AlfalfaGEDB | MS.gene22730

M. sativa cultivar XinJiangDaYe / MS.gene22730

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22730.t1	AES70414.1	69	290	61	4	1	277	1	274	1.80E-101	379.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22730.t1	G7J017	69.0	290	61	4	1	277	1	274	1.3e-101	379.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene26038	MS.gene22730	PPI
MS.gene22730	MS.gene08713	PPI
MS.gene85308	MS.gene22730	PPI
MS.gene22730	MS.gene09517	PPI
MS.gene22730	MS.gene87845	PPI
MS.gene073573	MS.gene22730	PPI
MS.gene031967	MS.gene22730	PPI
MS.gene34812	MS.gene22730	PPI
MS.gene02896	MS.gene22730	PPI
MS.gene22730	MS.gene62249	PPI
MS.gene063214	MS.gene22730	PPI
MS.gene55682	MS.gene22730	PPI
MS.gene51799	MS.gene22730	PPI
MS.gene035626	MS.gene22730	PPI
MS.gene067340	MS.gene22730	PPI
MS.gene65623	MS.gene22730	PPI
MS.gene71675	MS.gene22730	PPI
MS.gene22730	MS.gene044729	PPI
MS.gene019177	MS.gene22730	PPI
MS.gene69495	MS.gene22730	PPI
MS.gene049512	MS.gene22730	PPI
MS.gene29406	MS.gene22730	PPI
MS.gene22730	MS.gene60390	PPI
MS.gene038623	MS.gene22730	PPI
MS.gene22730	MS.gene039692	PPI
MS.gene05357	MS.gene22730	PPI
MS.gene22730	MS.gene027169	PPI
MS.gene032311	MS.gene22730	PPI
MS.gene22730	MS.gene019177	PPI
MS.gene004417	MS.gene22730	PPI
MS.gene86849	MS.gene22730	PPI
MS.gene50392	MS.gene22730	PPI
MS.gene072852	MS.gene22730	PPI
MS.gene22730	MS.gene024297	PPI
MS.gene39956	MS.gene22730	PPI
MS.gene22730	MS.gene057934	PPI
MS.gene22730	MS.gene37537	PPI
MS.gene021968	MS.gene22730	PPI
MS.gene22730	MS.gene024841	PPI
MS.gene22730	MS.gene069786	PPI
MS.gene09589	MS.gene22730	PPI
MS.gene22730	MS.gene42010	PPI
MS.gene22730	MS.gene025158	PPI
MS.gene22730	MS.gene27329	PPI
MS.gene43393	MS.gene22730	PPI
MS.gene22730	MS.gene41393	PPI
MS.gene057828	MS.gene22730	PPI
MS.gene34197	MS.gene22730	PPI
MS.gene22730	MS.gene016632	PPI
MS.gene66650	MS.gene22730	PPI
MS.gene005120	MS.gene22730	PPI
MS.gene22730	MS.gene40469	PPI
MS.gene048072	MS.gene22730	PPI
MS.gene060386	MS.gene22730	PPI
MS.gene71588	MS.gene22730	PPI
MS.gene04749	MS.gene22730	PPI
MS.gene05436	MS.gene22730	PPI
MS.gene22730	MS.gene03149	PPI
MS.gene059736	MS.gene22730	PPI
MS.gene70932	MS.gene22730	PPI
MS.gene027169	MS.gene22730	PPI
MS.gene22730	MS.gene29321	PPI
MS.gene021965	MS.gene22730	PPI
MS.gene055683	MS.gene22730	PPI
MS.gene042614	MS.gene22730	PPI
MS.gene41643	MS.gene22730	PPI
MS.gene26844	MS.gene22730	PPI
MS.gene021555	MS.gene22730	PPI
MS.gene021647	MS.gene22730	PPI
MS.gene22730	MS.gene34618	PPI
MS.gene22730	MS.gene59460	PPI
MS.gene22730	MS.gene81194	PPI
MS.gene019165	MS.gene22730	PPI
MS.gene22730	MS.gene060773	PPI
MS.gene048706	MS.gene22730	PPI
MS.gene22730	MS.gene038394	PPI
MS.gene009121	MS.gene22730	PPI
MS.gene94654	MS.gene22730	PPI
MS.gene22730	MS.gene019001	PPI
MS.gene51804	MS.gene22730	PPI
MS.gene22730	MS.gene53904	PPI
MS.gene87845	MS.gene22730	PPI
MS.gene36069	MS.gene22730	PPI
MS.gene22730	MS.gene24070	PPI
MS.gene22730	MS.gene73261	PPI
MS.gene97410	MS.gene22730	PPI
MS.gene83772	MS.gene22730	PPI
MS.gene32415	MS.gene22730	PPI
MS.gene22730	MS.gene055546	PPI
MS.gene009213	MS.gene22730	PPI
MS.gene008927	MS.gene22730	PPI
MS.gene22730	MS.gene55209	PPI
MS.gene045614	MS.gene22730	PPI
MS.gene049561	MS.gene22730	PPI
MS.gene63762	MS.gene22730	PPI
MS.gene22730	MS.gene44144	PPI
MS.gene30208	MS.gene22730	PPI
MS.gene028716	MS.gene22730	PPI
MS.gene025873	MS.gene22730	PPI
MS.gene06164	MS.gene22730	PPI
MS.gene013676	MS.gene22730	PPI
MS.gene55209	MS.gene22730	PPI
MS.gene05358	MS.gene22730	PPI
MS.gene22730	MS.gene068629	PPI
