AlfalfaGEDB | MS.gene63150

M. sativa cultivar XinJiangDaYe / MS.gene63150

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63150.t1	XP_024630779.1	76.6	239	56	0	1	239	1	239	2.60E-107	398.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63150.t1	A0A396JIW9	72.5	247	68	0	1	247	1	247	2.6e-104	387.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34812	MS.gene63150	PPI
MS.gene02896	MS.gene63150	PPI
MS.gene063214	MS.gene63150	PPI
MS.gene55682	MS.gene63150	PPI
MS.gene97410	MS.gene63150	PPI
MS.gene32415	MS.gene63150	PPI
MS.gene63150	MS.gene40469	PPI
MS.gene009213	MS.gene63150	PPI
MS.gene035626	MS.gene63150	PPI
MS.gene067340	MS.gene63150	PPI
MS.gene63150	MS.gene043140	PPI
MS.gene65623	MS.gene63150	PPI
MS.gene63150	MS.gene03149	PPI
MS.gene71675	MS.gene63150	PPI
MS.gene019177	MS.gene63150	PPI
MS.gene69495	MS.gene63150	PPI
MS.gene049512	MS.gene63150	PPI
MS.gene63150	MS.gene29321	PPI
MS.gene038623	MS.gene63150	PPI
MS.gene55209	MS.gene63150	PPI
MS.gene05357	MS.gene63150	PPI
MS.gene032311	MS.gene63150	PPI
MS.gene64178	MS.gene63150	PPI
MS.gene004417	MS.gene63150	PPI
MS.gene030090	MS.gene63150	PPI
MS.gene96174	MS.gene63150	PPI
MS.gene63150	MS.gene24278	PPI
MS.gene86849	MS.gene63150	PPI
MS.gene50392	MS.gene63150	PPI
MS.gene63150	MS.gene34618	PPI
MS.gene072852	MS.gene63150	PPI
MS.gene63150	MS.gene59460	PPI
MS.gene57019	MS.gene63150	PPI
MS.gene63150	MS.gene81194	PPI
MS.gene63150	MS.gene060773	PPI
MS.gene39956	MS.gene63150	PPI
MS.gene63150	MS.gene08713	PPI
MS.gene63150	MS.gene038394	PPI
MS.gene021968	MS.gene63150	PPI
MS.gene63150	MS.gene019001	PPI
MS.gene09589	MS.gene63150	PPI
MS.gene63150	MS.gene24070	PPI
MS.gene63150	MS.gene73261	PPI
MS.gene43393	MS.gene63150	PPI
MS.gene057828	MS.gene63150	PPI
MS.gene34197	MS.gene63150	PPI
MS.gene63150	MS.gene055546	PPI
MS.gene66650	MS.gene63150	PPI
MS.gene005120	MS.gene63150	PPI
MS.gene63150	MS.gene44144	PPI
MS.gene048072	MS.gene63150	PPI
MS.gene060386	MS.gene63150	PPI
MS.gene05436	MS.gene63150	PPI
MS.gene63150	MS.gene068629	PPI
MS.gene63150	MS.gene004417	PPI
MS.gene059736	MS.gene63150	PPI
MS.gene70932	MS.gene63150	PPI
MS.gene63150	MS.gene009781	PPI
MS.gene35175	MS.gene63150	PPI
MS.gene021965	MS.gene63150	PPI
MS.gene055683	MS.gene63150	PPI
MS.gene042614	MS.gene63150	PPI
MS.gene63150	MS.gene21066	PPI
MS.gene41643	MS.gene63150	PPI
MS.gene63150	MS.gene009213	PPI
MS.gene26844	MS.gene63150	PPI
MS.gene63150	MS.gene98127	PPI
MS.gene021555	MS.gene63150	PPI
MS.gene63150	MS.gene003264	PPI
MS.gene021647	MS.gene63150	PPI
MS.gene96109	MS.gene63150	PPI
MS.gene019165	MS.gene63150	PPI
MS.gene048706	MS.gene63150	PPI
MS.gene009121	MS.gene63150	PPI
MS.gene94654	MS.gene63150	PPI
MS.gene63150	MS.gene49057	PPI
MS.gene63150	MS.gene89815	PPI
MS.gene51804	MS.gene63150	PPI
MS.gene87845	MS.gene63150	PPI
MS.gene36069	MS.gene63150	PPI
