AlfalfaGEDB | MS.gene048342

M. sativa cultivar XinJiangDaYe / MS.gene048342

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048342.t1	PNX72859.1	44.9	376	186	7	5	375	124	483	1.10E-81	313.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048342.t1	A0A392MN10	55.2	248	110	1	5	252	25	271	1.3e-82	316.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene55209	MS.gene048342	PPI
MS.gene64178	MS.gene048342	PPI
MS.gene030090	MS.gene048342	PPI
MS.gene95561	MS.gene048342	PPI
MS.gene96174	MS.gene048342	PPI
MS.gene018196	MS.gene048342	PPI
MS.gene048342	MS.gene60390	PPI
MS.gene065627	MS.gene048342	PPI
MS.gene57019	MS.gene048342	PPI
MS.gene018745	MS.gene048342	PPI
MS.gene65546	MS.gene048342	PPI
MS.gene032299	MS.gene048342	PPI
MS.gene03936	MS.gene048342	PPI
MS.gene021968	MS.gene048342	PPI
MS.gene60059	MS.gene048342	PPI
MS.gene062511	MS.gene048342	PPI
MS.gene43393	MS.gene048342	PPI
MS.gene34197	MS.gene048342	PPI
MS.gene055560	MS.gene048342	PPI
MS.gene008275	MS.gene048342	PPI
MS.gene34618	MS.gene048342	PPI
MS.gene048342	MS.gene025158	PPI
MS.gene59460	MS.gene048342	PPI
MS.gene038757	MS.gene048342	PPI
MS.gene064966	MS.gene048342	PPI
MS.gene016632	MS.gene048342	PPI
MS.gene048072	MS.gene048342	PPI
MS.gene002590	MS.gene048342	PPI
MS.gene025987	MS.gene048342	PPI
MS.gene05436	MS.gene048342	PPI
MS.gene000640	MS.gene048342	PPI
MS.gene43924	MS.gene048342	PPI
MS.gene047954	MS.gene048342	PPI
MS.gene35175	MS.gene048342	PPI
MS.gene036423	MS.gene048342	PPI
MS.gene048342	MS.gene043140	PPI
MS.gene069786	MS.gene048342	PPI
MS.gene054652	MS.gene048342	PPI
MS.gene048342	MS.gene03149	PPI
MS.gene013890	MS.gene048342	PPI
MS.gene060773	MS.gene048342	PPI
MS.gene61651	MS.gene048342	PPI
MS.gene66651	MS.gene048342	PPI
MS.gene96109	MS.gene048342	PPI
MS.gene35202	MS.gene048342	PPI
MS.gene051449	MS.gene048342	PPI
MS.gene015093	MS.gene048342	PPI
MS.gene007022	MS.gene048342	PPI
MS.gene048342	MS.gene24278	PPI
MS.gene048342	MS.gene59460	PPI
MS.gene024841	MS.gene048342	PPI
MS.gene48477	MS.gene048342	PPI
MS.gene51804	MS.gene048342	PPI
MS.gene045875	MS.gene048342	PPI
MS.gene36069	MS.gene048342	PPI
MS.gene038394	MS.gene048342	PPI
MS.gene30136	MS.gene048342	PPI
MS.gene95960	MS.gene048342	PPI
MS.gene008861	MS.gene048342	PPI
MS.gene61452	MS.gene048342	PPI
MS.gene73261	MS.gene048342	PPI
MS.gene024297	MS.gene048342	PPI
MS.gene016102	MS.gene048342	PPI
MS.gene46941	MS.gene048342	PPI
MS.gene96541	MS.gene048342	PPI
MS.gene049561	MS.gene048342	PPI
MS.gene45083	MS.gene048342	PPI
MS.gene000634	MS.gene048342	PPI
MS.gene009781	MS.gene048342	PPI
MS.gene85295	MS.gene048342	PPI
MS.gene47792	MS.gene048342	PPI
MS.gene46948	MS.gene048342	PPI
MS.gene025873	MS.gene048342	PPI
MS.gene012019	MS.gene048342	PPI
MS.gene06164	MS.gene048342	PPI
MS.gene025401	MS.gene048342	PPI
MS.gene05358	MS.gene048342	PPI
MS.gene048342	MS.gene44144	PPI
MS.gene074257	MS.gene048342	PPI
MS.gene010890	MS.gene048342	PPI
MS.gene000629	MS.gene048342	PPI
MS.gene09023	MS.gene048342	PPI
MS.gene059269	MS.gene048342	PPI
MS.gene041264	MS.gene048342	PPI
MS.gene41979	MS.gene048342	PPI
MS.gene000506	MS.gene048342	PPI
MS.gene74639	MS.gene048342	PPI
MS.gene024522	MS.gene048342	PPI
MS.gene05319	MS.gene048342	PPI
MS.gene88393	MS.gene048342	PPI
MS.gene038098	MS.gene048342	PPI
MS.gene56991	MS.gene048342	PPI
MS.gene048342	MS.gene21066	PPI
MS.gene85538	MS.gene048342	PPI
MS.gene22111	MS.gene048342	PPI
MS.gene055223	MS.gene048342	PPI
MS.gene28545	MS.gene048342	PPI
MS.gene28718	MS.gene048342	PPI
MS.gene053747	MS.gene048342	PPI
MS.gene29321	MS.gene048342	PPI
MS.gene061850	MS.gene048342	PPI
MS.gene069624	MS.gene048342	PPI
MS.gene048088	MS.gene048342	PPI
MS.gene008658	MS.gene048342	PPI
MS.gene018474	MS.gene048342	PPI
MS.gene048342	MS.gene018474	PPI
MS.gene048342	MS.gene86868	PPI
MS.gene003264	MS.gene048342	PPI
MS.gene073582	MS.gene048342	PPI
MS.gene058889	MS.gene048342	PPI
MS.gene98616	MS.gene048342	PPI
MS.gene40828	MS.gene048342	PPI
MS.gene046089	MS.gene048342	PPI
MS.gene77990	MS.gene048342	PPI
MS.gene09517	MS.gene048342	PPI
MS.gene69586	MS.gene048342	PPI
MS.gene042027	MS.gene048342	PPI
MS.gene69490	MS.gene048342	PPI
MS.gene043140	MS.gene048342	PPI
MS.gene013644	MS.gene048342	PPI
MS.gene84790	MS.gene048342	PPI
MS.gene80362	MS.gene048342	PPI
MS.gene72520	MS.gene048342	PPI
MS.gene37537	MS.gene048342	PPI
MS.gene013193	MS.gene048342	PPI
MS.gene08106	MS.gene048342	PPI
MS.gene24278	MS.gene048342	PPI
MS.gene025158	MS.gene048342	PPI
MS.gene027994	MS.gene048342	PPI
MS.gene61499	MS.gene048342	PPI
MS.gene068629	MS.gene048342	PPI
MS.gene048342	MS.gene61499	PPI
MS.gene019001	MS.gene048342	PPI
MS.gene63743	MS.gene048342	PPI
MS.gene63150	MS.gene048342	PPI
MS.gene54051	MS.gene048342	PPI
MS.gene98127	MS.gene048342	PPI
MS.gene03149	MS.gene048342	PPI
MS.gene024405	MS.gene048342	PPI
MS.gene012748	MS.gene048342	PPI
MS.gene04134	MS.gene048342	PPI
MS.gene83979	MS.gene048342	PPI
MS.gene062067	MS.gene048342	PPI
MS.gene92517	MS.gene048342	PPI
MS.gene057934	MS.gene048342	PPI
MS.gene57044	MS.gene048342	PPI
MS.gene054508	MS.gene048342	PPI
MS.gene60390	MS.gene048342	PPI
MS.gene89815	MS.gene048342	PPI
MS.gene98471	MS.gene048342	PPI
MS.gene028714	MS.gene048342	PPI
