AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32415.t1 MTR_3g053920 58.007 281 108 2 57 328 1 280 2.32e-105 312
MS.gene32415.t1 MTR_2g061500 75.969 129 31 0 57 185 1 129 1.29e-69 216
MS.gene32415.t1 MTR_4g103530 51.337 187 83 2 111 295 1 181 2.41e-54 178
MS.gene32415.t1 MTR_7g011710 74.038 104 23 1 111 210 1 104 1.51e-47 159
MS.gene32415.t1 MTR_6g033215 31.183 186 118 5 33 214 74 253 2.86e-21 93.6
MS.gene32415.t1 MTR_8g463110 47.826 115 60 0 271 385 1 115 3.62e-19 90.1
MS.gene32415.t1 MTR_0010s0370 29.921 127 84 1 88 209 30 156 2.92e-16 77.0
MS.gene32415.t1 MTR_0010s0350 29.921 127 84 1 88 209 30 156 2.92e-16 77.0
MS.gene32415.t1 MTR_1g036720 30.769 104 70 1 67 168 7 110 8.11e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 117 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGACTCAGAAGCTAGGTTT+AGG 0.280881 1.4:+12781209 None:intergenic
TTGCGGCCAGACTTTGTGTT+TGG 0.302196 1.4:+12781378 None:intergenic
TTTCAGTCCAGGCAAGTCTT+TGG 0.330964 1.4:-12780252 MS.gene32415:CDS
GTAGCAGTTGGTTTGAGTTC+AGG 0.333336 1.4:+12780572 None:intergenic
ATCCTTGACCACTGCTTGAT+TGG 0.334826 1.4:-12781175 MS.gene32415:CDS
GTCAAGGATGGTGTTTGTTT+CGG 0.336720 1.4:+12781189 None:intergenic
CCTCTTCCTCTTCTTAAATT+CGG 0.338434 1.4:+12780163 None:intergenic
CCTTCACACCTCAATATTGC+AGG 0.340193 1.4:-12781259 MS.gene32415:CDS
TGCAAATAATAAGTGGCTTC+AGG 0.341985 1.4:-12780534 MS.gene32415:CDS
TTAGGTTGATTGCAGCAAAA+AGG 0.349461 1.4:+12780878 None:intergenic
ATTTGAATCATATGGACATC+TGG 0.352767 1.4:-12780919 MS.gene32415:CDS
ACATAGAAGCATGAGAATTC+TGG 0.364019 1.4:+12780486 None:intergenic
AACATGGCCAAAGCGAAATA+TGG 0.367503 1.4:-12780344 MS.gene32415:CDS
ACTAGCTGACCATGAGATTC+AGG 0.371614 1.4:-12781146 MS.gene32415:CDS
TGCGGCCAGACTTTGTGTTT+GGG 0.372094 1.4:+12781379 None:intergenic
CCTAATGTTGGTCAGGGTAT+AGG 0.372172 1.4:-12780860 MS.gene32415:intron
CCAGGCAAGTCTTTGGTGCT+TGG 0.372745 1.4:-12780245 MS.gene32415:CDS
GCAGCTATAGTCTGAACAAC+AGG 0.376172 1.4:+12780218 None:intergenic
GACATCTGGGTCCAGGTTCA+TGG 0.377490 1.4:-12780905 MS.gene32415:CDS
AGCCATACGCTCGCTGAAAT+AGG 0.391985 1.4:+12781120 None:intergenic
CAATCAACCTAATGTTGGTC+AGG 0.393128 1.4:-12780867 MS.gene32415:CDS
AGAGACATCTTGGGCGTCTT+CGG 0.400681 1.4:+12780190 None:intergenic
CTCTGTCGATTTGAATCATA+TGG 0.401437 1.4:-12780927 MS.gene32415:CDS
GGACTCAGAAGCTAGGTTTA+GGG 0.401526 1.4:+12781210 None:intergenic
ATTCGGAACCAGAGACATCT+TGG 0.409795 1.4:+12780180 None:intergenic
CCTATACCCTGACCAACATT+AGG 0.410363 1.4:+12780860 None:intergenic
AACAGCACCATATTTCGCTT+TGG 0.415579 1.4:+12780337 None:intergenic
ATGGCTCGTGCTTGGAAGCC+AGG 0.426942 1.4:-12781103 MS.gene32415:CDS
AGAGTCTCAACAGTTGCATT+AGG 0.435692 1.4:+12779334 None:intergenic
CTGATGATGGAGCTCGCAAT+TGG 0.439128 1.4:-12780598 MS.gene32415:CDS
ACGATCGAGACACGCTTGCC+TGG 0.453258 1.4:+12781085 None:intergenic
TTCTGGCGTGGCACAACAGA+TGG 0.454659 1.4:+12780503 None:intergenic
GCTTTCCCAAACACAAAGTC+TGG 0.459440 1.4:-12781384 MS.gene32415:CDS
TGATGATGGAGCTCGCAATT+GGG 0.469639 1.4:-12780597 MS.gene32415:CDS
GTGGCACAACAGATGGGATT+GGG 0.470606 1.4:+12780510 None:intergenic
TTGCTGCTGATGGGGTTACA+TGG 0.473226 1.4:-12780106 MS.gene32415:CDS
CGTGGCACAACAGATGGGAT+TGG 0.478185 1.4:+12780509 None:intergenic
CTACACCATTTGCTGCTGAT+GGG 0.480152 1.4:-12780115 MS.gene32415:CDS
TCGGCATCTGCTTTCAGTCC+AGG 0.481797 1.4:-12780263 MS.gene32415:CDS
ATCAGTTAGGACAAGGATGC+AGG 0.483351 1.4:+12781291 None:intergenic
CCAAGCACCAAAGACTTGCC+TGG 0.483808 1.4:+12780245 None:intergenic
TGATTCAAATCGACAGAGGT+TGG 0.487999 1.4:+12780932 None:intergenic
TGCCTATTTCAGCGAGCGTA+TGG 0.489265 1.4:-12781122 MS.gene32415:CDS
