AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65546

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65546.t1 MTR_0010s0370 55.484 155 68 1 6 159 33 187 9.17e-57 181
MS.gene65546.t1 MTR_0010s0350 55.484 155 68 1 6 159 33 187 9.17e-57 181
MS.gene65546.t1 MTR_6g033215 27.879 165 118 1 8 171 130 294 3.13e-24 99.8
MS.gene65546.t1 MTR_3g053920 33.758 157 86 3 8 163 37 176 9.08e-23 95.1
MS.gene65546.t1 MTR_1g036720 52.632 76 36 0 8 83 35 110 1.89e-22 90.1
MS.gene65546.t1 MTR_7g089990 45.455 110 57 3 75 181 84 193 1.08e-21 90.9
MS.gene65546.t1 MTR_2g061500 41.758 91 53 0 8 98 37 127 1.62e-20 85.9
MS.gene65546.t1 MTR_4g103530 40.909 88 52 0 31 118 6 93 1.01e-16 77.0
MS.gene65546.t1 MTR_1g036710 56.923 65 25 2 105 167 1 64 2.68e-15 74.7
MS.gene65546.t1 MTR_7g106180 25.517 145 101 2 8 149 27 167 2.06e-13 68.6
MS.gene65546.t1 MTR_3g024240 33.058 121 78 1 55 172 55 175 1.37e-12 65.5
MS.gene65546.t1 MTR_7g011710 36.486 74 47 0 40 113 15 88 4.62e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65546.t1 AT3G31430 26.984 189 129 4 2 187 129 311 9.17e-15 73.9

Find 72 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTGAGAGGAACAGATTT+TGG 0.248387 6.4:-5939517 None:intergenic
TAATTTCGTTGGCGAATATA+TGG 0.267133 6.4:+5939612 MS.gene65546:CDS
CTCTCAATCATATACCCTTC+TGG 0.292054 6.4:+5939530 MS.gene65546:CDS
ATGGAGCACCTTTCCATTGC+AGG 0.293915 6.4:+5939193 MS.gene65546:CDS
CATATAAGAAGCAAGAAAAT+TGG 0.294860 6.4:-5939656 None:intergenic
GTCTTTCGACGGGCATCTTC+TGG 0.300199 6.4:+5939465 MS.gene65546:CDS
TGTTTGTCAGATGTCTATTA+AGG 0.315865 6.4:+5939366 MS.gene65546:CDS
CGCTCAAGGAGTACTGATTC+TGG 0.328449 6.4:+5940180 MS.gene65546:CDS
CATTACTGCAGGTAAACAAT+TGG 0.385387 6.4:+5939588 MS.gene65546:CDS
CTGATGATGGCACACGCCTT+TGG 0.408898 6.4:+5939854 MS.gene65546:CDS
TTAATAGACATCTGACAAAC+AGG 0.416021 6.4:-5939364 None:intergenic
TCTCAGGGTCCAGCTTTAGA+AGG 0.418244 6.4:-5941029 None:intergenic
TTGATGGAAGAAGAATTAAA+TGG 0.421284 6.4:+5940144 MS.gene65546:CDS
CAATTGTTTACCTGCAGTAA+TGG 0.424201 6.4:-5939587 None:intergenic
TCCACTACCATTTCTCTTCA+CGG 0.442581 6.4:-5939896 None:intergenic
TGTCACGAGGGTGAAAGAAC+TGG 0.445172 6.4:-5939414 None:intergenic
TTCATCCTCTTCACCTGCAA+TGG 0.458106 6.4:-5939206 None:intergenic
TGTAAACGAGACTGAATTGA+TGG 0.463122 6.4:+5940128 MS.gene65546:CDS
CCACTACCATTTCTCTTCAC+GGG 0.471601 6.4:-5939895 None:intergenic
GAGGGTGAAAGAACTGGAAT+AGG 0.478785 6.4:-5939408 None:intergenic
GACGGGCATCTTCTGGTACT+AGG 0.483190 6.4:+5939472 MS.gene65546:CDS
AAACAATTGGGTAATTTCGT+TGG 0.483679 6.4:+5939601 MS.gene65546:CDS
ATTACTGCAGGTAAACAATT+GGG 0.492640 6.4:+5939589 MS.gene65546:CDS
TATGAGTATTCGTGTTATGC+TGG 0.498672 6.4:+5939675 MS.gene65546:CDS
AATTCAGTCTCGTTTACATC+CGG 0.500067 6.4:-5940123 None:intergenic
