AlfalfaGEDB | MS.gene010656

M. sativa cultivar XinJiangDaYe / MS.gene010656

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010656.t1	GAU48823.1	35	792	407	14	1	783	1	693	3.50E-132	482.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010656.t1	A0A2Z6P2S7	35.0	792	407	14	1	783	1	693	2.5e-132	482.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene010656	MS.gene48477	PPI
MS.gene010656	MS.gene055223	PPI
MS.gene30136	MS.gene010656	PPI
MS.gene010656	MS.gene027169	PPI
MS.gene010656	MS.gene004417	PPI
MS.gene010656	MS.gene81712	PPI
MS.gene010656	MS.gene019177	PPI
MS.gene010656	MS.gene031967	PPI
MS.gene010656	MS.gene05436	PPI
MS.gene83979	MS.gene010656	PPI
MS.gene010656	MS.gene038623	PPI
MS.gene010656	MS.gene005120	PPI
MS.gene010656	MS.gene055560	PPI
MS.gene47792	MS.gene010656	PPI
MS.gene46948	MS.gene010656	PPI
MS.gene010656	MS.gene024297	PPI
MS.gene010656	MS.gene35202	PPI
MS.gene010656	MS.gene000634	PPI
MS.gene010656	MS.gene37537	PPI
MS.gene010656	MS.gene069786	PPI
MS.gene93396	MS.gene010656	PPI
MS.gene010656	MS.gene42010	PPI
MS.gene010656	MS.gene025158	PPI
MS.gene010656	MS.gene049512	PPI
MS.gene010656	MS.gene27329	PPI
MS.gene010656	MS.gene41393	PPI
MS.gene05231	MS.gene010656	PPI
MS.gene010656	MS.gene97410	PPI
MS.gene010656	MS.gene016632	PPI
MS.gene010656	MS.gene018474	PPI
MS.gene010656	MS.gene40469	PPI
MS.gene010656	MS.gene96163	PPI
MS.gene010656	MS.gene65623	PPI
MS.gene050679	MS.gene010656	PPI
MS.gene010656	MS.gene012748	PPI
MS.gene010656	MS.gene66651	PPI
MS.gene010656	MS.gene02896	PPI
MS.gene20246	MS.gene010656	PPI
MS.gene055223	MS.gene010656	PPI
MS.gene010656	MS.gene71588	PPI
MS.gene010656	MS.gene05231	PPI
MS.gene061850	MS.gene010656	PPI
MS.gene010656	MS.gene037049	PPI
MS.gene010656	MS.gene042614	PPI
MS.gene010656	MS.gene026680	PPI
MS.gene010656	MS.gene61499	PPI
MS.gene010656	MS.gene051449	PPI
MS.gene042559	MS.gene010656	PPI
MS.gene010656	MS.gene019165	PPI
MS.gene010656	MS.gene81194	PPI
MS.gene010656	MS.gene060773	PPI
MS.gene98616	MS.gene010656	PPI
MS.gene010656	MS.gene038394	PPI
MS.gene010656	MS.gene09589	PPI
MS.gene69490	MS.gene010656	PPI
MS.gene010656	MS.gene019001	PPI
MS.gene010656	MS.gene021965	PPI
MS.gene015248	MS.gene010656	PPI
MS.gene010656	MS.gene53904	PPI
MS.gene010656	MS.gene24070	PPI
MS.gene010656	MS.gene83772	PPI
MS.gene010656	MS.gene73261	PPI
MS.gene010656	MS.gene059736	PPI
MS.gene010656	MS.gene85538	PPI
MS.gene010656	MS.gene025675	PPI
MS.gene013193	MS.gene010656	PPI
MS.gene08106	MS.gene010656	PPI
MS.gene010656	MS.gene055546	PPI
MS.gene010656	MS.gene71637	PPI
MS.gene010656	MS.gene55209	PPI
MS.gene010656	MS.gene96174	PPI
MS.gene010656	MS.gene074257	PPI
MS.gene010656	MS.gene073573	PPI
MS.gene067340	MS.gene010656	PPI
MS.gene010656	MS.gene28718	PPI
MS.gene010656	MS.gene63743	PPI
MS.gene010656	MS.gene068629	PPI
MS.gene010656	MS.gene009781	PPI
MS.gene062067	MS.gene010656	PPI
MS.gene57044	MS.gene010656	PPI
MS.gene010656	MS.gene21066	PPI
MS.gene010656	MS.gene009213	PPI
MS.gene010656	MS.gene013890	PPI
MS.gene010656	MS.gene98127	PPI
MS.gene010656	MS.gene073582	PPI
MS.gene010656	MS.gene003264	PPI
MS.gene010656	MS.gene054652	PPI
MS.gene038676	MS.gene010656	PPI
MS.gene010656	MS.gene86849	PPI
MS.gene010656	MS.gene048706	PPI
MS.gene042651	MS.gene010656	PPI
MS.gene010656	MS.gene45083	PPI
MS.gene010656	MS.gene049561	PPI
MS.gene010656	MS.gene008658	PPI
MS.gene010656	MS.gene47792	PPI
MS.gene97410	MS.gene010656	PPI
MS.gene010656	MS.gene80362	PPI
MS.gene010656	MS.gene49057	PPI
MS.gene010656	MS.gene89815	PPI
MS.gene010656	MS.gene43393	PPI
MS.gene010656	MS.gene048088	PPI
MS.gene010656	MS.gene86868	PPI
MS.gene010656	MS.gene058889	PPI
MS.gene010656	MS.gene034643	PPI
MS.gene04749	MS.gene010656	PPI
MS.gene010656	MS.gene60059	PPI
MS.gene010656	MS.gene012889	PPI
MS.gene010656	MS.gene34463	PPI
MS.gene010656	MS.gene038757	PPI
MS.gene010656	MS.gene000506	PPI
MS.gene42903	MS.gene010656	PPI
MS.gene010656	MS.gene51804	PPI
MS.gene048747	MS.gene010656	PPI
MS.gene010656	MS.gene013644	PPI
MS.gene010656	MS.gene54051	PPI
MS.gene22200	MS.gene010656	PPI
MS.gene010656	MS.gene010890	PPI
MS.gene010656	MS.gene061579	PPI
MS.gene031967	MS.gene010656	PPI
MS.gene34812	MS.gene010656	PPI
MS.gene063214	MS.gene010656	PPI
MS.gene010656	MS.gene04970	PPI
MS.gene32415	MS.gene010656	PPI
MS.gene035626	MS.gene010656	PPI
MS.gene010656	MS.gene26038	PPI
MS.gene010656	MS.gene060788	PPI
MS.gene010656	MS.gene43924	PPI
MS.gene010656	MS.gene41643	PPI
MS.gene010656	MS.gene008861	PPI
MS.gene010656	MS.gene027994	PPI
MS.gene010656	MS.gene022929	PPI
MS.gene010656	MS.gene85295	PPI
MS.gene69495	MS.gene010656	PPI
MS.gene010656	MS.gene26433	PPI
MS.gene010656	MS.gene007022	PPI
MS.gene010656	MS.gene064966	PPI
MS.gene038623	MS.gene010656	PPI
MS.gene010656	MS.gene69584	PPI
MS.gene05357	MS.gene010656	PPI
MS.gene010656	MS.gene52349	PPI
MS.gene032311	MS.gene010656	PPI
MS.gene010656	MS.gene021647	PPI
MS.gene030090	MS.gene010656	PPI
MS.gene010656	MS.gene047954	PPI
MS.gene010656	MS.gene65471	PPI
MS.gene018196	MS.gene010656	PPI
MS.gene010656	MS.gene071784	PPI
MS.gene010656	MS.gene048342	PPI
MS.gene010656	MS.gene069624	PPI
MS.gene010656	MS.gene060386	PPI
MS.gene57019	MS.gene010656	PPI
MS.gene010656	MS.gene83979	PPI
MS.gene021968	MS.gene010656	PPI
MS.gene010656	MS.gene63762	PPI
MS.gene010656	MS.gene016719	PPI
MS.gene010656	MS.gene048072	PPI
MS.gene010656	MS.gene028714	PPI
MS.gene010656	MS.gene88393	PPI
MS.gene010656	MS.gene042027	PPI
MS.gene09589	MS.gene010656	PPI
MS.gene010656	MS.gene69586	PPI
MS.gene010656	MS.gene053373	PPI
MS.gene43393	MS.gene010656	PPI
MS.gene010656	MS.gene35175	PPI
MS.gene010656	MS.gene63150	PPI
MS.gene008275	MS.gene010656	PPI
MS.gene010656	MS.gene03936	PPI
MS.gene010656	MS.gene44083	PPI
MS.gene010656	MS.gene000629	PPI
MS.gene010656	MS.gene072852	PPI
MS.gene010656	MS.gene65474	PPI
MS.gene010656	MS.gene08708	PPI
MS.gene048072	MS.gene010656	PPI
MS.gene010656	MS.gene04134	PPI
MS.gene010656	MS.gene06164	PPI
MS.gene010656	MS.gene50392	PPI
MS.gene010656	MS.gene86275	PPI
MS.gene010656	MS.gene22730	PPI
MS.gene010656	MS.gene032299	PPI
MS.gene010656	MS.gene017586	PPI
MS.gene05436	MS.gene010656	PPI
MS.gene010656	MS.gene038098	PPI
MS.gene010656	MS.gene037634	PPI
MS.gene010656	MS.gene057828	PPI
MS.gene010656	MS.gene071320	PPI
MS.gene010656	MS.gene08606	PPI
MS.gene010656	MS.gene012019	PPI
MS.gene010656	MS.gene000640	PPI
MS.gene010656	MS.gene061850	PPI
MS.gene010656	MS.gene98756	PPI
MS.gene055683	MS.gene010656	PPI
MS.gene010656	MS.gene39956	PPI
MS.gene010656	MS.gene028716	PPI
MS.gene010656	MS.gene74639	PPI
MS.gene010656	MS.gene071133	PPI
MS.gene010656	MS.gene64130	PPI
MS.gene010656	MS.gene60266	PPI
MS.gene010656	MS.gene98467	PPI
MS.gene010656	MS.gene62249	PPI
MS.gene010656	MS.gene024522	PPI
MS.gene96109	MS.gene010656	PPI
MS.gene010656	MS.gene22111	PPI
MS.gene010656	MS.gene046089	PPI
MS.gene010656	MS.gene049610	PPI
MS.gene010656	MS.gene010290	PPI
MS.gene010656	MS.gene37955	PPI
MS.gene010656	MS.gene92517	PPI
MS.gene010656	MS.gene34197	PPI
MS.gene010656	MS.gene36069	PPI
MS.gene010656	MS.gene045875	PPI
MS.gene010656	MS.gene84790	PPI
MS.gene94654	MS.gene010656	PPI
MS.gene010656	MS.gene71675	PPI
MS.gene010656	MS.gene055683	PPI
