AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30136

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30136.t1 MTR_3g053920 51.156 346 103 3 1 346 1 280 3.05e-106 325
MS.gene30136.t1 MTR_2g061500 74.419 129 33 0 1 129 1 129 1.18e-64 211
MS.gene30136.t1 MTR_7g011710 82.353 85 15 0 55 139 1 85 1.41e-43 154
MS.gene30136.t1 MTR_4g103530 67.368 95 31 0 55 149 1 95 1.64e-39 144
MS.gene30136.t1 MTR_6g033215 33.708 178 116 1 24 199 117 294 2.69e-32 127
MS.gene30136.t1 MTR_1g054905 63.415 82 30 0 495 576 58 139 1.74e-28 112
MS.gene30136.t1 MTR_6g046670 49.550 111 45 1 496 595 81 191 8.98e-26 105
MS.gene30136.t1 MTR_0010s0370 30.994 171 114 2 11 177 7 177 8.00e-24 99.8
MS.gene30136.t1 MTR_0010s0350 30.994 171 114 2 11 177 7 177 8.00e-24 99.8
MS.gene30136.t1 MTR_8g463110 59.211 76 31 0 408 483 219 294 2.82e-23 105
MS.gene30136.t1 MTR_8g463110 46.259 147 73 2 289 430 1 146 2.76e-22 102
MS.gene30136.t1 MTR_7g013520 56.790 81 32 1 496 576 31 108 2.90e-23 98.2
MS.gene30136.t1 MTR_6g034215 60.000 80 32 0 400 479 215 294 2.29e-22 99.4
MS.gene30136.t1 MTR_7g078610 48.889 90 37 1 486 575 8 88 3.74e-22 91.7
MS.gene30136.t1 MTR_4g020630 39.286 112 64 1 406 517 153 260 1.51e-20 96.3
MS.gene30136.t1 MTR_6g089560 56.338 71 30 1 506 575 83 153 8.05e-19 86.7
MS.gene30136.t1 MTR_2g047100 66.667 51 17 0 521 571 1 51 4.21e-18 80.5
MS.gene30136.t1 MTR_2g017550 62.712 59 22 0 517 575 87 145 2.73e-17 80.1
MS.gene30136.t1 MTR_1g017840 42.000 100 51 2 518 610 58 157 4.30e-17 79.7
MS.gene30136.t1 MTR_1g018300 61.017 59 23 0 516 574 41 99 6.22e-17 81.6
MS.gene30136.t1 MTR_8g099580 59.322 59 24 0 517 575 185 243 1.47e-16 81.6
MS.gene30136.t1 MTR_1g099430 44.578 83 37 2 504 586 95 168 5.74e-16 77.8
MS.gene30136.t1 MTR_5g036320 43.373 83 46 1 494 575 34 116 2.60e-15 73.9
MS.gene30136.t1 MTR_5g049680 50.000 62 31 0 514 575 7 68 3.40e-14 69.7
MS.gene30136.t1 MTR_4g029360 57.627 59 25 0 517 575 106 164 4.03e-14 72.8
MS.gene30136.t1 MTR_8g036780 54.237 59 27 0 517 575 13 71 7.20e-14 68.9
MS.gene30136.t1 MTR_6g016265 54.098 61 28 0 515 575 16 76 1.26e-13 68.6
MS.gene30136.t1 MTR_1964s0010 60.000 45 18 0 517 561 48 92 1.58e-13 67.4
MS.gene30136.t1 MTR_8g031080 40.449 89 52 1 491 579 243 330 2.22e-13 73.2
MS.gene30136.t1 MTR_2g027020 41.860 86 38 2 517 590 7 92 3.58e-13 68.9
MS.gene30136.t1 MTR_6g453270 45.122 82 43 1 496 575 256 337 3.64e-13 72.4
MS.gene30136.t1 MTR_5g068115 52.727 55 26 0 517 571 13 67 4.01e-13 65.1
MS.gene30136.t1 MTR_5g034450 58.491 53 22 0 523 575 34 86 4.47e-13 66.6
MS.gene30136.t1 MTR_1g036720 31.731 104 69 1 11 112 7 110 6.59e-13 65.9
MS.gene30136.t1 MTR_1g100687 42.029 69 40 0 408 476 157 225 1.02e-12 68.9
MS.gene30136.t1 MTR_1g074090 50.000 70 35 0 506 575 49 118 1.12e-12 66.6
MS.gene30136.t1 MTR_8g069135 65.116 43 15 0 517 559 168 210 1.45e-12 69.7
MS.gene30136.t1 MTR_3g462490 51.613 62 30 0 514 575 77 138 2.83e-12 69.7
MS.gene30136.t1 MTR_1g081930 49.153 59 30 0 517 575 17 75 3.33e-12 64.7
MS.gene30136.t1 MTR_1g034430 46.032 63 34 0 517 579 74 136 6.55e-12 65.1
MS.gene30136.t1 MTR_1g022215 54.000 50 23 0 526 575 2 51 9.18e-12 62.0
MS.gene30136.t1 MTR_4g046027 48.387 62 32 0 518 579 107 168 3.15e-11 62.8
MS.gene30136.t1 MTR_7g106180 24.476 143 103 2 36 175 26 166 5.39e-11 63.5
