AlfalfaGEDB | MS.gene054652

M. sativa cultivar XinJiangDaYe / MS.gene054652

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054652.t1	XP_024630779.1	79.2	265	48	2	1	259	1	264	1.00E-122	449.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054652.t1	A0A396JIW9	76.1	243	58	0	1	243	1	243	1.2e-109	405.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene054652	MS.gene34463	PPI
MS.gene47792	MS.gene054652	PPI
MS.gene46948	MS.gene054652	PPI
MS.gene025873	MS.gene054652	PPI
MS.gene012019	MS.gene054652	PPI
MS.gene054652	MS.gene61499	PPI
MS.gene05358	MS.gene054652	PPI
MS.gene054652	MS.gene019165	PPI
MS.gene074257	MS.gene054652	PPI
MS.gene054652	MS.gene81194	PPI
MS.gene054652	MS.gene038394	PPI
MS.gene000629	MS.gene054652	PPI
MS.gene041264	MS.gene054652	PPI
MS.gene054652	MS.gene019001	PPI
MS.gene41979	MS.gene054652	PPI
MS.gene024522	MS.gene054652	PPI
MS.gene054652	MS.gene53904	PPI
MS.gene05319	MS.gene054652	PPI
MS.gene054652	MS.gene73261	PPI
MS.gene038098	MS.gene054652	PPI
MS.gene56991	MS.gene054652	PPI
MS.gene054652	MS.gene025675	PPI
MS.gene85538	MS.gene054652	PPI
MS.gene054652	MS.gene055546	PPI
MS.gene055223	MS.gene054652	PPI
MS.gene28545	MS.gene054652	PPI
MS.gene054652	MS.gene55209	PPI
MS.gene28718	MS.gene054652	PPI
MS.gene053747	MS.gene054652	PPI
MS.gene061850	MS.gene054652	PPI
MS.gene054652	MS.gene022929	PPI
MS.gene054652	MS.gene068629	PPI
MS.gene054652	MS.gene009781	PPI
MS.gene073582	MS.gene054652	PPI
MS.gene058889	MS.gene054652	PPI
MS.gene054652	MS.gene048342	PPI
MS.gene98616	MS.gene054652	PPI
MS.gene40828	MS.gene054652	PPI
MS.gene046089	MS.gene054652	PPI
MS.gene054652	MS.gene069624	PPI
MS.gene042027	MS.gene054652	PPI
MS.gene054652	MS.gene21066	PPI
MS.gene054652	MS.gene013890	PPI
MS.gene69490	MS.gene054652	PPI
MS.gene013644	MS.gene054652	PPI
MS.gene054652	MS.gene016719	PPI
MS.gene054652	MS.gene028714	PPI
MS.gene80362	MS.gene054652	PPI
MS.gene72520	MS.gene054652	PPI
MS.gene054652	MS.gene048706	PPI
MS.gene013193	MS.gene054652	PPI
MS.gene054652	MS.gene69586	PPI
MS.gene04970	MS.gene054652	PPI
MS.gene054652	MS.gene45083	PPI
MS.gene08106	MS.gene054652	PPI
MS.gene054652	MS.gene049561	PPI
MS.gene054652	MS.gene008658	PPI
MS.gene054652	MS.gene49057	PPI
MS.gene63743	MS.gene054652	PPI
MS.gene054652	MS.gene08708	PPI
MS.gene024405	MS.gene054652	PPI
MS.gene012748	MS.gene054652	PPI
MS.gene04134	MS.gene054652	PPI
MS.gene83979	MS.gene054652	PPI
MS.gene062067	MS.gene054652	PPI
MS.gene054652	MS.gene012889	PPI
MS.gene57044	MS.gene054652	PPI
MS.gene054652	MS.gene000506	PPI
MS.gene98471	MS.gene054652	PPI
MS.gene049610	MS.gene054652	PPI
MS.gene038676	MS.gene054652	PPI
MS.gene060718	MS.gene054652	PPI
MS.gene71637	MS.gene054652	PPI
MS.gene054652	MS.gene028716	PPI
MS.gene054652	MS.gene74639	PPI
MS.gene054652	MS.gene071133	PPI
MS.gene054652	MS.gene60266	PPI
MS.gene042651	MS.gene054652	PPI
MS.gene93396	MS.gene054652	PPI
