AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92517.t1 MTR_1g051330 87.121 132 17 0 148 279 20 151 3.35e-66 223
MS.gene92517.t1 MTR_1g051330 94.340 106 6 0 275 380 321 426 6.80e-64 217
MS.gene92517.t1 MTR_0010s0370 73.171 123 33 0 1 123 1 123 7.24e-60 192
MS.gene92517.t1 MTR_0010s0350 73.171 123 33 0 1 123 1 123 7.24e-60 192
MS.gene92517.t1 MTR_1g036720 69.369 111 34 0 1 111 1 111 2.62e-50 164
MS.gene92517.t1 MTR_6g046587 84.375 64 10 0 280 343 221 284 1.77e-22 96.3
MS.gene92517.t1 MTR_8g463110 42.157 102 58 1 280 380 171 272 1.02e-19 91.3
MS.gene92517.t1 MTR_1g100687 38.182 110 65 2 274 380 101 210 4.55e-17 80.1
MS.gene92517.t1 MTR_0042s0130 41.837 98 52 2 280 374 210 305 3.09e-16 79.3
MS.gene92517.t1 MTR_1g036710 33.129 163 75 5 128 266 63 215 1.29e-15 77.0
MS.gene92517.t1 MTR_6g009010 44.048 84 46 1 280 362 178 261 5.15e-15 74.7
MS.gene92517.t1 MTR_2g061500 35.849 106 66 1 34 139 36 139 3.10e-14 69.7
MS.gene92517.t1 MTR_3g053920 27.128 188 88 3 33 171 35 222 3.23e-13 69.7
MS.gene92517.t1 MTR_4g020630 37.500 104 52 3 275 375 110 203 3.36e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 103 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCAATTTGTTCCATTT+TGG 0.107646 4.2:-81485315 MS.gene92517:CDS
GGCTGCAGAGTTGGCAGTTT+TGG 0.187985 4.2:+81484826 None:intergenic
TGAATCTGCTGTCAGAAATT+TGG 0.251165 4.2:+81483562 None:intergenic
TTCAACCCTCTGGACTGTTT+TGG 0.253806 4.2:+81484677 None:intergenic
GTGGGAGGGTTTCATCAATT+TGG 0.263079 4.2:+81485602 None:intergenic
AAGCTTAACAATGGGGAAAT+TGG 0.264536 4.2:-81484761 MS.gene92517:CDS
ATTGAGTTTGTTGATAATAT+TGG 0.280224 4.2:+81483627 None:intergenic
GACCCTCGCCTCTGCCTTGT+TGG 0.296425 4.2:-81485571 MS.gene92517:CDS
AATCAAGATGATGTGCAAAT+TGG 0.314096 4.2:+81485431 None:intergenic
AGGATGATGGCACACGACTA+TGG 0.315161 4.2:-81484988 MS.gene92517:CDS
TCATGTACCTGTATCCAAAA+TGG 0.340379 4.2:+81485301 None:intergenic
ATCAATTCGTGAAGAGCAAC+TGG 0.347808 4.2:-81483666 MS.gene92517:CDS
AATTGAAGAGCAACTTGGTT+TGG 0.361325 4.2:+81485328 None:intergenic
GCGCTTTGGTCACATCTTCC+TGG 0.366376 4.2:-81483840 MS.gene92517:CDS
TTCACGAATTGATTGAGTTC+CGG 0.367261 4.2:+81483676 None:intergenic
TCCCCATTGTTAAGCTTTGA+TGG 0.368619 4.2:+81484767 None:intergenic
AATCAATGAGGAGCTGTTAC+AGG 0.371949 4.2:-81484732 MS.gene92517:CDS
TGGAAACAATAGAGACGTTC+TGG 0.374248 4.2:-81485507 MS.gene92517:CDS
TGGTTTGGAGCAGTATTCTC+TGG 0.376400 4.2:+81485343 None:intergenic
ACTGCTCGACCGTTACTGTT+AGG 0.381596 4.2:+81484860 None:intergenic
ATAAGCCTGGTTCTATCTCT+TGG 0.396666 4.2:+81485461 None:intergenic
ATCATCATATATGAACGAAT+TGG 0.403084 4.2:+81485529 None:intergenic
AAGGTCTGGAGGGCAGCGTT+TGG 0.403819 4.2:+81483805 None:intergenic
CATCGCTGAGAGAAAGTTGA+TGG 0.407274 4.2:+81485644 None:intergenic
AATATTGGACGGAGGGTAAT+GGG 0.410504 4.2:+81483642 None:intergenic
TTGGCTTATTTGTGTGGAAT+CGG 0.416297 4.2:+81485548 None:intergenic
ATATAGAGGCCACCAAGGTC+TGG 0.430899 4.2:+81483791 None:intergenic
AGGTCTGGAGGGCAGCGTTT+GGG 0.441661 4.2:+81483806 None:intergenic
GCCCATCAAAGCTTAACAAT+GGG 0.450446 4.2:-81484769 MS.gene92517:CDS
TAGGGTTGTCTCCAAGGTAT+AGG 0.451982 4.2:+81483736 None:intergenic
AGCCCGTATTGATCGCGCTT+TGG 0.452249 4.2:-81483854 MS.gene92517:CDS
TAATATTGGACGGAGGGTAA+TGG 0.453093 4.2:+81483641 None:intergenic
GGCGAGGGTCATCTGTTTGT+GGG 0.461045 4.2:+81485584 None:intergenic
GCTAGAGCATTAGTTTCTGT+TGG 0.466428 4.2:+81484584 None:intergenic
CCCCATTGTTAAGCTTTGAT+GGG 0.470780 4.2:+81484768 None:intergenic