MS.gene63150	MS.gene018474	PPI
MS.gene63150	MS.gene048088	PPI
MS.gene63150	MS.gene86868	PPI
MS.gene61452	MS.gene63150	PPI
MS.gene83772	MS.gene63150	PPI
MS.gene63150	MS.gene96163	PPI
MS.gene008927	MS.gene63150	PPI
MS.gene016102	MS.gene63150	PPI
MS.gene46941	MS.gene63150	PPI
MS.gene045614	MS.gene63150	PPI
MS.gene96541	MS.gene63150	PPI
MS.gene049561	MS.gene63150	PPI
MS.gene000634	MS.gene63150	PPI
MS.gene63762	MS.gene63150	PPI
MS.gene30208	MS.gene63150	PPI
MS.gene028716	MS.gene63150	PPI
MS.gene025873	MS.gene63150	PPI
MS.gene012019	MS.gene63150	PPI
MS.gene06164	MS.gene63150	PPI
MS.gene63150	MS.gene012889	PPI
MS.gene63150	MS.gene34463	PPI
MS.gene71588	MS.gene63150	PPI
MS.gene93396	MS.gene63150	PPI
MS.gene013676	MS.gene63150	PPI
MS.gene05358	MS.gene63150	PPI
MS.gene95561	MS.gene63150	PPI
MS.gene63150	MS.gene61499	PPI
MS.gene074257	MS.gene63150	PPI
MS.gene018196	MS.gene63150	PPI
MS.gene065627	MS.gene63150	PPI
MS.gene018745	MS.gene63150	PPI
MS.gene65546	MS.gene63150	PPI
MS.gene059269	MS.gene63150	PPI
MS.gene000506	MS.gene63150	PPI
MS.gene03936	MS.gene63150	PPI
MS.gene038098	MS.gene63150	PPI
MS.gene56991	MS.gene63150	PPI
MS.gene60059	MS.gene63150	PPI
MS.gene63150	MS.gene53904	PPI
MS.gene85538	MS.gene63150	PPI
MS.gene22111	MS.gene63150	PPI
MS.gene28545	MS.gene63150	PPI
MS.gene63150	MS.gene025675	PPI
MS.gene28718	MS.gene63150	PPI
MS.gene055560	MS.gene63150	PPI
MS.gene008275	MS.gene63150	PPI
MS.gene63150	MS.gene55209	PPI
MS.gene038757	MS.gene63150	PPI
MS.gene064966	MS.gene63150	PPI
MS.gene002590	MS.gene63150	PPI
MS.gene63150	MS.gene022929	PPI
MS.gene63150	MS.gene007022	PPI
MS.gene63150	MS.gene52349	PPI
MS.gene40828	MS.gene63150	PPI
MS.gene43924	MS.gene63150	PPI
MS.gene046089	MS.gene63150	PPI
MS.gene047954	MS.gene63150	PPI
MS.gene036423	MS.gene63150	PPI
MS.gene63150	MS.gene048342	PPI
MS.gene63150	MS.gene069624	PPI
MS.gene054652	MS.gene63150	PPI
MS.gene013644	MS.gene63150	PPI
MS.gene63150	MS.gene013890	PPI
MS.gene72520	MS.gene63150	PPI
MS.gene66651	MS.gene63150	PPI
MS.gene63150	MS.gene016719	PPI
MS.gene63150	MS.gene028714	PPI
MS.gene63150	MS.gene88393	PPI
MS.gene35202	MS.gene63150	PPI
MS.gene63150	MS.gene048706	PPI
MS.gene051449	MS.gene63150	PPI
MS.gene63150	MS.gene69586	PPI
MS.gene63150	MS.gene45083	PPI
MS.gene63743	MS.gene63150	PPI
MS.gene63150	MS.gene049561	PPI
MS.gene63150	MS.gene008658	PPI
MS.gene48477	MS.gene63150	PPI
MS.gene045875	MS.gene63150	PPI
MS.gene30136	MS.gene63150	PPI
MS.gene008861	MS.gene63150	PPI
MS.gene63150	MS.gene08708	PPI
MS.gene73261	MS.gene63150	PPI
MS.gene012748	MS.gene63150	PPI
MS.gene024297	MS.gene63150	PPI
MS.gene83979	MS.gene63150	PPI
MS.gene63150	MS.gene06164	PPI
MS.gene85295	MS.gene63150	PPI
MS.gene47792	MS.gene63150	PPI
MS.gene46948	MS.gene63150	PPI
MS.gene98471	MS.gene63150	PPI
MS.gene049610	MS.gene63150	PPI