GTTCTTTCACCCTCGTGACA+TGG 0.508138 6.4:+5939417 MS.gene65546:CDS
CGTTTCAAATATGAGAGACT+CGG 0.513144 6.4:+5939760 MS.gene65546:CDS
TAATGGACATACAGCCGATC+GGG 0.515789 6.4:-5939570 None:intergenic
ATCCGGAGTTAAATTTGACT+TGG 0.524266 6.4:-5940106 None:intergenic
TATGCTAATGCTACTGATGA+TGG 0.526960 6.4:+5939841 MS.gene65546:CDS
TACAGCCGATCGGGACATCA+TGG 0.529284 6.4:-5939561 None:intergenic
GGCTGTATGTCCATTACTGC+AGG 0.529682 6.4:+5939577 MS.gene65546:CDS
GCTGGATGTTCGAAAACCAT+TGG 0.532831 6.4:+5939693 MS.gene65546:CDS
AAGGTCCATGGTCTTTCGAC+GGG 0.535853 6.4:+5939455 MS.gene65546:CDS
GGGTCCAGCTTTAGAAGGAT+AGG 0.540824 6.4:-5941024 None:intergenic
GAAAGAACTGGAATAGGTAC+AGG 0.541774 6.4:-5939402 None:intergenic
TTGACTTGGTTGTCGAGGGA+GGG 0.543322 6.4:-5940092 None:intergenic
CACTACCATTTCTCTTCACG+GGG 0.544095 6.4:-5939894 None:intergenic
ATTTGTGAACGCCTCAGCCA+TGG 0.545230 6.4:-5940055 None:intergenic
AAATTTGACTTGGTTGTCGA+GGG 0.545829 6.4:-5940096 None:intergenic
TAAATTTGACTTGGTTGTCG+AGG 0.558241 6.4:-5940097 None:intergenic
GAGGGAGGGAATAAAGTAGC+AGG 0.561743 6.4:-5940078 None:intergenic
CTCGAGGACGCATATCTCCA+TGG 0.562361 6.4:+5940038 MS.gene65546:CDS
ATCATGGACTTGCAACCAGA+AGG 0.569010 6.4:-5939545 None:intergenic
TTTGACTTGGTTGTCGAGGG+AGG 0.572535 6.4:-5940093 None:intergenic
CCTCTTCACCTGCAATGGAA+AGG 0.574754 6.4:-5939201 None:intergenic
TTGTCAGATGTCTATTAAGG+AGG 0.578781 6.4:+5939369 MS.gene65546:CDS
TGCGATGAAGGAGAAGATGC+CGG 0.583018 6.4:+5939330 MS.gene65546:CDS
TATTCGATTCTATGCGATGA+AGG 0.583832 6.4:+5939318 MS.gene65546:CDS
AAAGGTCCATGGTCTTTCGA+CGG 0.589518 6.4:+5939454 MS.gene65546:CDS
GTTTCAAATATGAGAGACTC+GGG 0.590547 6.4:+5939761 MS.gene65546:CDS
AAACGAACTTCATGTGAACC+AGG 0.592349 6.4:-5939742 None:intergenic
ATGGAGCGAGTACTCAAGAA+AGG 0.596060 6.4:+5939436 MS.gene65546:CDS
CAAGTCCATGATGTCCCGAT+CGG 0.598445 6.4:+5939556 MS.gene65546:CDS
GACGCATATCTCCATGGCTG+AGG 0.599342 6.4:+5940044 MS.gene65546:CDS
CACGTAGCTCAGAACTCCAA+AGG 0.602975 6.4:-5939870 None:intergenic
CAGAAGGGTATATGATTGAG+AGG 0.604011 6.4:-5939529 None:intergenic
AGTACTCGCTCCATGTCACG+AGG 0.611590 6.4:-5939427 None:intergenic
TGATTCTGGAACTGCACCAA+AGG 0.614269 6.4:+5940194 MS.gene65546:CDS
TCATGGACTTGCAACCAGAA+GGG 0.615325 6.4:-5939544 None:intergenic
CAAACAGGTTTCATGAACTC+CGG 0.621481 6.4:-5939349 None:intergenic
AGATGCCCGTCGAAAGACCA+TGG 0.621741 6.4:-5939460 None:intergenic
AACCAAGTCAAATTTAACTC+CGG 0.622858 6.4:+5940104 MS.gene65546:CDS
GTAATGGACATACAGCCGAT+CGG 0.626552 6.4:-5939571 None:intergenic
CAATAGTAATTAATAACTCG+AGG 0.651706 6.4:+5940022 MS.gene65546:intron
GAGTACTCAAGAAAGGTCCA+TGG 0.653102 6.4:+5939443 MS.gene65546:CDS
ATAGAATCGAATACGACGGT+CGG 0.657359 6.4:-5939308 None:intergenic
GATAGGTGAATGATCTGAAG+TGG 0.662753 6.4:-5941007 None:intergenic
