene43924.t1

Gene Sequence

>MS.gene43924

ATGTCAACATCCTCAAATTCTGCTCAAAACACCAATTCCACTGAAAATAGCCATCTAACACCCTACACTCCTGACACGCTTGACTATTGTCTTGTGGGAAGACTCCTAACAACCAAACCTGTCCGCTTCCACAACTTCAAAACCCGTATGTCAACCTTATGGCAGCCTAAGCATCAGGTTGATATTACCTTGATGGAACCAAACTGTTTCATGTTTCAGCTCTTTAACTATGCGGATATGGAACGAGTCATACAACAAGGACCTTGGTTGTTTGATAGCTACCCTCTAATCTGGAGCAAGGTTACTGATGGCAAAGATCCGTTCACTATGCCCATAGACTCAATTGAATTATGGGTGCAAGTCCATAATCTCCCCTTCAGTTTCATGACTGAGACTATGGGAATTCTGCTGGGCAATCATGTTGGAAAACTAGTAAAATATGATTATGAGAATAACTATGGGAACTGGAGAAGATACATGAGGCTGAGAGTATCTTTGCCGGTTAAAGAACCATTGAAGAAGAGTTTTGAATTTGTGTTGGAGCACGGTGCTGCTGTAAGGGTGAACTTCCGTTACGAGAAACCCGGAAATTTCTGCTATGAATGCGGTTTGATAGGCCATACAGATGGCTCCGGCCCTAAGCGTTTTGAGAAAGGTTTTGTGGAAGGACAACAACAATGGGGACCATATCTGAGGTCTGATTATATTGGACCTGAGGGAGGAATGGTCGAGAATCCGTGGCTGCATGATGGTAGGAATAGAAGCAGGAGAACTTGGGCTCGTGCTACTGAATGCTCTAACTTACATCGTGTGTTTGGTAGAGTTAGAATTGGAAGAGATGCGGTGACTAGAGGACTAGTCTTTTACAGACACATTGGTGCCCTATACGACTCTAACGAATGGGTGCGCTTTAATGTTGAAAGTGTTGGTCAGACACAGGATGCTGCTCAACCGGTTGGTTCCAAAACAATTCTGAACAGCAGTTTGCAGATTGCAGATCCTGCAACACATGAAGTTCAGCTGGTAGAGGGAGAAGAGGCGCGAATTGAGTGA

Protein sequence

>MS.gene43924.t1

MSTSSNSAQNTNSTENSHLTPYTPDTLDYCLVGRLLTTKPVRFHNFKTRMSTLWQPKHQVDITLMEPNCFMFQLFNYADMERVIQQGPWLFDSYPLIWSKVTDGKDPFTMPIDSIELWVQVHNLPFSFMTETMGILLGNHVGKLVKYDYENNYGNWRRYMRLRVSLPVKEPLKKSFEFVLEHGAAVRVNFRYEKPGNFCYECGLIGHTDGSGPKRFEKGFVEGQQQWGPYLRSDYIGPEGGMVENPWLHDGRNRSRRTWARATECSNLHRVFGRVRIGRDAVTRGLVFYRHIGALYDSNEWVRFNVESVGQTQDAAQPVGSKTILNSSLQIADPATHEVQLVEGEEARIE