MS.gene010656	MS.gene044729	PPI
MS.gene010656	MS.gene05319	PPI
MS.gene010656	MS.gene60390	PPI
MS.gene010656	MS.gene039692	PPI
MS.gene010656	MS.gene032311	PPI
MS.gene010656	MS.gene55682	PPI
MS.gene010656	MS.gene002590	PPI
MS.gene010656	MS.gene41979	PPI
MS.gene83772	MS.gene010656	PPI
MS.gene010656	MS.gene008927	PPI
MS.gene016102	MS.gene010656	PPI
MS.gene46941	MS.gene010656	PPI
MS.gene96541	MS.gene010656	PPI
MS.gene010656	MS.gene98616	PPI
MS.gene010656	MS.gene23521	PPI
MS.gene010656	MS.gene77990	PPI
MS.gene63762	MS.gene010656	PPI
MS.gene010656	MS.gene65546	PPI
MS.gene010656	MS.gene062511	PPI
MS.gene30208	MS.gene010656	PPI
MS.gene010656	MS.gene059269	PPI
MS.gene025873	MS.gene010656	PPI
MS.gene012019	MS.gene010656	PPI
MS.gene010656	MS.gene057934	PPI
MS.gene010656	MS.gene045614	PPI
MS.gene010656	MS.gene024841	PPI
MS.gene05358	MS.gene010656	PPI
MS.gene010656	MS.gene76420	PPI
MS.gene95561	MS.gene010656	PPI
MS.gene010656	MS.gene46353	PPI
MS.gene010656	MS.gene40828	PPI
MS.gene010656	MS.gene87529	PPI
MS.gene065627	MS.gene010656	PPI
MS.gene010656	MS.gene51799	PPI
MS.gene010656	MS.gene98471	PPI
MS.gene010656	MS.gene062067	PPI
MS.gene010656	MS.gene61651	PPI
MS.gene000629	MS.gene010656	PPI
MS.gene010656	MS.gene013676	PPI
MS.gene010656	MS.gene29406	PPI
MS.gene05319	MS.gene010656	PPI
MS.gene038098	MS.gene010656	PPI
MS.gene56991	MS.gene010656	PPI
MS.gene010656	MS.gene09023	PPI
MS.gene010656	MS.gene054508	PPI
MS.gene010656	MS.gene025987	PPI
MS.gene60059	MS.gene010656	PPI
MS.gene010656	MS.gene95960	PPI
MS.gene010656	MS.gene043140	PPI
MS.gene010656	MS.gene041264	PPI
MS.gene28545	MS.gene010656	PPI
MS.gene010656	MS.gene61452	PPI
MS.gene010656	MS.gene03149	PPI
MS.gene010656	MS.gene29321	PPI
MS.gene064966	MS.gene010656	PPI
MS.gene010656	MS.gene018745	PPI
MS.gene010656	MS.gene025401	PPI
MS.gene010656	MS.gene57044	PPI
MS.gene010656	MS.gene015093	PPI
MS.gene010656	MS.gene24278	PPI
MS.gene010656	MS.gene000379	PPI
MS.gene010656	MS.gene34618	PPI
MS.gene010656	MS.gene59460	PPI
MS.gene010656	MS.gene66650	PPI
MS.gene010656	MS.gene009121	PPI
MS.gene036423	MS.gene010656	PPI
MS.gene010656	MS.gene08713	PPI
MS.gene010656	MS.gene70932	PPI
MS.gene010656	MS.gene09517	PPI
MS.gene010656	MS.gene87845	PPI
MS.gene010656	MS.gene024405	PPI
MS.gene010656	MS.gene85308	PPI
MS.gene72520	MS.gene010656	PPI
MS.gene010656	MS.gene58419	PPI
MS.gene66651	MS.gene010656	PPI
MS.gene010656	MS.gene26844	PPI
MS.gene010656	MS.gene64178	PPI
MS.gene051449	MS.gene010656	PPI
MS.gene010656	MS.gene04749	PPI
MS.gene010656	MS.gene053747	PPI
MS.gene010656	MS.gene021555	PPI
MS.gene010656	MS.gene44144	PPI
MS.gene010656	MS.gene05576	PPI
MS.gene010656	MS.gene060718	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010656.t1	MTR_6g033215	84.021	194	31	0	2	195	102	295	8.50e-115	350
MS.gene010656.t1	MTR_8g470880	83.750	160	26	0	614	773	365	524	1.83e-89	292
MS.gene010656.t1	MTR_6g009010	54.634	205	77	1	586	790	65	253	3.01e-74	243
MS.gene010656.t1	MTR_6g053790	53.012	249	69	5	131	368	35	246	2.04e-67	226
MS.gene010656.t1	MTR_0042s0130	51.961	204	92	2	586	786	81	281	2.55e-67	227
MS.gene010656.t1	MTR_2g018005	58.788	165	68	0	586	750	64	228	6.56e-65	216
MS.gene010656.t1	MTR_1g100687	56.497	177	75	1	612	786	4	180	9.42e-65	216
MS.gene010656.t1	MTR_4g044433	80.870	115	22	0	584	698	33	147	3.89e-63	209
MS.gene010656.t1	MTR_6g034215	48.058	206	89	1	586	791	64	251	7.65e-61	209
MS.gene010656.t1	MTR_0114s0080	83.333	114	19	0	642	755	21	134	1.22e-59	199
MS.gene010656.t1	MTR_2g055450	45.833	216	110	1	553	768	72	280	4.74e-57	201
MS.gene010656.t1	MTR_4g020630	52.432	185	80	1	602	786	2	178	1.12e-56	203
MS.gene010656.t1	MTR_2g078880	53.049	164	62	1	586	749	41	189	2.13e-55	190
MS.gene010656.t1	MTR_6g034715	54.110	146	67	0	617	762	1	146	5.30e-52	179
MS.gene010656.t1	MTR_6g046587	45.556	180	97	1	596	775	102	280	9.34e-48	171
MS.gene010656.t1	MTR_1g051330	42.703	185	105	1	602	786	213	396	5.04e-46	176
MS.gene010656.t1	MTR_3g044860	61.017	118	46	0	655	772	3	120	5.41e-45	164
MS.gene010656.t1	MTR_8g463110	52.419	124	59	0	663	786	119	242	1.86e-37	150
MS.gene010656.t1	MTR_0093s0080	45.385	130	49	2	621	750	16	123	3.35e-31	119
MS.gene010656.t1	MTR_0010s0370	31.609	174	117	1	1	172	4	177	9.00e-28	111
MS.gene010656.t1	MTR_0010s0350	31.609	174	117	1	1	172	4	177	9.00e-28	111
MS.gene010656.t1	MTR_8g468010	71.429	63	18	0	110	172	1	63	3.30e-27	108
MS.gene010656.t1	MTR_4g033730	51.456	103	50	0	615	717	36	138	1.76e-26	105
MS.gene010656.t1	MTR_8g446890	37.500	152	66	3	638	789	3	125	4.10e-25	102
MS.gene010656.t1	MTR_1g032270	63.235	68	25	0	93	160	1	68	8.63e-25	101
MS.gene010656.t1	MTR_4g075740	42.177	147	71	5	2	146	22	156	3.67e-24	100
MS.gene010656.t1	MTR_6g053230	28.261	184	93	2	1	181	22	169	2.21e-23	105
MS.gene010656.t1	MTR_2g064335	41.732	127	64	1	624	750	15	131	4.07e-22	98.6
MS.gene010656.t1	MTR_8g019640	36.879	141	50	2	646	786	7	108	6.95e-22	99.4
MS.gene010656.t1	MTR_1g062290	69.492	59	18	0	730	788	1	59	1.23e-21	93.2
MS.gene010656.t1	MTR_3g053920	35.577	104	67	0	22	125	29	132	1.63e-20	92.8
MS.gene010656.t1	MTR_0112s0060	56.944	72	31	0	679	750	36	107	8.16e-20	86.3
MS.gene010656.t1	MTR_0123s0050	66.000	50	17	0	697	746	1	50	2.66e-18	80.5
MS.gene010656.t1	MTR_3g024240	31.667	120	80	1	77	194	55	174	4.18e-18	83.6
MS.gene010656.t1	MTR_8g069750	44.706	85	47	0	587	671	37	121	8.60e-18	84.7
MS.gene010656.t1	MTR_4g020545	27.500	200	93	3	57	256	80	227	1.02e-17	85.1
MS.gene010656.t1	MTR_4g103530	39.326	89	54	0	52	140	5	93	1.34e-17	82.0
MS.gene010656.t1	MTR_2g061500	32.673	101	68	0	22	122	29	129	1.71e-17	80.1
MS.gene010656.t1	MTR_2g084625	43.333	90	49	1	622	709	12	101	6.82e-17	83.2
MS.gene010656.t1	MTR_8g442690	30.114	176	114	3	625	798	50	218	5.24e-16	82.0
MS.gene010656.t1	MTR_3g041640	76.190	42	10	0	110	151	1	42	6.15e-16	75.9
MS.gene010656.t1	MTR_1g054775	29.412	204	141	3	588	789	617	819	6.84e-16	83.2
MS.gene010656.t1	MTR_4g122570	34.921	126	80	2	681	806	328	451	4.63e-13	73.2
MS.gene010656.t1	MTR_7g011710	32.099	81	55	0	52	132	5	85	3.71e-11	62.8
MS.gene010656.t1	MTR_1g036710	40.323	62	37	0	586	647	233	294	8.98e-11	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010656.t1	AT2G17920	24.832	149	110	1	62	210	72	218	7.13e-13	70.9
MS.gene010656.t1	AT2G01050	21.858	183	137	2	28	210	80	256	4.26e-12	70.1
MS.gene010656.t1	AT5G18636	22.222	198	152	1	26	223	36	231	7.41e-12	68.6
MS.gene010656.t1	AT3G31430	25.543	184	133	1	27	210	132	311	2.13e-11	66.6

Find 227 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATGGAAGACAACAAGTTTA+TGG	0.178877	4.4:+82645397	MS.gene010656:CDS
TTTAACTTTCTTTAAGTATA+TGG	0.222104	4.4:+82646108	MS.gene010656:CDS
CCATGAATCCGAACGGTAAT+TGG	0.250195	4.4:-82645571	None:intergenic
GCATTGAAAGAGACATTTAT+TGG	0.252994	4.4:-82646650	None:intergenic
AGGCGAGGCTCGGTCCTATT+TGG	0.260583	4.4:+82645345	MS.gene010656:CDS
CAGCTCTGGCTCGGCTTGGC+TGG	0.283842	4.4:-82646814	None:intergenic
ATTTCAAACCACAAGGTATA+CGG	0.288491	4.4:+82646049	MS.gene010656:CDS
CCAATTACCGTTCGGATTCA+TGG	0.302195	4.4:+82645571	MS.gene010656:CDS
CTATGAATACTACGGAGATT+TGG	0.305516	4.4:+82645537	MS.gene010656:CDS
GTGGAGGTATTGCTTTGTTT+TGG	0.317172	4.4:+82647317	MS.gene010656:CDS
TTTACTTGGTTTAAAAGTTT+GGG	0.323694	4.4:+82647661	MS.gene010656:CDS