MS.gene049610	MS.gene048342	PPI
MS.gene038676	MS.gene048342	PPI
MS.gene060718	MS.gene048342	PPI
MS.gene71637	MS.gene048342	PPI
MS.gene042651	MS.gene048342	PPI
MS.gene93396	MS.gene048342	PPI
MS.gene05576	MS.gene048342	PPI
MS.gene048342	MS.gene022929	PPI
MS.gene048342	MS.gene007022	PPI
MS.gene52349	MS.gene048342	PPI
MS.gene05231	MS.gene048342	PPI
MS.gene98467	MS.gene048342	PPI
MS.gene46353	MS.gene048342	PPI
MS.gene010656	MS.gene048342	PPI
MS.gene42010	MS.gene048342	PPI
MS.gene037049	MS.gene048342	PPI
MS.gene034643	MS.gene048342	PPI
MS.gene40469	MS.gene048342	PPI
MS.gene037634	MS.gene048342	PPI
MS.gene000379	MS.gene048342	PPI
MS.gene64130	MS.gene048342	PPI
MS.gene050679	MS.gene048342	PPI
MS.gene24070	MS.gene048342	PPI
MS.gene60266	MS.gene048342	PPI
MS.gene20246	MS.gene048342	PPI
MS.gene016719	MS.gene048342	PPI
MS.gene010290	MS.gene048342	PPI
MS.gene071784	MS.gene048342	PPI
MS.gene048342	MS.gene88393	PPI
MS.gene061579	MS.gene048342	PPI
MS.gene039692	MS.gene048342	PPI
MS.gene76420	MS.gene048342	PPI
MS.gene071320	MS.gene048342	PPI
MS.gene65471	MS.gene048342	PPI
MS.gene048342	MS.gene008658	PPI
MS.gene026680	MS.gene048342	PPI
MS.gene26433	MS.gene048342	PPI
MS.gene34463	MS.gene048342	PPI
MS.gene22730	MS.gene048342	PPI
MS.gene042559	MS.gene048342	PPI
MS.gene22200	MS.gene048342	PPI
MS.gene071133	MS.gene048342	PPI
MS.gene53904	MS.gene048342	PPI
MS.gene26038	MS.gene048342	PPI
MS.gene08606	MS.gene048342	PPI
MS.gene08708	MS.gene048342	PPI
MS.gene85308	MS.gene048342	PPI
MS.gene49057	MS.gene048342	PPI
MS.gene015248	MS.gene048342	PPI
MS.gene012889	MS.gene048342	PPI
MS.gene053373	MS.gene048342	PPI
MS.gene34812	MS.gene048342	PPI
MS.gene02896	MS.gene048342	PPI
MS.gene048342	MS.gene000640	PPI
MS.gene44083	MS.gene048342	PPI
MS.gene55682	MS.gene048342	PPI
MS.gene04970	MS.gene048342	PPI
MS.gene51799	MS.gene048342	PPI
MS.gene58419	MS.gene048342	PPI
MS.gene067340	MS.gene048342	PPI
MS.gene048342	MS.gene74639	PPI
MS.gene048342	MS.gene071133	PPI
MS.gene96163	MS.gene048342	PPI
MS.gene65623	MS.gene048342	PPI
MS.gene08713	MS.gene048342	PPI
MS.gene65474	MS.gene048342	PPI
MS.gene23521	MS.gene048342	PPI
MS.gene019177	MS.gene048342	PPI
MS.gene049512	MS.gene048342	PPI
MS.gene29406	MS.gene048342	PPI
MS.gene048342	MS.gene37955	PPI
MS.gene038623	MS.gene048342	PPI
MS.gene86275	MS.gene048342	PPI
MS.gene05357	MS.gene048342	PPI
MS.gene032311	MS.gene048342	PPI
MS.gene87529	MS.gene048342	PPI
MS.gene048342	MS.gene84790	PPI
MS.gene004417	MS.gene048342	PPI
MS.gene86849	MS.gene048342	PPI
MS.gene50392	MS.gene048342	PPI
MS.gene37955	MS.gene048342	PPI
MS.gene072852	MS.gene048342	PPI
MS.gene41393	MS.gene048342	PPI
MS.gene39956	MS.gene048342	PPI
MS.gene017586	MS.gene048342	PPI
MS.gene98756	MS.gene048342	PPI
MS.gene09589	MS.gene048342	PPI
MS.gene048342	MS.gene027994	PPI
MS.gene44144	MS.gene048342	PPI
MS.gene057828	MS.gene048342	PPI
MS.gene044729	MS.gene048342	PPI
MS.gene66650	MS.gene048342	PPI
MS.gene005120	MS.gene048342	PPI
MS.gene86868	MS.gene048342	PPI
MS.gene060386	MS.gene048342	PPI
MS.gene71588	MS.gene048342	PPI
MS.gene048342	MS.gene61651	PPI
MS.gene04749	MS.gene048342	PPI
MS.gene059736	MS.gene048342	PPI
MS.gene70932	MS.gene048342	PPI
MS.gene027169	MS.gene048342	PPI
MS.gene048342	MS.gene025987	PPI
MS.gene62249	MS.gene048342	PPI
MS.gene81194	MS.gene048342	PPI
MS.gene022929	MS.gene048342	PPI
MS.gene025675	MS.gene048342	PPI
MS.gene42903	MS.gene048342	PPI
MS.gene81712	MS.gene048342	PPI
MS.gene021965	MS.gene048342	PPI
MS.gene055683	MS.gene048342	PPI
MS.gene042614	MS.gene048342	PPI
MS.gene41643	MS.gene048342	PPI
MS.gene26844	MS.gene048342	PPI
MS.gene021555	MS.gene048342	PPI
MS.gene048747	MS.gene048342	PPI
MS.gene021647	MS.gene048342	PPI
MS.gene69584	MS.gene048342	PPI
MS.gene019165	MS.gene048342	PPI
MS.gene048706	MS.gene048342	PPI
MS.gene048342	MS.gene015093	PPI
MS.gene055546	MS.gene048342	PPI
MS.gene048342	MS.gene000379	PPI
MS.gene009121	MS.gene048342	PPI
MS.gene94654	MS.gene048342	PPI
MS.gene073573	MS.gene048342	PPI
MS.gene031967	MS.gene048342	PPI
MS.gene87845	MS.gene048342	PPI
MS.gene048342	MS.gene08606	PPI
MS.gene063214	MS.gene048342	PPI
MS.gene97410	MS.gene048342	PPI
MS.gene048342	MS.gene09517	PPI
MS.gene83772	MS.gene048342	PPI
MS.gene32415	MS.gene048342	PPI
MS.gene009213	MS.gene048342	PPI
MS.gene060788	MS.gene048342	PPI
MS.gene035626	MS.gene048342	PPI
MS.gene008927	MS.gene048342	PPI
MS.gene045614	MS.gene048342	PPI
MS.gene63762	MS.gene048342	PPI
MS.gene048342	MS.gene62249	PPI
MS.gene27329	MS.gene048342	PPI
MS.gene71675	MS.gene048342	PPI
MS.gene30208	MS.gene048342	PPI
MS.gene028716	MS.gene048342	PPI
MS.gene21066	MS.gene048342	PPI
MS.gene69495	MS.gene048342	PPI
MS.gene013676	MS.gene048342	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048342.t1	MTR_3g053920	44.625	307	100	5	1	306	1	238	5.63e-82	253
MS.gene048342.t1	MTR_2g061500	57.364	129	55	0	1	129	1	129	1.32e-51	170
MS.gene048342.t1	MTR_6g033215	34.831	178	114	1	24	199	117	294	5.52e-37	137
MS.gene048342.t1	MTR_0010s0370	38.286	175	102	3	8	177	4	177	3.22e-35	129
MS.gene048342.t1	MTR_0010s0350	38.286	175	102	3	8	177	4	177	3.22e-35	129
MS.gene048342.t1	MTR_4g103530	58.427	89	37	0	59	147	5	93	1.10e-34	127
