AlfalfaGEDB | MS.gene04970

M. sativa cultivar XinJiangDaYe / MS.gene04970

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04970.t1	PNX72859.1	56.9	246	96	2	5	241	124	368	1.10E-78	303.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04970.t1	O04244	27.2	136	95	2	74	207	6	139	1.9e-10	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04970.t1	A0A2K3L2W5	56.9	246	96	2	5	241	124	368	8.1e-79	303.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04970	MS.gene041264	PPI
MS.gene43393	MS.gene04970	PPI
MS.gene04970	MS.gene03149	PPI
MS.gene34197	MS.gene04970	PPI
MS.gene04970	MS.gene29321	PPI
MS.gene005120	MS.gene04970	PPI
MS.gene04970	MS.gene025401	PPI
MS.gene04970	MS.gene015093	PPI
MS.gene048072	MS.gene04970	PPI
MS.gene060386	MS.gene04970	PPI
MS.gene71588	MS.gene04970	PPI
MS.gene04970	MS.gene24278	PPI
MS.gene04970	MS.gene000379	PPI
MS.gene04749	MS.gene04970	PPI
MS.gene04970	MS.gene34618	PPI
MS.gene04970	MS.gene59460	PPI
MS.gene05436	MS.gene04970	PPI
MS.gene04970	MS.gene66650	PPI
MS.gene059736	MS.gene04970	PPI
MS.gene70932	MS.gene04970	PPI
MS.gene35175	MS.gene04970	PPI
MS.gene04970	MS.gene08713	PPI
MS.gene04970	MS.gene09517	PPI
MS.gene04970	MS.gene87845	PPI
MS.gene021965	MS.gene04970	PPI
MS.gene055683	MS.gene04970	PPI
MS.gene042614	MS.gene04970	PPI
MS.gene021555	MS.gene04970	PPI
MS.gene04970	MS.gene58419	PPI
MS.gene04970	MS.gene26844	PPI
MS.gene04970	MS.gene64178	PPI
MS.gene04970	MS.gene074257	PPI
MS.gene04970	MS.gene44144	PPI
MS.gene009121	MS.gene04970	PPI
MS.gene04970	MS.gene05576	PPI
MS.gene94654	MS.gene04970	PPI
MS.gene04970	MS.gene060718	PPI
MS.gene36069	MS.gene04970	PPI
MS.gene04970	MS.gene48477	PPI
MS.gene04970	MS.gene027169	PPI
MS.gene04970	MS.gene004417	PPI
MS.gene83772	MS.gene04970	PPI
MS.gene04970	MS.gene019177	PPI
MS.gene008927	MS.gene04970	PPI
MS.gene045614	MS.gene04970	PPI
MS.gene96541	MS.gene04970	PPI
MS.gene000634	MS.gene04970	PPI
MS.gene63762	MS.gene04970	PPI
MS.gene04970	MS.gene055560	PPI
MS.gene04970	MS.gene024297	PPI
MS.gene30208	MS.gene04970	PPI
MS.gene025873	MS.gene04970	PPI
MS.gene04970	MS.gene054652	PPI
MS.gene04970	MS.gene37537	PPI
MS.gene013676	MS.gene04970	PPI
MS.gene04970	MS.gene069786	PPI
MS.gene05358	MS.gene04970	PPI
MS.gene04970	MS.gene42010	PPI
MS.gene04970	MS.gene025158	PPI
MS.gene030090	MS.gene04970	PPI
MS.gene95561	MS.gene04970	PPI
MS.gene96174	MS.gene04970	PPI
MS.gene04970	MS.gene27329	PPI
MS.gene018196	MS.gene04970	PPI
MS.gene04970	MS.gene41393	PPI
MS.gene065627	MS.gene04970	PPI
MS.gene57019	MS.gene04970	PPI
MS.gene018745	MS.gene04970	PPI
MS.gene04970	MS.gene016632	PPI
MS.gene04970	MS.gene018474	PPI
MS.gene65546	MS.gene04970	PPI
MS.gene03936	MS.gene04970	PPI
MS.gene04970	MS.gene40469	PPI
MS.gene04970	MS.gene96163	PPI
MS.gene038098	MS.gene04970	PPI
MS.gene60059	MS.gene04970	PPI
MS.gene28545	MS.gene04970	PPI
MS.gene28718	MS.gene04970	PPI
MS.gene008275	MS.gene04970	PPI
MS.gene038757	MS.gene04970	PPI
MS.gene064966	MS.gene04970	PPI
MS.gene04970	MS.gene037049	PPI
MS.gene04970	MS.gene026680	PPI