MS.gene30136.t1 MTR_2g102247 52.727 55 24 1 505 557 94 148 5.39e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30136.t1 AT2G17920 26.761 213 150 3 8 216 9 219 1.86e-18 87.4
MS.gene30136.t1 AT5G36228 28.125 192 134 2 36 225 39 228 6.00e-17 83.6
MS.gene30136.t1 AT2G41590 26.829 164 116 2 55 216 58 219 4.48e-16 81.3
MS.gene30136.t1 AT3G57587 26.606 218 144 7 8 216 9 219 1.01e-15 80.1
MS.gene30136.t1 AT2G02103 27.322 183 129 2 36 216 39 219 3.91e-15 78.6
MS.gene30136.t1 AT5G18636 26.776 183 130 2 36 216 39 219 4.75e-15 77.8
MS.gene30136.t1 AT5G25200 26.776 183 130 2 36 216 39 219 4.79e-15 77.8
MS.gene30136.t1 AT2G13450 27.869 183 126 4 36 215 39 218 1.95e-14 76.3
MS.gene30136.t1 AT3G31430 27.473 182 126 4 36 215 134 311 5.48e-13 71.2
MS.gene30136.t1 AT3G47920 27.027 148 104 2 71 216 3 148 1.33e-12 69.7

Find 187 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCCAACTCGAACAATTC+TGG 0.207675 3.3:+42402627 None:intergenic
CGGACTCAGGAGCTAGGTTT+AGG 0.229415 3.3:+42403216 None:intergenic
CAGGGTATAGGTAGTTATTT+AGG 0.284869 3.3:-42402855 MS.gene30136:CDS
GCTTTGTGTAGTCCTGAATT+TGG 0.326028 3.3:-42395256 MS.gene30136:CDS
ATCCTTGACCACTGCTTGAT+TGG 0.334826 3.3:-42403182 MS.gene30136:CDS
GTCAAGGATGGTGTTTGTTT+CGG 0.336720 3.3:+42403196 None:intergenic
AATTGTGTTACGTTCATCAT+AGG 0.337463 3.3:+42402821 None:intergenic
CCTCTTCCTCTTCTTAAATT+CGG 0.338434 3.3:+42402170 None:intergenic
ATTTGCTGCTGATGGGGTTA+CGG 0.347901 3.3:-42402115 MS.gene30136:CDS
CCTTCACACCTTAACATTGC+TGG 0.349351 3.3:-42403266 MS.gene30136:CDS
ACTAGCTGACCGTGAGATTC+AGG 0.350824 3.3:-42403153 MS.gene30136:CDS
ACATAGCAGCAGGATGATTC+TGG 0.351919 3.3:+42402493 None:intergenic
ACCTGAATCATATGGACATC+TGG 0.355218 3.3:-42402926 MS.gene30136:CDS
AATGTAAAATCACCAAATTC+AGG 0.356040 3.3:+42395244 None:intergenic
CCCAGATGTCCATATGATTC+AGG 0.358634 3.3:+42402925 None:intergenic
GTGCTGAAGATATATCTATT+TGG 0.361255 3.3:-42401133 MS.gene30136:CDS
GTATGAAAATGCGTGGCATT+TGG 0.361448 3.3:-42401234 MS.gene30136:CDS
AAGTAAAACCAGGTGGAGTT+AGG 0.369191 3.3:+42395377 None:intergenic
CCTAATGTTGGTCAGGGTAT+AGG 0.371467 3.3:-42402867 MS.gene30136:CDS
AATAGGTCCAAATGTTAGTC+TGG 0.371795 3.3:-42402082 MS.gene30136:CDS
TTTGCTGCTGATGGGGTTAC+GGG 0.373949 3.3:-42402114 MS.gene30136:CDS
TGCAAACAACAAGTGGCTTC+AGG 0.374776 3.3:-42402541 MS.gene30136:CDS
GACATCTGGGTCCAGGTTCA+TGG 0.376818 3.3:-42402912 MS.gene30136:CDS
GCAGGTGGTGTTTCAAGTGC+TGG 0.379151 3.3:-42402447 MS.gene30136:CDS
GGACTCAGGAGCTAGGTTTA+GGG 0.383528 3.3:+42403217 None:intergenic
AACATGGCCAAAGTGAAATA+TGG 0.387005 3.3:-42402351 MS.gene30136:CDS
TTAGGCTGATTGCAACAAAA+AGG 0.391564 3.3:+42402885 None:intergenic
AGCCATACGCTCGCTGAAAT+AGG 0.391985 3.3:+42403127 None:intergenic
CTGATGATGGAATTCGCAAT+TGG 0.397365 3.3:-42402605 MS.gene30136:CDS
CAATCAGCCTAATGTTGGTC+AGG 0.399262 3.3:-42402874 MS.gene30136:CDS
TTTCAGTCCAAGCCGTCCTT+TGG 0.408654 3.3:-42402259 MS.gene30136:CDS
TTGCATTTGATGTTGAAAAC+AGG 0.413286 3.3:-42395129 MS.gene30136:CDS
TGATGATGGAATTCGCAATT+GGG 0.418277 3.3:-42402604 MS.gene30136:CDS
AAATGCGTGGCATTTGGAAC+CGG 0.420457 3.3:-42401228 MS.gene30136:CDS
CGTTCCAACCGCACAAGTAT+TGG 0.420547 3.3:-42398266 MS.gene30136:CDS
CTTTCTTTAGATGATGATAC+AGG 0.424229 3.3:-42400858 MS.gene30136:intron
CTTCCAACTCGAACAATTCT+GGG 0.426689 3.3:+42402628 None:intergenic
ATGGCTCGTGCTTGGAAGCC+AGG 0.426942 3.3:-42403110 MS.gene30136:CDS