MS.gene048342.t1	MTR_7g011710	58.537	82	34	0	59	140	5	86	8.03e-31	116
MS.gene048342.t1	MTR_1g036720	37.963	108	65	1	7	112	3	110	3.42e-19	83.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene048342.t1	AT5G36228	26.943	193	137	2	36	226	39	229	2.39e-16	80.5
MS.gene048342.t1	AT2G41590	30.345	145	95	3	74	216	79	219	3.62e-16	80.1
MS.gene048342.t1	AT3G57587	30.345	145	95	3	74	216	79	219	1.15e-15	78.6
MS.gene048342.t1	AT2G17920	29.655	145	96	3	74	216	79	219	1.46e-15	77.4
MS.gene048342.t1	AT5G25200	28.873	142	97	2	77	216	80	219	1.70e-14	75.1
MS.gene048342.t1	AT5G18636	28.873	142	97	2	77	216	80	219	1.90e-14	74.7
MS.gene048342.t1	AT2G02103	29.577	142	96	2	77	216	80	219	2.25e-14	74.7
MS.gene048342.t1	AT3G31430	27.152	151	102	4	67	215	167	311	3.05e-14	73.9
MS.gene048342.t1	AT3G47920	29.655	145	96	3	74	216	8	148	3.83e-12	67.4
MS.gene048342.t1	AT2G13450	28.369	141	97	2	77	215	80	218	4.03e-12	67.8

Find 142 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTGAACCACCTTGATATT+TGG	0.150047	8.3:-34338718	MS.gene048342:CDS
GCTGGTTGCTAATGCTTCTT+TGG	0.203301	8.3:-34338150	MS.gene048342:CDS
CCCCTGTCTCAAATTGTTCT+TGG	0.248804	8.3:-34338077	MS.gene048342:CDS
AAGAACGCCTGGGCAATTTC+TGG	0.259448	8.3:-34338910	MS.gene048342:CDS
CTCTGTTATGGTGTCTTTGA+AGG	0.269602	8.3:-34338222	MS.gene048342:CDS
TTCTGCGCAGAGTGGTAATT+TGG	0.271351	8.3:-34338255	MS.gene048342:CDS
TGTTACCCAACCATTGAAAA+AGG	0.293474	8.3:-34338555	MS.gene048342:CDS
TGGAGGAGGACGATGGTGTT+AGG	0.295599	8.3:-34338403	MS.gene048342:CDS
GGCTGCTGGTTCCTCTGTTA+TGG	0.298869	8.3:-34338234	MS.gene048342:CDS
GGACGATGGTGTTAGGGGTT+GGG	0.307257	8.3:-34338396	MS.gene048342:CDS
CAGAGAGTGGCGAATGGAAT+TGG	0.309033	8.3:-34338659	MS.gene048342:CDS
CGCTACAAATGTCTGGTTGC+AGG	0.318919	8.3:-34338333	MS.gene048342:CDS
GACGATGGTGTTAGGGGTTG+GGG	0.319130	8.3:-34338395	MS.gene048342:CDS
TTTGAACCACCTTGATATTT+GGG	0.321438	8.3:-34338717	MS.gene048342:CDS
CCACCTTGATATTTGGGTTC+AGG	0.341780	8.3:-34338711	MS.gene048342:CDS
GCCCAGGCGTTCTTTGAAAT+AGG	0.341831	8.3:+34338919	None:intergenic
CAGGTACACCAGTTGCCTTT+TGG	0.354771	8.3:-34338692	MS.gene048342:CDS
ATCCTTGATCACTGTCTTGT+TGG	0.368740	8.3:-34338974	MS.gene048342:CDS
TGGTTCAAATTCACACTTGC+CGG	0.369574	8.3:+34338731	None:intergenic
GGAGGAGGACGATGGTGTTA+GGG	0.373898	8.3:-34338402	MS.gene048342:CDS
GTAATTGATACACGCTTTCC+AGG	0.376329	8.3:+34338884	None:intergenic
CTGCCCTCAGTTATTTGAAA+TGG	0.376439	8.3:-34338423	MS.gene048342:CDS
ATTTGTGTTTCGGCAGAGAC+TGG	0.380274	8.3:+34338995	None:intergenic
GTGATCAAGGATTTGTGTTT+CGG	0.381490	8.3:+34338985	None:intergenic
GTCTGTTGCTGATGGGGTCA+TGG	0.387152	8.3:-34337883	MS.gene048342:CDS
CTGGTTGCTAATGCTTCTTT+GGG	0.389166	8.3:-34338149	MS.gene048342:CDS
AGGACGATGGTGTTAGGGGT+TGG	0.397941	8.3:-34338397	MS.gene048342:CDS
TGCTATCAAAACTCAGTTGC+TGG	0.400440	8.3:-34338168	MS.gene048342:CDS
GAGGCCTCTTGTTCGACGTA+TGG	0.410600	8.3:-34338363	MS.gene048342:CDS
GTAGTTGATCGTATTGCTCC+CGG	0.419570	8.3:-34338761	MS.gene048342:CDS
AGTGCTCGTGTTCCTGCTGC+TGG	0.423241	8.3:-34338023	MS.gene048342:CDS
AGGGCTCTCACTGGAGTGGC+TGG	0.424848	8.3:-34337954	MS.gene048342:CDS
GGGGTTGGGGTGAAGCTTTG+AGG	0.425727	8.3:-34338382	MS.gene048342:CDS
GACAGGGGTGCTTCATTAAG+AGG	0.426249	8.3:+34338092	None:intergenic
TCTGTTGCTGATGGGGTCAT+GGG	0.427215	8.3:-34337882	MS.gene048342:CDS
TGCCTATTTCAAAGAACGCC+TGG	0.429628	8.3:-34338921	MS.gene048342:CDS
GCTTCCGACCTCAATCTTGA+AGG	0.432782	8.3:-34339058	MS.gene048342:CDS
CTGGGCAATTTCTGGCGGCC+TGG	0.432823	8.3:-34338902	MS.gene048342:CDS
TCGTATGTCTGCAGTGCATC+AGG	0.433786	8.3:-34338198	MS.gene048342:CDS
AATGGAATTGGGAGGTTCCT+TGG	0.435306	8.3:-34338647	MS.gene048342:CDS
GGACTAGAGCATTCACCTAC+TGG	0.441752	8.3:-34337933	MS.gene048342:CDS
GAGGTTCCTTGGTGAGTTGA+AGG	0.449014	8.3:-34338636	MS.gene048342:CDS
TCCTCAATGCTTCCAGCAGC+AGG	0.451565	8.3:+34338011	None:intergenic
TGTTACTTGTGTGGTCTGCT+TGG	0.454705	8.3:-34338461	MS.gene048342:CDS
AGAGAGTGGCGAATGGAATT+GGG	0.456005	8.3:-34338658	MS.gene048342:CDS
CATCAAGACGGTGATAGAAC+TGG	0.456183	8.3:+34338834	None:intergenic
TATTGCTCCCGGGGTGGTGC+CGG	0.458317	8.3:-34338750	MS.gene048342:CDS
GACGGTGATAGAACTGGAAC+AGG	0.458982	8.3:+34338840	None:intergenic
AGGCCTCTTGTTCGACGTAT+GGG	0.469191	8.3:-34338362	MS.gene048342:CDS
GTTATACGACAATTACAATC+TGG	0.469732	8.3:-34338783	MS.gene048342:CDS
GCGTGTATCAATTACACAAT+CGG	0.470625	8.3:-34338876	MS.gene048342:CDS
CAACCATACCAAAAGGCAAC+TGG	0.471652	8.3:+34338684	None:intergenic
GAGTTTGAGTCTGTTGCTGA+TGG	0.473346	8.3:-34337891	MS.gene048342:CDS
TAGGCAATATTGTTAGTGAT+GGG	0.473988	8.3:-34337848	MS.gene048342:CDS
CTTCCGACCTCAATCTTGAA+GGG	0.475423	8.3:-34339057	MS.gene048342:CDS
TGAGAGTTTCGATTCGGTAT+CGG	0.476356	8.3:+34338308	None:intergenic
GGCCAAGAACAATTTGAGAC+AGG	0.478602	8.3:+34338075	None:intergenic
TCAAATTGTTCTTGGCCTGC+CGG	0.483747	8.3:-34338069	MS.gene048342:CDS
TCTGTTATGGTGTCTTTGAA+GGG	0.486899	8.3:-34338221	MS.gene048342:CDS
TAGTTGATCGTATTGCTCCC+GGG	0.491508	8.3:-34338760	MS.gene048342:CDS
GGCAATATTGTTAGTGATGG+GGG	0.493540	8.3:-34337846	MS.gene048342:CDS
GGGTTTACCTTGACTGATGA+GGG	0.494034	8.3:-34339037	MS.gene048342:CDS
ACACTTGCCGGCACCACCCC+GGG	0.494936	8.3:+34338743	None:intergenic
