Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08708.t1 | XP_024630605.1 | 54 | 274 | 118 | 3 | 30 | 303 | 48 | 313 | 6.90E-79 | 304.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08708.t1 | O04244 | 27.2 | 136 | 95 | 2 | 29 | 162 | 6 | 139 | 4.1e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08708.t1 | G7J017 | 56.2 | 292 | 62 | 3 | 1 | 292 | 55 | 280 | 4.7e-77 | 297.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08708.t1 | MTR_3g053920 | 54.795 | 292 | 66 | 3 | 1 | 292 | 55 | 280 | 1.45e-95 | 285 |
| MS.gene08708.t1 | MTR_4g103530 | 84.946 | 93 | 14 | 0 | 1 | 93 | 1 | 93 | 2.43e-55 | 179 |
| MS.gene08708.t1 | MTR_2g061500 | 84.000 | 75 | 12 | 0 | 1 | 75 | 55 | 129 | 8.94e-43 | 145 |
| MS.gene08708.t1 | MTR_7g011710 | 67.059 | 85 | 28 | 0 | 1 | 85 | 1 | 85 | 1.55e-37 | 132 |
| MS.gene08708.t1 | MTR_6g033215 | 34.965 | 143 | 91 | 1 | 5 | 145 | 152 | 294 | 1.14e-26 | 107 |
| MS.gene08708.t1 | MTR_0010s0370 | 37.500 | 128 | 78 | 1 | 5 | 130 | 57 | 184 | 5.48e-25 | 100 |
| MS.gene08708.t1 | MTR_0010s0350 | 37.500 | 128 | 78 | 1 | 5 | 130 | 57 | 184 | 5.48e-25 | 100 |
| MS.gene08708.t1 | MTR_8g463110 | 53.846 | 91 | 38 | 1 | 235 | 325 | 1 | 87 | 1.55e-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08708.t1 | AT2G17920 | 26.667 | 165 | 115 | 4 | 1 | 162 | 58 | 219 | 1.89e-16 | 79.0 |
| MS.gene08708.t1 | AT3G57587 | 26.761 | 142 | 100 | 2 | 23 | 162 | 80 | 219 | 4.96e-16 | 78.6 |
| MS.gene08708.t1 | AT2G41590 | 26.761 | 142 | 100 | 2 | 23 | 162 | 80 | 219 | 2.37e-15 | 76.6 |
| MS.gene08708.t1 | AT2G13450 | 26.220 | 164 | 115 | 4 | 1 | 161 | 58 | 218 | 6.97e-15 | 75.5 |
| MS.gene08708.t1 | AT5G36228 | 26.627 | 169 | 118 | 3 | 8 | 172 | 63 | 229 | 1.01e-14 | 74.7 |
| MS.gene08708.t1 | AT3G31430 | 30.061 | 163 | 108 | 4 | 1 | 161 | 153 | 311 | 2.48e-14 | 73.2 |
| MS.gene08708.t1 | AT4G02000 | 27.206 | 136 | 95 | 2 | 29 | 162 | 6 | 139 | 9.55e-14 | 71.2 |
| MS.gene08708.t1 | AT3G47920 | 26.761 | 142 | 100 | 2 | 23 | 162 | 9 | 148 | 1.49e-13 | 70.5 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATATCGAGTTCTGGATTC+AGG | 0.286325 | 4.4:-44479350 | MS.gene08708:CDS |
| CTGATGATGGAGTCCGTAAC+TGG | 0.286721 | 4.4:-44479036 | MS.gene08708:CDS |
| ATTAAAATTTGCATTATTTC+CGG | 0.298144 | 4.4:+44478868 | None:intergenic |
| TGCTACTAACCGTTGGTTAC+AGG | 0.298585 | 4.4:-44478972 | MS.gene08708:CDS |
| CCTAAGGTAGGTCAAGGTAT+AGG | 0.317348 | 4.4:-44479298 | MS.gene08708:CDS |
| CGCTGCCCAAAATGCAGTTT+TGG | 0.326734 | 4.4:-44478792 | MS.gene08708:CDS |
| CATCGACCTTATGGTGGAAT+TGG | 0.330471 | 4.4:+44479473 | None:intergenic |