MS.gene054652	MS.gene024522	PPI
MS.gene054652	MS.gene54051	PPI
MS.gene05576	MS.gene054652	PPI
MS.gene054652	MS.gene22111	PPI
MS.gene05231	MS.gene054652	PPI
MS.gene054652	MS.gene37955	PPI
MS.gene98467	MS.gene054652	PPI
MS.gene010656	MS.gene054652	PPI
MS.gene037049	MS.gene054652	PPI
MS.gene034643	MS.gene054652	PPI
MS.gene054652	MS.gene010890	PPI
MS.gene054652	MS.gene84790	PPI
MS.gene037634	MS.gene054652	PPI
MS.gene64130	MS.gene054652	PPI
MS.gene050679	MS.gene054652	PPI
MS.gene20246	MS.gene054652	PPI
MS.gene054652	MS.gene060788	PPI
MS.gene071784	MS.gene054652	PPI
MS.gene054652	MS.gene77990	PPI
MS.gene061579	MS.gene054652	PPI
MS.gene054652	MS.gene062511	PPI
MS.gene76420	MS.gene054652	PPI
MS.gene071320	MS.gene054652	PPI
MS.gene65471	MS.gene054652	PPI
MS.gene054652	MS.gene027994	PPI
MS.gene054652	MS.gene007022	PPI
MS.gene026680	MS.gene054652	PPI
MS.gene26433	MS.gene054652	PPI
MS.gene054652	MS.gene69584	PPI
MS.gene054652	MS.gene52349	PPI
MS.gene042559	MS.gene054652	PPI
MS.gene054652	MS.gene46353	PPI
MS.gene22200	MS.gene054652	PPI
MS.gene26038	MS.gene054652	PPI
MS.gene054652	MS.gene61651	PPI
MS.gene85308	MS.gene054652	PPI
MS.gene015248	MS.gene054652	PPI
MS.gene34812	MS.gene054652	PPI
MS.gene054652	MS.gene88393	PPI
MS.gene02896	MS.gene054652	PPI
MS.gene054652	MS.gene054508	PPI
MS.gene44083	MS.gene054652	PPI
MS.gene55682	MS.gene054652	PPI
MS.gene054652	MS.gene95960	PPI
MS.gene054652	MS.gene053373	PPI
MS.gene58419	MS.gene054652	PPI
MS.gene067340	MS.gene054652	PPI
MS.gene054652	MS.gene63150	PPI
MS.gene65623	MS.gene054652	PPI
MS.gene23521	MS.gene054652	PPI
MS.gene054652	MS.gene65474	PPI
MS.gene049512	MS.gene054652	PPI
MS.gene29406	MS.gene054652	PPI
MS.gene054652	MS.gene025401	PPI
MS.gene054652	MS.gene015093	PPI
MS.gene038623	MS.gene054652	PPI
MS.gene86275	MS.gene054652	PPI
MS.gene05357	MS.gene054652	PPI
MS.gene054652	MS.gene06164	PPI
MS.gene054652	MS.gene50392	PPI
MS.gene054652	MS.gene22730	PPI
MS.gene032311	MS.gene054652	PPI
MS.gene87529	MS.gene054652	PPI
MS.gene054652	MS.gene000379	PPI
MS.gene054652	MS.gene032299	PPI
MS.gene054652	MS.gene037634	PPI
MS.gene054652	MS.gene08606	PPI
MS.gene86849	MS.gene054652	PPI
MS.gene072852	MS.gene054652	PPI
MS.gene054652	MS.gene000640	PPI
MS.gene054652	MS.gene09517	PPI
MS.gene054652	MS.gene98756	PPI
MS.gene054652	MS.gene87845	PPI
MS.gene39956	MS.gene054652	PPI
MS.gene017586	MS.gene054652	PPI
MS.gene021968	MS.gene054652	PPI
MS.gene09589	MS.gene054652	PPI
MS.gene054652	MS.gene62249	PPI
MS.gene43393	MS.gene054652	PPI
MS.gene054652	MS.gene010290	PPI
MS.gene057828	MS.gene054652	PPI
MS.gene054652	MS.gene92517	PPI
MS.gene66650	MS.gene054652	PPI
MS.gene054652	MS.gene044729	PPI
MS.gene005120	MS.gene054652	PPI
MS.gene054652	MS.gene60390	PPI
MS.gene054652	MS.gene039692	PPI
MS.gene060386	MS.gene054652	PPI
MS.gene71588	MS.gene054652	PPI
MS.gene054652	MS.gene002590	PPI
MS.gene04749	MS.gene054652	PPI