CTGGCTTGAGTTGTCCTTTG+TGG	0.323699	4.4:-82646795	None:intergenic
TGATACTTAGGTTCCCAAAT+AGG	0.324421	4.4:-82645359	None:intergenic
TGGTCTGACTTTGTAAGGTT+TGG	0.326927	4.4:-82646250	None:intergenic
TTGCTTGATCTTTGCCTTGT+TGG	0.327557	4.4:+82645281	MS.gene010656:CDS
GTCTCTTTCAATGCTAATAA+TGG	0.330090	4.4:+82646659	MS.gene010656:CDS
TAGGAAGAAGAAGAGATTCT+TGG	0.345146	4.4:+82647455	MS.gene010656:CDS
TTGAGGCCCACCAGCTCTAC+TGG	0.349397	4.4:-82646948	None:intergenic
CATCAAGCAGTGAGAAGAAA+TGG	0.355322	4.4:+82645864	MS.gene010656:CDS
CTGGCAGCTCTGGCTCGGCT+TGG	0.362237	4.4:-82646818	None:intergenic
GGTTAACAAACCTATTCATT+TGG	0.363677	4.4:+82645313	MS.gene010656:CDS
CACTCCCGACCCAGCTATCT+TGG	0.367565	4.4:-82647903	None:intergenic
GGCGAGGCTCGGTCCTATTT+GGG	0.370002	4.4:+82645346	MS.gene010656:CDS
TGACTTGGTCTGACTTTGTA+AGG	0.370307	4.4:-82646255	None:intergenic
AGACCAAACTGGCTTATTAG+AGG	0.371312	4.4:+82647580	MS.gene010656:CDS
TATGAATACTACGGAGATTT+GGG	0.376905	4.4:+82645538	MS.gene010656:CDS
CGAACTTCGATCAAGGATTC+TGG	0.378262	4.4:-82645447	None:intergenic
TGCGTGGCTTCCCATGCTGC+TGG	0.381360	4.4:-82646865	None:intergenic
TATTTAGAGGCCCAACAAAA+AGG	0.389575	4.4:+82646356	MS.gene010656:intron
TTTGCCTCATATTGTCGAAA+TGG	0.390610	4.4:-82648077	None:intergenic
CTGAGTGAGGTTCGGCCTCT+TGG	0.391003	4.4:-82645252	None:intergenic
AGGAAGCCCACAATGTTCTA+AGG	0.394503	4.4:+82646376	MS.gene010656:CDS
TGACATTCAAAGTCATTCAT+TGG	0.400619	4.4:+82646009	MS.gene010656:CDS
GGCAATTGGTCCTGTTCGAT+GGG	0.403697	4.4:+82646510	MS.gene010656:CDS
TGTTCTTGACCTCTTCTTGA+GGG	0.410060	4.4:-82646210	None:intergenic
GTCACATTGGTAAAATGATT+AGG	0.416016	4.4:+82645621	MS.gene010656:CDS
AATCCTCTAATAAGCCAGTT+TGG	0.418863	4.4:-82647583	None:intergenic
ATAAAAGTGCCAGGCTGCAC+TGG	0.421529	4.4:-82646908	None:intergenic
GCTGACGATTGCTGCAATAA+TGG	0.422624	4.4:-82646575	None:intergenic
CAAATCTCCGTAGTATTCAT+AGG	0.423177	4.4:-82645536	None:intergenic
CCACCTGTAGTGTCTTCCAT+AGG	0.425032	4.4:-82646184	None:intergenic
ATCAACGTCATTTGATACTT+AGG	0.427652	4.4:-82645371	None:intergenic
ACAAAAGAAGCATGGGCAAT+TGG	0.428802	4.4:+82646496	MS.gene010656:CDS
AATAAGACCGACTTCTTGAA+TGG	0.431242	4.4:-82647614	None:intergenic
ACTGATGATTGGTCATCATT+AGG	0.432376	4.4:-82646725	None:intergenic
ATCAAGCAGTGAGAAGAAAT+GGG	0.432846	4.4:+82645865	MS.gene010656:CDS
ACTGATGGGCCCCATCGAAC+AGG	0.436614	4.4:-82646520	None:intergenic
ATGACTTTGAATGTCATTGA+TGG	0.444935	4.4:-82646002	None:intergenic
GGGCAATTGGTCCTGTTCGA+TGG	0.448461	4.4:+82646509	MS.gene010656:CDS
GTGGGCCTCAAGAGGAAGCT+GGG	0.451005	4.4:+82646960	MS.gene010656:CDS
AAGAAGTCAATCGTAGGGCT+TGG	0.452082	4.4:+82647401	MS.gene010656:CDS
TTCCTTGTTGCCACCACCAA+CGG	0.457126	4.4:-82645915	None:intergenic
GTATTCATAGGCATCGTCAT+AGG	0.460360	4.4:-82645524	None:intergenic
GGTGGGCCTCAAGAGGAAGC+TGG	0.460577	4.4:+82646959	MS.gene010656:CDS
CCGCACCGCTCACGGAATCC+AGG	0.462891	4.4:+82648047	MS.gene010656:CDS
ATGAGGTAGTCCAAGATAGC+TGG	0.465731	4.4:+82647893	MS.gene010656:CDS
TTAATGATTGTTTGACATGT+TGG	0.466186	4.4:-82646550	None:intergenic
ACCTATTCATTTGGCTACGT+TGG	0.471123	4.4:+82645322	MS.gene010656:CDS
GCACGTGGGGCCAGTAGAGC+TGG	0.474919	4.4:+82646938	MS.gene010656:CDS
ACTGGGTTGCGAGCTATGAC+TGG	0.475349	4.4:-82646890	None:intergenic
TATTCATTTGGCTACGTTGG+AGG	0.475511	4.4:+82645325	MS.gene010656:CDS
TGTCGAAATGGACCTCATCC+TGG	0.477300	4.4:-82648065	None:intergenic
GTTCGGATTCATGGATGAAA+AGG	0.479331	4.4:+82645580	MS.gene010656:CDS
CACCAACCTTAGAACATTGT+GGG	0.479354	4.4:-82646382	None:intergenic
GAGGTTCTGTCGTGCAAGAT+AGG	0.485504	4.4:-82647799	None:intergenic
TTAGGCCCAGCTTCCTCTTG+AGG	0.485984	4.4:-82646965	None:intergenic
TGTCAAAGATCAGTCGGAAC+GGG	0.486348	4.4:+82646133	MS.gene010656:CDS
CATGTTGGTCTGCAGACTGA+TGG	0.487812	4.4:-82646535	None:intergenic
AAATCTACCTTCGTGTCTGA+TGG	0.488411	4.4:+82647507	MS.gene010656:CDS
CGAACGGTAATTGGTGTACC+TGG	0.489553	4.4:-82645562	None:intergenic
GAGCCTTGGTCACTAACTCT+TGG	0.490541	4.4:+82647716	MS.gene010656:CDS
TTCGATCAAGGATTCTGGTC+AGG	0.491440	4.4:-82645442	None:intergenic
TCATTGGCATTCAATGATAA+CGG	0.493005	4.4:+82646025	MS.gene010656:CDS
TTGTTCTTGACCTCTTCTTG+AGG	0.496829	4.4:-82646211	None:intergenic
ATTCTAGAGAAAGTTGCTGT+TGG	0.499846	4.4:+82645494	MS.gene010656:CDS
TGGCTTCCCATGCTGCTGGA+TGG	0.499984	4.4:-82646861	None:intergenic
GAGAACAACGCCGTTGGTGG+TGG	0.500337	4.4:+82645905	MS.gene010656:CDS
GCTCGCAACCCAGTGCAGCC+TGG	0.504227	4.4:+82646899	MS.gene010656:CDS
TCGAGCTAAAATTGCGGTGG+AGG	0.504749	4.4:+82645691	MS.gene010656:CDS
GAACGTGACGTTGGAAAGGA+GGG	0.508603	4.4:+82647016	MS.gene010656:CDS
AGGAAGCTGGGCCTAAGAAA+AGG	0.511062	4.4:+82646972	MS.gene010656:CDS
GGTGGAGGTACCACTACAAC+AGG	0.511575	4.4:+82645706	MS.gene010656:CDS
TACTACGGAGATTTGGGTCC+AGG	0.511672	4.4:+82645544	MS.gene010656:CDS
TCCAACGTAGCCAAATGAAT+AGG	0.512074	4.4:-82645323	None:intergenic
GCTACGTTGGAGGCGAGGCT+CGG	0.512129	4.4:+82645335	MS.gene010656:CDS
AGGTCCATTTCGACAATATG+AGG	0.512273	4.4:+82648073	MS.gene010656:CDS
TTGCGGCCATCCAGCAGCAT+GGG	0.512349	4.4:+82646855	MS.gene010656:CDS
TCAAATGACGTTGATTCTCA+TGG	0.512613	4.4:+82645379	MS.gene010656:CDS
GGAGGGCCACTAGACCAAAC+TGG	0.517579	4.4:+82647569	MS.gene010656:CDS
CAGGTACACCAATTACCGTT+CGG	0.521592	4.4:+82645563	MS.gene010656:CDS
TATGGTTCAGCTGTATTCAA+AGG	0.523312	4.4:+82645415	MS.gene010656:CDS
CTTATTGATGTTCACATGGA+GGG	0.524060	4.4:+82647631	MS.gene010656:CDS
GTGGACGGAGATCGACCCTA+TGG	0.524859	4.4:+82646168	MS.gene010656:CDS
CGTAGGGCTTGGATATTCAC+AGG	0.526969	4.4:+82647412	MS.gene010656:CDS
GCAACCACGAACTTCGATCA+AGG	0.527669	4.4:-82645454	None:intergenic
AATTCAGCCTGAGTGAGGTT+CGG	0.531756	4.4:-82645260	None:intergenic
GTTCTTGACCTCTTCTTGAG+GGG	0.533226	4.4:-82646209	None:intergenic
CGGATTCATGGATGAAAAGG+TGG	0.534614	4.4:+82645583	MS.gene010656:CDS
ACAACTACGGGCCATGGCGG+AGG	0.537762	4.4:+82645657	MS.gene010656:CDS
GCTGAGAACAACGCCGTTGG+TGG	0.538326	4.4:+82645902	MS.gene010656:CDS
AGAAAAGTTGGATCGAGCCT+TGG	0.539062	4.4:+82647702	MS.gene010656:CDS
CCTTCAAGTTTGAAAATGCA+TGG	0.539088	4.4:+82647851	MS.gene010656:CDS
CTGGTCATTGAGAGGGAGGA+AGG	0.539887	4.4:+82645731	MS.gene010656:CDS
ATGGAGAGACTTAACATCAA+TGG	0.540353	4.4:+82645212	MS.gene010656:CDS
CTCATCCTGGATTCCGTGAG+CGG	0.544137	4.4:-82648052	None:intergenic
TTTCAAACCACAAGGTATAC+GGG	0.544235	4.4:+82646050	MS.gene010656:CDS
ATAGGAAATTGATCTGATGA+TGG	0.544828	4.4:-82647781	None:intergenic
TAGAGCTGGTGGGCCTCAAG+AGG	0.544829	4.4:+82646952	MS.gene010656:CDS
CGCCGTTGGTGGTGGCAACA+AGG	0.545454	4.4:+82645913	MS.gene010656:CDS
GAATCCTTGATCGAAGTTCG+TGG	0.546146	4.4:+82645450	MS.gene010656:CDS
TAGTCCAAGATAGCTGGGTC+GGG	0.546529	4.4:+82647899	MS.gene010656:CDS
GCACTAGCCATAAAAGTGCC+AGG	0.548834	4.4:-82646917	None:intergenic
TCTGGCAGTCGTAAGGAAGA+AGG	0.549283	4.4:+82645974	MS.gene010656:CDS
CTTAACATCAATGGTGAACA+TGG	0.549393	4.4:+82645221	MS.gene010656:CDS
AATAAGCCAGTTTGGTCTAG+TGG	0.550288	4.4:-82647575	None:intergenic
AGGAAAACAACTACGGGCCA+TGG	0.550400	4.4:+82645651	MS.gene010656:CDS
AGTAGAAGCCGCACCGCTCA+CGG	0.553490	4.4:+82648039	MS.gene010656:CDS
GAAAGCAGCTAGCCGACAGT+AGG	0.554305	4.4:+82646444	MS.gene010656:CDS
CGCTCACGGAATCCAGGATG+AGG	0.554376	4.4:+82648053	MS.gene010656:CDS
TAAAAGTGCCAGGCTGCACT+GGG	0.555045	4.4:-82646907	None:intergenic