MS.gene22730	MS.gene004417	PPI
MS.gene64178	MS.gene22730	PPI
MS.gene22730	MS.gene009781	PPI
MS.gene030090	MS.gene22730	PPI
MS.gene95561	MS.gene22730	PPI
MS.gene96174	MS.gene22730	PPI
MS.gene018196	MS.gene22730	PPI
MS.gene065627	MS.gene22730	PPI
MS.gene57019	MS.gene22730	PPI
MS.gene018745	MS.gene22730	PPI
MS.gene22730	MS.gene21066	PPI
MS.gene65546	MS.gene22730	PPI
MS.gene22730	MS.gene009213	PPI
MS.gene22730	MS.gene013890	PPI
MS.gene22730	MS.gene98127	PPI
MS.gene000506	MS.gene22730	PPI
MS.gene22730	MS.gene003264	PPI
MS.gene03936	MS.gene22730	PPI
MS.gene038098	MS.gene22730	PPI
MS.gene60059	MS.gene22730	PPI
MS.gene22111	MS.gene22730	PPI
MS.gene28545	MS.gene22730	PPI
MS.gene22730	MS.gene49057	PPI
MS.gene28718	MS.gene22730	PPI
MS.gene055560	MS.gene22730	PPI
MS.gene22730	MS.gene89815	PPI
MS.gene008275	MS.gene22730	PPI
MS.gene22730	MS.gene018474	PPI
MS.gene22730	MS.gene048088	PPI
MS.gene22730	MS.gene86868	PPI
MS.gene038757	MS.gene22730	PPI
MS.gene064966	MS.gene22730	PPI
MS.gene22730	MS.gene96163	PPI
MS.gene002590	MS.gene22730	PPI
MS.gene40828	MS.gene22730	PPI
MS.gene43924	MS.gene22730	PPI
MS.gene046089	MS.gene22730	PPI
MS.gene047954	MS.gene22730	PPI
MS.gene35175	MS.gene22730	PPI
MS.gene036423	MS.gene22730	PPI
MS.gene22730	MS.gene012889	PPI
MS.gene22730	MS.gene34463	PPI
MS.gene054652	MS.gene22730	PPI
MS.gene013644	MS.gene22730	PPI
MS.gene22730	MS.gene61499	PPI
MS.gene72520	MS.gene22730	PPI
MS.gene66651	MS.gene22730	PPI
MS.gene96109	MS.gene22730	PPI
MS.gene35202	MS.gene22730	PPI
MS.gene051449	MS.gene22730	PPI
MS.gene63743	MS.gene22730	PPI
MS.gene48477	MS.gene22730	PPI
MS.gene045875	MS.gene22730	PPI
MS.gene30136	MS.gene22730	PPI
MS.gene22730	MS.gene025675	PPI
MS.gene008861	MS.gene22730	PPI
MS.gene61452	MS.gene22730	PPI
MS.gene73261	MS.gene22730	PPI
MS.gene024297	MS.gene22730	PPI
MS.gene83979	MS.gene22730	PPI
MS.gene016102	MS.gene22730	PPI
MS.gene46941	MS.gene22730	PPI
MS.gene96541	MS.gene22730	PPI
MS.gene000634	MS.gene22730	PPI
MS.gene85295	MS.gene22730	PPI
MS.gene47792	MS.gene22730	PPI
MS.gene46948	MS.gene22730	PPI
MS.gene98471	MS.gene22730	PPI
MS.gene22730	MS.gene022929	PPI
MS.gene22730	MS.gene007022	PPI
MS.gene012019	MS.gene22730	PPI
MS.gene22730	MS.gene69584	PPI
MS.gene22730	MS.gene52349	PPI
MS.gene93396	MS.gene22730	PPI
MS.gene074257	MS.gene22730	PPI
MS.gene05231	MS.gene22730	PPI
MS.gene22730	MS.gene048342	PPI
MS.gene22730	MS.gene069624	PPI
MS.gene010656	MS.gene22730	PPI
MS.gene000629	MS.gene22730	PPI
MS.gene059269	MS.gene22730	PPI
MS.gene40469	MS.gene22730	PPI
MS.gene041264	MS.gene22730	PPI
MS.gene41979	MS.gene22730	PPI
MS.gene024522	MS.gene22730	PPI
MS.gene05319	MS.gene22730	PPI
MS.gene22730	MS.gene016719	PPI
MS.gene22730	MS.gene028714	PPI
MS.gene56991	MS.gene22730	PPI
MS.gene22730	MS.gene88393	PPI
MS.gene050679	MS.gene22730	PPI
MS.gene22730	MS.gene048706	PPI
MS.gene22730	MS.gene69586	PPI
MS.gene85538	MS.gene22730	PPI
MS.gene22730	MS.gene45083	PPI
MS.gene22730	MS.gene049561	PPI
MS.gene20246	MS.gene22730	PPI
MS.gene055223	MS.gene22730	PPI
MS.gene22730	MS.gene008658	PPI
MS.gene053747	MS.gene22730	PPI
MS.gene22730	MS.gene63150	PPI
MS.gene65471	MS.gene22730	PPI
MS.gene061850	MS.gene22730	PPI
MS.gene22730	MS.gene08708	PPI
MS.gene22730	MS.gene06164	PPI
MS.gene073582	MS.gene22730	PPI
MS.gene058889	MS.gene22730	PPI
MS.gene98616	MS.gene22730	PPI
MS.gene22730	MS.gene08606	PPI
MS.gene22730	MS.gene000640	PPI
MS.gene042027	MS.gene22730	PPI
MS.gene22730	MS.gene000506	PPI
MS.gene69490	MS.gene22730	PPI
MS.gene22730	MS.gene98756	PPI
MS.gene015248	MS.gene22730	PPI
MS.gene22730	MS.gene028716	PPI
