AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021647


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021647.t1 MTR_0010s0370 48.187 193 95 2 1 193 1 188 9.11e-62 191
MS.gene021647.t1 MTR_0010s0350 48.187 193 95 2 1 193 1 188 9.11e-62 191
MS.gene021647.t1 MTR_1g036720 45.192 104 55 1 1 104 1 102 2.92e-28 103
MS.gene021647.t1 MTR_6g033215 31.944 144 98 0 34 177 127 270 1.35e-26 103
MS.gene021647.t1 MTR_7g089990 44.660 103 55 1 104 204 84 186 5.80e-21 87.0
MS.gene021647.t1 MTR_3g053920 29.412 136 79 2 35 170 35 153 6.56e-17 77.4
MS.gene021647.t1 MTR_8g468010 39.437 71 43 0 122 192 6 76 2.26e-15 70.5
MS.gene021647.t1 MTR_4g103530 34.409 93 61 0 55 147 1 93 8.47e-15 70.1
MS.gene021647.t1 MTR_2g061500 32.979 94 63 0 36 129 36 129 1.30e-14 68.2
MS.gene021647.t1 MTR_1g036710 44.262 61 34 0 134 194 1 61 2.76e-13 67.4
MS.gene021647.t1 MTR_1g032270 30.000 100 67 1 100 196 1 100 9.81e-13 63.5
MS.gene021647.t1 MTR_7g106180 22.297 148 108 2 36 180 26 169 3.79e-12 63.5
MS.gene021647.t1 MTR_3g024240 29.565 115 81 0 84 198 55 169 4.85e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTTCTTTGTTCCCTTT+TGG 0.102132 7.4:-89947558 MS.gene021647:CDS
TGCTATTACTATGGCCTCTT+AGG 0.227274 7.4:-89947296 None:intergenic
GATCAAAACCACTTTCTTCA+TGG 0.293555 7.4:+89947833 None:intergenic
TACTTTGGAAAACTTAAGTT+TGG 0.316088 7.4:-89947899 MS.gene021647:CDS
TCCTAACCAGTTTGTTTGTC+AGG 0.321531 7.4:+89947785 None:intergenic
TGATTACGTAGGGGAGCTTC+TGG 0.329299 7.4:-89947476 MS.gene021647:CDS
AGAAGTACATGGCGAGAATA+TGG 0.339448 7.4:-89947750 MS.gene021647:CDS
ATACATGAGAATTCGCATTA+TGG 0.360770 7.4:-89947416 MS.gene021647:CDS
AAAACTGTGGGTCAAAATAT+TGG 0.383555 7.4:-89947499 MS.gene021647:CDS
TGTCTAAATGATGAAAGAAT+TGG 0.384191 7.4:+89947674 None:intergenic
ATTCATTCACATGTCTACTT+TGG 0.386983 7.4:-89947914 None:intergenic
TCATGGAATATTTCTTCTGC+TGG 0.390862 7.4:+89947850 None:intergenic
CAACTCAATATGAAAAGCTT+GGG 0.420739 7.4:-89947325 MS.gene021647:CDS
TAACAAACATATGTTGGTCC+TGG 0.438011 7.4:-89947617 MS.gene021647:CDS
CCGTTTCCTGACAAACAAAC+TGG 0.439070 7.4:-89947791 MS.gene021647:CDS
GAAAGGAGACGATGCAAAAC+CGG 0.449687 7.4:-89947350 MS.gene021647:CDS
CCGTCGGTAAATTATGAACT+TGG 0.456400 7.4:+89947533 None:intergenic
CCAGTTTGTTTGTCAGGAAA+CGG 0.457268 7.4:+89947791 None:intergenic
TCAACTCAATATGAAAAGCT+TGG 0.458829 7.4:-89947326 MS.gene021647:CDS
CATCGTCTCCTTTCTTCTTG+AGG 0.469546 7.4:+89947359 None:intergenic
AGACATGCAAAAGGTATTGA+AGG 0.472922 7.4:-89947656 MS.gene021647:CDS
GTCCTGGGTGCGCTAGGAGA+TGG 0.477875 7.4:-89947601 MS.gene021647:CDS
TTTGGAAAACTTAAGTTTGG+AGG 0.480432 7.4:-89947896 MS.gene021647:CDS