GACGATGCCTATGAATACTA+CGG	0.555870	4.4:+82645529	MS.gene010656:CDS
CGTGGGGCCAGTAGAGCTGG+TGG	0.556002	4.4:+82646941	MS.gene010656:CDS
CGAGCTATGACTGGCTTGCG+TGG	0.556588	4.4:-82646881	None:intergenic
TTCGATGAGGAAAACAACTA+CGG	0.560349	4.4:+82645644	MS.gene010656:CDS
CCACCAACCTTAGAACATTG+TGG	0.560887	4.4:-82646383	None:intergenic
CTCGAAATCATAGAGTAGAA+AGG	0.563057	4.4:+82647827	MS.gene010656:CDS
GCAAGTGGATCGTCGGTGGT+CGG	0.564747	4.4:+82645942	MS.gene010656:CDS
GTAGTCCAAGATAGCTGGGT+CGG	0.564971	4.4:+82647898	MS.gene010656:CDS
AATCTACCTTCGTGTCTGAT+GGG	0.565014	4.4:+82647508	MS.gene010656:CDS
CGGTCTTATTGATGTTCACA+TGG	0.565244	4.4:+82647627	MS.gene010656:CDS
GTTGCGGCCATCCAGCAGCA+TGG	0.566310	4.4:+82646854	MS.gene010656:CDS
ATGTTGGTCTGCAGACTGAT+GGG	0.566556	4.4:-82646534	None:intergenic
ATTTGGCTACGTTGGAGGCG+AGG	0.566565	4.4:+82645330	MS.gene010656:CDS
TTGCCTTGTTGGTCGAGTGA+TGG	0.568832	4.4:+82645292	MS.gene010656:CDS
AGCCCACAATGTTCTAAGGT+TGG	0.572090	4.4:+82646380	MS.gene010656:CDS
CAAGAGGCCGAACCTCACTC+AGG	0.573873	4.4:+82645253	MS.gene010656:CDS
TCGGAACGGGCGTAGTGAGA+TGG	0.573888	4.4:+82646146	MS.gene010656:CDS
CGAGAAGCCATTCAAGAAGT+CGG	0.574646	4.4:+82647607	MS.gene010656:CDS
TTATTGATGTTCACATGGAG+GGG	0.575655	4.4:+82647632	MS.gene010656:CDS
CGTCAGCACAAAAGAAGCAT+GGG	0.576256	4.4:+82646489	MS.gene010656:CDS
GTTAGAAATAGTAGAGGTGG+AGG	0.576512	4.4:+82647301	MS.gene010656:intron
CACCTGTAGTGTCTTCCATA+GGG	0.577741	4.4:-82646183	None:intergenic
GACCCTATGGAAGACACTAC+AGG	0.577898	4.4:+82646181	MS.gene010656:CDS
GCGTGCAGTAGAAGAAAAGT+TGG	0.578056	4.4:+82647690	MS.gene010656:CDS
CCTGTTAGAAATAGTAGAGG+TGG	0.581756	4.4:+82647298	MS.gene010656:intron
ATGGAATGTCAAAGATCAGT+CGG	0.583669	4.4:+82646127	MS.gene010656:CDS
TGAGGTAGTCCAAGATAGCT+GGG	0.583959	4.4:+82647894	MS.gene010656:CDS
ATGACCAGCTCCTGTTGTAG+TGG	0.585093	4.4:-82645716	None:intergenic
ACGTCAGCACAAAAGAAGCA+TGG	0.587538	4.4:+82646488	MS.gene010656:CDS
AATTGAAGAAGTCAATCGTA+GGG	0.588210	4.4:+82647396	MS.gene010656:CDS
CGTGGCACAAGTGGTGAGGG+TGG	0.589203	4.4:+82646422	MS.gene010656:CDS
GCGCGTGGCACAAGTGGTGA+GGG	0.590297	4.4:+82646419	MS.gene010656:CDS
TGCATGGAGAATCGAGGAGG+GGG	0.590522	4.4:+82647867	MS.gene010656:CDS
GAAAAGGTGGGTGCCCTCGT+TGG	0.590864	4.4:+82645596	MS.gene010656:CDS
GACGTCATGCTGTCCACAGG+TGG	0.590898	4.4:-82646470	None:intergenic
GTATACGGGCGCATTAAAGT+AGG	0.591679	4.4:+82646064	MS.gene010656:CDS
CCACAATGTTCTAAGGTTGG+TGG	0.592077	4.4:+82646383	MS.gene010656:CDS
GCCCTCGTTGGTAGTCACAT+TGG	0.592684	4.4:+82645608	MS.gene010656:CDS
AGAGAAAGTTGCTGTTGGAG+AGG	0.595289	4.4:+82645499	MS.gene010656:CDS
GGTACCACTACAACAGGAGC+TGG	0.596643	4.4:+82645712	MS.gene010656:CDS
TGAAGAACGTGACGTTGGAA+AGG	0.596864	4.4:+82647012	MS.gene010656:CDS
TCGATGAGGAAAACAACTAC+GGG	0.597705	4.4:+82645645	MS.gene010656:CDS
CAGAAAAGACAAAGGAGTTG+CGG	0.598470	4.4:+82646838	MS.gene010656:CDS
AGCGCGTGGCACAAGTGGTG+AGG	0.598523	4.4:+82646418	MS.gene010656:CDS
AGAACGTGACGTTGGAAAGG+AGG	0.598661	4.4:+82647015	MS.gene010656:CDS
GGAGCTGGTCATTGAGAGGG+AGG	0.599342	4.4:+82645727	MS.gene010656:CDS
TAGGTATTAATGCAGAGACA+AGG	0.601650	4.4:+82646083	MS.gene010656:CDS
AGGTGGGGCCCCTCAAGAAG+AGG	0.601868	4.4:+82646201	MS.gene010656:CDS
GCTGACGTCATGCTGTCCAC+AGG	0.602576	4.4:-82646473	None:intergenic
TTTGCTGAAGAACGTGACGT+TGG	0.602582	4.4:+82647007	MS.gene010656:CDS
TAGTCAGTAGCTTTGAACAT+GGG	0.603734	4.4:-82646755	None:intergenic
AAATTGAAGAAGTCAATCGT+AGG	0.605963	4.4:+82647395	MS.gene010656:CDS
AACAGGAGCTGGTCATTGAG+AGG	0.606374	4.4:+82645723	MS.gene010656:CDS
GAAGCAAGCAAGTGGATCGT+CGG	0.607313	4.4:+82645935	MS.gene010656:CDS
AAAGCTGAGAACAACGCCGT+TGG	0.608586	4.4:+82645899	MS.gene010656:CDS
TCTTATTGATGTTCACATGG+AGG	0.610077	4.4:+82647630	MS.gene010656:CDS
GCACCTGTTAGAAATAGTAG+AGG	0.620923	4.4:+82647295	MS.gene010656:intron
CGACAGTAGGGTCCCACCTG+TGG	0.621010	4.4:+82646457	MS.gene010656:CDS
GGGCGTAGTGAGATGGTGGA+CGG	0.621311	4.4:+82646153	MS.gene010656:CDS
TAATGCGCCCGTATACCTTG+TGG	0.622166	4.4:-82646057	None:intergenic
GCAACAAGGAAGCAAGCAAG+TGG	0.625107	4.4:+82645927	MS.gene010656:CDS
ATGTCAAAGATCAGTCGGAA+CGG	0.627385	4.4:+82646132	MS.gene010656:CDS
AATGCATGGAGAATCGAGGA+GGG	0.627574	4.4:+82647865	MS.gene010656:CDS
AAAGCAGCTAGCCGACAGTA+GGG	0.627613	4.4:+82646445	MS.gene010656:CDS
AGTCAGTAGCTTTGAACATG+GGG	0.627860	4.4:-82646754	None:intergenic
ACAGGAGCTGGTCATTGAGA+GGG	0.630702	4.4:+82645724	MS.gene010656:CDS
TAAAATGATTAGGTTCGATG+AGG	0.630764	4.4:+82645631	MS.gene010656:CDS
GAACGGGCGTAGTGAGATGG+TGG	0.630861	4.4:+82646149	MS.gene010656:CDS
GATGTAGCGCGTGGCACAAG+TGG	0.631389	4.4:+82646413	MS.gene010656:CDS
GGATTCATGGATGAAAAGGT+GGG	0.631464	4.4:+82645584	MS.gene010656:CDS
GTAGTCAGTAGCTTTGAACA+TGG	0.632738	4.4:-82646756	None:intergenic
GTGGGGCCAGTAGAGCTGGT+GGG	0.634707	4.4:+82646942	MS.gene010656:CDS
CCTATGGAAGACACTACAGG+TGG	0.636035	4.4:+82646184	MS.gene010656:CDS
AGGTATTAATGCAGAGACAA+GGG	0.638280	4.4:+82646084	MS.gene010656:CDS
AGAGCTGCCAGAAAAGACAA+AGG	0.642200	4.4:+82646830	MS.gene010656:CDS
ACGTCATGCTGTCCACAGGT+GGG	0.642220	4.4:-82646469	None:intergenic
TATGGAAGACACTACAGGTG+GGG	0.644155	4.4:+82646186	MS.gene010656:CDS
CCTGGATTCCGTGAGCGGTG+CGG	0.646666	4.4:-82648047	None:intergenic
GGTTCAGCTGTATTCAAAGG+CGG	0.647402	4.4:+82645418	MS.gene010656:CDS
GCAAGCAAGTGGATCGTCGG+TGG	0.647580	4.4:+82645938	MS.gene010656:CDS
AGTTCGAGCTAAAATTGCGG+TGG	0.648723	4.4:+82645688	MS.gene010656:CDS
TTGTGGAGACAAATTCTGCA+CGG	0.651186	4.4:-82646778	None:intergenic
CATCCAAGAGTTAGTGACCA+AGG	0.651962	4.4:-82647719	None:intergenic
TCTATGATTTCGAGCTACCG+AGG	0.652135	4.4:-82647818	None:intergenic
CAAGCAATTCAGCCTGAGTG+AGG	0.652525	4.4:-82645265	None:intergenic
CACAGGTGGGACCCTACTGT+CGG	0.660763	4.4:-82646456	None:intergenic
CAGTCGTAAGGAAGAAGGAG+CGG	0.662364	4.4:+82645979	MS.gene010656:CDS
ACCAATGTGACTACCAACGA+GGG	0.664109	4.4:-82645609	None:intergenic
TAACCATCACTCGACCAACA+AGG	0.671446	4.4:-82645295	None:intergenic
TATCTTGCACGACAGAACCT+CGG	0.671990	4.4:+82647801	MS.gene010656:CDS
ACTCTCATAAACCTCCGCCA+TGG	0.674024	4.4:-82645668	None:intergenic
AAATCTCCCATCAGACACGA+AGG	0.674795	4.4:-82647514	None:intergenic
TCAAGCAGTGAGAAGAAATG+GGG	0.678558	4.4:+82645866	MS.gene010656:CDS
TGAAAATGCATGGAGAATCG+AGG	0.678741	4.4:+82647861	MS.gene010656:CDS
GCAATTGGTCCTGTTCGATG+GGG	0.681398	4.4:+82646511	MS.gene010656:CDS
AACGTGACGTTGGAAAGGAG+GGG	0.683592	4.4:+82647017	MS.gene010656:CDS
TAACGGAATTTCAAACCACA+AGG	0.684025	4.4:+82646042	MS.gene010656:CDS
GGCAGCAATGATGTAGCGCG+TGG	0.685162	4.4:+82646404	MS.gene010656:CDS
CTATGGAAGACACTACAGGT+GGG	0.689080	4.4:+82646185	MS.gene010656:CDS
AACATGGTGCTGCATCCAAG+AGG	0.689734	4.4:+82645237	MS.gene010656:CDS
TGCAGAATTTGTCTCCACAA+AGG	0.693159	4.4:+82646781	MS.gene010656:CDS
ATGCATGGAGAATCGAGGAG+GGG	0.695934	4.4:+82647866	MS.gene010656:CDS
AAATGCATGGAGAATCGAGG+AGG	0.696144	4.4:+82647864	MS.gene010656:CDS
GAGAGTTCGAGCTAAAATTG+CGG	0.698583	4.4:+82645685	MS.gene010656:CDS
TACCAATGTGACTACCAACG+AGG	0.709599	4.4:-82645610	None:intergenic
AAAACAACTACGGGCCATGG+CGG	0.725253	4.4:+82645654	MS.gene010656:CDS
GTCAGTAGCTTTGAACATGG+GGG	0.742101	4.4:-82646753	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTGAAAAAAAATGAAA+CGG	+	chr4.4:82647070-82647089	MS.gene010656:intron	10.0%