AAGGATCCATGGAATCAGTT+AGG 0.492101 1.4:+12781278 None:intergenic
TAAGAAGAGGAAGAGGCTGC+TGG 0.495148 1.4:-12780156 MS.gene32415:CDS
TGCTGCTTCCACTAGTGTCT+CGG 0.496213 1.4:-12780282 MS.gene32415:CDS
GTGTTCTTGACGATGGTCCA+TGG 0.498059 1.4:-12781006 MS.gene32415:CDS
GAAGACGCCCAAGATGTCTC+TGG 0.498329 1.4:-12780188 MS.gene32415:CDS
GCTACACCATTTGCTGCTGA+TGG 0.499742 1.4:-12780116 MS.gene32415:CDS
GCGAGCGTATGGCTCGTGCT+TGG 0.503834 1.4:-12781111 MS.gene32415:CDS
GATGATGGAGCTCGCAATTG+GGG 0.503846 1.4:-12780596 MS.gene32415:CDS
GATTCAAATCGACAGAGGTT+GGG 0.504133 1.4:+12780933 None:intergenic
TTCGAGTTGGATGCTGATGA+TGG 0.504794 1.4:-12780611 MS.gene32415:CDS
GATGCAGGAGTGAAATTGAC+TGG 0.511013 1.4:+12781306 None:intergenic
CCTTGTCCTAACTGATTCCA+TGG 0.513859 1.4:-12781284 MS.gene32415:CDS
TGCTGCAATCAACCTAATGT+TGG 0.514462 1.4:-12780872 MS.gene32415:CDS
GTTTCAATTCCATCACAAGA+TGG 0.517536 1.4:-12781038 MS.gene32415:CDS
CACCACCTGCAGGAAAGGTT+CGG 0.519735 1.4:+12780453 None:intergenic
AGGTTTAACCATTGCTGAAG+AGG 0.530085 1.4:-12781239 MS.gene32415:CDS
GTTCTACTGCAAATAATAAG+TGG 0.530117 1.4:-12780541 MS.gene32415:CDS
TGTTGAGACTCTTGTTGCTC+CGG 0.533871 1.4:-12779323 MS.gene32415:CDS
ATGCCCAGAATTGTTCGAGT+TGG 0.537003 1.4:-12780624 MS.gene32415:intron
TGTAACACCACCTGCAGGAA+AGG 0.538038 1.4:+12780448 None:intergenic
TCATATGGACATCTGGGTCC+AGG 0.538871 1.4:-12780912 MS.gene32415:CDS
GTAACCCCATCAGCAGCAAA+TGG 0.545273 1.4:+12780110 None:intergenic
AAAGCGAAATATGGTGCTGT+TGG 0.546762 1.4:-12780335 MS.gene32415:CDS
GCTGACCGAACCTTTCCTGC+AGG 0.548268 1.4:-12780458 MS.gene32415:CDS
TTGTTTCGGACTCAGAAGCT+AGG 0.549954 1.4:+12781203 None:intergenic
ATTGAGGTGTGAAGGATCCA+TGG 0.550039 1.4:+12781267 None:intergenic
GGTTTAACCATTGCTGAAGA+GGG 0.551599 1.4:-12781238 MS.gene32415:CDS
TTTGAATCATATGGACATCT+GGG 0.553836 1.4:-12780918 MS.gene32415:CDS
GGACAACACCAGGTTGGATA+CGG 0.554055 1.4:+12780954 None:intergenic
TGAAAATTGTCATAAAGCCA+TGG 0.556477 1.4:+12780989 None:intergenic
GACTGTCAACAGCAAGCATA+CGG 0.558964 1.4:+12780405 None:intergenic
TTCGGAACCAGAGACATCTT+GGG 0.559015 1.4:+12780181 None:intergenic
TACACCATTTGCTGCTGATG+GGG 0.562334 1.4:-12780114 MS.gene32415:CDS
ACATCGGTATAAATAGCAAA+CGG 0.562622 1.4:+12781427 None:intergenic
ACAGAGGTTGGGACAACACC+AGG 0.564904 1.4:+12780944 None:intergenic
GTCATGGACCGTATCCAACC+TGG 0.567873 1.4:-12780962 MS.gene32415:CDS
TGGTTAAACCTGCAATATTG+AGG 0.568962 1.4:+12781251 None:intergenic
CCTGCAATATTGAGGTGTGA+AGG 0.569293 1.4:+12781259 None:intergenic
TCTCCTTGCTGCTCAGAACA+TGG 0.569836 1.4:-12780360 MS.gene32415:CDS
CCGAATTTAAGAAGAGGAAG+AGG 0.576312 1.4:-12780163 MS.gene32415:CDS
GTCTCGATCGTCAAAACTGA+TGG 0.576823 1.4:-12781073 MS.gene32415:CDS
GACTCAAAACTGTGTTCTTG+CGG 0.577373 1.4:+12781361 None:intergenic
GGTTAAGCCCTCTTCAGCAA+TGG 0.579403 1.4:+12781231 None:intergenic
AATAGGCAGCCTGAATCTCA+TGG 0.579809 1.4:+12781137 None:intergenic
GAACCTATGTGAGTAGCAGT+TGG 0.579851 1.4:+12780560 None:intergenic
CAATAAAATAGGCTAATGAT+CGG 0.580094 1.4:+12779297 None:intergenic
AAACCAACTGCTACTCACAT+AGG 0.586562 1.4:-12780563 MS.gene32415:CDS
CCATGGAATCAGTTAGGACA+AGG 0.596804 1.4:+12781284 None:intergenic
CTGGTTCCGAATTTAAGAAG+AGG 0.597278 1.4:-12780169 MS.gene32415:CDS
GACCAATCAAGCAGTGGTCA+AGG 0.598354 1.4:+12781173 None:intergenic
AATCAAGCAGTGGTCAAGGA+TGG 0.601061 1.4:+12781177 None:intergenic
AGGCTGCTGGAACTTCAGAA+TGG 0.602395 1.4:-12780143 MS.gene32415:CDS
ATGATGGAGCTCGCAATTGG+GGG 0.603091 1.4:-12780595 MS.gene32415:CDS
GCACTTGTAACACCACCTGC+AGG 0.604124 1.4:+12780443 None:intergenic
TCTGGCGTGGCACAACAGAT+GGG 0.604472 1.4:+12780504 None:intergenic