GCCAACAGCTGAACACCATT+TGG 0.428004 3.3:-42398343 MS.gene30136:CDS
TTACTTCTAGATACTATGAT+TGG 0.429029 3.3:-42395465 MS.gene30136:CDS
AAATTTAAATATGAGAAACT+TGG 0.439350 3.3:-42402699 MS.gene30136:CDS
GTAGCAGTGGGTTTGAGATC+AGG 0.439478 3.3:+42402579 None:intergenic
GCAGACAACTTCATCATTTG+CGG 0.442580 3.3:-42401064 MS.gene30136:CDS
GCATTTGGAACCGGGGTTCA+AGG 0.448940 3.3:-42401219 MS.gene30136:CDS
ACGATCGAGACACGCTTGCC+TGG 0.453258 3.3:+42403092 None:intergenic
TGTCGATGTGGGTGAAGCTA+TGG 0.454255 3.3:-42398174 MS.gene30136:CDS
TAGTTAAACCAGCAATGTTA+AGG 0.454423 3.3:+42403258 None:intergenic
CTCTGTCGACCTGAATCATA+TGG 0.455323 3.3:-42402934 MS.gene30136:CDS
TGTTACAGGTGTGGCTTGCT+TGG 0.461730 3.3:-42402669 MS.gene30136:CDS
ACAATTATGTTCTGAACAGC+AGG 0.464072 3.3:+42402225 None:intergenic
CTACATCATTTGCTGCTGAT+GGG 0.465968 3.3:-42402122 MS.gene30136:CDS
ATGGTGTTTGTTTCGGACTC+AGG 0.467359 3.3:+42403203 None:intergenic
GTGGCACAACAGATGGGATT+GGG 0.470606 3.3:+42402517 None:intergenic
CCAAGCCGTCCTTTGGTGCT+CGG 0.471203 3.3:-42402252 MS.gene30136:CDS
CAGGTGGTGTTTCAAGTGCT+GGG 0.476453 3.3:-42402446 MS.gene30136:CDS
GCTACATCATTTGCTGCTGA+TGG 0.478154 3.3:-42402123 MS.gene30136:CDS
CGTGGCACAACAGATGGGAT+TGG 0.478185 3.3:+42402516 None:intergenic
TACTCTTCGTCCGAGATTGT+CGG 0.482477 3.3:-42401159 MS.gene30136:CDS
AGGAAATAATAATAGATTCA+AGG 0.483970 3.3:-42395416 MS.gene30136:CDS
TTCTGGCGTGGCACAACAGA+TGG 0.484454 3.3:+42402510 None:intergenic
AGTTCTGATGATGAAGTAGT+TGG 0.485396 3.3:-42395289 MS.gene30136:CDS
CAGACGTCGTTGCCTACAGA+TGG 0.486051 3.3:-42398383 MS.gene30136:CDS
TGCCTATTTCAGCGAGCGTA+TGG 0.489265 3.3:-42403129 MS.gene30136:CDS
TAGTATCTAGAAGTAACATT+TGG 0.492256 3.3:+42395472 None:intergenic
GCAGGGAGGCCGAGCACCAA+AGG 0.492951 3.3:+42402243 None:intergenic
AACAGCACCATATTTCACTT+TGG 0.493300 3.3:+42402344 None:intergenic
AATGCGTGGCATTTGGAACC+GGG 0.494603 3.3:-42401227 MS.gene30136:CDS
TAAGAAGAGGAAGAGGCTGC+TGG 0.495148 3.3:-42402163 MS.gene30136:CDS
CCGAGCACCAAAGGACGGCT+TGG 0.496167 3.3:+42402252 None:intergenic
CCTATACCCTGACCAACATT+AGG 0.498215 3.3:+42402867 None:intergenic
GATGATGAAGTAGTTGGAAA+TGG 0.499058 3.3:-42395283 MS.gene30136:CDS
AGAGACATCTTGTGCGTCCT+CGG 0.499296 3.3:+42402197 None:intergenic
GGCTGTTAACAGCGAGCATA+CGG 0.500217 3.3:+42402412 None:intergenic
GCGAGCGTATGGCTCGTGCT+TGG 0.503834 3.3:-42403118 MS.gene30136:CDS
GCCAAATGGTGTTCAGCTGT+TGG 0.505987 3.3:+42398342 None:intergenic
TGGAAAGTTCGTGTCAGTAC+TGG 0.506120 3.3:-42402738 MS.gene30136:CDS
GAGGACGCACAAGATGTCTC+TGG 0.508029 3.3:-42402195 MS.gene30136:CDS
CACTTGTATCGACCCGGAAG+AGG 0.512615 3.3:+42398314 None:intergenic
TTCGAGTTGGAAGCTGATGA+TGG 0.513177 3.3:-42402618 MS.gene30136:CDS
TTGTTTCGGACTCAGGAGCT+AGG 0.515071 3.3:+42403210 None:intergenic
GTTTCAATTCCATCACAAGA+TGG 0.517536 3.3:-42403045 MS.gene30136:CDS
AAAGCTTGAGAAACATCAAA+AGG 0.520301 3.3:-42395353 MS.gene30136:CDS
CATTTGCGGCTAGTGTCCTC+AGG 0.522206 3.3:-42401050 MS.gene30136:intron
AGACGTCGTTGCCTACAGAT+GGG 0.529635 3.3:-42398382 MS.gene30136:CDS
TTCTGCTTCTACAACTGTCT+CGG 0.530526 3.3:-42402289 MS.gene30136:CDS
CTGCCCGTGTTCTTGAGGAC+AGG 0.530742 3.3:-42403019 MS.gene30136:CDS
GTCGATGTGGGTGAAGCTAT+GGG 0.531304 3.3:-42398173 MS.gene30136:CDS
AAACCCACTGCTACTCGCAT+AGG 0.531501 3.3:-42402570 MS.gene30136:CDS