MS.gene63150	MS.gene000640	PPI
MS.gene060718	MS.gene63150	PPI
MS.gene63150	MS.gene000506	PPI
MS.gene05576	MS.gene63150	PPI
MS.gene63150	MS.gene028716	PPI
MS.gene63150	MS.gene74639	PPI
MS.gene63150	MS.gene071133	PPI
MS.gene63150	MS.gene60266	PPI
MS.gene52349	MS.gene63150	PPI
MS.gene05231	MS.gene63150	PPI
MS.gene63150	MS.gene54051	PPI
MS.gene63150	MS.gene22111	PPI
MS.gene010656	MS.gene63150	PPI
MS.gene000629	MS.gene63150	PPI
MS.gene63150	MS.gene010290	PPI
MS.gene40469	MS.gene63150	PPI
MS.gene041264	MS.gene63150	PPI
MS.gene41979	MS.gene63150	PPI
MS.gene63150	MS.gene37955	PPI
MS.gene024522	MS.gene63150	PPI
MS.gene63150	MS.gene92517	PPI
MS.gene64130	MS.gene63150	PPI
MS.gene05319	MS.gene63150	PPI
MS.gene63150	MS.gene010890	PPI
MS.gene63150	MS.gene84790	PPI
MS.gene050679	MS.gene63150	PPI
MS.gene20246	MS.gene63150	PPI
MS.gene055223	MS.gene63150	PPI
MS.gene016719	MS.gene63150	PPI
MS.gene053747	MS.gene63150	PPI
MS.gene65471	MS.gene63150	PPI
MS.gene63150	MS.gene060788	PPI
MS.gene061850	MS.gene63150	PPI
MS.gene63150	MS.gene77990	PPI
MS.gene63150	MS.gene062511	PPI
MS.gene26433	MS.gene63150	PPI
MS.gene63150	MS.gene027994	PPI
MS.gene22730	MS.gene63150	PPI
MS.gene073582	MS.gene63150	PPI
MS.gene042559	MS.gene63150	PPI
MS.gene058889	MS.gene63150	PPI
MS.gene63150	MS.gene69584	PPI
MS.gene98616	MS.gene63150	PPI
MS.gene63150	MS.gene46353	PPI
MS.gene042027	MS.gene63150	PPI
MS.gene85308	MS.gene63150	PPI
MS.gene69490	MS.gene63150	PPI
MS.gene63150	MS.gene51799	PPI
MS.gene015248	MS.gene63150	PPI
MS.gene63150	MS.gene61651	PPI
MS.gene80362	MS.gene63150	PPI
MS.gene44083	MS.gene63150	PPI
MS.gene013193	MS.gene63150	PPI
MS.gene04970	MS.gene63150	PPI
MS.gene08106	MS.gene63150	PPI
MS.gene51799	MS.gene63150	PPI
MS.gene63150	MS.gene09023	PPI
MS.gene58419	MS.gene63150	PPI
MS.gene63150	MS.gene054508	PPI
MS.gene63150	MS.gene025987	PPI
MS.gene63150	MS.gene95960	PPI
MS.gene63150	MS.gene053373	PPI
MS.gene23521	MS.gene63150	PPI
MS.gene29406	MS.gene63150	PPI
MS.gene86275	MS.gene63150	PPI
MS.gene63150	MS.gene65474	PPI
MS.gene87529	MS.gene63150	PPI
MS.gene024405	MS.gene63150	PPI
MS.gene63150	MS.gene025401	PPI
MS.gene63150	MS.gene015093	PPI
MS.gene04134	MS.gene63150	PPI
MS.gene062067	MS.gene63150	PPI
MS.gene63150	MS.gene50392	PPI
MS.gene63150	MS.gene22730	PPI
MS.gene63150	MS.gene000379	PPI
MS.gene63150	MS.gene032299	PPI
MS.gene57044	MS.gene63150	PPI
MS.gene63150	MS.gene08606	PPI
MS.gene89815	MS.gene63150	PPI
MS.gene028714	MS.gene63150	PPI
MS.gene017586	MS.gene63150	PPI
MS.gene038676	MS.gene63150	PPI
MS.gene63150	MS.gene09517	PPI
MS.gene71637	MS.gene63150	PPI
MS.gene63150	MS.gene98756	PPI
MS.gene63150	MS.gene87845	PPI
MS.gene042651	MS.gene63150	PPI
MS.gene63150	MS.gene62249	PPI
MS.gene98467	MS.gene63150	PPI
MS.gene037049	MS.gene63150	PPI
MS.gene034643	MS.gene63150	PPI