GAGACACTAGTGGAAGCAGC+AGG 0.614674 1.4:+12780284 None:intergenic
ATGGACCATCGTCAAGAACA+CGG 0.616907 1.4:+12781008 None:intergenic
GGGCAGCATCCATCTTGTGA+TGG 0.618902 1.4:+12781029 None:intergenic
AGCAGATGCCGAGACACTAG+TGG 0.619083 1.4:+12780274 None:intergenic
GGAAAGCATGAAGCAGACTG+TGG 0.623035 1.4:+12781400 None:intergenic
AATCAACCTAATGTTGGTCA+GGG 0.626589 1.4:-12780866 MS.gene32415:CDS
GACCGAACCTTTCCTGCAGG+TGG 0.630768 1.4:-12780455 MS.gene32415:CDS
AAAATTGCATTGGAGAGGAG+CGG 0.632329 1.4:+12780309 None:intergenic
GCTGCCCGTGTTCTTGACGA+TGG 0.635003 1.4:-12781013 MS.gene32415:CDS
TGGACCATCGTCAAGAACAC+GGG 0.639508 1.4:+12781009 None:intergenic
TGGCCATGTTCTGAGCAGCA+AGG 0.652415 1.4:+12780357 None:intergenic
AGGTTGGGACAACACCAGGT+TGG 0.657973 1.4:+12780948 None:intergenic
CATACGGTCATTAAAAGTCA+CGG 0.662964 1.4:+12780421 None:intergenic
AGCAGACTGTGGATTGACAT+CGG 0.670077 1.4:+12781411 None:intergenic
CAGCTATAGTCTGAACAACA+GGG 0.675392 1.4:+12780219 None:intergenic
ATAGGCTAATGATCGGACGC+CGG 0.675898 1.4:+12779304 None:intergenic
GAAGCATGAGAATTCTGGCG+TGG 0.677043 1.4:+12780491 None:intergenic
CATATGATTCAAATCGACAG+AGG 0.695933 1.4:+12780928 None:intergenic
GTACACGACCAATCAAGCAG+TGG 0.718956 1.4:+12781167 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTAAATATGAGAAACT+TGG - chr1.4:12780050-12780069 MS.gene32415:intron 15.0%
! AGGAACTAAATTAAATCAAG+AGG + chr1.4:12781391-12781410 None:intergenic 25.0%
!! GGAGTTCTTCAATTTTATTA+CGG + chr1.4:12780903-12780922 None:intergenic 25.0%
!! GTTACATGGAATTTTGTAAT+AGG - chr1.4:12780650-12780669 MS.gene32415:intron 25.0%
AATCCATTAATAAGCCATTG+TGG + chr1.4:12781243-12781262 None:intergenic 30.0%
AATCGTAACCAAGCAAAAAA+CGG + chr1.4:12780924-12780943 None:intergenic 30.0%
AATTGTGTTACGTTCATCAT+AGG + chr1.4:12779931-12779950 None:intergenic 30.0%
ACATCAGTTTCAACAAACAA+TGG + chr1.4:12780725-12780744 None:intergenic 30.0%
ACATCGGTATAAATAGCAAA+CGG + chr1.4:12779318-12779337 None:intergenic 30.0%
ATTTGAATCATATGGACATC+TGG - chr1.4:12779823-12779842 MS.gene32415:intron 30.0%
GTTCTACTGCAAATAATAAG+TGG - chr1.4:12780201-12780220 MS.gene32415:CDS 30.0%
TCACTTAGGAATAAAAGATC+TGG + chr1.4:12780868-12780887 None:intergenic 30.0%
TCATGTTACTATAGAGATTG+TGG - chr1.4:12781038-12781057 MS.gene32415:CDS 30.0%
TGAAAATTGTCATAAAGCCA+TGG + chr1.4:12779756-12779775 None:intergenic 30.0%
TTTGAATCATATGGACATCT+GGG - chr1.4:12779824-12779843 MS.gene32415:intron 30.0%
! TGACAATTTTCACATTGTCA+TGG - chr1.4:12779764-12779783 MS.gene32415:intron 30.0%
!! AGCAAGGAGATCATTTTTAA+GGG + chr1.4:12780372-12780391 None:intergenic 30.0%
!! TATTAATGGATTTCGTCAAG+CGG - chr1.4:12781251-12781270 MS.gene32415:CDS 30.0%
!!! AACTTGGAGTTTTTTGTTAC+AGG - chr1.4:12780066-12780085 MS.gene32415:intron 30.0%
AATAGGTCCAAAAGTTAGTC+CGG - chr1.4:12780667-12780686 MS.gene32415:intron 35.0%
AATCAACCTAATGTTGGTCA+GGG - chr1.4:12779876-12779895 MS.gene32415:intron 35.0%
AGACCACAATGGCTTATTAA+TGG - chr1.4:12781237-12781256 MS.gene32415:CDS 35.0%
AGGTAAAAATTGCATTGGAG+AGG + chr1.4:12780441-12780460 None:intergenic 35.0%
CAGGGTATAGGTAGTTATTT+AGG - chr1.4:12779894-12779913 MS.gene32415:intron 35.0%
CATACGGTCATTAAAAGTCA+CGG + chr1.4:12780324-12780343 None:intergenic 35.0%
CATATGATTCAAATCGACAG+AGG + chr1.4:12779817-12779836 None:intergenic 35.0%
CCTCTTCCTCTTCTTAAATT+CGG + chr1.4:12780582-12780601 None:intergenic 35.0%
GTTGAAACTGATGTAATGAC+AGG - chr1.4:12780731-12780750 MS.gene32415:intron 35.0%
GTTTCAATTCCATCACAAGA+TGG - chr1.4:12779704-12779723 MS.gene32415:intron 35.0%
TGCAAATAATAAGTGGCTTC+AGG - chr1.4:12780208-12780227 MS.gene32415:CDS 35.0%
TGGAGAATGACAGGTTATTA+TGG - chr1.4:12781078-12781097 MS.gene32415:CDS 35.0%
