AlfalfaGEDB | MS.gene050679

M. sativa cultivar XinJiangDaYe / MS.gene050679

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050679.t1	AES70414.1	63.2	315	73	5	1	313	1	274	2.80E-97	365.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050679.t1	O04244	27.2	136	95	2	83	216	6	139	7.7e-11	69.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050679.t1	G7J017	63.2	315	73	5	1	313	1	274	2.0e-97	365.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene050679	MS.gene053747	PPI
MS.gene050679	MS.gene021555	PPI
MS.gene050679	MS.gene44144	PPI
MS.gene96109	MS.gene050679	PPI
MS.gene050679	MS.gene05576	PPI
MS.gene050679	MS.gene060718	PPI
MS.gene050679	MS.gene044729	PPI
MS.gene050679	MS.gene60390	PPI
MS.gene050679	MS.gene48477	PPI
MS.gene050679	MS.gene027169	PPI
MS.gene050679	MS.gene81712	PPI
MS.gene050679	MS.gene019177	PPI
MS.gene050679	MS.gene05436	PPI
MS.gene050679	MS.gene038623	PPI
MS.gene30136	MS.gene050679	PPI
MS.gene050679	MS.gene005120	PPI
MS.gene050679	MS.gene055560	PPI
MS.gene050679	MS.gene024297	PPI
MS.gene050679	MS.gene35202	PPI
MS.gene016102	MS.gene050679	PPI
MS.gene46941	MS.gene050679	PPI
MS.gene96541	MS.gene050679	PPI
MS.gene050679	MS.gene057934	PPI
MS.gene050679	MS.gene37537	PPI
MS.gene050679	MS.gene069786	PPI
MS.gene050679	MS.gene42010	PPI
MS.gene050679	MS.gene025158	PPI
MS.gene46948	MS.gene050679	PPI
MS.gene050679	MS.gene049512	PPI
MS.gene025873	MS.gene050679	PPI
MS.gene050679	MS.gene27329	PPI
MS.gene050679	MS.gene87529	PPI
MS.gene050679	MS.gene41393	PPI
MS.gene050679	MS.gene97410	PPI
MS.gene05358	MS.gene050679	PPI
MS.gene050679	MS.gene010656	PPI
MS.gene93396	MS.gene050679	PPI
MS.gene050679	MS.gene016632	PPI
MS.gene050679	MS.gene018474	PPI
MS.gene050679	MS.gene40469	PPI
MS.gene050679	MS.gene96163	PPI
MS.gene050679	MS.gene65623	PPI
MS.gene050679	MS.gene012748	PPI
MS.gene050679	MS.gene66651	PPI
MS.gene050679	MS.gene02896	PPI
MS.gene050679	MS.gene71588	PPI
MS.gene050679	MS.gene61452	PPI
MS.gene050679	MS.gene05231	PPI
MS.gene050679	MS.gene03149	PPI
MS.gene56991	MS.gene050679	PPI
MS.gene28545	MS.gene050679	PPI
MS.gene050679	MS.gene037049	PPI
MS.gene050679	MS.gene042614	PPI
MS.gene050679	MS.gene026680	PPI
MS.gene050679	MS.gene61499	PPI
MS.gene050679	MS.gene051449	PPI
MS.gene050679	MS.gene042651	PPI
MS.gene050679	MS.gene019165	PPI
MS.gene050679	MS.gene81194	PPI
MS.gene050679	MS.gene060773	PPI
MS.gene050679	MS.gene038394	PPI
MS.gene050679	MS.gene09589	PPI
MS.gene050679	MS.gene019001	PPI
MS.gene050679	MS.gene021965	PPI
MS.gene050679	MS.gene018196	PPI
MS.gene050679	MS.gene53904	PPI
MS.gene050679	MS.gene24070	PPI
MS.gene050679	MS.gene83772	PPI
MS.gene050679	MS.gene73261	PPI
MS.gene050679	MS.gene059736	PPI
MS.gene050679	MS.gene85538	PPI
MS.gene050679	MS.gene025675	PPI
MS.gene050679	MS.gene26844	PPI
MS.gene69490	MS.gene050679	PPI
MS.gene050679	MS.gene055546	PPI
MS.gene015248	MS.gene050679	PPI
MS.gene050679	MS.gene71637	PPI
MS.gene050679	MS.gene55209	PPI
MS.gene050679	MS.gene96174	PPI
MS.gene050679	MS.gene074257	PPI
MS.gene050679	MS.gene04749	PPI
MS.gene050679	MS.gene073573	PPI
MS.gene72520	MS.gene050679	PPI
MS.gene050679	MS.gene28718	PPI
MS.gene013193	MS.gene050679	PPI
MS.gene08106	MS.gene050679	PPI
MS.gene050679	MS.gene63743	PPI
MS.gene050679	MS.gene055223	PPI
MS.gene050679	MS.gene068629	PPI
MS.gene050679	MS.gene004417	PPI
MS.gene050679	MS.gene009781	PPI
MS.gene050679	MS.gene031967	PPI
MS.gene050679	MS.gene21066	PPI
MS.gene050679	MS.gene009213	PPI
MS.gene050679	MS.gene013890	PPI
MS.gene050679	MS.gene98127	PPI
MS.gene050679	MS.gene003264	PPI
MS.gene050679	MS.gene054652	PPI
MS.gene050679	MS.gene000634	PPI