AGGGTCATCTGTTTGTGGGA+GGG 0.474436 4.2:+81485588 None:intergenic
ATTTGCTAGGGCTGCAGAGT+TGG 0.483464 4.2:+81484817 None:intergenic
GGTTGCGCAATAAATTTGCT+AGG 0.487055 4.2:+81484804 None:intergenic
TGACCAAAGCGCGATCAATA+CGG 0.488446 4.2:+81483851 None:intergenic
GGTTTGGAGCAGTATTCTCT+GGG 0.493523 4.2:+81485344 None:intergenic
AGGAGTGTGATCAGATATAG+AGG 0.494114 4.2:+81483777 None:intergenic
CAGATATTTCATCTTGGAGC+AGG 0.494834 4.2:-81483594 MS.gene92517:CDS
CGCTGCCCTCCAGACCTTGG+TGG 0.506020 4.2:-81483800 MS.gene92517:CDS
ATTGACCTCATTCAACCCTC+TGG 0.506778 4.2:+81484667 None:intergenic
TTTCAGAGAATTCTAAAGAA+GGG 0.509065 4.2:-81485409 MS.gene92517:CDS
ATTTCTGTCTCATTAACTAC+AGG 0.510910 4.2:+81484611 None:intergenic
GGATGATGGCACACGACTAT+GGG 0.511194 4.2:-81484987 MS.gene92517:CDS
GACCAAAGCGCGATCAATAC+GGG 0.512179 4.2:+81483852 None:intergenic
AGGCGAGGGTCATCTGTTTG+TGG 0.513741 4.2:+81485583 None:intergenic
CGTCGTCAGACAGAATCTGC+GGG 0.518904 4.2:-81484914 MS.gene92517:CDS
AATTCGTTCATATATGATGA+TGG 0.525605 4.2:-81485527 MS.gene92517:CDS
GTGCGCAGGACTATTATCAT+TGG 0.528554 4.2:+81484544 None:intergenic
TTGCGGTAGGGTTGTCTCCA+AGG 0.529503 4.2:+81483730 None:intergenic
GAACAAATTGAAGAGCAACT+TGG 0.532733 4.2:+81485323 None:intergenic
GGCCAACAAGGCAGAGGCGA+GGG 0.539058 4.2:+81485569 None:intergenic
AAACGCTGCCCTCCAGACCT+TGG 0.539097 4.2:-81483803 MS.gene92517:CDS
TGCAGCTTGATCCTATACCT+TGG 0.541954 4.2:-81483747 MS.gene92517:CDS
GTTTGGGTATAGTGATTGCC+AGG 0.542128 4.2:+81483822 None:intergenic
CGCCCATCAAAGCTTAACAA+TGG 0.543839 4.2:-81484770 MS.gene92517:CDS
CTGGGATCACATTAAGAACC+AGG 0.557735 4.2:+81485362 None:intergenic
AACGAATTGGCTTATTTGTG+TGG 0.560361 4.2:+81485542 None:intergenic
GTGTCGCAGATATTTCATCT+TGG 0.560927 4.2:-81483600 MS.gene92517:CDS
GGATCAAGCTGCAGAAGCAG+AGG 0.560982 4.2:+81483757 None:intergenic
AATTGGAAAGTGAATAAGCC+TGG 0.567233 4.2:+81485448 None:intergenic
TGTTGATAATATTGGACGGA+GGG 0.568650 4.2:+81483635 None:intergenic
AAAATCCAAGAGATAGAACC+AGG 0.571721 4.2:-81485466 MS.gene92517:CDS
GTTGCGCAATAAATTTGCTA+GGG 0.573743 4.2:+81484805 None:intergenic
TATTCGGTTGATGAGGATGA+TGG 0.578464 4.2:-81485001 MS.gene92517:intron
CGGCCAACAAGGCAGAGGCG+AGG 0.581617 4.2:+81485568 None:intergenic
GGAATCGGCCAACAAGGCAG+AGG 0.586393 4.2:+81485563 None:intergenic
TTGTGTGGAATCGGCCAACA+AGG 0.586625 4.2:+81485557 None:intergenic
GAGGGTCATCTGTTTGTGGG+AGG 0.589063 4.2:+81485587 None:intergenic
GTGGTACTGACATGGTGCGC+AGG 0.591030 4.2:+81484530 None:intergenic
TTGATGGGCGCATTAAGTGC+GGG 0.591083 4.2:+81484783 None:intergenic
GAATTCTAAAGAAGGGTCCA+TGG 0.595524 4.2:-81485402 MS.gene92517:CDS
TTTGATGGGCGCATTAAGTG+CGG 0.598668 4.2:+81484782 None:intergenic
AACAAGCCAAAACAGTCCAG+AGG 0.599696 4.2:-81484683 MS.gene92517:CDS
TAGAGGCCACCAAGGTCTGG+AGG 0.603957 4.2:+81483794 None:intergenic
TCAAACCGAAAATTGCGGTA+GGG 0.608619 4.2:+81483718 None:intergenic
AGAGGCCACCAAGGTCTGGA+GGG 0.609616 4.2:+81483795 None:intergenic
ACGTTCTGGAATCCTGTCAA+AGG 0.611044 4.2:-81485493 MS.gene92517:CDS
GTCAAACCGAAAATTGCGGT+AGG 0.615655 4.2:+81483717 None:intergenic
TTGTTGATAATATTGGACGG+AGG 0.618733 4.2:+81483634 None:intergenic
AGTTTGTTGATAATATTGGA+CGG 0.622393 4.2:+81483631 None:intergenic
AGCTGCGTGTAGAAAAGCAA+CGG 0.623220 4.2:-81484958 MS.gene92517:CDS
TCACATTAAGAACCAGGAGG+TGG 0.623220 4.2:+81485368 None:intergenic
ACAGTCCAGAGGGTTGAATG+AGG 0.627491 4.2:-81484672 MS.gene92517:CDS
ATCAGATATAGAGGCCACCA+AGG 0.635869 4.2:+81483786 None:intergenic