MS.gene05436	MS.gene054652	PPI
MS.gene059736	MS.gene054652	PPI
MS.gene70932	MS.gene054652	PPI
MS.gene42903	MS.gene054652	PPI
MS.gene054652	MS.gene024297	PPI
MS.gene81712	MS.gene054652	PPI
MS.gene054652	MS.gene059269	PPI
MS.gene054652	MS.gene057934	PPI
MS.gene021965	MS.gene054652	PPI
MS.gene055683	MS.gene054652	PPI
MS.gene042614	MS.gene054652	PPI
MS.gene054652	MS.gene37537	PPI
MS.gene41643	MS.gene054652	PPI
MS.gene054652	MS.gene024841	PPI
MS.gene26844	MS.gene054652	PPI
MS.gene021555	MS.gene054652	PPI
MS.gene054652	MS.gene069786	PPI
MS.gene048747	MS.gene054652	PPI
MS.gene021647	MS.gene054652	PPI
MS.gene054652	MS.gene42010	PPI
MS.gene054652	MS.gene025158	PPI
MS.gene019165	MS.gene054652	PPI
MS.gene054652	MS.gene51799	PPI
MS.gene009121	MS.gene054652	PPI
MS.gene94654	MS.gene054652	PPI
MS.gene073573	MS.gene054652	PPI
MS.gene031967	MS.gene054652	PPI
MS.gene054652	MS.gene40469	PPI
MS.gene054652	MS.gene09023	PPI
MS.gene054652	MS.gene025987	PPI
MS.gene063214	MS.gene054652	PPI
MS.gene97410	MS.gene054652	PPI
MS.gene83772	MS.gene054652	PPI
MS.gene32415	MS.gene054652	PPI
MS.gene054652	MS.gene043140	PPI
MS.gene035626	MS.gene054652	PPI
MS.gene008927	MS.gene054652	PPI
MS.gene045614	MS.gene054652	PPI
MS.gene054652	MS.gene03149	PPI
MS.gene63762	MS.gene054652	PPI
MS.gene054652	MS.gene29321	PPI
MS.gene71675	MS.gene054652	PPI
MS.gene30208	MS.gene054652	PPI
MS.gene69495	MS.gene054652	PPI
MS.gene013676	MS.gene054652	PPI
MS.gene054652	MS.gene24278	PPI
MS.gene64178	MS.gene054652	PPI
MS.gene054652	MS.gene34618	PPI
MS.gene030090	MS.gene054652	PPI
MS.gene95561	MS.gene054652	PPI
MS.gene96174	MS.gene054652	PPI
MS.gene054652	MS.gene59460	PPI
MS.gene018196	MS.gene054652	PPI
MS.gene054652	MS.gene060773	PPI
MS.gene065627	MS.gene054652	PPI
MS.gene57019	MS.gene054652	PPI
MS.gene054652	MS.gene08713	PPI
MS.gene018745	MS.gene054652	PPI
MS.gene65546	MS.gene054652	PPI
MS.gene03936	MS.gene054652	PPI
MS.gene054652	MS.gene24070	PPI
MS.gene054652	MS.gene85538	PPI
MS.gene60059	MS.gene054652	PPI
MS.gene054652	MS.gene64178	PPI
MS.gene34197	MS.gene054652	PPI
MS.gene054652	MS.gene073573	PPI
MS.gene055560	MS.gene054652	PPI
MS.gene008275	MS.gene054652	PPI
MS.gene054652	MS.gene44144	PPI
MS.gene038757	MS.gene054652	PPI
MS.gene054652	MS.gene060718	PPI
MS.gene064966	MS.gene054652	PPI
MS.gene048072	MS.gene054652	PPI
MS.gene002590	MS.gene054652	PPI
MS.gene054652	MS.gene027169	PPI
MS.gene054652	MS.gene004417	PPI
MS.gene054652	MS.gene019177	PPI
MS.gene43924	MS.gene054652	PPI
MS.gene047954	MS.gene054652	PPI
MS.gene35175	MS.gene054652	PPI
MS.gene036423	MS.gene054652	PPI
MS.gene054652	MS.gene009213	PPI
MS.gene054652	MS.gene98127	PPI
MS.gene054652	MS.gene003264	PPI
MS.gene66651	MS.gene054652	PPI
MS.gene96109	MS.gene054652	PPI
MS.gene054652	MS.gene27329	PPI
MS.gene35202	MS.gene054652	PPI
MS.gene054652	MS.gene41393	PPI
MS.gene051449	MS.gene054652	PPI
MS.gene054652	MS.gene89815	PPI
