AlfalfaGEDB | MS.gene013193

M. sativa cultivar XinJiangDaYe / MS.gene013193

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013193.t1	AES70414.1	53.8	346	93	4	1	345	1	280	3.00E-89	339

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013193.t1	O04244	27.7	137	93	3	83	216	6	139	3.4e-09	64.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013193.t1	G7J017	53.8	346	93	4	1	345	1	280	2.1e-89	339.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene013193	MS.gene72520	PPI
MS.gene013193	MS.gene61452	PPI
MS.gene013193	MS.gene05231	PPI
MS.gene013193	MS.gene03149	PPI
MS.gene013193	MS.gene042559	PPI
MS.gene013193	MS.gene46948	PPI
MS.gene013193	MS.gene29321	PPI
MS.gene013193	MS.gene037049	PPI
MS.gene013193	MS.gene24278	PPI
MS.gene013193	MS.gene34618	PPI
MS.gene013193	MS.gene59460	PPI
MS.gene013193	MS.gene060773	PPI
MS.gene013193	MS.gene66650	PPI
MS.gene013193	MS.gene009121	PPI
MS.gene013193	MS.gene08713	PPI
MS.gene013193	MS.gene09589	PPI
MS.gene013193	MS.gene70932	PPI
MS.gene038676	MS.gene013193	PPI
MS.gene013193	MS.gene024405	PPI
MS.gene013193	MS.gene067340	PPI
MS.gene013193	MS.gene021965	PPI
MS.gene013193	MS.gene018196	PPI
MS.gene013193	MS.gene32415	PPI
MS.gene013193	MS.gene24070	PPI
MS.gene013193	MS.gene83772	PPI
MS.gene013193	MS.gene059736	PPI
MS.gene013193	MS.gene85538	PPI
MS.gene013193	MS.gene26844	PPI
MS.gene013193	MS.gene64178	PPI
MS.gene013193	MS.gene96174	PPI
MS.gene013193	MS.gene074257	PPI
MS.gene013193	MS.gene04749	PPI
MS.gene013193	MS.gene073573	PPI
MS.gene013193	MS.gene69490	PPI
MS.gene013193	MS.gene44144	PPI
MS.gene013193	MS.gene05576	PPI
MS.gene013193	MS.gene060718	PPI
MS.gene013193	MS.gene96109	PPI
MS.gene013193	MS.gene63743	PPI
MS.gene013193	MS.gene016102	PPI
MS.gene013193	MS.gene48477	PPI
MS.gene013193	MS.gene055223	PPI
MS.gene013193	MS.gene068629	PPI
MS.gene013193	MS.gene08106	PPI
MS.gene013193	MS.gene027169	PPI
MS.gene013193	MS.gene004417	PPI
MS.gene013193	MS.gene019177	PPI
MS.gene013193	MS.gene031967	PPI
MS.gene013193	MS.gene05436	PPI
MS.gene013193	MS.gene038623	PPI
MS.gene013193	MS.gene055560	PPI
MS.gene013193	MS.gene009213	PPI
MS.gene013193	MS.gene98127	PPI
MS.gene013193	MS.gene003264	PPI
MS.gene013193	MS.gene054652	PPI
MS.gene013193	MS.gene000634	PPI
MS.gene013193	MS.gene20246	PPI
MS.gene042559	MS.gene013193	PPI
MS.gene22200	MS.gene013193	PPI
MS.gene013193	MS.gene86849	PPI
MS.gene013193	MS.gene049512	PPI
MS.gene013193	MS.gene27329	PPI
MS.gene013193	MS.gene41393	PPI
MS.gene013193	MS.gene42903	PPI
MS.gene013193	MS.gene30208	PPI
MS.gene013193	MS.gene80362	PPI
MS.gene013193	MS.gene89815	PPI
MS.gene013193	MS.gene010656	PPI
MS.gene013193	MS.gene016632	PPI
MS.gene013193	MS.gene018474	PPI
MS.gene013193	MS.gene048088	PPI
MS.gene013193	MS.gene86868	PPI
MS.gene34812	MS.gene013193	PPI
MS.gene063214	MS.gene013193	PPI
MS.gene013193	MS.gene058889	PPI
MS.gene013193	MS.gene96163	PPI
MS.gene067340	MS.gene013193	PPI
MS.gene013193	MS.gene050679	PPI
MS.gene013193	MS.gene34463	PPI
MS.gene69495	MS.gene013193	PPI
MS.gene05357	MS.gene013193	PPI
MS.gene013193	MS.gene042614	PPI
MS.gene013193	MS.gene026680	PPI
MS.gene013193	MS.gene61499	PPI
MS.gene013193	MS.gene51804	PPI
MS.gene013193	MS.gene051449	PPI
MS.gene013193	MS.gene042651	PPI
MS.gene013193	MS.gene019165	PPI
MS.gene013193	MS.gene81194	PPI
MS.gene013193	MS.gene038394	PPI
MS.gene021968	MS.gene013193	PPI
MS.gene013193	MS.gene019001	PPI
MS.gene013193	MS.gene038676	PPI
MS.gene013193	MS.gene53904	PPI
MS.gene013193	MS.gene73261	PPI
MS.gene013193	MS.gene025675	PPI
MS.gene013193	MS.gene94654	PPI
MS.gene013193	MS.gene055546	PPI
MS.gene013193	MS.gene71637	PPI