MS.gene22730	MS.gene74639	PPI
MS.gene22730	MS.gene071133	PPI
MS.gene80362	MS.gene22730	PPI
MS.gene22730	MS.gene60266	PPI
MS.gene44083	MS.gene22730	PPI
MS.gene013193	MS.gene22730	PPI
MS.gene04970	MS.gene22730	PPI
MS.gene08106	MS.gene22730	PPI
MS.gene22730	MS.gene54051	PPI
MS.gene22730	MS.gene22111	PPI
MS.gene58419	MS.gene22730	PPI
MS.gene22730	MS.gene010290	PPI
MS.gene22730	MS.gene37955	PPI
MS.gene22730	MS.gene92517	PPI
MS.gene22730	MS.gene010890	PPI
MS.gene22730	MS.gene84790	PPI
MS.gene23521	MS.gene22730	PPI
MS.gene63150	MS.gene22730	PPI
MS.gene86275	MS.gene22730	PPI
MS.gene87529	MS.gene22730	PPI
MS.gene024405	MS.gene22730	PPI
MS.gene012748	MS.gene22730	PPI
MS.gene04134	MS.gene22730	PPI
MS.gene062067	MS.gene22730	PPI
MS.gene22730	MS.gene060788	PPI
MS.gene22730	MS.gene77990	PPI
MS.gene22730	MS.gene062511	PPI
MS.gene57044	MS.gene22730	PPI
MS.gene22730	MS.gene027994	PPI
MS.gene89815	MS.gene22730	PPI
MS.gene22730	MS.gene059269	PPI
MS.gene028714	MS.gene22730	PPI
MS.gene049610	MS.gene22730	PPI
MS.gene017586	MS.gene22730	PPI
MS.gene038676	MS.gene22730	PPI
MS.gene060718	MS.gene22730	PPI
MS.gene71637	MS.gene22730	PPI
MS.gene042651	MS.gene22730	PPI
MS.gene05576	MS.gene22730	PPI
MS.gene22730	MS.gene46353	PPI
MS.gene52349	MS.gene22730	PPI
MS.gene22730	MS.gene51799	PPI
MS.gene98467	MS.gene22730	PPI
MS.gene22730	MS.gene61651	PPI
MS.gene037049	MS.gene22730	PPI
MS.gene034643	MS.gene22730	PPI
MS.gene037634	MS.gene22730	PPI
MS.gene64130	MS.gene22730	PPI
MS.gene22730	MS.gene09023	PPI
MS.gene22730	MS.gene054508	PPI
MS.gene22730	MS.gene025987	PPI
MS.gene22730	MS.gene95960	PPI
MS.gene22730	MS.gene053373	PPI
MS.gene22730	MS.gene043140	PPI
MS.gene016719	MS.gene22730	PPI
MS.gene071784	MS.gene22730	PPI
MS.gene061579	MS.gene22730	PPI
MS.gene76420	MS.gene22730	PPI
MS.gene071320	MS.gene22730	PPI
MS.gene42903	MS.gene22730	PPI
MS.gene81712	MS.gene22730	PPI
MS.gene22730	MS.gene65474	PPI
MS.gene22730	MS.gene025401	PPI
MS.gene026680	MS.gene22730	PPI
MS.gene26433	MS.gene22730	PPI
MS.gene22730	MS.gene015093	PPI
MS.gene22730	MS.gene50392	PPI
MS.gene048747	MS.gene22730	PPI
MS.gene22730	MS.gene24278	PPI
MS.gene22730	MS.gene000379	PPI
MS.gene22730	MS.gene032299	PPI
MS.gene042559	MS.gene22730	PPI
MS.gene22200	MS.gene22730	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22730.t1	MTR_3g053920	67.797	295	68	4	1	283	1	280	1.10e-130	375
MS.gene22730.t1	MTR_2g061500	86.047	129	18	0	1	129	1	129	1.16e-78	237
MS.gene22730.t1	MTR_4g103530	61.307	199	56	6	55	250	1	181	6.89e-73	224
MS.gene22730.t1	MTR_8g463110	69.421	121	37	0	226	346	1	121	1.40e-38	145
MS.gene22730.t1	MTR_7g011710	69.412	85	26	0	55	139	1	85	5.11e-36	128
MS.gene22730.t1	MTR_0010s0370	37.430	179	98	6	8	177	4	177	5.17e-32	119
MS.gene22730.t1	MTR_0010s0350	37.430	179	98	6	8	177	4	177	5.17e-32	119
MS.gene22730.t1	MTR_6g033215	33.333	159	104	1	31	187	124	282	7.79e-29	113
MS.gene22730.t1	MTR_1g036720	32.039	103	70	0	11	113	9	111	9.88e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 107 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTGAATCACATTGATTTC+TGG	0.138202	7.4:-665669	MS.gene22730:CDS
TCGAGAACAGCAACGGTTTC+TGG	0.167681	7.4:+665940	None:intergenic
CATCGACCTTGTGGTGAAAT+TGG	0.244441	7.4:+665785	None:intergenic
AGACATACGCTCACTGAAAT+AGG	0.256666	7.4:+665870	None:intergenic
TGGCTCAGATCCGAGGTTTA+TGG	0.278490	7.4:+665960	None:intergenic
ACGACTATCATAAGACTTAA+AGG	0.281382	7.4:+665573	None:intergenic
GTCTGACGGTGAATTTGTGC+TGG	0.326563	7.4:-665002	MS.gene22730:CDS
TTTGTGCTGGGTTTGGCTGC+TGG	0.328076	7.4:-664989	MS.gene22730:CDS