MS.gene050679	MS.gene86849	PPI
MS.gene038676	MS.gene050679	PPI
MS.gene050679	MS.gene80362	PPI
MS.gene050679	MS.gene49057	PPI
MS.gene042651	MS.gene050679	PPI
MS.gene050679	MS.gene89815	PPI
MS.gene050679	MS.gene048088	PPI
MS.gene050679	MS.gene86868	PPI
MS.gene050679	MS.gene058889	PPI
MS.gene050679	MS.gene034643	PPI
MS.gene050679	MS.gene60059	PPI
MS.gene050679	MS.gene012889	PPI
MS.gene050679	MS.gene34463	PPI
MS.gene050679	MS.gene038757	PPI
MS.gene050679	MS.gene000506	PPI
MS.gene20246	MS.gene050679	PPI
MS.gene050679	MS.gene030090	PPI
MS.gene050679	MS.gene51804	PPI
MS.gene050679	MS.gene013644	PPI
MS.gene042559	MS.gene050679	PPI
MS.gene22200	MS.gene050679	PPI
MS.gene050679	MS.gene061579	PPI
MS.gene050679	MS.gene04970	PPI
MS.gene050679	MS.gene26038	PPI
MS.gene34812	MS.gene050679	PPI
MS.gene050679	MS.gene060788	PPI
MS.gene050679	MS.gene43924	PPI
MS.gene050679	MS.gene41643	PPI
MS.gene063214	MS.gene050679	PPI
MS.gene050679	MS.gene022929	PPI
MS.gene050679	MS.gene85295	PPI
MS.gene050679	MS.gene26433	PPI
MS.gene050679	MS.gene007022	PPI
MS.gene067340	MS.gene050679	PPI
MS.gene050679	MS.gene69584	PPI
MS.gene050679	MS.gene52349	PPI
MS.gene050679	MS.gene021647	PPI
MS.gene050679	MS.gene047954	PPI
MS.gene050679	MS.gene30136	PPI
MS.gene050679	MS.gene65471	PPI
MS.gene69495	MS.gene050679	PPI
MS.gene050679	MS.gene071784	PPI
MS.gene050679	MS.gene048342	PPI
MS.gene050679	MS.gene069624	PPI
MS.gene05357	MS.gene050679	PPI
MS.gene050679	MS.gene060386	PPI
MS.gene050679	MS.gene83979	PPI
MS.gene050679	MS.gene073582	PPI
MS.gene050679	MS.gene63762	PPI
MS.gene050679	MS.gene016719	PPI
MS.gene050679	MS.gene048072	PPI
MS.gene050679	MS.gene028714	PPI
MS.gene050679	MS.gene88393	PPI
MS.gene050679	MS.gene042027	PPI
MS.gene050679	MS.gene048706	PPI
MS.gene050679	MS.gene69586	PPI
MS.gene050679	MS.gene45083	PPI
MS.gene050679	MS.gene049561	PPI
MS.gene050679	MS.gene008658	PPI
MS.gene050679	MS.gene47792	PPI
MS.gene021968	MS.gene050679	PPI
MS.gene050679	MS.gene63150	PPI
MS.gene050679	MS.gene03936	PPI
MS.gene050679	MS.gene43393	PPI
MS.gene050679	MS.gene000629	PPI
MS.gene050679	MS.gene072852	PPI
MS.gene050679	MS.gene65474	PPI
MS.gene050679	MS.gene08708	PPI
MS.gene050679	MS.gene04134	PPI
MS.gene050679	MS.gene06164	PPI
MS.gene050679	MS.gene50392	PPI
MS.gene050679	MS.gene86275	PPI
MS.gene050679	MS.gene22730	PPI
MS.gene050679	MS.gene032299	PPI
MS.gene050679	MS.gene017586	PPI
MS.gene050679	MS.gene038098	PPI
MS.gene050679	MS.gene037634	PPI
MS.gene050679	MS.gene057828	PPI
MS.gene050679	MS.gene071320	PPI
MS.gene050679	MS.gene08606	PPI
MS.gene050679	MS.gene012019	PPI
MS.gene050679	MS.gene048747	PPI
MS.gene050679	MS.gene000640	PPI
MS.gene050679	MS.gene061850	PPI
MS.gene050679	MS.gene98756	PPI
MS.gene050679	MS.gene39956	PPI
MS.gene050679	MS.gene028716	PPI
MS.gene050679	MS.gene74639	PPI
MS.gene050679	MS.gene071133	PPI
MS.gene050679	MS.gene64130	PPI
MS.gene050679	MS.gene60266	PPI
MS.gene050679	MS.gene98467	PPI
MS.gene050679	MS.gene024522	PPI
MS.gene42903	MS.gene050679	PPI
MS.gene050679	MS.gene54051	PPI
MS.gene050679	MS.gene22111	PPI
MS.gene050679	MS.gene046089	PPI
MS.gene050679	MS.gene049610	PPI
MS.gene050679	MS.gene010290	PPI
MS.gene050679	MS.gene37955	PPI
MS.gene048747	MS.gene050679	PPI
MS.gene050679	MS.gene92517	PPI
MS.gene050679	MS.gene34197	PPI
MS.gene050679	MS.gene36069	PPI
MS.gene050679	MS.gene045875	PPI
MS.gene050679	MS.gene010890	PPI
MS.gene050679	MS.gene84790	PPI
MS.gene050679	MS.gene71675	PPI
MS.gene050679	MS.gene055683	PPI
MS.gene050679	MS.gene05319	PPI
MS.gene050679	MS.gene039692	PPI
MS.gene050679	MS.gene032311	PPI
MS.gene050679	MS.gene55682	PPI