MS.gene013193	MS.gene55209	PPI
MS.gene013193	MS.gene28718	PPI
MS.gene013193	MS.gene022929	PPI
MS.gene013193	MS.gene85295	PPI
MS.gene013193	MS.gene009781	PPI
MS.gene013193	MS.gene021647	PPI
MS.gene013193	MS.gene047954	PPI
MS.gene013193	MS.gene071784	PPI
MS.gene013193	MS.gene048342	PPI
MS.gene013193	MS.gene069624	PPI
MS.gene42903	MS.gene013193	PPI
MS.gene013193	MS.gene83979	PPI
MS.gene013193	MS.gene21066	PPI
MS.gene013193	MS.gene013890	PPI
MS.gene013193	MS.gene073582	PPI
MS.gene013193	MS.gene016719	PPI
MS.gene013193	MS.gene048072	PPI
MS.gene013193	MS.gene028714	PPI
MS.gene013193	MS.gene042027	PPI
MS.gene013193	MS.gene048706	PPI
MS.gene013193	MS.gene69586	PPI
MS.gene013193	MS.gene45083	PPI
MS.gene013193	MS.gene049561	PPI
MS.gene013193	MS.gene008658	PPI
MS.gene013193	MS.gene47792	PPI
MS.gene013193	MS.gene49057	PPI
MS.gene94654	MS.gene013193	PPI
MS.gene013193	MS.gene03936	PPI
MS.gene013193	MS.gene43393	PPI
MS.gene013193	MS.gene08708	PPI
MS.gene013193	MS.gene86275	PPI
MS.gene013193	MS.gene034643	PPI
MS.gene035626	MS.gene013193	PPI
MS.gene013193	MS.gene60059	PPI
MS.gene013193	MS.gene038098	PPI
MS.gene013193	MS.gene071320	PPI
MS.gene013193	MS.gene012019	PPI
MS.gene013193	MS.gene46941	PPI
MS.gene30208	MS.gene013193	PPI
MS.gene013193	MS.gene012889	PPI
MS.gene013193	MS.gene038757	PPI
MS.gene013193	MS.gene000506	PPI
MS.gene013193	MS.gene015248	PPI
MS.gene013193	MS.gene030090	PPI
MS.gene013193	MS.gene39956	PPI
MS.gene95561	MS.gene013193	PPI
MS.gene013193	MS.gene028716	PPI
MS.gene013193	MS.gene74639	PPI
MS.gene013193	MS.gene071133	PPI
MS.gene013193	MS.gene60266	PPI
MS.gene013193	MS.gene98467	PPI
MS.gene013193	MS.gene013644	PPI
MS.gene013193	MS.gene024522	PPI
MS.gene065627	MS.gene013193	PPI
MS.gene013193	MS.gene54051	PPI
MS.gene013193	MS.gene22111	PPI
MS.gene57019	MS.gene013193	PPI
MS.gene013193	MS.gene049610	PPI
MS.gene013193	MS.gene010290	PPI
MS.gene013193	MS.gene93396	PPI
MS.gene013193	MS.gene37955	PPI
MS.gene013193	MS.gene95561	PPI
MS.gene013193	MS.gene05358	PPI
MS.gene013193	MS.gene92517	PPI
MS.gene013193	MS.gene34197	PPI
MS.gene013193	MS.gene045875	PPI
MS.gene013193	MS.gene010890	PPI
MS.gene013193	MS.gene84790	PPI
MS.gene013193	MS.gene055683	PPI
MS.gene013193	MS.gene061579	PPI
MS.gene013193	MS.gene05319	PPI
MS.gene013193	MS.gene69495	PPI
MS.gene013193	MS.gene032311	PPI
MS.gene013193	MS.gene41979	PPI
MS.gene013193	MS.gene04970	PPI
MS.gene008275	MS.gene013193	PPI
MS.gene013193	MS.gene26038	PPI
MS.gene013193	MS.gene060788	PPI
MS.gene013193	MS.gene98616	PPI
MS.gene013193	MS.gene43924	PPI
MS.gene013193	MS.gene23521	PPI
MS.gene013193	MS.gene41643	PPI
MS.gene013193	MS.gene77990	PPI
MS.gene013193	MS.gene062511	PPI
MS.gene013193	MS.gene008861	PPI
MS.gene013193	MS.gene065627	PPI
MS.gene013193	MS.gene027994	PPI
MS.gene013193	MS.gene96541	PPI
MS.gene013193	MS.gene26433	PPI
MS.gene013193	MS.gene007022	PPI
MS.gene013193	MS.gene045614	PPI
MS.gene013193	MS.gene064966	PPI
MS.gene013193	MS.gene69584	PPI
MS.gene013193	MS.gene036423	PPI
MS.gene013193	MS.gene52349	PPI
MS.gene013193	MS.gene76420	PPI
MS.gene013193	MS.gene30136	PPI
MS.gene013193	MS.gene46353	PPI
MS.gene013193	MS.gene65471	PPI
MS.gene013193	MS.gene060386	PPI
MS.gene013193	MS.gene61651	PPI
MS.gene013193	MS.gene29406	PPI
MS.gene72520	MS.gene013193	PPI
MS.gene013193	MS.gene63762	PPI
MS.gene96109	MS.gene013193	PPI
MS.gene013193	MS.gene09023	PPI
MS.gene013193	MS.gene88393	PPI
MS.gene013193	MS.gene054508	PPI
MS.gene013193	MS.gene95960	PPI
MS.gene013193	MS.gene053373	PPI
MS.gene013193	MS.gene041264	PPI
MS.gene013193	MS.gene35175	PPI
MS.gene013193	MS.gene63150	PPI
MS.gene013193	MS.gene035626	PPI