GTGATTCAGTGGGACAAAAC+TGG	0.330797	7.4:+665681	None:intergenic
CGGTGAATTTGTGCTGGGTT+TGG	0.347218	7.4:-664996	MS.gene22730:CDS
GTTCTCGAACATTGCCTAAT+CGG	0.366703	7.4:-665925	MS.gene22730:CDS
ACTGAATCACATTGATTTCT+GGG	0.367380	7.4:-665668	MS.gene22730:CDS
GATGAGGGAAACATTGTAAT+AGG	0.368014	7.4:-664848	MS.gene22730:CDS
CTCTTACCTTCTTCCTCAAC+AGG	0.368499	7.4:-665046	MS.gene22730:CDS
CGTGGTACTGAAGCAGGAAT+AGG	0.371280	7.4:+665259	None:intergenic
ACCATCGATGTTAAGGTTGT+TGG	0.386606	7.4:+666008	None:intergenic
CAAGTCCACGGTTTACCCTT+TGG	0.387847	7.4:-665643	MS.gene22730:CDS
TACCTTGACCTACTTTCTGT+TGG	0.393464	7.4:+665614	None:intergenic
AGGTGCTAGTGGTTCTTGTC+AGG	0.398289	7.4:-665074	MS.gene22730:CDS
GTAATTGTAACTTTCTTGCC+TGG	0.398943	7.4:+665835	None:intergenic
CAGCAAGGACACGACCGATT+AGG	0.408317	7.4:+665911	None:intergenic
CCTTGTGGTGAAATTGGAAT+AGG	0.425107	7.4:+665791	None:intergenic
CCATCGATGTTAAGGTTGTT+GGG	0.431832	7.4:+666009	None:intergenic
GTTTAGCCTTTGCATCAACT+TGG	0.433761	7.4:+665104	None:intergenic
GTTAAGGTTGTTGGGATCCA+TGG	0.436099	7.4:+666017	None:intergenic
ACAAAACTGGGTACGACACC+AGG	0.437274	7.4:+665694	None:intergenic
AGGTCAGACCATCGATGTTA+AGG	0.439277	7.4:+666001	None:intergenic
CACGCCATTCTTGTTTCAAA+GGG	0.439548	7.4:+665497	None:intergenic
GCAAAGGCTAAACACGGTTC+AGG	0.442846	7.4:-665094	MS.gene22730:CDS
TAGTAACGGTTCCTTTGTTA+CGG	0.443190	7.4:-665467	MS.gene22730:intron
GCTGGTCCGTTTCTTAATGC+TGG	0.447601	7.4:-664971	MS.gene22730:CDS
ATCATTCTGTCGTTGAAACT+TGG	0.449924	7.4:+665175	None:intergenic
ATGTCTCGTGCTTGGAAGCC+AGG	0.453399	7.4:-665853	MS.gene22730:CDS
CAGAAAGTAGGTCAAGGTAT+AGG	0.453798	7.4:-665610	MS.gene22730:CDS
CTGGTTCCGATTTGAAGAAA+AGG	0.462538	7.4:-664925	MS.gene22730:CDS
GGTTAAACCCTCCTCTGATA+AGG	0.475559	7.4:+665981	None:intergenic
TTCTGTTGGATGAAACCAAA+GGG	0.480894	7.4:+665628	None:intergenic
GAATCATCATTTGCTGCTGA+TGG	0.486408	7.4:-664872	MS.gene22730:CDS
TCTGACGGTGAATTTGTGCT+GGG	0.491645	7.4:-665001	MS.gene22730:CDS
CTGTCCCTTTGAAACAAGAA+TGG	0.493273	7.4:-665501	MS.gene22730:CDS
GTGGTACTGAAGCAGGAATA+GGG	0.493715	7.4:+665260	None:intergenic
TTATAGCAGTTAATTGACTC+TGG	0.497479	7.4:+665146	None:intergenic
ACACGCCATTCTTGTTTCAA+AGG	0.498805	7.4:+665496	None:intergenic
CAGAAATCAATGTGATTCAG+TGG	0.501051	7.4:+665670	None:intergenic
AAGGATGCTGTTGTTCCTGC+TGG	0.502485	7.4:-665229	MS.gene22730:CDS
AATCATCATTTGCTGCTGAT+GGG	0.504180	7.4:-664871	MS.gene22730:CDS
AAATGCAACAGATGGTGGTC+AGG	0.508197	7.4:-665203	MS.gene22730:CDS
ACTTTCATATTGTCATGGAC+CGG	0.511440	7.4:-665723	MS.gene22730:CDS
ACCGTCAGACAGCAAGTTTG+AGG	0.513455	7.4:+665015	None:intergenic
GGTTTCATCCAACAGAAAGT+AGG	0.516836	7.4:-665622	MS.gene22730:CDS
ATGATTCTTCACCATTCTGA+AGG	0.517798	7.4:+664888	None:intergenic
TAGGTACAAATGTTAGTCAT+GGG	0.524354	7.4:-664829	MS.gene22730:CDS
CGCTGCCCAAGTTGATGCAA+AGG	0.524493	7.4:-665110	MS.gene22730:CDS
ATTTGCTGCTGATGGGGATG+AGG	0.526943	7.4:-664864	MS.gene22730:CDS
GAAGAAAAGGAAGAGAATGT+TGG	0.533755	7.4:-664912	MS.gene22730:CDS
GTGAGCGTATGTCTCGTGCT+TGG	0.540385	7.4:-665861	MS.gene22730:CDS
GCCTCAAACTTGCTGTCTGA+CGG	0.540508	7.4:-665016	MS.gene22730:CDS
CAAAGGGACAGTAACATCAA+TGG	0.541078	7.4:+665513	None:intergenic
GATTTCTGGGTGCAAGTCCA+CGG	0.545064	7.4:-665655	MS.gene22730:CDS
CGGTTTCTGGCTCAGATCCG+AGG	0.545071	7.4:+665953	None:intergenic
ATAGGTACAAATGTTAGTCA+TGG	0.550331	7.4:-664830	MS.gene22730:CDS
TGATAACTTTCATATTGTCA+TGG	0.552037	7.4:-665728	MS.gene22730:CDS
TGGCGTGTCCGTGCTAGTAA+CGG	0.557920	7.4:-665481	MS.gene22730:CDS
GCTGCACGCATCCTTGATGA+AGG	0.558147	7.4:-665763	MS.gene22730:CDS
GTGTGTCGTGGTACTGAAGC+AGG	0.559588	7.4:+665253	None:intergenic