TGCTGCATAATTCATCTGTG+TGG 0.683492 6.4:-5939813 None:intergenic
TCGCATAGAATCGAATACGA+CGG 0.740258 6.4:-5939312 None:intergenic
GTACTCGCTCCATGTCACGA+GGG 0.761679 6.4:-5939426 None:intergenic
ACTACCATTTCTCTTCACGG+GGG 0.765148 6.4:-5939893 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTCTTAATCTTTTTAAAT+CGG - chr6.4:5939720-5939739 None:intergenic 15.0%
!! AACTTGCAAATTAATGAAAT+TGG - chr6.4:5940605-5940624 None:intergenic 20.0%
!! AGATTAATTAATCTTGCATT+CGG - chr6.4:5940979-5940998 None:intergenic 20.0%
!! TATAATATTACATTGGATAG+AGG - chr6.4:5940572-5940591 None:intergenic 20.0%
!! TTCAATAAGGAAAAAATATC+CGG - chr6.4:5940437-5940456 None:intergenic 20.0%
! CAATAGTAATTAATAACTCG+AGG + chr6.4:5940022-5940041 MS.gene65546:intron 25.0%
! CATATAAGAAGCAAGAAAAT+TGG - chr6.4:5939659-5939678 None:intergenic 25.0%
! CTCGAGTTATTAATTACTAT+TGG - chr6.4:5940024-5940043 None:intergenic 25.0%
! GATTAATTAATCTTGCATTC+GGG - chr6.4:5940978-5940997 None:intergenic 25.0%
! TTGATGGAAGAAGAATTAAA+TGG + chr6.4:5940144-5940163 MS.gene65546:CDS 25.0%
! TTGCAAATTAATGAAATTGG+AGG - chr6.4:5940602-5940621 None:intergenic 25.0%
!! ACGAAATGAGTATTTTATGT+TGG + chr6.4:5940345-5940364 MS.gene65546:intron 25.0%
AAAAAGATTAAGACTAAGCC+TGG + chr6.4:5939724-5939743 MS.gene65546:CDS 30.0%
AAACAATTGGGTAATTTCGT+TGG + chr6.4:5939601-5939620 MS.gene65546:CDS 30.0%
AAATCACCTATAATACACCA+AGG - chr6.4:5940742-5940761 None:intergenic 30.0%
AACCAAGTCAAATTTAACTC+CGG + chr6.4:5940104-5940123 MS.gene65546:CDS 30.0%
AATCACCTATAATACACCAA+GGG - chr6.4:5940741-5940760 None:intergenic 30.0%
AGTGAGACAAAGTTTCAATA+AGG - chr6.4:5940450-5940469 None:intergenic 30.0%
ATTACTGCAGGTAAACAATT+GGG + chr6.4:5939589-5939608 MS.gene65546:CDS 30.0%
CAACCCACAAAAATAACAAA+AGG - chr6.4:5939788-5939807 None:intergenic 30.0%
TAAAAGGATATCCTTCTAGA+GGG - chr6.4:5940870-5940889 None:intergenic 30.0%
TAATTTCGTTGGCGAATATA+TGG + chr6.4:5939612-5939631 MS.gene65546:CDS 30.0%
TACAATCATTTACTGCAGAA+CGG - chr6.4:5940834-5940853 None:intergenic 30.0%
TGTTTGTCAGATGTCTATTA+AGG + chr6.4:5939366-5939385 MS.gene65546:CDS 30.0%
TTAATAGACATCTGACAAAC+AGG - chr6.4:5939367-5939386 None:intergenic 30.0%
TTCAGATTTGTATCTACCTT+TGG - chr6.4:5940213-5940232 None:intergenic 30.0%
! ACATCTGAATGTTGTTTACT+AGG - chr6.4:5940000-5940019 None:intergenic 30.0%
! ATACTTTTGAACCAGGTAAA+AGG - chr6.4:5940886-5940905 None:intergenic 30.0%
! ATCTTTTTAAATCGGACCAA+TGG - chr6.4:5939712-5939731 None:intergenic 30.0%
! CAAATAAGGTCTTTTAGACT+AGG - chr6.4:5940404-5940423 None:intergenic 30.0%
! CATCTGAATGTTGTTTACTA+GGG - chr6.4:5939999-5940018 None:intergenic 30.0%
! CCTTATTTGTGTCTATAAAC+CGG + chr6.4:5940415-5940434 MS.gene65546:intron 30.0%
! TACTTTACTGTTGATAGAGA+AGG + chr6.4:5940514-5940533 MS.gene65546:intron 30.0%