!!!	TTCATTTTTTTTCAAAAATT+GGG	-	chr4.4:82647069-82647088	None:intergenic	10.0%
!!!	TTTCATTTTTTTTCAAAAAT+TGG	-	chr4.4:82647070-82647089	None:intergenic	10.0%
!!!	TTTAACTTTCTTTAAGTATA+TGG	+	chr4.4:82646108-82646127	MS.gene010656:CDS	15.0%
!!	GAGGAATTTCATTATTTATT+GGG	+	chr4.4:82647256-82647275	MS.gene010656:intron	20.0%
!!	TTCTTGAATCTTATTACAAT+GGG	-	chr4.4:82647992-82648011	None:intergenic	20.0%
!!	TTTACTTGGTTTAAAAGTTT+GGG	+	chr4.4:82647661-82647680	MS.gene010656:CDS	20.0%
!!!	AAACTTTTAAACCAAGTAAA+AGG	-	chr4.4:82647661-82647680	None:intergenic	20.0%
!!!	ATTCTTGGAATTTTATTCTT+GGG	+	chr4.4:82647470-82647489	MS.gene010656:CDS	20.0%
!!!	TTTTACTTGGTTTAAAAGTT+TGG	+	chr4.4:82647660-82647679	MS.gene010656:CDS	20.0%
!!!	TTTTATGGATTTCCAAAAAT+AGG	+	chr4.4:82647436-82647455	MS.gene010656:CDS	20.0%
!	CAATAGTTTCACAAAGAAAA+AGG	-	chr4.4:82647219-82647238	None:intergenic	25.0%
!	CGAGGAATTTCATTATTTAT+TGG	+	chr4.4:82647255-82647274	MS.gene010656:intron	25.0%
!	CTTCTTGAATCTTATTACAA+TGG	-	chr4.4:82647993-82648012	None:intergenic	25.0%
!	TTAATGATTGTTTGACATGT+TGG	-	chr4.4:82646553-82646572	None:intergenic	25.0%
!!	AGATTTTCTAATTTGGCATT+AGG	-	chr4.4:82647754-82647773	None:intergenic	25.0%
!!	AGCAACAAGATTTTCTAATT+TGG	-	chr4.4:82647761-82647780	None:intergenic	25.0%
!!	GATTCTTGGAATTTTATTCT+TGG	+	chr4.4:82647469-82647488	MS.gene010656:CDS	25.0%
!!	TTACCGAAAATTGTTTTCAA+AGG	+	chr4.4:82646619-82646638	MS.gene010656:CDS	25.0%
!!!	ATGGAAGTTGATATATTTAG+AGG	+	chr4.4:82646343-82646362	MS.gene010656:intron	25.0%
!!!	CTTCAATTTTAGCTCTAATA+TGG	-	chr4.4:82647384-82647403	None:intergenic	25.0%
!!!	GTTTTCAAGTATGAGAAATT+GGG	+	chr4.4:82645770-82645789	MS.gene010656:CDS	25.0%
!!!	TCTCCTTTGAAAACAATTTT+CGG	-	chr4.4:82646625-82646644	None:intergenic	25.0%
!!!	TGTTTTCAAGTATGAGAAAT+TGG	+	chr4.4:82645769-82645788	MS.gene010656:CDS	25.0%
	AAATTGAAGAAGTCAATCGT+AGG	+	chr4.4:82647395-82647414	MS.gene010656:CDS	30.0%
	AATTGAAGAAGTCAATCGTA+GGG	+	chr4.4:82647396-82647415	MS.gene010656:CDS	30.0%
	ATAGGAAATTGATCTGATGA+TGG	-	chr4.4:82647784-82647803	None:intergenic	30.0%
	ATCAACGTCATTTGATACTT+AGG	-	chr4.4:82645374-82645393	None:intergenic	30.0%
	ATGACTTTGAATGTCATTGA+TGG	-	chr4.4:82646005-82646024	None:intergenic	30.0%
	ATTTCAAACCACAAGGTATA+CGG	+	chr4.4:82646049-82646068	MS.gene010656:CDS	30.0%
	CTAAAATCAAATTCCCTGTT+CGG	+	chr4.4:82647176-82647195	MS.gene010656:intron	30.0%
	GCATTGAAAGAGACATTTAT+TGG	-	chr4.4:82646653-82646672	None:intergenic	30.0%
	GGTTAACAAACCTATTCATT+TGG	+	chr4.4:82645313-82645332	MS.gene010656:CDS	30.0%
	GTCACATTGGTAAAATGATT+AGG	+	chr4.4:82645621-82645640	MS.gene010656:CDS	30.0%
	GTCTCTTTCAATGCTAATAA+TGG	+	chr4.4:82646659-82646678	MS.gene010656:CDS	30.0%
	TAAAATGATTAGGTTCGATG+AGG	+	chr4.4:82645631-82645650	MS.gene010656:CDS	30.0%
	TATGAATACTACGGAGATTT+GGG	+	chr4.4:82645538-82645557	MS.gene010656:CDS	30.0%
	TCACAAAGAAAAAGGATATC+CGG	-	chr4.4:82647211-82647230	None:intergenic	30.0%
	TCATTGGCATTCAATGATAA+CGG	+	chr4.4:82646025-82646044	MS.gene010656:CDS	30.0%
	TGACATTCAAAGTCATTCAT+TGG	+	chr4.4:82646009-82646028	MS.gene010656:CDS	30.0%
	TGTTGAAGCTAATAAAATCG+AGG	+	chr4.4:82647237-82647256	MS.gene010656:intron	30.0%
	TTGAAAAAAAATGAAACGGC+CGG	+	chr4.4:82647074-82647093	MS.gene010656:intron	30.0%
!	TCTCTTCTTCTTCCTATTTT+TGG	-	chr4.4:82647451-82647470	None:intergenic	30.0%
!	TGCTAATAATGGTGTTTTTC+AGG	+	chr4.4:82646670-82646689	MS.gene010656:CDS	30.0%
!	TGGATATTCACAGGATTTTA+TGG	+	chr4.4:82647421-82647440	MS.gene010656:CDS	30.0%
!!!	TGCTTTGTTTTGGAGAAATA+CGG	+	chr4.4:82647327-82647346	MS.gene010656:CDS	30.0%
	AAGAAGAGATTGATCATTGC+AGG	+	chr4.4:82648007-82648026	MS.gene010656:intron	35.0%
	AATCCTCTAATAAGCCAGTT+TGG	-	chr4.4:82647586-82647605	None:intergenic	35.0%
	AGGTATTAATGCAGAGACAA+GGG	+	chr4.4:82646084-82646103	MS.gene010656:CDS	35.0%
	ATCAAGCAGTGAGAAGAAAT+GGG	+	chr4.4:82645865-82645884	MS.gene010656:CDS	35.0%
	ATGGAATGTCAAAGATCAGT+CGG	+	chr4.4:82646127-82646146	MS.gene010656:CDS	35.0%
	ATGGAGAGACTTAACATCAA+TGG	+	chr4.4:82645212-82645231	MS.gene010656:CDS	35.0%
	CAAATCTCCGTAGTATTCAT+AGG	-	chr4.4:82645539-82645558	None:intergenic	35.0%
	CATGGAAGACAACAAGTTTA+TGG	+	chr4.4:82645397-82645416	MS.gene010656:CDS	35.0%
	CTATGAATACTACGGAGATT+TGG	+	chr4.4:82645537-82645556	MS.gene010656:CDS	35.0%
	CTCGAAATCATAGAGTAGAA+AGG	+	chr4.4:82647827-82647846	MS.gene010656:CDS	35.0%
	CTTAACATCAATGGTGAACA+TGG	+	chr4.4:82645221-82645240	MS.gene010656:CDS	35.0%
	CTTATTGATGTTCACATGGA+GGG	+	chr4.4:82647631-82647650	MS.gene010656:CDS	35.0%
	TAACGGAATTTCAAACCACA+AGG	+	chr4.4:82646042-82646061	MS.gene010656:CDS	35.0%
	TAGGAAGAAGAAGAGATTCT+TGG	+	chr4.4:82647455-82647474	MS.gene010656:CDS	35.0%
	TAGGTATTAATGCAGAGACA+AGG	+	chr4.4:82646083-82646102	MS.gene010656:CDS	35.0%
	TATGGTTCAGCTGTATTCAA+AGG	+	chr4.4:82645415-82645434	MS.gene010656:CDS	35.0%
	TATTTAGAGGCCCAACAAAA+AGG	+	chr4.4:82646356-82646375	MS.gene010656:intron	35.0%
	TCCATGCTGATGAGAAAAAA+GGG	+	chr4.4:82647548-82647567	MS.gene010656:CDS	35.0%
	TCTTATTGATGTTCACATGG+AGG	+	chr4.4:82647630-82647649	MS.gene010656:CDS	35.0%
	TGATACTTAGGTTCCCAAAT+AGG	-	chr4.4:82645362-82645381	None:intergenic	35.0%
	TTATTGATGTTCACATGGAG+GGG	+	chr4.4:82647632-82647651	MS.gene010656:CDS	35.0%
	TTCCATGCTACAATACTCAT+TGG	-	chr4.4:82647124-82647143	None:intergenic	35.0%
	TTCGATGAGGAAAACAACTA+CGG	+	chr4.4:82645644-82645663	MS.gene010656:CDS	35.0%
	TTTCAAACCACAAGGTATAC+GGG	+	chr4.4:82646050-82646069	MS.gene010656:CDS	35.0%
!	AATAAGACCGACTTCTTGAA+TGG	-	chr4.4:82647617-82647636	None:intergenic	35.0%
!	ACTGATGATTGGTCATCATT+AGG	-	chr4.4:82646728-82646747	None:intergenic	35.0%
!	ATTCTAGAGAAAGTTGCTGT+TGG	+	chr4.4:82645494-82645513	MS.gene010656:CDS	35.0%
!	CCATGCATTTTCAAACTTGA+AGG	-	chr4.4:82647854-82647873	None:intergenic	35.0%
!	CCTTCAAGTTTGAAAATGCA+TGG	+	chr4.4:82647851-82647870	MS.gene010656:CDS	35.0%
!	TTTTCATCCATGAATCCGAA+CGG	-	chr4.4:82645581-82645600	None:intergenic	35.0%
!!	CCAAGTCAACAAAGTTTTAG+CGG	+	chr4.4:82646270-82646289	MS.gene010656:intron	35.0%
!!	CGAACAGGGAATTTGATTTT+AGG	-	chr4.4:82647178-82647197	None:intergenic	35.0%
!!	TAGTCAGTAGCTTTGAACAT+GGG	-	chr4.4:82646758-82646777	None:intergenic	35.0%
!!	TCAAATGACGTTGATTCTCA+TGG	+	chr4.4:82645379-82645398	MS.gene010656:CDS	35.0%
!!	TCAACAAAGTTTTAGCGGAA+GGG	+	chr4.4:82646275-82646294	MS.gene010656:intron	35.0%
!!	TCTGTTCTAGACCTTTTCTT+AGG	-	chr4.4:82646986-82647005	None:intergenic	35.0%
!!!	GGAAAGTTTTGTTTTGTGTG+TGG	+	chr4.4:82645791-82645810	MS.gene010656:CDS	35.0%
	AAATCTACCTTCGTGTCTGA+TGG	+	chr4.4:82647507-82647526	MS.gene010656:CDS	40.0%
	AATAAGCCAGTTTGGTCTAG+TGG	-	chr4.4:82647578-82647597	None:intergenic	40.0%
	AATCTACCTTCGTGTCTGAT+GGG	+	chr4.4:82647508-82647527	MS.gene010656:CDS	40.0%
	ACCTATTCATTTGGCTACGT+TGG	+	chr4.4:82645322-82645341	MS.gene010656:CDS	40.0%
	ATGCTGATGAGAAAAAAGGG+AGG	+	chr4.4:82647551-82647570	MS.gene010656:CDS	40.0%
	ATGTCAAAGATCAGTCGGAA+CGG	+	chr4.4:82646132-82646151	MS.gene010656:CDS	40.0%
	CAAAAATTCTGGCAGTCGTA+AGG	+	chr4.4:82645967-82645986	MS.gene010656:CDS	40.0%
	CACCAACCTTAGAACATTGT+GGG	-	chr4.4:82646385-82646404	None:intergenic	40.0%
	CAGAAAAGACAAAGGAGTTG+CGG	+	chr4.4:82646838-82646857	MS.gene010656:CDS	40.0%