MS.gene04970	MS.gene61499	PPI
MS.gene04970	MS.gene019165	PPI
MS.gene04970	MS.gene81194	PPI
MS.gene04970	MS.gene060773	PPI
MS.gene43924	MS.gene04970	PPI
MS.gene046089	MS.gene04970	PPI
MS.gene047954	MS.gene04970	PPI
MS.gene036423	MS.gene04970	PPI
MS.gene04970	MS.gene038394	PPI
MS.gene04970	MS.gene019001	PPI
MS.gene04970	MS.gene53904	PPI
MS.gene04970	MS.gene24070	PPI
MS.gene04970	MS.gene73261	PPI
MS.gene72520	MS.gene04970	PPI
MS.gene04970	MS.gene85538	PPI
MS.gene04970	MS.gene025675	PPI
MS.gene66651	MS.gene04970	PPI
MS.gene96109	MS.gene04970	PPI
MS.gene04970	MS.gene055546	PPI
MS.gene04970	MS.gene71637	PPI
MS.gene04970	MS.gene55209	PPI
MS.gene35202	MS.gene04970	PPI
MS.gene051449	MS.gene04970	PPI
MS.gene04970	MS.gene073573	PPI
MS.gene63743	MS.gene04970	PPI
MS.gene04970	MS.gene28718	PPI
MS.gene48477	MS.gene04970	PPI
MS.gene30136	MS.gene04970	PPI
MS.gene04970	MS.gene068629	PPI
MS.gene008861	MS.gene04970	PPI
MS.gene61452	MS.gene04970	PPI
MS.gene04970	MS.gene009781	PPI
MS.gene83979	MS.gene04970	PPI
MS.gene016102	MS.gene04970	PPI
MS.gene46941	MS.gene04970	PPI
MS.gene85295	MS.gene04970	PPI
MS.gene04970	MS.gene21066	PPI
MS.gene04970	MS.gene009213	PPI
MS.gene04970	MS.gene013890	PPI
MS.gene47792	MS.gene04970	PPI
MS.gene04970	MS.gene98127	PPI
MS.gene46948	MS.gene04970	PPI
MS.gene04970	MS.gene073582	PPI
MS.gene98471	MS.gene04970	PPI
MS.gene04970	MS.gene003264	PPI
MS.gene012019	MS.gene04970	PPI
MS.gene04970	MS.gene048706	PPI
MS.gene93396	MS.gene04970	PPI
MS.gene04970	MS.gene45083	PPI
MS.gene04970	MS.gene049561	PPI
MS.gene04970	MS.gene008658	PPI
MS.gene05231	MS.gene04970	PPI
MS.gene04970	MS.gene80362	PPI
MS.gene04970	MS.gene49057	PPI
MS.gene04970	MS.gene89815	PPI
MS.gene010656	MS.gene04970	PPI
MS.gene000629	MS.gene04970	PPI
MS.gene04970	MS.gene048088	PPI
MS.gene04970	MS.gene86868	PPI
MS.gene041264	MS.gene04970	PPI
MS.gene41979	MS.gene04970	PPI
MS.gene04970	MS.gene058889	PPI
MS.gene64130	MS.gene04970	PPI
MS.gene05319	MS.gene04970	PPI
MS.gene56991	MS.gene04970	PPI
MS.gene050679	MS.gene04970	PPI
MS.gene04970	MS.gene034643	PPI
MS.gene20246	MS.gene04970	PPI
MS.gene055223	MS.gene04970	PPI
MS.gene053747	MS.gene04970	PPI
MS.gene04970	MS.gene012889	PPI
MS.gene04970	MS.gene34463	PPI
MS.gene04970	MS.gene038757	PPI
MS.gene04970	MS.gene000506	PPI
MS.gene65471	MS.gene04970	PPI
MS.gene061850	MS.gene04970	PPI
MS.gene26433	MS.gene04970	PPI
MS.gene04970	MS.gene51804	PPI
MS.gene04970	MS.gene013644	PPI
MS.gene04970	MS.gene54051	PPI
MS.gene98616	MS.gene04970	PPI
MS.gene042027	MS.gene04970	PPI
MS.gene04970	MS.gene010890	PPI
MS.gene69490	MS.gene04970	PPI
MS.gene04970	MS.gene061579	PPI
MS.gene015248	MS.gene04970	PPI
MS.gene80362	MS.gene04970	PPI
MS.gene44083	MS.gene04970	PPI
MS.gene013193	MS.gene04970	PPI
MS.gene08106	MS.gene04970	PPI
MS.gene58419	MS.gene04970	PPI
MS.gene04970	MS.gene060788	PPI
MS.gene04970	MS.gene43924	PPI
MS.gene04970	MS.gene41643	PPI
MS.gene04970	MS.gene027994	PPI
MS.gene23521	MS.gene04970	PPI
MS.gene04970	MS.gene022929	PPI