MS.gene050679	MS.gene002590	PPI
MS.gene050679	MS.gene41979	PPI
MS.gene94654	MS.gene050679	PPI
MS.gene050679	MS.gene008927	PPI
MS.gene050679	MS.gene98616	PPI
MS.gene050679	MS.gene23521	PPI
MS.gene050679	MS.gene77990	PPI
MS.gene050679	MS.gene65546	PPI
MS.gene050679	MS.gene062511	PPI
MS.gene050679	MS.gene008861	PPI
MS.gene32415	MS.gene050679	PPI
MS.gene050679	MS.gene027994	PPI
MS.gene050679	MS.gene059269	PPI
MS.gene035626	MS.gene050679	PPI
MS.gene050679	MS.gene045614	PPI
MS.gene050679	MS.gene024841	PPI
MS.gene050679	MS.gene064966	PPI
MS.gene050679	MS.gene036423	PPI
MS.gene050679	MS.gene76420	PPI
MS.gene30208	MS.gene050679	PPI
MS.gene050679	MS.gene46353	PPI
MS.gene050679	MS.gene40828	PPI
MS.gene050679	MS.gene51799	PPI
MS.gene050679	MS.gene98471	PPI
MS.gene050679	MS.gene062067	PPI
MS.gene050679	MS.gene61651	PPI
MS.gene050679	MS.gene013676	PPI
MS.gene030090	MS.gene050679	PPI
MS.gene95561	MS.gene050679	PPI
MS.gene050679	MS.gene29406	PPI
MS.gene018196	MS.gene050679	PPI
MS.gene065627	MS.gene050679	PPI
MS.gene57019	MS.gene050679	PPI
MS.gene050679	MS.gene09023	PPI
MS.gene050679	MS.gene054508	PPI
MS.gene050679	MS.gene025987	PPI
MS.gene050679	MS.gene95960	PPI
MS.gene050679	MS.gene053373	PPI
MS.gene050679	MS.gene043140	PPI
MS.gene050679	MS.gene041264	PPI
MS.gene050679	MS.gene35175	PPI
MS.gene050679	MS.gene46948	PPI
MS.gene050679	MS.gene44083	PPI
MS.gene050679	MS.gene29321	PPI
MS.gene050679	MS.gene018745	PPI
MS.gene050679	MS.gene025401	PPI
MS.gene050679	MS.gene57044	PPI
MS.gene050679	MS.gene015093	PPI
MS.gene008275	MS.gene050679	PPI
MS.gene050679	MS.gene24278	PPI
MS.gene050679	MS.gene000379	PPI
MS.gene050679	MS.gene34618	PPI
MS.gene050679	MS.gene59460	PPI
MS.gene050679	MS.gene66650	PPI
MS.gene050679	MS.gene009121	PPI
MS.gene050679	MS.gene08713	PPI
MS.gene050679	MS.gene70932	PPI
MS.gene050679	MS.gene09517	PPI
MS.gene050679	MS.gene87845	PPI
MS.gene050679	MS.gene024405	PPI
MS.gene050679	MS.gene32415	PPI
MS.gene050679	MS.gene85308	PPI
MS.gene036423	MS.gene050679	PPI
MS.gene050679	MS.gene62249	PPI
MS.gene050679	MS.gene58419	PPI
MS.gene050679	MS.gene64178	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050679.t1	MTR_3g053920	62.305	321	78	5	1	319	1	280	6.17e-124	358
MS.gene050679.t1	MTR_2g061500	85.271	129	19	0	1	129	1	129	1.49e-78	238
MS.gene050679.t1	MTR_4g103530	53.419	234	54	6	55	286	1	181	9.36e-70	217
MS.gene050679.t1	MTR_7g011710	70.588	85	25	0	55	139	1	85	1.30e-36	130
MS.gene050679.t1	MTR_6g033215	35.673	171	108	1	31	199	124	294	3.67e-36	133
MS.gene050679.t1	MTR_0010s0370	37.989	179	97	6	8	177	4	177	1.94e-33	123
MS.gene050679.t1	MTR_0010s0350	37.989	179	97	6	8	177	4	177	1.94e-33	123
MS.gene050679.t1	MTR_8g463110	67.619	105	34	0	262	366	1	105	1.05e-28	117
MS.gene050679.t1	MTR_1g036720	33.981	103	68	0	11	113	9	111	7.35e-15	70.5
MS.gene050679.t1	MTR_7g089990	36.111	108	65	2	106	209	86	193	4.29e-12	65.5
MS.gene050679.t1	MTR_3g024240	31.667	120	78	2	84	199	55	174	4.21e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050679.t1	AT2G17920	27.570	214	147	5	8	216	9	219	1.07e-19	89.0
MS.gene050679.t1	AT5G36228	26.531	196	138	4	36	228	39	231	1.02e-16	80.9
MS.gene050679.t1	AT3G57587	29.577	142	96	2	77	216	80	219	1.03e-16	80.9
MS.gene050679.t1	AT3G31430	26.291	213	146	4	5	215	108	311	3.36e-16	79.3
MS.gene050679.t1	AT2G41590	28.873	142	97	2	77	216	80	219	4.25e-16	79.3
MS.gene050679.t1	AT2G13450	26.230	183	129	4	36	215	39	218	1.20e-15	78.2
MS.gene050679.t1	AT2G02103	25.822	213	152	3	8	216	9	219	5.27e-15	76.3
MS.gene050679.t1	AT5G25200	25.352	213	153	3	8	216	9	219	6.18e-15	75.9
MS.gene050679.t1	AT5G18636	25.352	213	153	3	8	216	9	219	7.24e-15	75.5