GTAGGCAATATTGTTAGTGA+TGG	0.496033	8.3:-34337849	MS.gene048342:CDS
CTGAATTGAAGAAGCGCAAG+AGG	0.496918	8.3:-34337974	MS.gene048342:CDS
CAGAGTGGTAATTTGGCTGC+TGG	0.497800	8.3:-34338248	MS.gene048342:CDS
TCTCATGTTGCACATAGTAC+TGG	0.501221	8.3:-34338290	MS.gene048342:CDS
CACACTTGCCGGCACCACCC+CGG	0.508347	8.3:+34338742	None:intergenic
GCCTATTTCAAAGAACGCCT+GGG	0.511829	8.3:-34338920	MS.gene048342:CDS
AAGCGCAAGAGGGCTCTCAC+TGG	0.513053	8.3:-34337963	MS.gene048342:CDS
CAGAGACTGGGTCAAGGTCA+AGG	0.514598	8.3:+34339008	None:intergenic
CTCTGTTCAACCATACCAAA+AGG	0.515790	8.3:+34338677	None:intergenic
GTCATGGGGAACACTCTTGT+AGG	0.517311	8.3:-34337867	MS.gene048342:CDS
AGTTTGAGTCTGTTGCTGAT+GGG	0.517382	8.3:-34337890	MS.gene048342:CDS
CGTGTATCAATTACACAATC+GGG	0.519074	8.3:-34338875	MS.gene048342:CDS
AAATTTAAGTATGAGAAACT+TGG	0.521950	8.3:-34338491	MS.gene048342:CDS
GACCAACAAGACAGTGATCA+AGG	0.525759	8.3:+34338972	None:intergenic
TCATTAAGAGGTAGCAGAGA+AGG	0.526992	8.3:+34338104	None:intergenic
CCCTCAGTTATTTGAAATGG+AGG	0.533704	8.3:-34338420	MS.gene048342:CDS
GTACTGCCGCTACAAATGTC+TGG	0.535659	8.3:-34338340	MS.gene048342:CDS
GCAACATGAGAGTTTCGATT+CGG	0.535989	8.3:+34338302	None:intergenic
CAGCTCATATATGCGGTTGA+AGG	0.536342	8.3:-34338588	MS.gene048342:CDS
AGTTGAAGGAATATGACAAA+AGG	0.536923	8.3:-34338622	MS.gene048342:CDS
AGGTCCTTGATGAGGGCCCT+TGG	0.545130	8.3:-34338805	MS.gene048342:CDS
GCAGCTTGTGTTCCTCCAGT+AGG	0.551501	8.3:+34337918	None:intergenic
TTTGTGTTTCGGCAGAGACT+GGG	0.556028	8.3:+34338996	None:intergenic
CAGGCCGCCAGAAATTGCCC+AGG	0.561745	8.3:+34338903	None:intergenic
AGGGTTTACCTTGACTGATG+AGG	0.562347	8.3:-34339038	MS.gene048342:CDS
ATCAGCAACAGACTCAAACT+CGG	0.562939	8.3:+34337893	None:intergenic
CATTACCACTCACCGCCGGC+AGG	0.564369	8.3:+34338054	None:intergenic
CTGTTGCTGATGGGGTCATG+GGG	0.567642	8.3:-34337881	MS.gene048342:CDS
CTTGGCCTGCCGGCGGTGAG+TGG	0.567844	8.3:-34338059	MS.gene048342:CDS
TCACCGTCTTGATGCTGCGA+AGG	0.568804	8.3:-34338825	MS.gene048342:CDS
AGGTAAACCCTTCAAGATTG+AGG	0.573350	8.3:+34339050	None:intergenic
TGGCAGGTGCGAATCAAAGA+TGG	0.575606	8.3:-34338530	MS.gene048342:CDS
TCCTGCTGCTGGAAGCATTG+AGG	0.577183	8.3:-34338012	MS.gene048342:CDS
TCAGTTATTTGAAATGGAGG+AGG	0.577606	8.3:-34338417	MS.gene048342:CDS
ATAACCAAGGGCCCTCATCA+AGG	0.577759	8.3:+34338801	None:intergenic
CAAGAGGGCTCTCACTGGAG+TGG	0.581303	8.3:-34337958	MS.gene048342:CDS
TCAGCAACAGACTCAAACTC+GGG	0.583374	8.3:+34337894	None:intergenic
TGGTATGGTTGAACAGAGAG+TGG	0.583634	8.3:-34338672	MS.gene048342:CDS
TTTGAAATGGAGGAGGACGA+TGG	0.583723	8.3:-34338410	MS.gene048342:CDS
GTACCTGAACCCAAATATCA+AGG	0.586054	8.3:+34338708	None:intergenic
CAATATTGTTAGTGATGGGG+GGG	0.589744	8.3:-34337844	MS.gene048342:CDS
GTTTGAGTCTGTTGCTGATG+GGG	0.594723	8.3:-34337889	MS.gene048342:CDS
AGAGACTGGGTCAAGGTCAA+GGG	0.595115	8.3:+34339009	None:intergenic
AATTGTTCTTGGCCTGCCGG+CGG	0.598129	8.3:-34338066	MS.gene048342:CDS
CCTCCATTTCAAATAACTGA+GGG	0.599605	8.3:+34338420	None:intergenic
GCCAAGAACAATTTGAGACA+GGG	0.601325	8.3:+34338076	None:intergenic
GCTGCGAAGGTCCTTGATGA+GGG	0.603974	8.3:-34338812	MS.gene048342:CDS
TGCTGCGAAGGTCCTTGATG+AGG	0.604949	8.3:-34338813	MS.gene048342:CDS
GAGGAGGACGATGGTGTTAG+GGG	0.610517	8.3:-34338401	MS.gene048342:CDS
AGGCAATATTGTTAGTGATG+GGG	0.612373	8.3:-34337847	MS.gene048342:CDS
TTTCGGCAGAGACTGGGTCA+AGG	0.623168	8.3:+34339002	None:intergenic
AGCATTGAGGATGGCATACA+CGG	0.623705	8.3:-34337999	MS.gene048342:CDS
GTTGAACAGAGAGTGGCGAA+TGG	0.624745	8.3:-34338665	MS.gene048342:CDS
GCTGCTGGAAGCATTGAGGA+TGG	0.631278	8.3:-34338008	MS.gene048342:CDS
AGTTGATCGTATTGCTCCCG+GGG	0.633505	8.3:-34338759	MS.gene048342:CDS
TCCTCCATTTCAAATAACTG+AGG	0.633808	8.3:+34338419	None:intergenic
GTACCCATACGTCGAACAAG+AGG	0.634253	8.3:+34338359	None:intergenic
TTGTAATTGTCGTATAACCA+AGG	0.635037	8.3:+34338788	None:intergenic
AAACCCTTCAAGATTGAGGT+CGG	0.637161	8.3:+34339054	None:intergenic
GCAATATTGTTAGTGATGGG+GGG	0.642558	8.3:-34337845	MS.gene048342:CDS
AACGCCTGGGCAATTTCTGG+CGG	0.642790	8.3:-34338907	MS.gene048342:CDS
GGTTAACCCCTCATCAGTCA+AGG	0.643562	8.3:+34339030	None:intergenic
TGTAATTGTCGTATAACCAA+GGG	0.644854	8.3:+34338789	None:intergenic
GTTGCACATAGTACTGGAGT+AGG	0.646435	8.3:-34338284	MS.gene048342:CDS
GGACCTTCGCAGCATCAAGA+CGG	0.649361	8.3:+34338822	None:intergenic
TCAGCATTACCACTCACCGC+CGG	0.653940	8.3:+34338050	None:intergenic
CATATTCCTTCAACTCACCA+AGG	0.656330	8.3:+34338630	None:intergenic
ATCTGCACAGCTCATATATG+CGG	0.658404	8.3:-34338595	MS.gene048342:CDS
CCAAGAACAATTTGAGACAG+GGG	0.658985	8.3:+34338077	None:intergenic
GGTTTACCTTGACTGATGAG+GGG	0.661583	8.3:-34339036	MS.gene048342:CDS
TGATCGTATTGCTCCCGGGG+TGG	0.670048	8.3:-34338756	MS.gene048342:CDS
CTAGAGCATTCACCTACTGG+AGG	0.677525	8.3:-34337930	MS.gene048342:CDS
GAGTGGCGAATGGAATTGGG+AGG	0.678855	8.3:-34338655	MS.gene048342:CDS
TGAATTGAAGAAGCGCAAGA+GGG	0.681242	8.3:-34337973	MS.gene048342:CDS
GGCTCTACTTCTGCGCAGAG+TGG	0.682275	8.3:-34338263	MS.gene048342:CDS
CTGCAACCAGACATTTGTAG+CGG	0.689100	8.3:+34338334	None:intergenic
AGCGAATCTGCTTATCAGTG+AGG	0.690700	8.3:+34338945	None:intergenic
CTTCAAAGACACCATAACAG+AGG	0.715976	8.3:+34338223	None:intergenic