TGGTTAAACCTGCAATATTG+AGG + chr1.4:12779494-12779513 None:intergenic 35.0%
! ACATAGAAGCATGAGAATTC+TGG + chr1.4:12780259-12780278 None:intergenic 35.0%
! TACGTTCATCATAGGTTTTG+AGG + chr1.4:12779923-12779942 None:intergenic 35.0%
! TTAAGGTCTGGAATTGTACT+CGG + chr1.4:12780823-12780842 None:intergenic 35.0%
!! CAGCAAGGAGATCATTTTTA+AGG + chr1.4:12780373-12780392 None:intergenic 35.0%
!! CTCTGTCGATTTGAATCATA+TGG - chr1.4:12779815-12779834 MS.gene32415:intron 35.0%
!! TTAGGTTGATTGCAGCAAAA+AGG + chr1.4:12779867-12779886 None:intergenic 35.0%
!!! ACTTTCCACTCTTTTTTCAG+TGG + chr1.4:12779999-12780018 None:intergenic 35.0%
!!! CTTTCCACTCTTTTTTCAGT+GGG + chr1.4:12779998-12780017 None:intergenic 35.0%
AAAAAGGAAGACCATGAACC+TGG + chr1.4:12779851-12779870 None:intergenic 40.0%
AAAATTGCATTGGAGAGGAG+CGG + chr1.4:12780436-12780455 None:intergenic 40.0%
AAACCAACTGCTACTCACAT+AGG - chr1.4:12780179-12780198 MS.gene32415:CDS 40.0%
AAAGATCTGGATTGAAGTGC+CGG + chr1.4:12780855-12780874 None:intergenic 40.0%
AAATCGCCAAAAATGCACCA+CGG + chr1.4:12781174-12781193 None:intergenic 40.0%
AACAGCACCATATTTCGCTT+TGG + chr1.4:12780408-12780427 None:intergenic 40.0%
AACATGGCCAAAGCGAAATA+TGG - chr1.4:12780398-12780417 MS.gene32415:CDS 40.0%
AAGACTCTTGAACCAAGTGA+AGG + chr1.4:12781319-12781338 None:intergenic 40.0%
AAGGATCCATGGAATCAGTT+AGG + chr1.4:12779467-12779486 None:intergenic 40.0%
ACGGACTTTCAATCTCATGT+AGG + chr1.4:12779961-12779980 None:intergenic 40.0%
AGACTCTTGAACCAAGTGAA+GGG + chr1.4:12781318-12781337 None:intergenic 40.0%
AGAGTCTCAACAGTTGCATT+AGG + chr1.4:12781411-12781430 None:intergenic 40.0%
AGGTTTAACCATTGCTGAAG+AGG - chr1.4:12779503-12779522 MS.gene32415:intron 40.0%
ATAGGTCCAAAAGTTAGTCC+GGG - chr1.4:12780668-12780687 MS.gene32415:intron 40.0%
CAATCAACCTAATGTTGGTC+AGG - chr1.4:12779875-12779894 MS.gene32415:intron 40.0%
CAGCTATAGTCTGAACAACA+GGG + chr1.4:12780526-12780545 None:intergenic 40.0%
CATCCAACTCGAACAATTCT+GGG + chr1.4:12780124-12780143 None:intergenic 40.0%
CCGAATTTAAGAAGAGGAAG+AGG - chr1.4:12780579-12780598 MS.gene32415:CDS 40.0%
CTAAACTCATTTACCTAGGC+AGG + chr1.4:12780771-12780790 None:intergenic 40.0%
CTGGTTCCGAATTTAAGAAG+AGG - chr1.4:12780573-12780592 MS.gene32415:CDS 40.0%
GACTCAAAACTGTGTTCTTG+CGG + chr1.4:12779384-12779403 None:intergenic 40.0%
GAGATTGTGGATTCTGTTCA+TGG - chr1.4:12781051-12781070 MS.gene32415:CDS 40.0%
GATTCAAATCGACAGAGGTT+GGG + chr1.4:12779812-12779831 None:intergenic 40.0%
GCAGCAGGTAAAAATTGCAT+TGG + chr1.4:12780446-12780465 None:intergenic 40.0%
GCCACTAAACTCATTTACCT+AGG + chr1.4:12780775-12780794 None:intergenic 40.0%
GCCTAGGTAAATGAGTTTAG+TGG - chr1.4:12780771-12780790 MS.gene32415:intron 40.0%
GGTAAATGAGTTTAGTGGCA+TGG - chr1.4:12780776-12780795 MS.gene32415:intron 40.0%
GGTTTAACCATTGCTGAAGA+GGG - chr1.4:12779504-12779523 MS.gene32415:intron 40.0%
GTCAAGGATGGTGTTTGTTT+CGG + chr1.4:12779556-12779575 None:intergenic 40.0%
GTGGGACAGTGACATTAATA+CGG + chr1.4:12779980-12779999 None:intergenic 40.0%
GTTTAGTGGCATGGAATTGT+CGG - chr1.4:12780785-12780804 MS.gene32415:intron 40.0%
TGATTCAAATCGACAGAGGT+TGG + chr1.4:12779813-12779832 None:intergenic 40.0%
TGCTGCAATCAACCTAATGT+TGG - chr1.4:12779870-12779889 MS.gene32415:intron 40.0%
TGGATTCTGTTCATGGTACT+TGG - chr1.4:12781058-12781077 MS.gene32415:CDS 40.0%
TTCACTTGGTTCAAGAGTCT+TGG - chr1.4:12781318-12781337 MS.gene32415:CDS 40.0%
TTGTCAGATGTTCCGATAGA+AGG - chr1.4:12781288-12781307 MS.gene32415:CDS 40.0%
TTTAGTGGCATGGAATTGTC+GGG - chr1.4:12780786-12780805 MS.gene32415:intron 40.0%
! TAAGGTCTGGAATTGTACTC+GGG + chr1.4:12780822-12780841 None:intergenic 40.0%
