AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64130.t1 MTR_3g053920 28.063 253 112 3 71 323 50 232 1.95e-31 119
MS.gene64130.t1 MTR_4g103530 46.316 95 51 0 76 170 1 95 2.08e-27 106
MS.gene64130.t1 MTR_6g033215 34.416 154 92 3 72 219 144 294 2.56e-25 103
MS.gene64130.t1 MTR_2g061500 50.000 80 40 0 71 150 50 129 3.37e-25 99.0
MS.gene64130.t1 MTR_0010s0370 32.680 153 96 3 49 198 29 177 1.89e-23 95.9
MS.gene64130.t1 MTR_0010s0350 32.680 153 96 3 49 198 29 177 1.89e-23 95.9
MS.gene64130.t1 MTR_7g011710 43.529 85 48 0 76 160 1 85 1.97e-19 84.0
MS.gene64130.t1 MTR_6g053230 28.571 140 62 2 63 200 59 162 4.90e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64130.t1 AT3G31430 26.829 164 111 5 76 235 153 311 1.20e-13 71.2

Find 99 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACCACTTGCTTGCCTTGC+TGG 0.221766 5.1:+49933881 None:intergenic
TGTTGGATAAACTGGAAATT+TGG 0.257484 5.1:-49934259 MS.gene64130:CDS
TTCTCTGAAATCTGATTATA+AGG 0.260409 5.1:-49933913 MS.gene64130:CDS
ATCTTCGCACGCACCAGTTT+TGG 0.292636 5.1:+49935683 None:intergenic
TACTTCCTTCATCATTTCCT+TGG 0.298928 5.1:+49933851 None:intergenic
CAGATTCATCTCCTTCTTCA+AGG 0.325963 5.1:+49934085 None:intergenic
GAAATTTGGGTCCAGGTTCA+TGG 0.350593 5.1:-49934245 MS.gene64130:CDS
CATACGCTCCTCCAAGTATT+TGG 0.354821 5.1:+49934463 None:intergenic
GGAGGTGCTGTTGGATAAAC+TGG 0.355392 5.1:-49934267 MS.gene64130:CDS
ACAGGTTCCTGTTTCAGTTC+GGG 0.356496 5.1:-49934388 MS.gene64130:CDS
CAGGTTCATGGGTTGACGTT+TGG 0.358741 5.1:-49934233 MS.gene64130:CDS
AGCAGTATTCTCTTGTGAAT+TGG 0.362067 5.1:+49933773 None:intergenic
CCTTGTGCCCGAACTGAAAC+AGG 0.367990 5.1:+49934381 None:intergenic
ACTTCCTTCATCATTTCCTT+GGG 0.380391 5.1:+49933852 None:intergenic
CACCTCCTTCGCGACCTCTC+CGG 0.385065 5.1:+49934283 None:intergenic
AACAGGTTCCTGTTTCAGTT+CGG 0.393771 5.1:-49934389 MS.gene64130:CDS
TGCAGACAGAGTGATCAATA+AGG 0.403522 5.1:-49934354 MS.gene64130:CDS
TAATCCCTGTGGTGCAGAAC+AGG 0.409306 5.1:-49934406 MS.gene64130:CDS
CACAACAAAGGAGCCAAAAC+TGG 0.410814 5.1:-49935696 MS.gene64130:intron
GTTGGATAAACTGGAAATTT+GGG 0.429731 5.1:-49934258 MS.gene64130:CDS
ACCTCCTTCGCGACCTCTCC+GGG 0.432343 5.1:+49934284 None:intergenic
GGTGCTGGGAGCAGCGCTAA+TGG 0.445071 5.1:-49933801 MS.gene64130:CDS
GTGAATATGATTCGAACAAC+TGG 0.449261 5.1:-49934157 MS.gene64130:CDS
CCTCTCCGGGTGATATCCTC+TGG 0.452654 5.1:+49934297 None:intergenic
TGCTACAGGTGTGGCCTCCT+AGG 0.460206 5.1:-49934005 MS.gene64130:CDS
AAATTTGGGTCCAGGTTCAT+GGG 0.462350 5.1:-49934244 MS.gene64130:CDS
CAACTCTGGAGGCCAGGGAG+GGG 0.463141 5.1:-49934437 MS.gene64130:CDS
ATGGAACAACTCTGGAGGCC+AGG 0.468093 5.1:-49934443 MS.gene64130:CDS
AGACTGTTGATGATGGTGTT+AGG 0.469684 5.1:-49933947 MS.gene64130:CDS
AACTCTTGTCTGTGTGTCCT+AGG 0.473283 5.1:+49933988 None:intergenic
AGGAACCTGTTCTGCACCAC+AGG 0.473306 5.1:+49934401 None:intergenic
TGTTGCTATGGTGGAATCAT+TGG 0.479740 5.1:-49935750 MS.gene64130:CDS
ACGGGATGCGGTGCTTTCCT+CGG 0.482557 5.1:-49934188 MS.gene64130:CDS
AGATTCATCTCCTTCTTCAA+GGG 0.484154 5.1:+49934086 None:intergenic
TGGAGCTGATTCCACTGATA+AGG 0.488659 5.1:-49935831 MS.gene64130:CDS