ACAAGCCAAAACAGTCCAGA+GGG 0.644222 4.2:-81484682 MS.gene92517:CDS
GATGATGGCACACGACTATG+GGG 0.645736 4.2:-81484986 MS.gene92517:CDS
GCGTCGTCAGACAGAATCTG+CGG 0.646561 4.2:-81484915 MS.gene92517:CDS
TCGTCAGACAGAATCTGCGG+GGG 0.648613 4.2:-81484912 MS.gene92517:CDS
TCAACTTTCTCTCAGCGATG+AGG 0.652718 4.2:-81485641 MS.gene92517:CDS
TCAATTCGTGAAGAGCAACT+GGG 0.662431 4.2:-81483665 MS.gene92517:CDS
AGGTGGTTGTCAAAATACCA+TGG 0.664762 4.2:+81485385 None:intergenic
GTTACAGTTCCTAACAGTAA+CGG 0.665389 4.2:-81484869 MS.gene92517:CDS
AATTGTCAAACCGAAAATTG+CGG 0.668089 4.2:+81483713 None:intergenic
AATTGGTAACTCAATCAATG+AGG 0.679514 4.2:-81484744 MS.gene92517:CDS
GTCGTCAGACAGAATCTGCG+GGG 0.683390 4.2:-81484913 MS.gene92517:CDS
CCCATCAAAGCTTAACAATG+GGG 0.687788 4.2:-81484768 MS.gene92517:CDS
CGTCAGACAGAATCTGCGGG+GGG 0.698928 4.2:-81484911 MS.gene92517:CDS
ATGATGGCACACGACTATGG+GGG 0.726460 4.2:-81484985 MS.gene92517:CDS
GGATCACATTAAGAACCAGG+AGG 0.765577 4.2:+81485365 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATCAAAGATTCTGTTAT+TGG - chr4.2:81485066-81485085 MS.gene92517:intron 20.0%
!!! ATTGAGTTTGTTGATAATAT+TGG + chr4.2:81485604-81485623 None:intergenic 20.0%
! AAGACATGAATATAATATCG+TGG - chr4.2:81484791-81484810 MS.gene92517:CDS 25.0%
! AATTCGTTCATATATGATGA+TGG - chr4.2:81483701-81483720 MS.gene92517:CDS 25.0%
! ATCATCATATATGAACGAAT+TGG + chr4.2:81483702-81483721 None:intergenic 25.0%
! GATATTATATTCATGTCTTC+CGG + chr4.2:81484790-81484809 None:intergenic 25.0%
!! GAGATGATTGGAATTATTTT+TGG + chr4.2:81484030-81484049 None:intergenic 25.0%
!! TATCATTGGTAGATGATTTT+TGG + chr4.2:81484673-81484692 None:intergenic 25.0%
!! TTTCAGAGAATTCTAAAGAA+GGG - chr4.2:81483819-81483838 MS.gene92517:CDS 25.0%
!!! AGTTTGTTGATAATATTGGA+CGG + chr4.2:81485600-81485619 None:intergenic 25.0%
!!! TTTTCAGAGAATTCTAAAGA+AGG - chr4.2:81483818-81483837 MS.gene92517:CDS 25.0%
AAGAAAATCAAGAAACAAGG+AGG - chr4.2:81484107-81484126 MS.gene92517:intron 30.0%
AAGATTCTGTTATTGGTACA+TGG - chr4.2:81485073-81485092 MS.gene92517:intron 30.0%
AATCAAGATGATGTGCAAAT+TGG + chr4.2:81483800-81483819 None:intergenic 30.0%
AATTGTCAAACCGAAAATTG+CGG + chr4.2:81485518-81485537 None:intergenic 30.0%
ATTTCTGTCTCATTAACTAC+AGG + chr4.2:81484620-81484639 None:intergenic 30.0%
CAGAAGAAAATCAAGAAACA+AGG - chr4.2:81484104-81484123 MS.gene92517:intron 30.0%
TATAGTGGCTACTTAGATAA+CGG + chr4.2:81485161-81485180 None:intergenic 30.0%
TCAATGAGGATTACAATGTT+CGG + chr4.2:81484883-81484902 None:intergenic 30.0%
TCAGATTTATGCTACTCTAT+TGG - chr4.2:81484928-81484947 MS.gene92517:CDS 30.0%
TCTGACATTGCAAACTATAT+CGG - chr4.2:81483984-81484003 MS.gene92517:intron 30.0%
TGTAATCCTCATTGAAACAT+TGG - chr4.2:81484888-81484907 MS.gene92517:CDS 30.0%
! AATTGGTAACTCAATCAATG+AGG - chr4.2:81484484-81484503 MS.gene92517:intron 30.0%
! ATTTGTTCCATTTTGGATAC+AGG - chr4.2:81483920-81483939 MS.gene92517:intron 30.0%
!! ATTTTCGGTTTGACAATTCT+TGG - chr4.2:81485520-81485539 MS.gene92517:CDS 30.0%
!! CTCTTCAATTTGTTCCATTT+TGG - chr4.2:81483913-81483932 MS.gene92517:intron 30.0%
!! CTTGTTTCTTGATTTTCTTC+TGG + chr4.2:81484106-81484125 None:intergenic 30.0%
!!! GTCAGCAATGCATTTTTTAT+CGG - chr4.2:81484742-81484761 MS.gene92517:CDS 30.0%
!!! TATTTTTGGCATCGTATTCA+AGG + chr4.2:81484016-81484035 None:intergenic 30.0%
AAAATCCAAGAGATAGAACC+AGG - chr4.2:81483762-81483781 MS.gene92517:CDS 35.0%
AACTGTCATCCGAAGATAAA+AGG - chr4.2:81485217-81485236 MS.gene92517:intron 35.0%
AAGCTTAACAATGGGGAAAT+TGG - chr4.2:81484467-81484486 MS.gene92517:intron 35.0%