! TCGTCAAGCGGTTATTGATT+CGG - chr1.4:12781263-12781282 MS.gene32415:CDS 40.0%
!! AAAGCGAAATATGGTGCTGT+TGG - chr1.4:12780407-12780426 MS.gene32415:CDS 40.0%
!! CATATTCACCAGTACTGACA+CGG + chr1.4:12780022-12780041 None:intergenic 40.0%
!!! GAAGAACTCCGTTTTTTGCT+TGG - chr1.4:12780913-12780932 MS.gene32415:CDS 40.0%
!!! GGAGTTTTTTGTTACAGGTG+TGG - chr1.4:12780071-12780090 MS.gene32415:intron 40.0%
AACTCTCTCATCAATTCGCG+GGG - chr1.4:12781148-12781167 MS.gene32415:CDS 45.0%
AATAGGCAGCCTGAATCTCA+TGG + chr1.4:12779608-12779627 None:intergenic 45.0%
AATCAAGCAGTGGTCAAGGA+TGG + chr1.4:12779568-12779587 None:intergenic 45.0%
ACTAGCTGACCATGAGATTC+AGG - chr1.4:12779596-12779615 MS.gene32415:intron 45.0%
ATCAGTTAGGACAAGGATGC+AGG + chr1.4:12779454-12779473 None:intergenic 45.0%
ATCCTTGACCACTGCTTGAT+TGG - chr1.4:12779567-12779586 MS.gene32415:intron 45.0%
ATGCCCAGAATTGTTCGAGT+TGG - chr1.4:12780118-12780137 MS.gene32415:CDS 45.0%
ATGGACCATCGTCAAGAACA+CGG + chr1.4:12779737-12779756 None:intergenic 45.0%
ATTCGGAACCAGAGACATCT+TGG + chr1.4:12780565-12780584 None:intergenic 45.0%
ATTGAGGTGTGAAGGATCCA+TGG + chr1.4:12779478-12779497 None:intergenic 45.0%
CAACTCTCTCATCAATTCGC+GGG - chr1.4:12781147-12781166 MS.gene32415:CDS 45.0%
CAGACCTTAAGTACCTAGTC+CGG - chr1.4:12780833-12780852 MS.gene32415:intron 45.0%
CATGGTACTTGGAGAATGAC+AGG - chr1.4:12781069-12781088 MS.gene32415:CDS 45.0%
CCAACTCTCTCATCAATTCG+CGG - chr1.4:12781146-12781165 MS.gene32415:CDS 45.0%
CCATGGAATCAGTTAGGACA+AGG + chr1.4:12779461-12779480 None:intergenic 45.0%
CCTAATGTTGGTCAGGGTAT+AGG - chr1.4:12779882-12779901 MS.gene32415:intron 45.0%
CCTATACCCTGACCAACATT+AGG + chr1.4:12779885-12779904 None:intergenic 45.0%
CCTGCAATATTGAGGTGTGA+AGG + chr1.4:12779486-12779505 None:intergenic 45.0%
CCTTCACACCTCAATATTGC+AGG - chr1.4:12779483-12779502 MS.gene32415:intron 45.0%
CCTTGATGCTAGTGAGAAGA+GGG - chr1.4:12781203-12781222 MS.gene32415:CDS 45.0%
CTCTACCAATGCGGTCTATA+GGG + chr1.4:12780954-12780973 None:intergenic 45.0%
CTGTCCCACTGAAAAAAGAG+TGG - chr1.4:12779991-12780010 MS.gene32415:intron 45.0%
CTTGATGCTAGTGAGAAGAG+GGG - chr1.4:12781204-12781223 MS.gene32415:CDS 45.0%
GAACCTATGTGAGTAGCAGT+TGG + chr1.4:12780185-12780204 None:intergenic 45.0%
GACTGTCAACAGCAAGCATA+CGG + chr1.4:12780340-12780359 None:intergenic 45.0%
GAGTTGGCGAAGAAAATTCC+AGG + chr1.4:12781133-12781152 None:intergenic 45.0%
GATGCAGGAGTGAAATTGAC+TGG + chr1.4:12779439-12779458 None:intergenic 45.0%
GCAGCTATAGTCTGAACAAC+AGG + chr1.4:12780527-12780546 None:intergenic 45.0%
GCATCCAACTCGAACAATTC+TGG + chr1.4:12780125-12780144 None:intergenic 45.0%
GCTTTCCCAAACACAAAGTC+TGG - chr1.4:12779358-12779377 MS.gene32415:intron 45.0%
GGACTCAGAAGCTAGGTTTA+GGG + chr1.4:12779535-12779554 None:intergenic 45.0%
GTCGCAATTCTAGACCACAA+TGG - chr1.4:12781226-12781245 MS.gene32415:CDS 45.0%
GTCTCGATCGTCAAAACTGA+TGG - chr1.4:12779669-12779688 MS.gene32415:intron 45.0%
GTGAAGGGATAGCCTTCTAT+CGG + chr1.4:12781303-12781322 None:intergenic 45.0%
TAGAAGGCTATCCCTTCACT+TGG - chr1.4:12781304-12781323 MS.gene32415:CDS 45.0%
TAGGTCCAAAAGTTAGTCCG+GGG - chr1.4:12780669-12780688 MS.gene32415:intron 45.0%
TCCTTGATGCTAGTGAGAAG+AGG - chr1.4:12781202-12781221 MS.gene32415:CDS 45.0%
TGTTGAGACTCTTGTTGCTC+CGG - chr1.4:12781419-12781438 MS.gene32415:CDS 45.0%
TTAGTGGCATGGAATTGTCG+GGG - chr1.4:12780787-12780806 MS.gene32415:intron 45.0%
TTCATATCTGTATCACCCCC+CGG + chr1.4:12780689-12780708 None:intergenic 45.0%
TTCGGAACCAGAGACATCTT+GGG + chr1.4:12780564-12780583 None:intergenic 45.0%
TTGTTTCGGACTCAGAAGCT+AGG + chr1.4:12779542-12779561 None:intergenic 45.0%