GAAATATTCCATGAAGAAAG+TGG 0.481705 7.4:-89947841 MS.gene021647:CDS
TCCTGACAAACAAACTGGTT+AGG 0.490300 7.4:-89947786 MS.gene021647:CDS
AGTCATCTATGTGGCCTAAG+AGG 0.499334 7.4:+89947282 None:intergenic
CTAATAGTCATTTGTCTCAT+CGG 0.500651 7.4:+89947724 None:intergenic
GATCCAGCTCTATGTTTGGT+AGG 0.502786 7.4:-89947814 MS.gene021647:CDS
ACCCAAAAGGGAACAAAGAA+AGG 0.505582 7.4:+89947556 None:intergenic
AAAATATTGGTGATTACGTA+GGG 0.505829 7.4:-89947486 MS.gene021647:CDS
TTATGAACTTGGACCCAAAA+GGG 0.512511 7.4:+89947544 None:intergenic
ATGTTGGTCCTGGGTGCGCT+AGG 0.516437 7.4:-89947607 MS.gene021647:CDS
CAAGTTCATAATTTACCGAC+GGG 0.518844 7.4:-89947532 MS.gene021647:CDS
AACAAACATATGTTGGTCCT+GGG 0.519336 7.4:-89947616 MS.gene021647:CDS
ATTATGAACTTGGACCCAAA+AGG 0.521467 7.4:+89947543 None:intergenic
TCATCATTTAGACATGCAAA+AGG 0.526826 7.4:-89947665 MS.gene021647:CDS
GGTGGACGTTCGAAAGCCTC+TGG 0.528850 7.4:-89947395 MS.gene021647:CDS
GACATGCAAAAGGTATTGAA+GGG 0.536397 7.4:-89947655 MS.gene021647:CDS
GTTCTTTAACAAACATATGT+TGG 0.540557 7.4:-89947623 MS.gene021647:CDS
ACTATTAGAGAAGCTAGTCC+AGG 0.551145 7.4:-89947709 MS.gene021647:CDS
TCTAAGCGCCTCAAGAAGAA+AGG 0.554659 7.4:-89947367 MS.gene021647:CDS
CGGCCTACCAAACATAGAGC+TGG 0.554828 7.4:+89947811 None:intergenic
CAAAATATTGGTGATTACGT+AGG 0.565021 7.4:-89947487 MS.gene021647:CDS
CCAAGTTCATAATTTACCGA+CGG 0.588123 7.4:-89947533 MS.gene021647:CDS
CTCCATCTCCTAGCGCACCC+AGG 0.597175 7.4:+89947599 None:intergenic
CATGAGAATTCGCATTATGG+TGG 0.610643 7.4:-89947413 MS.gene021647:CDS
AGGCGCTTAGATTTCACCAG+AGG 0.612441 7.4:+89947379 None:intergenic
CAAAAGGGAACAAAGAAAGG+TGG 0.614481 7.4:+89947559 None:intergenic
ATATGTTTGTTAAAGAACCA+CGG 0.642717 7.4:+89947628 None:intergenic
AAAATAGTTCGAACTTCTGG+AGG 0.646242 7.4:-89947441 MS.gene021647:CDS
CATGCAAAAGGTATTGAAGG+GGG 0.657037 7.4:-89947653 MS.gene021647:CDS
ACATGCAAAAGGTATTGAAG+GGG 0.659842 7.4:-89947654 MS.gene021647:CDS
ATGCAAAAGGTATTGAAGGG+GGG 0.673990 7.4:-89947652 MS.gene021647:CDS
TGATGTGATGAAGAAGTACA+TGG 0.674954 7.4:-89947761 MS.gene021647:CDS
AAATATTGGTGATTACGTAG+GGG 0.720480 7.4:-89947485 MS.gene021647:CDS
AAGTTCATAATTTACCGACG+GGG 0.750832 7.4:-89947531 MS.gene021647:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAAAATAGTTCGAACTTC+TGG - chr7.4:89947757-89947776 MS.gene021647:CDS 25.0%
! GTTCTTTAACAAACATATGT+TGG - chr7.4:89947578-89947597 MS.gene021647:CDS 25.0%
! TGTCTAAATGATGAAAGAAT+TGG + chr7.4:89947530-89947549 None:intergenic 25.0%
!! AAAATATTGGTGATTACGTA+GGG - chr7.4:89947715-89947734 MS.gene021647:CDS 25.0%