CCTGAACCCAAATATCAAGG+TGG	0.745016	8.3:+34338711	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTTAAGTATGAGAAACT+TGG	-	chr8.3:34337689-34337708	MS.gene048342:intron	20.0%
!	CCAAAATCATATTACTGTTT+CGG	-	chr8.3:34338695-34338714	MS.gene048342:CDS	25.0%
!	TACTTTATTCTTGTGAACTA+AGG	+	chr8.3:34338556-34338575	None:intergenic	25.0%
!!	CGAAACAGTAATATGATTTT+GGG	+	chr8.3:34338697-34338716	None:intergenic	25.0%
!!	TGTTTTTCTGTAGATAGTAT+TGG	-	chr8.3:34338609-34338628	MS.gene048342:CDS	25.0%
	AGTTCACAAGAATAAAGTAG+AGG	-	chr8.3:34338557-34338576	MS.gene048342:CDS	30.0%
	AGTTGAAGGAATATGACAAA+AGG	-	chr8.3:34337558-34337577	MS.gene048342:intron	30.0%
	ATTTGAACCACCTTGATATT+TGG	-	chr8.3:34337462-34337481	MS.gene048342:intron	30.0%
	CATTTAGCTAGACAAATTAC+TGG	-	chr8.3:34338809-34338828	MS.gene048342:CDS	30.0%
	GTATGATGAAACTGATGTAA+TGG	-	chr8.3:34338390-34338409	MS.gene048342:CDS	30.0%
	GTTATACGACAATTACAATC+TGG	-	chr8.3:34337397-34337416	MS.gene048342:intron	30.0%
	TAGGCAATATTGTTAGTGAT+GGG	-	chr8.3:34338332-34338351	MS.gene048342:CDS	30.0%
	TGTAATTGTCGTATAACCAA+GGG	+	chr8.3:34337394-34337413	None:intergenic	30.0%
	TTGTAATTGTCGTATAACCA+AGG	+	chr8.3:34337395-34337414	None:intergenic	30.0%
	TTTGAACCACCTTGATATTT+GGG	-	chr8.3:34337463-34337482	MS.gene048342:intron	30.0%
!	CCGAAACAGTAATATGATTT+TGG	+	chr8.3:34338698-34338717	None:intergenic	30.0%
!!	ATTGGCTTATCAATGGTTTT+CGG	-	chr8.3:34338906-34338925	MS.gene048342:CDS	30.0%
!!	CGGAGATTTTAATGACATAA+TGG	-	chr8.3:34338847-34338866	MS.gene048342:CDS	30.0%
!!	GGTATATTTTGTTACTTGTG+TGG	-	chr8.3:34337710-34337729	MS.gene048342:intron	30.0%
!!	TAATGGATTCTAGTGAGAAA+CGG	-	chr8.3:34338864-34338883	MS.gene048342:CDS	30.0%
	ACATCAGTTTCATCATACAG+TGG	+	chr8.3:34338388-34338407	None:intergenic	35.0%
	AGAAACGGTGTAGAAATTGT+AGG	-	chr8.3:34338879-34338898	MS.gene048342:CDS	35.0%
	AGGCAATATTGTTAGTGATG+GGG	-	chr8.3:34338333-34338352	MS.gene048342:CDS	35.0%
	CCTCCATTTCAAATAACTGA+GGG	+	chr8.3:34337763-34337782	None:intergenic	35.0%
	CGTGTATCAATTACACAATC+GGG	-	chr8.3:34337305-34337324	MS.gene048342:intron	35.0%
	CTTAACAGACTCATTTACCT+CGG	+	chr8.3:34338438-34338457	None:intergenic	35.0%
	GCGTGTATCAATTACACAAT+CGG	-	chr8.3:34337304-34337323	MS.gene048342:intron	35.0%
	GGTAAATGAGTCTGTTAAGT+TGG	-	chr8.3:34338439-34338458	MS.gene048342:CDS	35.0%
	GTAGAAATTGTAGGTCGAAT+TGG	-	chr8.3:34338888-34338907	MS.gene048342:CDS	35.0%
	GTAGGCAATATTGTTAGTGA+TGG	-	chr8.3:34338331-34338350	MS.gene048342:CDS	35.0%
	GTGATCAAGGATTTGTGTTT+CGG	+	chr8.3:34337198-34337217	None:intergenic	35.0%
	TCCTCCATTTCAAATAACTG+AGG	+	chr8.3:34337764-34337783	None:intergenic	35.0%
	TCTGTTATGGTGTCTTTGAA+GGG	-	chr8.3:34337959-34337978	MS.gene048342:CDS	35.0%
	TGCAGGAAATAAAGAAAACC+AGG	+	chr8.3:34339056-34339075	None:intergenic	35.0%
	TGTTACCCAACCATTGAAAA+AGG	-	chr8.3:34337625-34337644	MS.gene048342:intron	35.0%
!	ATCTGAAATACCTTGTACGT+CGG	-	chr8.3:34338499-34338518	MS.gene048342:CDS	35.0%
!	TCAGTTATTTGAAATGGAGG+AGG	-	chr8.3:34337763-34337782	MS.gene048342:intron	35.0%
!!	GTAGATAGTATTGGTAGAAG+TGG	-	chr8.3:34338618-34338637	MS.gene048342:CDS	35.0%
	AAACCCTTCAAGATTGAGGT+CGG	+	chr8.3:34337129-34337148	None:intergenic	40.0%
	AAATCTCCGAAGATACACCA+AGG	+	chr8.3:34338836-34338855	None:intergenic	40.0%
	AGGTAAACCCTTCAAGATTG+AGG	+	chr8.3:34337133-34337152	None:intergenic	40.0%
	AGTTTGAGTCTGTTGCTGAT+GGG	-	chr8.3:34338290-34338309	MS.gene048342:CDS	40.0%
	ATCAGCAACAGACTCAAACT+CGG	+	chr8.3:34338290-34338309	None:intergenic	40.0%
	ATCCTTGATCACTGTCTTGT+TGG	-	chr8.3:34337206-34337225	MS.gene048342:intron	40.0%
	ATCTGCACAGCTCATATATG+CGG	-	chr8.3:34337585-34337604	MS.gene048342:intron	40.0%
	CATATTCCTTCAACTCACCA+AGG	+	chr8.3:34337553-34337572	None:intergenic	40.0%
	CGGCAATCAGTAATTAACTC+TGG	-	chr8.3:34338926-34338945	MS.gene048342:CDS	40.0%
	CTAGACAAATTACTGGTCCT+TGG	-	chr8.3:34338816-34338835	MS.gene048342:CDS	40.0%
	CTCTGTTATGGTGTCTTTGA+AGG	-	chr8.3:34337958-34337977	MS.gene048342:CDS	40.0%
	CTCTGTTCAACCATACCAAA+AGG	+	chr8.3:34337506-34337525	None:intergenic	40.0%
	CTGATGCTGTTCATGGTAAT+TGG	-	chr8.3:34338721-34338740	MS.gene048342:CDS	40.0%
	CTGCCCTCAGTTATTTGAAA+TGG	-	chr8.3:34337757-34337776	MS.gene048342:intron	40.0%
	CTGGTTGCTAATGCTTCTTT+GGG	-	chr8.3:34338031-34338050	MS.gene048342:CDS	40.0%
	CTTCAAAGACACCATAACAG+AGG	+	chr8.3:34337960-34337979	None:intergenic	40.0%
	GAGGATTTGCGTTATGTTCT+TGG	-	chr8.3:34338576-34338595	MS.gene048342:CDS	40.0%
	GATGAAACTGATGTAATGGC+AGG	-	chr8.3:34338394-34338413	MS.gene048342:CDS	40.0%
	GCAACATGAGAGTTTCGATT+CGG	+	chr8.3:34337881-34337900	None:intergenic	40.0%
	GGCAATATTGTTAGTGATGG+GGG	-	chr8.3:34338334-34338353	MS.gene048342:CDS	40.0%
	GTAATTGATACACGCTTTCC+AGG	+	chr8.3:34337299-34337318	None:intergenic	40.0%
	GTACCTGAACCCAAATATCA+AGG	+	chr8.3:34337475-34337494	None:intergenic	40.0%
	TAGCACGCCAATAAATAGCA+AGG	+	chr8.3:34338647-34338666	None:intergenic	40.0%
	TCATTAAGAGGTAGCAGAGA+AGG	+	chr8.3:34338079-34338098	None:intergenic	40.0%
	TCTCATGTTGCACATAGTAC+TGG	-	chr8.3:34337890-34337909	MS.gene048342:CDS	40.0%
	TGCTATCAAAACTCAGTTGC+TGG	-	chr8.3:34338012-34338031	MS.gene048342:CDS	40.0%
	TGGTTCAAATTCACACTTGC+CGG	+	chr8.3:34337452-34337471	None:intergenic	40.0%
