AlfalfaGEDB | MS.gene26844

M. sativa cultivar XinJiangDaYe / MS.gene26844

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26844.t1	XP_024626773.1	95.1	285	14	0	1	285	1	285	6.70E-155	556.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26844.t1	A0A2K3MPT1	80.4	285	55	1	1	285	1	284	6.3e-131	476.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene28545	MS.gene26844	PPI
MS.gene28718	MS.gene26844	PPI
MS.gene26844	MS.gene012889	PPI
MS.gene26844	MS.gene34463	PPI
MS.gene053747	MS.gene26844	PPI
MS.gene26844	MS.gene000506	PPI
MS.gene061850	MS.gene26844	PPI
MS.gene26844	MS.gene51804	PPI
MS.gene26844	MS.gene013644	PPI
MS.gene26844	MS.gene54051	PPI
MS.gene073582	MS.gene26844	PPI
MS.gene058889	MS.gene26844	PPI
MS.gene98616	MS.gene26844	PPI
MS.gene40828	MS.gene26844	PPI
MS.gene046089	MS.gene26844	PPI
MS.gene26844	MS.gene010890	PPI
MS.gene042027	MS.gene26844	PPI
MS.gene69490	MS.gene26844	PPI
MS.gene015248	MS.gene26844	PPI
MS.gene80362	MS.gene26844	PPI
MS.gene72520	MS.gene26844	PPI
MS.gene013193	MS.gene26844	PPI
MS.gene04970	MS.gene26844	PPI
MS.gene08106	MS.gene26844	PPI
MS.gene26844	MS.gene060788	PPI
MS.gene26844	MS.gene41643	PPI
MS.gene63743	MS.gene26844	PPI
MS.gene26844	MS.gene027994	PPI
MS.gene26844	MS.gene022929	PPI
MS.gene26844	MS.gene007022	PPI
MS.gene26844	MS.gene69584	PPI
MS.gene26844	MS.gene52349	PPI
MS.gene26844	MS.gene021647	PPI
MS.gene26844	MS.gene46353	PPI
MS.gene024405	MS.gene26844	PPI
MS.gene012748	MS.gene26844	PPI
MS.gene04134	MS.gene26844	PPI
MS.gene83979	MS.gene26844	PPI
MS.gene26844	MS.gene069624	PPI
MS.gene062067	MS.gene26844	PPI
MS.gene26844	MS.gene61651	PPI
MS.gene57044	MS.gene26844	PPI
MS.gene98471	MS.gene26844	PPI
MS.gene26844	MS.gene016719	PPI
MS.gene049610	MS.gene26844	PPI
MS.gene26844	MS.gene028714	PPI
MS.gene26844	MS.gene88393	PPI
MS.gene038676	MS.gene26844	PPI
MS.gene26844	MS.gene054508	PPI
MS.gene26844	MS.gene69586	PPI
MS.gene71637	MS.gene26844	PPI
MS.gene26844	MS.gene053373	PPI
MS.gene042651	MS.gene26844	PPI
MS.gene93396	MS.gene26844	PPI
MS.gene05576	MS.gene26844	PPI
MS.gene26844	MS.gene63150	PPI
MS.gene05231	MS.gene26844	PPI
MS.gene98467	MS.gene26844	PPI
MS.gene26844	MS.gene072852	PPI
MS.gene26844	MS.gene65474	PPI
MS.gene037049	MS.gene26844	PPI
MS.gene034643	MS.gene26844	PPI
MS.gene26844	MS.gene08708	PPI
MS.gene26844	MS.gene06164	PPI
MS.gene64130	MS.gene26844	PPI
MS.gene26844	MS.gene50392	PPI
MS.gene26844	MS.gene86275	PPI
MS.gene26844	MS.gene22730	PPI
MS.gene26844	MS.gene032299	PPI
MS.gene050679	MS.gene26844	PPI
MS.gene26844	MS.gene017586	PPI
MS.gene26844	MS.gene037634	PPI
MS.gene26844	MS.gene057828	PPI
MS.gene20246	MS.gene26844	PPI
MS.gene26844	MS.gene08606	PPI
MS.gene26844	MS.gene000640	PPI
MS.gene071784	MS.gene26844	PPI
MS.gene26844	MS.gene98756	PPI
MS.gene061579	MS.gene26844	PPI
MS.gene76420	MS.gene26844	PPI
MS.gene071320	MS.gene26844	PPI
MS.gene65471	MS.gene26844	PPI
MS.gene26844	MS.gene028716	PPI
MS.gene26844	MS.gene74639	PPI
MS.gene26844	MS.gene071133	PPI
MS.gene26844	MS.gene60266	PPI
MS.gene026680	MS.gene26844	PPI