AATGACCCATGGTGTATTAT+CGG - chr4.2:81485180-81485199 MS.gene92517:intron 35.0%
AATTGAAGAGCAACTTGGTT+TGG + chr4.2:81483903-81483922 None:intergenic 35.0%
AATTGGAAAGTGAATAAGCC+TGG + chr4.2:81483783-81483802 None:intergenic 35.0%
ACTGTCATCCGAAGATAAAA+GGG - chr4.2:81485218-81485237 MS.gene92517:intron 35.0%
AGTAACCATAGAAAGCTGTA+AGG + chr4.2:81485101-81485120 None:intergenic 35.0%
ATTATATTCATGTCTTCCGG+CGG + chr4.2:81484787-81484806 None:intergenic 35.0%
CAATGTTCGGTTTATAGACA+CGG + chr4.2:81484870-81484889 None:intergenic 35.0%
CACGAATTCTCATGTAAGAA+CGG + chr4.2:81484052-81484071 None:intergenic 35.0%
CATTCACCAATGTTTCAATG+AGG + chr4.2:81484897-81484916 None:intergenic 35.0%
CTCATTGAAACATTGGTGAA+TGG - chr4.2:81484895-81484914 MS.gene92517:CDS 35.0%
GAACAAATTGAAGAGCAACT+TGG + chr4.2:81483908-81483927 None:intergenic 35.0%
GGGAATTACTGTTACTATTG+CGG - chr4.2:81484167-81484186 MS.gene92517:intron 35.0%
GTAGAAAAGCAACGGAAAAA+CGG - chr4.2:81484278-81484297 MS.gene92517:intron 35.0%
GTTACAGTTCCTAACAGTAA+CGG - chr4.2:81484359-81484378 MS.gene92517:intron 35.0%
GTTTCATGACAGAGAAGATT+GGG - chr4.2:81483961-81483980 MS.gene92517:intron 35.0%
TCATGTACCTGTATCCAAAA+TGG + chr4.2:81483930-81483949 None:intergenic 35.0%
TCTCTGTCATGAAACCTAAA+GGG + chr4.2:81483956-81483975 None:intergenic 35.0%
TTATTTGATATGCCTCTCCA+CGG - chr4.2:81485303-81485322 MS.gene92517:intron 35.0%
TTCACGAATTGATTGAGTTC+CGG + chr4.2:81485555-81485574 None:intergenic 35.0%
TTCTCTGTCATGAAACCTAA+AGG + chr4.2:81483957-81483976 None:intergenic 35.0%
TTGGCTTATTTGTGTGGAAT+CGG + chr4.2:81483683-81483702 None:intergenic 35.0%
TTTCTATGGTTACTCAGACT+CGG - chr4.2:81485107-81485126 MS.gene92517:intron 35.0%
! AACGAATTGGCTTATTTGTG+TGG + chr4.2:81483689-81483708 None:intergenic 35.0%
! ATTTTCTTCTGGTGCTTCAA+TGG + chr4.2:81484095-81484114 None:intergenic 35.0%
! CGGACTAGATCTTTAATTTC+AGG + chr4.2:81484850-81484869 None:intergenic 35.0%
! TTTTATCTTCGGATGACAGT+TGG + chr4.2:81485218-81485237 None:intergenic 35.0%
!! CATTTTTCAGTTGACCGTAT+TGG - chr4.2:81484961-81484980 MS.gene92517:CDS 35.0%
!! GTTGCGCAATAAATTTGCTA+GGG + chr4.2:81484426-81484445 None:intergenic 35.0%
!! TGTTGATAATATTGGACGGA+GGG + chr4.2:81485596-81485615 None:intergenic 35.0%
!! TTGTTGATAATATTGGACGG+AGG + chr4.2:81485597-81485616 None:intergenic 35.0%
!!! CAATTCTTGGCTTTTAGAAC+CGG - chr4.2:81485533-81485552 MS.gene92517:CDS 35.0%
AAGTCTCCGATAATACACCA+TGG + chr4.2:81485189-81485208 None:intergenic 40.0%
AAGTTCAAATACGAGCGTCT+GGG - chr4.2:81484146-81484165 MS.gene92517:intron 40.0%
AATATTGGACGGAGGGTAAT+GGG + chr4.2:81485589-81485608 None:intergenic 40.0%
AATCAATGAGGAGCTGTTAC+AGG - chr4.2:81484496-81484515 MS.gene92517:intron 40.0%
AGGTGGTTGTCAAAATACCA+TGG + chr4.2:81483846-81483865 None:intergenic 40.0%
AGTCAACAGAATTTCGCCAA+AGG + chr4.2:81485008-81485027 None:intergenic 40.0%
AGTCTCCGATAATACACCAT+GGG + chr4.2:81485188-81485207 None:intergenic 40.0%
ATCAATTCGTGAAGAGCAAC+TGG - chr4.2:81485562-81485581 MS.gene92517:CDS 40.0%
CAGATATTTCATCTTGGAGC+AGG - chr4.2:81485634-81485653 MS.gene92517:CDS 40.0%
CATGTAAGAACGGAGATGAT+TGG + chr4.2:81484042-81484061 None:intergenic 40.0%
CCCATCAAAGCTTAACAATG+GGG - chr4.2:81484460-81484479 MS.gene92517:intron 40.0%
CTACTGCGAGAAACTCTATT+CGG - chr4.2:81484211-81484230 MS.gene92517:intron 40.0%
CTGTCATCCGAAGATAAAAG+GGG - chr4.2:81485219-81485238 MS.gene92517:intron 40.0%
GCCCATCAAAGCTTAACAAT+GGG - chr4.2:81484459-81484478 MS.gene92517:intron 40.0%