MS.gene050679.t1	AT3G47920	28.169	142	98	2	77	216	9	148	1.84e-14	73.6
MS.gene050679.t1	AT4G02000	27.206	136	95	2	83	216	6	139	1.76e-13	70.9

Find 106 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTGAATCACATTGATTTC+TGG	0.138202	7.3:-598937	MS.gene050679:CDS
TCGAGAACAACAACGGTTTC+TGG	0.223490	7.3:+599208	None:intergenic
CATCGACCTTGTGGTGAAAT+TGG	0.244441	7.3:+599053	None:intergenic
GTTTAGCCTTTGCAGCAATT+TGG	0.253157	7.3:+598372	None:intergenic
AGACATACGCTCACTGAAAT+AGG	0.256666	7.3:+599138	None:intergenic
GATTCCAGCTCAAACAATTC+TGG	0.273451	7.3:+598638	None:intergenic
ACGACTATCATAAGACTTAA+AGG	0.281382	7.3:+598841	None:intergenic
TGGCTCAGATGCGAGGTTTA+TGG	0.281706	7.3:+599228	None:intergenic
GTCTGACGGTGAATTTGTGC+TGG	0.326563	7.3:-598270	MS.gene050679:CDS
TTTGTGCTGGGTTTGGCTGC+TGG	0.328076	7.3:-598257	MS.gene050679:CDS
GTGATTCAGTGGGACAAAAC+TGG	0.330797	7.3:+598949	None:intergenic
GTTCTCGAACATTGCCTAAT+CGG	0.331242	7.3:-599193	MS.gene050679:CDS
TTTAGCCTTTGCAGCAATTT+GGG	0.345418	7.3:+598373	None:intergenic
CGGTGAATTTGTGCTGGGTT+TGG	0.347218	7.3:-598264	MS.gene050679:CDS
ACTGAATCACATTGATTTCT+GGG	0.367380	7.3:-598936	MS.gene050679:CDS
CTCTTACCTTCTTCCTCAAC+AGG	0.368499	7.3:-598314	MS.gene050679:CDS
CGTGGTACTGAAGCAGGAAT+AGG	0.371280	7.3:+598527	None:intergenic
ACCATCGATGTTAAGGTTGT+TGG	0.386606	7.3:+599276	None:intergenic
CAAGTCCACGGTTTACCCTT+TGG	0.387847	7.3:-598911	MS.gene050679:CDS
TACCTTGACCTACTTTCTGT+TGG	0.393464	7.3:+598882	None:intergenic
CAACAAGGACACGACCGATT+AGG	0.395946	7.3:+599179	None:intergenic
AGGTGCTAGTGGTTCTTGTC+AGG	0.398289	7.3:-598342	MS.gene050679:CDS
GTAATTGTAACTTTCTTGCC+TGG	0.398943	7.3:+599103	None:intergenic
TTGTCACAGGTGCGGTCTGT+TGG	0.412415	7.3:-598681	MS.gene050679:CDS
GCTGGTCCGTTTCTTAATGC+TGG	0.423316	7.3:-598239	MS.gene050679:CDS
CCTTGTGGTGAAATTGGAAT+AGG	0.425107	7.3:+599059	None:intergenic
CCATCGATGTTAAGGTTGTT+GGG	0.431832	7.3:+599277	None:intergenic
GTTAAGGTTGTTGGGATCCA+TGG	0.436099	7.3:+599285	None:intergenic
ACAAAACTGGGTACGACACC+AGG	0.437274	7.3:+598962	None:intergenic
AGGTCAGACCATCGATGTTA+AGG	0.439277	7.3:+599269	None:intergenic
CACGCCATTCTTGTTTCAAA+GGG	0.439548	7.3:+598765	None:intergenic
GCAAAGGCTAAACACGGTTC+AGG	0.440363	7.3:-598362	MS.gene050679:CDS
TAGTAACGGTTCCTTTGTTA+CGG	0.443190	7.3:-598735	MS.gene050679:CDS
ATCATTCTGTCGTTGAAACT+TGG	0.449924	7.3:+598443	None:intergenic
ATGTCTCGTGCTTGGAAGCC+AGG	0.453399	7.3:-599121	MS.gene050679:CDS
CAGAAAGTAGGTCAAGGTAT+AGG	0.453798	7.3:-598878	MS.gene050679:CDS
TGTCACAGGTGCGGTCTGTT+GGG	0.454004	7.3:-598680	MS.gene050679:CDS
CTGGTTCCGATTTGAAGAAA+AGG	0.462538	7.3:-598193	MS.gene050679:CDS
CGCTGCCCAAATTGCTGCAA+AGG	0.473524	7.3:-598378	MS.gene050679:CDS
GGTTAAACCCTCCTCTGATA+AGG	0.475559	7.3:+599249	None:intergenic
ATTCCAGCTCAAACAATTCT+GGG	0.477103	7.3:+598639	None:intergenic
TTCTGTTGGATGAAACCAAA+GGG	0.480894	7.3:+598896	None:intergenic
GAATCATCATTTGCTGCTGA+TGG	0.486408	7.3:-598140	MS.gene050679:CDS
TCTGACGGTGAATTTGTGCT+GGG	0.491645	7.3:-598269	MS.gene050679:CDS
CTGTCCCTTTGAAACAAGAA+TGG	0.493273	7.3:-598769	MS.gene050679:CDS
GTGGTACTGAAGCAGGAATA+GGG	0.493715	7.3:+598528	None:intergenic
TTATAGCAGTTAATTGACTC+TGG	0.497479	7.3:+598414	None:intergenic
ACACGCCATTCTTGTTTCAA+AGG	0.498805	7.3:+598764	None:intergenic
CAGAAATCAATGTGATTCAG+TGG	0.501051	7.3:+598938	None:intergenic
AATCATCATTTGCTGCTGAT+GGG	0.504180	7.3:-598139	MS.gene050679:CDS
AAATGCAACAGATGGTGGTC+AGG	0.508197	7.3:-598471	MS.gene050679:CDS
ACTTTCATATTGTCATGGAC+CGG	0.511440	7.3:-598991	MS.gene050679:CDS
ACCGTCAGACAGCAAGTTTG+AGG	0.513455	7.3:+598283	None:intergenic