MS.gene26433	MS.gene26844	PPI
MS.gene26844	MS.gene98467	PPI
MS.gene26844	MS.gene62249	PPI
MS.gene26844	MS.gene024522	PPI
MS.gene26844	MS.gene22111	PPI
MS.gene26844	MS.gene049610	PPI
MS.gene26844	MS.gene010290	PPI
MS.gene042559	MS.gene26844	PPI
MS.gene22200	MS.gene26844	PPI
MS.gene26844	MS.gene37955	PPI
MS.gene26844	MS.gene92517	PPI
MS.gene26844	MS.gene045875	PPI
MS.gene26844	MS.gene84790	PPI
MS.gene26038	MS.gene26844	PPI
MS.gene26844	MS.gene044729	PPI
MS.gene85308	MS.gene26844	PPI
MS.gene26844	MS.gene60390	PPI
MS.gene26844	MS.gene039692	PPI
MS.gene34812	MS.gene26844	PPI
MS.gene26844	MS.gene55682	PPI
MS.gene26844	MS.gene002590	PPI
MS.gene02896	MS.gene26844	PPI
MS.gene44083	MS.gene26844	PPI
MS.gene55682	MS.gene26844	PPI
MS.gene58419	MS.gene26844	PPI
MS.gene26844	MS.gene77990	PPI
MS.gene26844	MS.gene062511	PPI
MS.gene067340	MS.gene26844	PPI
MS.gene26844	MS.gene059269	PPI
MS.gene65623	MS.gene26844	PPI
MS.gene26844	MS.gene057934	PPI
MS.gene23521	MS.gene26844	PPI
MS.gene26844	MS.gene024841	PPI
MS.gene049512	MS.gene26844	PPI
MS.gene29406	MS.gene26844	PPI
MS.gene038623	MS.gene26844	PPI
MS.gene86275	MS.gene26844	PPI
MS.gene05357	MS.gene26844	PPI
MS.gene26844	MS.gene40828	PPI
MS.gene032311	MS.gene26844	PPI
MS.gene87529	MS.gene26844	PPI
MS.gene26844	MS.gene51799	PPI
MS.gene86849	MS.gene26844	PPI
MS.gene072852	MS.gene26844	PPI
MS.gene39956	MS.gene26844	PPI
MS.gene017586	MS.gene26844	PPI
MS.gene26844	MS.gene09023	PPI
MS.gene021968	MS.gene26844	PPI
MS.gene26844	MS.gene025987	PPI
MS.gene26844	MS.gene95960	PPI
MS.gene26844	MS.gene043140	PPI
MS.gene09589	MS.gene26844	PPI
MS.gene26844	MS.gene03149	PPI
MS.gene43393	MS.gene26844	PPI
MS.gene057828	MS.gene26844	PPI
MS.gene26844	MS.gene29321	PPI
MS.gene26844	MS.gene025401	PPI
MS.gene66650	MS.gene26844	PPI
MS.gene26844	MS.gene015093	PPI
MS.gene005120	MS.gene26844	PPI
MS.gene26844	MS.gene24278	PPI
MS.gene26844	MS.gene000379	PPI
MS.gene26844	MS.gene34618	PPI
MS.gene060386	MS.gene26844	PPI
MS.gene71588	MS.gene26844	PPI
MS.gene26844	MS.gene59460	PPI
MS.gene04749	MS.gene26844	PPI
MS.gene26844	MS.gene66650	PPI
MS.gene05436	MS.gene26844	PPI
MS.gene059736	MS.gene26844	PPI
MS.gene26844	MS.gene08713	PPI
MS.gene70932	MS.gene26844	PPI
MS.gene26844	MS.gene09517	PPI
MS.gene26844	MS.gene87845	PPI
MS.gene42903	MS.gene26844	PPI
MS.gene81712	MS.gene26844	PPI
MS.gene021965	MS.gene26844	PPI
MS.gene055683	MS.gene26844	PPI
MS.gene042614	MS.gene26844	PPI
MS.gene41643	MS.gene26844	PPI
MS.gene021555	MS.gene26844	PPI
MS.gene26844	MS.gene64178	PPI
MS.gene048747	MS.gene26844	PPI
MS.gene021647	MS.gene26844	PPI
MS.gene26844	MS.gene074257	PPI
MS.gene26844	MS.gene44144	PPI
MS.gene26844	MS.gene060718	PPI
MS.gene009121	MS.gene26844	PPI
MS.gene94654	MS.gene26844	PPI
MS.gene031967	MS.gene26844	PPI
MS.gene36069	MS.gene26844	PPI
MS.gene26844	MS.gene027169	PPI
MS.gene26844	MS.gene004417	PPI
MS.gene26844	MS.gene019177	PPI
MS.gene063214	MS.gene26844	PPI
MS.gene97410	MS.gene26844	PPI
MS.gene83772	MS.gene26844	PPI
MS.gene32415	MS.gene26844	PPI
MS.gene035626	MS.gene26844	PPI