!! ATATGTTTGTTAAAGAACCA+CGG + chr7.4:89947576-89947595 None:intergenic 25.0%
!! TACTTTGGAAAACTTAAGTT+TGG - chr7.4:89947302-89947321 MS.gene021647:CDS 25.0%
AAAACTGTGGGTCAAAATAT+TGG - chr7.4:89947702-89947721 MS.gene021647:CDS 30.0%
AATTGGAAAACAAAAATGCC+TGG + chr7.4:89947513-89947532 None:intergenic 30.0%
ATACATGAGAATTCGCATTA+TGG - chr7.4:89947785-89947804 MS.gene021647:CDS 30.0%
CAACTCAATATGAAAAGCTT+GGG - chr7.4:89947876-89947895 MS.gene021647:CDS 30.0%
CTAATAGTCATTTGTCTCAT+CGG + chr7.4:89947480-89947499 None:intergenic 30.0%
GAAATATTCCATGAAGAAAG+TGG - chr7.4:89947360-89947379 MS.gene021647:CDS 30.0%
TCAACTCAATATGAAAAGCT+TGG - chr7.4:89947875-89947894 MS.gene021647:CDS 30.0%
TCATCATTTAGACATGCAAA+AGG - chr7.4:89947536-89947555 MS.gene021647:CDS 30.0%
TTTGGAAAACTTAAGTTTGG+AGG - chr7.4:89947305-89947324 MS.gene021647:CDS 30.0%
! AAATATTGGTGATTACGTAG+GGG - chr7.4:89947716-89947735 MS.gene021647:CDS 30.0%
! CAAAATATTGGTGATTACGT+AGG - chr7.4:89947714-89947733 MS.gene021647:CDS 30.0%
!!! CTTTTCATATTGAGTTGAAC+CGG + chr7.4:89947873-89947892 None:intergenic 30.0%
AAAATAGTTCGAACTTCTGG+AGG - chr7.4:89947760-89947779 MS.gene021647:CDS 35.0%
AACAAACATATGTTGGTCCT+GGG - chr7.4:89947585-89947604 MS.gene021647:CDS 35.0%
AAGTTCATAATTTACCGACG+GGG - chr7.4:89947670-89947689 MS.gene021647:CDS 35.0%
ATTATGAACTTGGACCCAAA+AGG + chr7.4:89947661-89947680 None:intergenic 35.0%
CAAGTTCATAATTTACCGAC+GGG - chr7.4:89947669-89947688 MS.gene021647:CDS 35.0%
CCAAGTTCATAATTTACCGA+CGG - chr7.4:89947668-89947687 MS.gene021647:CDS 35.0%
GATCAAAACCACTTTCTTCA+TGG + chr7.4:89947371-89947390 None:intergenic 35.0%
TAACAAACATATGTTGGTCC+TGG - chr7.4:89947584-89947603 MS.gene021647:CDS 35.0%
TCATGGAATATTTCTTCTGC+TGG + chr7.4:89947354-89947373 None:intergenic 35.0%
TTATGAACTTGGACCCAAAA+GGG + chr7.4:89947660-89947679 None:intergenic 35.0%
! ACCTTTCTTTGTTCCCTTTT+GGG - chr7.4:89947644-89947663 MS.gene021647:CDS 35.0%
! GGGATTTTCTGCTATTACTA+TGG - chr7.4:89947896-89947915 MS.gene021647:CDS 35.0%
! TGATGTGATGAAGAAGTACA+TGG - chr7.4:89947440-89947459 MS.gene021647:CDS 35.0%
! TTTTGATCCAGCTCTATGTT+TGG - chr7.4:89947383-89947402 MS.gene021647:CDS 35.0%
!! ACATGCAAAAGGTATTGAAG+GGG - chr7.4:89947547-89947566 MS.gene021647:CDS 35.0%
!! AGACATGCAAAAGGTATTGA+AGG - chr7.4:89947545-89947564 MS.gene021647:CDS 35.0%
!! GACATGCAAAAGGTATTGAA+GGG - chr7.4:89947546-89947565 MS.gene021647:CDS 35.0%
ACCCAAAAGGGAACAAAGAA+AGG + chr7.4:89947648-89947667 None:intergenic 40.0%
ACTATTAGAGAAGCTAGTCC+AGG - chr7.4:89947492-89947511 MS.gene021647:CDS 40.0%
CAAAAGGGAACAAAGAAAGG+TGG + chr7.4:89947645-89947664 None:intergenic 40.0%