GTACGACACCAGGGGCAATC+CGG	0.560950	7.4:+665704	None:intergenic
TTTAGCCTTTGCATCAACTT+GGG	0.561228	7.4:+665105	None:intergenic
GTCATGGACCGGATTGCCCC+TGG	0.563027	7.4:-665712	MS.gene22730:CDS
GAATTACCTGTTGAGGAAGA+AGG	0.564877	7.4:+665040	None:intergenic
TCTGTTGCATTTCGACCAGC+AGG	0.566046	7.4:+665214	None:intergenic
TGATTCAGTGGGACAAAACT+GGG	0.566580	7.4:+665682	None:intergenic
CCTATTCCAATTTCACCACA+AGG	0.568495	7.4:-665791	MS.gene22730:CDS
TCACAGCCAGCATTAAGAAA+CGG	0.571104	7.4:+664965	None:intergenic
CCCAACAACCTTAACATCGA+TGG	0.575183	7.4:-666009	MS.gene22730:CDS
TGAGGCTGAATTACCTGTTG+AGG	0.590417	7.4:+665033	None:intergenic
CAAAGGAACCGTTACTAGCA+CGG	0.590633	7.4:+665473	None:intergenic
AGAATGTTGGCCCTTCAGAA+TGG	0.594097	7.4:-664899	MS.gene22730:CDS
GAATACGCATATAAGAGCTA+TGG	0.602257	7.4:+665542	None:intergenic
TGGTCTGACCTTATCAGAGG+AGG	0.603900	7.4:-665989	MS.gene22730:CDS
GGTCTGACCTTATCAGAGGA+GGG	0.610448	7.4:-665988	MS.gene22730:CDS
ACAGCCACAAGCCTTCATCA+AGG	0.610772	7.4:+665752	None:intergenic
ATTGAATTTCGCGATCAGCA+AGG	0.610992	7.4:+665896	None:intergenic
ATCATCATTTGCTGCTGATG+GGG	0.612968	7.4:-664870	MS.gene22730:CDS
TTCAGTACCACGACACACAA+AGG	0.613295	7.4:-665248	MS.gene22730:CDS
GCATCCTTGATGAAGGCTTG+TGG	0.613393	7.4:-665756	MS.gene22730:CDS
TTTGCTGCTGATGGGGATGA+GGG	0.615655	7.4:-664863	MS.gene22730:CDS
TTTAAAAGTAACCGTAACAA+AGG	0.615879	7.4:+665456	None:intergenic
ATCCAACAGAAAGTAGGTCA+AGG	0.616872	7.4:-665616	MS.gene22730:CDS
TGATTCTTCACCATTCTGAA+GGG	0.621952	7.4:+664889	None:intergenic
GCAATGTTCGAGAACAGCAA+CGG	0.623043	7.4:+665933	None:intergenic
AGGTACAAATGTTAGTCATG+GGG	0.624100	7.4:-664828	MS.gene22730:CDS
GGTACAAATGTTAGTCATGG+GGG	0.629053	7.4:-664827	MS.gene22730:CDS
AAACACGGTTCAGGTGCTAG+TGG	0.636490	7.4:-665085	MS.gene22730:CDS
CAAAACTGGGTACGACACCA+GGG	0.636645	7.4:+665695	None:intergenic
CGATGGTCTGACCTTATCAG+AGG	0.640340	7.4:-665992	MS.gene22730:CDS
AGAAATCAATGTGATTCAGT+GGG	0.641223	7.4:+665671	None:intergenic
TTTCTGTTGGATGAAACCAA+AGG	0.643348	7.4:+665627	None:intergenic
ACAGCATCCTTTGTGTGTCG+TGG	0.648542	7.4:+665241	None:intergenic
GTTTCTTAATGCTGGCTGTG+AGG	0.660341	7.4:-664963	MS.gene22730:CDS
GCTGGTCGAAATGCAACAGA+TGG	0.671483	7.4:-665211	MS.gene22730:CDS
GGTCGAAATGCAACAGATGG+TGG	0.674246	7.4:-665208	MS.gene22730:CDS
GTACAAATGTTAGTCATGGG+GGG	0.677618	7.4:-664826	MS.gene22730:CDS
GAGGGTTTAACCATAAACCT+CGG	0.695563	7.4:-665970	MS.gene22730:CDS
AAAACTGGGTACGACACCAG+GGG	0.705794	7.4:+665696	None:intergenic
GTTGATGCAAAGGCTAAACA+CGG	0.707247	7.4:-665100	MS.gene22730:CDS
TGCGTGCAGCATCGACCTTG+TGG	0.712710	7.4:+665776	None:intergenic
TGAAACCAAAGGGTAAACCG+TGG	0.718993	7.4:+665638	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTTTTAAATATGAAAAGCT+GGG	-	chr7.4:665387-665406	MS.gene22730:intron	20.0%
!!!	TACTTTTAAATATGAAAAGC+TGG	-	chr7.4:665386-665405	MS.gene22730:intron	20.0%
!	TGATAACTTTCATATTGTCA+TGG	-	chr7.4:665101-665120	MS.gene22730:CDS	25.0%
!	TTTAAAAGTAACCGTAACAA+AGG	+	chr7.4:665376-665395	None:intergenic	25.0%
	ACGACTATCATAAGACTTAA+AGG	+	chr7.4:665259-665278	None:intergenic	30.0%
	AGAAATCAATGTGATTCAGT+GGG	+	chr7.4:665161-665180	None:intergenic	30.0%
	ATAGGTACAAATGTTAGTCA+TGG	-	chr7.4:665999-666018	MS.gene22730:CDS	30.0%
	TAGGTACAAATGTTAGTCAT+GGG	-	chr7.4:666000-666019	MS.gene22730:CDS	30.0%
!	TCTCTTCCTTTTCTTCAAAT+CGG	+	chr7.4:665913-665932	None:intergenic	30.0%
!	TTATAGCAGTTAATTGACTC+TGG	+	chr7.4:665686-665705	None:intergenic	30.0%
!!	ACTGAATCACATTGATTTCT+GGG	-	chr7.4:665161-665180	MS.gene22730:CDS	30.0%
	ACTTTCATATTGTCATGGAC+CGG	-	chr7.4:665106-665125	MS.gene22730:CDS	35.0%