MS.gene054652	MS.gene016632	PPI
MS.gene054652	MS.gene018474	PPI
MS.gene054652	MS.gene048088	PPI
MS.gene054652	MS.gene86868	PPI
MS.gene48477	MS.gene054652	PPI
MS.gene51804	MS.gene054652	PPI
MS.gene045875	MS.gene054652	PPI
MS.gene36069	MS.gene054652	PPI
MS.gene054652	MS.gene96163	PPI
MS.gene30136	MS.gene054652	PPI
MS.gene008861	MS.gene054652	PPI
MS.gene61452	MS.gene054652	PPI
MS.gene016102	MS.gene054652	PPI
MS.gene46941	MS.gene054652	PPI
MS.gene96541	MS.gene054652	PPI
MS.gene000634	MS.gene054652	PPI
MS.gene85295	MS.gene054652	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054652.t1	MTR_0010s0370	74.863	183	46	0	1	183	1	183	6.13e-100	290
MS.gene054652.t1	MTR_0010s0350	74.863	183	46	0	1	183	1	183	6.13e-100	290
MS.gene054652.t1	MTR_1g036720	74.775	111	28	0	1	111	1	111	1.60e-60	187
MS.gene054652.t1	MTR_1g036710	48.092	131	30	2	132	258	1	97	2.65e-31	117
MS.gene054652.t1	MTR_6g033215	31.319	182	119	3	20	199	117	294	1.40e-27	107
MS.gene054652.t1	MTR_7g089990	47.222	108	55	1	104	209	86	193	2.30e-22	92.0
MS.gene054652.t1	MTR_3g053920	34.058	138	74	2	34	171	36	156	3.37e-20	87.8
MS.gene054652.t1	MTR_2g061500	37.190	121	74	1	7	127	11	129	5.59e-18	78.6
MS.gene054652.t1	MTR_4g103530	41.573	89	52	0	57	145	5	93	3.22e-17	77.8
MS.gene054652.t1	MTR_7g106180	26.897	145	99	2	34	175	26	166	2.86e-13	67.8
MS.gene054652.t1	MTR_3g024240	33.884	121	78	1	82	200	55	175	7.81e-13	65.9
MS.gene054652.t1	MTR_7g011710	34.524	84	55	0	57	140	5	88	4.52e-12	62.8
MS.gene054652.t1	MTR_0488s0020	22.707	229	143	5	43	259	133	339	9.52e-12	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054652.t1	AT3G31430	26.961	204	143	3	14	215	112	311	1.21e-19	87.0
MS.gene054652.t1	AT2G41590	27.189	217	146	5	4	215	9	218	6.80e-19	85.1
MS.gene054652.t1	AT5G36228	23.661	224	169	1	3	226	8	229	4.62e-18	82.8
MS.gene054652.t1	AT5G18636	25.926	216	150	4	4	215	9	218	5.70e-18	82.8
MS.gene054652.t1	AT2G17920	26.852	216	148	5	4	215	9	218	5.89e-18	82.0
MS.gene054652.t1	AT5G25200	25.926	216	150	4	4	215	9	218	6.54e-18	82.4
MS.gene054652.t1	AT2G13450	26.147	218	147	4	4	215	9	218	1.14e-17	82.0
MS.gene054652.t1	AT3G57587	26.087	184	134	1	32	215	37	218	2.06e-17	80.9
MS.gene054652.t1	AT2G02103	25.463	216	151	4	4	215	9	218	2.44e-17	81.3
MS.gene054652.t1	AT3G47920	31.944	144	90	3	75	215	9	147	2.09e-14	72.0
MS.gene054652.t1	AT5G36225	33.333	90	60	0	121	210	17	106	1.12e-13	68.9
MS.gene054652.t1	AT4G02000	27.857	140	89	2	81	215	6	138	2.19e-13	68.9
MS.gene054652.t1	AT2G13500	24.336	226	166	4	4	228	9	230	3.89e-11	62.8

Find 65 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTCCAATTTGTGCCGTTT+TGG	0.219874	3.3:-84971748	MS.gene054652:CDS
TGGAAAAGATTGAAACCTTT+TGG	0.248061	3.3:-84971940	MS.gene054652:CDS
CTGGTTCTACCTCTTGAATT+TGG	0.260958	3.3:+84971900	None:intergenic
CTTGATGTCCTTCCAGAAAA+TGG	0.282882	3.3:-84971788	MS.gene054652:CDS
GAGATAATTCGAGTTGTTCT+TGG	0.302396	3.3:+84971634	None:intergenic