MS.gene008927	MS.gene26844	PPI
MS.gene045614	MS.gene26844	PPI
MS.gene26844	MS.gene055560	PPI
MS.gene26844	MS.gene009213	PPI
MS.gene63762	MS.gene26844	PPI
MS.gene26844	MS.gene024297	PPI
MS.gene26844	MS.gene054652	PPI
MS.gene71675	MS.gene26844	PPI
MS.gene26844	MS.gene37537	PPI
MS.gene30208	MS.gene26844	PPI
MS.gene69495	MS.gene26844	PPI
MS.gene26844	MS.gene069786	PPI
MS.gene26844	MS.gene42010	PPI
MS.gene013676	MS.gene26844	PPI
MS.gene26844	MS.gene025158	PPI
MS.gene26844	MS.gene27329	PPI
MS.gene26844	MS.gene41393	PPI
MS.gene030090	MS.gene26844	PPI
MS.gene95561	MS.gene26844	PPI
MS.gene96174	MS.gene26844	PPI
MS.gene018196	MS.gene26844	PPI
MS.gene065627	MS.gene26844	PPI
MS.gene26844	MS.gene016632	PPI
MS.gene26844	MS.gene018474	PPI
MS.gene57019	MS.gene26844	PPI
MS.gene018745	MS.gene26844	PPI
MS.gene65546	MS.gene26844	PPI
MS.gene26844	MS.gene40469	PPI
MS.gene26844	MS.gene96163	PPI
MS.gene03936	MS.gene26844	PPI
MS.gene038098	MS.gene26844	PPI
MS.gene60059	MS.gene26844	PPI
MS.gene34197	MS.gene26844	PPI
MS.gene055560	MS.gene26844	PPI
MS.gene008275	MS.gene26844	PPI
MS.gene038757	MS.gene26844	PPI
MS.gene26844	MS.gene037049	PPI
MS.gene064966	MS.gene26844	PPI
MS.gene26844	MS.gene61499	PPI
MS.gene048072	MS.gene26844	PPI
MS.gene26844	MS.gene019165	PPI
MS.gene26844	MS.gene81194	PPI
MS.gene26844	MS.gene060773	PPI
MS.gene26844	MS.gene038394	PPI
MS.gene43924	MS.gene26844	PPI
MS.gene047954	MS.gene26844	PPI
MS.gene35175	MS.gene26844	PPI
MS.gene26844	MS.gene019001	PPI
MS.gene036423	MS.gene26844	PPI
MS.gene26844	MS.gene53904	PPI
MS.gene26844	MS.gene24070	PPI
MS.gene26844	MS.gene73261	PPI
MS.gene013644	MS.gene26844	PPI
MS.gene26844	MS.gene85538	PPI
MS.gene26844	MS.gene025675	PPI
MS.gene26844	MS.gene055546	PPI
MS.gene66651	MS.gene26844	PPI
MS.gene26844	MS.gene55209	PPI
MS.gene96109	MS.gene26844	PPI
MS.gene26844	MS.gene073573	PPI
MS.gene35202	MS.gene26844	PPI
MS.gene051449	MS.gene26844	PPI
MS.gene48477	MS.gene26844	PPI
MS.gene51804	MS.gene26844	PPI
MS.gene045875	MS.gene26844	PPI
MS.gene26844	MS.gene068629	PPI
MS.gene26844	MS.gene009781	PPI
MS.gene30136	MS.gene26844	PPI
MS.gene008861	MS.gene26844	PPI
MS.gene61452	MS.gene26844	PPI
MS.gene26844	MS.gene048342	PPI
MS.gene016102	MS.gene26844	PPI
MS.gene46941	MS.gene26844	PPI
MS.gene96541	MS.gene26844	PPI
MS.gene26844	MS.gene21066	PPI
MS.gene000634	MS.gene26844	PPI
MS.gene26844	MS.gene013890	PPI
MS.gene85295	MS.gene26844	PPI
MS.gene26844	MS.gene98127	PPI
MS.gene26844	MS.gene073582	PPI
MS.gene26844	MS.gene003264	PPI
MS.gene47792	MS.gene26844	PPI
MS.gene46948	MS.gene26844	PPI
MS.gene025873	MS.gene26844	PPI
MS.gene012019	MS.gene26844	PPI
MS.gene26844	MS.gene048706	PPI
MS.gene26844	MS.gene45083	PPI
MS.gene05358	MS.gene26844	PPI
MS.gene26844	MS.gene049561	PPI
MS.gene26844	MS.gene008658	PPI
MS.gene26844	MS.gene49057	PPI
MS.gene074257	MS.gene26844	PPI
MS.gene26844	MS.gene89815	PPI
MS.gene26844	MS.gene048088	PPI
MS.gene26844	MS.gene86868	PPI
MS.gene010656	MS.gene26844	PPI
MS.gene000629	MS.gene26844	PPI
MS.gene041264	MS.gene26844	PPI