TTTCAGTCCAGGCAAGTCTT+TGG - chr1.4:12780490-12780509 MS.gene32415:CDS 45.0%
! AGCAGACTGTGGATTGACAT+CGG + chr1.4:12779334-12779353 None:intergenic 45.0%
! CCTTGTCCTAACTGATTCCA+TGG - chr1.4:12779458-12779477 MS.gene32415:intron 45.0%
! CTACACCATTTGCTGCTGAT+GGG - chr1.4:12780627-12780646 MS.gene32415:intron 45.0%
! TACACCATTTGCTGCTGATG+GGG - chr1.4:12780628-12780647 MS.gene32415:intron 45.0%
!! AATGACAGGCACTGATGATC+AGG - chr1.4:12780745-12780764 MS.gene32415:intron 45.0%
!! CGTCAAGCGGTTATTGATTC+GGG - chr1.4:12781264-12781283 MS.gene32415:CDS 45.0%
!! GTAGCAGTTGGTTTGAGTTC+AGG + chr1.4:12780173-12780192 None:intergenic 45.0%
!! TGATGATGGAGCTCGCAATT+GGG - chr1.4:12780145-12780164 MS.gene32415:CDS 45.0%
!! TTCGAGTTGGATGCTGATGA+TGG - chr1.4:12780131-12780150 MS.gene32415:CDS 45.0%
AGAGACATCTTGGGCGTCTT+CGG + chr1.4:12780555-12780574 None:intergenic 50.0%
AGCCATACGCTCGCTGAAAT+AGG + chr1.4:12779625-12779644 None:intergenic 50.0%
AGGCTGCTGGAACTTCAGAA+TGG - chr1.4:12780599-12780618 MS.gene32415:CDS 50.0%
AGGTCCAAAAGTTAGTCCGG+GGG - chr1.4:12780670-12780689 MS.gene32415:intron 50.0%
AGGTTATTATGGCTACCCCG+AGG - chr1.4:12781089-12781108 MS.gene32415:CDS 50.0%
CCCTCTTCTCACTAGCATCA+AGG + chr1.4:12781206-12781225 None:intergenic 50.0%
CCGCGAATTGATGAGAGAGT+TGG + chr1.4:12781149-12781168 None:intergenic 50.0%
CCTCTACCAATGCGGTCTAT+AGG + chr1.4:12780955-12780974 None:intergenic 50.0%
CGGACTCAGAAGCTAGGTTT+AGG + chr1.4:12779536-12779555 None:intergenic 50.0%
GAAGCATGAGAATTCTGGCG+TGG + chr1.4:12780254-12780273 None:intergenic 50.0%
GACCAATCAAGCAGTGGTCA+AGG + chr1.4:12779572-12779591 None:intergenic 50.0%
GAGAGGTTAGATCGTGCTCT+TGG - chr1.4:12781360-12781379 MS.gene32415:CDS 50.0%
GCATGGAATTGTCGGGGTTT+AGG - chr1.4:12780793-12780812 MS.gene32415:intron 50.0%
GGTGAGCAAGAGTCTCACTT+AGG + chr1.4:12780882-12780901 None:intergenic 50.0%
GGTTAAGCCCTCTTCAGCAA+TGG + chr1.4:12779514-12779533 None:intergenic 50.0%
GGTTATTATGGCTACCCCGA+GGG - chr1.4:12781090-12781109 MS.gene32415:CDS 50.0%
GTAACCCCATCAGCAGCAAA+TGG + chr1.4:12780635-12780654 None:intergenic 50.0%
GTACACGACCAATCAAGCAG+TGG + chr1.4:12779578-12779597 None:intergenic 50.0%
GTGGCACAACAGATGGGATT+GGG + chr1.4:12780235-12780254 None:intergenic 50.0%
GTGTTCTTGACGATGGTCCA+TGG - chr1.4:12779736-12779755 MS.gene32415:intron 50.0%
TAAGAAGAGGAAGAGGCTGC+TGG - chr1.4:12780586-12780605 MS.gene32415:CDS 50.0%
TCATATGGACATCTGGGTCC+AGG - chr1.4:12779830-12779849 MS.gene32415:intron 50.0%
TCTCCTTGCTGCTCAGAACA+TGG - chr1.4:12780382-12780401 MS.gene32415:CDS 50.0%
TGCCTATTTCAGCGAGCGTA+TGG - chr1.4:12779620-12779639 MS.gene32415:intron 50.0%
TGCGGCCAGACTTTGTGTTT+GGG + chr1.4:12779366-12779385 None:intergenic 50.0%
TGGAAAGTCCGTGTCAGTAC+TGG - chr1.4:12780011-12780030 MS.gene32415:intron 50.0%
TGGACCATCGTCAAGAACAC+GGG + chr1.4:12779736-12779755 None:intergenic 50.0%
TGTAACACCACCTGCAGGAA+AGG + chr1.4:12780297-12780316 None:intergenic 50.0%
TGTCTCCCTATAGACCGCAT+TGG - chr1.4:12780946-12780965 MS.gene32415:CDS 50.0%
TTGCGGCCAGACTTTGTGTT+TGG + chr1.4:12779367-12779386 None:intergenic 50.0%
TTGCTGCTGATGGGGTTACA+TGG - chr1.4:12780636-12780655 MS.gene32415:intron 50.0%
! AGTGCCGGACTAGGTACTTA+AGG + chr1.4:12780840-12780859 None:intergenic 50.0%
! ATAGACCGCATTGGTAGAGG+CGG - chr1.4:12780955-12780974 MS.gene32415:CDS 50.0%
! ATGATGGAGCTCGCAATTGG+GGG - chr1.4:12780147-12780166 MS.gene32415:CDS 50.0%
! CCTATAGACCGCATTGGTAG+AGG - chr1.4:12780952-12780971 MS.gene32415:CDS 50.0%
! CGGACTAGGTACTTAAGGTC+TGG + chr1.4:12780835-12780854 None:intergenic 50.0%
! GCTACACCATTTGCTGCTGA+TGG - chr1.4:12780626-12780645 MS.gene32415:intron 50.0%