TCAAGCTTTGAAGTAAAACC+AGG 0.532488 3.3:+42395367 None:intergenic
ATAGGTCCAAATGTTAGTCT+GGG 0.534024 3.3:-42402081 MS.gene30136:CDS
TGATTCAGGTCGACAGAGGT+TGG 0.535620 3.3:+42402939 None:intergenic
ATGCCCAGAATTGTTCGAGT+TGG 0.537003 3.3:-42402631 MS.gene30136:CDS
TCATATGGACATCTGGGTCC+AGG 0.538871 3.3:-42402919 MS.gene30136:CDS
GACACCAATACTTGTGCGGT+TGG 0.542065 3.3:+42398262 None:intergenic
GTGTTCTTGAGGACAGGCCA+TGG 0.545918 3.3:-42403013 MS.gene30136:CDS
TGAAGTAGTTGGAAATGGTA+AGG 0.546016 3.3:-42395278 MS.gene30136:CDS
AAAGGAAGCTTCTGATGAAG+AGG 0.546023 3.3:-42395335 MS.gene30136:CDS
TTACAGATGAGCAAGGCATA+CGG 0.546089 3.3:-42400827 MS.gene30136:CDS
TGTTGCAATCAGCCTAATGT+TGG 0.547767 3.3:-42402879 MS.gene30136:CDS
TACAGATGAGCAAGGCATAC+GGG 0.548378 3.3:-42400826 MS.gene30136:CDS
CCAGCAATGTTAAGGTGTGA+AGG 0.549245 3.3:+42403266 None:intergenic
GCAGAAGGTAAACATTGCAT+TGG 0.549318 3.3:+42402306 None:intergenic
TCAGCATGAAACATAGCAGC+AGG 0.549565 3.3:+42402483 None:intergenic
TCAGCACACGCCGACAATCT+CGG 0.550519 3.3:+42401149 None:intergenic
TGGTTTAACTATTGCTGAAG+AGG 0.550850 3.3:-42403246 MS.gene30136:CDS
AACCCACTGCTACTCGCATA+GGG 0.553263 3.3:-42402569 MS.gene30136:CDS
TTGCCGTGTGTCTGTCGATG+TGG 0.553382 3.3:-42398186 MS.gene30136:CDS
CTTCTAGATACTATGATTGG+TGG 0.553560 3.3:-42395462 MS.gene30136:CDS
GAACAACACCAGGTTGGATA+CGG 0.554055 3.3:+42402961 None:intergenic
GCAAGGGAAACTCACAGCCC+TGG 0.558277 3.3:+42398205 None:intergenic
GTAACAAAATCCTTGAACCC+CGG 0.560543 3.3:+42401209 None:intergenic
GTTAAGGTGTGAAGGATCCA+TGG 0.561224 3.3:+42403274 None:intergenic
TACATCATTTGCTGCTGATG+GGG 0.563180 3.3:-42402121 MS.gene30136:CDS
TGAAAATTGTCATAAAGCCA+TGG 0.564805 3.3:+42402996 None:intergenic
GATGGAATTGAAACAAGAAA+CGG 0.565413 3.3:+42403054 None:intergenic
AGACCCTATGCGAGTAGCAG+TGG 0.565796 3.3:+42402566 None:intergenic
GTGGGACAGTGACATTAATA+CGG 0.566852 3.3:+42402772 None:intergenic
GATGATGGAATTCGCAATTG+GGG 0.566916 3.3:-42402603 MS.gene30136:CDS
TTTGATGGCCTAACTCCACC+TGG 0.569738 3.3:-42395385 MS.gene30136:CDS
TCTCCTTGCTGCTCAGAACA+TGG 0.569836 3.3:-42402367 MS.gene30136:CDS
ACTTGTATCGACCCGGAAGA+GGG 0.570253 3.3:+42398315 None:intergenic
GGTTTAACTATTGCTGAAGA+GGG 0.570705 3.3:-42403245 MS.gene30136:CDS
GCACTTGAAACACCACCTGC+AGG 0.574216 3.3:+42402450 None:intergenic
GTCATGGACCGTATCCAACC+TGG 0.575888 3.3:-42402969 MS.gene30136:CDS
CCTGAATCATATGGACATCT+GGG 0.576120 3.3:-42402925 MS.gene30136:CDS
CCGAATTTAAGAAGAGGAAG+AGG 0.576312 3.3:-42402170 MS.gene30136:CDS
CAAACGAAATCAAGTGAGGA+AGG 0.576657 3.3:-42395436 MS.gene30136:CDS
GTCTCGATCGTCAAAACTGA+TGG 0.576823 3.3:-42403080 MS.gene30136:CDS
GGATGCTGCCCGTGTTCTTG+AGG 0.583116 3.3:-42403024 MS.gene30136:CDS
TGCCGTGTGTCTGTCGATGT+GGG 0.584627 3.3:-42398185 MS.gene30136:CDS
GACGTCGTTGCCTACAGATG+GGG 0.589348 3.3:-42398381 MS.gene30136:CDS
TGATGGAAATGGTGAGAAAG+AGG 0.589570 3.3:-42395179 MS.gene30136:CDS
AACATTGCATTGGAGTGGAA+CGG 0.591185 3.3:+42402316 None:intergenic
CTGAAGATATATCTATTTGG+CGG 0.593323 3.3:-42401130 MS.gene30136:CDS
GTTCAGCTGTTGGCGCGAGG+AGG 0.596192 3.3:+42398352 None:intergenic
ATGGCCTGTCCTCAAGAACA+CGG 0.596786 3.3:+42403015 None:intergenic
CTGGTTCCGAATTTAAGAAG+AGG 0.597278 3.3:-42402176 MS.gene30136:CDS
CTTGTATCGACCCGGAAGAG+GGG 0.597382 3.3:+42398316 None:intergenic
GACCAATCAAGCAGTGGTCA+AGG 0.598354 3.3:+42403180 None:intergenic