MS.gene037634	MS.gene63150	PPI
MS.gene63150	MS.gene044729	PPI
MS.gene04749	MS.gene63150	PPI
MS.gene63150	MS.gene60390	PPI
MS.gene63150	MS.gene039692	PPI
MS.gene63150	MS.gene027169	PPI
MS.gene071784	MS.gene63150	PPI
MS.gene63150	MS.gene019177	PPI
MS.gene027169	MS.gene63150	PPI
MS.gene061579	MS.gene63150	PPI
MS.gene76420	MS.gene63150	PPI
MS.gene071320	MS.gene63150	PPI
MS.gene42903	MS.gene63150	PPI
MS.gene81712	MS.gene63150	PPI
MS.gene026680	MS.gene63150	PPI
MS.gene63150	MS.gene024297	PPI
MS.gene63150	MS.gene059269	PPI
MS.gene048747	MS.gene63150	PPI
MS.gene63150	MS.gene057934	PPI
MS.gene63150	MS.gene37537	PPI
MS.gene63150	MS.gene024841	PPI
MS.gene22200	MS.gene63150	PPI
MS.gene63150	MS.gene069786	PPI
MS.gene63150	MS.gene42010	PPI
MS.gene63150	MS.gene025158	PPI
MS.gene26038	MS.gene63150	PPI
MS.gene63150	MS.gene27329	PPI
MS.gene63150	MS.gene41393	PPI
MS.gene073573	MS.gene63150	PPI
MS.gene031967	MS.gene63150	PPI
MS.gene63150	MS.gene016632	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63150.t1	MTR_0010s0370	71.585	183	52	0	1	183	1	183	2.86e-92	270
MS.gene63150.t1	MTR_0010s0350	71.585	183	52	0	1	183	1	183	2.86e-92	270
MS.gene63150.t1	MTR_1g036720	62.727	110	41	0	1	110	1	110	4.28e-46	149
MS.gene63150.t1	MTR_1g036710	51.754	114	21	1	132	245	1	80	1.58e-29	112
MS.gene63150.t1	MTR_6g033215	29.167	168	119	0	32	199	127	294	1.35e-27	107
MS.gene63150.t1	MTR_7g089990	45.370	108	57	1	104	209	86	193	3.09e-21	89.0
MS.gene63150.t1	MTR_3g053920	33.136	169	94	3	3	171	7	156	7.84e-20	86.3
MS.gene63150.t1	MTR_2g061500	37.190	121	74	1	7	127	11	129	1.25e-18	79.7
MS.gene63150.t1	MTR_4g103530	39.080	87	53	0	59	145	7	93	7.04e-16	73.9
MS.gene63150.t1	MTR_7g106180	27.397	146	97	4	34	175	26	166	1.99e-13	67.8
MS.gene63150.t1	MTR_7g011710	39.706	68	41	0	73	140	21	88	3.02e-12	63.2
MS.gene63150.t1	MTR_3g024240	33.058	121	79	1	82	200	55	175	1.06e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63150.t1	AT3G31430	23.944	213	158	1	3	215	103	311	2.42e-20	88.6
MS.gene63150.t1	AT2G41590	27.650	217	145	5	4	215	9	218	2.26e-19	86.3
MS.gene63150.t1	AT2G17920	25.234	214	154	2	4	215	9	218	1.75e-18	83.2
MS.gene63150.t1	AT5G36228	23.148	216	158	3	3	215	8	218	1.58e-17	81.3
MS.gene63150.t1	AT2G13450	23.478	230	169	2	4	228	9	236	4.02e-17	80.1
MS.gene63150.t1	AT5G25200	24.057	212	159	1	4	215	9	218	1.82e-16	78.2
MS.gene63150.t1	AT5G18636	24.057	212	159	1	4	215	9	218	1.91e-16	78.2
MS.gene63150.t1	AT2G02103	25.346	217	150	5	4	215	9	218	2.03e-16	78.2
MS.gene63150.t1	AT3G57587	27.517	149	105	2	68	215	72	218	1.57e-15	75.5
MS.gene63150.t1	AT3G47920	27.703	148	100	3	71	215	4	147	4.57e-14	70.9
MS.gene63150.t1	AT5G36225	32.222	90	61	0	121	210	17	106	2.15e-13	67.8
MS.gene63150.t1	AT4G02000	25.490	153	107	2	81	228	6	156	2.80e-13	68.6