MS.gene013193	MS.gene44083	PPI
MS.gene013193	MS.gene000629	PPI
MS.gene013193	MS.gene072852	PPI
MS.gene013193	MS.gene65474	PPI
MS.gene013193	MS.gene018745	PPI
MS.gene013193	MS.gene025401	PPI
MS.gene013193	MS.gene34812	PPI
MS.gene013193	MS.gene57044	PPI
MS.gene013193	MS.gene015093	PPI
MS.gene013193	MS.gene04134	PPI
MS.gene013193	MS.gene06164	PPI
MS.gene013193	MS.gene50392	PPI
MS.gene013193	MS.gene22730	PPI
MS.gene013193	MS.gene000379	PPI
MS.gene016102	MS.gene013193	PPI
MS.gene46941	MS.gene013193	PPI
MS.gene96541	MS.gene013193	PPI
MS.gene013193	MS.gene032299	PPI
MS.gene013193	MS.gene017586	PPI
MS.gene013193	MS.gene037634	PPI
MS.gene013193	MS.gene057828	PPI
MS.gene013193	MS.gene08606	PPI
MS.gene013193	MS.gene048747	PPI
MS.gene013193	MS.gene000640	PPI
MS.gene025873	MS.gene013193	PPI
MS.gene013193	MS.gene061850	PPI
MS.gene013193	MS.gene09517	PPI
MS.gene013193	MS.gene98756	PPI
MS.gene013193	MS.gene87845	PPI
MS.gene013193	MS.gene57019	PPI
MS.gene05358	MS.gene013193	PPI
MS.gene013193	MS.gene85308	PPI
MS.gene93396	MS.gene013193	PPI
MS.gene013193	MS.gene64130	PPI
MS.gene013193	MS.gene62249	PPI
MS.gene013193	MS.gene58419	PPI
MS.gene013193	MS.gene046089	PPI
MS.gene013193	MS.gene053747	PPI
MS.gene013193	MS.gene28545	PPI
MS.gene013193	MS.gene021555	PPI
MS.gene013193	MS.gene36069	PPI
MS.gene013193	MS.gene71675	PPI
MS.gene013193	MS.gene044729	PPI
MS.gene56991	MS.gene013193	PPI
MS.gene013193	MS.gene008275	PPI
MS.gene013193	MS.gene60390	PPI
MS.gene013193	MS.gene039692	PPI
MS.gene20246	MS.gene013193	PPI
MS.gene013193	MS.gene55682	PPI
MS.gene28545	MS.gene013193	PPI
MS.gene013193	MS.gene002590	PPI
MS.gene013193	MS.gene81712	PPI
MS.gene013193	MS.gene008927	PPI
MS.gene013193	MS.gene005120	PPI
MS.gene013193	MS.gene65546	PPI
MS.gene013193	MS.gene025873	PPI
MS.gene013193	MS.gene05357	PPI
MS.gene013193	MS.gene024297	PPI
MS.gene013193	MS.gene059269	PPI
MS.gene013193	MS.gene35202	PPI
MS.gene013193	MS.gene22200	PPI
MS.gene013193	MS.gene057934	PPI
MS.gene013193	MS.gene37537	PPI
MS.gene013193	MS.gene024841	PPI
MS.gene013193	MS.gene069786	PPI
MS.gene013193	MS.gene42010	PPI
MS.gene013193	MS.gene025158	PPI
MS.gene013193	MS.gene40828	PPI
MS.gene013193	MS.gene87529	PPI
MS.gene013193	MS.gene51799	PPI
MS.gene013193	MS.gene98471	PPI
MS.gene013193	MS.gene062067	PPI
MS.gene013193	MS.gene97410	PPI
MS.gene013193	MS.gene56991	PPI
MS.gene69490	MS.gene013193	PPI
MS.gene015248	MS.gene013193	PPI
MS.gene013193	MS.gene013676	PPI
MS.gene013193	MS.gene40469	PPI
MS.gene08106	MS.gene013193	PPI
MS.gene013193	MS.gene65623	PPI
MS.gene013193	MS.gene021968	PPI
MS.gene013193	MS.gene012748	PPI
MS.gene013193	MS.gene063214	PPI
MS.gene013193	MS.gene66651	PPI
MS.gene013193	MS.gene025987	PPI
MS.gene013193	MS.gene043140	PPI
MS.gene013193	MS.gene02896	PPI
MS.gene013193	MS.gene71588	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013193.t1	MTR_3g053920	52.722	349	92	5	1	345	1	280	1.76e-112	330
MS.gene013193.t1	MTR_2g061500	75.969	129	31	0	1	129	1	129	6.68e-69	213
MS.gene013193.t1	MTR_4g103530	73.196	97	26	0	55	151	1	97	1.05e-45	155
MS.gene013193.t1	MTR_7g011710	74.118	85	22	0	55	139	1	85	2.21e-39	138
MS.gene013193.t1	MTR_6g033215	35.838	173	109	1	29	199	122	294	2.76e-36	134
MS.gene013193.t1	MTR_0010s0370	36.571	175	105	5	8	177	4	177	8.62e-30	114
MS.gene013193.t1	MTR_0010s0350	36.571	175	105	5	8	177	4	177	8.62e-30	114
MS.gene013193.t1	MTR_8g463110	56.122	98	35	2	289	385	1	91	1.08e-15	79.3
MS.gene013193.t1	MTR_1g036720	31.731	104	69	1	11	112	7	110	5.39e-13	65.5
MS.gene013193.t1	MTR_4g020545	34.483	116	60	3	63	178	79	178	9.18e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013193.t1	AT2G17920	25.701	214	153	3	7	216	8	219	3.29e-17	82.0