	AGGTACAAATGTTAGTCATG+GGG	-	chr7.4:666001-666020	MS.gene22730:CDS	35.0%
	ATCATTCTGTCGTTGAAACT+TGG	+	chr7.4:665657-665676	None:intergenic	35.0%
	ATGATTCTTCACCATTCTGA+AGG	+	chr7.4:665944-665963	None:intergenic	35.0%
	ATTCCAGCTCAAACAATTCT+GGG	+	chr7.4:665461-665480	None:intergenic	35.0%
	CAGAAATCAATGTGATTCAG+TGG	+	chr7.4:665162-665181	None:intergenic	35.0%
	GAAGAAAAGGAAGAGAATGT+TGG	-	chr7.4:665917-665936	MS.gene22730:CDS	35.0%
	GAATACGCATATAAGAGCTA+TGG	+	chr7.4:665290-665309	None:intergenic	35.0%
	GATGAGGGAAACATTGTAAT+AGG	-	chr7.4:665981-666000	MS.gene22730:CDS	35.0%
	GTAATTGTAACTTTCTTGCC+TGG	+	chr7.4:664997-665016	None:intergenic	35.0%
	TAGTAACGGTTCCTTTGTTA+CGG	-	chr7.4:665362-665381	MS.gene22730:intron	35.0%
	TGATTCTTCACCATTCTGAA+GGG	+	chr7.4:665943-665962	None:intergenic	35.0%
	TTCTGTTGGATGAAACCAAA+GGG	+	chr7.4:665204-665223	None:intergenic	35.0%
	TTTCTGTTGGATGAAACCAA+AGG	+	chr7.4:665205-665224	None:intergenic	35.0%
!	AATCATCATTTGCTGCTGAT+GGG	-	chr7.4:665958-665977	MS.gene22730:CDS	35.0%
!	CAAGGTATAGGTAGCTTTTT+AGG	-	chr7.4:665231-665250	MS.gene22730:CDS	35.0%
!	TTTAGCCTTTGCATCAACTT+GGG	+	chr7.4:665727-665746	None:intergenic	35.0%
!!	CACTGAATCACATTGATTTC+TGG	-	chr7.4:665160-665179	MS.gene22730:CDS	35.0%
	AAGCCAGTTACTCAAAGGAT+AGG	-	chr7.4:665516-665535	MS.gene22730:CDS	40.0%
	ACCATCGATGTTAAGGTTGT+TGG	+	chr7.4:664824-664843	None:intergenic	40.0%
	ACCTTAAGCCAGTTACTCAA+AGG	-	chr7.4:665511-665530	MS.gene22730:CDS	40.0%
	AGACATACGCTCACTGAAAT+AGG	+	chr7.4:664962-664981	None:intergenic	40.0%
	ATCCAACAGAAAGTAGGTCA+AGG	-	chr7.4:665213-665232	MS.gene22730:CDS	40.0%
	ATTGAATTTCGCGATCAGCA+AGG	+	chr7.4:664936-664955	None:intergenic	40.0%
	CAAAGGGACAGTAACATCAA+TGG	+	chr7.4:665319-665338	None:intergenic	40.0%
	CAGAAAGTAGGTCAAGGTAT+AGG	-	chr7.4:665219-665238	MS.gene22730:CDS	40.0%
	CCATCGATGTTAAGGTTGTT+GGG	+	chr7.4:664823-664842	None:intergenic	40.0%
	CCTATTCCAATTTCACCACA+AGG	-	chr7.4:665038-665057	MS.gene22730:CDS	40.0%
	CCTTGTGGTGAAATTGGAAT+AGG	+	chr7.4:665041-665060	None:intergenic	40.0%
	GAACCTATCCTTTGAGTAAC+TGG	+	chr7.4:665522-665541	None:intergenic	40.0%
	GAATTACCTGTTGAGGAAGA+AGG	+	chr7.4:665792-665811	None:intergenic	40.0%
	GAGGGTTTAACCATAAACCT+CGG	-	chr7.4:664859-664878	MS.gene22730:CDS	40.0%
	GATTCCAGCTCAAACAATTC+TGG	+	chr7.4:665462-665481	None:intergenic	40.0%
	GGTACAAATGTTAGTCATGG+GGG	-	chr7.4:666002-666021	MS.gene22730:CDS	40.0%
	GGTTTCATCCAACAGAAAGT+AGG	-	chr7.4:665207-665226	MS.gene22730:CDS	40.0%
	GTACAAATGTTAGTCATGGG+GGG	-	chr7.4:666003-666022	MS.gene22730:CDS	40.0%
	GTTCTCGAACATTGCCTAAT+CGG	-	chr7.4:664904-664923	MS.gene22730:CDS	40.0%
	GTTGATGCAAAGGCTAAACA+CGG	-	chr7.4:665729-665748	MS.gene22730:CDS	40.0%
	TACCTTGACCTACTTTCTGT+TGG	+	chr7.4:665218-665237	None:intergenic	40.0%
	TCACAGCCAGCATTAAGAAA+CGG	+	chr7.4:665867-665886	None:intergenic	40.0%
	TGATTCAGTGGGACAAAACT+GGG	+	chr7.4:665150-665169	None:intergenic	40.0%
	TTTGAGCTGGAATCTGATGA+CGG	-	chr7.4:665468-665487	MS.gene22730:CDS	40.0%
!	ACACGCCATTCTTGTTTCAA+AGG	+	chr7.4:665336-665355	None:intergenic	40.0%
!	ATCATCATTTGCTGCTGATG+GGG	-	chr7.4:665959-665978	MS.gene22730:CDS	40.0%
!	CACGCCATTCTTGTTTCAAA+GGG	+	chr7.4:665335-665354	None:intergenic	40.0%
!	CTGGTTCCGATTTGAAGAAA+AGG	-	chr7.4:665904-665923	MS.gene22730:CDS	40.0%
!	GAATCATCATTTGCTGCTGA+TGG	-	chr7.4:665957-665976	MS.gene22730:CDS	40.0%
!	GAGGAGTCTCATGATTTTAC+TGG	-	chr7.4:665885-665904	MS.gene22730:CDS	40.0%
!	GATGACGGTGTTCGTAATTA+GGG	-	chr7.4:665483-665502	MS.gene22730:CDS	40.0%
!	GTTTAGCCTTTGCATCAACT+TGG	+	chr7.4:665728-665747	None:intergenic	40.0%