Find 65 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTCCAGATGATGATTAAAT+TGG	0.209003	8.1:+43824307	None:intergenic
ATATGTCCCCTTCTGGATTC+AGG	0.247997	8.1:-43824184	MS.gene63150:CDS
ATGATGACGGTACTCGACTA+TGG	0.315152	8.1:-43823864	MS.gene63150:CDS
CCCTCCAATATGTCCCCTTC+TGG	0.328211	8.1:-43824191	MS.gene63150:CDS
CTGCTACTTCTGTGGTATGC+TGG	0.335306	8.1:-43823929	MS.gene63150:CDS
TATTGGAGGGTTACTTGTTT+CGG	0.349115	8.1:+43824204	None:intergenic
CACGTCTGCGCCTTGTTGGC+AGG	0.349635	8.1:-43824443	MS.gene63150:CDS
GATTCACGTCTGCGCCTTGT+TGG	0.364545	8.1:-43824447	MS.gene63150:CDS
GAAAGAAATTGCTAACTTCA+TGG	0.386091	8.1:-43824121	MS.gene63150:CDS
ATGGGCCTTTCTTCAAGATT+CGG	0.398217	8.1:+43824280	None:intergenic
CGATCGGCGTTTCGTCGAAC+TGG	0.400374	8.1:+43824478	None:intergenic
CAGGTTCACGATATACCATT+TGG	0.400597	8.1:-43824165	MS.gene63150:CDS
TGTTTATCCACTCGTATCTC+TGG	0.400750	8.1:+43823838	None:intergenic
GAATCCAGAAGGGGACATAT+TGG	0.417051	8.1:+43824187	None:intergenic
GTTCCAATTTAATCATCATC+TGG	0.428572	8.1:-43824310	MS.gene63150:CDS
GATAAACAATGCTATTCTAA+TGG	0.433795	8.1:-43823823	MS.gene63150:CDS
CATATATGATCGAAGGAAGT+TGG	0.434833	8.1:+43824065	None:intergenic
TGGAGAAGATGGAAACCTTT+CGG	0.437140	8.1:-43824383	MS.gene63150:CDS
CAAATTCAAGTATGAGCGCT+TGG	0.437365	8.1:-43823959	MS.gene63150:CDS
ATACTTGAATTTGATATAGC+AGG	0.454710	8.1:+43823969	None:intergenic
TGCTACTTCTGTGGTATGCT+GGG	0.460844	8.1:-43823928	MS.gene63150:CDS
TTCTCTGACATCAGACCAAA+TGG	0.470027	8.1:+43824150	None:intergenic
GCTTAATGACAGTTGGTGTA+TGG	0.470757	8.1:+43824524	None:intergenic
TCCTTACCAACCATCCCATT+CGG	0.478450	8.1:-43824419	MS.gene63150:CDS
GTCTGATGTCAGAGAAAGTA+GGG	0.501505	8.1:-43824143	MS.gene63150:CDS
CTACATGATGATGGAGAAGA+TGG	0.506371	8.1:-43824394	MS.gene63150:CDS
TGATGACGGTACTCGACTAT+GGG	0.509500	8.1:-43823863	MS.gene63150:CDS
ATCATCATGTAGGACCGAAT+GGG	0.513118	8.1:+43824405	None:intergenic
GTATTTCGACAACCACCTCT+TGG	0.513468	8.1:-43824256	MS.gene63150:CDS
CATCTTCTCCATCATCATGT+AGG	0.515273	8.1:+43824395	None:intergenic
TGAATATCGACTCCCTTCAC+AGG	0.516321	8.1:+43824357	None:intergenic
CATTCGGTCCTACATGATGA+TGG	0.524262	8.1:-43824403	MS.gene63150:CDS
AAATTCAAGTATGAGCGCTT+GGG	0.530788	8.1:-43823958	MS.gene63150:CDS
AAAGAAATTGCTAACTTCAT+GGG	0.532221	8.1:-43824120	MS.gene63150:CDS
GGTAACTACTGCTACTTCTG+TGG	0.533684	8.1:-43823937	MS.gene63150:CDS
ATGAACCGAATCTTGAAGAA+AGG	0.541399	8.1:-43824285	MS.gene63150:CDS
ACCGAATGGGATGGTTGGTA+AGG	0.546611	8.1:+43824418	None:intergenic
CTTCATCGCTTAATGACAGT+TGG	0.559041	8.1:+43824517	None:intergenic
GGTCTGATGTCAGAGAAAGT+AGG	0.569569	8.1:-43824144	MS.gene63150:CDS
CATCATCATGTAGGACCGAA+TGG	0.570763	8.1:+43824404	None:intergenic
TCCAGAAGGGGACATATTGG+AGG	0.571919	8.1:+43824190	None:intergenic
AACCTTTCGGAATCCTGTGA+AGG	0.572463	8.1:-43824370	MS.gene63150:CDS
TGGTAAGGAACCTGCCAACA+AGG	0.590890	8.1:+43824433	None:intergenic