MS.gene013193.t1	AT5G36228	26.904	197	137	3	36	227	39	233	1.78e-16	80.5
MS.gene013193.t1	AT3G31430	28.108	185	121	5	36	215	134	311	2.80e-15	76.6
MS.gene013193.t1	AT2G41590	28.472	144	95	3	77	216	80	219	1.02e-14	75.5
MS.gene013193.t1	AT2G13450	26.776	183	128	3	36	215	39	218	1.84e-14	74.7
MS.gene013193.t1	AT3G57587	26.056	142	101	2	77	216	80	219	3.06e-14	73.9
MS.gene013193.t1	AT3G47920	25.352	142	102	2	77	216	9	148	5.18e-12	66.6

Find 103 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTAATTCATTTGGATATC+TGG	0.113881	3.2:+81898460	MS.gene013193:CDS
CTGCTTGATCGGTCGTGTTT+TGG	0.188329	3.2:+81898215	MS.gene013193:CDS
CGGACGATGGAGTACGTAAT+TGG	0.209232	3.2:+81898781	MS.gene013193:CDS
CACTGTTCCTCTAATTCATT+TGG	0.259561	3.2:+81898452	MS.gene013193:CDS
GATATCTGGGTGCAGGTCTA+TGG	0.275435	3.2:+81898474	MS.gene013193:CDS
CAGATTCAGCAGATAGGTTA+AGG	0.317540	3.2:-81898170	None:intergenic
TTTACTGCAGGGTCGTTCTA+TGG	0.328442	3.2:+81899124	MS.gene013193:CDS
AAACCTGTTACGACTCGTAT+AGG	0.344071	3.2:+81898816	MS.gene013193:CDS
CCTATATGCGCCTCAAAGTC+TGG	0.351054	3.2:+81898595	MS.gene013193:CDS
CAGGGTCGTTCTATGGTACT+TGG	0.351644	3.2:+81899131	MS.gene013193:CDS
CAAGGCATTGGCAGCTTTCT+CGG	0.355349	3.2:+81898531	MS.gene013193:CDS
TGTCACTGTACCGCTGAAAA+AGG	0.360408	3.2:+81898623	MS.gene013193:CDS
GGACGATGGAGTACGTAATT+GGG	0.361200	3.2:+81898782	MS.gene013193:CDS
CTTTGATGCGGCACAACATC+AGG	0.362158	3.2:-81898876	None:intergenic
CAGGTCTATGGATTGCCCTT+TGG	0.363802	3.2:+81898486	MS.gene013193:CDS
GACCCTATACGAGTCGTAAC+AGG	0.367774	3.2:-81898819	None:intergenic
CAGATATCCAAATGAATTAG+AGG	0.368589	3.2:-81898459	None:intergenic
TGCTACAAACAGATGGCTTC+AGG	0.376139	3.2:+81898845	MS.gene013193:CDS
TCTAATTCATTTGGATATCT+GGG	0.377178	3.2:+81898461	MS.gene013193:CDS
AATGGTGTTGCGACCATCAT+AGG	0.380913	3.2:-81898565	None:intergenic
TGCTATTAATGCTATTAAAC+AGG	0.387604	3.2:+81898995	MS.gene013193:CDS
TGTTACAGGTGTGGCATTCT+CGG	0.402641	3.2:+81898717	MS.gene013193:CDS
CATCAAGCTTGTGGTGGAAC+TGG	0.403105	3.2:-81898344	None:intergenic
AACCTGTTACGACTCGTATA+GGG	0.403226	3.2:+81898817	MS.gene013193:CDS
GTGTCCCGACTTGTTTGAAT+TGG	0.427535	3.2:+81898755	MS.gene013193:CDS
CTCGGCCTCAAATTTACTGC+AGG	0.434485	3.2:+81899112	MS.gene013193:CDS
GGTGCGGCCAGCAGAAGAAC+TGG	0.435350	3.2:-81898910	None:intergenic
AGGTTTCAGATTCAGCAGAT+AGG	0.439470	3.2:-81898176	None:intergenic
AGATTCAGCAGATAGGTTAA+GGG	0.441257	3.2:-81898169	None:intergenic
GAGGCCGAGACTCCTGTAGA+TGG	0.454282	3.2:-81899098	None:intergenic
ATGTCCCGTGCGTGGAAACC+TGG	0.455231	3.2:+81898276	MS.gene013193:CDS
GACCGATCAAGCAGTGATCT+AGG	0.458613	3.2:-81898206	None:intergenic
CTTTGGTTTCATTCAATCAA+AGG	0.459068	3.2:+81898503	MS.gene013193:CDS
GGAGGACTTTCCATTCTAAA+GGG	0.459478	3.2:-81899264	None:intergenic
CCAGACTTTGAGGCGCATAT+AGG	0.464440	3.2:-81898595	None:intergenic
GTGTGGCATTCTCGGACATT+CGG	0.466234	3.2:+81898725	MS.gene013193:CDS
ATAATAGTTACGCGTTTGCC+AGG	0.478326	3.2:-81898294	None:intergenic
GCAAAAGTGAAAGGAGCAGC+AGG	0.480319	3.2:-81899158	None:intergenic
GTATCCTTGATGAGGGTCCT+TGG	0.481984	3.2:+81898373	MS.gene013193:CDS
CGACTTGTTTGAATTGGACT+CGG	0.483849	3.2:+81898761	MS.gene013193:CDS
TCATTTGGATATCTGGGTGC+AGG	0.490055	3.2:+81898467	MS.gene013193:CDS
GTTTGCCAGGTTTCCACGCA+CGG	0.490364	3.2:-81898281	None:intergenic
TTCTCCATCTACAGGAGTCT+CGG	0.493364	3.2:+81899094	MS.gene013193:CDS
ATGCTTCCTTCTCCATCTAC+AGG	0.496559	3.2:+81899086	MS.gene013193:CDS
TTCCAAGCCAGTTCTTCTGC+TGG	0.498549	3.2:+81898903	MS.gene013193:CDS
GGTTTCATTCAATCAAAGGT+TGG	0.507457	3.2:+81898507	MS.gene013193:CDS