! TTTACCTGGTTCAAAAGTAT+TGG + chr6.4:5940886-5940905 MS.gene65546:intron 30.0%
!! AGAGAAGTTTGTTATTTGCT+TGG + chr6.4:5940481-5940500 MS.gene65546:intron 30.0%
!!! GGTCCTTTTGTTATTTTTGT+GGG + chr6.4:5939782-5939801 MS.gene65546:CDS 30.0%
AAAACGTCCAACTAGAACAT+AGG - chr6.4:5939284-5939303 None:intergenic 35.0%
AATTCAGTCTCGTTTACATC+CGG - chr6.4:5940126-5940145 None:intergenic 35.0%
ATCCGGAGTTAAATTTGACT+TGG - chr6.4:5940109-5940128 None:intergenic 35.0%
CAATTGTTTACCTGCAGTAA+TGG - chr6.4:5939590-5939609 None:intergenic 35.0%
CATTACTGCAGGTAAACAAT+TGG + chr6.4:5939588-5939607 MS.gene65546:CDS 35.0%
CCGGTTTATAGACACAAATA+AGG - chr6.4:5940418-5940437 None:intergenic 35.0%
CGTTTCAAATATGAGAGACT+CGG + chr6.4:5939760-5939779 MS.gene65546:CDS 35.0%
GTAAAAGGATATCCTTCTAG+AGG - chr6.4:5940871-5940890 None:intergenic 35.0%
GTTTCAAATATGAGAGACTC+GGG + chr6.4:5939761-5939780 MS.gene65546:CDS 35.0%
TATGAGTATTCGTGTTATGC+TGG + chr6.4:5939675-5939694 MS.gene65546:CDS 35.0%
TATGCTAATGCTACTGATGA+TGG + chr6.4:5939841-5939860 MS.gene65546:CDS 35.0%
TATTCGATTCTATGCGATGA+AGG + chr6.4:5939318-5939337 MS.gene65546:CDS 35.0%
TGTAAACGAGACTGAATTGA+TGG + chr6.4:5940128-5940147 MS.gene65546:CDS 35.0%
TTACCAGTGATGACAAACAA+GGG + chr6.4:5940773-5940792 MS.gene65546:intron 35.0%
TTGTCAGATGTCTATTAAGG+AGG + chr6.4:5939369-5939388 MS.gene65546:CDS 35.0%
! AGTTCCAATACTTTTGAACC+AGG - chr6.4:5940893-5940912 None:intergenic 35.0%
! TAGAAGGATATCCTTTTACC+TGG + chr6.4:5940872-5940891 MS.gene65546:intron 35.0%
! TGATTGAGAGGAACAGATTT+TGG - chr6.4:5939520-5939539 None:intergenic 35.0%
!! AAATTTGACTTGGTTGTCGA+GGG - chr6.4:5940099-5940118 None:intergenic 35.0%
!! TAAATTTGACTTGGTTGTCG+AGG - chr6.4:5940100-5940119 None:intergenic 35.0%
!! TGATCCATTGTAGTTTGTTG+TGG - chr6.4:5940282-5940301 None:intergenic 35.0%
!!! GGATCAGCAGCATTTTTTAT+CGG + chr6.4:5940296-5940315 MS.gene65546:intron 35.0%
!!! GGGTCCTTTTGTTATTTTTG+TGG + chr6.4:5939781-5939800 MS.gene65546:CDS 35.0%
!!! TGTTATTTTTGTGGGTTGCT+TGG + chr6.4:5939790-5939809 MS.gene65546:CDS 35.0%
AAACGAACTTCATGTGAACC+AGG - chr6.4:5939745-5939764 None:intergenic 40.0%
AAATACTCATTTCGTCCGAC+AGG - chr6.4:5940340-5940359 None:intergenic 40.0%
AAGCCATTATAAAGCCAAGC+CGG - chr6.4:5940811-5940830 None:intergenic 40.0%
ACGCTGCACATCTTTCATAA+TGG - chr6.4:5940253-5940272 None:intergenic 40.0%
ATAGAATCGAATACGACGGT+CGG - chr6.4:5939311-5939330 None:intergenic 40.0%
ATGGCGACTAACTTCTTTCT+GGG + chr6.4:5940645-5940664 MS.gene65546:intron 40.0%
CAAACAGGTTTCATGAACTC+CGG - chr6.4:5939352-5939371 None:intergenic 40.0%
CTAGAACATAGGAACCTTCA+AGG - chr6.4:5939273-5939292 None:intergenic 40.0%
CTCTCAATCATATACCCTTC+TGG + chr6.4:5939530-5939549 MS.gene65546:CDS 40.0%