MS.gene04970	MS.gene26433	PPI
MS.gene04970	MS.gene007022	PPI
MS.gene29406	MS.gene04970	PPI
MS.gene04970	MS.gene69584	PPI
MS.gene04970	MS.gene52349	PPI
MS.gene87529	MS.gene04970	PPI
MS.gene024405	MS.gene04970	PPI
MS.gene04970	MS.gene021647	PPI
MS.gene012748	MS.gene04970	PPI
MS.gene04134	MS.gene04970	PPI
MS.gene062067	MS.gene04970	PPI
MS.gene04970	MS.gene048342	PPI
MS.gene04970	MS.gene069624	PPI
MS.gene57044	MS.gene04970	PPI
MS.gene038676	MS.gene04970	PPI
MS.gene04970	MS.gene016719	PPI
MS.gene04970	MS.gene028714	PPI
MS.gene71637	MS.gene04970	PPI
MS.gene04970	MS.gene88393	PPI
MS.gene04970	MS.gene054508	PPI
MS.gene042651	MS.gene04970	PPI
MS.gene04970	MS.gene69586	PPI
MS.gene04970	MS.gene053373	PPI
MS.gene05576	MS.gene04970	PPI
MS.gene04970	MS.gene63150	PPI
MS.gene04970	MS.gene03936	PPI
MS.gene034643	MS.gene04970	PPI
MS.gene04970	MS.gene072852	PPI
MS.gene04970	MS.gene65474	PPI
MS.gene04970	MS.gene08708	PPI
MS.gene04970	MS.gene06164	PPI
MS.gene04970	MS.gene50392	PPI
MS.gene04970	MS.gene86275	PPI
MS.gene04970	MS.gene22730	PPI
MS.gene04970	MS.gene032299	PPI
MS.gene04970	MS.gene017586	PPI
MS.gene04970	MS.gene037634	PPI
MS.gene04970	MS.gene057828	PPI
MS.gene04970	MS.gene08606	PPI
MS.gene071784	MS.gene04970	PPI
MS.gene061579	MS.gene04970	PPI
MS.gene04970	MS.gene000640	PPI
MS.gene76420	MS.gene04970	PPI
MS.gene071320	MS.gene04970	PPI
MS.gene04970	MS.gene98756	PPI
MS.gene42903	MS.gene04970	PPI
MS.gene81712	MS.gene04970	PPI
MS.gene026680	MS.gene04970	PPI
MS.gene04970	MS.gene028716	PPI
MS.gene04970	MS.gene74639	PPI
MS.gene04970	MS.gene071133	PPI
MS.gene04970	MS.gene60266	PPI
MS.gene04970	MS.gene98467	PPI
MS.gene048747	MS.gene04970	PPI
MS.gene04970	MS.gene62249	PPI
MS.gene04970	MS.gene024522	PPI
MS.gene042559	MS.gene04970	PPI
MS.gene04970	MS.gene22111	PPI
MS.gene22200	MS.gene04970	PPI
MS.gene04970	MS.gene049610	PPI
MS.gene04970	MS.gene010290	PPI
MS.gene04970	MS.gene37955	PPI
MS.gene26038	MS.gene04970	PPI
MS.gene04970	MS.gene92517	PPI
MS.gene04970	MS.gene36069	PPI
MS.gene85308	MS.gene04970	PPI
MS.gene04970	MS.gene045875	PPI
MS.gene04970	MS.gene84790	PPI
MS.gene04970	MS.gene044729	PPI
MS.gene031967	MS.gene04970	PPI
MS.gene04970	MS.gene60390	PPI
MS.gene34812	MS.gene04970	PPI
MS.gene04970	MS.gene039692	PPI
MS.gene02896	MS.gene04970	PPI
MS.gene063214	MS.gene04970	PPI
MS.gene04970	MS.gene55682	PPI
MS.gene04970	MS.gene002590	PPI
MS.gene97410	MS.gene04970	PPI
MS.gene32415	MS.gene04970	PPI
MS.gene035626	MS.gene04970	PPI
MS.gene067340	MS.gene04970	PPI
MS.gene04970	MS.gene77990	PPI
MS.gene04970	MS.gene062511	PPI
MS.gene65623	MS.gene04970	PPI
MS.gene71675	MS.gene04970	PPI
MS.gene04970	MS.gene059269	PPI
MS.gene69495	MS.gene04970	PPI
MS.gene049512	MS.gene04970	PPI
MS.gene04970	MS.gene057934	PPI
MS.gene04970	MS.gene024841	PPI
MS.gene038623	MS.gene04970	PPI
MS.gene05357	MS.gene04970	PPI
MS.gene032311	MS.gene04970	PPI
MS.gene04970	MS.gene76420	PPI
MS.gene04970	MS.gene46353	PPI
MS.gene04970	MS.gene40828	PPI
MS.gene86849	MS.gene04970	PPI
MS.gene04970	MS.gene51799	PPI