CATAAAATTACAGATGAGCA+AGG 0.598679 3.3:-42400834 MS.gene30136:CDS
ACATCGACAGACACACGGCA+AGG 0.600685 3.3:+42398188 None:intergenic
AATCAAGCAGTGGTCAAGGA+TGG 0.601061 3.3:+42403184 None:intergenic
AATGCAATGTCTAAGATCGA+AGG 0.601548 3.3:+42395145 None:intergenic
AGGCTGCTGGAACTTCAGAA+TGG 0.602395 3.3:-42402150 MS.gene30136:CDS
ACAGAGGTTGGAACAACACC+AGG 0.604005 3.3:+42402951 None:intergenic
TCTGGCGTGGCACAACAGAT+GGG 0.604472 3.3:+42402511 None:intergenic
CACCCACATCGACAGACACA+CGG 0.604973 3.3:+42398183 None:intergenic
TTGTATCGACCCGGAAGAGG+GGG 0.605550 3.3:+42398317 None:intergenic
GGAGGCCGAGCACCAAAGGA+CGG 0.606895 3.3:+42402247 None:intergenic
AGGTAAACATTGCATTGGAG+TGG 0.607514 3.3:+42402311 None:intergenic
AAAGTGAAATATGGTGCTGT+TGG 0.608342 3.3:-42402342 MS.gene30136:CDS
GTCTGTATTCGTGATTCTGA+GGG 0.611608 3.3:-42398242 MS.gene30136:CDS
TGTCTGTATTCGTGATTCTG+AGG 0.613267 3.3:-42398243 MS.gene30136:CDS
GAGACAGTTGTAGAAGCAGA+AGG 0.613424 3.3:+42402291 None:intergenic
AATAGGCAGCCTGAATCTCA+CGG 0.617647 3.3:+42403144 None:intergenic
GGTCTGCTGCAAACAACAAG+TGG 0.617765 3.3:-42402548 MS.gene30136:CDS
GGGCAGCATCCATCTTGTGA+TGG 0.618902 3.3:+42403036 None:intergenic
GACCCTATGCGAGTAGCAGT+GGG 0.622890 3.3:+42402567 None:intergenic
ACGGACTTTCAATCTCATGT+AGG 0.624136 3.3:+42402791 None:intergenic
TTGCTGCTGATGGGGTTACG+GGG 0.625549 3.3:-42402113 MS.gene30136:CDS
CATCGACAGACACACGGCAA+GGG 0.628252 3.3:+42398189 None:intergenic
AGGTCCAAATGTTAGTCTGG+GGG 0.633523 3.3:-42402079 MS.gene30136:CDS
GATCGAGAAACTCGAAAGAG+AGG 0.635866 3.3:-42395107 MS.gene30136:CDS
TGGCCTGTCCTCAAGAACAC+GGG 0.636190 3.3:+42403016 None:intergenic
ACGTCGTTGCCTACAGATGG+GGG 0.636650 3.3:-42398380 MS.gene30136:CDS
GTTGGAACGCGAAGAGAAAG+CGG 0.637469 3.3:+42398280 None:intergenic
GGTGTTCAGCTGTTGGCGCG+AGG 0.643395 3.3:+42398349 None:intergenic
AATCAGCCTAATGTTGGTCA+GGG 0.644602 3.3:-42402873 MS.gene30136:CDS
TAGGTCCAAATGTTAGTCTG+GGG 0.645416 3.3:-42402080 MS.gene30136:CDS
ATGCGTGGCATTTGGAACCG+GGG 0.645544 3.3:-42401226 MS.gene30136:CDS
ATGTTGCACTTGTATCGACC+CGG 0.648472 3.3:+42398308 None:intergenic
GGTGAGAAAGAGGAAAGTGT+TGG 0.652185 3.3:-42395169 MS.gene30136:CDS
TGGCCATGTTCTGAGCAGCA+AGG 0.652415 3.3:+42402364 None:intergenic
AGGTTGGAACAACACCAGGT+TGG 0.657973 3.3:+42402955 None:intergenic
TACAGACACCAATACTTGTG+CGG 0.663402 3.3:+42398258 None:intergenic
CATATGATTCAGGTCGACAG+AGG 0.671932 3.3:+42402935 None:intergenic
GGTCCAAATGTTAGTCTGGG+GGG 0.672656 3.3:-42402078 MS.gene30136:intron
GCAGCAGGATGATTCTGGCG+TGG 0.674289 3.3:+42402498 None:intergenic
ATGATGGAATTCGCAATTGG+GGG 0.674973 3.3:-42402602 MS.gene30136:CDS
AGCTTTGAAGTAAAACCAGG+TGG 0.679240 3.3:+42395370 None:intergenic
GCAAATGATGAAGTTGTCTG+CGG 0.686526 3.3:+42401066 None:intergenic
GAACATAATTGTTGACTCCG+AGG 0.692154 3.3:-42402214 MS.gene30136:CDS
CAATTATGTTCTGAACAGCA+GGG 0.697098 3.3:+42402226 None:intergenic
GCAGCAAACGAAATCAAGTG+AGG 0.709271 3.3:-42395440 MS.gene30136:CDS
GTACACGACCAATCAAGCAG+TGG 0.718956 3.3:+42403174 None:intergenic
AGATGAGCAAGGCATACGGG+AGG 0.723229 3.3:-42400823 MS.gene30136:intron
TTATGTTCTGAACAGCAGGG+AGG 0.780148 3.3:+42402229 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.3 gene 42395093 42403294 42395093 ID=MS.gene30136
chr3.3 mRNA 42395093 42403294 42395093 ID=MS.gene30136.t1;Parent=MS.gene30136