GGTTTCATGACAGAGAAGAT+TGG - chr4.2:81483960-81483979 MS.gene92517:intron 40.0%
GTAAGTCCCATGATTCTCTT+CGG + chr4.2:81485137-81485156 None:intergenic 40.0%
GTGTCGCAGATATTTCATCT+TGG - chr4.2:81485628-81485647 MS.gene92517:CDS 40.0%
TAATATTGGACGGAGGGTAA+TGG + chr4.2:81485590-81485609 None:intergenic 40.0%
TACACCATGGGTCATTATAG+TGG + chr4.2:81485176-81485195 None:intergenic 40.0%
TCAAACCGAAAATTGCGGTA+GGG + chr4.2:81485513-81485532 None:intergenic 40.0%
TCAATTCGTGAAGAGCAACT+GGG - chr4.2:81485563-81485582 MS.gene92517:CDS 40.0%
TGCAGTCTTTAACAGCATCT+TGG + chr4.2:81485281-81485300 None:intergenic 40.0%
TGCTCTTGAACCATGTGAAT+TGG + chr4.2:81485332-81485351 None:intergenic 40.0%
TGGAAACAATAGAGACGTTC+TGG - chr4.2:81483721-81483740 MS.gene92517:CDS 40.0%
TGTTACTATTGCGGAATGCT+AGG - chr4.2:81484176-81484195 MS.gene92517:intron 40.0%
TTATATTCATGTCTTCCGGC+GGG + chr4.2:81484786-81484805 None:intergenic 40.0%
TTCTATGGTTACTCAGACTC+GGG - chr4.2:81485108-81485127 MS.gene92517:intron 40.0%
! AGGAGTGTGATCAGATATAG+AGG + chr4.2:81485454-81485473 None:intergenic 40.0%
! CCCCATTGTTAAGCTTTGAT+GGG + chr4.2:81484463-81484482 None:intergenic 40.0%
! GAAACTCTATTCGGTTGATG+AGG - chr4.2:81484220-81484239 MS.gene92517:intron 40.0%
! GAATTCTAAAGAAGGGTCCA+TGG - chr4.2:81483826-81483845 MS.gene92517:CDS 40.0%
! GCTAGAGCATTAGTTTCTGT+TGG + chr4.2:81484647-81484666 None:intergenic 40.0%
! GGTTGCGCAATAAATTTGCT+AGG + chr4.2:81484427-81484446 None:intergenic 40.0%
! TCCCCATTGTTAAGCTTTGA+TGG + chr4.2:81484464-81484483 None:intergenic 40.0%
! TTCACATGGTTCAAGAGCAT+CGG - chr4.2:81485333-81485352 MS.gene92517:CDS 40.0%
!! ATAAGCCTGGTTCTATCTCT+TGG + chr4.2:81483770-81483789 None:intergenic 40.0%
!! TATTCGGTTGATGAGGATGA+TGG - chr4.2:81484227-81484246 MS.gene92517:intron 40.0%
!!! TGGATTTTGACTCCTTTGAC+AGG + chr4.2:81483750-81483769 None:intergenic 40.0%
AACAAGCCAAAACAGTCCAG+AGG - chr4.2:81484545-81484564 MS.gene92517:CDS 45.0%
AAGCCTCTAATGAGCCAAGA+AGG + chr4.2:81485258-81485277 None:intergenic 45.0%
AATTTCGCCAAAGGATAGCG+AGG + chr4.2:81484999-81485018 None:intergenic 45.0%
ACAAGCCAAAACAGTCCAGA+GGG - chr4.2:81484546-81484565 MS.gene92517:CDS 45.0%
AGCTGCGTGTAGAAAAGCAA+CGG - chr4.2:81484270-81484289 MS.gene92517:intron 45.0%
AGTTCAAATACGAGCGTCTG+GGG - chr4.2:81484147-81484166 MS.gene92517:intron 45.0%
ATAATATCGTGGAACTGCCG+AGG - chr4.2:81484802-81484821 MS.gene92517:CDS 45.0%
ATCAGATATAGAGGCCACCA+AGG + chr4.2:81485445-81485464 None:intergenic 45.0%
ATTGACCTCATTCAACCCTC+TGG + chr4.2:81484564-81484583 None:intergenic 45.0%
CAAGTTCAAATACGAGCGTC+TGG - chr4.2:81484145-81484164 MS.gene92517:intron 45.0%
CAGGTACATGACATCCCTTT+AGG - chr4.2:81483939-81483958 MS.gene92517:intron 45.0%
CATCGCTGAGAGAAAGTTGA+TGG + chr4.2:81483587-81483606 None:intergenic 45.0%
CGCCCATCAAAGCTTAACAA+TGG - chr4.2:81484458-81484477 MS.gene92517:intron 45.0%
CTCTAATGAGCCAAGAAGGA+CGG + chr4.2:81485254-81485273 None:intergenic 45.0%
CTGGGATCACATTAAGAACC+AGG + chr4.2:81483869-81483888 None:intergenic 45.0%
GAAAAGCAACGGAAAAACGG+TGG - chr4.2:81484281-81484300 MS.gene92517:intron 45.0%
GGATCACATTAAGAACCAGG+AGG + chr4.2:81483866-81483885 None:intergenic 45.0%
GTAGCCACTATAATGACCCA+TGG - chr4.2:81485169-81485188 MS.gene92517:intron 45.0%
GTCAAACCGAAAATTGCGGT+AGG + chr4.2:81485514-81485533 None:intergenic 45.0%
GTGCGCAGGACTATTATCAT+TGG + chr4.2:81484687-81484706 None:intergenic 45.0%
GTGGGAGGGTTTCATCAATT+TGG + chr4.2:81483629-81483648 None:intergenic 45.0%