MS.gene41979	MS.gene26844	PPI
MS.gene26844	MS.gene058889	PPI
MS.gene05319	MS.gene26844	PPI
MS.gene56991	MS.gene26844	PPI
MS.gene055223	MS.gene26844	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26844.t1	MTR_0010s0370	33.333	147	97	1	35	180	34	180	1.20e-25	100
MS.gene26844.t1	MTR_0010s0350	33.333	147	97	1	35	180	34	180	1.20e-25	100
MS.gene26844.t1	MTR_6g045237	26.163	172	126	1	44	214	93	264	6.32e-15	74.7
MS.gene26844.t1	MTR_6g033215	23.699	173	126	3	29	197	123	293	3.98e-13	68.6
MS.gene26844.t1	MTR_4g103530	28.723	94	67	0	54	147	1	94	2.04e-11	62.4
MS.gene26844.t1	MTR_7g106180	22.535	142	110	0	34	175	25	166	3.58e-11	62.4
MS.gene26844.t1	MTR_1g036720	26.923	104	76	0	9	112	8	111	7.79e-11	58.5
MS.gene26844.t1	MTR_2g061500	24.370	119	88	1	10	128	13	129	9.51e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26844.t1	AT3G31430	26.020	196	133	5	46	235	145	334	6.89e-16	77.0
MS.gene26844.t1	AT2G01050	23.148	216	157	5	2	214	47	256	4.57e-13	69.3

Find 84 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCTCATATGTAACTTT+TGG	0.215477	8.1:-8430821	None:intergenic
AGCTTTGAACCCCTCTTTAA+AGG	0.236360	8.1:-8430608	None:intergenic
AAGCTTATGGCCCCTGGCTT+AGG	0.245778	8.1:+8430786	MS.gene26844:CDS
CAATGAACAGAGCCTCCTTC+TGG	0.275955	8.1:+8430432	MS.gene26844:CDS
TCGCTGAAGCCATCAGTATC+AGG	0.309297	8.1:-8430746	None:intergenic
CTCATTCTCAATCGAATCTC+TGG	0.311165	8.1:+8430386	MS.gene26844:CDS
GTTTCATCTTCTTCATCAAT+AGG	0.322357	8.1:-8430113	None:intergenic
TGGACTTGAAGGAAATTAAC+AGG	0.372642	8.1:+8430540	MS.gene26844:CDS
ACTTTCAAGGGAAGGACATA+TGG	0.378660	8.1:+8430633	MS.gene26844:CDS
ATCGAAGAGGGAGGCAGAAC+AGG	0.383566	8.1:+8430325	MS.gene26844:CDS
TTTAAATCCTGTATTTCTAT+CGG	0.392579	8.1:-8430275	None:intergenic
AGCTTCTCTCGCACCCTTGT+GGG	0.397321	8.1:+8430176	MS.gene26844:CDS
TACTCTCCATGCTTGAATCA+TGG	0.403490	8.1:-8430246	None:intergenic
GCACCCTTGTGGGGAAGCTC+TGG	0.409155	8.1:+8430186	MS.gene26844:CDS
TGAGGGCATAGCGAAGAAAC+TGG	0.411505	8.1:+8430490	MS.gene26844:CDS
TTGAATCATGGTTTGTTTGA+AGG	0.414135	8.1:-8430234	None:intergenic
GGTTCAAAGCTTCACTTTCA+AGG	0.420044	8.1:+8430620	MS.gene26844:CDS
AGCTTATGGCCCCTGGCTTA+GGG	0.420583	8.1:+8430787	MS.gene26844:CDS
AATAGCAAAGGTCAGAATTC+TGG	0.433185	8.1:+8430896	MS.gene26844:CDS
AGTTACATATGAGGCGAGTA+AGG	0.440975	8.1:+8430826	MS.gene26844:CDS
ATTCTGGAACTGCAATGAAA+AGG	0.441729	8.1:+8430912	MS.gene26844:CDS
TCTTCGATGTAAACTTGAAC+AGG	0.443402	8.1:-8430310	None:intergenic
GTTTGAGGAGATGGACTTGA+AGG	0.444976	8.1:+8430529	MS.gene26844:CDS
TTAAATCCTGTATTTCTATC+GGG	0.448141	8.1:-8430274	None:intergenic
TATTGGTATGTTTGAGGAGA+TGG	0.453322	8.1:+8430520	MS.gene26844:CDS
AATGAACAGAGCCTCCTTCT+GGG	0.454868	8.1:+8430433	MS.gene26844:CDS
CCTCTTCACAATCACGCATC+TGG	0.454951	8.1:-8430715	None:intergenic
TTCTGGAACTGCAATGAAAA+GGG	0.456876	8.1:+8430913	MS.gene26844:CDS
TGATTCAAGCATGGAGAGTA+AGG	0.468346	8.1:+8430249	MS.gene26844:CDS
CTGTTCATTGCTAACTCAGA+TGG	0.468876	8.1:-8430419	None:intergenic
AGGCTTAGTGAGGTTTATAG+AGG	0.468942	8.1:-8430588	None:intergenic