	TGTGAACTAAGGTCTCACTT+AGG	+	chr8.3:34338545-34338564	None:intergenic	40.0%
	TTGAGGGATATCCATACACT+TGG	-	chr8.3:34338966-34338985	MS.gene048342:CDS	40.0%
!	AGGTCGAATTGGCTTATCAA+TGG	-	chr8.3:34338899-34338918	MS.gene048342:CDS	40.0%
!	CAATATTGTTAGTGATGGGG+GGG	-	chr8.3:34338336-34338355	MS.gene048342:CDS	40.0%
!	CCAAGAACAATTTGAGACAG+GGG	+	chr8.3:34338106-34338125	None:intergenic	40.0%
!	CCCTCAGTTATTTGAAATGG+AGG	-	chr8.3:34337760-34337779	MS.gene048342:intron	40.0%
!	CGTACAAGGTATTTCAGATC+AGG	+	chr8.3:34338498-34338517	None:intergenic	40.0%
!	GCAATATTGTTAGTGATGGG+GGG	-	chr8.3:34338335-34338354	MS.gene048342:CDS	40.0%
!	GCCAAGAACAATTTGAGACA+GGG	+	chr8.3:34338107-34338126	None:intergenic	40.0%
!	TGAATTGAAGAAGCGCAAGA+GGG	-	chr8.3:34338207-34338226	MS.gene048342:CDS	40.0%
!!	GATAGTATTGGTAGAAGTGG+TGG	-	chr8.3:34338621-34338640	MS.gene048342:CDS	40.0%
!!	GTCTGTTAAGTTGGAACTGT+CGG	-	chr8.3:34338448-34338467	MS.gene048342:CDS	40.0%
!!	TCTGTTAAGTTGGAACTGTC+GGG	-	chr8.3:34338449-34338468	MS.gene048342:CDS	40.0%
!!	TGAGAGTTTCGATTCGGTAT+CGG	+	chr8.3:34337875-34337894	None:intergenic	40.0%
!!!	CCACTCCTTTTTCAATGGTT+GGG	+	chr8.3:34337633-34337652	None:intergenic	40.0%
	AATGGAATTGGGAGGTTCCT+TGG	-	chr8.3:34337533-34337552	MS.gene048342:intron	45.0%
	ACCATTGAAAAAGGAGTGGC+AGG	-	chr8.3:34337634-34337653	MS.gene048342:intron	45.0%
	AGAGAGTGGCGAATGGAATT+GGG	-	chr8.3:34337522-34337541	MS.gene048342:intron	45.0%
	AGCGAATCTGCTTATCAGTG+AGG	+	chr8.3:34337238-34337257	None:intergenic	45.0%
	AGGCTCTTGAACCAAGTGTA+TGG	+	chr8.3:34338980-34338999	None:intergenic	45.0%
	AGGGTTTACCTTGACTGATG+AGG	-	chr8.3:34337142-34337161	MS.gene048342:intron	45.0%
	ATACACTTGGTTCAAGAGCC+TGG	-	chr8.3:34338979-34338998	MS.gene048342:CDS	45.0%
	ATTTGTGTTTCGGCAGAGAC+TGG	+	chr8.3:34337188-34337207	None:intergenic	45.0%
	CAACCATACCAAAAGGCAAC+TGG	+	chr8.3:34337499-34337518	None:intergenic	45.0%
	CAGCTCATATATGCGGTTGA+AGG	-	chr8.3:34337592-34337611	MS.gene048342:intron	45.0%
	CATCAAGACGGTGATAGAAC+TGG	+	chr8.3:34337349-34337368	None:intergenic	45.0%
	CCCAACCATTGAAAAAGGAG+TGG	-	chr8.3:34337630-34337649	MS.gene048342:intron	45.0%
	CCCCTGTCTCAAATTGTTCT+TGG	-	chr8.3:34338103-34338122	MS.gene048342:CDS	45.0%
	CCTGAACCCAAATATCAAGG+TGG	+	chr8.3:34337472-34337491	None:intergenic	45.0%
	CTAAGGTCTCACTTAGGAAG+AGG	+	chr8.3:34338539-34338558	None:intergenic	45.0%
	CTGCAACCAGACATTTGTAG+CGG	+	chr8.3:34337849-34337868	None:intergenic	45.0%
	CTTCCGACCTCAATCTTGAA+GGG	-	chr8.3:34337123-34337142	MS.gene048342:intron	45.0%
	GACCAACAAGACAGTGATCA+AGG	+	chr8.3:34337211-34337230	None:intergenic	45.0%
	GAGTTTGAGTCTGTTGCTGA+TGG	-	chr8.3:34338289-34338308	MS.gene048342:CDS	45.0%
	GCCTATTTCAAAGAACGCCT+GGG	-	chr8.3:34337260-34337279	MS.gene048342:intron	45.0%
	GCTGGTTGCTAATGCTTCTT+TGG	-	chr8.3:34338030-34338049	MS.gene048342:CDS	45.0%
	GGGTTTACCTTGACTGATGA+GGG	-	chr8.3:34337143-34337162	MS.gene048342:intron	45.0%
	GGTTTACCTTGACTGATGAG+GGG	-	chr8.3:34337144-34337163	MS.gene048342:intron	45.0%
	GTAGTTGATCGTATTGCTCC+CGG	-	chr8.3:34337419-34337438	MS.gene048342:intron	45.0%
	GTGTATGGATATCCCTCAAC+AGG	+	chr8.3:34338965-34338984	None:intergenic	45.0%
	GTTTGAGTCTGTTGCTGATG+GGG	-	chr8.3:34338291-34338310	MS.gene048342:CDS	45.0%
	TACACTTGGTTCAAGAGCCT+GGG	-	chr8.3:34338980-34338999	MS.gene048342:CDS	45.0%
	TAGTTGATCGTATTGCTCCC+GGG	-	chr8.3:34337420-34337439	MS.gene048342:intron	45.0%
	TCAAATTGTTCTTGGCCTGC+CGG	-	chr8.3:34338111-34338130	MS.gene048342:CDS	45.0%
	TCAGCAACAGACTCAAACTC+GGG	+	chr8.3:34338289-34338308	None:intergenic	45.0%
	TGCCTATTTCAAAGAACGCC+TGG	-	chr8.3:34337259-34337278	MS.gene048342:intron	45.0%
	TGGTATGGTTGAACAGAGAG+TGG	-	chr8.3:34337508-34337527	MS.gene048342:intron	45.0%
	TTCTGCGCAGAGTGGTAATT+TGG	-	chr8.3:34337925-34337944	MS.gene048342:CDS	45.0%
	TTTGTGTTTCGGCAGAGACT+GGG	+	chr8.3:34337187-34337206	None:intergenic	45.0%
!	ACACCAGTTGCCTTTTGGTA+TGG	-	chr8.3:34337493-34337512	MS.gene048342:intron	45.0%
!	ACTGGTCCTTGGTGTATCTT+CGG	-	chr8.3:34338827-34338846	MS.gene048342:CDS	45.0%
!	AGCATTGAGGATGGCATACA+CGG	-	chr8.3:34338181-34338200	MS.gene048342:CDS	45.0%
!	CCACCTTGATATTTGGGTTC+AGG	-	chr8.3:34337469-34337488	MS.gene048342:intron	45.0%
!	CTGAATTGAAGAAGCGCAAG+AGG	-	chr8.3:34338206-34338225	MS.gene048342:CDS	45.0%
!	GGCCAAGAACAATTTGAGAC+AGG	+	chr8.3:34338108-34338127	None:intergenic	45.0%
!	GTTGCACATAGTACTGGAGT+AGG	-	chr8.3:34337896-34337915	MS.gene048342:CDS	45.0%
!	TGTTACTTGTGTGGTCTGCT+TGG	-	chr8.3:34337719-34337738	MS.gene048342:intron	45.0%
!	TTTGAAATGGAGGAGGACGA+TGG	-	chr8.3:34337770-34337789	MS.gene048342:intron	45.0%
!!	CCTTGTACGTCGGTTTAATC+CGG	-	chr8.3:34338509-34338528	MS.gene048342:CDS	45.0%
!!	CTGTTAAGTTGGAACTGTCG+GGG	-	chr8.3:34338450-34338469	MS.gene048342:CDS	45.0%
!!	GTGGTGGCCTTGCTATTTAT+TGG	-	chr8.3:34338637-34338656	MS.gene048342:CDS	45.0%
!!!	ACCTGCCACTCCTTTTTCAA+TGG	+	chr8.3:34337638-34337657	None:intergenic	45.0%
!!!	GCCACTCCTTTTTCAATGGT+TGG	+	chr8.3:34337634-34337653	None:intergenic	45.0%
	AAGAACGCCTGGGCAATTTC+TGG	-	chr8.3:34337270-34337289	MS.gene048342:intron	50.0%
	AATCTTTCCTCAACAGCCCG+AGG	+	chr8.3:34339010-34339029	None:intergenic	50.0%
	ACAGACTCATTTACCTCGGC+AGG	+	chr8.3:34338434-34338453	None:intergenic	50.0%