MS.gene04970	MS.gene98471	PPI
MS.gene04970	MS.gene61651	PPI
MS.gene04970	MS.gene29406	PPI
MS.gene39956	MS.gene04970	PPI
MS.gene021968	MS.gene04970	PPI
MS.gene04970	MS.gene09023	PPI
MS.gene09589	MS.gene04970	PPI
MS.gene04970	MS.gene025987	PPI
MS.gene04970	MS.gene95960	PPI
MS.gene04970	MS.gene043140	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04970.t1	MTR_3g053920	52.400	250	45	3	1	241	1	185	8.35e-83	249
MS.gene04970.t1	MTR_2g061500	70.543	129	29	1	1	120	1	129	3.65e-62	191
MS.gene04970.t1	MTR_4g103530	68.041	97	22	1	55	142	1	97	2.54e-42	142
MS.gene04970.t1	MTR_6g033215	34.010	197	114	4	5	190	103	294	2.15e-34	125
MS.gene04970.t1	MTR_7g011710	64.706	85	21	1	55	130	1	85	3.75e-32	115
MS.gene04970.t1	MTR_0010s0370	32.955	176	103	5	7	168	3	177	9.33e-27	102
MS.gene04970.t1	MTR_0010s0350	32.955	176	103	5	7	168	3	177	9.33e-27	102

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04970.t1	AT2G17920	26.429	140	99	2	70	207	82	219	8.50e-17	78.2
MS.gene04970.t1	AT5G36228	26.875	160	110	3	64	218	76	233	1.01e-14	72.8
MS.gene04970.t1	AT2G41590	26.761	142	96	3	70	207	82	219	1.27e-14	72.8
MS.gene04970.t1	AT3G31430	26.761	213	137	6	8	206	104	311	1.66e-14	72.0
MS.gene04970.t1	AT2G13450	26.776	183	121	4	35	206	38	218	1.87e-14	72.4
MS.gene04970.t1	AT3G57587	25.000	140	101	2	70	207	82	219	2.08e-14	72.0
MS.gene04970.t1	AT5G25200	26.389	144	94	4	70	207	82	219	1.94e-13	69.3
MS.gene04970.t1	AT5G18636	26.389	144	94	4	70	207	82	219	2.00e-13	69.3
MS.gene04970.t1	AT2G02103	26.761	142	96	3	70	207	82	219	2.55e-13	68.9
MS.gene04970.t1	AT4G02000	27.206	136	95	2	74	207	6	139	2.66e-12	65.5
MS.gene04970.t1	AT3G47920	25.714	140	100	2	70	207	11	148	3.55e-12	65.1

Find 83 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGATGATGGTGTGCGCAAC+TGG	0.191583	3.3:-58999653	MS.gene04970:CDS
CTGCTTGATCGGTCGTGTTT+TGG	0.246456	3.3:-59000192	MS.gene04970:CDS
CATTGAATCACCTGGACATT+TGG	0.249024	3.3:-58999974	MS.gene04970:CDS
ATCCTACAACCACTTGTTAT+TGG	0.270769	3.3:+58999587	None:intergenic
TGTTACTGTACCACTGAAAA+AGG	0.308378	3.3:-58999811	MS.gene04970:CDS
CAAGTCCACGGTTTGCCCTT+TGG	0.326527	3.3:-58999948	MS.gene04970:CDS
CATCAAGTTTGTGGTGAAAC+TGG	0.331056	3.3:+59000090	None:intergenic
GCTTGCCTGGTTTCCATGCT+CGG	0.336051	3.3:+59000126	None:intergenic
CAATGTCGGCAGCTAGGTTA+AGG	0.345236	3.3:+59000237	None:intergenic
CAAGGAATTGGCAGTTTCCT+TGG	0.351997	3.3:-58999903	MS.gene04970:CDS
ATTGAATCACCTGGACATTT+GGG	0.353010	3.3:-58999973	MS.gene04970:CDS
CCAATTCCTTGACCAACCTT+TGG	0.354457	3.3:+58999915	None:intergenic
CCAAAGGTTGGTCAAGGAAT+TGG	0.363573	3.3:-58999915	MS.gene04970:CDS
TTCTCATATTTAAACAAAAC+AGG	0.373656	3.3:+58999753	None:intergenic
TGTTACAGGTGTGGCATTCT+CGG	0.390750	3.3:-58999717	MS.gene04970:CDS
CCTCACTTTGAGTCGCATAA+AGG	0.417235	3.3:+58999839	None:intergenic
CCCACGAACCTTAACATTGA+TGG	0.418920	3.3:-59000287	MS.gene04970:CDS
TGACAACTTTCATATAGTTA+TGG	0.430441	3.3:-59000033	MS.gene04970:CDS
GTGTCCGGAGCTGTTCGAAC+TGG	0.431938	3.3:-58999679	MS.gene04970:CDS