ACCTTTCGGAATCCTGTGAA+GGG	0.593563	8.1:-43824369	MS.gene63150:CDS
CGTGAATCAGCAGTGACGAT+CGG	0.594229	8.1:+43824462	None:intergenic
ATCGTGAACCTGAATCCAGA+AGG	0.597545	8.1:+43824176	None:intergenic
GTAGGACCGAATGGGATGGT+TGG	0.599242	8.1:+43824413	None:intergenic
TCGTGAACCTGAATCCAGAA+GGG	0.603562	8.1:+43824177	None:intergenic
TCCCTTCACAGGATTCCGAA+AGG	0.606498	8.1:+43824368	None:intergenic
AGGTGGTTGTCGAAATACCA+TGG	0.619450	8.1:+43824261	None:intergenic
CTCGATGTCATGTCGGAGAG+CGG	0.623939	8.1:-43824231	MS.gene63150:CDS
AAACAATGCTATTCTAATGG+CGG	0.624036	8.1:-43823820	MS.gene63150:CDS
CTTGGTACTCGATGTCATGT+CGG	0.627819	8.1:-43824238	MS.gene63150:CDS
GGTGGTTGTCGAAATACCAT+GGG	0.630931	8.1:+43824262	None:intergenic
TCATGTAGGACCGAATGGGA+TGG	0.636917	8.1:+43824409	None:intergenic
GATATTCAAGAAATTGAGCA+AGG	0.637924	8.1:-43824342	MS.gene63150:CDS
CCAGAAGGGGACATATTGGA+GGG	0.657555	8.1:+43824191	None:intergenic
GAATCTTGAAGAAAGGCCCA+TGG	0.667358	8.1:-43824278	MS.gene63150:CDS
GAATGCGCATATATGATCGA+AGG	0.667457	8.1:+43824058	None:intergenic
CGTGAACCTGAATCCAGAAG+GGG	0.689521	8.1:+43824178	None:intergenic
TATGCTATAACAGATGATGA+CGG	0.691050	8.1:-43823877	MS.gene63150:CDS
GATGACGGTACTCGACTATG+GGG	0.695256	8.1:-43823862	MS.gene63150:CDS
TGACATCGAGTACCAAGAGG+TGG	0.705302	8.1:+43824244	None:intergenic
ATGGGGTCCAGAGATACGAG+TGG	0.708048	8.1:-43823845	MS.gene63150:CDS
ACATGACATCGAGTACCAAG+AGG	0.719032	8.1:+43824241	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAGATTAAAAAACCA+GGG	-	chr8.1:43824342-43824361	MS.gene63150:CDS	15.0%
!!	AAAAAAGATTAAAAAACCAG+GGG	-	chr8.1:43824343-43824362	MS.gene63150:CDS	20.0%
!!	CAAAAAAAGATTAAAAAACC+AGG	-	chr8.1:43824341-43824360	MS.gene63150:CDS	20.0%
!!!	CTGGTTTTTTAATCTTTTTT+TGG	+	chr8.1:43824343-43824362	None:intergenic	20.0%
!	AAAAAGATTAAAAAACCAGG+GGG	-	chr8.1:43824344-43824363	MS.gene63150:CDS	25.0%
!	AAAGAAATTGCTAACTTCAT+GGG	-	chr8.1:43824221-43824240	MS.gene63150:CDS	25.0%
!	ATACTTGAATTTGATATAGC+AGG	+	chr8.1:43824375-43824394	None:intergenic	25.0%
!	GATAAACAATGCTATTCTAA+TGG	-	chr8.1:43824518-43824537	MS.gene63150:CDS	25.0%
	AAACAATGCTATTCTAATGG+CGG	-	chr8.1:43824521-43824540	MS.gene63150:CDS	30.0%
	GAAAGAAATTGCTAACTTCA+TGG	-	chr8.1:43824220-43824239	MS.gene63150:CDS	30.0%
	GATATTCAAGAAATTGAGCA+AGG	-	chr8.1:43823999-43824018	MS.gene63150:CDS	30.0%
	GTTCCAATTTAATCATCATC+TGG	-	chr8.1:43824031-43824050	MS.gene63150:CDS	30.0%
	TATGCTATAACAGATGATGA+CGG	-	chr8.1:43824464-43824483	MS.gene63150:CDS	30.0%
	TGTCCAGATGATGATTAAAT+TGG	+	chr8.1:43824037-43824056	None:intergenic	30.0%
!!!	ATCTTTTTTTGGCGTTTTAG+AGG	+	chr8.1:43824332-43824351	None:intergenic	30.0%
	AAATTCAAGTATGAGCGCTT+GGG	-	chr8.1:43824383-43824402	MS.gene63150:CDS	35.0%
	ATGAACCGAATCTTGAAGAA+AGG	-	chr8.1:43824056-43824075	MS.gene63150:CDS	35.0%