AAGGAACCCGATAGAAATAC+AGG	0.470081	8.1:+8430268	MS.gene26844:CDS
GAGAGGTGACGCCCTAAGCC+AGG	0.474554	8.1:-8430798	None:intergenic
CCTCAGATCGGAGCTTCAAC+GGG	0.474752	8.1:-8430472	None:intergenic
TAACAGTATTGGTATGTTTG+AGG	0.474855	8.1:+8430514	MS.gene26844:CDS
CCTCACTAAGCCTTTAAAGA+GGG	0.479289	8.1:+8430598	MS.gene26844:CDS
CTGTCCAGAGCTTCCCCACA+AGG	0.482446	8.1:-8430190	None:intergenic
TGAAGAAGATGAAACAATTA+CGG	0.486456	8.1:+8430121	MS.gene26844:CDS
AGAGGGAGGCAGAACAGGTA+TGG	0.507537	8.1:+8430330	MS.gene26844:CDS
CTTTCAAGGGAAGGACATAT+GGG	0.514821	8.1:+8430634	MS.gene26844:CDS
TATTGCTGTGTGAGGGAAAG+AGG	0.522738	8.1:-8430877	None:intergenic
CCCGTTGAAGCTCCGATCTG+AGG	0.524721	8.1:+8430472	MS.gene26844:CDS
ACCTTTGCTATTGCTGTGTG+AGG	0.526767	8.1:-8430885	None:intergenic
TTGAAGGCTCTTATGTTGAA+AGG	0.528198	8.1:-8430218	None:intergenic
AAGCTTCTCTCGCACCCTTG+TGG	0.533941	8.1:+8430175	MS.gene26844:CDS
ACCTCACTAAGCCTTTAAAG+AGG	0.534814	8.1:+8430597	MS.gene26844:CDS
CCCTCAGATCGGAGCTTCAA+CGG	0.534889	8.1:-8430473	None:intergenic
GTGGGGAAGCTCTGGACAGA+GGG	0.535227	8.1:+8430194	MS.gene26844:CDS
AAGAAACTGGGTAACAGTAT+TGG	0.537054	8.1:+8430503	MS.gene26844:CDS
CGCTGAAGCCATCAGTATCA+GGG	0.538410	8.1:-8430745	None:intergenic
TAAGCCAGGGGCCATAAGCT+TGG	0.544919	8.1:-8430784	None:intergenic
AAGACCAAGCTTATGGCCCC+TGG	0.547733	8.1:+8430780	MS.gene26844:CDS
AAGTTCCTGTCAAAACTCCA+TGG	0.549530	8.1:-8430362	None:intergenic
AAAGCTTCACTTTCAAGGGA+AGG	0.550693	8.1:+8430625	MS.gene26844:CDS
CTTGAAGGAAATTAACAGGA+TGG	0.550924	8.1:+8430544	MS.gene26844:CDS
CCGTTGAAGCTCCGATCTGA+GGG	0.551511	8.1:+8430473	MS.gene26844:CDS
AGAGGTGACGCCCTAAGCCA+GGG	0.553434	8.1:-8430797	None:intergenic
TCTCCCAAAAGTTACATATG+AGG	0.555297	8.1:+8430817	MS.gene26844:CDS
GTTCAAAGCTTCACTTTCAA+GGG	0.557197	8.1:+8430621	MS.gene26844:CDS
TTGAAGGAAATTAACAGGAT+GGG	0.574698	8.1:+8430545	MS.gene26844:CDS
TCATTCTCAATCGAATCTCT+GGG	0.580533	8.1:+8430387	MS.gene26844:CDS
CCCTCTTTAAAGGCTTAGTG+AGG	0.586504	8.1:-8430598	None:intergenic
TCTTCGCTATGCCCTCAGAT+CGG	0.586864	8.1:-8430484	None:intergenic
AACAAACCATGATTCAAGCA+TGG	0.590534	8.1:+8430240	MS.gene26844:CDS
GTTCAAGTTTACATCGAAGA+GGG	0.595924	8.1:+8430313	MS.gene26844:CDS
GCTTCTCTCGCACCCTTGTG+GGG	0.597188	8.1:+8430177	MS.gene26844:CDS
ATCATAGACACGAACCCAGA+AGG	0.597266	8.1:-8430447	None:intergenic
TGTCCAGAGCTTCCCCACAA+GGG	0.602010	8.1:-8430189	None:intergenic
CTCACTAAGCCTTTAAAGAG+GGG	0.613862	8.1:+8430599	MS.gene26844:CDS
TGTTCAAGTTTACATCGAAG+AGG	0.624773	8.1:+8430312	MS.gene26844:CDS
GAGGGCATAGCGAAGAAACT+GGG	0.625715	8.1:+8430491	MS.gene26844:CDS
CAAGTTTACATCGAAGAGGG+AGG	0.626257	8.1:+8430316	MS.gene26844:CDS
AGTTCCTGTCAAAACTCCAT+GGG	0.629838	8.1:-8430361	None:intergenic
TGTGGGGAAGCTCTGGACAG+AGG	0.631343	8.1:+8430193	MS.gene26844:CDS
CCCTCACACAGCAATAGCAA+AGG	0.634211	8.1:+8430884	MS.gene26844:CDS
CCTTTGCTATTGCTGTGTGA+GGG	0.636695	8.1:-8430884	None:intergenic
TGGAACTGCAATGAAAAGGG+AGG	0.637127	8.1:+8430916	MS.gene26844:CDS
CAGATGCGTGATTGTGAAGA+GGG	0.637335	8.1:+8430716	MS.gene26844:CDS
GGTGAAAACCCTGATACTGA+TGG	0.640035	8.1:+8430737	MS.gene26844:CDS