	CATCAAGCAGTGAGAAGAAA+TGG	+	chr4.4:82645864-82645883	MS.gene010656:CDS	40.0%
	CCCTGTTCGGTATTATAAAC+CGG	+	chr4.4:82647189-82647208	MS.gene010656:intron	40.0%
	CCGGTTTATAATACCGAACA+GGG	-	chr4.4:82647192-82647211	None:intergenic	40.0%
	CCTGTTAGAAATAGTAGAGG+TGG	+	chr4.4:82647298-82647317	MS.gene010656:intron	40.0%
	CTCCATGCTGATGAGAAAAA+AGG	+	chr4.4:82647547-82647566	MS.gene010656:CDS	40.0%
	GACGATGCCTATGAATACTA+CGG	+	chr4.4:82645529-82645548	MS.gene010656:CDS	40.0%
	GAGAGTTCGAGCTAAAATTG+CGG	+	chr4.4:82645685-82645704	MS.gene010656:CDS	40.0%
	GCACCTGTTAGAAATAGTAG+AGG	+	chr4.4:82647295-82647314	MS.gene010656:intron	40.0%
	GGATTCATGGATGAAAAGGT+GGG	+	chr4.4:82645584-82645603	MS.gene010656:CDS	40.0%
	GTTAGAAATAGTAGAGGTGG+AGG	+	chr4.4:82647301-82647320	MS.gene010656:intron	40.0%
	GTTCGGATTCATGGATGAAA+AGG	+	chr4.4:82645580-82645599	MS.gene010656:CDS	40.0%
	TATTCATTTGGCTACGTTGG+AGG	+	chr4.4:82645325-82645344	MS.gene010656:CDS	40.0%
	TCAAGCAGTGAGAAGAAATG+GGG	+	chr4.4:82645866-82645885	MS.gene010656:CDS	40.0%
	TCCAACGTAGCCAAATGAAT+AGG	-	chr4.4:82645326-82645345	None:intergenic	40.0%
	TCCGGTTTATAATACCGAAC+AGG	-	chr4.4:82647193-82647212	None:intergenic	40.0%
	TCGATGAGGAAAACAACTAC+GGG	+	chr4.4:82645645-82645664	MS.gene010656:CDS	40.0%
	TGAAAATGCATGGAGAATCG+AGG	+	chr4.4:82647861-82647880	MS.gene010656:CDS	40.0%
	TGCAGAATTTGTCTCCACAA+AGG	+	chr4.4:82646781-82646800	MS.gene010656:CDS	40.0%
	TGCTGATGAGAAAAAAGGGA+GGG	+	chr4.4:82647552-82647571	MS.gene010656:CDS	40.0%
	TGGTCTGACTTTGTAAGGTT+TGG	-	chr4.4:82646253-82646272	None:intergenic	40.0%
	TGTTCTTGACCTCTTCTTGA+GGG	-	chr4.4:82646213-82646232	None:intergenic	40.0%
	TTGCTTGATCTTTGCCTTGT+TGG	+	chr4.4:82645281-82645300	MS.gene010656:CDS	40.0%
	TTGTGGAGACAAATTCTGCA+CGG	-	chr4.4:82646781-82646800	None:intergenic	40.0%
	TTGTTCTTGACCTCTTCTTG+AGG	-	chr4.4:82646214-82646233	None:intergenic	40.0%
!	AACTTTTCTTCTACTGCACG+CGG	-	chr4.4:82647691-82647710	None:intergenic	40.0%
!	AATTTTCGGTAAGCACTTGC+AGG	-	chr4.4:82646611-82646630	None:intergenic	40.0%
!	ACAAAAGAAGCATGGGCAAT+TGG	+	chr4.4:82646496-82646515	MS.gene010656:CDS	40.0%
!	ACAATACTCATTGGTCTCGT+CGG	-	chr4.4:82647115-82647134	None:intergenic	40.0%
!	AGACCAAACTGGCTTATTAG+AGG	+	chr4.4:82647580-82647599	MS.gene010656:CDS	40.0%
!	ATTGTGGGCTTCCTTTTTGT+TGG	-	chr4.4:82646370-82646389	None:intergenic	40.0%
!	CCGCTAAAACTTTGTTGACT+TGG	-	chr4.4:82646273-82646292	None:intergenic	40.0%
!	GACCAATGAGTATTGTAGCA+TGG	+	chr4.4:82647119-82647138	MS.gene010656:intron	40.0%
!	GTATTCATAGGCATCGTCAT+AGG	-	chr4.4:82645527-82645546	None:intergenic	40.0%
!	TGTTTTGTGTGTGGTGTGAT+AGG	+	chr4.4:82645800-82645819	MS.gene010656:CDS	40.0%
!	TTGTGGGCTTCCTTTTTGTT+GGG	-	chr4.4:82646369-82646388	None:intergenic	40.0%
!!	AGTCAGTAGCTTTGAACATG+GGG	-	chr4.4:82646757-82646776	None:intergenic	40.0%
!!	CCACCTCTACTATTTCTAAC+AGG	-	chr4.4:82647301-82647320	None:intergenic	40.0%
!!	CGGTCTTATTGATGTTCACA+TGG	+	chr4.4:82647627-82647646	MS.gene010656:CDS	40.0%
!!	GTAGTCAGTAGCTTTGAACA+TGG	-	chr4.4:82646759-82646778	None:intergenic	40.0%
!!	GTCAACAAAGTTTTAGCGGA+AGG	+	chr4.4:82646274-82646293	MS.gene010656:intron	40.0%
!!	TGACTTGGTCTGACTTTGTA+AGG	-	chr4.4:82646258-82646277	None:intergenic	40.0%
!!	TGATTTTAGGAATTACGCGC+AGG	-	chr4.4:82647165-82647184	None:intergenic	40.0%
!!!	GTGGAGGTATTGCTTTGTTT+TGG	+	chr4.4:82647317-82647336	MS.gene010656:CDS	40.0%
!!!	TCCCTTTTTTCTCATCAGCA+TGG	-	chr4.4:82647552-82647571	None:intergenic	40.0%
	AAATCTCCCATCAGACACGA+AGG	-	chr4.4:82647517-82647536	None:intergenic	45.0%
	AAATGCATGGAGAATCGAGG+AGG	+	chr4.4:82647864-82647883	MS.gene010656:CDS	45.0%
	AAGAAGTCAATCGTAGGGCT+TGG	+	chr4.4:82647401-82647420	MS.gene010656:CDS	45.0%
	AATGCATGGAGAATCGAGGA+GGG	+	chr4.4:82647865-82647884	MS.gene010656:CDS	45.0%
	AATTCAGCCTGAGTGAGGTT+CGG	-	chr4.4:82645263-82645282	None:intergenic	45.0%
	ACCAATGTGACTACCAACGA+GGG	-	chr4.4:82645612-82645631	None:intergenic	45.0%
	ACGAGAAGGACGAATGCTAA+TGG	+	chr4.4:82646302-82646321	MS.gene010656:intron	45.0%
	ACGTCAGCACAAAAGAAGCA+TGG	+	chr4.4:82646488-82646507	MS.gene010656:CDS	45.0%
	AGAAAAGTTGGATCGAGCCT+TGG	+	chr4.4:82647702-82647721	MS.gene010656:CDS	45.0%
	AGAAGGACGAATGCTAATGG+AGG	+	chr4.4:82646305-82646324	MS.gene010656:intron	45.0%
	AGAGCTGCCAGAAAAGACAA+AGG	+	chr4.4:82646830-82646849	MS.gene010656:CDS	45.0%
	AGGAAGCCCACAATGTTCTA+AGG	+	chr4.4:82646376-82646395	MS.gene010656:CDS	45.0%
	AGTTCGAGCTAAAATTGCGG+TGG	+	chr4.4:82645688-82645707	MS.gene010656:CDS	45.0%
	ATGAGGTAGTCCAAGATAGC+TGG	+	chr4.4:82647893-82647912	MS.gene010656:CDS	45.0%
	ATTGTAGCATGGAATTGCCG+AGG	+	chr4.4:82647130-82647149	MS.gene010656:intron	45.0%
	CACCTGTAGTGTCTTCCATA+GGG	-	chr4.4:82646186-82646205	None:intergenic	45.0%
	CAGGTACACCAATTACCGTT+CGG	+	chr4.4:82645563-82645582	MS.gene010656:CDS	45.0%
	CATCCAAGAGTTAGTGACCA+AGG	-	chr4.4:82647722-82647741	None:intergenic	45.0%
	CCAATTACCGTTCGGATTCA+TGG	+	chr4.4:82645571-82645590	MS.gene010656:CDS	45.0%
	CCACCAACCTTAGAACATTG+TGG	-	chr4.4:82646386-82646405	None:intergenic	45.0%
	CCATGAATCCGAACGGTAAT+TGG	-	chr4.4:82645574-82645593	None:intergenic	45.0%
	CGAACTTCGATCAAGGATTC+TGG	-	chr4.4:82645450-82645469	None:intergenic	45.0%
	CGAGAAGCCATTCAAGAAGT+CGG	+	chr4.4:82647607-82647626	MS.gene010656:CDS	45.0%
	CGGATTCATGGATGAAAAGG+TGG	+	chr4.4:82645583-82645602	MS.gene010656:CDS	45.0%
	CTATGGAAGACACTACAGGT+GGG	+	chr4.4:82646185-82646204	MS.gene010656:CDS	45.0%
	GAATCCTTGATCGAAGTTCG+TGG	+	chr4.4:82645450-82645469	MS.gene010656:CDS	45.0%
	GCGTGCAGTAGAAGAAAAGT+TGG	+	chr4.4:82647690-82647709	MS.gene010656:CDS	45.0%
	GCTGACGATTGCTGCAATAA+TGG	-	chr4.4:82646578-82646597	None:intergenic	45.0%
	GGAGGGGCAGAAAATATTGT+CGG	+	chr4.4:82647033-82647052	MS.gene010656:intron	45.0%
	GGTTCAGCTGTATTCAAAGG+CGG	+	chr4.4:82645418-82645437	MS.gene010656:CDS	45.0%
	GTATACGGGCGCATTAAAGT+AGG	+	chr4.4:82646064-82646083	MS.gene010656:CDS	45.0%
	GTGCAGAAGATCTAACACAC+TGG	+	chr4.4:82647959-82647978	MS.gene010656:intron	45.0%
	GTTCTTGACCTCTTCTTGAG+GGG	-	chr4.4:82646212-82646231	None:intergenic	45.0%
	TAACCATCACTCGACCAACA+AGG	-	chr4.4:82645298-82645317	None:intergenic	45.0%
	TACCAATGTGACTACCAACG+AGG	-	chr4.4:82645613-82645632	None:intergenic	45.0%
	TATCTTGCACGACAGAACCT+CGG	+	chr4.4:82647801-82647820	MS.gene010656:CDS	45.0%
	TATGGAAGACACTACAGGTG+GGG	+	chr4.4:82646186-82646205	MS.gene010656:CDS	45.0%
	TCTATGATTTCGAGCTACCG+AGG	-	chr4.4:82647821-82647840	None:intergenic	45.0%
	TGAAGAACGTGACGTTGGAA+AGG	+	chr4.4:82647012-82647031	MS.gene010656:CDS	45.0%
	TGAGGTAGTCCAAGATAGCT+GGG	+	chr4.4:82647894-82647913	MS.gene010656:CDS	45.0%
	TGTCAAAGATCAGTCGGAAC+GGG	+	chr4.4:82646133-82646152	MS.gene010656:CDS	45.0%
	TTCGATCAAGGATTCTGGTC+AGG	-	chr4.4:82645445-82645464	None:intergenic	45.0%
	TTTGCTGAAGAACGTGACGT+TGG	+	chr4.4:82647007-82647026	MS.gene010656:CDS	45.0%
!	ACTTTTATGGCTAGTGCACG+TGG	+	chr4.4:82646923-82646942	MS.gene010656:CDS	45.0%
!	AGAGAAAGTTGCTGTTGGAG+AGG	+	chr4.4:82645499-82645518	MS.gene010656:CDS	45.0%
!	ATGTTGGTCTGCAGACTGAT+GGG	-	chr4.4:82646537-82646556	None:intergenic	45.0%
!	CAATACTCATTGGTCTCGTC+GGG	-	chr4.4:82647114-82647133	None:intergenic	45.0%
!	CGCAACTCCTTTGTCTTTTC+TGG	-	chr4.4:82646840-82646859	None:intergenic	45.0%