	AGAGACTGGGTCAAGGTCAA+GGG	+	chr8.3:34337174-34337193	None:intergenic	50.0%
	AGGCCTCTTGTTCGACGTAT+GGG	-	chr8.3:34337818-34337837	MS.gene048342:CDS	50.0%
	AGTTGATCGTATTGCTCCCG+GGG	-	chr8.3:34337421-34337440	MS.gene048342:intron	50.0%
	AGTTGGAACTGTCGGGGTAT+AGG	-	chr8.3:34338456-34338475	MS.gene048342:CDS	50.0%
	ATAACCAAGGGCCCTCATCA+AGG	+	chr8.3:34337382-34337401	None:intergenic	50.0%
	CAGAGAGTGGCGAATGGAAT+TGG	-	chr8.3:34337521-34337540	MS.gene048342:intron	50.0%
	CAGAGTGGTAATTTGGCTGC+TGG	-	chr8.3:34337932-34337951	MS.gene048342:CDS	50.0%
	CCGGATTAAACCGACGTACA+AGG	+	chr8.3:34338512-34338531	None:intergenic	50.0%
	CGCTACAAATGTCTGGTTGC+AGG	-	chr8.3:34337847-34337866	MS.gene048342:CDS	50.0%
	CTCTCTGATGTGCCTGTTGA+GGG	-	chr8.3:34338950-34338969	MS.gene048342:CDS	50.0%
	GACGGTGATAGAACTGGAAC+AGG	+	chr8.3:34337343-34337362	None:intergenic	50.0%
	GCTTCCGACCTCAATCTTGA+AGG	-	chr8.3:34337122-34337141	MS.gene048342:intron	50.0%
	GGTTAACCCCTCATCAGTCA+AGG	+	chr8.3:34337153-34337172	None:intergenic	50.0%
	GTACCCATACGTCGAACAAG+AGG	+	chr8.3:34337824-34337843	None:intergenic	50.0%
	GTACTGCCGCTACAAATGTC+TGG	-	chr8.3:34337840-34337859	MS.gene048342:CDS	50.0%
	GTCATGGGGAACACTCTTGT+AGG	-	chr8.3:34338313-34338332	MS.gene048342:CDS	50.0%
	GTTGAACAGAGAGTGGCGAA+TGG	-	chr8.3:34337515-34337534	MS.gene048342:intron	50.0%
	TCACTTAGGAAGAGGAGAGC+CGG	+	chr8.3:34338531-34338550	None:intergenic	50.0%
	TCGTATGTCTGCAGTGCATC+AGG	-	chr8.3:34337982-34338001	MS.gene048342:CDS	50.0%
	TCTGTTGCTGATGGGGTCAT+GGG	-	chr8.3:34338298-34338317	MS.gene048342:CDS	50.0%
	TGGAGTCTCACCTGCTACTA+CGG	-	chr8.3:34338741-34338760	MS.gene048342:CDS	50.0%
	TGGCAGGTGCGAATCAAAGA+TGG	-	chr8.3:34337650-34337669	MS.gene048342:intron	50.0%
	TTCAGATCAGGAACTGCGCT+CGG	+	chr8.3:34338486-34338505	None:intergenic	50.0%
!	AGGCGTTGTTAGCAAGTGCA+CGG	+	chr8.3:34339036-34339055	None:intergenic	50.0%
!	CAGGTACACCAGTTGCCTTT+TGG	-	chr8.3:34337488-34337507	MS.gene048342:intron	50.0%
!	CTAGAGCATTCACCTACTGG+AGG	-	chr8.3:34338250-34338269	MS.gene048342:CDS	50.0%
!	GAGGTTCCTTGGTGAGTTGA+AGG	-	chr8.3:34337544-34337563	MS.gene048342:intron	50.0%
!	GGACTAGAGCATTCACCTAC+TGG	-	chr8.3:34338247-34338266	MS.gene048342:CDS	50.0%
!	TCGGTGTCTGATGCTGTTCA+TGG	-	chr8.3:34338714-34338733	MS.gene048342:CDS	50.0%
!!	GACAGGGGTGCTTCATTAAG+AGG	+	chr8.3:34338091-34338110	None:intergenic	50.0%
!!	GCCCAGGCGTTCTTTGAAAT+AGG	+	chr8.3:34337264-34337283	None:intergenic	50.0%
	AATGGCAGGTCCTGCTAGTC+AGG	-	chr8.3:34338408-34338427	MS.gene048342:CDS	55.0%
	AATTGTTCTTGGCCTGCCGG+CGG	-	chr8.3:34338114-34338133	MS.gene048342:CDS	55.0%
	CAGAGACTGGGTCAAGGTCA+AGG	+	chr8.3:34337175-34337194	None:intergenic	55.0%
	CCTCAACAGGCACATCAGAG+AGG	+	chr8.3:34338952-34338971	None:intergenic	55.0%
	CCTCTCTGATGTGCCTGTTG+AGG	-	chr8.3:34338949-34338968	MS.gene048342:CDS	55.0%
	CTGTTGCTGATGGGGTCATG+GGG	-	chr8.3:34338299-34338318	MS.gene048342:CDS	55.0%
	GAGGCCTCTTGTTCGACGTA+TGG	-	chr8.3:34337817-34337836	MS.gene048342:CDS	55.0%
	GAGTGGCGAATGGAATTGGG+AGG	-	chr8.3:34337525-34337544	MS.gene048342:intron	55.0%
	GCAGCTTGTGTTCCTCCAGT+AGG	+	chr8.3:34338265-34338284	None:intergenic	55.0%
	GCTGCGAAGGTCCTTGATGA+GGG	-	chr8.3:34337368-34337387	MS.gene048342:intron	55.0%
	GGACCTTCGCAGCATCAAGA+CGG	+	chr8.3:34337361-34337380	None:intergenic	55.0%
	GTCTGTTGCTGATGGGGTCA+TGG	-	chr8.3:34338297-34338316	MS.gene048342:CDS	55.0%
	TCAAGAGCCTGGGTACTCCT+CGG	-	chr8.3:34338990-34339009	MS.gene048342:CDS	55.0%
	TCACCGTCTTGATGCTGCGA+AGG	-	chr8.3:34337355-34337374	MS.gene048342:intron	55.0%
	TCAGATCAGGAACTGCGCTC+GGG	+	chr8.3:34338485-34338504	None:intergenic	55.0%
	TCAGCATTACCACTCACCGC+CGG	+	chr8.3:34338133-34338152	None:intergenic	55.0%
	TCCTCAATGCTTCCAGCAGC+AGG	+	chr8.3:34338172-34338191	None:intergenic	55.0%
	TGCTACTACGGATACCCCGA+GGG	-	chr8.3:34338753-34338772	MS.gene048342:CDS	55.0%
	TGCTGCGAAGGTCCTTGATG+AGG	-	chr8.3:34337367-34337386	MS.gene048342:intron	55.0%
	TGGAGGAGGACGATGGTGTT+AGG	-	chr8.3:34337777-34337796	MS.gene048342:intron	55.0%
	TTTCGGCAGAGACTGGGTCA+AGG	+	chr8.3:34337181-34337200	None:intergenic	55.0%
!	AACGCCTGGGCAATTTCTGG+CGG	-	chr8.3:34337273-34337292	MS.gene048342:intron	55.0%
!	AGGACGATGGTGTTAGGGGT+TGG	-	chr8.3:34337783-34337802	MS.gene048342:intron	55.0%
!	GACGATGGTGTTAGGGGTTG+GGG	-	chr8.3:34337785-34337804	MS.gene048342:intron	55.0%
!	GAGGAGGACGATGGTGTTAG+GGG	-	chr8.3:34337779-34337798	MS.gene048342:intron	55.0%
!	GGACGATGGTGTTAGGGGTT+GGG	-	chr8.3:34337784-34337803	MS.gene048342:intron	55.0%
!	GGAGGAGGACGATGGTGTTA+GGG	-	chr8.3:34337778-34337797	MS.gene048342:intron	55.0%
!	GTGCACTTGCTAACAACGCC+TGG	-	chr8.3:34339035-34339054	MS.gene048342:CDS	55.0%
!!	GCTGCTGGAAGCATTGAGGA+TGG	-	chr8.3:34338172-34338191	MS.gene048342:CDS	55.0%
!!	GGCTGCTGGTTCCTCTGTTA+TGG	-	chr8.3:34337946-34337965	MS.gene048342:CDS	55.0%
!!	TCCTGCTGCTGGAAGCATTG+AGG	-	chr8.3:34338168-34338187	MS.gene048342:CDS	55.0%
	AAGCGCAAGAGGGCTCTCAC+TGG	-	chr8.3:34338217-34338236	MS.gene048342:CDS	60.0%
	AGGTCCTTGATGAGGGCCCT+TGG	-	chr8.3:34337375-34337394	MS.gene048342:intron	60.0%
	AGTGCTCGTGTTCCTGCTGC+TGG	-	chr8.3:34338157-34338176	MS.gene048342:CDS	60.0%