GAACCCAAAACGGCACAAAT+TGG	0.344104	3.3:+84971744	None:intergenic
GACCCTCACCTCCGTCTTGT+TGG	0.361586	3.3:-84972004	MS.gene054652:CDS
AAGGAAATTGCAAACTTCAT+TGG	0.378123	3.3:-84971677	MS.gene054652:CDS
TGAGGATTCTCTGTAGGTCT+AGG	0.397183	3.3:+84971849	None:intergenic
AACTGTTAAACCTTTGCTGC+TGG	0.402633	3.3:+84971322	None:intergenic
TGAATTTGGACACCTTTGAC+AGG	0.420319	3.3:+84971914	None:intergenic
AATTGGAGGTTTACCTGGCT+TGG	0.438429	3.3:+84971761	None:intergenic
CTGATGATGGAACAAGACTA+TGG	0.442111	3.3:-84971421	MS.gene054652:CDS
TTGTGAAAAGCTTTATTCCA+TGG	0.453343	3.3:-84971447	MS.gene054652:CDS
TTGGCCGCTTCCTCACTAAC+CGG	0.476388	3.3:-84971985	MS.gene054652:CDS
CTTTCTTGAGGATTCTCTGT+AGG	0.482296	3.3:+84971843	None:intergenic
TATTCCATGGAATCTGATGA+TGG	0.485852	3.3:-84971434	MS.gene054652:CDS
GTTTGTGCAGGTATGTCATC+TGG	0.494479	3.3:+84972031	None:intergenic
TGGTTTACTCGGTCATATTG+AGG	0.496082	3.3:-84971474	MS.gene054652:CDS
AGAAAATGGTGTGCCAAGCC+AGG	0.504282	3.3:-84971774	MS.gene054652:CDS
TGTTCCATCATCAGATTCCA+TGG	0.504402	3.3:+84971430	None:intergenic
CAAGTGCACGACATTCCAAC+AGG	0.508949	3.3:-84971722	MS.gene054652:CDS
TCGTGCACTTGAACCCAAAA+CGG	0.510201	3.3:+84971734	None:intergenic
ACCTTTGACAGGGTTCCAAA+AGG	0.510921	3.3:+84971925	None:intergenic
GCACAAATTGGAGGTTTACC+TGG	0.512024	3.3:+84971756	None:intergenic
TGAGGGTCTGTGGTTTGTGC+AGG	0.512257	3.3:+84972019	None:intergenic
TGTTCTTGGCGTCATATTCG+AGG	0.521779	3.3:+84971648	None:intergenic
TATGAAGATCAGAGTCCTGA+TGG	0.526288	3.3:-84971603	MS.gene054652:CDS
GTGGCTTAGTAACATCCATC+AGG	0.529918	3.3:+84971588	None:intergenic
ATCATCATGTAGGATCGTAT+TGG	0.531587	3.3:+84971962	None:intergenic
CCCAAAACGGCACAAATTGG+AGG	0.534423	3.3:+84971747	None:intergenic
CAAATTCAAGAGGTAGAACC+AGG	0.536479	3.3:-84971899	MS.gene054652:CDS
CACTCAACGAGAGATGATGA+AGG	0.539546	3.3:+84972080	None:intergenic
AATACGATCCTACATGATGA+TGG	0.539617	3.3:-84971960	MS.gene054652:CDS
GTTACAGTTTCCAGCAGCAA+AGG	0.545564	3.3:-84971332	None:intergenic
CGATTCAAGTACGAGCGGCT+AGG	0.549014	3.3:-84971515	MS.gene054652:CDS
ATGTAGCTGCATTCACCTCC+CGG	0.549990	3.3:+84971539	None:intergenic
CTGGAAACTGTAACTGTCAC+CGG	0.551548	3.3:+84971341	None:intergenic
GGCCAACAAGACGGAGGTGA+GGG	0.558506	3.3:+84972002	None:intergenic
TCACCGGTGGTTCTTGCTGC+CGG	0.563528	3.3:+84971357	None:intergenic
AATACCATGGACCTTTCTTG+AGG	0.563747	3.3:+84971831	None:intergenic
GGACATCAAGGATCAAAAGA+TGG	0.567659	3.3:+84971801	None:intergenic
CGGCCAACAAGACGGAGGTG+AGG	0.572004	3.3:+84972001	None:intergenic
AAGACGGAGGTGAGGGTCTG+TGG	0.573089	3.3:+84972009	None:intergenic
CTACAGAGAATCCTCAAGAA+AGG	0.577250	3.3:-84971842	MS.gene054652:CDS
TTGGCCGGTTAGTGAGGAAG+CGG	0.583738	3.3:+84971981	None:intergenic
ATCGTATTGGCCGGTTAGTG+AGG	0.590416	3.3:+84971975	None:intergenic
TCATCTCTCGTTGAGTGAAG+AGG	0.605479	3.3:-84972074	MS.gene054652:CDS
TCATGTAGGATCGTATTGGC+CGG	0.608829	3.3:+84971966	None:intergenic