CTTACCAGTGATGACAAACA+AGG + chr6.4:5940772-5940791 MS.gene65546:intron 40.0%
GAAAGAACTGGAATAGGTAC+AGG - chr6.4:5939405-5939424 None:intergenic 40.0%
GAAGGTTCCTATGTTCTAGT+TGG + chr6.4:5939274-5939293 MS.gene65546:intron 40.0%
GATAGGTGAATGATCTGAAG+TGG - chr6.4:5941010-5941029 None:intergenic 40.0%
GGTTCCACAACAAACTACAA+TGG + chr6.4:5940275-5940294 MS.gene65546:intron 40.0%
GTGGATGTTCTTGATCAAAG+TGG + chr6.4:5939914-5939933 MS.gene65546:intron 40.0%
TACCAGTGATGACAAACAAG+GGG + chr6.4:5940774-5940793 MS.gene65546:intron 40.0%
TATGAAAGATGTGCAGCGTA+CGG + chr6.4:5940254-5940273 MS.gene65546:intron 40.0%
TATGGCGACTAACTTCTTTC+TGG + chr6.4:5940644-5940663 MS.gene65546:intron 40.0%
TCCACTACCATTTCTCTTCA+CGG - chr6.4:5939899-5939918 None:intergenic 40.0%
TCGCATAGAATCGAATACGA+CGG - chr6.4:5939315-5939334 None:intergenic 40.0%
TGCTGCATAATTCATCTGTG+TGG - chr6.4:5939816-5939835 None:intergenic 40.0%
TTTAGACTAGGAACTGCCTT+AGG - chr6.4:5940392-5940411 None:intergenic 40.0%
! ATTTTATGTTGGAACTGCCG+AGG + chr6.4:5940356-5940375 MS.gene65546:intron 40.0%
! CAGAAGGGTATATGATTGAG+AGG - chr6.4:5939532-5939551 None:intergenic 40.0%
! GTACTAGGAATTTTGCAGCA+AGG + chr6.4:5939487-5939506 MS.gene65546:CDS 40.0%
!! GTACTGTTAAAAAGCGCTCA+AGG + chr6.4:5940166-5940185 MS.gene65546:CDS 40.0%
!!! CATTTTTTATCGGCAGAGTC+TGG + chr6.4:5940306-5940325 MS.gene65546:intron 40.0%
AAAGGTCCATGGTCTTTCGA+CGG + chr6.4:5939454-5939473 MS.gene65546:CDS 45.0%
ACATGTCCTGCTTTCTGTGA+TGG - chr6.4:5939976-5939995 None:intergenic 45.0%
ACCAGTGATGACAAACAAGG+GGG + chr6.4:5940775-5940794 MS.gene65546:intron 45.0%
ACTACCATTTCTCTTCACGG+GGG - chr6.4:5939896-5939915 None:intergenic 45.0%
ATCATGGACTTGCAACCAGA+AGG - chr6.4:5939548-5939567 None:intergenic 45.0%
ATGCACAACGTCGACAATCT+TGG + chr6.4:5940680-5940699 MS.gene65546:intron 45.0%
ATGGAGCGAGTACTCAAGAA+AGG + chr6.4:5939436-5939455 MS.gene65546:CDS 45.0%
CAAGTTAATGACCCAGACAG+AGG + chr6.4:5940619-5940638 MS.gene65546:intron 45.0%
CACTACCATTTCTCTTCACG+GGG - chr6.4:5939897-5939916 None:intergenic 45.0%
CCACTACCATTTCTCTTCAC+GGG - chr6.4:5939898-5939917 None:intergenic 45.0%
GAGGGTGAAAGAACTGGAAT+AGG - chr6.4:5939411-5939430 None:intergenic 45.0%
GAGTACTCAAGAAAGGTCCA+TGG + chr6.4:5939443-5939462 MS.gene65546:CDS 45.0%
GATGGTGCAACAATCCTAAC+CGG - chr6.4:5939958-5939977 None:intergenic 45.0%
GCTGGATGTTCGAAAACCAT+TGG + chr6.4:5939693-5939712 MS.gene65546:CDS 45.0%
TATAATACACCAAGGGTCCG+TGG - chr6.4:5940734-5940753 None:intergenic 45.0%
TCATGGACTTGCAACCAGAA+GGG - chr6.4:5939547-5939566 None:intergenic 45.0%
TGGACATAACAGCAAGGTCA+CGG - chr6.4:5940714-5940733 None:intergenic 45.0%
TGGCGACTAACTTCTTTCTG+GGG + chr6.4:5940646-5940665 MS.gene65546:intron 45.0%
TTACATGACCTCCCTCTAGA+AGG + chr6.4:5940856-5940875 MS.gene65546:intron 45.0%