TGATGGCTTCAGCGAACTCG+AGG	0.649597	8.1:+8430754	MS.gene26844:CDS
GAGGTGACGCCCTAAGCCAG+GGG	0.650180	8.1:-8430796	None:intergenic
CCAGATGCGTGATTGTGAAG+AGG	0.653312	8.1:+8430715	MS.gene26844:CDS
AGAAGATGAAACAATTACGG+TGG	0.679286	8.1:+8430124	MS.gene26844:CDS
ATAGACACGAACCCAGAAGG+AGG	0.690252	8.1:-8430444	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTAAATCCTGTATTTCTAT+CGG	-	chr8.1:8430278-8430297	None:intergenic	20.0%
!	TGAAGAAGATGAAACAATTA+CGG	+	chr8.1:8430121-8430140	MS.gene26844:CDS	25.0%
!!	TTAAATCCTGTATTTCTATC+GGG	-	chr8.1:8430277-8430296	None:intergenic	25.0%
!!!	CAAATTTTTGTTTTGCTTGT+GGG	+	chr8.1:8430681-8430700	MS.gene26844:CDS	25.0%
	CCACAAGCAAAACAAAAATT+TGG	-	chr8.1:8430683-8430702	None:intergenic	30.0%
	GTTTCATCTTCTTCATCAAT+AGG	-	chr8.1:8430116-8430135	None:intergenic	30.0%
	TTGAAGGAAATTAACAGGAT+GGG	+	chr8.1:8430545-8430564	MS.gene26844:CDS	30.0%
!!	TAACAGTATTGGTATGTTTG+AGG	+	chr8.1:8430514-8430533	MS.gene26844:CDS	30.0%
!!	TTGAATCATGGTTTGTTTGA+AGG	-	chr8.1:8430237-8430256	None:intergenic	30.0%
!!!	CCAAATTTTTGTTTTGCTTG+TGG	+	chr8.1:8430680-8430699	MS.gene26844:CDS	30.0%
	AACAAACCATGATTCAAGCA+TGG	+	chr8.1:8430240-8430259	MS.gene26844:CDS	35.0%
	AAGAAACTGGGTAACAGTAT+TGG	+	chr8.1:8430503-8430522	MS.gene26844:CDS	35.0%
	AATAGCAAAGGTCAGAATTC+TGG	+	chr8.1:8430896-8430915	MS.gene26844:CDS	35.0%
	AGAAGATGAAACAATTACGG+TGG	+	chr8.1:8430124-8430143	MS.gene26844:CDS	35.0%
	CTTGAAGGAAATTAACAGGA+TGG	+	chr8.1:8430544-8430563	MS.gene26844:CDS	35.0%
	GTTCAAAGCTTCACTTTCAA+GGG	+	chr8.1:8430621-8430640	MS.gene26844:CDS	35.0%
	TATTGGTATGTTTGAGGAGA+TGG	+	chr8.1:8430520-8430539	MS.gene26844:CDS	35.0%
	TCATTCTCAATCGAATCTCT+GGG	+	chr8.1:8430387-8430406	MS.gene26844:CDS	35.0%
	TCTCCCAAAAGTTACATATG+AGG	+	chr8.1:8430817-8430836	MS.gene26844:CDS	35.0%
	TCTTCGATGTAAACTTGAAC+AGG	-	chr8.1:8430313-8430332	None:intergenic	35.0%
	TGGACTTGAAGGAAATTAAC+AGG	+	chr8.1:8430540-8430559	MS.gene26844:CDS	35.0%
	TTGAAGGCTCTTATGTTGAA+AGG	-	chr8.1:8430221-8430240	None:intergenic	35.0%
!	GTTCAAGTTTACATCGAAGA+GGG	+	chr8.1:8430313-8430332	MS.gene26844:CDS	35.0%
!	TCGCCTCATATGTAACTTTT+GGG	-	chr8.1:8430823-8430842	None:intergenic	35.0%
!	TGTTCAAGTTTACATCGAAG+AGG	+	chr8.1:8430312-8430331	MS.gene26844:CDS	35.0%
!!	ATTCTGGAACTGCAATGAAA+AGG	+	chr8.1:8430912-8430931	MS.gene26844:CDS	35.0%
!!	TCATATGTAACTTTTGGGAG+AGG	-	chr8.1:8430818-8430837	None:intergenic	35.0%
!!	TTCTGGAACTGCAATGAAAA+GGG	+	chr8.1:8430913-8430932	MS.gene26844:CDS	35.0%
!!!	ATTTTTGTTTTGCTTGTGGG+AGG	+	chr8.1:8430684-8430703	MS.gene26844:CDS	35.0%
	AAAGCTTCACTTTCAAGGGA+AGG	+	chr8.1:8430625-8430644	MS.gene26844:CDS	40.0%
	AAGGAACCCGATAGAAATAC+AGG	+	chr8.1:8430268-8430287	MS.gene26844:CDS	40.0%
	AAGTTCCTGTCAAAACTCCA+TGG	-	chr8.1:8430365-8430384	None:intergenic	40.0%
	ACTTTCAAGGGAAGGACATA+TGG	+	chr8.1:8430633-8430652	MS.gene26844:CDS	40.0%
	AGGCTTAGTGAGGTTTATAG+AGG	-	chr8.1:8430591-8430610	None:intergenic	40.0%
	AGTTACATATGAGGCGAGTA+AGG	+	chr8.1:8430826-8430845	MS.gene26844:CDS	40.0%