CAAATACTTGGAGGAGCGTA+TGG 0.491929 5.1:-49934462 MS.gene64130:CDS
TTGTTGTTTGTATCAGACAT+AGG 0.492713 5.1:+49933747 None:intergenic
CAAGGCAAGCAAGTGGTTCA+AGG 0.497986 5.1:-49933877 MS.gene64130:CDS
GAAAAGACCGGCACGGGATG+CGG 0.500244 5.1:-49934200 MS.gene64130:CDS
TAAACTGGAAATTTGGGTCC+AGG 0.502089 5.1:-49934252 MS.gene64130:CDS
AGTACAGAGAATGATGGTGC+TGG 0.503976 5.1:-49933816 MS.gene64130:CDS
CTGGAGGCCAGGGAGGGGAT+TGG 0.506174 5.1:-49934432 MS.gene64130:CDS
CCTGTTTCAGTTCGGGCACA+AGG 0.506981 5.1:-49934381 MS.gene64130:CDS
CTCTTCCAAGGTTTGCTAGT+CGG 0.507200 5.1:+49935776 None:intergenic
CAAACGTCAACCCATGAACC+TGG 0.514645 5.1:+49934234 None:intergenic
ATTAGTAATCAGCTGGTTAG+TGG 0.516547 5.1:-49933720 MS.gene64130:CDS
AAAGATGGTTGTTTGTGAGC+TGG 0.516700 5.1:-49935976 None:intergenic
ACAAATCAAGACCAAATACT+TGG 0.517383 5.1:-49934474 MS.gene64130:intron
GCAGACAGAGTGATCAATAA+GGG 0.532162 5.1:-49934353 MS.gene64130:CDS
GGTCGCGAAGGAGGTGCTGT+TGG 0.534632 5.1:-49934276 MS.gene64130:CDS
CAATGAAATTAGTAATCAGC+TGG 0.539760 5.1:-49933727 MS.gene64130:CDS
GTACAAGAAAAGACCGGCAC+GGG 0.542937 5.1:-49934206 MS.gene64130:CDS
TGTCAATAAGCCCTTGAAGA+AGG 0.544821 5.1:-49934096 MS.gene64130:CDS
GAGGCCACACCTGTAGCAAA+AGG 0.545505 5.1:+49934010 None:intergenic
ACAACTCTGGAGGCCAGGGA+GGG 0.545541 5.1:-49934438 MS.gene64130:CDS
GAAAGCACCGCATCCCGTGC+CGG 0.548699 5.1:+49934193 None:intergenic
TGAATCTGACAACAAACAAA+GGG 0.552040 5.1:-49934070 MS.gene64130:CDS
TGGAACAACTCTGGAGGCCA+GGG 0.552410 5.1:-49934442 MS.gene64130:CDS
ATTACAACCAATCCCCTCCC+TGG 0.554286 5.1:+49934425 None:intergenic
GGATCCCAAGGAAATGATGA+AGG 0.555424 5.1:-49933856 MS.gene64130:CDS
GAAGAGAGCTGTTGCTATGG+TGG 0.556188 5.1:-49935759 MS.gene64130:CDS
TATGATGAGACTGTTGATGA+TGG 0.561706 5.1:-49933954 MS.gene64130:CDS
ATGAATCTGACAACAAACAA+AGG 0.568860 5.1:-49934071 MS.gene64130:CDS
ATAGCAACAGCTCTCTTCCA+AGG 0.569980 5.1:+49935764 None:intergenic
TGAAGGAAGTAGAAGATGTG+AGG 0.572188 5.1:-49933839 MS.gene64130:CDS
TTGATGATGGTGTTAGGATG+TGG 0.574720 5.1:-49933941 MS.gene64130:CDS
TATCAGTGGAATCAGCTCCA+TGG 0.576037 5.1:+49935834 None:intergenic
CAAGTATATGCGGCTAAGAG+TGG 0.576759 5.1:-49934126 MS.gene64130:CDS
TTGGAAGAGAGCTGTTGCTA+TGG 0.584517 5.1:-49935762 MS.gene64130:CDS
GTACAGAGAATGATGGTGCT+GGG 0.585703 5.1:-49933815 MS.gene64130:CDS
AATCAAGACCAAATACTTGG+AGG 0.587075 5.1:-49934471 MS.gene64130:intron
ACCCGGAGAGGTCGCGAAGG+AGG 0.587247 5.1:-49934285 MS.gene64130:CDS
TGACTCCGACTAGCAAACCT+TGG 0.588022 5.1:-49935781 MS.gene64130:CDS
AGCGACATCGTCCTTATCAG+TGG 0.603044 5.1:+49935820 None:intergenic
CAAGTGGTTCAAGGATCCCA+AGG 0.608422 5.1:-49933868 MS.gene64130:CDS
AACAACTCTGGAGGCCAGGG+AGG 0.615709 5.1:-49934439 MS.gene64130:CDS
GAGTGATCAATAAGGGACCT+TGG 0.617924 5.1:-49934346 MS.gene64130:CDS
AGGAGCGTATGGAACAACTC+TGG 0.621259 5.1:-49934451 MS.gene64130:CDS
TATCCTCTGGATAATGATGT+TGG 0.622040 5.1:+49934310 None:intergenic
ATCACCCGGAGAGGTCGCGA+AGG 0.626286 5.1:-49934288 MS.gene64130:CDS
TCTTGTCTGTGTGTCCTAGG+AGG 0.629023 5.1:+49933991 None:intergenic
TAATCAGCTGGTTAGTGGTG+CGG 0.632118 5.1:-49933715 MS.gene64130:CDS