TGTTGCGACCATCATAGGTT+AGG	0.510688	3.2:-81898560	None:intergenic
GTCATGGAACGCATTGCTCC+TGG	0.512312	3.2:+81898417	MS.gene013193:CDS
TCAAAGGTTGGTCAAGGCAT+TGG	0.512470	3.2:+81898519	MS.gene013193:CDS
TTTGATTGAATGAAACCAAA+GGG	0.513269	3.2:-81898501	None:intergenic
TTTGGACGATCGTGAAATCA+AGG	0.514839	3.2:+81898233	MS.gene013193:CDS
TTTGAATTGGACTCGGACGA+TGG	0.520707	3.2:+81898768	MS.gene013193:CDS
ACGACCCTGCAGTAAATTTG+AGG	0.521272	3.2:-81899117	None:intergenic
TGACATCAATCCAGACTTTG+AGG	0.530919	3.2:-81898605	None:intergenic
CAACATTGACGGTCTTACCT+TGG	0.534894	3.2:+81898131	MS.gene013193:CDS
ATTCAATCAAAGGTTGGTCA+AGG	0.545909	3.2:+81898513	MS.gene013193:CDS
GAGTCCAATTCAAACAAGTC+GGG	0.547426	3.2:-81898759	None:intergenic
GTTGAGTTGTGTGGGATCCA+TGG	0.548620	3.2:-81898112	None:intergenic
TGAATTAGAGGAACAGTGAT+CGG	0.549035	3.2:-81898447	None:intergenic
CCCACACAACTCAACATTGA+CGG	0.549913	3.2:+81898120	MS.gene013193:CDS
TCAAAACAGAATTGTCAGCA+TGG	0.555985	3.2:-81898932	None:intergenic
ATGCGGCACAACATCAGGGA+TGG	0.556865	3.2:-81898871	None:intergenic
ATCCTAGATCACTGCTTGAT+CGG	0.559464	3.2:+81898204	MS.gene013193:CDS
TTTGTACCAGCAAAAGTGAA+AGG	0.560500	3.2:-81899167	None:intergenic
CAGAATTGTCAGCATGGGTG+CGG	0.561489	3.2:-81898926	None:intergenic
GGTACTCTCCTAACCTATGA+TGG	0.562210	3.2:+81898552	MS.gene013193:CDS
GGCCAGCAGAAGAACTGGCT+TGG	0.565580	3.2:-81898905	None:intergenic
TGCCGCTCGTATCCTTGATG+AGG	0.569722	3.2:+81898365	MS.gene013193:CDS
CAAAACAGAATTGTCAGCAT+GGG	0.573225	3.2:-81898931	None:intergenic
ACAGTGATCGGAACCACACC+AGG	0.573710	3.2:-81898435	None:intergenic
TTTGCCAGGTTTCCACGCAC+GGG	0.575795	3.2:-81898280	None:intergenic
GGTCTTACCTTGGCTGACGA+AGG	0.583933	3.2:+81898141	MS.gene013193:CDS
GCCGCTCGTATCCTTGATGA+GGG	0.585959	3.2:+81898366	MS.gene013193:CDS
GGACATTCGTTCACTGAAGT+AGG	0.587017	3.2:-81898259	None:intergenic
GGTCAAACCTTCGTCAGCCA+AGG	0.587526	3.2:-81898148	None:intergenic
TACGAGCGGCATCAAGCTTG+TGG	0.589023	3.2:-81898353	None:intergenic
CAGTGTCTACAGCAAGCATA+CGG	0.590441	3.2:-81898974	None:intergenic
TGCGGCACAACATCAGGGAT+GGG	0.590978	3.2:-81898870	None:intergenic
CATTCTCGGACATTCGGACA+AGG	0.591084	3.2:+81898731	MS.gene013193:CDS
TGATAACTTTCATATAGTCA+TGG	0.592825	3.2:+81898401	MS.gene013193:CDS
GTGAACGAATGTCCCGTGCG+TGG	0.593922	3.2:+81898268	MS.gene013193:CDS
GCTTGGAAGTTACTTTGATG+CGG	0.594151	3.2:-81898888	None:intergenic
GAGACTCCTGTAGATGGAGA+AGG	0.602523	3.2:-81899092	None:intergenic
ACCGTCAATGTTGAGTTGTG+TGG	0.602644	3.2:-81898121	None:intergenic
TGGAAAGTTCGTGCTAGCAA+TGG	0.606670	3.2:+81898648	MS.gene013193:CDS
CTTTGATTGAATGAAACCAA+AGG	0.608719	3.2:-81898502	None:intergenic
CGAGTCCAATTCAAACAAGT+CGG	0.610252	3.2:-81898760	None:intergenic
GAGCGGCATCAAGCTTGTGG+TGG	0.615991	3.2:-81898350	None:intergenic
GACGATGGAGTACGTAATTG+GGG	0.621097	3.2:+81898783	MS.gene013193:CDS
GGTCGGCTGCTACAAACAGA+TGG	0.622085	3.2:+81898838	MS.gene013193:CDS
CCGTCAATGTTGAGTTGTGT+GGG	0.623706	3.2:-81898120	None:intergenic
ATAACCAAGGACCCTCATCA+AGG	0.624768	3.2:-81898377	None:intergenic
GCGCATATAGGAGCTATGAA+TGG	0.625349	3.2:-81898583	None:intergenic
TTTGATGCGGCACAACATCA+GGG	0.625918	3.2:-81898875	None:intergenic
GTAGATGGAGAAGGAAGCAT+CGG	0.627617	3.2:-81899083	None:intergenic
ATCAAGCAGTGATCTAGGAT+AGG	0.632523	3.2:-81898201	None:intergenic
TCGGCCTCAAATTTACTGCA+GGG	0.638651	3.2:+81899113	MS.gene013193:CDS
GGAACGCATTGCTCCTGGTG+TGG	0.640570	3.2:+81898422	MS.gene013193:CDS
GCAGAGCAGTCTGAGCAGCG+AGG	0.684222	3.2:-81899022	None:intergenic
ACCCTCATCAAGGATACGAG+CGG	0.685761	3.2:-81898367	None:intergenic
TGAAAGTTATCATATAACCA+AGG	0.688479	3.2:-81898390	None:intergenic
GCAGTGATCTAGGATAGGAG+AGG	0.689001	3.2:-81898196	None:intergenic