| TGATAACTTTCATATTGTTA+TGG | 0.330634 | 4.4:-44479416 | MS.gene08708:CDS |
| CAGGTTCACGGTTTACCATT+TGG | 0.350945 | 4.4:-44479331 | MS.gene08708:CDS |
| CGTTGAATCATATCGAGTTC+TGG | 0.362336 | 4.4:-44479357 | MS.gene08708:CDS |
| CCCTGTCTTGCAACTCGTCC+TGG | 0.368175 | 4.4:+44470397 | None:intergenic |
| GCCAGGACGAGTTGCAAGAC+AGG | 0.368471 | 4.4:-44470398 | MS.gene08708:CDS |
| ACGCGCCATGCTTGTTTCAA+TGG | 0.368758 | 4.4:+44479184 | None:intergenic |
| ACAAAACTCGGGACAACACC+TGG | 0.379429 | 4.4:+44479382 | None:intergenic |
| TGCGACATGGTGGCAGGAAT+AGG | 0.384544 | 4.4:+44478947 | None:intergenic |
| CACGGGCAACATCGACCTTA+TGG | 0.384826 | 4.4:+44479464 | None:intergenic |
| CTAAGGTAGGTCAAGGTATA+GGG | 0.394084 | 4.4:-44479297 | MS.gene08708:CDS |
| TGCGACCATCATAGACTTTG+AGG | 0.401247 | 4.4:+44479260 | None:intergenic |
| GCGACATGGTGGCAGGAATA+GGG | 0.413660 | 4.4:+44478948 | None:intergenic |
| CACACAGCTGCTGCTCCTAC+TGG | 0.415786 | 4.4:-44478914 | MS.gene08708:CDS |
| GTAGCGATCAGCCACAGATT+TGG | 0.416977 | 4.4:+44479501 | None:intergenic |
| TGGCTTGAACCCAATGTGTC+AGG | 0.420239 | 4.4:+44470417 | None:intergenic |
| TTAGGTGCGGTCCCCAGTTA+CGG | 0.427820 | 4.4:+44479023 | None:intergenic |
| AAGCCTACCTCACAACGAAT+TGG | 0.429260 | 4.4:-44479001 | MS.gene08708:CDS |
| TGTCACAGGTGTGGATTGTT+GGG | 0.429577 | 4.4:-44479100 | MS.gene08708:CDS |
| TTCGTTGTGAGGTAGGCTTT+AGG | 0.434179 | 4.4:+44479005 | None:intergenic |
| TGTTTGCAAGGTGCATCTGC+TGG | 0.439710 | 4.4:-44478744 | MS.gene08708:CDS |
| CCAGACCGTCCGTTGGTGCT+CGG | 0.442939 | 4.4:-44478683 | MS.gene08708:CDS |
| TTGTCACAGGTGTGGATTGT+TGG | 0.444024 | 4.4:-44479101 | MS.gene08708:CDS |
| CCTATACCTTGACCTACCTT+AGG | 0.447996 | 4.4:+44479298 | None:intergenic |
| TGCATGGAAGCCAGGCAAAA+AGG | 0.450638 | 4.4:-44479533 | MS.gene08708:CDS |
| CCGAGCACCAACGGACGGTC+TGG | 0.452842 | 4.4:+44478683 | None:intergenic |
| TTTGAGCTTGAATCTGATGA+TGG | 0.454699 | 4.4:-44479049 | MS.gene08708:CDS |
| TACCTCAGCAGCGGATTCAC+TGG | 0.456881 | 4.4:+44478640 | None:intergenic |
| GATTCAAGCTCAAACAAGTC+CGG | 0.474211 | 4.4:+44479058 | None:intergenic |
| CAGCGGATTCACTGGAGAAT+GGG | 0.475573 | 4.4:+44478648 | None:intergenic |
| AGAACTCGATATGATTCAAC+GGG | 0.480591 | 4.4:+44479359 | None:intergenic |
| TGCAAGACAGGGAGCGAGGA+AGG | 0.481784 | 4.4:-44470386 | MS.gene08708:CDS |
| GCAGCGGATTCACTGGAGAA+TGG | 0.484401 | 4.4:+44478647 | None:intergenic |
| GTTTGTTGCGACATGGTGGC+AGG | 0.491404 | 4.4:+44478941 | None:intergenic |
| CTTGTTCCAATTCCACCATA+AGG | 0.494253 | 4.4:-44479479 | MS.gene08708:CDS |