ATTTGATGCATCCACAACAA+AGG 0.644583 5.1:-49935708 MS.gene64130:CDS
TAAGGAAAGTAATTCCAGCA+AGG 0.651257 5.1:-49933895 MS.gene64130:CDS
GGGATTGGTTGTAATCCCTG+TGG 0.659614 5.1:-49934417 MS.gene64130:CDS
GGATACGTACAAGAAAAGAC+CGG 0.664938 5.1:-49934212 MS.gene64130:CDS
GTGAGGAGTACAGAGAATGA+TGG 0.666324 5.1:-49933822 MS.gene64130:CDS
GGAACCTGTTCTGCACCACA+GGG 0.669294 5.1:+49934402 None:intergenic
ATTCCAGCAAGGCAAGCAAG+TGG 0.676219 5.1:-49933884 MS.gene64130:CDS
GGATTCATAGCAAGTATATG+CGG 0.680560 5.1:-49934136 MS.gene64130:CDS
AGCGTATGGAACAACTCTGG+AGG 0.683734 5.1:-49934448 MS.gene64130:CDS
CGTACAAGAAAAGACCGGCA+CGG 0.687867 5.1:-49934207 MS.gene64130:CDS
CCAGAGGATATCACCCGGAG+AGG 0.688028 5.1:-49934297 MS.gene64130:CDS
CATCAAATTCATCAACAGAG+AGG 0.689354 5.1:+49935723 None:intergenic
ATTATCCAGAGGATATCACC+CGG 0.696253 5.1:-49934302 MS.gene64130:CDS
TTGGCGTTGTCATATGACCA+AGG 0.702412 5.1:+49934329 None:intergenic
GCTGGATGATCAAAATTCAG+CGG 0.718534 5.1:-49935958 MS.gene64130:intron
ACGCCAACATCATTATCCAG+AGG 0.725127 5.1:-49934313 MS.gene64130:CDS
CATATTCACGCAGTACACCG+AGG 0.769060 5.1:+49934171 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAAGTATGAAAAATT+AGG - chr5.1:49935638-49935657 MS.gene64130:intron 10.0%
!!! ACTTATGTTACTTATATTTA+TGG - chr5.1:49934155-49934174 MS.gene64130:CDS 15.0%
!!! ATTTCATATTTAAAACAACA+CGG + chr5.1:49934377-49934396 None:intergenic 15.0%
!!! GATTTTTTCCTAAAAAAAAA+TGG + chr5.1:49933820-49933839 None:intergenic 15.0%
!!! TTGCAATTCTATTCTAAAAA+AGG + chr5.1:49934551-49934570 None:intergenic 20.0%
!!! TTTTTTTAGGAAAAAATCCA+TGG - chr5.1:49933822-49933841 MS.gene64130:CDS 20.0%
! AAGTAACAACATAATAAGAC+TGG - chr5.1:49934725-49934744 MS.gene64130:intron 25.0%
! ACAAAATGAAAGAAAGAGAA+GGG + chr5.1:49935049-49935068 None:intergenic 25.0%
! AGTAACAACATAATAAGACT+GGG - chr5.1:49934726-49934745 MS.gene64130:intron 25.0%
! GTATCAATTGAAAAGTCATT+GGG - chr5.1:49934330-49934349 MS.gene64130:CDS 25.0%
! TATTAACATGCACATAATCT+TGG + chr5.1:49934690-49934709 None:intergenic 25.0%
! TGTATCAATTGAAAAGTCAT+TGG - chr5.1:49934329-49934348 MS.gene64130:CDS 25.0%
!! ACAGTCTCATCATAAATTTT+AGG + chr5.1:49935713-49935732 None:intergenic 25.0%
!! ATTCTGGAAAATTGTTTGAT+TGG - chr5.1:49935158-49935177 MS.gene64130:intron 25.0%
!!! TAGAAGTTTTAGCCTATTTA+TGG - chr5.1:49934903-49934922 MS.gene64130:intron 25.0%
!!! TTCTCTGAAATCTGATTATA+AGG - chr5.1:49935760-49935779 MS.gene64130:CDS 25.0%
AAGATTGAATCCAAAGATTG+AGG - chr5.1:49934008-49934027 MS.gene64130:CDS 30.0%
ACAAATCAAGACCAAATACT+TGG - chr5.1:49935199-49935218 MS.gene64130:intron 30.0%
ACCTGATGAAATAAATAGTC+TGG - chr5.1:49934573-49934592 MS.gene64130:intron 30.0%
ATGAATCTGACAACAAACAA+AGG - chr5.1:49935602-49935621 MS.gene64130:intron 30.0%
ATGTTCAGCTCATAATCATA+AGG + chr5.1:49934287-49934306 None:intergenic 30.0%
CAACATTTAAGTCAATTGTC+TGG + chr5.1:49935125-49935144 None:intergenic 30.0%
CAATGAAATTAGTAATCAGC+TGG - chr5.1:49935946-49935965 MS.gene64130:intron 30.0%
GACAAAATGAAAGAAAGAGA+AGG + chr5.1:49935050-49935069 None:intergenic 30.0%
GTTGGATAAACTGGAAATTT+GGG - chr5.1:49935415-49935434 MS.gene64130:intron 30.0%