CTCTGATCTTCATATATGAG+CGG	0.614510	3.3:+84971612	None:intergenic
GGATTCTCTGTAGGTCTAGG+TGG	0.617802	3.3:+84971852	None:intergenic
GTGCAGGTATGTCATCTGGT+AGG	0.621427	3.3:+84972035	None:intergenic
GAATCCTCAAGAAAGGTCCA+TGG	0.624128	3.3:-84971835	MS.gene054652:CDS
CAAAGGTGTCCAAATTCAAG+AGG	0.624142	3.3:-84971909	MS.gene054652:CDS
TGAGGAAGCGGCCAACAAGA+CGG	0.626222	3.3:+84971993	None:intergenic
TCGCCGGCAGCAAGAACCAC+CGG	0.628520	3.3:-84971360	MS.gene054652:CDS
GAATTTGGACACCTTTGACA+GGG	0.633819	3.3:+84971915	None:intergenic
AAAGTCAGAGAACAACTCGC+CGG	0.642877	3.3:-84971376	MS.gene054652:CDS
ACTCAACGAGAGATGATGAA+GGG	0.644189	3.3:+84972081	None:intergenic
AGATGGTTATCGAAATACCA+TGG	0.644305	3.3:+84971818	None:intergenic
TCTCTCGTTGAGTGAAGAGG+AGG	0.649737	3.3:-84972071	MS.gene054652:CDS
AATTGAAACGTATATAGACC+TGG	0.663195	3.3:+84971881	None:intergenic
ACATTCGATTCAAGTACGAG+CGG	0.719589	3.3:-84971520	MS.gene054652:CDS
GAAACTGTAACTGTCACCGG+TGG	0.726031	3.3:+84971344	None:intergenic
GGAAGCGGCCAACAAGACGG+AGG	0.736655	3.3:+84971996	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAAAAAAGATAAAGAAGC+CGG	-	chr3.3:84971863-84971882	MS.gene054652:CDS	25.0%
	AAGGAAATTGCAAACTTCAT+TGG	-	chr3.3:84971744-84971763	MS.gene054652:CDS	30.0%
	AATTGAAACGTATATAGACC+TGG	+	chr3.3:84971543-84971562	None:intergenic	30.0%
	CAAAAAAAGATAAAGAAGCC+GGG	-	chr3.3:84971864-84971883	MS.gene054652:CDS	30.0%
	GTCTTATGTCAGAAAAAGTA+GGG	-	chr3.3:84971721-84971740	MS.gene054652:CDS	30.0%
!	AATCTTTTCCATCATCATGT+AGG	+	chr3.3:84971472-84971491	None:intergenic	30.0%
!	TGGAAAAGATTGAAACCTTT+TGG	-	chr3.3:84971481-84971500	MS.gene054652:CDS	30.0%
!!	TTGTGAAAAGCTTTATTCCA+TGG	-	chr3.3:84971974-84971993	MS.gene054652:CDS	30.0%
	AATACGATCCTACATGATGA+TGG	-	chr3.3:84971461-84971480	MS.gene054652:CDS	35.0%
	AGATGGTTATCGAAATACCA+TGG	+	chr3.3:84971606-84971625	None:intergenic	35.0%
	ATCATCATGTAGGATCGTAT+TGG	+	chr3.3:84971462-84971481	None:intergenic	35.0%
	CTCTGATCTTCATATATGAG+CGG	+	chr3.3:84971812-84971831	None:intergenic	35.0%
	TATGTCAGAAAAAGTAGGGA+AGG	-	chr3.3:84971725-84971744	MS.gene054652:CDS	35.0%
	TATTCCATGGAATCTGATGA+TGG	-	chr3.3:84971987-84972006	MS.gene054652:CDS	35.0%
!	GGTCTTATGTCAGAAAAAGT+AGG	-	chr3.3:84971720-84971739	MS.gene054652:CDS	35.0%
!	TTTTCTGACATAAGACCTGT+TGG	+	chr3.3:84971717-84971736	None:intergenic	35.0%
!!	GAGATAATTCGAGTTGTTCT+TGG	+	chr3.3:84971790-84971809	None:intergenic	35.0%
!!!	ATCTTTTTTTGACGCTTCAG+TGG	+	chr3.3:84971855-84971874	None:intergenic	35.0%
!!!	GGTAATTTCTGCTACTTTTG+TGG	-	chr3.3:84971927-84971946	MS.gene054652:CDS	35.0%
!!!	TGCTACTTTTGTGGTTTACT+CGG	-	chr3.3:84971936-84971955	MS.gene054652:CDS	35.0%
	AAAAAGATAAAGAAGCCGGG+AGG	-	chr3.3:84971867-84971886	MS.gene054652:CDS	40.0%
	AATACCATGGACCTTTCTTG+AGG	+	chr3.3:84971593-84971612	None:intergenic	40.0%
	ACATTCGATTCAAGTACGAG+CGG	-	chr3.3:84971901-84971920	MS.gene054652:CDS	40.0%