!	TCCTTTGAGTAACTGGCTTA+AGG	+	chr7.4:665515-665534	None:intergenic	40.0%
!	TTGAGTAACTGGCTTAAGGT+AGG	+	chr7.4:665511-665530	None:intergenic	40.0%
!!	CTGTCCCTTTGAAACAAGAA+TGG	-	chr7.4:665328-665347	MS.gene22730:intron	40.0%
!!	TGATGACGGTGTTCGTAATT+AGG	-	chr7.4:665482-665501	MS.gene22730:CDS	40.0%
	AGAATGTTGGCCCTTCAGAA+TGG	-	chr7.4:665930-665949	MS.gene22730:CDS	45.0%
	AGGTCAGACCATCGATGTTA+AGG	+	chr7.4:664831-664850	None:intergenic	45.0%
	CAAAGGAACCGTTACTAGCA+CGG	+	chr7.4:665359-665378	None:intergenic	45.0%
	CATCGACCTTGTGGTGAAAT+TGG	+	chr7.4:665047-665066	None:intergenic	45.0%
	CCCAACAACCTTAACATCGA+TGG	-	chr7.4:664820-664839	MS.gene22730:CDS	45.0%
	CTCTTACCTTCTTCCTCAAC+AGG	-	chr7.4:665783-665802	MS.gene22730:CDS	45.0%
	GCAATGTTCGAGAACAGCAA+CGG	+	chr7.4:664899-664918	None:intergenic	45.0%
	GGTTAAACCCTCCTCTGATA+AGG	+	chr7.4:664851-664870	None:intergenic	45.0%
	GTGATTCAGTGGGACAAAAC+TGG	+	chr7.4:665151-665170	None:intergenic	45.0%
	TCTGACGGTGAATTTGTGCT+GGG	-	chr7.4:665828-665847	MS.gene22730:CDS	45.0%
	TGAAACCAAAGGGTAAACCG+TGG	+	chr7.4:665194-665213	None:intergenic	45.0%
	TGAGGCTGAATTACCTGTTG+AGG	+	chr7.4:665799-665818	None:intergenic	45.0%
	TTCAGTACCACGACACACAA+AGG	-	chr7.4:665581-665600	MS.gene22730:CDS	45.0%
!	AAATGCAACAGATGGTGGTC+AGG	-	chr7.4:665626-665645	MS.gene22730:CDS	45.0%
!	GTGGTACTGAAGCAGGAATA+GGG	+	chr7.4:665572-665591	None:intergenic	45.0%
!	GTTTCTTAATGCTGGCTGTG+AGG	-	chr7.4:665866-665885	MS.gene22730:CDS	45.0%
!!!	AGCTGGGTGTTTTTTGTCAC+AGG	-	chr7.4:665403-665422	MS.gene22730:intron	45.0%
!!!	GGTGTTTTTTGTCACAGGTG+CGG	-	chr7.4:665408-665427	MS.gene22730:intron	45.0%
	AAAACTGGGTACGACACCAG+GGG	+	chr7.4:665136-665155	None:intergenic	50.0%
	AAACACGGTTCAGGTGCTAG+TGG	-	chr7.4:665744-665763	MS.gene22730:CDS	50.0%
	AAGGATGCTGTTGTTCCTGC+TGG	-	chr7.4:665600-665619	MS.gene22730:CDS	50.0%
	ACAAAACTGGGTACGACACC+AGG	+	chr7.4:665138-665157	None:intergenic	50.0%
	ACAGCATCCTTTGTGTGTCG+TGG	+	chr7.4:665591-665610	None:intergenic	50.0%
	ACAGCCACAAGCCTTCATCA+AGG	+	chr7.4:665080-665099	None:intergenic	50.0%
	ACCGTCAGACAGCAAGTTTG+AGG	+	chr7.4:665817-665836	None:intergenic	50.0%
	AGGTGCTAGTGGTTCTTGTC+AGG	-	chr7.4:665755-665774	MS.gene22730:CDS	50.0%
	CAAAACTGGGTACGACACCA+GGG	+	chr7.4:665137-665156	None:intergenic	50.0%
	CAAGTCCACGGTTTACCCTT+TGG	-	chr7.4:665186-665205	MS.gene22730:CDS	50.0%
	CGATGGTCTGACCTTATCAG+AGG	-	chr7.4:664837-664856	MS.gene22730:CDS	50.0%
	CGGTGAATTTGTGCTGGGTT+TGG	-	chr7.4:665833-665852	MS.gene22730:CDS	50.0%
	GCAAAGGCTAAACACGGTTC+AGG	-	chr7.4:665735-665754	MS.gene22730:CDS	50.0%
	GCATCCTTGATGAAGGCTTG+TGG	-	chr7.4:665073-665092	MS.gene22730:CDS	50.0%
	GCTGGTCCGTTTCTTAATGC+TGG	-	chr7.4:665858-665877	MS.gene22730:CDS	50.0%
	GCTGGTCGAAATGCAACAGA+TGG	-	chr7.4:665618-665637	MS.gene22730:CDS	50.0%
	GGTCGAAATGCAACAGATGG+TGG	-	chr7.4:665621-665640	MS.gene22730:CDS	50.0%
	GGTCTGACCTTATCAGAGGA+GGG	-	chr7.4:664841-664860	MS.gene22730:CDS	50.0%
	GTCTGACGGTGAATTTGTGC+TGG	-	chr7.4:665827-665846	MS.gene22730:CDS	50.0%
	GTGCCCAGAATTGTTTGAGC+TGG	-	chr7.4:665455-665474	MS.gene22730:intron	50.0%
	GTTCTGCTGCAACCAACTGA+TGG	-	chr7.4:665538-665557	MS.gene22730:CDS	50.0%
	TCGAGAACAGCAACGGTTTC+TGG	+	chr7.4:664892-664911	None:intergenic	50.0%
	TCTGTTGCATTTCGACCAGC+AGG	+	chr7.4:665618-665637	None:intergenic	50.0%
	TGCAACCAACTGATGGCTAC+AGG	-	chr7.4:665545-665564	MS.gene22730:CDS	50.0%
	TGGCTCAGATCCGAGGTTTA+TGG	+	chr7.4:664872-664891	None:intergenic	50.0%
	TGGTCTGACCTTATCAGAGG+AGG	-	chr7.4:664840-664859	MS.gene22730:CDS	50.0%
	TTTGCTGCTGATGGGGATGA+GGG	-	chr7.4:665966-665985	MS.gene22730:CDS	50.0%