!	CGTCAGCACAAAAGAAGCAT+GGG	+	chr4.4:82646489-82646508	MS.gene010656:CDS	45.0%
!	CTTTTATGGCTAGTGCACGT+GGG	+	chr4.4:82646924-82646943	MS.gene010656:CDS	45.0%
!	TTTTATGGCTAGTGCACGTG+GGG	+	chr4.4:82646925-82646944	MS.gene010656:CDS	45.0%
!!	AGCCCACAATGTTCTAAGGT+TGG	+	chr4.4:82646380-82646399	MS.gene010656:CDS	45.0%
!!	CCACAATGTTCTAAGGTTGG+TGG	+	chr4.4:82646383-82646402	MS.gene010656:CDS	45.0%
!!	GTCAGTAGCTTTGAACATGG+GGG	-	chr4.4:82646756-82646775	None:intergenic	45.0%
!!	TTTGTCTTTTCTGGCAGCTC+TGG	-	chr4.4:82646831-82646850	None:intergenic	45.0%
!!!	TGGAGGGGTATCCTTTTACT+TGG	+	chr4.4:82647647-82647666	MS.gene010656:CDS	45.0%
	AAAAATGAAACGGCCGGAGC+TGG	+	chr4.4:82647080-82647099	MS.gene010656:intron	50.0%
	AAAACAACTACGGGCCATGG+CGG	+	chr4.4:82645654-82645673	MS.gene010656:CDS	50.0%
	AAAGCAGCTAGCCGACAGTA+GGG	+	chr4.4:82646445-82646464	MS.gene010656:CDS	50.0%
	AAAGCTGAGAACAACGCCGT+TGG	+	chr4.4:82645899-82645918	MS.gene010656:CDS	50.0%
	AACAGGAGCTGGTCATTGAG+AGG	+	chr4.4:82645723-82645742	MS.gene010656:CDS	50.0%
	AACGTGACGTTGGAAAGGAG+GGG	+	chr4.4:82647017-82647036	MS.gene010656:CDS	50.0%
	ACAGGAGCTGGTCATTGAGA+GGG	+	chr4.4:82645724-82645743	MS.gene010656:CDS	50.0%
	ACTCTCATAAACCTCCGCCA+TGG	-	chr4.4:82645671-82645690	None:intergenic	50.0%
	AGAACGTGACGTTGGAAAGG+AGG	+	chr4.4:82647015-82647034	MS.gene010656:CDS	50.0%
	AGGAAAACAACTACGGGCCA+TGG	+	chr4.4:82645651-82645670	MS.gene010656:CDS	50.0%
	AGGAAGCTGGGCCTAAGAAA+AGG	+	chr4.4:82646972-82646991	MS.gene010656:CDS	50.0%
	AGGACGAATGCTAATGGAGG+AGG	+	chr4.4:82646308-82646327	MS.gene010656:intron	50.0%
	ATGACCAGCTCCTGTTGTAG+TGG	-	chr4.4:82645719-82645738	None:intergenic	50.0%
	ATGCATGGAGAATCGAGGAG+GGG	+	chr4.4:82647866-82647885	MS.gene010656:CDS	50.0%
	CAAGCAATTCAGCCTGAGTG+AGG	-	chr4.4:82645268-82645287	None:intergenic	50.0%
	CAGTCGTAAGGAAGAAGGAG+CGG	+	chr4.4:82645979-82645998	MS.gene010656:CDS	50.0%
	CCACCTGTAGTGTCTTCCAT+AGG	-	chr4.4:82646187-82646206	None:intergenic	50.0%
	CCTATGGAAGACACTACAGG+TGG	+	chr4.4:82646184-82646203	MS.gene010656:CDS	50.0%
	CGAACGGTAATTGGTGTACC+TGG	-	chr4.4:82645565-82645584	None:intergenic	50.0%
	CGTAGGGCTTGGATATTCAC+AGG	+	chr4.4:82647412-82647431	MS.gene010656:CDS	50.0%
	GAACGTGACGTTGGAAAGGA+GGG	+	chr4.4:82647016-82647035	MS.gene010656:CDS	50.0%
	GAAGCAAGCAAGTGGATCGT+CGG	+	chr4.4:82645935-82645954	MS.gene010656:CDS	50.0%
	GACCCTATGGAAGACACTAC+AGG	+	chr4.4:82646181-82646200	MS.gene010656:CDS	50.0%
	GAGCCTTGGTCACTAACTCT+TGG	+	chr4.4:82647716-82647735	MS.gene010656:CDS	50.0%
	GAGGTTCTGTCGTGCAAGAT+AGG	-	chr4.4:82647802-82647821	None:intergenic	50.0%
	GCAACAAGGAAGCAAGCAAG+TGG	+	chr4.4:82645927-82645946	MS.gene010656:CDS	50.0%
	GCAACCACGAACTTCGATCA+AGG	-	chr4.4:82645457-82645476	None:intergenic	50.0%
	GCAATTGGTCCTGTTCGATG+GGG	+	chr4.4:82646511-82646530	MS.gene010656:CDS	50.0%
	GGCAATTGGTCCTGTTCGAT+GGG	+	chr4.4:82646510-82646529	MS.gene010656:CDS	50.0%
	GGTGGTCGGAGCAAAAATTC+TGG	+	chr4.4:82645956-82645975	MS.gene010656:CDS	50.0%
	GTAGTCCAAGATAGCTGGGT+CGG	+	chr4.4:82647898-82647917	MS.gene010656:CDS	50.0%
	TAATGCGCCCGTATACCTTG+TGG	-	chr4.4:82646060-82646079	None:intergenic	50.0%
	TACTACGGAGATTTGGGTCC+AGG	+	chr4.4:82645544-82645563	MS.gene010656:CDS	50.0%
	TAGTCCAAGATAGCTGGGTC+GGG	+	chr4.4:82647899-82647918	MS.gene010656:CDS	50.0%
	TCGAGCTAAAATTGCGGTGG+AGG	+	chr4.4:82645691-82645710	MS.gene010656:CDS	50.0%
	TCTGGCAGTCGTAAGGAAGA+AGG	+	chr4.4:82645974-82645993	MS.gene010656:CDS	50.0%
	TGGGGGACAAGACTGATGAT+TGG	-	chr4.4:82646739-82646758	None:intergenic	50.0%
	TGTCGAAATGGACCTCATCC+TGG	-	chr4.4:82648068-82648087	None:intergenic	50.0%
	TTCCTTGTTGCCACCACCAA+CGG	-	chr4.4:82645918-82645937	None:intergenic	50.0%
!	AGTGCAGCCTGGCACTTTTA+TGG	+	chr4.4:82646910-82646929	MS.gene010656:CDS	50.0%
!	CATGTTGGTCTGCAGACTGA+TGG	-	chr4.4:82646538-82646557	None:intergenic	50.0%
!	CTGGCTTGAGTTGTCCTTTG+TGG	-	chr4.4:82646798-82646817	None:intergenic	50.0%
!	TTGCCTTGTTGGTCGAGTGA+TGG	+	chr4.4:82645292-82645311	MS.gene010656:CDS	50.0%
!!	AACATGGTGCTGCATCCAAG+AGG	+	chr4.4:82645237-82645256	MS.gene010656:CDS	50.0%
!!	ATAAAAGTGCCAGGCTGCAC+TGG	-	chr4.4:82646911-82646930	None:intergenic	50.0%
!!	GAGGAGGTGCAGAAGCATTA+TGG	+	chr4.4:82646324-82646343	MS.gene010656:intron	50.0%
!!	GCACTAGCCATAAAAGTGCC+AGG	-	chr4.4:82646920-82646939	None:intergenic	50.0%
!!	TAAAAGTGCCAGGCTGCACT+GGG	-	chr4.4:82646910-82646929	None:intergenic	50.0%
	AATCGAGGAGGGGGTGAATG+AGG	+	chr4.4:82647876-82647895	MS.gene010656:CDS	55.0%
	ACGTCATGCTGTCCACAGGT+GGG	-	chr4.4:82646472-82646491	None:intergenic	55.0%
	ACTGGGTTGCGAGCTATGAC+TGG	-	chr4.4:82646893-82646912	None:intergenic	55.0%
	AGCATGGAATTGCCGAGGCT+TGG	+	chr4.4:82647135-82647154	MS.gene010656:intron	55.0%
	AGCGGAAGGGATGAACGAGA+AGG	+	chr4.4:82646288-82646307	MS.gene010656:intron	55.0%
	ATTTGGCTACGTTGGAGGCG+AGG	+	chr4.4:82645330-82645349	MS.gene010656:CDS	55.0%
	CTCATCCTGGATTCCGTGAG+CGG	-	chr4.4:82648055-82648074	None:intergenic	55.0%
	CTGGTCATTGAGAGGGAGGA+AGG	+	chr4.4:82645731-82645750	MS.gene010656:CDS	55.0%
	GAAAGCAGCTAGCCGACAGT+AGG	+	chr4.4:82646444-82646463	MS.gene010656:CDS	55.0%
	GCATGGAATTGCCGAGGCTT+GGG	+	chr4.4:82647136-82647155	MS.gene010656:intron	55.0%
	GGGCAATTGGTCCTGTTCGA+TGG	+	chr4.4:82646509-82646528	MS.gene010656:CDS	55.0%
	GGTACCACTACAACAGGAGC+TGG	+	chr4.4:82645712-82645731	MS.gene010656:CDS	55.0%
	TGCATGGAGAATCGAGGAGG+GGG	+	chr4.4:82647867-82647886	MS.gene010656:CDS	55.0%
	TTAGGCCCAGCTTCCTCTTG+AGG	-	chr4.4:82646968-82646987	None:intergenic	55.0%
!!	CTTTTCTGGCAGCTCTGGCT+CGG	-	chr4.4:82646826-82646845	None:intergenic	55.0%
!!	GCCCTCGTTGGTAGTCACAT+TGG	+	chr4.4:82645608-82645627	MS.gene010656:CDS	55.0%
!!	GGTGGAGGTACCACTACAAC+AGG	+	chr4.4:82645706-82645725	MS.gene010656:CDS	55.0%
	AGTAGAAGCCGCACCGCTCA+CGG	+	chr4.4:82648039-82648058	MS.gene010656:CDS	60.0%
	CAAGAGGCCGAACCTCACTC+AGG	+	chr4.4:82645253-82645272	MS.gene010656:CDS	60.0%
	CACAGGTGGGACCCTACTGT+CGG	-	chr4.4:82646459-82646478	None:intergenic	60.0%
	CACTCCCGACCCAGCTATCT+TGG	-	chr4.4:82647906-82647925	None:intergenic	60.0%
	CGCTCACGGAATCCAGGATG+AGG	+	chr4.4:82648053-82648072	MS.gene010656:CDS	60.0%
	CTGAGTGAGGTTCGGCCTCT+TGG	-	chr4.4:82645255-82645274	None:intergenic	60.0%
	GAAAAGGTGGGTGCCCTCGT+TGG	+	chr4.4:82645596-82645615	MS.gene010656:CDS	60.0%
	GAACGGGCGTAGTGAGATGG+TGG	+	chr4.4:82646149-82646168	MS.gene010656:CDS	60.0%
	GACGTCATGCTGTCCACAGG+TGG	-	chr4.4:82646473-82646492	None:intergenic	60.0%
	GATGTAGCGCGTGGCACAAG+TGG	+	chr4.4:82646413-82646432	MS.gene010656:CDS	60.0%
	GCAAGCAAGTGGATCGTCGG+TGG	+	chr4.4:82645938-82645957	MS.gene010656:CDS	60.0%
	GCAAGTGGATCGTCGGTGGT+CGG	+	chr4.4:82645942-82645961	MS.gene010656:CDS	60.0%
	GCTGACGTCATGCTGTCCAC+AGG	-	chr4.4:82646476-82646495	None:intergenic	60.0%
	GCTGAGAACAACGCCGTTGG+TGG	+	chr4.4:82645902-82645921	MS.gene010656:CDS	60.0%
	GGAGCTGGTCATTGAGAGGG+AGG	+	chr4.4:82645727-82645746	MS.gene010656:CDS	60.0%
	GGAGGGCCACTAGACCAAAC+TGG	+	chr4.4:82647569-82647588	MS.gene010656:CDS	60.0%
	GGCAGCAATGATGTAGCGCG+TGG	+	chr4.4:82646404-82646423	MS.gene010656:CDS	60.0%
	GTGGACGGAGATCGACCCTA+TGG	+	chr4.4:82646168-82646187	MS.gene010656:CDS	60.0%
	GTGGGCCTCAAGAGGAAGCT+GGG	+	chr4.4:82646960-82646979	MS.gene010656:CDS	60.0%
	TCGGAACGGGCGTAGTGAGA+TGG	+	chr4.4:82646146-82646165	MS.gene010656:CDS	60.0%