GTTTGGGTATAGTGATTGCC+AGG + chr4.2:81485409-81485428 None:intergenic 45.0%
TAGGGTTGTCTCCAAGGTAT+AGG + chr4.2:81485495-81485514 None:intergenic 45.0%
TCAACTTTCTCTCAGCGATG+AGG - chr4.2:81483587-81483606 MS.gene92517:CDS 45.0%
TCACATTAAGAACCAGGAGG+TGG + chr4.2:81483863-81483882 None:intergenic 45.0%
TCATCCGAAGATAAAAGGGG+TGG - chr4.2:81485222-81485241 MS.gene92517:intron 45.0%
TCCACGGTTACCAATTCACA+TGG - chr4.2:81485319-81485338 MS.gene92517:CDS 45.0%
TGACCAAAGCGCGATCAATA+CGG + chr4.2:81485380-81485399 None:intergenic 45.0%
TGCAGCTTGATCCTATACCT+TGG - chr4.2:81485481-81485500 MS.gene92517:CDS 45.0%
! ACCATGTGAATTGGTAACCG+TGG + chr4.2:81485323-81485342 None:intergenic 45.0%
! GACAACCCTACCGCAATTTT+CGG - chr4.2:81485505-81485524 MS.gene92517:CDS 45.0%
! GGTTTGGAGCAGTATTCTCT+GGG + chr4.2:81483887-81483906 None:intergenic 45.0%
! GTTGACCGTATTGGTAGAAG+CGG - chr4.2:81484970-81484989 MS.gene92517:CDS 45.0%
! TGGACCACCCCTTTTATCTT+CGG + chr4.2:81485229-81485248 None:intergenic 45.0%
! TGGTTTGGAGCAGTATTCTC+TGG + chr4.2:81483888-81483907 None:intergenic 45.0%
! TTCAACCCTCTGGACTGTTT+TGG + chr4.2:81484554-81484573 None:intergenic 45.0%
! TTTGATGGGCGCATTAAGTG+CGG + chr4.2:81484449-81484468 None:intergenic 45.0%
!! ACGTTCTGGAATCCTGTCAA+AGG - chr4.2:81483735-81483754 MS.gene92517:CDS 45.0%
!!! CATTTTTTATCGGCAGAGCC+TGG - chr4.2:81484752-81484771 MS.gene92517:CDS 45.0%
!!! GTATTTTGACAACCACCTCC+TGG - chr4.2:81483848-81483867 MS.gene92517:CDS 45.0%
!!! TCTGGACTGTTTTGGCTTGT+TGG + chr4.2:81484546-81484565 None:intergenic 45.0%
AAGCAACGGAAAAACGGTGG+AGG - chr4.2:81484284-81484303 MS.gene92517:intron 50.0%
AATACGGGCTTCCTTGGAAG+AGG + chr4.2:81485364-81485383 None:intergenic 50.0%
ACAGTCCAGAGGGTTGAATG+AGG - chr4.2:81484556-81484575 MS.gene92517:CDS 50.0%
ACTGCTCGACCGTTACTGTT+AGG + chr4.2:81484371-81484390 None:intergenic 50.0%
AGGGTCATCTGTTTGTGGGA+GGG + chr4.2:81483643-81483662 None:intergenic 50.0%
AGTCCCATGATTCTCTTCGG+CGG + chr4.2:81485134-81485153 None:intergenic 50.0%
ATACGGGCTTCCTTGGAAGA+GGG + chr4.2:81485363-81485382 None:intergenic 50.0%
ATATAGAGGCCACCAAGGTC+TGG + chr4.2:81485440-81485459 None:intergenic 50.0%
ATGATGGCACACGACTATGG+GGG - chr4.2:81484243-81484262 MS.gene92517:intron 50.0%
CGTCCTTCTTGGCTCATTAG+AGG - chr4.2:81485252-81485271 MS.gene92517:intron 50.0%
GAACTGCCTTAACATTCCCC+AGG + chr4.2:81484828-81484847 None:intergenic 50.0%
GACCAAAGCGCGATCAATAC+GGG + chr4.2:81485379-81485398 None:intergenic 50.0%
TCTCGCAGTAGTCTTCGATG+TGG + chr4.2:81484202-81484221 None:intergenic 50.0%
TGGCGCCTTACAGCTTTCTA+TGG - chr4.2:81485093-81485112 MS.gene92517:intron 50.0%
TTGTGTGGAATCGGCCAACA+AGG + chr4.2:81483674-81483693 None:intergenic 50.0%
! AGGATGATGGCACACGACTA+TGG - chr4.2:81484240-81484259 MS.gene92517:intron 50.0%
! ATTTGCTAGGGCTGCAGAGT+TGG + chr4.2:81484414-81484433 None:intergenic 50.0%
! GATGATGGCACACGACTATG+GGG - chr4.2:81484242-81484261 MS.gene92517:intron 50.0%
! GGATGATGGCACACGACTAT+GGG - chr4.2:81484241-81484260 MS.gene92517:intron 50.0%
! GTGAATTGGTAACCGTGGAG+AGG + chr4.2:81485318-81485337 None:intergenic 50.0%
! TTGATGGGCGCATTAAGTGC+GGG + chr4.2:81484448-81484467 None:intergenic 50.0%
!! GCAGAGTTGGCAGTTTTGGT+TGG + chr4.2:81484401-81484420 None:intergenic 50.0%
AGGCGAGGGTCATCTGTTTG+TGG + chr4.2:81483648-81483667 None:intergenic 55.0%
ATGAGCCAAGAAGGACGGTC+TGG + chr4.2:81485249-81485268 None:intergenic 55.0%
CAACGGAAAAACGGTGGAGG+CGG - chr4.2:81484287-81484306 MS.gene92517:intron 55.0%