! GGAAAGCATGAAGCAGACTG+TGG + chr1.4:12779345-12779364 None:intergenic 50.0%
! GGACAACACCAGGTTGGATA+CGG + chr1.4:12779791-12779810 None:intergenic 50.0%
! TGCTGCTTCCACTAGTGTCT+CGG - chr1.4:12780460-12780479 MS.gene32415:CDS 50.0%
! TGTTACAGGTGTGGACTGCT+TGG - chr1.4:12780080-12780099 MS.gene32415:intron 50.0%
!! CTGATGATGGAGCTCGCAAT+TGG - chr1.4:12780144-12780163 MS.gene32415:CDS 50.0%
!! GATGATGGAGCTCGCAATTG+GGG - chr1.4:12780146-12780165 MS.gene32415:CDS 50.0%
!! GCGGGGGTCTAGCTTTAATT+TGG - chr1.4:12780974-12780993 MS.gene32415:CDS 50.0%
ACAGAGGTTGGGACAACACC+AGG + chr1.4:12779801-12779820 None:intergenic 55.0%
ACTGATGATCAGGCCTGCCT+AGG - chr1.4:12780755-12780774 MS.gene32415:intron 55.0%
AGCAGATGCCGAGACACTAG+TGG + chr1.4:12780471-12780490 None:intergenic 55.0%
AGGTTGGGACAACACCAGGT+TGG + chr1.4:12779797-12779816 None:intergenic 55.0%
CACCACCTGCAGGAAAGGTT+CGG + chr1.4:12780292-12780311 None:intergenic 55.0%
CCAAGCACCAAAGACTTGCC+TGG + chr1.4:12780500-12780519 None:intergenic 55.0%
CGTGGCACAACAGATGGGAT+TGG + chr1.4:12780236-12780255 None:intergenic 55.0%
GAAGACGCCCAAGATGTCTC+TGG - chr1.4:12780554-12780573 MS.gene32415:CDS 55.0%
GACATCTGGGTCCAGGTTCA+TGG - chr1.4:12779837-12779856 MS.gene32415:intron 55.0%
GAGACACTAGTGGAAGCAGC+AGG + chr1.4:12780461-12780480 None:intergenic 55.0%
GCACTTGTAACACCACCTGC+AGG + chr1.4:12780302-12780321 None:intergenic 55.0%
GGGCAGCATCCATCTTGTGA+TGG + chr1.4:12779716-12779735 None:intergenic 55.0%
GGTCCAAAAGTTAGTCCGGG+GGG - chr1.4:12780671-12780690 MS.gene32415:intron 55.0%
GTCATGGACCGTATCCAACC+TGG - chr1.4:12779780-12779799 MS.gene32415:intron 55.0%
TATTATGGCTACCCCGAGGG+AGG - chr1.4:12781093-12781112 MS.gene32415:CDS 55.0%
TCGGCATCTGCTTTCAGTCC+AGG - chr1.4:12780479-12780498 MS.gene32415:CDS 55.0%
TCTGGCGTGGCACAACAGAT+GGG + chr1.4:12780241-12780260 None:intergenic 55.0%
TTCTGGCGTGGCACAACAGA+TGG + chr1.4:12780242-12780261 None:intergenic 55.0%
! TAGACCGCATTGGTAGAGGC+GGG - chr1.4:12780956-12780975 MS.gene32415:CDS 55.0%
! TCACCCCCCGGACTAACTTT+TGG + chr1.4:12780677-12780696 None:intergenic 55.0%
! TGGCCATGTTCTGAGCAGCA+AGG + chr1.4:12780388-12780407 None:intergenic 55.0%
!! CCAGGCAAGTCTTTGGTGCT+TGG - chr1.4:12780497-12780516 MS.gene32415:CDS 55.0%
!! CTGGATTGAAGTGCCGGACT+AGG + chr1.4:12780849-12780868 None:intergenic 55.0%
AACCTCTCCTCCACCGCTTG+CGG + chr1.4:12781348-12781367 None:intergenic 60.0%
ACGATCGAGACACGCTTGCC+TGG + chr1.4:12779660-12779679 None:intergenic 60.0%
ATGGCTCGTGCTTGGAAGCC+AGG - chr1.4:12779639-12779658 MS.gene32415:intron 60.0%
GACCGAACCTTTCCTGCAGG+TGG - chr1.4:12780287-12780306 MS.gene32415:CDS 60.0%
GCTGACCGAACCTTTCCTGC+AGG - chr1.4:12780284-12780303 MS.gene32415:CDS 60.0%
GCTGCCCGTGTTCTTGACGA+TGG - chr1.4:12779729-12779748 MS.gene32415:intron 60.0%
! AGACCGCATTGGTAGAGGCG+GGG - chr1.4:12780957-12780976 MS.gene32415:CDS 60.0%
AGCACGCCTACGACCTCCCT+CGG + chr1.4:12781109-12781128 None:intergenic 65.0%
CTCATCAATTCGCGGGGCCG+TGG - chr1.4:12781154-12781173 MS.gene32415:CDS 65.0%
GACCCCCGCCTCTACCAATG+CGG + chr1.4:12780963-12780982 None:intergenic 65.0%
GAGTCTTGGCACGCCGCAAG+CGG - chr1.4:12781332-12781351 MS.gene32415:CDS 65.0%
GCGAGCGTATGGCTCGTGCT+TGG - chr1.4:12779631-12779650 MS.gene32415:intron 65.0%
! GACCGCATTGGTAGAGGCGG+GGG - chr1.4:12780958-12780977 MS.gene32415:CDS 65.0%
! GCGGGGCCGTGGTGCATTTT+TGG - chr1.4:12781165-12781184 MS.gene32415:CDS 65.0%
CACGCCTACGACCTCCCTCG+GGG + chr1.4:12781107-12781126 None:intergenic 70.0%
GAGGTCGTAGGCGTGCTGCC+TGG - chr1.4:12781112-12781131 MS.gene32415:CDS 70.0%
GCACGCCTACGACCTCCCTC+GGG + chr1.4:12781108-12781127 None:intergenic 70.0%