| GTTGCCCGTGTTCTTGATGA+AGG | 0.499170 | 4.4:-44479451 | MS.gene08708:CDS |
| TGCCTTCAGCAGTAGTGGTA+CGG | 0.503584 | 4.4:+44478891 | None:intergenic |
| CAGGTCAGACCTGACACATT+GGG | 0.504029 | 4.4:-44470426 | MS.gene08708:CDS |
| CTGCTGGCCTCTTCCTCCAC+AGG | 0.506951 | 4.4:-44478728 | MS.gene08708:CDS |
| TGTTGTACCTGTGGAGGAAG+AGG | 0.512440 | 4.4:+44478721 | None:intergenic |
| TGATTCAACGGGACAAAACT+CGG | 0.513941 | 4.4:+44479370 | None:intergenic |
| TTAGGTTGAATGAAACCAAA+TGG | 0.516817 | 4.4:+44479316 | None:intergenic |
| ATGTCTCGTGCATGGAAGCC+AGG | 0.520191 | 4.4:-44479541 | MS.gene08708:CDS |
| GTGCCAATTCGTTGTGAGGT+AGG | 0.526260 | 4.4:+44478998 | None:intergenic |
| ACATACTGACAAGGTGTGCC+CGG | 0.527437 | 4.4:-44479077 | MS.gene08708:CDS |
| ATTTGGTTTCATTCAACCTA+AGG | 0.529018 | 4.4:-44479314 | MS.gene08708:CDS |
| GAGTTCTGGATTCAGGTTCA+CGG | 0.531069 | 4.4:-44479343 | MS.gene08708:CDS |
| AACATTAACAAAATCTCCAT+TGG | 0.531836 | 4.4:+44479153 | None:intergenic |
| GGTAACCTCAAAGTCTATGA+TGG | 0.533933 | 4.4:-44479265 | MS.gene08708:CDS |
| TGAGTCTCATATAAGAGCTA+TGG | 0.541141 | 4.4:+44479230 | None:intergenic |
| GTGTTCTTGATGAAGGTCCA+TGG | 0.544068 | 4.4:-44479444 | MS.gene08708:CDS |
| TCCGGTGCCTTCAGCAGTAG+TGG | 0.545510 | 4.4:+44478886 | None:intergenic |
| TGACACATTGGGTTCAAGCC+AGG | 0.546498 | 4.4:-44470415 | MS.gene08708:CDS |
| GCAGGTAAACCGAGCACCAA+CGG | 0.548004 | 4.4:+44478674 | None:intergenic |
| GCAGTAGTGGTACGGCCAGT+AGG | 0.550699 | 4.4:+44478899 | None:intergenic |
| TGATGATGGAGTCCGTAACT+GGG | 0.552518 | 4.4:-44479035 | MS.gene08708:CDS |
| ATTCAAGCTCAAACAAGTCC+GGG | 0.553322 | 4.4:+44479059 | None:intergenic |
| TCACTGGAGAATGGGACAGC+AGG | 0.556511 | 4.4:+44478656 | None:intergenic |
| CAAAACTCGGGACAACACCT+GGG | 0.560106 | 4.4:+44479383 | None:intergenic |
| TGGCGCGTTCGTGCTACCAA+TGG | 0.564084 | 4.4:-44479169 | MS.gene08708:CDS |
| GATTCAACGGGACAAAACTC+GGG | 0.564434 | 4.4:+44479371 | None:intergenic |
| GGTTTCATTCAACCTAAGGT+AGG | 0.570176 | 4.4:-44479310 | MS.gene08708:CDS |
| ACCACTACTGCTGAAGGCAC+CGG | 0.574813 | 4.4:-44478887 | MS.gene08708:CDS |
| ATTCAACCTAAGGTAGGTCA+AGG | 0.576603 | 4.4:-44479304 | MS.gene08708:CDS |
| TCAGGTCAGACCTGACACAT+TGG | 0.577432 | 4.4:-44470427 | MS.gene08708:intron |
| TGGTGTGAATTCTGTTTGCA+AGG | 0.587679 | 4.4:-44478756 | MS.gene08708:CDS |
| GTGAGGAGTTTGTTGCGACA+TGG | 0.591303 | 4.4:+44478934 | None:intergenic |
| GTTATGGATCGTATCTCCCC+AGG | 0.592035 | 4.4:-44479400 | MS.gene08708:CDS |
| CTCCAGTGAATCCGCTGCTG+AGG | 0.592676 | 4.4:-44478642 | MS.gene08708:intron |
| GTAGGAGCAGCAGCTGTGTG+AGG | 0.592777 | 4.4:+44478917 | None:intergenic |