TCCAGACTATTTATTTCATC+AGG + chr5.1:49934577-49934596 None:intergenic 30.0%
TGAATCTGACAACAAACAAA+GGG - chr5.1:49935603-49935622 MS.gene64130:intron 30.0%
TGTTGGATAAACTGGAAATT+TGG - chr5.1:49935414-49935433 MS.gene64130:intron 30.0%
TTGTAAAAGACCTCAATCTT+TGG + chr5.1:49934021-49934040 None:intergenic 30.0%
! AAATGAAAACGAAAGTGCAA+CGG + chr5.1:49934081-49934100 None:intergenic 30.0%
! AACGAATTTTGCATAGATGT+TGG + chr5.1:49934988-49935007 None:intergenic 30.0%
! ACGAATTTTGCATAGATGTT+GGG + chr5.1:49934987-49935006 None:intergenic 30.0%
!! TTGTTGTTTGTATCAGACAT+AGG + chr5.1:49935929-49935948 None:intergenic 30.0%
!!! GGAATGCACCATTTTTTTTT+AGG - chr5.1:49933809-49933828 MS.gene64130:CDS 30.0%
AAATGGTGCATTCCTTTAGA+TGG + chr5.1:49933803-49933822 None:intergenic 35.0%
AAGAGAAAAGTCAACGTAGT+TGG + chr5.1:49934779-49934798 None:intergenic 35.0%
AATCAAGACCAAATACTTGG+AGG - chr5.1:49935202-49935221 MS.gene64130:intron 35.0%
ACGCTTAAACATTGTCTTCA+AGG - chr5.1:49934452-49934471 MS.gene64130:CDS 35.0%
ACTTCCTTCATCATTTCCTT+GGG + chr5.1:49935824-49935843 None:intergenic 35.0%
AGATTCATCTCCTTCTTCAA+GGG + chr5.1:49935590-49935609 None:intergenic 35.0%
AGCAGTATTCTCTTGTGAAT+TGG + chr5.1:49935903-49935922 None:intergenic 35.0%
ATAATCTTGGCAGTAGAACA+CGG + chr5.1:49934677-49934696 None:intergenic 35.0%
ATCATGGATCTCAACATTGA+AGG - chr5.1:49935078-49935097 MS.gene64130:intron 35.0%
ATTAGTAATCAGCTGGTTAG+TGG - chr5.1:49935953-49935972 MS.gene64130:intron 35.0%
ATTTGATGCATCCACAACAA+AGG - chr5.1:49933965-49933984 MS.gene64130:CDS 35.0%
CATCAAATTCATCAACAGAG+AGG + chr5.1:49933953-49933972 None:intergenic 35.0%
CTGAACATGTATATTGAGCA+TGG - chr5.1:49934205-49934224 MS.gene64130:CDS 35.0%
GGATTCATAGCAAGTATATG+CGG - chr5.1:49935537-49935556 MS.gene64130:intron 35.0%
GGTTACGGAATATCCAAATT+GGG - chr5.1:49934803-49934822 MS.gene64130:intron 35.0%
GTGAATATGATTCGAACAAC+TGG - chr5.1:49935516-49935535 MS.gene64130:intron 35.0%
TAAGGAAAGTAATTCCAGCA+AGG - chr5.1:49935778-49935797 MS.gene64130:CDS 35.0%
TACATGTTCAGAACTTTCCA+TGG + chr5.1:49934196-49934215 None:intergenic 35.0%
TACTTCCTTCATCATTTCCT+TGG + chr5.1:49935825-49935844 None:intergenic 35.0%
TATCCTCTGGATAATGATGT+TGG + chr5.1:49935366-49935385 None:intergenic 35.0%
TCCAAATCAGACAGAAAATC+AGG + chr5.1:49934866-49934885 None:intergenic 35.0%
TGAATGACACTGCCATAAAT+AGG + chr5.1:49934918-49934937 None:intergenic 35.0%
TGCATGTTAATAAGATTGCG+AGG - chr5.1:49934698-49934717 MS.gene64130:intron 35.0%
TTGATGTTGCTGCTATATTC+TGG - chr5.1:49935142-49935161 MS.gene64130:intron 35.0%
TTTCCATTGTGAATATGCGA+AGG - chr5.1:49934427-49934446 MS.gene64130:CDS 35.0%
! AATTAGGTACCTTTTGCTAC+AGG - chr5.1:49935654-49935673 MS.gene64130:intron 35.0%
! AGGTCTTTTACAAGATTGTG+GGG - chr5.1:49934028-49934047 MS.gene64130:CDS 35.0%
! ATTTTGTCTTGCTGCTATCA+TGG - chr5.1:49935062-49935081 MS.gene64130:intron 35.0%
! CGAATTTTGCATAGATGTTG+GGG + chr5.1:49934986-49935005 None:intergenic 35.0%
! GAGGTCTTTTACAAGATTGT+GGG - chr5.1:49934027-49934046 MS.gene64130:CDS 35.0%
! TATGATGAGACTGTTGATGA+TGG - chr5.1:49935719-49935738 MS.gene64130:CDS 35.0%
! TGAGGTCTTTTACAAGATTG+TGG - chr5.1:49934026-49934045 MS.gene64130:CDS 35.0%