	ACTCAACGAGAGATGATGAA+GGG	+	chr3.3:84971343-84971362	None:intergenic	40.0%
	CAAATTCAAGAGGTAGAACC+AGG	-	chr3.3:84971522-84971541	MS.gene054652:CDS	40.0%
	CTACAGAGAATCCTCAAGAA+AGG	-	chr3.3:84971579-84971598	MS.gene054652:CDS	40.0%
	CTTGATGTCCTTCCAGAAAA+TGG	-	chr3.3:84971633-84971652	MS.gene054652:CDS	40.0%
	CTTTCTTGAGGATTCTCTGT+AGG	+	chr3.3:84971581-84971600	None:intergenic	40.0%
	GAATTTGGACACCTTTGACA+GGG	+	chr3.3:84971509-84971528	None:intergenic	40.0%
	GGACATCAAGGATCAAAAGA+TGG	+	chr3.3:84971623-84971642	None:intergenic	40.0%
	TATGAAGATCAGAGTCCTGA+TGG	-	chr3.3:84971818-84971837	MS.gene054652:CDS	40.0%
	TGAATTTGGACACCTTTGAC+AGG	+	chr3.3:84971510-84971529	None:intergenic	40.0%
	TGGTTTACTCGGTCATATTG+AGG	-	chr3.3:84971947-84971966	MS.gene054652:CDS	40.0%
	TGTTCCATCATCAGATTCCA+TGG	+	chr3.3:84971994-84972013	None:intergenic	40.0%
!!	CAAAGGTGTCCAAATTCAAG+AGG	-	chr3.3:84971512-84971531	MS.gene054652:CDS	40.0%
!!	CATTTTCTGGAAGGACATCA+AGG	+	chr3.3:84971635-84971654	None:intergenic	40.0%
!!	CTGATGATGGAACAAGACTA+TGG	-	chr3.3:84972000-84972019	MS.gene054652:CDS	40.0%
!!	CTGGTTCTACCTCTTGAATT+TGG	+	chr3.3:84971524-84971543	None:intergenic	40.0%
	AAAGTCAGAGAACAACTCGC+CGG	-	chr3.3:84972045-84972064	MS.gene054652:CDS	45.0%
	AATTGGAGGTTTACCTGGCT+TGG	+	chr3.3:84971663-84971682	None:intergenic	45.0%
	ACCTTTGACAGGGTTCCAAA+AGG	+	chr3.3:84971499-84971518	None:intergenic	45.0%
	CACTCAACGAGAGATGATGA+AGG	+	chr3.3:84971344-84971363	None:intergenic	45.0%
	CTGGAAACTGTAACTGTCAC+CGG	+	chr3.3:84972083-84972102	None:intergenic	45.0%
	GAACCCAAAACGGCACAAAT+TGG	+	chr3.3:84971680-84971699	None:intergenic	45.0%
	GAATCCTCAAGAAAGGTCCA+TGG	-	chr3.3:84971586-84971605	MS.gene054652:CDS	45.0%
	GCACAAATTGGAGGTTTACC+TGG	+	chr3.3:84971668-84971687	None:intergenic	45.0%
	GTGGCTTAGTAACATCCATC+AGG	+	chr3.3:84971836-84971855	None:intergenic	45.0%
	GTTTGTGCAGGTATGTCATC+TGG	+	chr3.3:84971393-84971412	None:intergenic	45.0%
	TCATCTCTCGTTGAGTGAAG+AGG	-	chr3.3:84971347-84971366	MS.gene054652:CDS	45.0%
	TCGTGCACTTGAACCCAAAA+CGG	+	chr3.3:84971690-84971709	None:intergenic	45.0%
	TGAGGATTCTCTGTAGGTCT+AGG	+	chr3.3:84971575-84971594	None:intergenic	45.0%
	TGTTCTTGGCGTCATATTCG+AGG	+	chr3.3:84971776-84971795	None:intergenic	45.0%
!	TCATGTAGGATCGTATTGGC+CGG	+	chr3.3:84971458-84971477	None:intergenic	45.0%
!	TTGGCACACCATTTTCTGGA+AGG	+	chr3.3:84971644-84971663	None:intergenic	45.0%
!!!	ACCTCCAATTTGTGCCGTTT+TGG	-	chr3.3:84971673-84971692	MS.gene054652:CDS	45.0%
!!!	ACCTTTTGGAACCCTGTCAA+AGG	-	chr3.3:84971495-84971514	MS.gene054652:CDS	45.0%
!!!	CCTCCAATTTGTGCCGTTTT+GGG	-	chr3.3:84971674-84971693	MS.gene054652:CDS	45.0%
	AGAAAATGGTGTGCCAAGCC+AGG	-	chr3.3:84971647-84971666	MS.gene054652:CDS	50.0%
	ATGTAGCTGCATTCACCTCC+CGG	+	chr3.3:84971885-84971904	None:intergenic	50.0%
	CAAGTGCACGACATTCCAAC+AGG	-	chr3.3:84971699-84971718	MS.gene054652:CDS	50.0%