	CATATATGATCGAAGGAAGT+TGG	+	chr8.1:43824279-43824298	None:intergenic	35.0%
	TATTGGAGGGTTACTTGTTT+CGG	+	chr8.1:43824140-43824159	None:intergenic	35.0%
	ATCATCATGTAGGACCGAAT+GGG	+	chr8.1:43823939-43823958	None:intergenic	40.0%
	ATGGGCCTTTCTTCAAGATT+CGG	+	chr8.1:43824064-43824083	None:intergenic	40.0%
	CAAATTCAAGTATGAGCGCT+TGG	-	chr8.1:43824382-43824401	MS.gene63150:CDS	40.0%
	CAGGTTCACGATATACCATT+TGG	-	chr8.1:43824176-43824195	MS.gene63150:CDS	40.0%
	CATCTTCTCCATCATCATGT+AGG	+	chr8.1:43823949-43823968	None:intergenic	40.0%
	CTTCATCGCTTAATGACAGT+TGG	+	chr8.1:43823827-43823846	None:intergenic	40.0%
	GAATGCGCATATATGATCGA+AGG	+	chr8.1:43824286-43824305	None:intergenic	40.0%
	GTCTGATGTCAGAGAAAGTA+GGG	-	chr8.1:43824198-43824217	MS.gene63150:CDS	40.0%
	TGGAGAAGATGGAAACCTTT+CGG	-	chr8.1:43823958-43823977	MS.gene63150:CDS	40.0%
	TGTTTATCCACTCGTATCTC+TGG	+	chr8.1:43824506-43824525	None:intergenic	40.0%
	TTCTCTGACATCAGACCAAA+TGG	+	chr8.1:43824194-43824213	None:intergenic	40.0%
!	GCTTAATGACAGTTGGTGTA+TGG	+	chr8.1:43823820-43823839	None:intergenic	40.0%
!!	CTACATGATGATGGAGAAGA+TGG	-	chr8.1:43823947-43823966	MS.gene63150:CDS	40.0%
	AACCTTTCGGAATCCTGTGA+AGG	-	chr8.1:43823971-43823990	MS.gene63150:CDS	45.0%
	ACATGACATCGAGTACCAAG+AGG	+	chr8.1:43824103-43824122	None:intergenic	45.0%
	ACCTTTCGGAATCCTGTGAA+GGG	-	chr8.1:43823972-43823991	MS.gene63150:CDS	45.0%
	AGGTGGTTGTCGAAATACCA+TGG	+	chr8.1:43824083-43824102	None:intergenic	45.0%
	ATATGTCCCCTTCTGGATTC+AGG	-	chr8.1:43824157-43824176	MS.gene63150:CDS	45.0%
	ATCGTGAACCTGAATCCAGA+AGG	+	chr8.1:43824168-43824187	None:intergenic	45.0%
	CATCATCATGTAGGACCGAA+TGG	+	chr8.1:43823940-43823959	None:intergenic	45.0%
	CATTCGGTCCTACATGATGA+TGG	-	chr8.1:43823938-43823957	MS.gene63150:CDS	45.0%
	CTTGGTACTCGATGTCATGT+CGG	-	chr8.1:43824103-43824122	MS.gene63150:CDS	45.0%
	GAATCCAGAAGGGGACATAT+TGG	+	chr8.1:43824157-43824176	None:intergenic	45.0%
	GAATCTTGAAGAAAGGCCCA+TGG	-	chr8.1:43824063-43824082	MS.gene63150:CDS	45.0%
	GGTCTGATGTCAGAGAAAGT+AGG	-	chr8.1:43824197-43824216	MS.gene63150:CDS	45.0%
	GGTGGTTGTCGAAATACCAT+GGG	+	chr8.1:43824082-43824101	None:intergenic	45.0%
	GTATTTCGACAACCACCTCT+TGG	-	chr8.1:43824085-43824104	MS.gene63150:CDS	45.0%
	TCCTTACCAACCATCCCATT+CGG	-	chr8.1:43823922-43823941	MS.gene63150:CDS	45.0%
	TCGTGAACCTGAATCCAGAA+GGG	+	chr8.1:43824167-43824186	None:intergenic	45.0%
!	GGTAACTACTGCTACTTCTG+TGG	-	chr8.1:43824404-43824423	MS.gene63150:CDS	45.0%
!	TGCTACTTCTGTGGTATGCT+GGG	-	chr8.1:43824413-43824432	MS.gene63150:CDS	45.0%
!!	ATGATGACGGTACTCGACTA+TGG	-	chr8.1:43824477-43824496	MS.gene63150:CDS	45.0%
!!	TGAATATCGACTCCCTTCAC+AGG	+	chr8.1:43823987-43824006	None:intergenic	45.0%
!!	TGATGACGGTACTCGACTAT+GGG	-	chr8.1:43824478-43824497	MS.gene63150:CDS	45.0%
	ATATAGCAGGAATCTCCCCC+TGG	+	chr8.1:43824362-43824381	None:intergenic	50.0%