chr3.3 exon 42402079 42403294 42402079 ID=MS.gene30136.t1.exon1;Parent=MS.gene30136.t1
chr3.3 CDS 42402079 42403294 42402079 ID=cds.MS.gene30136.t1;Parent=MS.gene30136.t1
chr3.3 exon 42401051 42401275 42401051 ID=MS.gene30136.t1.exon2;Parent=MS.gene30136.t1
chr3.3 CDS 42401051 42401275 42401051 ID=cds.MS.gene30136.t1;Parent=MS.gene30136.t1
chr3.3 exon 42400824 42400870 42400824 ID=MS.gene30136.t1.exon3;Parent=MS.gene30136.t1
chr3.3 CDS 42400824 42400870 42400824 ID=cds.MS.gene30136.t1;Parent=MS.gene30136.t1
chr3.3 exon 42398170 42398405 42398170 ID=MS.gene30136.t1.exon4;Parent=MS.gene30136.t1
chr3.3 CDS 42398170 42398405 42398170 ID=cds.MS.gene30136.t1;Parent=MS.gene30136.t1
chr3.3 exon 42395093 42395498 42395093 ID=MS.gene30136.t1.exon5;Parent=MS.gene30136.t1
chr3.3 CDS 42395093 42395498 42395093 ID=cds.MS.gene30136.t1;Parent=MS.gene30136.t1
Gene Sequence

>MS.gene30136

ATGGATCCTTCACACCTTAACATTGCTGGTTTAACTATTGCTGAAGAGGGCTTAACCCTAAACCTAGCTCCTGAGTCCGAAACAAACACCATCCTTGACCACTGCTTGATTGGTCGTGTACTAGCTGACCGTGAGATTCAGGCTGCCTATTTCAGCGAGCGTATGGCTCGTGCTTGGAAGCCAGGCAAGCGTGTCTCGATCGTCAAAACTGATGGTGACCGTTTCTTGTTTCAATTCCATCACAAGATGGATGCTGCCCGTGTTCTTGAGGACAGGCCATGGCTTTATGACAATTTTCACATCGTCATGGACCGTATCCAACCTGGTGTTGTTCCAACCTCTGTCGACCTGAATCATATGGACATCTGGGTCCAGGTTCATGGCCTTCCTTTTTGTTGCAATCAGCCTAATGTTGGTCAGGGTATAGGTAGTTATTTAGGTGTCCTCAAAACCTATGATGAACGTAACACAATTCATAGTACCTACATGAGATTGAAAGTCCGTATTAATGTCACTGTCCCACTGAAAAAAGAGTGGAAAGTTCGTGTCAGTACTGGTGAATATGTTACTGTTAAATTTAAATATGAGAAACTTGGAGTTTTTTGTTACAGGTGTGGCTTGCTTGGACACACAGATAAAGTATGCCCAGAATTGTTCGAGTTGGAAGCTGATGATGGAATTCGCAATTGGGGGCCTGATCTCAAACCCACTGCTACTCGCATAGGGTCTGCTGCAAACAACAAGTGGCTTCAGGACCCAATCCCATCTGTTGTGCCACGCCAGAATCATCCTGCTGCTATGTTTCATGCTGATCGAAATTTTCCTGCAGGTGGTGTTTCAAGTGCTGGGACTTTTAATGACCGTATGCTCGCTGTTAACAGCCAAATTTCTGCCCTTAAAAATGATCTCCTTGCTGCTCAGAACATGGCCAAAGTGAAATATGGTGCTGTTGGTAACCGTTCCACTCCAATGCAATGTTTACCTTCTGCTTCTACAACTGTCTCGGCATCTGCTTTCAGTCCAAGCCGTCCTTTGGTGCTCGGCCTCCCTGCTGTTCAGAACATAATTGTTGACTCCGAGGACGCACAAGATGTCTCTGGTTCCGAATTTAAGAAGAGGAAGAGGCTGCTGGAACTTCAGAATGGTGAAGCTACATCATTTGCTGCTGATGGGGTTACGGGGAATTTTGTAATAGGTCCAAATGTTAGTCTGGGGGGTGATACAGATATGAATGTACTTTCTAATCCGCTGTTTGTTGAAACTGATGTAATGGCATGCACTGATGATCAGGCCTGCCTAGGTAAATGAGTTTAGTGGCATGGAATTGTCGGGGTTTAGGTAGCCCGAGTGCAATTCCGGACCTTAAGTACCTAGTCCGGCACTTCAATCCGGATCTTTTATTCCTAAGTGAGACTCTTGCTCACCGTAATAAAATTGAAGAACTCCGTTTTTTGCTTGGTTACGATTCTTGTCTCCCTATAGACCGCACCGGTAGAGGCGGGGGTCTAGCTTTATTTTGGCGCAATTCATTCAATTGTCATCTTATTGATTTTTCTAATAATCATGTTACTATGGAGATTGTGGATTCTGTTCATGGTACTTGGAGAATGACAGGTTATTATGGTTACCCCGACGGAGGGCGTAGGCGTGCTGCCTGGAATTTTCTTCGCCAACTCTCTCATCAATTCGCGGGGCCGTGGTGCATTTTTGGCGATTTCAACGAAATCCTTGATGCTAGTGAGAAGAGGGGCCGCAATGATAGACCACAGTGGCTTATTAATGGATTTCGTCAAGCGGTTATTGATTCTGGCTTGTCAGACGTTCCGATAGAAGGCTATCCCTTCACTTGGTTCAAGAGTCTTGGCACGTCGCGAGCGGTGGAGGAAAGGTTAGATCGTGCTCTTGGTAACACCTCTTGGTTTAATCTTTTTCCTAATGCAACTGTTGACACTCTTGTTGCTCCGGCGTCTGATCACTATCCTATTTTATTGAATTGCTCTCCTACACCTCGACCTCAAGTGCATAAGCGCAATTTTCGGTATGAAAATGCGTGGCATTTGGAACCGGGGTTCAAGGATTTTGTTACTAACTCTTGGCAGGTAAATTCTCTGCATACTCTTCGTCCGAGATTGTCGGCGTGTGCTGAAGATATATCTATTTGGCGGAAAACTCACTGTCACCAACTTAAAAAAGACATTGAAGATTGCCGCAGACAACTTCATCATTTGCGGCTAGTGTCCTCAGGTGAGGATCAAGTTCAGATGTTTGATTTGAGGAAGCGAATGCAGCGGTTATTATGTCAGGACGATGCTTATTGGCGGCAACGAGCGAAAATGCATTGGTACAAGGATGGGGACCGAAATACCAAATTTTTTCACGCTTCGGCGACTGCACGTAAGAAGGTAAATCACATTCTTTCTTTAGATGATGATACAGGTCATAAAATTACAGATGAGCAAGGCATACGGGAGGTAGCCAAAAACTATTTTACGAATATTTTTCAAAAGCAAGGTGGCGATTTTTCTTCTGTTATTAATGTCATTAATCCATCTATATCTGGCCTTGATAATGACAAGCTCACGGCCCCCTTTACTATGGCCGAGTTTCGCACAGCTTTATTTGCTATGCAGCCAGACAAATGTTCTGGTCCAGACGGGTACAGCCCGGGATTTTATCAACATTTCTGGAACTTATGCAGTGATGATATTTTTAGAGAATGTTGTGCTTGGTTAGAGACGGGTCATTTCCCCCCTGATTTAAATATAACAAATATTGCTCTTATTCCGAAAGGATCTTCGCAGGCGAGTATGAAAGACTGGCGCCCTATTGCTTTATGTAACGTGTTGTACAAAATTATTTCCAAGGTGTTAGCTAATAGGTTAAAAGAAGTACTGCCTCAATGTATTTCTGATAACCAATCTGCTTTTGTTCTGGGGCGTTCGATATTAGACAATGCGATGGTCGCTATTGAGGTGTTACATTTTATGAAAACGAAGACGCGCGGTAATGACAGGTATGTTGCTTTGAAACTGGACATTAGTAAAGCCTATGACCGAATGGATTGGGACTATCTCCGTGCTGTTTTAGGCAAGATGGGTTTTAATAGTAAATGGATTCATTGGATGAGTATGTGTGTCGATTCTGTAGATTATTCTGTGCTTGTTAACGGTGAACAGGTTGGGCCCATTATTCCAGGTCGCGGTCTCCGTCACGGGGATCCTCTTTCCCCGTATTTATTTATTATCTGTGCTGAAGGTTTGTCTTCTTTGATTAGGGATGCTGAGTCGCGAGGAGTCCTCTCGGGTACAAAAATTTGTCGTAGGGCGCCCCCAGTTTCTCATCTGTTATTTGCTGATGACTGTTTCTTGTTTTTCAAAGCAGATGTTGACGAGGTCACGGTCATGAAGAATATTCTTTCTCTTTATGAAACAGCGTCAGGTCAGGCTATCAGTCTTCCTAAATCAGAAATCTATTGCAGTCGCAATGTTCCTGACCCTCTCAAGCAAACAATTACTACTATCCTTGGTGTTCAGGTAGTACTAGGCACAGGTAAATATTTAGGGCTCCCTTCTATGATAGGTAGGGATCGCAAGGCAACTTTTGCCTATATTAAGGACCGGGTATGGCATAAAATTAATTCATGGAGTGGAAAATGTCTCTCTAAAGCAGGGCGTGAAGTCATGATCAAATCTGTCTTGCAGGCCATTCCTTCTTACATTATGAGTGTTTTTCTTTTACCAACCACTTTGGTCGATTCGATTGAGAAGATGATGAATTCTTTTTGGTGGGGTCATGGACGACCGTCTCAACGGGGTATTCACTGGTTGAGCTGGGAGAAGCTTTCAGTACCAAAGAAATATGGAGGTATGAGTTTCAAAGATCTTACCGCTTTTAATTTGGCTATGTTAGGTAAACAGGGCTGGAAATTTTTAACAGAGCCGGAGTCACTTGTTGCGCGTATTTTCAAAGCACGTTATTACCCCTCGGGGTCTTACCTTACGGCTGTAGTAGGTCATAACCCCAGTTATGTCTGGAGAAGTATTATGCGAGCCAGATTCATTGTTCGTGGTGGAGTTCGGTGGAGGATAGGGTCCGGTGCGGGTATTTCTATTCTTAATGAACCTTGGTTACCTAATGGGGAGTGCATTTCTAGTGAAATCCAAGGAGCTCATTTTGTGCATAACTTCACTATTAATAGTTTAATGCAGATGTACGACAAAAGGTGGAATGAACATGTTGTCAGACAAGTCTTTAGTTCGGATATTGCAGATAAAATCCTTCGTACTCCGCTTCACTCTCAGGTTGAGGACGACAGGCTCATTTGGAAAGCGGAAAGAAACGGTAGGTATTCAGTGCGTAGTGCTTACAGGTTATGTGTATCAGAGTTGGTAGATTCCTCTCATCTTTGGCGTCCGGGGTTTTGGTCAGGCATCTGGAATTTAAAAGTACCACCAAAAGTCAAGAACTTAGTGTGGCGCATGTGTCGGGGCTGTTTGCCGACTCGGGTACGTCTTCTTGACAAGGGTGTTAATTGTCTGACTAATTGTGCTAGTTGTGATTCGAATAATGAAGATCTCAAGCATGTATTTTTTGAGTGTCCGTTTGCTATTCAGGTATGGAACCGGACTGGTTTGTGGGGTAGTATTCAGCATTCTTTATCTCAGAATGACTCGGTGGTGGGTGCTATTTTCTCCCTATTAGAATCTTTATCTACCGAGTTAAGCCAACGTCTCACAGCGGTGTGCTGGAGTATTTGGAAACACCGTAATTTGAGAGTTTGGGATGATGTTACGGAAACAAGCGCAGTGGTTGTGGAGCGGGCTAATCATTTGGTTGTCGATTGGCAGTTGGCGAATTCTCCGACCACTCTCTCGTCCGATACTTCGCAGCAGACGTCGTTGCCTACAGATGGGGGTGCATCCTCCTCGCGCCAACAGCTGAACACCATTTGGCAGCCCCCTCTTCCGGGTCGATACAAGTGCAACATCGATGCCGCTTTCTCTTCGCGTTCCAACCGCACAAGTATTGGTGTCTGTATTCGTGATTCTGAGGGAACTTTTGTGTTGGCCAGGGCTGTGAGTTTCCCTTGCCGTGTGTCTGTCGATGTGGGTGAAGCTATGGGACTGTATGAGGCCTTGCATTGGTTGGGAGACATGCAGATGGACGGTGTTGACTTTGCATTGGATTCTAAATTGACGACTGATGCTTTTCTTTCTTCTGGGAATGACCTATCTGAATTTGGTACTATTATATCTTCTTGTCGTTCTTTCTTTAGTTCTACTTTTGTTAACTCTAGGGTGGATTTTACTAGGAGACAAGCTAACGGTGTTGCCCATTCTCTTGCACGAGAGGCCACGTTACTCGCTAGTCCCGTCACTTATTTTGTTATACCCGATTGTATTGAACCCTTGATCATAAATGAAATGCTATAAGCATGTTTCTTTCAAAAAAAAAAAAAAAATATATATATATATATATAGTAAATCATAAACATAAATTTTACACTAATTAAATATGTTCGATAAAAAATTAGGATTTGATTATATACCTAGTTTATAGGATAAAATTAAAAAAATATAATTATTGACATGCGAACATATTTATAATTTACTATATATATGCAATAAATTATAAACATACATTTTATAGCAATTAAATATGTTCAATAAAAAATTAGAATTTGACTACGTACCTAGTTTATAGGATAAAATCAAAAAATTATAATCATGGACATGTGAACACATTTATAATTTTTATTTATAATGTTAATTTCTGTTTTAAAAATATTTTCTTTATAGAATATATACTAGCTTGACCAAAAAAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGGGTCTTGCTACAAGCCACCCTTTGGTGGTGTCTTTTAGCAAGTCACACCTCAAGGTGTGATTTGCACTTTTTAGTTATAACTTTGCTAAAAGACACCTTCATAAAAGTAAGTTGGTGTCTTTTCGCAAATGCTCATAGAGTAACTTGCTAAAAGACACCTTCACAAAAGGTGTCTTCTAGCAAAACCCTATATATATATATATGCTAGCGAAAATATTACATACTTTAGTGTCTTATGTCATCCCAAGTACACATTTTTTTGAAAAGTGTGTAAAATGGTTGAGAAGTTGTAGAAACTCTCTCTCTCTCTTATTAATGGAAAATACTTGTGTGACATTAATGTCACGGTAACATTAAATCTGAGCTACACATTGGGTGACATTAGATGTGTTGTGTGGCCCAGATGCAATGACAATGACAATGTGTGGTTCAGATTTAATGTCACCGTGACATTAATGTCACCAAAGTGCCACCCGTTATTAATCCATGACTCTTCCCAAGTAAACCCAACCCTTGTCCTTAAGCAAGTCTTTACGTCTCTTTCATAACACAAAAAAACCAATTTTTTTTTTTTTTTGTCATTTTCCTTCACTTTTCCGAAAATGGATGAAAACATCAACATCATAGAAACTAGACAGGAACTCATGGTTTCACCATCTTCTGGTAAAAACCAAACTGTCAAAACTGCATATTTCCTGAAACCATGCAACAAAGAAGACTCTTCTTCTCTTCCACCCCATTTCTTCATTTCCTCTCAAACCACCACAAATTTGCCATTAGAAGTAAAGTACAATGGGTGGAGAACTCCACAAGAAGAATGGAAGAAATGGGTCAAAAAATTAAGACCAAAGTTTGAAATTTTATGGATCAAAACTGGAATATACAATGCAATCATAGCTTCTACTTATGAAGTCAAAAGGGATGATGATTTGATTCTTCAACTTGCTGATAGATGGTGTTCCAAGACCAATACTTTTGTGTTCCCTTGGGGAGAATCAACAATCACATTGGAAGACACAAAGGTTTGTTTTGGTTACTCTCTTTTGGGTGATTCTATTTCTAAACCTCTTGAGAACAGTGAACAAGAAGAAGCTGAAGAAGACTTGATTGAAGCAAGAAGAATGTTTCATAGATCTAAAGCTAAAAAATGTAATCAATTTGCGTGGATGAAGCACTTCTTGGAGAGTGAAAGTAAAGTGGAGCATGAAGCATTTTTGGTTTATTGGTTATCAAGGTTTGTATTTCCTTCAGATTCTTATGATACCATTTCCAAAGCTGTTTTTCCTATTGCGATACTTTTAGCTCATGGAAATAGGATAGCTCTTGGACCTGCTGTTTTAGCTAGTATTTATAGAGACTTAACTTTGCTTAATAGCACCATTAATGAAAATGCCACAACAACCATAAAGAGTTGTAGAGAAACTATTTGGGCACCTTTTCAATTGGTTCAAATTTGGGCTTTAGAGAGATTTCAGGCATTGAAGCCACGTCCTTATGGAAAAGGGCATCACGGTTTACCAAAGATGGCTAGATGGGGTGGAATAGAAATGATGAAAACTAAAATCTTGAAAAAGGTCATGGATTGTGCAGGTTTTGGAGACGGATTTTTGTGGCAACCATATGAGAATTCACCATGCATTGAGGTCTATAATGAAAAGGATATGTGGAGATGTCGTAATCCTTGTTTAGATAAAGAGTTAGAGTCGTTTTCTCGATGTTTAAGGGTTTGTGAGTTGGTTGGTATGGGATGTAAAGAGAAGTACTTCCCTCATCGTGTCGCAATGCAATTTGGTATGGATCAAGATATTCCTGGTGAGGTTTTTCTTGGTAAGAAGGATCCTTGGATGATTTATGACGAGCCAATCCCAATAGATATAGATTTGCTCATTCTATTGTGTTCTAGACAACCAAATGTTACTTCTAGATACTATGATTGGTGGCAGCAAACGAAATCAAGTGAGGAAGGAAATAATAATAGATTCAAGGTTGAAAAATTTGATGGCCTAACTCCACCTGGTTTTACTTCAAAGCTTGAGAAACATCAAAAGGAAGCTTCTGATGAAGAGGATCACGTAGTTTATGAACTATCAAGTTCTGATGATGAAGTAGTTGGAAATGGTAAGGCTTTGTGTAGTCCTGAATTTGGTGATTTTACATTATCAGATGTGCTTGATGAAGATGAAATCAATAGTCTATTTTGTGATGGAAATGGTGAGAAAGAGGAAAGTGTTGGTCCTTCGATCTTAGACATTGCATTTGATGTTGAAAACAGGATCGAGAAACTCGAAAGAGAGGTTGCTAAGCTCTAA
Protein sequence

>MS.gene30136.t1

MDPSHLNIAGLTIAEEGLTLNLAPESETNTILDHCLIGRVLADREIQAAYFSERMARAWKPGKRVSIVKTDGDRFLFQFHHKMDAARVLEDRPWLYDNFHIVMDRIQPGVVPTSVDLNHMDIWVQVHGLPFCCNQPNVGQGIGSYLGVLKTYDERNTIHSTYMRLKVRINVTVPLKKEWKVRVSTGEYVTVKFKYEKLGVFCYRCGLLGHTDKVCPELFELEADDGIRNWGPDLKPTATRIGSAANNKWLQDPIPSVVPRQNHPAAMFHADRNFPAGGVSSAGTFNDRMLAVNSQISALKNDLLAAQNMAKVKYGAVGNRSTPMQCLPSASTTVSASAFSPSRPLVLGLPAVQNIIVDSEDAQDVSGSEFKKRKRLLELQNGEATSFAADGVTGNFVIGPNVSLGVHKRNFRYENAWHLEPGFKDFVTNSWQVNSLHTLRPRLSACAEDISIWRKTHCHQLKKDIEDCRRQLHHLRLVSSDDDTGHKITDEQGIREQTSLPTDGGASSSRQQLNTIWQPPLPGRYKCNIDAAFSSRSNRTSIGVCIRDSEGTFVLARAVSFPCRVSVDVGEAMGLQPNVTSRYYDWWQQTKSSEEGNNNRFKVEKFDGLTPPGFTSKLEKHQKEASDEEDHVVYELSSSDDEVVGNGKALCSPEFGDFTLSDVLDEDEINSLFCDGNGEKEESVGPSILDIAFDVENRIEKLEREVAKL