GGTTTCATCCAACAGAAAGT+AGG	0.516836	7.3:-598890	MS.gene050679:CDS
ATGATTCTTCACCATTCTGA+AGG	0.517798	7.3:+598156	None:intergenic
AAGGATGTTGTTGTTCCTGC+TGG	0.520142	7.3:-598497	MS.gene050679:CDS
GTGCCCAGAATTGTTTGAGC+TGG	0.531217	7.3:-598642	MS.gene050679:CDS
GAAGAAAAGGAAGAGAATGT+TGG	0.533755	7.3:-598180	MS.gene050679:CDS
ACAGCCACGGGCCTTCATCA+AGG	0.536062	7.3:+599020	None:intergenic
GTGAGCGTATGTCTCGTGCT+TGG	0.540385	7.3:-599129	MS.gene050679:CDS
GCCTCAAACTTGCTGTCTGA+CGG	0.540508	7.3:-598284	MS.gene050679:CDS
CAAAGGGACAGTAACATCAA+TGG	0.541078	7.3:+598781	None:intergenic
GATTTCTGGGTGCAAGTCCA+CGG	0.545064	7.3:-598923	MS.gene050679:CDS
TGATAACTTTCATATTGTCA+TGG	0.552037	7.3:-598996	MS.gene050679:CDS
TGGCGTGTCCGTGCTAGTAA+CGG	0.557920	7.3:-598749	MS.gene050679:CDS
GTGTGTCGTGGTACTGAAGC+AGG	0.559588	7.3:+598521	None:intergenic
GTACGACACCAGGGGCAATC+CGG	0.560950	7.3:+598972	None:intergenic
GTCATGGACCGGATTGCCCC+TGG	0.563027	7.3:-598980	MS.gene050679:CDS
GAATTACCTGTTGAGGAAGA+AGG	0.564877	7.3:+598308	None:intergenic
CGGTTTCTGGCTCAGATGCG+AGG	0.565453	7.3:+599221	None:intergenic
TCTGTTGCATTTCGACCAGC+AGG	0.566046	7.3:+598482	None:intergenic
TGATTCAGTGGGACAAAACT+GGG	0.566580	7.3:+598950	None:intergenic
CCTATTCCAATTTCACCACA+AGG	0.568495	7.3:-599059	MS.gene050679:CDS
TCACAACCAGCATTAAGAAA+CGG	0.568808	7.3:+598233	None:intergenic
GAAAGTTATCATACAGCCAC+GGG	0.569417	7.3:+599008	None:intergenic
GCTGCACGCATCCTTGATGA+AGG	0.571959	7.3:-599031	MS.gene050679:CDS
CCCAACAACCTTAACATCGA+TGG	0.575183	7.3:-599277	MS.gene050679:CDS
GTTTCTTAATGCTGGTTGTG+AGG	0.589554	7.3:-598231	MS.gene050679:CDS
TGAGGCTGAATTACCTGTTG+AGG	0.590417	7.3:+598301	None:intergenic
CAAAGGAACCGTTACTAGCA+CGG	0.590633	7.3:+598741	None:intergenic
AGAATGTTGGCCCTTCAGAA+TGG	0.594097	7.3:-598167	MS.gene050679:CDS
GAATACGCATATAAGAGCTA+TGG	0.602257	7.3:+598810	None:intergenic
TGGTCTGACCTTATCAGAGG+AGG	0.603900	7.3:-599257	MS.gene050679:CDS
GGTCTGACCTTATCAGAGGA+GGG	0.610448	7.3:-599256	MS.gene050679:CDS
ATTGAATTTCGCGATCAACA+AGG	0.610626	7.3:+599164	None:intergenic
ATCATCATTTGCTGCTGATG+GGG	0.612968	7.3:-598138	MS.gene050679:CDS
TTCAGTACCACGACACACAA+AGG	0.613295	7.3:-598516	MS.gene050679:CDS
TTTAAAAGTAACCGTAACAA+AGG	0.615879	7.3:+598724	None:intergenic
ATCCAACAGAAAGTAGGTCA+AGG	0.616872	7.3:-598884	MS.gene050679:CDS
TGAAAGTTATCATACAGCCA+CGG	0.617024	7.3:+599007	None:intergenic
TGATTCTTCACCATTCTGAA+GGG	0.621952	7.3:+598157	None:intergenic
ACAACATCCTTTGTGTGTCG+TGG	0.628610	7.3:+598509	None:intergenic
AAACACGGTTCAGGTGCTAG+TGG	0.636490	7.3:-598353	MS.gene050679:CDS
CAAAACTGGGTACGACACCA+GGG	0.636645	7.3:+598963	None:intergenic
GCATCCTTGATGAAGGCCCG+TGG	0.637248	7.3:-599024	MS.gene050679:CDS
CGATGGTCTGACCTTATCAG+AGG	0.640340	7.3:-599260	MS.gene050679:CDS
AGAAATCAATGTGATTCAGT+GGG	0.641223	7.3:+598939	None:intergenic
TTTCTGTTGGATGAAACCAA+AGG	0.643348	7.3:+598895	None:intergenic
TTTGAGCTGGAATCTGATGA+CGG	0.667631	7.3:-598629	MS.gene050679:CDS
GCTGGTCGAAATGCAACAGA+TGG	0.671483	7.3:-598479	MS.gene050679:CDS
GGTCGAAATGCAACAGATGG+TGG	0.674246	7.3:-598476	MS.gene050679:CDS
GCAATGTTCGAGAACAACAA+CGG	0.676603	7.3:+599201	None:intergenic
AAAACTGGGTACGACACCAG+GGG	0.705794	7.3:+598964	None:intergenic
TGCGTGCAGCATCGACCTTG+TGG	0.712710	7.3:+599044	None:intergenic
ATTGCTGCAAAGGCTAAACA+CGG	0.715972	7.3:-598368	MS.gene050679:CDS
TGAAACCAAAGGGTAAACCG+TGG	0.718993	7.3:+598906	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTTTTAAATATGAAAAGCT+GGG	-	chr7.3:598703-598722	MS.gene050679:CDS	20.0%
!!!	TACTTTTAAATATGAAAAGC+TGG	-	chr7.3:598702-598721	MS.gene050679:CDS	20.0%
!	TGATAACTTTCATATTGTCA+TGG	-	chr7.3:598417-598436	MS.gene050679:CDS	25.0%