TTCATCCTCTTCACCTGCAA+TGG - chr6.4:5939209-5939228 None:intergenic 45.0%
! AAGGATATCCTTCTAGAGGG+AGG - chr6.4:5940867-5940886 None:intergenic 45.0%
! ACGGACCCTTGGTGTATTAT+AGG + chr6.4:5940733-5940752 MS.gene65546:intron 45.0%
! GTTGATAGAGAAGGACGTAG+TGG + chr6.4:5940523-5940542 MS.gene65546:intron 45.0%
! TAATGGACATACAGCCGATC+GGG - chr6.4:5939573-5939592 None:intergenic 45.0%
!! AGACCGGCTTGGCTTTATAA+TGG + chr6.4:5940805-5940824 MS.gene65546:intron 45.0%
!! GTAATGGACATACAGCCGAT+CGG - chr6.4:5939574-5939593 None:intergenic 45.0%
!! GTGGTGGTCTTGCTGTTATT+TGG + chr6.4:5940542-5940561 MS.gene65546:intron 45.0%
!! TGACCTTGCTGTTATGTCCA+CGG + chr6.4:5940714-5940733 MS.gene65546:intron 45.0%
!! TGATTCTGGAACTGCACCAA+AGG + chr6.4:5940194-5940213 MS.gene65546:CDS 45.0%
!! AATAATTTATAATATTACAT+TGG - chr6.4:5940579-5940598 None:intergenic 5.0%
AAGGTCCATGGTCTTTCGAC+GGG + chr6.4:5939455-5939474 MS.gene65546:CDS 50.0%
ACATAACAGCAAGGTCACGG+AGG - chr6.4:5940711-5940730 None:intergenic 50.0%
ATAAAGCCAAGCCGGTCTCT+CGG - chr6.4:5940803-5940822 None:intergenic 50.0%
ATTTGTGAACGCCTCAGCCA+TGG - chr6.4:5940058-5940077 None:intergenic 50.0%
CAAGTCCATGATGTCCCGAT+CGG + chr6.4:5939556-5939575 MS.gene65546:CDS 50.0%
CACGTAGCTCAGAACTCCAA+AGG - chr6.4:5939873-5939892 None:intergenic 50.0%
CCAGTGATGACAAACAAGGG+GGG + chr6.4:5940776-5940795 MS.gene65546:intron 50.0%
CCCGTGAAGAGAAATGGTAG+TGG + chr6.4:5939895-5939914 MS.gene65546:intron 50.0%
CCTCTTCACCTGCAATGGAA+AGG - chr6.4:5939204-5939223 None:intergenic 50.0%
CCTTTCCATTGCAGGTGAAG+AGG + chr6.4:5939201-5939220 MS.gene65546:intron 50.0%
CGGGAAAAGAGTAAGCCATG+AGG - chr6.4:5940959-5940978 None:intergenic 50.0%
GAGGGAGGGAATAAAGTAGC+AGG - chr6.4:5940081-5940100 None:intergenic 50.0%
GAGTAAGCCATGAGGATGTG+TGG - chr6.4:5940951-5940970 None:intergenic 50.0%
GATAGAGAAGGACGTAGTGG+TGG + chr6.4:5940526-5940545 MS.gene65546:intron 50.0%
GGCTGTATGTCCATTACTGC+AGG + chr6.4:5939577-5939596 MS.gene65546:CDS 50.0%
GTTCTTTCACCCTCGTGACA+TGG + chr6.4:5939417-5939436 MS.gene65546:CDS 50.0%
GTTGCACCATCACAGAAAGC+AGG + chr6.4:5939967-5939986 MS.gene65546:intron 50.0%
TGCGATGAAGGAGAAGATGC+CGG + chr6.4:5939330-5939349 MS.gene65546:CDS 50.0%
TGTCACGAGGGTGAAAGAAC+TGG - chr6.4:5939417-5939436 None:intergenic 50.0%
TTGATCAAAGTGGCTGCGAG+AGG + chr6.4:5939924-5939943 MS.gene65546:intron 50.0%
! ATGACCCAGACAGAGGCATA+TGG + chr6.4:5940626-5940645 MS.gene65546:intron 50.0%
! ATGGAGCACCTTTCCATTGC+AGG + chr6.4:5939193-5939212 MS.gene65546:CDS 50.0%
!! AAGTATTGGAACTCCTCGCG+CGG + chr6.4:5940900-5940919 MS.gene65546:intron 50.0%
!! CGCTCAAGGAGTACTGATTC+TGG + chr6.4:5940180-5940199 MS.gene65546:CDS 50.0%
!! TTGACTTGGTTGTCGAGGGA+GGG - chr6.4:5940095-5940114 None:intergenic 50.0%