!	ATTTGCTGCTGATGGGGATG+AGG	-	chr7.4:665965-665984	MS.gene22730:CDS	50.0%
!	CGTGGTACTGAAGCAGGAAT+AGG	+	chr7.4:665573-665592	None:intergenic	50.0%
!	GATTTCTGGGTGCAAGTCCA+CGG	-	chr7.4:665174-665193	MS.gene22730:CDS	50.0%
!!	GCCTCAAACTTGCTGTCTGA+CGG	-	chr7.4:665813-665832	MS.gene22730:CDS	50.0%
	AGGGTCCTGTAGCCATCAGT+TGG	+	chr7.4:665553-665572	None:intergenic	55.0%
	ATGTCTCGTGCTTGGAAGCC+AGG	-	chr7.4:664976-664995	MS.gene22730:CDS	55.0%
	CAGCAAGGACACGACCGATT+AGG	+	chr7.4:664921-664940	None:intergenic	55.0%
	GCTGCACGCATCCTTGATGA+AGG	-	chr7.4:665066-665085	MS.gene22730:CDS	55.0%
	GTGAGCGTATGTCTCGTGCT+TGG	-	chr7.4:664968-664987	MS.gene22730:CDS	55.0%
	TGGCGTGTCCGTGCTAGTAA+CGG	-	chr7.4:665348-665367	MS.gene22730:intron	55.0%
	TGTCACAGGTGCGGTCTGTT+GGG	-	chr7.4:665417-665436	MS.gene22730:intron	55.0%
	TTGTCACAGGTGCGGTCTGT+TGG	-	chr7.4:665416-665435	MS.gene22730:intron	55.0%
	TTTGTGCTGGGTTTGGCTGC+TGG	-	chr7.4:665840-665859	MS.gene22730:CDS	55.0%
!	GTGTGTCGTGGTACTGAAGC+AGG	+	chr7.4:665579-665598	None:intergenic	55.0%
!!	CGCTGCCCAAGTTGATGCAA+AGG	-	chr7.4:665719-665738	MS.gene22730:CDS	55.0%
	GTACGACACCAGGGGCAATC+CGG	+	chr7.4:665128-665147	None:intergenic	60.0%
!!	CGGTTTCTGGCTCAGATCCG+AGG	+	chr7.4:664879-664898	None:intergenic	60.0%
!!	TGCGTGCAGCATCGACCTTG+TGG	+	chr7.4:665056-665075	None:intergenic	60.0%
	GTCATGGACCGGATTGCCCC+TGG	-	chr7.4:665117-665136	MS.gene22730:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	664814	666037	664814	ID=MS.gene22730
chr7.4	mRNA	664814	666037	664814	ID=MS.gene22730.t1;Parent=MS.gene22730
chr7.4	exon	665468	666037	665468	ID=MS.gene22730.t1.exon1;Parent=MS.gene22730.t1
chr7.4	CDS	665468	666037	665468	ID=cds.MS.gene22730.t1;Parent=MS.gene22730.t1
chr7.4	exon	664814	665284	664814	ID=MS.gene22730.t1.exon2;Parent=MS.gene22730.t1
chr7.4	CDS	664814	665284	664814	ID=cds.MS.gene22730.t1;Parent=MS.gene22730.t1

Gene Sequence

>MS.gene22730

ATGGATCCCAACAACCTTAACATCGATGGTCTGACCTTATCAGAGGAGGGTTTAACCATAAACCTCGGATCTGAGCCAGAAACCGTTGCTGTTCTCGAACATTGCCTAATCGGTCGTGTCCTTGCTGATCGCGAAATTCAATTCGCCTATTTCAGTGAGCGTATGTCTCGTGCTTGGAAGCCAGGCAAGAAAGTTACAATTACTAAGTCTGTGTCTGATCGTTACCTATTCCAATTTCACCACAAGGTCGATGCTGCACGCATCCTTGATGAAGGCTTGTGGCTGTATGATAACTTTCATATTGTCATGGACCGGATTGCCCCTGGTGTCGTACCCAGTTTTGTCCCACTGAATCACATTGATTTCTGGGTGCAAGTCCACGGTTTACCCTTTGGTTTCATCCAACAGAAAGTAGGTCAAGGTATAGGTAGCTTTTTAGGTACCTTTAAGTCTTATGATAGTCGTAACTCAATCCATAGCTCTTATATGCGTATTCGTGTTGCCATTGATGTTACTGTCCCTTTGAAACAAGAATGGCGTGTCCGTGCTAGTAACGGTTCCTTTGTTACGGTTACTTTTAAATATGAAAAGCTGGGTGTTTTTTGTCACAGGTGCGGTCTGTTGGGACATACTGACAAAGTGTGCCCAGAATTGTTTGAGCTGGAATCTGATGACGGTGTTCGTAATTAGGGCGCCTACCTTAAGCCAGTTACTCAAAGGATAGGTTCTGCTGCAACCAACTGATGGCTACAGGACCCTATTCCTGCTTCAGTACCACGACACACAAAGGATGCTGTTGTTCCTGCTGGTCGAAATGCAACAGATGGTGGTCAGGTTGTACCAAGTTTCAACGACAGAATGATTGCTGTCCAGAGTCAATTAACTGCTATAAAACATGATGTTCTCGCTGCCCAAGTTGATGCAAAGGCTAAACACGGTTCAGGTGCTAGTGGTTCTTGTCAGGTGCATCTCTTACCTTCTTCCTCAACAGGTAATTCAGCCTCAAACTTGCTGTCTGACGGTGAATTTGTGCTGGGTTTGGCTGCTGGTCCGTTTCTTAATGCTGGCTGTGAGGAGTCTCATGATTTTACTGGTTCCGATTTGAAGAAAAGGAAGAGAATGTTGGCCCTTCAGAATGGTGAAGAATCATCATTTGCTGCTGATGGGGATGAGGGAAACATTGTAATAGGTACAAATGTTAGTCATGGGGGGATGTTGAAATGA

Protein sequence

>MS.gene22730.t1

MDPNNLNIDGLTLSEEGLTINLGSEPETVAVLEHCLIGRVLADREIQFAYFSERMSRAWKPGKKVTITKSVSDRYLFQFHHKVDAARILDEGLWLYDNFHIVMDRIAPGVVPSFVPLNHIDFWVQVHGLPFGFIQQKVGQGIGSFLGTFKSYDSRNSIHSSYMRIRVAIDVTVPLKQEWRVRASNGSFVTDPIPASVPRHTKDAVVPAGRNATDGGQVVPSFNDRMIAVQSQLTAIKHDVLAAQVDAKAKHGSGASGSCQVHLLPSSSTGNSASNLLSDGEFVLGLAAGPFLNAGCEESHDFTGSDLKKRKRMLALQNGEESSFAADGDEGNIVIGTNVSHGGMLK