!	TTTAAAAGTAACCGTAACAA+AGG	+	chr7.3:598692-598711	None:intergenic	25.0%
	ACGACTATCATAAGACTTAA+AGG	+	chr7.3:598575-598594	None:intergenic	30.0%
	AGAAATCAATGTGATTCAGT+GGG	+	chr7.3:598477-598496	None:intergenic	30.0%
!	TCTCTTCCTTTTCTTCAAAT+CGG	+	chr7.3:599229-599248	None:intergenic	30.0%
!	TTATAGCAGTTAATTGACTC+TGG	+	chr7.3:599002-599021	None:intergenic	30.0%
!!	ACTGAATCACATTGATTTCT+GGG	-	chr7.3:598477-598496	MS.gene050679:CDS	30.0%
	AAGCTAGTTACTCAAAGGAT+AGG	-	chr7.3:598832-598851	MS.gene050679:CDS	35.0%
	ACCTTAAGCTAGTTACTCAA+AGG	-	chr7.3:598827-598846	MS.gene050679:CDS	35.0%
	ACTTTCATATTGTCATGGAC+CGG	-	chr7.3:598422-598441	MS.gene050679:CDS	35.0%
	ATCATTCTGTCGTTGAAACT+TGG	+	chr7.3:598973-598992	None:intergenic	35.0%
	ATGATTCTTCACCATTCTGA+AGG	+	chr7.3:599260-599279	None:intergenic	35.0%
	ATTCCAGCTCAAACAATTCT+GGG	+	chr7.3:598777-598796	None:intergenic	35.0%
	ATTGAATTTCGCGATCAACA+AGG	+	chr7.3:598252-598271	None:intergenic	35.0%
	CAGAAATCAATGTGATTCAG+TGG	+	chr7.3:598478-598497	None:intergenic	35.0%
	GAAGAAAAGGAAGAGAATGT+TGG	-	chr7.3:599233-599252	MS.gene050679:CDS	35.0%
	GAATACGCATATAAGAGCTA+TGG	+	chr7.3:598606-598625	None:intergenic	35.0%
	GTAATTGTAACTTTCTTGCC+TGG	+	chr7.3:598313-598332	None:intergenic	35.0%
	TAGTAACGGTTCCTTTGTTA+CGG	-	chr7.3:598678-598697	MS.gene050679:CDS	35.0%
	TCACAACCAGCATTAAGAAA+CGG	+	chr7.3:599183-599202	None:intergenic	35.0%
	TGAAAGTTATCATACAGCCA+CGG	+	chr7.3:598409-598428	None:intergenic	35.0%
	TGATTCTTCACCATTCTGAA+GGG	+	chr7.3:599259-599278	None:intergenic	35.0%
	TTCTGTTGGATGAAACCAAA+GGG	+	chr7.3:598520-598539	None:intergenic	35.0%
	TTTCTGTTGGATGAAACCAA+AGG	+	chr7.3:598521-598540	None:intergenic	35.0%
!	AATCATCATTTGCTGCTGAT+GGG	-	chr7.3:599274-599293	MS.gene050679:CDS	35.0%
!	CAAGGTATAGGTAGCTTTTT+AGG	-	chr7.3:598547-598566	MS.gene050679:intron	35.0%
!	TCCTTTGAGTAACTAGCTTA+AGG	+	chr7.3:598831-598850	None:intergenic	35.0%
!	TTGAGTAACTAGCTTAAGGT+AGG	+	chr7.3:598827-598846	None:intergenic	35.0%
!	TTTAGCCTTTGCAGCAATTT+GGG	+	chr7.3:599043-599062	None:intergenic	35.0%
!!	CACTGAATCACATTGATTTC+TGG	-	chr7.3:598476-598495	MS.gene050679:CDS	35.0%
	ACCATCGATGTTAAGGTTGT+TGG	+	chr7.3:598140-598159	None:intergenic	40.0%
	AGACATACGCTCACTGAAAT+AGG	+	chr7.3:598278-598297	None:intergenic	40.0%
	ATCCAACAGAAAGTAGGTCA+AGG	-	chr7.3:598529-598548	MS.gene050679:CDS	40.0%
	ATTGCTGCAAAGGCTAAACA+CGG	-	chr7.3:599045-599064	MS.gene050679:CDS	40.0%
	CAAAGGGACAGTAACATCAA+TGG	+	chr7.3:598635-598654	None:intergenic	40.0%
	CAGAAAGTAGGTCAAGGTAT+AGG	-	chr7.3:598535-598554	MS.gene050679:intron	40.0%
	CCATCGATGTTAAGGTTGTT+GGG	+	chr7.3:598139-598158	None:intergenic	40.0%
	CCTATTCCAATTTCACCACA+AGG	-	chr7.3:598354-598373	MS.gene050679:CDS	40.0%
	CCTTGTGGTGAAATTGGAAT+AGG	+	chr7.3:598357-598376	None:intergenic	40.0%
	GAAAGTTATCATACAGCCAC+GGG	+	chr7.3:598408-598427	None:intergenic	40.0%
	GAATTACCTGTTGAGGAAGA+AGG	+	chr7.3:599108-599127	None:intergenic	40.0%
	GATTCCAGCTCAAACAATTC+TGG	+	chr7.3:598778-598797	None:intergenic	40.0%
	GCAATGTTCGAGAACAACAA+CGG	+	chr7.3:598215-598234	None:intergenic	40.0%
	GGTTTCATCCAACAGAAAGT+AGG	-	chr7.3:598523-598542	MS.gene050679:CDS	40.0%
	GTTCTCGAACATTGCCTAAT+CGG	-	chr7.3:598220-598239	MS.gene050679:CDS	40.0%
	TACCTTGACCTACTTTCTGT+TGG	+	chr7.3:598534-598553	None:intergenic	40.0%
	TGATTCAGTGGGACAAAACT+GGG	+	chr7.3:598466-598485	None:intergenic	40.0%
	TTTGAGCTGGAATCTGATGA+CGG	-	chr7.3:598784-598803	MS.gene050679:CDS	40.0%
!	ACACGCCATTCTTGTTTCAA+AGG	+	chr7.3:598652-598671	None:intergenic	40.0%
!	ATCATCATTTGCTGCTGATG+GGG	-	chr7.3:599275-599294	MS.gene050679:CDS	40.0%
!	CACGCCATTCTTGTTTCAAA+GGG	+	chr7.3:598651-598670	None:intergenic	40.0%