TGTTACGACTCGTATAGGGT+CGG	0.713178	3.2:+81898821	MS.gene013193:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAAAAAAATAGTAACAA+AGG	-	chr3.2:81898676-81898695	None:intergenic	15.0%
!!!	TTTTTCAAATATGAGAAACT+GGG	+	chr3.2:81898687-81898706	MS.gene013193:CDS	20.0%
!!!	TTTTTTCAAATATGAGAAAC+TGG	+	chr3.2:81898686-81898705	MS.gene013193:CDS	20.0%
!	TCTAATTCATTTGGATATCT+GGG	+	chr3.2:81898461-81898480	MS.gene013193:CDS	25.0%
!	TGAAAGTTATCATATAACCA+AGG	-	chr3.2:81898393-81898412	None:intergenic	25.0%
!	TGATAACTTTCATATAGTCA+TGG	+	chr3.2:81898401-81898420	MS.gene013193:CDS	25.0%
!	TGCTATTAATGCTATTAAAC+AGG	+	chr3.2:81898995-81899014	MS.gene013193:CDS	25.0%
!	TTTGATTGAATGAAACCAAA+GGG	-	chr3.2:81898504-81898523	None:intergenic	25.0%
	CAAGCTAAAAATGTCACAAA+TGG	+	chr3.2:81899044-81899063	MS.gene013193:CDS	30.0%
	CAGATATCCAAATGAATTAG+AGG	-	chr3.2:81898462-81898481	None:intergenic	30.0%
	CTCTAATTCATTTGGATATC+TGG	+	chr3.2:81898460-81898479	MS.gene013193:CDS	30.0%
	CTTTGATTGAATGAAACCAA+AGG	-	chr3.2:81898505-81898524	None:intergenic	30.0%
!	CTTTGGTTTCATTCAATCAA+AGG	+	chr3.2:81898503-81898522	MS.gene013193:CDS	30.0%
	AAAAATGTCACAAATGGTGC+TGG	+	chr3.2:81899050-81899069	MS.gene013193:CDS	35.0%
	AGATTCAGCAGATAGGTTAA+GGG	-	chr3.2:81898172-81898191	None:intergenic	35.0%
	CAAAACAGAATTGTCAGCAT+GGG	-	chr3.2:81898934-81898953	None:intergenic	35.0%
	CACTGTTCCTCTAATTCATT+TGG	+	chr3.2:81898452-81898471	MS.gene013193:CDS	35.0%
	GGTTTCATTCAATCAAAGGT+TGG	+	chr3.2:81898507-81898526	MS.gene013193:CDS	35.0%
	TCAAAACAGAATTGTCAGCA+TGG	-	chr3.2:81898935-81898954	None:intergenic	35.0%
	TGAATTAGAGGAACAGTGAT+CGG	-	chr3.2:81898450-81898469	None:intergenic	35.0%
	TTTGTACCAGCAAAAGTGAA+AGG	-	chr3.2:81899170-81899189	None:intergenic	35.0%
!	ATTCAATCAAAGGTTGGTCA+AGG	+	chr3.2:81898513-81898532	MS.gene013193:CDS	35.0%
!!!	AACTGGGTGTTTTTTGTTAC+AGG	+	chr3.2:81898703-81898722	MS.gene013193:CDS	35.0%
	AAACCTGTTACGACTCGTAT+AGG	+	chr3.2:81898816-81898835	MS.gene013193:CDS	40.0%
	AACCTGTTACGACTCGTATA+GGG	+	chr3.2:81898817-81898836	MS.gene013193:CDS	40.0%
	AGGTTTCAGATTCAGCAGAT+AGG	-	chr3.2:81898179-81898198	None:intergenic	40.0%
	ATAATAGTTACGCGTTTGCC+AGG	-	chr3.2:81898297-81898316	None:intergenic	40.0%
	ATCCTAGATCACTGCTTGAT+CGG	+	chr3.2:81898204-81898223	MS.gene013193:CDS	40.0%
	CAGATTCAGCAGATAGGTTA+AGG	-	chr3.2:81898173-81898192	None:intergenic	40.0%
	CGAGTCCAATTCAAACAAGT+CGG	-	chr3.2:81898763-81898782	None:intergenic	40.0%
	GAGTCCAATTCAAACAAGTC+GGG	-	chr3.2:81898762-81898781	None:intergenic	40.0%
	TGACATCAATCCAGACTTTG+AGG	-	chr3.2:81898608-81898627	None:intergenic	40.0%
	TTTGGACGATCGTGAAATCA+AGG	+	chr3.2:81898233-81898252	MS.gene013193:CDS	40.0%
!	ATCAAGCAGTGATCTAGGAT+AGG	-	chr3.2:81898204-81898223	None:intergenic	40.0%
!	CGACTTGTTTGAATTGGACT+CGG	+	chr3.2:81898761-81898780	MS.gene013193:CDS	40.0%
!	GCTTGGAAGTTACTTTGATG+CGG	-	chr3.2:81898891-81898910	None:intergenic	40.0%
!!!	ACTTTCCACTCCTTTTTCAG+CGG	-	chr3.2:81898636-81898655	None:intergenic	40.0%
!!!	CGACAACGTTGTTTTTCTTG+AGG	+	chr3.2:81899202-81899221	MS.gene013193:CDS	40.0%
!!!	GGTGTTTTTTGTTACAGGTG+TGG	+	chr3.2:81898708-81898727	MS.gene013193:CDS	40.0%
!!!	GTTGTTTTTCTTGAGGATGC+TGG	+	chr3.2:81899209-81899228	MS.gene013193:CDS	40.0%
	ACGACCCTGCAGTAAATTTG+AGG	-	chr3.2:81899120-81899139	None:intergenic	45.0%
	ATAACCAAGGACCCTCATCA+AGG	-	chr3.2:81898380-81898399	None:intergenic	45.0%
	ATGCTTCCTTCTCCATCTAC+AGG	+	chr3.2:81899086-81899105	MS.gene013193:CDS	45.0%
	CAACATTGACGGTCTTACCT+TGG	+	chr3.2:81898131-81898150	MS.gene013193:CDS	45.0%
	CAGTGTCTACAGCAAGCATA+CGG	-	chr3.2:81898977-81898996	None:intergenic	45.0%