GACCGATCAAGCAGTGTTCA+AGG	0.432003	3.3:+59000201	None:intergenic
GACCCTATGCGTGTAGAAAC+AGG	0.433793	3.3:+58999615	None:intergenic
TGATGATGGTGTGCGCAACT+GGG	0.435685	3.3:-58999652	MS.gene04970:CDS
AGGAACTGTGAATGGTATTA+CGG	0.436819	3.3:+58999859	None:intergenic
GTGTGGCATTCTCGGTCATT+CGG	0.451796	3.3:-58999709	MS.gene04970:CDS
ATGATTGTCACACGCTTGCC+TGG	0.452076	3.3:+59000113	None:intergenic
CTTGCCTGGTTTCCATGCTC+GGG	0.461634	3.3:+59000127	None:intergenic
CACGAGCAACATCAAGTTTG+TGG	0.464404	3.3:+59000081	None:intergenic
GTAGAAACAGGTTTAAGGTC+AGG	0.470530	3.3:+58999627	None:intergenic
ACCATCAATGTTAAGGTTCG+TGG	0.472453	3.3:+59000286	None:intergenic
ATCCTTGAACACTGCTTGAT+CGG	0.474216	3.3:-59000203	MS.gene04970:CDS
GGGTAAGACCATCAATGTTA+AGG	0.477421	3.3:+59000279	None:intergenic
GGGTCAAACCCTCATCAGCC+AGG	0.480642	3.3:+59000258	None:intergenic
TGCGTGTAGAAACAGGTTTA+AGG	0.481272	3.3:+58999622	None:intergenic
CAAATGTCCAGGTGATTCAA+TGG	0.494410	3.3:+58999975	None:intergenic
TCATTCGGACAAGGTGTGTC+CGG	0.496311	3.3:-58999694	MS.gene04970:CDS
AATGTCGGCAGCTAGGTTAA+GGG	0.497192	3.3:+59000238	None:intergenic
CTTTGGATTCATTCAACCAA+AGG	0.499724	3.3:-58999931	MS.gene04970:CDS
TGGTCTTACCCTGGCTGATG+AGG	0.501493	3.3:-59000267	MS.gene04970:CDS
CACTGTTCCATTGAATCACC+TGG	0.504618	3.3:-58999982	MS.gene04970:CDS
GTTAAGGTTCGTGGGATCCA+TGG	0.509655	3.3:+59000295	None:intergenic
AGCGGCTTTGATTTCTCGAT+CGG	0.509809	3.3:+59000169	None:intergenic
GACATTTGGGTGCAAGTCCA+CGG	0.511650	3.3:-58999960	MS.gene04970:CDS
TCGCATAAAGGAACTGTGAA+TGG	0.520321	3.3:+58999851	None:intergenic
GTGTGTTGGACGAGGGTCCA+TGG	0.521077	3.3:-59000061	MS.gene04970:CDS
TGTTGCTCGTGTGTTGGACG+AGG	0.522450	3.3:-59000069	MS.gene04970:CDS
AAACCTGTTTCTACACGCAT+AGG	0.525206	3.3:-58999618	MS.gene04970:CDS
GGATTCATTCAACCAAAGGT+TGG	0.527032	3.3:-58999927	MS.gene04970:CDS
GGTCTTACCCTGGCTGATGA+GGG	0.530738	3.3:-59000266	MS.gene04970:CDS
GAGTCCAGTTCGAACAGCTC+CGG	0.535698	3.3:+58999675	None:intergenic
AACCTGTTTCTACACGCATA+GGG	0.536848	3.3:-58999617	MS.gene04970:CDS
ATGTCCCGAGCATGGAAACC+AGG	0.539941	3.3:-59000131	MS.gene04970:CDS
TGCCAATAACAAGTGGTTGT+AGG	0.543586	3.3:-58999589	None:intergenic
TTCGAACTGGACTCTGATGA+TGG	0.548007	3.3:-58999666	MS.gene04970:CDS
GGACTTGCACCCAAATGTCC+AGG	0.551238	3.3:+58999964	None:intergenic
GATCAAGCAGTGTTCAAGGA+TGG	0.553362	3.3:+59000205	None:intergenic
ATTCAACCAAAGGTTGGTCA+AGG	0.554054	3.3:-58999921	MS.gene04970:CDS
GTTATGGAACGTATTGCTCC+TGG	0.556965	3.3:-59000017	MS.gene04970:CDS
TTTGGTTGAATGAATCCAAA+GGG	0.557289	3.3:+58999933	None:intergenic
ATCAAGCAGTGTTCAAGGAT+GGG	0.561405	3.3:+59000206	None:intergenic
CATTCTCGGTCATTCGGACA+AGG	0.565279	3.3:-58999703	MS.gene04970:CDS
ACTTGATGTTGCTCGTGTGT+TGG	0.565320	3.3:-59000075	MS.gene04970:CDS
AGGACTCAATGTCGGCAGCT+AGG	0.567310	3.3:+59000231	None:intergenic
TTGTTTAAATATGAGAAGCT+TGG	0.568801	3.3:-58999747	MS.gene04970:CDS
TAGAAACAGGTTTAAGGTCA+GGG	0.570596	3.3:+58999628	None:intergenic
CCATCAATGTTAAGGTTCGT+GGG	0.580051	3.3:+59000287	None:intergenic