	AGTTCCTGTCAAAACTCCAT+GGG	-	chr8.1:8430364-8430383	None:intergenic	40.0%
	CTCATTCTCAATCGAATCTC+TGG	+	chr8.1:8430386-8430405	MS.gene26844:CDS	40.0%
	CTGTTCATTGCTAACTCAGA+TGG	-	chr8.1:8430422-8430441	None:intergenic	40.0%
	CTTTCAAGGGAAGGACATAT+GGG	+	chr8.1:8430634-8430653	MS.gene26844:CDS	40.0%
	GAGGAAAAAGACCAAGCTTA+TGG	+	chr8.1:8430773-8430792	MS.gene26844:CDS	40.0%
	GCAGAACAGGTATGGAAAAA+TGG	+	chr8.1:8430338-8430357	MS.gene26844:CDS	40.0%
	GGTTCAAAGCTTCACTTTCA+AGG	+	chr8.1:8430620-8430639	MS.gene26844:CDS	40.0%
	TACTCTCCATGCTTGAATCA+TGG	-	chr8.1:8430249-8430268	None:intergenic	40.0%
	TGATTCAAGCATGGAGAGTA+AGG	+	chr8.1:8430249-8430268	MS.gene26844:CDS	40.0%
!	ACCTCACTAAGCCTTTAAAG+AGG	+	chr8.1:8430597-8430616	MS.gene26844:CDS	40.0%
!	CCTCACTAAGCCTTTAAAGA+GGG	+	chr8.1:8430598-8430617	MS.gene26844:CDS	40.0%
!	CTCACTAAGCCTTTAAAGAG+GGG	+	chr8.1:8430599-8430618	MS.gene26844:CDS	40.0%
!	CTCGCCTCATATGTAACTTT+TGG	-	chr8.1:8430824-8430843	None:intergenic	40.0%
!!	AGCTTTGAACCCCTCTTTAA+AGG	-	chr8.1:8430611-8430630	None:intergenic	40.0%
	AATGAACAGAGCCTCCTTCT+GGG	+	chr8.1:8430433-8430452	MS.gene26844:CDS	45.0%
	AGGTATGGAAAAATGGCCCA+TGG	+	chr8.1:8430345-8430364	MS.gene26844:CDS	45.0%
	ATCATAGACACGAACCCAGA+AGG	-	chr8.1:8430450-8430469	None:intergenic	45.0%
	CAAGTTTACATCGAAGAGGG+AGG	+	chr8.1:8430316-8430335	MS.gene26844:CDS	45.0%
	CAGATGCGTGATTGTGAAGA+GGG	+	chr8.1:8430716-8430735	MS.gene26844:CDS	45.0%
	GGTGAAAACCCTGATACTGA+TGG	+	chr8.1:8430737-8430756	MS.gene26844:CDS	45.0%
	TATTGCTGTGTGAGGGAAAG+AGG	-	chr8.1:8430880-8430899	None:intergenic	45.0%
	TGGAACTGCAATGAAAAGGG+AGG	+	chr8.1:8430916-8430935	MS.gene26844:CDS	45.0%
!	CCCTCTTTAAAGGCTTAGTG+AGG	-	chr8.1:8430601-8430620	None:intergenic	45.0%
!	GTTTGAGGAGATGGACTTGA+AGG	+	chr8.1:8430529-8430548	MS.gene26844:CDS	45.0%
!	TGTTTTGCTTGTGGGAGGAT+AGG	+	chr8.1:8430689-8430708	MS.gene26844:CDS	45.0%
!!	ACCTTTGCTATTGCTGTGTG+AGG	-	chr8.1:8430888-8430907	None:intergenic	45.0%
!!	CCTTTGCTATTGCTGTGTGA+GGG	-	chr8.1:8430887-8430906	None:intergenic	45.0%
	ATAGACACGAACCCAGAAGG+AGG	-	chr8.1:8430447-8430466	None:intergenic	50.0%
	CAATGAACAGAGCCTCCTTC+TGG	+	chr8.1:8430432-8430451	MS.gene26844:CDS	50.0%
	CCAGATGCGTGATTGTGAAG+AGG	+	chr8.1:8430715-8430734	MS.gene26844:CDS	50.0%
	CCCTCACACAGCAATAGCAA+AGG	+	chr8.1:8430884-8430903	MS.gene26844:CDS	50.0%
	CCTCTTCACAATCACGCATC+TGG	-	chr8.1:8430718-8430737	None:intergenic	50.0%
	CGCTGAAGCCATCAGTATCA+GGG	-	chr8.1:8430748-8430767	None:intergenic	50.0%
	TCGCTGAAGCCATCAGTATC+AGG	-	chr8.1:8430749-8430768	None:intergenic	50.0%
	TCTTCGCTATGCCCTCAGAT+CGG	-	chr8.1:8430487-8430506	None:intergenic	50.0%
!	GAGGGCATAGCGAAGAAACT+GGG	+	chr8.1:8430491-8430510	MS.gene26844:CDS	50.0%
!	GGGAAAGAGGTTTTTGCTGC+AGG	-	chr8.1:8430867-8430886	None:intergenic	50.0%
!	TGAGGGCATAGCGAAGAAAC+TGG	+	chr8.1:8430490-8430509	MS.gene26844:CDS	50.0%
!!	ATGGCCCATGGAGTTTTGAC+AGG	+	chr8.1:8430357-8430376	MS.gene26844:CDS	50.0%
	AAGACCAAGCTTATGGCCCC+TGG	+	chr8.1:8430780-8430799	MS.gene26844:CDS	55.0%
	AAGCTTATGGCCCCTGGCTT+AGG	+	chr8.1:8430786-8430805	MS.gene26844:CDS	55.0%