	CCCACACAACTCAACATTGA+CGG	+	chr3.2:81898120-81898139	MS.gene013193:CDS	45.0%
	GACGATGGAGTACGTAATTG+GGG	+	chr3.2:81898783-81898802	MS.gene013193:CDS	45.0%
	GCGCATATAGGAGCTATGAA+TGG	-	chr3.2:81898586-81898605	None:intergenic	45.0%
	GGACATTCGTTCACTGAAGT+AGG	-	chr3.2:81898262-81898281	None:intergenic	45.0%
	GGACGATGGAGTACGTAATT+GGG	+	chr3.2:81898782-81898801	MS.gene013193:CDS	45.0%
	GGTACTCTCCTAACCTATGA+TGG	+	chr3.2:81898552-81898571	MS.gene013193:CDS	45.0%
	TCATTTGGATATCTGGGTGC+AGG	+	chr3.2:81898467-81898486	MS.gene013193:CDS	45.0%
	TCGGCCTCAAATTTACTGCA+GGG	+	chr3.2:81899113-81899132	MS.gene013193:CDS	45.0%
	TGCTACAAACAGATGGCTTC+AGG	+	chr3.2:81898845-81898864	MS.gene013193:CDS	45.0%
	TGGAAAGTTCGTGCTAGCAA+TGG	+	chr3.2:81898648-81898667	MS.gene013193:CDS	45.0%
	TGTCACTGTACCGCTGAAAA+AGG	+	chr3.2:81898623-81898642	MS.gene013193:CDS	45.0%
	TGTTACAGGTGTGGCATTCT+CGG	+	chr3.2:81898717-81898736	MS.gene013193:CDS	45.0%
	TGTTACGACTCGTATAGGGT+CGG	+	chr3.2:81898821-81898840	MS.gene013193:CDS	45.0%
	TGTTGCGACCATCATAGGTT+AGG	-	chr3.2:81898563-81898582	None:intergenic	45.0%
	TTCTCCATCTACAGGAGTCT+CGG	+	chr3.2:81899094-81899113	MS.gene013193:CDS	45.0%
	TTTACTGCAGGGTCGTTCTA+TGG	+	chr3.2:81899124-81899143	MS.gene013193:CDS	45.0%
	TTTGAATTGGACTCGGACGA+TGG	+	chr3.2:81898768-81898787	MS.gene013193:CDS	45.0%
	TTTGATGCGGCACAACATCA+GGG	-	chr3.2:81898878-81898897	None:intergenic	45.0%
!	ACCGTCAATGTTGAGTTGTG+TGG	-	chr3.2:81898124-81898143	None:intergenic	45.0%
!	CCGTCAATGTTGAGTTGTGT+GGG	-	chr3.2:81898123-81898142	None:intergenic	45.0%
!	GTAGATGGAGAAGGAAGCAT+CGG	-	chr3.2:81899086-81899105	None:intergenic	45.0%
!	GTGTCCCGACTTGTTTGAAT+TGG	+	chr3.2:81898755-81898774	MS.gene013193:CDS	45.0%
!	TCAAAGGTTGGTCAAGGCAT+TGG	+	chr3.2:81898519-81898538	MS.gene013193:CDS	45.0%
!!	AATGGTGTTGCGACCATCAT+AGG	-	chr3.2:81898568-81898587	None:intergenic	45.0%
	ACCCTCATCAAGGATACGAG+CGG	-	chr3.2:81898370-81898389	None:intergenic	50.0%
	CAGAATTGTCAGCATGGGTG+CGG	-	chr3.2:81898929-81898948	None:intergenic	50.0%
	CAGGGTCGTTCTATGGTACT+TGG	+	chr3.2:81899131-81899150	MS.gene013193:CDS	50.0%
	CATTCTCGGACATTCGGACA+AGG	+	chr3.2:81898731-81898750	MS.gene013193:CDS	50.0%
	CCTATATGCGCCTCAAAGTC+TGG	+	chr3.2:81898595-81898614	MS.gene013193:CDS	50.0%
	CGGACGATGGAGTACGTAAT+TGG	+	chr3.2:81898781-81898800	MS.gene013193:CDS	50.0%
	CTCGGCCTCAAATTTACTGC+AGG	+	chr3.2:81899112-81899131	MS.gene013193:CDS	50.0%
	CTGTACCGCTGAAAAAGGAG+TGG	+	chr3.2:81898628-81898647	MS.gene013193:CDS	50.0%
	CTTTGATGCGGCACAACATC+AGG	-	chr3.2:81898879-81898898	None:intergenic	50.0%
	GACCCTATACGAGTCGTAAC+AGG	-	chr3.2:81898822-81898841	None:intergenic	50.0%
	GACCGATCAAGCAGTGATCT+AGG	-	chr3.2:81898209-81898228	None:intergenic	50.0%
	GAGACTCCTGTAGATGGAGA+AGG	-	chr3.2:81899095-81899114	None:intergenic	50.0%
	GATATCTGGGTGCAGGTCTA+TGG	+	chr3.2:81898474-81898493	MS.gene013193:CDS	50.0%
	GCAAAAGTGAAAGGAGCAGC+AGG	-	chr3.2:81899161-81899180	None:intergenic	50.0%
	GTATCCTTGATGAGGGTCCT+TGG	+	chr3.2:81898373-81898392	MS.gene013193:CDS	50.0%
	GTGTGGCATTCTCGGACATT+CGG	+	chr3.2:81898725-81898744	MS.gene013193:CDS	50.0%
	TTCCAAGCCAGTTCTTCTGC+TGG	+	chr3.2:81898903-81898922	MS.gene013193:CDS	50.0%
!	CAGGTCTATGGATTGCCCTT+TGG	+	chr3.2:81898486-81898505	MS.gene013193:CDS	50.0%
!	CATCAAGCTTGTGGTGGAAC+TGG	-	chr3.2:81898347-81898366	None:intergenic	50.0%
!	CTGCTTGATCGGTCGTGTTT+TGG	+	chr3.2:81898215-81898234	MS.gene013193:CDS	50.0%
!	GCAGTGATCTAGGATAGGAG+AGG	-	chr3.2:81898199-81898218	None:intergenic	50.0%
!!	CAAGGCATTGGCAGCTTTCT+CGG	+	chr3.2:81898531-81898550	MS.gene013193:CDS	50.0%
!!	CCAGACTTTGAGGCGCATAT+AGG	-	chr3.2:81898598-81898617	None:intergenic	50.0%