ACAGTGGTCGAGACAACACC+AGG	0.584103	3.3:+58999999	None:intergenic
GATGATGGTGTGCGCAACTG+GGG	0.588816	3.3:-58999651	MS.gene04970:CDS
CTTTGGTTGAATGAATCCAA+AGG	0.593606	3.3:+58999932	None:intergenic
GTGAACGCATGTCCCGAGCA+TGG	0.594541	3.3:-59000139	MS.gene04970:CDS
CCTTTATGCGACTCAAAGTG+AGG	0.598255	3.3:-58999839	MS.gene04970:CDS
TGAAAGTTGTCATATAGCCA+TGG	0.616309	3.3:+59000044	None:intergenic
GGTCAAACCCTCATCAGCCA+GGG	0.622364	3.3:+59000259	None:intergenic
TAACATTGATGGTCTTACCC+TGG	0.623982	3.3:-59000276	MS.gene04970:CDS
TGGAAAGTCTGTGCTAACAA+TGG	0.635396	3.3:-58999786	MS.gene04970:CDS
GATGGGAGAGGACTCAATGT+CGG	0.638132	3.3:+59000223	None:intergenic
TGTTTCTACACGCATAGGGT+CGG	0.642624	3.3:-58999613	MS.gene04970:CDS
GTTGCTCGTGTGTTGGACGA+GGG	0.650160	3.3:-59000068	MS.gene04970:CDS
CATGCGTTCACTGAAGTAAG+CGG	0.663600	3.3:+59000151	None:intergenic
GCAGTGTTCAAGGATGGGAG+AGG	0.664768	3.3:+59000211	None:intergenic
GGTCGGCTGCCAATAACAAG+TGG	0.671622	3.3:-58999596	MS.gene04970:CDS
TGAATCCAAAGGGCAAACCG+TGG	0.676765	3.3:+58999943	None:intergenic
CAGGTGATTCAATGGAACAG+TGG	0.687191	3.3:+58999983	None:intergenic
CATATGTGTGTAAAGCACCA+AGG	0.691205	3.3:+58999886	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCTCATATTTAAACAAAAC+AGG	+	chr3.3:59000133-59000152	None:intergenic	20.0%
!	TGACAACTTTCATATAGTTA+TGG	-	chr3.3:58999850-58999869	MS.gene04970:CDS	25.0%
!	TTGTTTAAATATGAGAAGCT+TGG	-	chr3.3:59000136-59000155	MS.gene04970:CDS	25.0%
	TTTGGTTGAATGAATCCAAA+GGG	+	chr3.3:58999953-58999972	None:intergenic	30.0%
	AGGAACTGTGAATGGTATTA+CGG	+	chr3.3:59000027-59000046	None:intergenic	35.0%
	ATTGAATCACCTGGACATTT+GGG	-	chr3.3:58999910-58999929	MS.gene04970:CDS	35.0%
	CTTTGGATTCATTCAACCAA+AGG	-	chr3.3:58999952-58999971	MS.gene04970:CDS	35.0%
	CTTTGGTTGAATGAATCCAA+AGG	+	chr3.3:58999954-58999973	None:intergenic	35.0%
	TGAAAGTTGTCATATAGCCA+TGG	+	chr3.3:58999842-58999861	None:intergenic	35.0%
	TGTTACTGTACCACTGAAAA+AGG	-	chr3.3:59000072-59000091	MS.gene04970:CDS	35.0%
!	TAGAAACAGGTTTAAGGTCA+GGG	+	chr3.3:59000258-59000277	None:intergenic	35.0%
!!!	AGCTTGGTGTTTTTTGTTAC+AGG	-	chr3.3:59000152-59000171	MS.gene04970:CDS	35.0%
	ACCATCAATGTTAAGGTTCG+TGG	+	chr3.3:58999600-58999619	None:intergenic	40.0%
	ATCAAGCAGTGTTCAAGGAT+GGG	+	chr3.3:58999680-58999699	None:intergenic	40.0%
	ATCCTTGAACACTGCTTGAT+CGG	-	chr3.3:58999680-58999699	MS.gene04970:CDS	40.0%
	CAAATGTCCAGGTGATTCAA+TGG	+	chr3.3:58999911-58999930	None:intergenic	40.0%
	CATCAAGTTTGTGGTGAAAC+TGG	+	chr3.3:58999796-58999815	None:intergenic	40.0%
	CATTGAATCACCTGGACATT+TGG	-	chr3.3:58999909-58999928	MS.gene04970:CDS	40.0%
	CCATCAATGTTAAGGTTCGT+GGG	+	chr3.3:58999599-58999618	None:intergenic	40.0%
	GGATTCATTCAACCAAAGGT+TGG	-	chr3.3:58999956-58999975	MS.gene04970:CDS	40.0%
	GGGTAAGACCATCAATGTTA+AGG	+	chr3.3:58999607-58999626	None:intergenic	40.0%
	TCGCATAAAGGAACTGTGAA+TGG	+	chr3.3:59000035-59000054	None:intergenic	40.0%
	TGCGTGTAGAAACAGGTTTA+AGG	+	chr3.3:59000264-59000283	None:intergenic	40.0%
	TGGAAAGTCTGTGCTAACAA+TGG	-	chr3.3:59000097-59000116	MS.gene04970:CDS	40.0%