!!! AAGAATTTTGGTAATGGAGG+TGG + chr5.1:49935022-49935041 None:intergenic 35.0%
!!! ACGTTGACTTTTCTCTTCTA+GGG - chr5.1:49934782-49934801 MS.gene64130:intron 35.0%
!!! ACTTTTCTCTTCTAGGGTTA+CGG - chr5.1:49934788-49934807 MS.gene64130:intron 35.0%
!!! GACAAGAATTTTGGTAATGG+AGG + chr5.1:49935025-49935044 None:intergenic 35.0%
!!! TACGTTGACTTTTCTCTTCT+AGG - chr5.1:49934781-49934800 MS.gene64130:intron 35.0%
AAATTTGGGTCCAGGTTCAT+GGG - chr5.1:49935429-49935448 MS.gene64130:intron 40.0%
AACAGGTTCCTGTTTCAGTT+CGG - chr5.1:49935284-49935303 MS.gene64130:intron 40.0%
ACATTGTCTTCAAGGCAAGA+TGG - chr5.1:49934460-49934479 MS.gene64130:CDS 40.0%
AGAAAATCAGGCTCCAACAA+GGG + chr5.1:49934854-49934873 None:intergenic 40.0%
ATTATCCAGAGGATATCACC+CGG - chr5.1:49935371-49935390 MS.gene64130:intron 40.0%
CAGATTCATCTCCTTCTTCA+AGG + chr5.1:49935591-49935610 None:intergenic 40.0%
GCAGACAGAGTGATCAATAA+GGG - chr5.1:49935320-49935339 MS.gene64130:intron 40.0%
GCTGGATGATCAAAATTCAG+CGG - chr5.1:49933715-49933734 MS.gene64130:CDS 40.0%
GGATACGTACAAGAAAAGAC+CGG - chr5.1:49935461-49935480 MS.gene64130:intron 40.0%
GGCTTAACCATTCAAGAAGA+TGG - chr5.1:49935099-49935118 MS.gene64130:intron 40.0%
GGGTTACGGAATATCCAAAT+TGG - chr5.1:49934802-49934821 MS.gene64130:intron 40.0%
TAAACTGGAAATTTGGGTCC+AGG - chr5.1:49935421-49935440 MS.gene64130:intron 40.0%
TAAGACTGGGATTATACGAG+TGG - chr5.1:49934739-49934758 MS.gene64130:intron 40.0%
TCTCCTTCGCATATTCACAA+TGG + chr5.1:49934433-49934452 None:intergenic 40.0%
TGAAGGAAGTAGAAGATGTG+AGG - chr5.1:49935834-49935853 MS.gene64130:CDS 40.0%
TGCAGACAGAGTGATCAATA+AGG - chr5.1:49935319-49935338 MS.gene64130:intron 40.0%
TGTCAATAAGCCCTTGAAGA+AGG - chr5.1:49935577-49935596 MS.gene64130:intron 40.0%
! GCCTGATTTTCTGTCTGATT+TGG - chr5.1:49934862-49934881 MS.gene64130:intron 40.0%
! TGCATAGATGTTGGGGTTTT+TGG + chr5.1:49934979-49934998 None:intergenic 40.0%
! TGTTGCTATGGTGGAATCAT+TGG - chr5.1:49933923-49933942 MS.gene64130:CDS 40.0%
!! AGACTGTTGATGATGGTGTT+AGG - chr5.1:49935726-49935745 MS.gene64130:CDS 40.0%
!! GGCGACAAGAATTTTGGTAA+TGG + chr5.1:49935028-49935047 None:intergenic 40.0%
!! TGATTTGGATTTTCCAGGTC+TGG - chr5.1:49934877-49934896 MS.gene64130:intron 40.0%
!! TGCGTTTTCTGAAGAATCTG+TGG + chr5.1:49934948-49934967 None:intergenic 40.0%
!! TGGTAATGGAGGTGGAATTT+GGG + chr5.1:49935014-49935033 None:intergenic 40.0%
!! TTGATGATGGTGTTAGGATG+TGG - chr5.1:49935732-49935751 MS.gene64130:CDS 40.0%
!! TTGGTAATGGAGGTGGAATT+TGG + chr5.1:49935015-49935034 None:intergenic 40.0%
!!! CTGTCTGATTTGGATTTTCC+AGG - chr5.1:49934872-49934891 MS.gene64130:intron 40.0%
AAAACTTCTACGTCCAGACC+TGG + chr5.1:49934893-49934912 None:intergenic 45.0%
AACTCTTGTCTGTGTGTCCT+AGG + chr5.1:49935688-49935707 None:intergenic 45.0%
ACAGGTTCCTGTTTCAGTTC+GGG - chr5.1:49935285-49935304 MS.gene64130:intron 45.0%
ACGCCAACATCATTATCCAG+AGG - chr5.1:49935360-49935379 MS.gene64130:intron 45.0%
ATAGCAACAGCTCTCTTCCA+AGG + chr5.1:49933912-49933931 None:intergenic 45.0%
CAAGTATATGCGGCTAAGAG+TGG - chr5.1:49935547-49935566 MS.gene64130:intron 45.0%