!	CTGGTTCCGATTTGAAGAAA+AGG	-	chr7.3:599220-599239	MS.gene050679:CDS	40.0%
!	GAATCATCATTTGCTGCTGA+TGG	-	chr7.3:599273-599292	MS.gene050679:CDS	40.0%
!	GAGGAGTCTCATGATTTTAC+TGG	-	chr7.3:599201-599220	MS.gene050679:CDS	40.0%
!	GTTTAGCCTTTGCAGCAATT+TGG	+	chr7.3:599044-599063	None:intergenic	40.0%
!	GTTTCTTAATGCTGGTTGTG+AGG	-	chr7.3:599182-599201	MS.gene050679:CDS	40.0%
!!	CTGTCCCTTTGAAACAAGAA+TGG	-	chr7.3:598644-598663	MS.gene050679:CDS	40.0%
!!	TGATGACGGTGTTCGTAATT+GGG	-	chr7.3:598798-598817	MS.gene050679:CDS	40.0%
	ACAACATCCTTTGTGTGTCG+TGG	+	chr7.3:598907-598926	None:intergenic	45.0%
	AGAATGTTGGCCCTTCAGAA+TGG	-	chr7.3:599246-599265	MS.gene050679:CDS	45.0%
	AGGTCAGACCATCGATGTTA+AGG	+	chr7.3:598147-598166	None:intergenic	45.0%
	CAAAGGAACCGTTACTAGCA+CGG	+	chr7.3:598675-598694	None:intergenic	45.0%
	CATCGACCTTGTGGTGAAAT+TGG	+	chr7.3:598363-598382	None:intergenic	45.0%
	CCCAACAACCTTAACATCGA+TGG	-	chr7.3:598136-598155	MS.gene050679:CDS	45.0%
	CTCTTACCTTCTTCCTCAAC+AGG	-	chr7.3:599099-599118	MS.gene050679:CDS	45.0%
	GGTTAAACCCTCCTCTGATA+AGG	+	chr7.3:598167-598186	None:intergenic	45.0%
	GTGATTCAGTGGGACAAAAC+TGG	+	chr7.3:598467-598486	None:intergenic	45.0%
	TCGAGAACAACAACGGTTTC+TGG	+	chr7.3:598208-598227	None:intergenic	45.0%
	TCTGACGGTGAATTTGTGCT+GGG	-	chr7.3:599144-599163	MS.gene050679:CDS	45.0%
	TGAAACCAAAGGGTAAACCG+TGG	+	chr7.3:598510-598529	None:intergenic	45.0%
	TGAGGCTGAATTACCTGTTG+AGG	+	chr7.3:599115-599134	None:intergenic	45.0%
	TTCAGTACCACGACACACAA+AGG	-	chr7.3:598897-598916	MS.gene050679:CDS	45.0%
!	AAATGCAACAGATGGTGGTC+AGG	-	chr7.3:598942-598961	MS.gene050679:CDS	45.0%
!	AAGGATGTTGTTGTTCCTGC+TGG	-	chr7.3:598916-598935	MS.gene050679:CDS	45.0%
!	GATGACGGTGTTCGTAATTG+GGG	-	chr7.3:598799-598818	MS.gene050679:CDS	45.0%
!	GTGGTACTGAAGCAGGAATA+GGG	+	chr7.3:598888-598907	None:intergenic	45.0%
!!	CTGATGACGGTGTTCGTAAT+TGG	-	chr7.3:598797-598816	MS.gene050679:CDS	45.0%
!!!	AGCTGGGTGTTTTTTGTCAC+AGG	-	chr7.3:598719-598738	MS.gene050679:CDS	45.0%
!!!	GGTGTTTTTTGTCACAGGTG+CGG	-	chr7.3:598724-598743	MS.gene050679:CDS	45.0%
	AAAACTGGGTACGACACCAG+GGG	+	chr7.3:598452-598471	None:intergenic	50.0%
	AAACACGGTTCAGGTGCTAG+TGG	-	chr7.3:599060-599079	MS.gene050679:CDS	50.0%
	ACAAAACTGGGTACGACACC+AGG	+	chr7.3:598454-598473	None:intergenic	50.0%
	ACCGTCAGACAGCAAGTTTG+AGG	+	chr7.3:599133-599152	None:intergenic	50.0%
	AGGTGCTAGTGGTTCTTGTC+AGG	-	chr7.3:599071-599090	MS.gene050679:CDS	50.0%
	CAAAACTGGGTACGACACCA+GGG	+	chr7.3:598453-598472	None:intergenic	50.0%
	CAACAAGGACACGACCGATT+AGG	+	chr7.3:598237-598256	None:intergenic	50.0%
	CAAGTCCACGGTTTACCCTT+TGG	-	chr7.3:598502-598521	MS.gene050679:CDS	50.0%
	CGATGGTCTGACCTTATCAG+AGG	-	chr7.3:598153-598172	MS.gene050679:CDS	50.0%
	CGGTGAATTTGTGCTGGGTT+TGG	-	chr7.3:599149-599168	MS.gene050679:CDS	50.0%
	GCAAAGGCTAAACACGGTTC+AGG	-	chr7.3:599051-599070	MS.gene050679:CDS	50.0%
	GCTGGTCCGTTTCTTAATGC+TGG	-	chr7.3:599174-599193	MS.gene050679:CDS	50.0%
	GCTGGTCGAAATGCAACAGA+TGG	-	chr7.3:598934-598953	MS.gene050679:CDS	50.0%
	GGTCGAAATGCAACAGATGG+TGG	-	chr7.3:598937-598956	MS.gene050679:CDS	50.0%
	GGTCTGACCTTATCAGAGGA+GGG	-	chr7.3:598157-598176	MS.gene050679:CDS	50.0%
	GTCTGACGGTGAATTTGTGC+TGG	-	chr7.3:599143-599162	MS.gene050679:CDS	50.0%
	GTGCCCAGAATTGTTTGAGC+TGG	-	chr7.3:598771-598790	MS.gene050679:CDS	50.0%
	GTTCTGCTGCAACCAACTGA+TGG	-	chr7.3:598854-598873	MS.gene050679:CDS	50.0%
	TCTGTTGCATTTCGACCAGC+AGG	+	chr7.3:598934-598953	None:intergenic	50.0%
	TGCAACCAACTGATGGCTAC+AGG	-	chr7.3:598861-598880	MS.gene050679:CDS	50.0%
	TGGCTCAGATGCGAGGTTTA+TGG	+	chr7.3:598188-598207	None:intergenic	50.0%