| GCACCGCTGCTACTAACCGT+TGG | 0.593722 | 4.4:-44478979 | MS.gene08708:CDS |
| CCAGGACGAGTTGCAAGACA+GGG | 0.594095 | 4.4:-44470397 | MS.gene08708:CDS |
| TCTGCTTCCAGACCGTCCGT+TGG | 0.599029 | 4.4:-44478690 | MS.gene08708:CDS |
| TTCCTCTTGTACCTCAGCAG+CGG | 0.602236 | 4.4:+44478631 | None:intergenic |
| GCTAGCGTATGTCTCGTGCA+TGG | 0.612617 | 4.4:-44479549 | None:intergenic |
| GAGTTGCAAGACAGGGAGCG+AGG | 0.613309 | 4.4:-44470390 | MS.gene08708:CDS |
| TGAAAGTTATCATAAAGCCA+TGG | 0.613605 | 4.4:+44479427 | None:intergenic |
| ACTAGAAGCTGTTGTACCTG+TGG | 0.618260 | 4.4:+44478712 | None:intergenic |
| CAGAACTCGATATGATTCAA+CGG | 0.618989 | 4.4:+44479358 | None:intergenic |
| AGAAGCTGTTGTACCTGTGG+AGG | 0.623461 | 4.4:+44478715 | None:intergenic |
| GGCCGTACCACTACTGCTGA+AGG | 0.624051 | 4.4:-44478893 | MS.gene08708:CDS |
| AGCGGTGCCAATTCGTTGTG+AGG | 0.624880 | 4.4:+44478994 | None:intergenic |
| CTGTTCCATTGAAACAAGCA+TGG | 0.630333 | 4.4:-44479189 | MS.gene08708:CDS |
| GGGCAACATCGACCTTATGG+TGG | 0.637867 | 4.4:+44479467 | None:intergenic |
| TAACCAACGGTTAGTAGCAG+CGG | 0.648690 | 4.4:+44478976 | None:intergenic |
| GTAAACCGAGCACCAACGGA+CGG | 0.660647 | 4.4:+44478678 | None:intergenic |
| TGGACCTTCATCAAGAACAC+GGG | 0.663937 | 4.4:+44479447 | None:intergenic |
| ATGGACCTTCATCAAGAACA+CGG | 0.668470 | 4.4:+44479446 | None:intergenic |
| GGTCACGATCACCAAATCTG+TGG | 0.672214 | 4.4:-44479512 | MS.gene08708:CDS |
| GATGATGGAGTCCGTAACTG+GGG | 0.673105 | 4.4:-44479034 | MS.gene08708:CDS |
| GAATAGGGTCCTGTAACCAA+CGG | 0.675018 | 4.4:+44478963 | None:intergenic |
| GAGACTGAAAAGCAAGCACA+CGG | 0.683711 | 4.4:+44478843 | None:intergenic |
| TGTGAGGTAGGCTTTAGGTG+CGG | 0.691051 | 4.4:+44479010 | None:intergenic |
| AAAACTCGGGACAACACCTG+GGG | 0.694917 | 4.4:+44479384 | None:intergenic |
| AGGAGTTTGTTGCGACATGG+TGG | 0.709977 | 4.4:+44478937 | None:intergenic |
| ATTGTTGGGACATACTGACA+AGG | 0.821038 | 4.4:-44479086 | MS.gene08708:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 44470371 | 44479563 | 44470371 | ID=MS.gene08708 |
| chr4.4 | mRNA | 44470371 | 44479563 | 44470371 | ID=MS.gene08708.t1;Parent=MS.gene08708 |
| chr4.4 | exon | 44478643 | 44479563 | 44478643 | ID=MS.gene08708.t1.exon1;Parent=MS.gene08708.t1 |
| chr4.4 | CDS | 44478643 | 44479563 | 44478643 | ID=cds.MS.gene08708.t1;Parent=MS.gene08708.t1 |
| chr4.4 | exon | 44470371 | 44470445 | 44470371 | ID=MS.gene08708.t1.exon2;Parent=MS.gene08708.t1 |
| chr4.4 | CDS | 44470371 | 44470445 | 44470371 | ID=cds.MS.gene08708.t1;Parent=MS.gene08708.t1 |
| Gene Sequence |
| Protein sequence |