!	ATTCAACCAAAGGTTGGTCA+AGG	-	chr3.3:58999962-58999981	MS.gene04970:CDS	40.0%
!	CATATGTGTGTAAAGCACCA+AGG	+	chr3.3:59000000-59000019	None:intergenic	40.0%
!	GTAGAAACAGGTTTAAGGTC+AGG	+	chr3.3:59000259-59000278	None:intergenic	40.0%
!	TAACATTGATGGTCTTACCC+TGG	-	chr3.3:58999607-58999626	MS.gene04970:CDS	40.0%
!!	AAACCTGTTTCTACACGCAT+AGG	-	chr3.3:59000265-59000284	MS.gene04970:CDS	40.0%
!!	AACCTGTTTCTACACGCATA+GGG	-	chr3.3:59000266-59000285	MS.gene04970:CDS	40.0%
!!!	ACTTTCCACTCCTTTTTCAG+TGG	+	chr3.3:59000085-59000104	None:intergenic	40.0%
!!!	GGTGTTTTTTGTTACAGGTG+TGG	-	chr3.3:59000157-59000176	MS.gene04970:CDS	40.0%
	AATGTCGGCAGCTAGGTTAA+GGG	+	chr3.3:58999648-58999667	None:intergenic	45.0%
	ACTTGATGTTGCTCGTGTGT+TGG	-	chr3.3:58999808-58999827	MS.gene04970:CDS	45.0%
	CAAGGAATTGGCAGTTTCCT+TGG	-	chr3.3:58999980-58999999	MS.gene04970:CDS	45.0%
	CACGAGCAACATCAAGTTTG+TGG	+	chr3.3:58999805-58999824	None:intergenic	45.0%
	CACTGTTCCATTGAATCACC+TGG	-	chr3.3:58999901-58999920	MS.gene04970:CDS	45.0%
	CAGGTGATTCAATGGAACAG+TGG	+	chr3.3:58999903-58999922	None:intergenic	45.0%
	CATGCGTTCACTGAAGTAAG+CGG	+	chr3.3:58999735-58999754	None:intergenic	45.0%
	CCAATTCCTTGACCAACCTT+TGG	+	chr3.3:58999971-58999990	None:intergenic	45.0%
	CCCACGAACCTTAACATTGA+TGG	-	chr3.3:58999596-58999615	MS.gene04970:CDS	45.0%
	CCTCACTTTGAGTCGCATAA+AGG	+	chr3.3:59000047-59000066	None:intergenic	45.0%
	CCTTTATGCGACTCAAAGTG+AGG	-	chr3.3:59000044-59000063	MS.gene04970:CDS	45.0%
	CTGTACCACTGAAAAAGGAG+TGG	-	chr3.3:59000077-59000096	MS.gene04970:CDS	45.0%
	GATCAAGCAGTGTTCAAGGA+TGG	+	chr3.3:58999681-58999700	None:intergenic	45.0%
	GTTATGGAACGTATTGCTCC+TGG	-	chr3.3:58999866-58999885	MS.gene04970:CDS	45.0%
	TGTTACAGGTGTGGCATTCT+CGG	-	chr3.3:59000166-59000185	MS.gene04970:CDS	45.0%
	TTCGAACTGGACTCTGATGA+TGG	-	chr3.3:59000217-59000236	MS.gene04970:CDS	45.0%
!	AGCGGCTTTGATTTCTCGAT+CGG	+	chr3.3:58999717-58999736	None:intergenic	45.0%
!	CCAAAGGTTGGTCAAGGAAT+TGG	-	chr3.3:58999968-58999987	MS.gene04970:CDS	45.0%
!	TGTTTCTACACGCATAGGGT+CGG	-	chr3.3:59000270-59000289	MS.gene04970:CDS	45.0%
	ATGATTGTCACACGCTTGCC+TGG	+	chr3.3:58999773-58999792	None:intergenic	50.0%
	CAATGTCGGCAGCTAGGTTA+AGG	+	chr3.3:58999649-58999668	None:intergenic	50.0%
	CATTCTCGGTCATTCGGACA+AGG	-	chr3.3:59000180-59000199	MS.gene04970:CDS	50.0%
	GACATTTGGGTGCAAGTCCA+CGG	-	chr3.3:58999923-58999942	MS.gene04970:CDS	50.0%
	GACCCTATGCGTGTAGAAAC+AGG	+	chr3.3:59000271-59000290	None:intergenic	50.0%
	GACCGATCAAGCAGTGTTCA+AGG	+	chr3.3:58999685-58999704	None:intergenic	50.0%
	GATGGGAGAGGACTCAATGT+CGG	+	chr3.3:58999663-58999682	None:intergenic	50.0%
	GTGTGGCATTCTCGGTCATT+CGG	-	chr3.3:59000174-59000193	MS.gene04970:CDS	50.0%
	TCATTCGGACAAGGTGTGTC+CGG	-	chr3.3:59000189-59000208	MS.gene04970:CDS	50.0%
	TGAATCCAAAGGGCAAACCG+TGG	+	chr3.3:58999943-58999962	None:intergenic	50.0%
!	CTGCTTGATCGGTCGTGTTT+TGG	-	chr3.3:58999691-58999710	MS.gene04970:CDS	50.0%
!!	TGATGATGGTGTGCGCAACT+GGG	-	chr3.3:59000231-59000250	MS.gene04970:CDS	50.0%