	CCAGAAGGGGACATATTGGA+GGG	+	chr8.1:43824153-43824172	None:intergenic	50.0%
	CGTGAACCTGAATCCAGAAG+GGG	+	chr8.1:43824166-43824185	None:intergenic	50.0%
	CGTGAATCAGCAGTGACGAT+CGG	+	chr8.1:43823882-43823901	None:intergenic	50.0%
	TCATGTAGGACCGAATGGGA+TGG	+	chr8.1:43823935-43823954	None:intergenic	50.0%
	TCCAGAAGGGGACATATTGG+AGG	+	chr8.1:43824154-43824173	None:intergenic	50.0%
	TCCCTTCACAGGATTCCGAA+AGG	+	chr8.1:43823976-43823995	None:intergenic	50.0%
	TGACATCGAGTACCAAGAGG+TGG	+	chr8.1:43824100-43824119	None:intergenic	50.0%
	TGGTAAGGAACCTGCCAACA+AGG	+	chr8.1:43823911-43823930	None:intergenic	50.0%
!	ACCGAATGGGATGGTTGGTA+AGG	+	chr8.1:43823926-43823945	None:intergenic	50.0%
!	CTGCTACTTCTGTGGTATGC+TGG	-	chr8.1:43824412-43824431	MS.gene63150:CDS	50.0%
!	GATGACGGTACTCGACTATG+GGG	-	chr8.1:43824479-43824498	MS.gene63150:CDS	50.0%
	ATGGGGTCCAGAGATACGAG+TGG	-	chr8.1:43824496-43824515	MS.gene63150:CDS	55.0%
	CCCTCCAATATGTCCCCTTC+TGG	-	chr8.1:43824150-43824169	MS.gene63150:CDS	55.0%
	CTCGATGTCATGTCGGAGAG+CGG	-	chr8.1:43824110-43824129	MS.gene63150:CDS	55.0%
	GATTCACGTCTGCGCCTTGT+TGG	-	chr8.1:43823894-43823913	MS.gene63150:CDS	55.0%
	GTAGGACCGAATGGGATGGT+TGG	+	chr8.1:43823931-43823950	None:intergenic	55.0%
	CGATCGGCGTTTCGTCGAAC+TGG	+	chr8.1:43823866-43823885	None:intergenic	60.0%
!	CACGTCTGCGCCTTGTTGGC+AGG	-	chr8.1:43823898-43823917	MS.gene63150:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	43823810	43824553	43823810	ID=MS.gene63150
chr8.1	mRNA	43823810	43824553	43823810	ID=MS.gene63150.t1;Parent=MS.gene63150
chr8.1	exon	43823810	43824553	43823810	ID=MS.gene63150.t1.exon1;Parent=MS.gene63150.t1
chr8.1	CDS	43823810	43824553	43823810	ID=cds.MS.gene63150.t1;Parent=MS.gene63150.t1

Gene Sequence

>MS.gene63150

ATGGCTTCCATACACCAACTGTCATTAAGCGATGAAGATGACGAACTCGAATTCCAGTTCGACGAAACGCCGATCGTCACTGCTGATTCACGTCTGCGCCTTGTTGGCAGGTTCCTTACCAACCATCCCATTCGGTCCTACATGATGATGGAGAAGATGGAAACCTTTCGGAATCCTGTGAAGGGAGTCGATATTCAAGAAATTGAGCAAGGTACGTATACGTTCCAATTTAATCATCATCTGGACATGAACCGAATCTTGAAGAAAGGCCCATGGTATTTCGACAACCACCTCTTGGTACTCGATGTCATGTCGGAGAGCGGCAATCCGAAACAAGTAACCCTCCAATATGTCCCCTTCTGGATTCAGGTTCACGATATACCATTTGGTCTGATGTCAGAGAAAGTAGGGAAAGAAATTGCTAACTTCATGGGTGAATTCTTAGAATACGATGCTAAAAACAACTCCAACTTCCTTCGATCATATATGCGCATTCGAGTACTGCTTGACGTCACTAAACCTCTAAAACGCCAAAAAAAGATTAAAAAACCAGGGGGAGATTCCTGCTATATCAAATTCAAGTATGAGCGCTTGGGTAACTACTGCTACTTCTGTGGTATGCTGGGACATATAGAAGATTATTGTGAAAAGCTATATGCTATAACAGATGATGACGGTACTCGACTATGGGGTCCAGAGATACGAGTGGATAAACAATGCTATTCTAATGGCGGCTCTAGATGA

Protein sequence

>MS.gene63150.t1

MASIHQLSLSDEDDELEFQFDETPIVTADSRLRLVGRFLTNHPIRSYMMMEKMETFRNPVKGVDIQEIEQGTYTFQFNHHLDMNRILKKGPWYFDNHLLVLDVMSESGNPKQVTLQYVPFWIQVHDIPFGLMSEKVGKEIANFMGEFLEYDAKNNSNFLRSYMRIRVLLDVTKPLKRQKKIKKPGGDSCYIKFKYERLGNYCYFCGMLGHIEDYCEKLYAITDDDGTRLWGPEIRVDKQCYSNGGSR