CCTTAACATTCCCCAGGCCT+CGG + chr4.2:81484822-81484841 None:intergenic 55.0%
CGTCGTCAGACAGAATCTGC+GGG - chr4.2:81484314-81484333 MS.gene92517:intron 55.0%
CTGACTGCGAGACTTCCATG+GGG + chr4.2:81484727-81484746 None:intergenic 55.0%
GAGGGTCATCTGTTTGTGGG+AGG + chr4.2:81483644-81483663 None:intergenic 55.0%
GCGATCAATACGGGCTTCCT+TGG + chr4.2:81485370-81485389 None:intergenic 55.0%
GCGCTTTGGTCACATCTTCC+TGG - chr4.2:81485388-81485407 MS.gene92517:CDS 55.0%
GCGTCGTCAGACAGAATCTG+CGG - chr4.2:81484313-81484332 MS.gene92517:intron 55.0%
GCTGACTGCGAGACTTCCAT+GGG + chr4.2:81484728-81484747 None:intergenic 55.0%
GGATCAAGCTGCAGAAGCAG+AGG + chr4.2:81485474-81485493 None:intergenic 55.0%
GGCCACCGCTTCTACCAATA+CGG + chr4.2:81484978-81484997 None:intergenic 55.0%
GGCGAGGGTCATCTGTTTGT+GGG + chr4.2:81483647-81483666 None:intergenic 55.0%
GTCGTCAGACAGAATCTGCG+GGG - chr4.2:81484315-81484334 MS.gene92517:intron 55.0%
TCGTCAGACAGAATCTGCGG+GGG - chr4.2:81484316-81484335 MS.gene92517:intron 55.0%
TGTCAGTACCACTGTCCCCA+TGG - chr4.2:81484709-81484728 MS.gene92517:CDS 55.0%
TTGCGGTAGGGTTGTCTCCA+AGG + chr4.2:81485501-81485520 None:intergenic 55.0%
! AGCCCGTATTGATCGCGCTT+TGG - chr4.2:81485374-81485393 MS.gene92517:CDS 55.0%
! GACCGTATTGGTAGAAGCGG+TGG - chr4.2:81484973-81484992 MS.gene92517:CDS 55.0%
! GGCTGCAGAGTTGGCAGTTT+TGG + chr4.2:81484405-81484424 None:intergenic 55.0%
! TGCTGACTGCGAGACTTCCA+TGG + chr4.2:81484729-81484748 None:intergenic 55.0%
!! TGGGGACAGTGGTACTGACA+TGG + chr4.2:81484709-81484728 None:intergenic 55.0%
AAACGCTGCCCTCCAGACCT+TGG - chr4.2:81485425-81485444 MS.gene92517:CDS 60.0%
AAGGTCTGGAGGGCAGCGTT+TGG + chr4.2:81485426-81485445 None:intergenic 60.0%
AGAGGCCACCAAGGTCTGGA+GGG + chr4.2:81485436-81485455 None:intergenic 60.0%
AGGTCTGGAGGGCAGCGTTT+GGG + chr4.2:81485425-81485444 None:intergenic 60.0%
CATCGGCACACCCTCTTCCA+AGG - chr4.2:81485350-81485369 MS.gene92517:CDS 60.0%
CCGAGGCCTGGGGAATGTTA+AGG - chr4.2:81484819-81484838 MS.gene92517:CDS 60.0%
CGAGACTTCCATGGGGACAG+TGG + chr4.2:81484720-81484739 None:intergenic 60.0%
CGTCAGACAGAATCTGCGGG+GGG - chr4.2:81484317-81484336 MS.gene92517:intron 60.0%
GGAATCGGCCAACAAGGCAG+AGG + chr4.2:81483668-81483687 None:intergenic 60.0%
GGGCCGCCGAAGAGAATCAT+GGG - chr4.2:81485128-81485147 MS.gene92517:intron 60.0%
GTGGTCCAGACCGTCCTTCT+TGG - chr4.2:81485241-81485260 MS.gene92517:intron 60.0%
TAGAGGCCACCAAGGTCTGG+AGG + chr4.2:81485437-81485456 None:intergenic 60.0%
!! GTGGTACTGACATGGTGCGC+AGG + chr4.2:81484701-81484720 None:intergenic 60.0%
ATCGGCAGAGCCTGGTTCCC+AGG - chr4.2:81484760-81484779 MS.gene92517:CDS 65.0%
ATCGTGGAACTGCCGAGGCC+TGG - chr4.2:81484807-81484826 MS.gene92517:CDS 65.0%
CGGGCCGCCGAAGAGAATCA+TGG - chr4.2:81485127-81485146 MS.gene92517:intron 65.0%
GACCCTCGCCTCTGCCTTGT+TGG - chr4.2:81483657-81483676 MS.gene92517:CDS 65.0%
GCGGTGGCCTCGCTATCCTT+TGG - chr4.2:81484989-81485008 MS.gene92517:CDS 65.0%
GGCCAACAAGGCAGAGGCGA+GGG + chr4.2:81483662-81483681 None:intergenic 65.0%
TCATGTCTTCCGGCGGGCCT+GGG + chr4.2:81484780-81484799 None:intergenic 65.0%
TCGTGGAACTGCCGAGGCCT+GGG - chr4.2:81484808-81484827 MS.gene92517:CDS 65.0%
TGCGGGGGGAGCTTCATCAC+TGG - chr4.2:81484331-81484350 MS.gene92517:intron 65.0%
TTCATGTCTTCCGGCGGGCC+TGG + chr4.2:81484781-81484800 None:intergenic 65.0%
CGCTGCCCTCCAGACCTTGG+TGG - chr4.2:81485428-81485447 MS.gene92517:CDS 70.0%
CGGCCAACAAGGCAGAGGCG+AGG + chr4.2:81483663-81483682 None:intergenic 70.0%