	CCCAAAACGGCACAAATTGG+AGG	+	chr3.3:84971677-84971696	None:intergenic	50.0%
	GAAACTGTAACTGTCACCGG+TGG	+	chr3.3:84972080-84972099	None:intergenic	50.0%
	GGATTCTCTGTAGGTCTAGG+TGG	+	chr3.3:84971572-84971591	None:intergenic	50.0%
	GTGCAGGTATGTCATCTGGT+AGG	+	chr3.3:84971389-84971408	None:intergenic	50.0%
	TCTCTCGTTGAGTGAAGAGG+AGG	-	chr3.3:84971350-84971369	MS.gene054652:CDS	50.0%
!	ATCGTATTGGCCGGTTAGTG+AGG	+	chr3.3:84971449-84971468	None:intergenic	50.0%
!!	TGGCTTGGCACACCATTTTC+TGG	+	chr3.3:84971648-84971667	None:intergenic	50.0%
	CGATTCAAGTACGAGCGGCT+AGG	-	chr3.3:84971906-84971925	MS.gene054652:CDS	55.0%
	TGAGGAAGCGGCCAACAAGA+CGG	+	chr3.3:84971431-84971450	None:intergenic	55.0%
	TTGGCCGCTTCCTCACTAAC+CGG	-	chr3.3:84971436-84971455	MS.gene054652:CDS	55.0%
	TTGGCCGGTTAGTGAGGAAG+CGG	+	chr3.3:84971443-84971462	None:intergenic	55.0%
!	TGAGGGTCTGTGGTTTGTGC+AGG	+	chr3.3:84971405-84971424	None:intergenic	55.0%
	AAGACGGAGGTGAGGGTCTG+TGG	+	chr3.3:84971415-84971434	None:intergenic	60.0%
	GACCCTCACCTCCGTCTTGT+TGG	-	chr3.3:84971417-84971436	MS.gene054652:CDS	60.0%
	GGCCAACAAGACGGAGGTGA+GGG	+	chr3.3:84971422-84971441	None:intergenic	60.0%
	TCACCGGTGGTTCTTGCTGC+CGG	+	chr3.3:84972067-84972086	None:intergenic	60.0%
	CGGCCAACAAGACGGAGGTG+AGG	+	chr3.3:84971423-84971442	None:intergenic	65.0%
	GGAAGCGGCCAACAAGACGG+AGG	+	chr3.3:84971428-84971447	None:intergenic	65.0%
	TCGCCGGCAGCAAGAACCAC+CGG	-	chr3.3:84972061-84972080	MS.gene054652:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	84971333	84972110	84971333	ID=MS.gene054652
chr3.3	mRNA	84971333	84972110	84971333	ID=MS.gene054652.t1;Parent=MS.gene054652
chr3.3	exon	84971333	84972110	84971333	ID=MS.gene054652.t1.exon1;Parent=MS.gene054652.t1
chr3.3	CDS	84971333	84972110	84971333	ID=cds.MS.gene054652.t1;Parent=MS.gene054652.t1

Gene Sequence

>MS.gene054652

ATGGCATCCCTTCATCATCTCTCGTTGAGTGAAGAGGAGGATGAACTTGAATTCCTACCAGATGACATACCTGCACAAACCACAGACCCTCACCTCCGTCTTGTTGGCCGCTTCCTCACTAACCGGCCAATACGATCCTACATGATGATGGAAAAGATTGAAACCTTTTGGAACCCTGTCAAAGGTGTCCAAATTCAAGAGGTAGAACCAGGTCTATATACGTTTCAATTCTTTCACCACCTAGACCTACAGAGAATCCTCAAGAAAGGTCCATGGTATTTCGATAACCATCTTTTGATCCTTGATGTCCTTCCAGAAAATGGTGTGCCAAGCCAGGTAAACCTCCAATTTGTGCCGTTTTGGGTTCAAGTGCACGACATTCCAACAGGTCTTATGTCAGAAAAAGTAGGGAAGGAAATTGCAAACTTCATTGGCGAATTCCTCGAATATGACGCCAAGAACAACTCGAATTATCTCCGCTCATATATGAAGATCAGAGTCCTGATGGATGTTACTAAGCCACTGAAGCGTCAAAAAAAGATAAAGAAGCCGGGAGGTGAATGCAGCTACATTCGATTCAAGTACGAGCGGCTAGGTAATTTCTGCTACTTTTGTGGTTTACTCGGTCATATTGAGGATTATTGTGAAAAGCTTTATTCCATGGAATCTGATGATGGAACAAGACTATGGAGTTCTGAACTGAGAGTCGAAAAAAGTCAGAGAACAACTCGCCGGCAGCAAGAACCACCGGTGACAGTTACAGTTTCCAGCAGCAAAG

Protein sequence

>MS.gene054652.t1

MASLHHLSLSEEEDELEFLPDDIPAQTTDPHLRLVGRFLTNRPIRSYMMMEKIETFWNPVKGVQIQEVEPGLYTFQFFHHLDLQRILKKGPWYFDNHLLILDVLPENGVPSQVNLQFVPFWVQVHDIPTGLMSEKVGKEIANFIGEFLEYDAKNNSNYLRSYMKIRVLMDVTKPLKRQKKIKKPGGECSYIRFKYERLGNFCYFCGLLGHIEDYCEKLYSMESDDGTRLWSSELRVEKSQRTTRRQQEPPVTVTVSSSK