!! TTTGACTTGGTTGTCGAGGG+AGG - chr6.4:5940096-5940115 None:intergenic 50.0%
AGATGCCCGTCGAAAGACCA+TGG - chr6.4:5939463-5939482 None:intergenic 55.0%
AGTACTCGCTCCATGTCACG+AGG - chr6.4:5939430-5939449 None:intergenic 55.0%
AGTCGCCATATGCCTCTGTC+TGG - chr6.4:5940634-5940653 None:intergenic 55.0%
AGTCTGGTTACCAGGCCTGT+CGG + chr6.4:5940322-5940341 MS.gene65546:intron 55.0%
AGTCTTTCTTCAACCGCGCG+AGG - chr6.4:5940916-5940935 None:intergenic 55.0%
ATCGGCAGAGTCTGGTTACC+AGG + chr6.4:5940314-5940333 MS.gene65546:intron 55.0%
ATGTTGGAACTGCCGAGGTC+TGG + chr6.4:5940361-5940380 MS.gene65546:intron 55.0%
CCTTAGGATGTCCCAGACCT+CGG - chr6.4:5940376-5940395 None:intergenic 55.0%
CTCGAGGACGCATATCTCCA+TGG + chr6.4:5940038-5940057 MS.gene65546:CDS 55.0%
CTGTTATGTCCACGGACCCT+TGG + chr6.4:5940722-5940741 MS.gene65546:intron 55.0%
GACGCATATCTCCATGGCTG+AGG + chr6.4:5940044-5940063 MS.gene65546:CDS 55.0%
GACGGGCATCTTCTGGTACT+AGG + chr6.4:5939472-5939491 MS.gene65546:CDS 55.0%
GAGCGTTCCACACATCCTCA+TGG + chr6.4:5940941-5940960 MS.gene65546:intron 55.0%
GGAACCTTCAAGGCTCTCAG+CGG - chr6.4:5939263-5939282 None:intergenic 55.0%
GGTCCGTGGACATAACAGCA+AGG - chr6.4:5940720-5940739 None:intergenic 55.0%
GTACTCGCTCCATGTCACGA+GGG - chr6.4:5939429-5939448 None:intergenic 55.0%
GTCGCCATATGCCTCTGTCT+GGG - chr6.4:5940633-5940652 None:intergenic 55.0%
GTCTTTCGACGGGCATCTTC+TGG + chr6.4:5939465-5939484 MS.gene65546:CDS 55.0%
TACAGCCGATCGGGACATCA+TGG - chr6.4:5939564-5939583 None:intergenic 55.0%
TCAAAGTGGCTGCGAGAGGA+TGG + chr6.4:5939928-5939947 MS.gene65546:intron 55.0%
TGTTGGAACTGCCGAGGTCT+GGG + chr6.4:5940362-5940381 MS.gene65546:intron 55.0%
! CCCCCCTTGTTTGTCATCAC+TGG - chr6.4:5940779-5940798 None:intergenic 55.0%
!! CTGATGATGGCACACGCCTT+TGG + chr6.4:5939854-5939873 MS.gene65546:CDS 55.0%
AAGTGGCTGCGAGAGGATGG+TGG + chr6.4:5939931-5939950 MS.gene65546:intron 60.0%
CGTGCCCCCGTGAAGAGAAA+TGG + chr6.4:5939889-5939908 MS.gene65546:CDS 60.0%
CTCATTTCGTCCGACAGGCC+TGG - chr6.4:5940335-5940354 None:intergenic 60.0%
GTTGCCGCTGAGAGCCTTGA+AGG + chr6.4:5939256-5939275 MS.gene65546:intron 60.0%
! CCGAGGTCTGGGACATCCTA+AGG + chr6.4:5940373-5940392 MS.gene65546:intron 60.0%
! GAGAGGATGGTGGTCCGGTT+AGG + chr6.4:5939941-5939960 MS.gene65546:intron 60.0%
ACAAGGGGGGTCCGAGAGAC+CGG + chr6.4:5940789-5940808 MS.gene65546:intron 65.0%
! GCTGCGAGAGGATGGTGGTC+CGG + chr6.4:5939936-5939955 MS.gene65546:intron 65.0%
GGGGGTCCGAGAGACCGGCT+TGG + chr6.4:5940794-5940813 MS.gene65546:intron 75.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 5939193 5941036 5939193 ID=MS.gene65546
chr6.4 mRNA 5939193 5941036 5939193 ID=MS.gene65546.t1;Parent=MS.gene65546
chr6.4 exon 5939193 5939214 5939193 ID=MS.gene65546.t1.exon1;Parent=MS.gene65546.t1
chr6.4 CDS 5939193 5939214 5939193 ID=cds.MS.gene65546.t1;Parent=MS.gene65546.t1