	CAAGAGCCTGGGTACTCCTC+GGG	-	chr8.3:34338991-34339010	MS.gene048342:CDS	60.0%
	CAAGAGGGCTCTCACTGGAG+TGG	-	chr8.3:34338222-34338241	MS.gene048342:CDS	60.0%
	CTACTACGGATACCCCGAGG+GGG	-	chr8.3:34338755-34338774	MS.gene048342:CDS	60.0%
	CTGCTACTACGGATACCCCG+AGG	-	chr8.3:34338752-34338771	MS.gene048342:CDS	60.0%
	GCTACTACGGATACCCCGAG+GGG	-	chr8.3:34338754-34338773	MS.gene048342:CDS	60.0%
	GGCTCTACTTCTGCGCAGAG+TGG	-	chr8.3:34337917-34337936	MS.gene048342:CDS	60.0%
	GGGGTTGGGGTGAAGCTTTG+AGG	-	chr8.3:34337798-34337817	MS.gene048342:intron	60.0%
	TGATCGTATTGCTCCCGGGG+TGG	-	chr8.3:34337424-34337443	MS.gene048342:intron	60.0%
!	CTCGGGGTATCCGTAGTAGC+AGG	+	chr8.3:34338754-34338773	None:intergenic	60.0%
	AGGGCTCTCACTGGAGTGGC+TGG	-	chr8.3:34338226-34338245	MS.gene048342:CDS	65.0%
	CAGGCCGCCAGAAATTGCCC+AGG	+	chr8.3:34337280-34337299	None:intergenic	65.0%
	CATTACCACTCACCGCCGGC+AGG	+	chr8.3:34338129-34338148	None:intergenic	65.0%
	GGGTACTCCTCGGGCTGTTG+AGG	-	chr8.3:34339000-34339019	MS.gene048342:CDS	65.0%
	TATTGCTCCCGGGGTGGTGC+CGG	-	chr8.3:34337430-34337449	MS.gene048342:intron	65.0%
!!	CAACAGCCCGAGGAGTACCC+AGG	+	chr8.3:34339000-34339019	None:intergenic	65.0%
!!	CTGGGCAATTTCTGGCGGCC+TGG	-	chr8.3:34337278-34337297	MS.gene048342:intron	65.0%
	ACACTTGCCGGCACCACCCC+GGG	+	chr8.3:34337440-34337459	None:intergenic	70.0%
	CACACTTGCCGGCACCACCC+CGG	+	chr8.3:34337441-34337460	None:intergenic	70.0%
	CCTCGGCAGGCCTGACTAGC+AGG	+	chr8.3:34338421-34338440	None:intergenic	70.0%
	CCTGCTAGTCAGGCCTGCCG+AGG	-	chr8.3:34338418-34338437	MS.gene048342:CDS	70.0%
	GGATACCCCGAGGGGGACGT+AGG	-	chr8.3:34338762-34338781	MS.gene048342:CDS	70.0%
	CCGAGGGGGACGTAGGCGTG+CGG	-	chr8.3:34338769-34338788	MS.gene048342:CDS	75.0%
	CCGCACGCCTACGTCCCCCT+CGG	+	chr8.3:34338772-34338791	None:intergenic	75.0%
	CGCACGCCTACGTCCCCCTC+GGG	+	chr8.3:34338771-34338790	None:intergenic	75.0%
	CTTGGCCTGCCGGCGGTGAG+TGG	-	chr8.3:34338121-34338140	MS.gene048342:CDS	75.0%
	GCACGCCTACGTCCCCCTCG+GGG	+	chr8.3:34338770-34338789	None:intergenic	75.0%
	GGGACGTAGGCGTGCGGCGT+GGG	-	chr8.3:34338775-34338794	MS.gene048342:CDS	75.0%
!	GGGGACGTAGGCGTGCGGCG+TGG	-	chr8.3:34338774-34338793	MS.gene048342:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	34337116	34339086	34337116	ID=MS.gene048342
chr8.3	mRNA	34337116	34339086	34337116	ID=MS.gene048342.t1;Parent=MS.gene048342
chr8.3	exon	34337813	34339086	34337813	ID=MS.gene048342.t1.exon1;Parent=MS.gene048342.t1
chr8.3	CDS	34337813	34339086	34337813	ID=cds.MS.gene048342.t1;Parent=MS.gene048342.t1
chr8.3	exon	34337116	34337125	34337116	ID=MS.gene048342.t1.exon2;Parent=MS.gene048342.t1
chr8.3	CDS	34337116	34337125	34337116	ID=cds.MS.gene048342.t1;Parent=MS.gene048342.t1

Gene Sequence

>MS.gene048342

ATGTCTGCTTCCGACCTCAATCTTGAAGGGTTTACCTTGACTGATGAGGGGTTAACCCTTGACCTTGACCCAGTCTCTGCCGAAACACAAATCCTTGATCACTGTCTTGTTGGTCGTGTCCTCACTGATAAGCAGATTCGCTTTGCCTATTTCAAAGAACGCCTGGGCAATTTCTGGCGGCCTGGAAAGCGTGTATCAATTACACAATCGGGCGAGAATCGCTTCCTGTTCCAGTTCTATCACCGTCTTGATGCTGCGAAGGTCCTTGATGAGGGCCCTTGGTTATACGACAATTACAATCTGGTAGTTGATCGTATTGCTCCCGGGGTGGTGCCGGCAAGTGTGAATTTGAACCACCTTGATATTTGGGTTCAGGTACACCAGTTGCCTTTTGGTATGGTTGAACAGAGAGTGGCGAATGGAATTGGGAGGTTCCTTGGTGAGTTGAAGGAATATGACAAAAGGAACAATCTGCACAGCTCATATATGCGGTTGAAGGTCAGAATTGATGTTACCCAACCATTGAAAAAGGAGTGGCAGGTGCGAATCAAAGATGGTAGCTTCGCAACAATCAAATTTAAGTATGAGAAACTTGGTATATTTTGTTACTTGTGTGGTCTGCTTGGTCACACAGATAAGAGCTGCCCTCAGTTATTTGAAATGGAGGAGGACGATGGTGTTAGGGGTTGGGGTGAAGCTTTGAGGCCTCTTGTTCGACGTATGGGTACTGCCGCTACAAATGTCTGGTTGCAGGATCCGATACCGAATCGAAACTCTCATGTTGCACATAGTACTGGAGTAGGCTCTACTTCTGCGCAGAGTGGTAATTTGGCTGCTGGTTCCTCTGTTATGGTGTCTTTGAAGGGTCGTATGTCTGCAGTGCATCAGGAAATTGATGCTATCAAAACTCAGTTGCTGGTTGCTAATGCTTCTTTGGGTGAGTCTTCTCGCATTACTTCACCTTCTCTGCTACCTCTTAATGAAGCACCCCTGTCTCAAATTGTTCTTGGCCTGCCGGCGGTGAGTGGTAATGCTGATGTTAGTGCTCGTGTTCCTGCTGCTGGAAGCATTGAGGATGGCATACACGGTACTGAATTGAAGAAGCGCAAGAGGGCTCTCACTGGAGTGGCTGGACTAGAGCATTCACCTACTGGAGGAACACAAGCTGCACCCGAGTTTGAGTCTGTTGCTGATGGGGTCATGGGGAACACTCTTGTAGGCAATATTGTTAGTGATGGGGGGGATGTTGTAATGACTGTTAACAATAATCCACTGTATGATGAAACTGATGTAATGGCAGGTCCTGCTAGTCAGGCCTGCCGAGGTAAATGAGTCTGTTAAGTTGGAACTGTCGGGGTATAGGTAGCCCGAGCGCAGTTCCTGATCTGAAATACCTTGTACGTCGGTTTAATCCGGCTCTCCTCTTCCTAAGTGAGACCTTAGTTCACAAGAATAAAGTAGAGGATTTGCGTTATGTTCTTGGCTTTGATTTTTGTTTTTCTGTAGATAGTATTGGTAGAAGTGGTGGCCTTGCTATTTATTGGCGTGCTAACTTTAATTGTCAAATTGTTAACTACTCCCAAAATCATATTACTGTTTCGGTGTCTGATGCTGTTCATGGTAATTGGAGTCTCACCTGCTACTACGGATACCCCGAGGGGGACGTAGGCGTGCGGCGTGGGATTTTCTTCGTCATTTAGCTAGACAAATTACTGGTCCTTGGTGTATCTTCGGAGATTTTAATGACATAATGGATTCTAGTGAGAAACGGTGTAGAAATTGTAGGTCGAATTGGCTTATCAATGGTTTTCGGCAATCAGTAATTAACTCTGGCCTCTCTGATGTGCCTGTTGAGGGATATCCATACACTTGGTTCAAGAGCCTGGGTACTCCTCGGGCTGTTGAGGAAAGATTAGACCGTGCACTTGCTAACAACGCCTGGTTTTCTTTATTTCCTGCAGCAACTCTTGA

Protein sequence

>MS.gene048342.t1

MSASDLNLEGFTLTDEGLTLDLDPVSAETQILDHCLVGRVLTDKQIRFAYFKERLGNFWRPGKRVSITQSGENRFLFQFYHRLDAAKVLDEGPWLYDNYNLVVDRIAPGVVPASVNLNHLDIWVQVHQLPFGMVEQRVANGIGRFLGELKEYDKRNNLHSSYMRLKVRIDVTQPLKKEWQVRIKDGSFATIKFKYEKLGIFCYLCGLLGHTDKSCPQLFEMEEDDGVRGWGEALRPLVRRMGTAATNVWLQDPIPNRNSHVAHSTGVGSTSAQSGNLAAGSSVMVSLKGRMSAVHQEIDAIKTQLLVANASLGESSRITSPSLLPLNEAPLSQIVLGLPAVSGNADVSARVPAAGSIEDGIHGTELKKRKRALTGVAGLEHSPTGGTQAAPEFESVADGVMGNTLVGNIVSDGGDVVMTVNNNPLNS