GCTACCCCGAGGGAGGTCGT+AGG - chr1.4:12781100-12781119 MS.gene32415:CDS 70.0%
TCTTGGCACGCCGCAAGCGG+TGG - chr1.4:12781335-12781354 MS.gene32415:CDS 70.0%
CGCCGCAAGCGGTGGAGGAG+AGG - chr1.4:12781343-12781362 MS.gene32415:CDS 75.0%
TGGCACGCCGCAAGCGGTGG+AGG - chr1.4:12781338-12781357 MS.gene32415:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 12779309 12781455 12779309 ID=MS.gene32415
chr1.4 mRNA 12779309 12781455 12779309 ID=MS.gene32415.t1;Parent=MS.gene32415
chr1.4 exon 12780861 12781455 12780861 ID=MS.gene32415.t1.exon1;Parent=MS.gene32415.t1
chr1.4 CDS 12780861 12781455 12780861 ID=cds.MS.gene32415.t1;Parent=MS.gene32415.t1
chr1.4 exon 12780093 12780638 12780093 ID=MS.gene32415.t1.exon2;Parent=MS.gene32415.t1
chr1.4 CDS 12780093 12780638 12780093 ID=cds.MS.gene32415.t1;Parent=MS.gene32415.t1
chr1.4 exon 12779309 12779358 12779309 ID=MS.gene32415.t1.exon3;Parent=MS.gene32415.t1
chr1.4 CDS 12779309 12779358 12779309 ID=cds.MS.gene32415.t1;Parent=MS.gene32415.t1
Gene Sequence

>MS.gene32415

ATGCTTCCGTTTGCTATTTATACCGATGTCAATCCACAGTCTGCTTCATGCTTTCCCAAACACAAAGTCTGGCCGCAAGAACACAGTTTTGAGTCTGTTTTTGTTTCGTCGTTCTTCTCTTCTGTTTCCAGTCAATTTCACTCCTGCATCCTTGTCCTAACTGATTCCATGGATCCTTCACACCTCAATATTGCAGGTTTAACCATTGCTGAAGAGGGCTTAACCCTAAACCTAGCTTCTGAGTCCGAAACAAACACCATCCTTGACCACTGCTTGATTGGTCGTGTACTAGCTGACCATGAGATTCAGGCTGCCTATTTCAGCGAGCGTATGGCTCGTGCTTGGAAGCCAGGCAAGCGTGTCTCGATCGTCAAAACTGATGGTGATCGCTTCTTGTTTCAATTCCATCACAAGATGGATGCTGCCCGTGTTCTTGACGATGGTCCATGGCTTTATGACAATTTTCACATTGTCATGGACCGTATCCAACCTGGTGTTGTCCCAACCTCTGTCGATTTGAATCATATGGACATCTGGGTCCAGGTTCATGGTCTTCCTTTTTGCTGCAATCAACCTAATGTTGGTCAGGGTATAGGTAGTTATTTAGGTGTCCTCAAAACCTATGATGAACGTAACACAATTCATAGTACCTACATGAGATTGAAAGTCCGTATTAATGTCACTGTCCCACTGAAAAAAGAGTGGAAAGTCCGTGTCAGTACTGGTGAATATGTTACTGTTAAATTTAAATATGAGAAACTTGGAGTTTTTTGTTACAGGTGTGGACTGCTTGGACACACAGACAAAGTATGCCCAGAATTGTTCGAGTTGGATGCTGATGATGGAGCTCGCAATTGGGGGCCTGAACTCAAACCAACTGCTACTCACATAGGTTCTACTGCAAATAATAAGTGGCTTCAGGACCCAATCCCATCTGTTGTGCCACGCCAGAATTCTCATGCTTCTATGTTTCATGCTGACCGAACCTTTCCTGCAGGTGGTGTTACAAGTGCCGTGACTTTTAATGACCGTATGCTTGCTGTTGACAGTCAAATTTCTGCCCTTAAAAATGATCTCCTTGCTGCTCAGAACATGGCCAAAGCGAAATATGGTGCTGTTGGCAACCGCTCCTCTCCAATGCAATTTTTACCTGCTGCTTCCACTAGTGTCTCGGCATCTGCTTTCAGTCCAGGCAAGTCTTTGGTGCTTGGTCTCCCTGTTGTTCAGACTATAGCTGCTGACTCCGAAGACGCCCAAGATGTCTCTGGTTCCGAATTTAAGAAGAGGAAGAGGCTGCTGGAACTTCAGAATGGTGAAGCTACACCATTTGCTGCTGATGGGGTTACATGGAATTTTGTAATAGGTCCAAAAGTTAGTCCGGGGGGTGATACAGATATGAATGTAATCTCTAATCCATTGTTTGTTGAAACTGATGTAATGACAGGCACTGATGATCAGGCCTGCCTAGGTAAATGAGTTTAGTGGCATGGAATTGTCGGGGTTTAGGTAGCCCGAGTACAATTCCAGACCTTAAGTACCTAGTCCGGCACTTCAATCCAGATCTTTTATTCCTAAGTGAGACTCTTGCTCACCGTAATAAAATTGAAGAACTCCGTTTTTTGCTTGGTTACGATTCTTGTCTCCCTATAGACCGCATTGGTAGAGGCGGGGGTCTAGCTTTAATTTGGCGCAATTCATTCAATTGTCATCTTATTTATTTTTCTAATAATCATGTTACTATAGAGATTGTGGATTCTGTTCATGGTACTTGGAGAATGACAGGTTATTATGGCTACCCCGAGGGAGGTCGTAGGCGTGCTGCCTGGAATTTTCTTCGCCAACTCTCTCATCAATTCGCGGGGCCGTGGTGCATTTTTGGCGATTTCAACGACATCCTTGATGCTAGTGAGAAGAGGGGTCGCAATTCTAGACCACAATGGCTTATTAATGGATTTCGTCAAGCGGTTATTGATTCGGGCTTGTCAGATGTTCCGATAGAAGGCTATCCCTTCACTTGGTTCAAGAGTCTTGGCACGCCGCAAGCGGTGGAGGAGAGGTTAGATCGTGCTCTTGGTAACACCTCTTGATTTAATTTAGTTCCTAATGCAACTGTTGAGACTCTTGTTGCTCCGGCGTCCGATCATTAG
Protein sequence

>MS.gene32415.t1

MLPFAIYTDVNPQSASCFPKHKVWPQEHSFESVFVSSFFSSVSSQFHSCILVLTDSMDPSHLNIAGLTIAEEGLTLNLASESETNTILDHCLIGRVLADHEIQAAYFSERMARAWKPGKRVSIVKTDGDRFLFQFHHKMDAARVLDDGPWLYDNFHIVMDRIQPGVVPTSVDLNHMDIWVQVHGLPFCCNQPNVGQGIELFELDADDGARNWGPELKPTATHIGSTANNKWLQDPIPSVVPRQNSHASMFHADRTFPAGGVTSAVTFNDRMLAVDSQISALKNDLLAAQNMAKAKYGAVGNRSSPMQFLPAASTSVSASAFSPGKSLVLGLPVVQTIAADSEDAQDVSGSEFKKRKRLLELQNGEATPFAADGVTWNFVIVPNATVETLVAPASDH