chr6.4 exon 5939293 5939910 5939293 ID=MS.gene65546.t1.exon2;Parent=MS.gene65546.t1
chr6.4 CDS 5939293 5939910 5939293 ID=cds.MS.gene65546.t1;Parent=MS.gene65546.t1
chr6.4 exon 5940028 5940215 5940028 ID=MS.gene65546.t1.exon3;Parent=MS.gene65546.t1
chr6.4 CDS 5940028 5940215 5940028 ID=cds.MS.gene65546.t1;Parent=MS.gene65546.t1
chr6.4 exon 5941007 5941036 5941007 ID=MS.gene65546.t1.exon4;Parent=MS.gene65546.t1
chr6.4 CDS 5941007 5941036 5941007 ID=cds.MS.gene65546.t1;Parent=MS.gene65546.t1
Gene Sequence

>MS.gene65546

ATGGAGCACCTTTCCATTGCAGGTGAAGAGGATGAAATCGAACTTTAATTTGATGAACAGCAAGTTGCCGCTGAGAGCCTTGAAGGTTCCTATGTTCTAGTTGGACGTTTTCTCACCGACCGTCGTATTCGATTCTATGCGATGAAGGAGAAGATGCCGGAGTTCATGAAACCTGTTTGTCAGATGTCTATTAAGGAGGTTGATGACAACCTGTACCTATTCCAGTTCTTTCACCCTCGTGACATGGAGCGAGTACTCAAGAAAGGTCCATGGTCTTTCGACGGGCATCTTCTGGTACTAGGAATTTTGCAGCAAGGAAGCACGCCAAAATCTGTTCCTCTCAATCATATACCCTTCTGGTTGCAAGTCCATGATGTCCCGATCGGCTGTATGTCCATTACTGCAGGTAAACAATTGGGTAATTTCGTTGGCGAATATATGGAGTATGACGAGAAAAATAATTCCAATTTTCTTGCTTCTTATATGAGTATTCGTGTTATGCTGGATGTTCGAAAACCATTGGTCCGATTTAAAAAGATTAAGACTAAGCCTGGTTCACATGAAGTTCGTTTCAAATATGAGAGACTCGGGTCCTTTTGTTATTTTTGTGGGTTGCTTGGCCACACAGATGAATTATGCAGCAGCTTGTATGCTAATGCTACTGATGATGGCACACGCCTTTGGAGTTCTGAGCTACGTGCCCCCGTGAAGAGAAATGGTAGTGGATGTTCTTGATCAAAGTGGCTGCGAGAGGATGGTGGTCCGGTTAGGATTGTTGCACCATCACAGAAAGCAGGACATGTCCCTAGTAAACAACATTCAGATGTTCCAATAGTAATTAATAACTCGAGGACGCATATCTCCATGGCTGAGGCGTTCACAAATCCTGCTACTTTATTCCCTCCCTCGACAACCAAGTCAAATTTAACTCCGGATGTAAACGAGACTGAATTGATGGAAGAAGAATTAAATGGTACTGTTAAAAAGCGCTCAAGGAGTACTGATTCTGGAACTGCACCAAAGGTAGATACAAATCTGAATTAACAGTCTGACATGACCATTATGAAAGATGTGCAGCGTACGGTTCCACAACAAACTACAATGGATCAGCAGCATTTTTTATCGGCAGAGTCTGGTTACCAGGCCTGTCGGACGAAATGAGTATTTTATGTTGGAACTGCCGAGGTCTGGGACATCCTAAGGCAGTTCCTAGTCTAAAAGACCTTATTTGTGTCTATAAACCGGATATTTTTTCCTTATTGAAACTTTGTCTCACTCTAATAAAATAAGAGAAGTTTGTTATTTGCTTGGTTATGATTCTTACTTTACTGTTGATAGAGAAGGACGTAGTGGTGGTCTTGCTGTTATTTGGAAGTCCTCTATCCAATGTAATATTATAAATTATTCCTCCAATTTCATTAATTTGCAAGTTAATGACCCAGACAGAGGCATATGGCGACTAACTTCTTTCTGGGGCTATTCTAACAATGCACAACGTCGACAATCTTGGAATCTCCTCCGTGACCTTGCTGTTATGTCCACGGACCCTTGGTGTATTATAGGTGATTTTAATGATCTTCTTACCAGTGATGACAAACAAGGGGGGTCCGAGAGACCGGCTTGGCTTTATAATGGCTTCCGTTCTGCAGTAAATGATTGTAATTTACATGACCTCCCTCTAGAAGGATATCCTTTTACCTGGTTCAAAAGTATTGGAACTCCTCGCGCGGTTGAAGAAAGACTAGATAGAGCGTTCCACACATCCTCATGGCTTACTCTTTTCCCGAATGCAAGATTAATTAATCTTGTTGCAGCCACTTCAGATCATTCACCTATCCTTCTAA
Protein sequence

>MS.gene65546.t1

MEHLSIAVGRFLTDRRIRFYAMKEKMPEFMKPVCQMSIKEVDDNLYLFQFFHPRDMERVLKKGPWSFDGHLLVLGILQQGSTPKSVPLNHIPFWLQVHDVPIGCMSITAGKQLGNFVGEYMEYDEKNNSNFLASYMSIRVMLDVRKPLVRFKKIKTKPGSHEVRFKYERLGSFCYFCGLLGHTDELCSSLYANATDDGTRLWSSELRAPVKRNVINNSRTHISMAEAFTNPATLFPPSTTKSNLTPDVNETELMEEELNGTVKKRSRSTDSGTAPKPLQIIHLSF