	TGGCTTCCCATGCTGCTGGA+TGG	-	chr4.4:82646864-82646883	None:intergenic	60.0%
	TTGAGGCCCACCAGCTCTAC+TGG	-	chr4.4:82646951-82646970	None:intergenic	60.0%
	TTGCGGCCATCCAGCAGCAT+GGG	+	chr4.4:82646855-82646874	MS.gene010656:CDS	60.0%
!	ACTGATGGGCCCCATCGAAC+AGG	-	chr4.4:82646523-82646542	None:intergenic	60.0%
!	AGGCGAGGCTCGGTCCTATT+TGG	+	chr4.4:82645345-82645364	MS.gene010656:CDS	60.0%
!	CTCATTGGTCTCGTCGGGCT+TGG	-	chr4.4:82647109-82647128	None:intergenic	60.0%
!	GAGAACAACGCCGTTGGTGG+TGG	+	chr4.4:82645905-82645924	MS.gene010656:CDS	60.0%
!	GGCGAGGCTCGGTCCTATTT+GGG	+	chr4.4:82645346-82645365	MS.gene010656:CDS	60.0%
!	TAGAGCTGGTGGGCCTCAAG+AGG	+	chr4.4:82646952-82646971	MS.gene010656:CDS	60.0%
!!	CGAGCTATGACTGGCTTGCG+TGG	-	chr4.4:82646884-82646903	None:intergenic	60.0%
!!	GGGCGTAGTGAGATGGTGGA+CGG	+	chr4.4:82646153-82646172	MS.gene010656:CDS	60.0%
	ACAACTACGGGCCATGGCGG+AGG	+	chr4.4:82645657-82645676	MS.gene010656:CDS	65.0%
	AGCGCGTGGCACAAGTGGTG+AGG	+	chr4.4:82646418-82646437	MS.gene010656:CDS	65.0%
	AGGTGGGGCCCCTCAAGAAG+AGG	+	chr4.4:82646201-82646220	MS.gene010656:CDS	65.0%
	CCTGGATTCCGTGAGCGGTG+CGG	-	chr4.4:82648050-82648069	None:intergenic	65.0%
	CGACAGTAGGGTCCCACCTG+TGG	+	chr4.4:82646457-82646476	MS.gene010656:CDS	65.0%
	CGCGCAGGTTTCCCAAGCCT+CGG	-	chr4.4:82647150-82647169	None:intergenic	65.0%
	CGTGGCACAAGTGGTGAGGG+TGG	+	chr4.4:82646422-82646441	MS.gene010656:CDS	65.0%
	GCGCGTGGCACAAGTGGTGA+GGG	+	chr4.4:82646419-82646438	MS.gene010656:CDS	65.0%
	GCTACGTTGGAGGCGAGGCT+CGG	+	chr4.4:82645335-82645354	MS.gene010656:CDS	65.0%
	GGTGGGCCTCAAGAGGAAGC+TGG	+	chr4.4:82646959-82646978	MS.gene010656:CDS	65.0%
	GTGGGGCCAGTAGAGCTGGT+GGG	+	chr4.4:82646942-82646961	MS.gene010656:CDS	65.0%
	GTTGCGGCCATCCAGCAGCA+TGG	+	chr4.4:82646854-82646873	MS.gene010656:CDS	65.0%
	TGCGTGGCTTCCCATGCTGC+TGG	-	chr4.4:82646868-82646887	None:intergenic	65.0%
!!	CGCCGTTGGTGGTGGCAACA+AGG	+	chr4.4:82645913-82645932	MS.gene010656:CDS	65.0%
	CCGCACCGCTCACGGAATCC+AGG	+	chr4.4:82648047-82648066	MS.gene010656:CDS	70.0%
	CGTGGGGCCAGTAGAGCTGG+TGG	+	chr4.4:82646941-82646960	MS.gene010656:CDS	70.0%
	GCACGTGGGGCCAGTAGAGC+TGG	+	chr4.4:82646938-82646957	MS.gene010656:CDS	70.0%
	GCTCGCAACCCAGTGCAGCC+TGG	+	chr4.4:82646899-82646918	MS.gene010656:CDS	70.0%
	TCGGGCTTGGCGACCAGCTC+CGG	-	chr4.4:82647096-82647115	None:intergenic	70.0%
!!	CAGCTCTGGCTCGGCTTGGC+TGG	-	chr4.4:82646817-82646836	None:intergenic	70.0%
!!	CTGGCAGCTCTGGCTCGGCT+TGG	-	chr4.4:82646821-82646840	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	82645212	82648093	82645212	ID=MS.gene010656
chr4.4	mRNA	82645212	82648093	82645212	ID=MS.gene010656.t1;Parent=MS.gene010656
chr4.4	exon	82645212	82646273	82645212	ID=MS.gene010656.t1.exon1;Parent=MS.gene010656.t1
chr4.4	CDS	82645212	82646273	82645212	ID=cds.MS.gene010656.t1;Parent=MS.gene010656.t1
chr4.4	exon	82646365	82647050	82646365	ID=MS.gene010656.t1.exon2;Parent=MS.gene010656.t1
chr4.4	CDS	82646365	82647050	82646365	ID=cds.MS.gene010656.t1;Parent=MS.gene010656.t1
chr4.4	exon	82647306	82647922	82647306	ID=MS.gene010656.t1.exon3;Parent=MS.gene010656.t1
chr4.4	CDS	82647306	82647922	82647306	ID=cds.MS.gene010656.t1;Parent=MS.gene010656.t1
chr4.4	exon	82648029	82648093	82648029	ID=MS.gene010656.t1.exon4;Parent=MS.gene010656.t1
chr4.4	CDS	82648029	82648093	82648029	ID=cds.MS.gene010656.t1;Parent=MS.gene010656.t1

Gene Sequence

>MS.gene010656

ATGGAGAGACTTAACATCAATGGTGAACATGGTGCTGCATCCAAGAGGCCGAACCTCACTCAGGCTGAATTGCTTGATCTTTGCCTTGTTGGTCGAGTGATGGTTAACAAACCTATTCATTTGGCTACGTTGGAGGCGAGGCTCGGTCCTATTTGGGAACCTAAGTATCAAATGACGTTGATTCTCATGGAAGACAACAAGTTTATGGTTCAGCTGTATTCAAAGGCGGACCTGACCAGAATCCTTGATCGAAGTTCGTGGTTGCTCGATAACAATATGATCATTCTAGAGAAAGTTGCTGTTGGAGAGGATCCTATGACGATGCCTATGAATACTACGGAGATTTGGGTCCAGGTACACCAATTACCGTTCGGATTCATGGATGAAAAGGTGGGTGCCCTCGTTGGTAGTCACATTGGTAAAATGATTAGGTTCGATGAGGAAAACAACTACGGGCCATGGCGGAGGTTTATGAGAGTTCGAGCTAAAATTGCGGTGGAGGTACCACTACAACAGGAGCTGGTCATTGAGAGGGAGGAAGGTGAAAACATTAAACTTGTTTTCAAGTATGAGAAATTGGGAAAGTTTTGTTTTGTGTGTGGTGTGATAGGTCACTCAGAAAACTTTTGCAGTGACAAGTTTGAATCTGCTTCATCAAGCAGTGAGAAGAAATGGGGTTCATATCTCAAAGCTGAGAACAACGCCGTTGGTGGTGGCAACAAGGAAGCAAGCAAGTGGATCGTCGGTGGTCGGAGCAAAAATTCTGGCAGTCGTAAGGAAGAAGGAGCGGCCATCAATGACATTCAAAGTCATTCATTGGCATTCAATGATAACGGAATTTCAAACCACAAGGTATACGGGCGCATTAAAGTAGGTATTAATGCAGAGACAAGGGCTTTAACTTTCTTTAAGTATATGGAATGTCAAAGATCAGTCGGAACGGGCGTAGTGAGATGGTGGACGGAGATCGACCCTATGGAAGACACTACAGGTGGGGCCCCTCAAGAAGAGGTCAAGAACAATTCTAAAGTGTTTGCTCCAAACCTTACAAAGTCAGACCAAGTCAACAAAGTTTTAGCGGAAGGGATGAACGAGAAGGACGAATGCTAATGGAGGAGGTGCAGAAGCATTATGGAAGTTGATATATTTAGAGGCCCAACAAAAAGGAAGCCCACAATGTTCTAAGGTTGGTGGCAGCAATGATGTAGCGCGTGGCACAAGTGGTGAGGGTGGAAAGCAGCTAGCCGACAGTAGGGTCCCACCTGTGGACAGCATGACGTCAGCACAAAAGAAGCATGGGCAATTGGTCCTGTTCGATGGGGCCCATCAGTCTGCAGACCAACATGTCAAACAATCATTAAGACCATTATTGCAGCAATCGTCAGCAATAAAAATGCCTGCAAGTGCTTACCGAAAATTGTTTTCAAAGGAGATAGCACCAATAAATGTCTCTTTCAATGCTAATAATGGTGTTTTTCAGGTTCAAAAAGTTGACAACTTTCAACATTTATTGCCTAATGATGACCAATCATCAGTCTTGTCCCCCATGTTCAAAGCTACTGACTACCGTGCAGAATTTGTCTCCACAAAGGACAACTCAAGCCAGCCAAGCCGAGCCAGAGCTGCCAGAAAAGACAAAGGAGTTGCGGCCATCCAGCAGCATGGGAAGCCACGCAAGCCAGTCATAGCTCGCAACCCAGTGCAGCCTGGCACTTTTATGGCTAGTGCACGTGGGGCCAGTAGAGCTGGTGGGCCTCAAGAGGAAGCTGGGCCTAAGAAAAGGTCTAGAACAGAATTTGCTGAAGAACGTGACGTTGGAAAGGAGGGGCAGAAAATATTGTCGGTCATAGATAACCCAATTTTTGAAAAAAAATGAAACGGCCGGAGCTGGTCGCCAAGCCCGACGAGACCAATGAGTATTGTAGCATGGAATTGCCGAGGCTTGGGAAACCTGCGCGTAATTCCTAAAATCAAATTCCCTGTTCGGTATTATAAACCGGATATCCTTTTTCTTTGTGAAACTATTGTTGAAGCTAATAAAATCGAGGAATTTCATTATTTATTGGGTTATGATTCTTGTTTTGCACCTGTTAGAAATAGTAGAGGTGGAGGTATTGCTTTGTTTTGGAGAAATACGGTTAACTGTAGTATTGTTAATTATTCAGCTAACCATATTAGAGCTAAAATTGAAGAAGTCAATCGTAGGGCTTGGATATTCACAGGATTTTATGGATTTCCAAAAATAGGAAGAAGAAGAGATTCTTGGAATTTTATTCTTGGGCTAGCTAGACAGATAAATCTACCTTCGTGTCTGATGGGAGATTTTAACGATATACTCCATGCTGATGAGAAAAAAGGGAGGGCCACTAGACCAAACTGGCTTATTAGAGGATTTCGAGAAGCCATTCAAGAAGTCGGTCTTATTGATGTTCACATGGAGGGGTATCCTTTTACTTGGTTTAAAAGTTTGGGAACTCCGCGTGCAGTAGAAGAAAAGTTGGATCGAGCCTTGGTCACTAACTCTTGGATGCAGTTATTTCCTAATGCCAAATTAGAAAATCTTGTTGCTCCATCATCAGATCAATTTCCTATCTTGCACGACAGAACCTCGGTAGCTCGAAATCATAGAGTAGAAAGGTCCTTCAAGTTTGAAAATGCATGGAGAATCGAGGAGGGGGTGAATGAGGTAGTCCAAGATAGCTGGGTCGGGAGTGCAGAAAGCAATGTCATCAATAAATTATCAATTTGTGCAGAAGATCTAACACACTGGAGTAAAACCCATTGTAATAAGATTCAAGAAGAGATTGATCATTGCAGGAAACAACTCAGTAGAAGCCGCACCGCTCACGGAATCCAGGATGAGGTCCATTTCGACAATATGA

Protein sequence

>MS.gene010656.t1

MERLNINGEHGAASKRPNLTQAELLDLCLVGRVMVNKPIHLATLEARLGPIWEPKYQMTLILMEDNKFMVQLYSKADLTRILDRSSWLLDNNMIILEKVAVGEDPMTMPMNTTEIWVQVHQLPFGFMDEKVGALVGSHIGKMIRFDEENNYGPWRRFMRVRAKIAVEVPLQQELVIEREEGENIKLVFKYEKLGKFCFVCGVIGHSENFCSDKFESASSSSEKKWGSYLKAENNAVGGGNKEASKWIVGGRSKNSGSRKEEGAAINDIQSHSLAFNDNGISNHKVYGRIKVGINAETRALTFFKYMECQRSVGTGVVRWWTEIDPMEDTTGGAPQEEVKNNSKVFAPNLTKSDQAQQKGSPQCSKVGGSNDVARGTSGEGGKQLADSRVPPVDSMTSAQKKHGQLVLFDGAHQSADQHVKQSLRPLLQQSSAIKMPASAYRKLFSKEIAPINVSFNANNGVFQVQKVDNFQHLLPNDDQSSVLSPMFKATDYRAEFVSTKDNSSQPSRARAARKDKGVAAIQQHGKPRKPVIARNPVQPGTFMASARGASRAGGPQEEAGPKKRSRTEFAEERDVGKEGQKILNSRGGGIALFWRNTVNCSIVNYSANHIRAKIEEVNRRAWIFTGFYGFPKIGRRRDSWNFILGLARQINLPSCLMGDFNDILHADEKKGRATRPNWLIRGFREAIQEVGLIDVHMEGYPFTWFKSLGTPRAVEEKLDRALVTNSWMQLFPNAKLENLVAPSSDQFPILHDRTSVARNHRVERSFKFENAWRIEEGVNEVVQDSWVGRNNSVEAAPLTESRMRSISTI