CACAACAAAGGAGCCAAAAC+TGG - chr5.1:49933977-49933996 MS.gene64130:CDS 45.0%
CAGAAAATCAGGCTCCAACA+AGG + chr5.1:49934855-49934874 None:intergenic 45.0%
CATACGCTCCTCCAAGTATT+TGG + chr5.1:49935213-49935232 None:intergenic 45.0%
CTGAACATGTCTATCGAGCA+TGG - chr5.1:49934299-49934318 MS.gene64130:CDS 45.0%
GAAATTTGGGTCCAGGTTCA+TGG - chr5.1:49935428-49935447 MS.gene64130:intron 45.0%
GAGTAGCAGCAACCATCTAA+AGG - chr5.1:49933788-49933807 MS.gene64130:CDS 45.0%
GAGTGATCAATAAGGGACCT+TGG - chr5.1:49935327-49935346 MS.gene64130:intron 45.0%
GGATCCCAAGGAAATGATGA+AGG - chr5.1:49935817-49935836 MS.gene64130:CDS 45.0%
TAATCAGCTGGTTAGTGGTG+CGG - chr5.1:49935958-49935977 MS.gene64130:intron 45.0%
TATCAGTGGAATCAGCTCCA+TGG + chr5.1:49933842-49933861 None:intergenic 45.0%
TGTCTGGCCATCTTCTTGAA+TGG + chr5.1:49935109-49935128 None:intergenic 45.0%
TTGGAAGAGAGCTGTTGCTA+TGG - chr5.1:49933911-49933930 MS.gene64130:CDS 45.0%
TTGGCGTTGTCATATGACCA+AGG + chr5.1:49935347-49935366 None:intergenic 45.0%
! AAACGAAAGTGCAACGGACT+TGG + chr5.1:49934075-49934094 None:intergenic 45.0%
! AGTACAGAGAATGATGGTGC+TGG - chr5.1:49935857-49935876 MS.gene64130:intron 45.0%
! CAAATACTTGGAGGAGCGTA+TGG - chr5.1:49935211-49935230 MS.gene64130:intron 45.0%
! GAGAAGGGCGACAAGAATTT+TGG + chr5.1:49935034-49935053 None:intergenic 45.0%
! GGTGTTGTGTGTATTGACCA+TGG - chr5.1:49934176-49934195 MS.gene64130:CDS 45.0%
! GTAATGGAGGTGGAATTTGG+GGG + chr5.1:49935012-49935031 None:intergenic 45.0%
! GTACAGAGAATGATGGTGCT+GGG - chr5.1:49935858-49935877 MS.gene64130:intron 45.0%
! GTGAGGAGTACAGAGAATGA+TGG - chr5.1:49935851-49935870 MS.gene64130:intron 45.0%
! TGGAGCTGATTCCACTGATA+AGG - chr5.1:49933842-49933861 MS.gene64130:CDS 45.0%
!! CTCTTCCAAGGTTTGCTAGT+CGG + chr5.1:49933900-49933919 None:intergenic 45.0%
!! GGTAATGGAGGTGGAATTTG+GGG + chr5.1:49935013-49935032 None:intergenic 45.0%
!!! CAGTTTTGGCTCCTTTGTTG+TGG + chr5.1:49933979-49933998 None:intergenic 45.0%
AAATCAGGCTCCAACAAGGG+CGG + chr5.1:49934851-49934870 None:intergenic 50.0%
AGCGACATCGTCCTTATCAG+TGG + chr5.1:49933856-49933875 None:intergenic 50.0%
AGCGTATGGAACAACTCTGG+AGG - chr5.1:49935225-49935244 MS.gene64130:intron 50.0%
ATTACAACCAATCCCCTCCC+TGG + chr5.1:49935251-49935270 None:intergenic 50.0%
ATTCCAGCAAGGCAAGCAAG+TGG - chr5.1:49935789-49935808 MS.gene64130:CDS 50.0%
CAAACGTCAACCCATGAACC+TGG + chr5.1:49935442-49935461 None:intergenic 50.0%
CAAGGCAAGCAAGTGGTTCA+AGG - chr5.1:49935796-49935815 MS.gene64130:CDS 50.0%
CAAGTGGTTCAAGGATCCCA+AGG - chr5.1:49935805-49935824 MS.gene64130:CDS 50.0%
CATATTCACGCAGTACACCG+AGG + chr5.1:49935505-49935524 None:intergenic 50.0%
CGTACAAGAAAAGACCGGCA+CGG - chr5.1:49935466-49935485 MS.gene64130:intron 50.0%
GAAGAGAGCTGTTGCTATGG+TGG - chr5.1:49933914-49933933 MS.gene64130:CDS 50.0%
GTACAAGAAAAGACCGGCAC+GGG - chr5.1:49935467-49935486 MS.gene64130:intron 50.0%
TAATCCCTGTGGTGCAGAAC+AGG - chr5.1:49935267-49935286 MS.gene64130:intron 50.0%
TCTTGTCTGTGTGTCCTAGG+AGG + chr5.1:49935685-49935704 None:intergenic 50.0%
TGACTCCGACTAGCAAACCT+TGG - chr5.1:49933892-49933911 MS.gene64130:CDS 50.0%