	AAGCTTCTCTCGCACCCTTG+TGG	+	chr8.1:8430175-8430194	MS.gene26844:CDS	55.0%
	AGAGGGAGGCAGAACAGGTA+TGG	+	chr8.1:8430330-8430349	MS.gene26844:CDS	55.0%
	AGCTTATGGCCCCTGGCTTA+GGG	+	chr8.1:8430787-8430806	MS.gene26844:CDS	55.0%
	AGCTTCTCTCGCACCCTTGT+GGG	+	chr8.1:8430176-8430195	MS.gene26844:CDS	55.0%
	ATCGAAGAGGGAGGCAGAAC+AGG	+	chr8.1:8430325-8430344	MS.gene26844:CDS	55.0%
	CCCTCAGATCGGAGCTTCAA+CGG	-	chr8.1:8430476-8430495	None:intergenic	55.0%
	CCGTTGAAGCTCCGATCTGA+GGG	+	chr8.1:8430473-8430492	MS.gene26844:CDS	55.0%
	CCTCAGATCGGAGCTTCAAC+GGG	-	chr8.1:8430475-8430494	None:intergenic	55.0%
	TAAGCCAGGGGCCATAAGCT+TGG	-	chr8.1:8430787-8430806	None:intergenic	55.0%
	TGATGGCTTCAGCGAACTCG+AGG	+	chr8.1:8430754-8430773	MS.gene26844:CDS	55.0%
	TGTCCAGAGCTTCCCCACAA+GGG	-	chr8.1:8430192-8430211	None:intergenic	55.0%
	AGAGGTGACGCCCTAAGCCA+GGG	-	chr8.1:8430800-8430819	None:intergenic	60.0%
	CCCGTTGAAGCTCCGATCTG+AGG	+	chr8.1:8430472-8430491	MS.gene26844:CDS	60.0%
	CTGTCCAGAGCTTCCCCACA+AGG	-	chr8.1:8430193-8430212	None:intergenic	60.0%
	GCTTCTCTCGCACCCTTGTG+GGG	+	chr8.1:8430177-8430196	MS.gene26844:CDS	60.0%
!!	GTGGGGAAGCTCTGGACAGA+GGG	+	chr8.1:8430194-8430213	MS.gene26844:CDS	60.0%
!!	TGTGGGGAAGCTCTGGACAG+AGG	+	chr8.1:8430193-8430212	MS.gene26844:CDS	60.0%
	GAGAGGTGACGCCCTAAGCC+AGG	-	chr8.1:8430801-8430820	None:intergenic	65.0%
	GAGGTGACGCCCTAAGCCAG+GGG	-	chr8.1:8430799-8430818	None:intergenic	65.0%
	GCACCCTTGTGGGGAAGCTC+TGG	+	chr8.1:8430186-8430205	MS.gene26844:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	8430089	8430946	8430089	ID=MS.gene26844
chr8.1	mRNA	8430089	8430946	8430089	ID=MS.gene26844.t1;Parent=MS.gene26844
chr8.1	exon	8430089	8430946	8430089	ID=MS.gene26844.t1.exon1;Parent=MS.gene26844.t1
chr8.1	CDS	8430089	8430946	8430089	ID=cds.MS.gene26844.t1;Parent=MS.gene26844.t1

Gene Sequence

>MS.gene26844

ATGGAGAGACAAAAATCGAAGCAACCTATTGATGAAGAAGATGAAACAATTACGGTGGAAGCTGATGAGATGTGCAATGATGATGAAAGCTTCTCTCGCACCCTTGTGGGGAAGCTCTGGACAGAGGGACCTTTCAACATAAGAGCCTTCAAACAAACCATGATTCAAGCATGGAGAGTAAGGAACCCGATAGAAATACAGGATTTAAACAAGAACATGTTCCTGTTCAAGTTTACATCGAAGAGGGAGGCAGAACAGGTATGGAAAAATGGCCCATGGAGTTTTGACAGGAACTTACTCATTCTCAATCGAATCTCTGGGAATGAACAACCATCTGAGTTAGCAATGAACAGAGCCTCCTTCTGGGTTCGTGTCTATGATCTCCCGTTGAAGCTCCGATCTGAGGGCATAGCGAAGAAACTGGGTAACAGTATTGGTATGTTTGAGGAGATGGACTTGAAGGAAATTAACAGGATGGGAAAATTTCTACGACTCAGAGCCTCTATAAACCTCACTAAGCCTTTAAAGAGGGGTTCAAAGCTTCACTTTCAAGGGAAGGACATATGGGTTGACTACAAGTATGAAAGATTGCCAAATTTTTGTTTTGCTTGTGGGAGGATAGGACACCAGATGCGTGATTGTGAAGAGGGTGAAAACCCTGATACTGATGGCTTCAGCGAACTCGAGGAAAAAGACCAAGCTTATGGCCCCTGGCTTAGGGCGTCACCTCTCCCAAAAGTTACATATGAGGCGAGTAAGGAATCAAGTTCTAGTGCCTGCAGCAAAAACCTCTTTCCCTCACACAGCAATAGCAAAGGTCAGAATTCTGGAACTGCAATGAAAAGGGAGGAAGAATGA

Protein sequence

>MS.gene26844.t1

MERQKSKQPIDEEDETITVEADEMCNDDESFSRTLVGKLWTEGPFNIRAFKQTMIQAWRVRNPIEIQDLNKNMFLFKFTSKREAEQVWKNGPWSFDRNLLILNRISGNEQPSELAMNRASFWVRVYDLPLKLRSEGIAKKLGNSIGMFEEMDLKEINRMGKFLRLRASINLTKPLKRGSKLHFQGKDIWVDYKYERLPNFCFACGRIGHQMRDCEEGENPDTDGFSELEEKDQAYGPWLRASPLPKVTYEASKESSSSACSKNLFPSHSNSKGQNSGTAMKREEE