	TGGTCTGACCTTATCAGAGG+AGG	-	chr7.3:598156-598175	MS.gene050679:CDS	50.0%
!	CGTGGTACTGAAGCAGGAAT+AGG	+	chr7.3:598889-598908	None:intergenic	50.0%
!	GATTTCTGGGTGCAAGTCCA+CGG	-	chr7.3:598490-598509	MS.gene050679:CDS	50.0%
!!	GCCTCAAACTTGCTGTCTGA+CGG	-	chr7.3:599129-599148	MS.gene050679:CDS	50.0%
	AGGGTCCTGTAGCCATCAGT+TGG	+	chr7.3:598869-598888	None:intergenic	55.0%
	ATGTCTCGTGCTTGGAAGCC+AGG	-	chr7.3:598292-598311	MS.gene050679:CDS	55.0%
	CGCTGCCCAAATTGCTGCAA+AGG	-	chr7.3:599035-599054	MS.gene050679:CDS	55.0%
	GCTGCACGCATCCTTGATGA+AGG	-	chr7.3:598382-598401	MS.gene050679:CDS	55.0%
	GTGAGCGTATGTCTCGTGCT+TGG	-	chr7.3:598284-598303	MS.gene050679:CDS	55.0%
	TGGCGTGTCCGTGCTAGTAA+CGG	-	chr7.3:598664-598683	MS.gene050679:CDS	55.0%
	TGTCACAGGTGCGGTCTGTT+GGG	-	chr7.3:598733-598752	MS.gene050679:CDS	55.0%
	TTGTCACAGGTGCGGTCTGT+TGG	-	chr7.3:598732-598751	MS.gene050679:CDS	55.0%
	TTTGTGCTGGGTTTGGCTGC+TGG	-	chr7.3:599156-599175	MS.gene050679:CDS	55.0%
!	GTGTGTCGTGGTACTGAAGC+AGG	+	chr7.3:598895-598914	None:intergenic	55.0%
	ACAGCCACGGGCCTTCATCA+AGG	+	chr7.3:598396-598415	None:intergenic	60.0%
	GCATCCTTGATGAAGGCCCG+TGG	-	chr7.3:598389-598408	MS.gene050679:CDS	60.0%
	GTACGACACCAGGGGCAATC+CGG	+	chr7.3:598444-598463	None:intergenic	60.0%
!!	CGGTTTCTGGCTCAGATGCG+AGG	+	chr7.3:598195-598214	None:intergenic	60.0%
!!	TGCGTGCAGCATCGACCTTG+TGG	+	chr7.3:598372-598391	None:intergenic	60.0%
	GTCATGGACCGGATTGCCCC+TGG	-	chr7.3:598433-598452	MS.gene050679:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	598130	599305	598130	ID=MS.gene050679
chr7.3	mRNA	598130	599305	598130	ID=MS.gene050679.t1;Parent=MS.gene050679
chr7.3	exon	598628	599305	598628	ID=MS.gene050679.t1.exon1;Parent=MS.gene050679.t1
chr7.3	CDS	598628	599305	598628	ID=cds.MS.gene050679.t1;Parent=MS.gene050679.t1
chr7.3	exon	598130	598552	598130	ID=MS.gene050679.t1.exon2;Parent=MS.gene050679.t1
chr7.3	CDS	598130	598552	598130	ID=cds.MS.gene050679.t1;Parent=MS.gene050679.t1

Gene Sequence

>MS.gene050679

ATGGATCCCAACAACCTTAACATCGATGGTCTGACCTTATCAGAGGAGGGTTTAACCATAAACCTCGCATCTGAGCCAGAAACCGTTGTTGTTCTCGAACATTGCCTAATCGGTCGTGTCCTTGTTGATCGCGAAATTCAATTCGCCTATTTCAGTGAGCGTATGTCTCGTGCTTGGAAGCCAGGCAAGAAAGTTACAATTACTAAGTGTGTGTCTGATCGTTACCTATTCCAATTTCACCACAAGGTCGATGCTGCACGCATCCTTGATGAAGGCCCGTGGCTGTATGATAACTTTCATATTGTCATGGACCGGATTGCCCCTGGTGTCGTACCCAGTTTTGTCCCACTGAATCACATTGATTTCTGGGTGCAAGTCCACGGTTTACCCTTTGGTTTCATCCAACAGAAAGTAGGTCAAGGTATAGGTAGCTTTTTAGGTACCTTTAAGTCTTATGATAGTCGTAACTCAATCCATAGCTCTTATATGCGTATTCGTGTTGCCATTGATGTTACTGTCCCTTTGAAACAAGAATGGCGTGTCCGTGCTAGTAACGGTTCCTTTGTTACGGTTACTTTTAAATATGAAAAGCTGGGTGTTTTTTGTCACAGGTGCGGTCTGTTGGGACATACTGACAAAGTGTGCCCAGAATTGTTTGAGCTGGAATCTGATGACGGTGTTCGTAATTGGGGCGCCTACCTTAAGCTAGTTACTCAAAGGATAGGTTCTGCTGCAACCAACTGATGGCTACAGGACCCTATTCCTGCTTCAGTACCACGACACACAAAGGATGTTGTTGTTCCTGCTGGTCGAAATGCAACAGATGGTGGTCAGGTTGTACCAAGTTTCAACGACAGAATGATTGCTGTCCAGAGTCAATTAACTGCTATAAAACATGATGTTCTCGCTGCCCAAATTGCTGCAAAGGCTAAACACGGTTCAGGTGCTAGTGGTTCTTGTCAGGTGCATCTCTTACCTTCTTCCTCAACAGGTAATTCAGCCTCAAACTTGCTGTCTGACGGTGAATTTGTGCTGGGTTTGGCTGCTGGTCCGTTTCTTAATGCTGGTTGTGAGGAGTCTCATGATTTTACTGGTTCCGATTTGAAGAAAAGGAAGAGAATGTTGGCCCTTCAGAATGGTGAAGAATCATCATTTGCTGCTGATGGGGATGAGTGA

Protein sequence

>MS.gene050679.t1

MDPNNLNIDGLTLSEEGLTINLASEPETVVVLEHCLIGRVLVDREIQFAYFSERMSRAWKPGKKVTITKCVSDRYLFQFHHKVDAARILDEGPWLYDNFHIVMDRIAPGVVPSFVPLNHIDFWVQVHGLPFGFIQQKVGQGIGSFLGTFKSYDSRNSIHSSYMRIRVAIDVTVPLKQEWRVRASNGSFVTVTFKYEKLGVFCHRCGLLGHTDKVCPELFELESDDGDPIPASVPRHTKDVVVPAGRNATDGGQVVPSFNDRMIAVQSQLTAIKHDVLAAQIAAKAKHGSGASGSCQVHLLPSSSTGNSASNLLSDGEFVLGLAAGPFLNAGCEESHDFTGSDLKKRKRMLALQNGEESSFAADGDE