! AGGAGCGTATGGAACAACTC+TGG - chr5.1:49935222-49935241 MS.gene64130:intron 50.0%
! ATCTTCGCACGCACCAGTTT+TGG + chr5.1:49933993-49934012 None:intergenic 50.0%
! CAGGTTCATGGGTTGACGTT+TGG - chr5.1:49935440-49935459 MS.gene64130:intron 50.0%
! GGTACCTTTTGCTACAGGTG+TGG - chr5.1:49935659-49935678 MS.gene64130:intron 50.0%
!! GGAGGTGCTGTTGGATAAAC+TGG - chr5.1:49935406-49935425 MS.gene64130:intron 50.0%
!! GGGATTGGTTGTAATCCCTG+TGG - chr5.1:49935256-49935275 MS.gene64130:intron 50.0%
AGGAACCTGTTCTGCACCAC+AGG + chr5.1:49935275-49935294 None:intergenic 55.0%
CCTGTTTCAGTTCGGGCACA+AGG - chr5.1:49935292-49935311 MS.gene64130:intron 55.0%
CCTTGTGCCCGAACTGAAAC+AGG + chr5.1:49935295-49935314 None:intergenic 55.0%
CTGAAAACGGCGGCCCAATT+TGG + chr5.1:49934819-49934838 None:intergenic 55.0%
GAACCACTTGCTTGCCTTGC+TGG + chr5.1:49935795-49935814 None:intergenic 55.0%
GAGGCCACACCTGTAGCAAA+AGG + chr5.1:49935666-49935685 None:intergenic 55.0%
GCGATACCCACTGAAAACGG+CGG + chr5.1:49934829-49934848 None:intergenic 55.0%
GCGGCGATACCCACTGAAAA+CGG + chr5.1:49934832-49934851 None:intergenic 55.0%
GGAACCTGTTCTGCACCACA+GGG + chr5.1:49935274-49935293 None:intergenic 55.0%
! ATGGAACAACTCTGGAGGCC+AGG - chr5.1:49935230-49935249 MS.gene64130:intron 55.0%
! TGGAACAACTCTGGAGGCCA+GGG - chr5.1:49935231-49935250 MS.gene64130:intron 55.0%
!! AATTGGGCCGCCGTTTTCAG+TGG - chr5.1:49934819-49934838 MS.gene64130:intron 55.0%
!! ATTGGGCCGCCGTTTTCAGT+GGG - chr5.1:49934820-49934839 MS.gene64130:intron 55.0%
ACGGGATGCGGTGCTTTCCT+CGG - chr5.1:49935485-49935504 MS.gene64130:intron 60.0%
CCAGAGGATATCACCCGGAG+AGG - chr5.1:49935376-49935395 MS.gene64130:intron 60.0%
CCTCTCCGGGTGATATCCTC+TGG + chr5.1:49935379-49935398 None:intergenic 60.0%
GAAAAGACCGGCACGGGATG+CGG - chr5.1:49935473-49935492 MS.gene64130:intron 60.0%
TGCTACAGGTGTGGCCTCCT+AGG - chr5.1:49935668-49935687 MS.gene64130:intron 60.0%
! AACAACTCTGGAGGCCAGGG+AGG - chr5.1:49935234-49935253 MS.gene64130:intron 60.0%
! ACAACTCTGGAGGCCAGGGA+GGG - chr5.1:49935235-49935254 MS.gene64130:intron 60.0%
ACCTCCTTCGCGACCTCTCC+GGG + chr5.1:49935392-49935411 None:intergenic 65.0%
ATCACCCGGAGAGGTCGCGA+AGG - chr5.1:49935385-49935404 MS.gene64130:intron 65.0%
CACCTCCTTCGCGACCTCTC+CGG + chr5.1:49935393-49935412 None:intergenic 65.0%
! CAACTCTGGAGGCCAGGGAG+GGG - chr5.1:49935236-49935255 MS.gene64130:intron 65.0%
! GGTGCTGGGAGCAGCGCTAA+TGG - chr5.1:49935872-49935891 MS.gene64130:intron 65.0%
! GTGGGTATCGCCGCCCTTGT+TGG - chr5.1:49934838-49934857 MS.gene64130:intron 65.0%
!! GAAAGCACCGCATCCCGTGC+CGG + chr5.1:49935483-49935502 None:intergenic 65.0%
!! GGTCGCGAAGGAGGTGCTGT+TGG - chr5.1:49935397-49935416 MS.gene64130:intron 65.0%
ACCCGGAGAGGTCGCGAAGG+AGG - chr5.1:49935388-49935407 MS.gene64130:intron 70.0%
CTGGAGGCCAGGGAGGGGAT+TGG - chr5.1:49935241-49935260 MS.gene64130:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 49933701 49935994 49933701 ID=MS.gene64130
chr5.1 mRNA 49933701 49935994 49933701 ID=MS.gene64130.t1;Parent=MS.gene64130
chr5.1 exon 49935959 49935994 49935959 ID=MS.gene64130.t1.exon1;Parent=MS.gene64130.t1
chr5.1 CDS 49935959 49935994 49935959 ID=cds.MS.gene64130.t1;Parent=MS.gene64130.t1