!!	GCTGCTCCTTTCACTTTTGC+TGG	+	chr3.2:81899161-81899180	MS.gene013193:CDS	50.0%
	ACAGTGATCGGAACCACACC+AGG	-	chr3.2:81898438-81898457	None:intergenic	55.0%
	ATGCGGCACAACATCAGGGA+TGG	-	chr3.2:81898874-81898893	None:intergenic	55.0%
	GCCGCTCGTATCCTTGATGA+GGG	+	chr3.2:81898366-81898385	MS.gene013193:CDS	55.0%
	GGTCAAACCTTCGTCAGCCA+AGG	-	chr3.2:81898151-81898170	None:intergenic	55.0%
	GGTCGGCTGCTACAAACAGA+TGG	+	chr3.2:81898838-81898857	MS.gene013193:CDS	55.0%
	GGTCTTACCTTGGCTGACGA+AGG	+	chr3.2:81898141-81898160	MS.gene013193:CDS	55.0%
	GTCATGGAACGCATTGCTCC+TGG	+	chr3.2:81898417-81898436	MS.gene013193:CDS	55.0%
	GTTTGCCAGGTTTCCACGCA+CGG	-	chr3.2:81898284-81898303	None:intergenic	55.0%
	TACGAGCGGCATCAAGCTTG+TGG	-	chr3.2:81898356-81898375	None:intergenic	55.0%
	TGCCGCTCGTATCCTTGATG+AGG	+	chr3.2:81898365-81898384	MS.gene013193:CDS	55.0%
	TGCGGCACAACATCAGGGAT+GGG	-	chr3.2:81898873-81898892	None:intergenic	55.0%
	TTTGCCAGGTTTCCACGCAC+GGG	-	chr3.2:81898283-81898302	None:intergenic	55.0%
	ATGTCCCGTGCGTGGAAACC+TGG	+	chr3.2:81898276-81898295	MS.gene013193:CDS	60.0%
	GAGCGGCATCAAGCTTGTGG+TGG	-	chr3.2:81898353-81898372	None:intergenic	60.0%
	GAGGCCGAGACTCCTGTAGA+TGG	-	chr3.2:81899101-81899120	None:intergenic	60.0%
	GGAACGCATTGCTCCTGGTG+TGG	+	chr3.2:81898422-81898441	MS.gene013193:CDS	60.0%
	GGCCAGCAGAAGAACTGGCT+TGG	-	chr3.2:81898908-81898927	None:intergenic	60.0%
	GTGAACGAATGTCCCGTGCG+TGG	+	chr3.2:81898268-81898287	MS.gene013193:CDS	60.0%
	GCAGAGCAGTCTGAGCAGCG+AGG	-	chr3.2:81899025-81899044	None:intergenic	65.0%
	GGTGCGGCCAGCAGAAGAAC+TGG	-	chr3.2:81898913-81898932	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	81898114	81899271	81898114	ID=MS.gene013193
chr3.2	mRNA	81898114	81899271	81898114	ID=MS.gene013193.t1;Parent=MS.gene013193
chr3.2	exon	81898114	81899271	81898114	ID=MS.gene013193.t1.exon1;Parent=MS.gene013193.t1
chr3.2	CDS	81898114	81899271	81898114	ID=cds.MS.gene013193.t1;Parent=MS.gene013193.t1

Gene Sequence

>MS.gene013193

ATGGATCCCACACAACTCAACATTGACGGTCTTACCTTGGCTGACGAAGGTTTGACCCTTAACCTATCTGCTGAATCTGAAACCTCTCCTATCCTAGATCACTGCTTGATCGGTCGTGTTTTGGACGATCGTGAAATCAAGGCAGCCTACTTCAGTGAACGAATGTCCCGTGCGTGGAAACCTGGCAAACGCGTAACTATTATCAAATCTGATGATGATCGCTTCTTGTTCCAGTTCCACCACAAGCTTGATGCCGCTCGTATCCTTGATGAGGGTCCTTGGTTATATGATAACTTTCATATAGTCATGGAACGCATTGCTCCTGGTGTGGTTCCGATCACTGTTCCTCTAATTCATTTGGATATCTGGGTGCAGGTCTATGGATTGCCCTTTGGTTTCATTCAATCAAAGGTTGGTCAAGGCATTGGCAGCTTTCTCGGTACTCTCCTAACCTATGATGGTCGCAACACCATTCATAGCTCCTATATGCGCCTCAAAGTCTGGATTGATGTCACTGTACCGCTGAAAAAGGAGTGGAAAGTTCGTGCTAGCAATGGTTCCTTTGTTACTATTTTTTTCAAATATGAGAAACTGGGTGTTTTTTGTTACAGGTGTGGCATTCTCGGACATTCGGACAAGGTGTGTCCCGACTTGTTTGAATTGGACTCGGACGATGGAGTACGTAATTGGGGACTTGATCTCAAACCTGTTACGACTCGTATAGGGTCGGCTGCTACAAACAGATGGCTTCAGGATCCCATCCCTGATGTTGTGCCGCATCAAAGTAACTTCCAAGCCAGTTCTTCTGCTGGCCGCACCCATGCTGACAATTCTGTTTTGAATTTTAATAACTTTAATAACCGTATGCTTGCTGTAGACACTGCTATTAATGCTATTAAACAGGATGTCCTCGCTGCTCAGACTGCTCTGCAAGCTAAAAATGTCACAAATGGTGCTGGTTGTCAAGTTCCGATGCTTCCTTCTCCATCTACAGGAGTCTCGGCCTCAAATTTACTGCAGGGTCGTTCTATGGTACTTGGTTTGCCTGCTGCTCCTTTCACTTTTGCTGGTACAAATAATACTCAAAGCGACAACGTTGTTTTTCTTGAGGATGCTGGTTCTGACTTGAAGAAACGCAAGTGTATGCTCGCCCTTTAG

Protein sequence

>MS.gene013193.t1

MDPTQLNIDGLTLADEGLTLNLSAESETSPILDHCLIGRVLDDREIKAAYFSERMSRAWKPGKRVTIIKSDDDRFLFQFHHKLDAARILDEGPWLYDNFHIVMERIAPGVVPITVPLIHLDIWVQVYGLPFGFIQSKVGQGIGSFLGTLLTYDGRNTIHSSYMRLKVWIDVTVPLKKEWKVRASNGSFVTIFFKYEKLGVFCYRCGILGHSDKVCPDLFELDSDDGVRNWGLDLKPVTTRIGSAATNRWLQDPIPDVVPHQSNFQASSSAGRTHADNSVLNFNNFNNRMLAVDTAINAIKQDVLAAQTALQAKNVTNGAGCQVPMLPSPSTGVSASNLLQGRSMVLGLPAAPFTFAGTNNTQSDNVVFLEDAGSDLKKRKCMLAL