	ACAGTGGTCGAGACAACACC+AGG	+	chr3.3:58999887-58999906	None:intergenic	55.0%
	AGGACTCAATGTCGGCAGCT+AGG	+	chr3.3:58999655-58999674	None:intergenic	55.0%
	ATGTCCCGAGCATGGAAACC+AGG	-	chr3.3:58999752-58999771	MS.gene04970:CDS	55.0%
	CAAGTCCACGGTTTGCCCTT+TGG	-	chr3.3:58999935-58999954	MS.gene04970:CDS	55.0%
	CTTGCCTGGTTTCCATGCTC+GGG	+	chr3.3:58999759-58999778	None:intergenic	55.0%
	GAGTCCAGTTCGAACAGCTC+CGG	+	chr3.3:59000211-59000230	None:intergenic	55.0%
	GCAGTGTTCAAGGATGGGAG+AGG	+	chr3.3:58999675-58999694	None:intergenic	55.0%
	GCTTGCCTGGTTTCCATGCT+CGG	+	chr3.3:58999760-58999779	None:intergenic	55.0%
	GGACTTGCACCCAAATGTCC+AGG	+	chr3.3:58999922-58999941	None:intergenic	55.0%
	GGTCAAACCCTCATCAGCCA+GGG	+	chr3.3:58999627-58999646	None:intergenic	55.0%
	GGTCGGCTGCCAATAACAAG+TGG	-	chr3.3:59000287-59000306	MS.gene04970:CDS	55.0%
	GGTCTTACCCTGGCTGATGA+GGG	-	chr3.3:58999617-58999636	MS.gene04970:CDS	55.0%
	GTTGCTCGTGTGTTGGACGA+GGG	-	chr3.3:58999815-58999834	MS.gene04970:CDS	55.0%
	TGTTGCTCGTGTGTTGGACG+AGG	-	chr3.3:58999814-58999833	MS.gene04970:CDS	55.0%
!	GATGATGGTGTGCGCAACTG+GGG	-	chr3.3:59000232-59000251	MS.gene04970:CDS	55.0%
!	TGGTCTTACCCTGGCTGATG+AGG	-	chr3.3:58999616-58999635	MS.gene04970:CDS	55.0%
!!	CTGATGATGGTGTGCGCAAC+TGG	-	chr3.3:59000230-59000249	MS.gene04970:CDS	55.0%
	GGGTCAAACCCTCATCAGCC+AGG	+	chr3.3:58999628-58999647	None:intergenic	60.0%
	GTGAACGCATGTCCCGAGCA+TGG	-	chr3.3:58999744-58999763	MS.gene04970:CDS	60.0%
	GTGTCCGGAGCTGTTCGAAC+TGG	-	chr3.3:59000204-59000223	MS.gene04970:CDS	60.0%
	GTGTGTTGGACGAGGGTCCA+TGG	-	chr3.3:58999822-58999841	MS.gene04970:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	58999590	59000315	58999590	ID=MS.gene04970
chr3.3	mRNA	58999590	59000315	58999590	ID=MS.gene04970.t1;Parent=MS.gene04970
chr3.3	exon	58999590	59000315	58999590	ID=MS.gene04970.t1.exon1;Parent=MS.gene04970.t1
chr3.3	CDS	58999590	59000315	58999590	ID=cds.MS.gene04970.t1;Parent=MS.gene04970.t1

Gene Sequence

>MS.gene04970

ATGGATCCCACGAACCTTAACATTGATGGTCTTACCCTGGCTGATGAGGGTTTGACCCTTAACCTAGCTGCCGACATTGAGTCCTCTCCCATCCTTGAACACTGCTTGATCGGTCGTGTTTTGGCCGATCGAGAAATCAAAGCCGCTTACTTCAGTGAACGCATGTCCCGAGCATGGAAACCAGGCAAGCGTGTGACAATCATCCAGTTTCACCACAAACTTGATGTTGCTCGTGTGTTGGACGAGGGTCCATGGCTATATGACAACTTTCATATAGTTATGGAACGTATTGCTCCTGGTGTTGTCTCGACCACTGTTCCATTGAATCACCTGGACATTTGGGTGCAAGTCCACGGTTTGCCCTTTGGATTCATTCAACCAAAGGTTGGTCAAGGAATTGGCAGTTTCCTTGGTGCTTTACACACATATGATAGCCGTAATACCATTCACAGTTCCTTTATGCGACTCAAAGTGAGGATTGATGTTACTGTACCACTGAAAAAGGAGTGGAAAGTCTGTGCTAACAATGGTTCTTACGTTCCTGTTTTGTTTAAATATGAGAAGCTTGGTGTTTTTTGTTACAGGTGTGGCATTCTCGGTCATTCGGACAAGGTGTGTCCGGAGCTGTTCGAACTGGACTCTGATGATGGTGTGCGCAACTGGGGCCCTGACCTTAAACCTGTTTCTACACGCATAGGGTCGGCTGCCAATAACAAGTGGTTGTAG

Protein sequence

>MS.gene04970.t1

MDPTNLNIDGLTLADEGLTLNLAADIESSPILEHCLIGRVLADREIKAAYFSERMSRAWKPGKRVTIIQFHHKLDVARVLDEGPWLYDNFHIVMERIAPGVVSTTVPLNHLDIWVQVHGLPFGFIQPKVGQGIGSFLGALHTYDSRNTIHSSFMRLKVRIDVTVPLKKEWKVCANNGSYVPVLFKYEKLGVFCYRCGILGHSDKVCPELFELDSDDGVRNWGPDLKPVSTRIGSAANNKWL