chr5.1 exon 49935697 49935864 49935697 ID=MS.gene64130.t1.exon2;Parent=MS.gene64130.t1
chr5.1 CDS 49935697 49935864 49935697 ID=cds.MS.gene64130.t1;Parent=MS.gene64130.t1
chr5.1 exon 49933701 49934486 49933701 ID=MS.gene64130.t1.exon3;Parent=MS.gene64130.t1
chr5.1 CDS 49933701 49934486 49933701 ID=cds.MS.gene64130.t1;Parent=MS.gene64130.t1
Gene Sequence

>MS.gene64130

ATGGTTGTTTGTGAGCTGGATGATCAAAATTCAGCGGTATATATTTATATCAAATTAGATCACATTATTTTGATTTGTTAAAGAATTGAGTAGCAGCAACCATCTAAAGGAATGCACCATTTTTTTTTAGGAAAAAATCCATGGAGCTGATTCCACTGATAAGGACGATGTCGCTATAGTTGATTCAATTGTGACTCCGACTAGCAAACCTTGGAAGAGAGCTGTTGCTATGGTGGAATCATTGGATGACCTCTCTGTTGATGAATTTGATGCATCCACAACAAAGGAGCCAAAACTGGTGCGTGCGAAGATTGAATCCAAAGATTGAGGTCTTTTACAAGATTGTGGGGTAGCAGTTCTGTTGCTGATGTCCAAGTCCGTTGCACTTTCGTTTTCATTTGTTTTTTGTTTTTGTTTTGTTGTGTCGCACTTTTAAGTTGTGTTATGTTCTCAGACTTATGTTACTTATATTTATGGTGTTGTGTGTATTGACCATGGAAAGTTCTGAACATGTATATTGAGCATGGAAAGTTCTGAACATGTCTAAGACTTATGTTTTTTGTTTTTGTTTTGTTGTGTCGCACCTTATGATTATGAGCTGAACATGTCTATCGAGCATGGAAAGTTATGTATCAATTGAAAAGTCATTGGGTGTTTTGATCTAATTATCGTTCCGTGTTGTTTTAAATATGAAATTAACAAAAAAAATGCTTAATTATATTTTTATTTCCATTGTGAATATGCGAAGGAGACGCTTAAACATTGTCTTCAAGGCAAGATGGTTACTTATAATATAGTCAGAAACTCAATAGTATAGAGTGCAGAATTTGTTAATTTAAAATTGAATCCTTTTTTAGAATAGAATTGCAAGCACCTGATGAAATAAATAGTCTGGAGAATATTATTGCAAAGATTCTGCTTATTTATATGAATTATGAAGACTTTGAAAAGTTATTATCATTATGTTCTCTTTCCGTGTTCTACTGCCAAGATTATGTGCATGTTAATAAGATTGCGAGGCTGAAAGTAACAACATAATAAGACTGGGATTATACGAGTGGTATACGCAACTTTGCCAACTACGTTGACTTTTCTCTTCTAGGGTTACGGAATATCCAAATTGGGCCGCCGTTTTCAGTGGGTATCGCCGCCCTTGTTGGAGCCTGATTTTCTGTCTGATTTGGATTTTCCAGGTCTGGACGTAGAAGTTTTAGCCTATTTATGGCAGTGTCATTCATTGTATTCCACAGATTCTTCAGAAAACGCAAGATTAAACCAAAAACCCCAACATCTATGCAAAATTCGTTTCCCCCAAATTCCACCTCCATTACCAAAATTCTTGTCGCCCTTCTCTTTCTTTCATTTTGTCTTGCTGCTATCATGGATCTCAACATTGAAGGCTTAACCATTCAAGAAGATGGCCAGACAATTGACTTAAATGTTGATGTTGCTGCTATATTCTGGAAAATTGTTTGATTGGTTGTGTTTTGACTGATAAACAAATCAAGACCAAATACTTGGAGGAGCGTATGGAACAACTCTGGAGGCCAGGGAGGGGATTGGTTGTAATCCCTGTGGTGCAGAACAGGTTCCTGTTTCAGTTCGGGCACAAGGCAGATGCAGACAGAGTGATCAATAAGGGACCTTGGTCATATGACAACGCCAACATCATTATCCAGAGGATATCACCCGGAGAGGTCGCGAAGGAGGTGCTGTTGGATAAACTGGAAATTTGGGTCCAGGTTCATGGGTTGACGTTTGGATACGTACAAGAAAAGACCGGCACGGGATGCGGTGCTTTCCTCGGTGTACTGCGTGAATATGATTCGAACAACTGGATTCATAGCAAGTATATGCGGCTAAGAGTGGCTATAGATGTCAATAAGCCCTTGAAGAAGGAGATGAATCTGACAACAAACAAAGGGAGTTGTGCTGTCTATTTTAAGTATGAAAAATTAGGTACCTTTTGCTACAGGTGTGGCCTCCTAGGACACACAGACAAGAGTTGTCCTAAAATTTATGATGAGACTGTTGATGATGGTGTTAGGATGTGGTCTAATTCTCTGAAATCTGATTATAAGGAAAGTAATTCCAGCAAGGCAAGCAAGTGGTTCAAGGATCCCAAGGAAATGATGAAGGAAGTAGAAGATGTGAGGAGTACAGAGAATGATGGTGCTGGGAGCAGCGCTAATGGCATGTCCAATTCACAAGAGAATACTGCTCAGCCTATGTCTGATACAAACAACAATGAAATTAGTAATCAGCTGGTTAGTGGTGCGGTGCAGCATGATTAA
Protein sequence

>MS.gene64130.t1

MVVCELDDQNSAEKIHGADSTDKDDVAIVDSIVTPTSKPWKRAVAMVESLDDLSVDEFDASTTKEPKLTKYLEERMEQLWRPGRGLVVIPVVQNRFLFQFGHKADADRVINKGPWSYDNANIIIQRISPGEVAKEVLLDKLEIWVQVHGLTFGYVQEKTGTGCGAFLGVLREYDSNNWIHSKYMRLRVAIDVNKPLKKEMNLTTNKGSCAVYFKYEKLGTFCYRCGLLGHTDKSCPKIYDETVDDGVRMWSNSLKSDYKESNSSKASKWFKDPKEMMKEVEDVRSTENDGAGSSANGMSNSQENTAQPMSDTNNNEISNQLVSGAVQHD