CGTGGAACTGCCGAGGCCTG+GGG - chr4.2:81484809-81484828 MS.gene92517:CDS 70.0%
AGCCTGGTTCCCAGGCCCGC+CGG - chr4.2:81484768-81484787 MS.gene92517:CDS 75.0%
TTCCGGCGGGCCTGGGAACC+AGG + chr4.2:81484773-81484792 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 81483573 81485677 81483573 ID=MS.gene92517
chr4.2 mRNA 81483573 81485677 81483573 ID=MS.gene92517.t1;Parent=MS.gene92517
chr4.2 exon 81485309 81485677 81485309 ID=MS.gene92517.t1.exon1;Parent=MS.gene92517.t1
chr4.2 CDS 81485309 81485677 81485309 ID=cds.MS.gene92517.t1;Parent=MS.gene92517.t1
chr4.2 exon 81484541 81485008 81484541 ID=MS.gene92517.t1.exon2;Parent=MS.gene92517.t1
chr4.2 CDS 81484541 81485008 81484541 ID=cds.MS.gene92517.t1;Parent=MS.gene92517.t1
chr4.2 exon 81483573 81483878 81483573 ID=MS.gene92517.t1.exon3;Parent=MS.gene92517.t1
chr4.2 CDS 81483573 81483878 81483573 ID=cds.MS.gene92517.t1;Parent=MS.gene92517.t1
Gene Sequence

>MS.gene92517

ATGTCTTCTCTCCATCAACTTTCTCTCAGCGATGAGGAAAATGAATTAGAATTCCAAATTGATGAAACCCTCCCACAAACAGATGACCCTCGCCTCTGCCTTGTTGGCCGATTCCACACAAATAAGCCAATTCGTTCATATATGATGATGGAAACAATAGAGACGTTCTGGAATCCTGTCAAAGGAGTCAAAATCCAAGAGATAGAACCAGGCTTATTCACTTTCCAATTTGCACATCATCTTGATTTTCAGAGAATTCTAAAGAAGGGTCCATGGTATTTTGACAACCACCTCCTGGTTCTTAATGTGATCCCAGAGAATACTGCTCCAAACCAAGTTGCTCTTCAATTTGTTCCATTTTGGATACAGGTACATGACATCCCTTTAGGTTTCATGACAGAGAAGATTGGGTCTGACATTGCAAACTATATCGGAGAATTCCTTGAATACGATGCCAAAAATAATTCCAATCATCTCCGTTCTTACATGAGAATTCGTGTGCTGCTTGATATTACGAAGCCATTGAAGCACCAGAAGAAAATCAAGAAACAAGGAGGTGACTCAAGCTTCATCAAGTTCAAATACGAGCGTCTGGGGAATTACTGTTACTATTGCGGAATGCTAGGCCACATCGAAGACTACTGCGAGAAACTCTATTCGGTTGATGAGGATGATGGCACACGACTATGGGGGCTTGAGCTGCGTGTAGAAAAGCAACGGAAAAACGGTGGAGGCGGTGCGCGTCGTCAGACAGAATCTGCGGGGGGAGCTTCATCACTGGCAGCTGTTACAGTTCCTAACAGTAACGGTCGAGCAGTAAATGCGCCAACCAAAACTGCCAACTCTGCAGCCCTAGCAAATTTATTGCGCAACCCGCACTTAATGCGCCCATCAAAGCTTAACAATGGGGAAATTGGTAACTCAATCAATGAGGAGCTGTTACAGGAAGATAATGAAGAGCAAATCATCACCAACAAGCCAAAACAGTCCAGAGGGTTGAATGAGGTCAATCAGCAAATCATTACAATTAATCACAACTCAATACCTGTAGTTAATGAGACAGAAATTGTGCCAACAGAAACTAATGCTCTAGCAGACCAAAAATCATCTACCAATGATAATAGTCCTGCGCACCATGTCAGTACCACTGTCCCCATGGAAGTCTCGCAGTCAGCAATGCATTTTTTATCGGCAGAGCCTGGTTCCCAGGCCCGCCGGAAGACATGAATATAATATCGTGGAACTGCCGAGGCCTGGGGAATGTTAAGGCAGTTCCTGAAATTAAAGATCTAGTCCGTGTCTATAAACCGAACATTGTAATCCTCATTGAAACATTGGTGAATGGAAATAAAATTTCAGATTTATGCTACTCTATTGGTTTTGATAATCATTTTTCAGTTGACCGTATTGGTAGAAGCGGTGGCCTCGCTATCCTTTGGCGAAATTCTGTTGACTGTTCTCTCATAAATGTTTCACAAAATTTCATTAACTTGTTAATCAAAGATTCTGTTATTGGTACATGGCGCCTTACAGCTTTCTATGGTTACTCAGACTCGGGCCGCCGAAGAGAATCATGGGACTTACTCCGTTATCTAAGTAGCCACTATAATGACCCATGGTGTATTATCGGAGACTTCAACGACCAACTGTCATCCGAAGATAAAAGGGGTGGTCCAGACCGTCCTTCTTGGCTCATTAGAGGCTTCCAAGATGCTGTTAAAGACTGCAACTTATTTGATATGCCTCTCCACGGTTACCAATTCACATGGTTCAAGAGCATCGGCACACCCTCTTCCAAGGAAGCCCGTATTGATCGCGCTTTGGTCACATCTTCCTGGCAATCACTATACCCAAACGCTGCCCTCCAGACCTTGGTGGCCTCTATATCTGATCACACTCCTCTGCTTCTGCAGCTTGATCCTATACCTTGGAGACAACCCTACCGCAATTTTCGGTTTGACAATTCTTGGCTTTTAGAACCGGAACTCAATCAATTCGTGAAGAGCAACTGGGCCCATTACCCTCCGTCCAATATTATCAACAAACTCAATCACTGTGTCGCAGATATTTCATCTTGGAGCAGGAATATTGCACCAAATTTCTGA
Protein sequence

>MS.gene92517.t1

MSSLHQLSLSDEENELEFQIDETLPQTDDPRLCLVGRFHTNKPIRSYMMMETIETFWNPVKGVKIQEIEPGLFTFQFAHHLDFQRILKKGPWYFDNHLLVLNVIPENTAPNQVALQFVPFWIQDDGTRLWGLELRVEKQRKNGGGGARRQTESAGGASSLAAVTVPNSNGRAVNAPTKTANSAALANLLRNPHLMRPSKLNNGEIGNSINEELLQEDNEEQIITNKPKQSRGLNEVNQQIITINHNSIPVVNETEIVPTETNALADQKSSTNDNSPAHHEARIDRALVTSSWQSLYPNAALQTLVASISDHTPLLLQLDPIPWRQPYRNFRFDNSWLLEPELNQFVKSNWAHYPPSNIINKLNHCVADISSWSRNIAPNF