CATGAGAATTCGCATTATGG+TGG - chr7.4:89947788-89947807 MS.gene021647:CDS 40.0%
CCGTCGGTAAATTATGAACT+TGG + chr7.4:89947671-89947690 None:intergenic 40.0%
GGGTACATGTCAAAAACTGT+GGG - chr7.4:89947690-89947709 MS.gene021647:CDS 40.0%
TCCTGACAAACAAACTGGTT+AGG - chr7.4:89947415-89947434 MS.gene021647:CDS 40.0%
! AGAAGTACATGGCGAGAATA+TGG - chr7.4:89947451-89947470 MS.gene021647:CDS 40.0%
! CACCTTTCTTTGTTCCCTTT+TGG - chr7.4:89947643-89947662 MS.gene021647:CDS 40.0%
! CCAGTTTGTTTGTCAGGAAA+CGG + chr7.4:89947413-89947432 None:intergenic 40.0%
! TCCTAACCAGTTTGTTTGTC+AGG + chr7.4:89947419-89947438 None:intergenic 40.0%
!! ATGCAAAAGGTATTGAAGGG+GGG - chr7.4:89947549-89947568 MS.gene021647:CDS 40.0%
!! CATGCAAAAGGTATTGAAGG+GGG - chr7.4:89947548-89947567 MS.gene021647:CDS 40.0%
CATCGTCTCCTTTCTTCTTG+AGG + chr7.4:89947845-89947864 None:intergenic 45.0%
CCGTTTCCTGACAAACAAAC+TGG - chr7.4:89947410-89947429 MS.gene021647:CDS 45.0%
GAAAGGAGACGATGCAAAAC+CGG - chr7.4:89947851-89947870 MS.gene021647:CDS 45.0%
GATCCAGCTCTATGTTTGGT+AGG - chr7.4:89947387-89947406 MS.gene021647:CDS 45.0%
GGGGTACATGTCAAAAACTG+TGG - chr7.4:89947689-89947708 MS.gene021647:CDS 45.0%
TCTAAGCGCCTCAAGAAGAA+AGG - chr7.4:89947834-89947853 MS.gene021647:CDS 45.0%
! GTTTTTGACATGTACCCCGT+CGG + chr7.4:89947687-89947706 None:intergenic 45.0%
AGGCGCTTAGATTTCACCAG+AGG + chr7.4:89947825-89947844 None:intergenic 50.0%
TGATTACGTAGGGGAGCTTC+TGG - chr7.4:89947725-89947744 MS.gene021647:CDS 50.0%
CGGCCTACCAAACATAGAGC+TGG + chr7.4:89947393-89947412 None:intergenic 55.0%
ATGTTGGTCCTGGGTGCGCT+AGG - chr7.4:89947594-89947613 MS.gene021647:CDS 60.0%
GGTGGACGTTCGAAAGCCTC+TGG - chr7.4:89947806-89947825 MS.gene021647:CDS 60.0%
!! AGGTATTGAAGGGGGGTCCG+TGG - chr7.4:89947556-89947575 MS.gene021647:CDS 60.0%
CTCCATCTCCTAGCGCACCC+AGG + chr7.4:89947605-89947624 None:intergenic 65.0%
GTCCTGGGTGCGCTAGGAGA+TGG - chr7.4:89947600-89947619 MS.gene021647:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 89947297 89947926 89947297 ID=MS.gene021647
chr7.4 mRNA 89947297 89947926 89947297 ID=MS.gene021647.t1;Parent=MS.gene021647
chr7.4 exon 89947297 89947926 89947297 ID=MS.gene021647.t1.exon1;Parent=MS.gene021647.t1
chr7.4 CDS 89947297 89947926 89947297 ID=cds.MS.gene021647.t1;Parent=MS.gene021647.t1
Gene Sequence

>MS.gene021647

ATGTCTACTTTGGAAAACTTAAGTTTGGAGGAAGAAGAAGATGAGTTTGAAATTCCAGCAGAAGAAATATTCCATGAAGAAAGTGGTTTTGATCCAGCTCTATGTTTGGTAGGCCGTTTCCTGACAAACAAACTGGTTAGGACTGATGTGATGAAGAAGTACATGGCGAGAATATGGACTCCGATGAGACAAATGACTATTAGAGAAGCTAGTCCAGGCATTTTTGTTTTCCAATTCTTTCATCATTTAGACATGCAAAAGGTATTGAAGGGGGGTCCGTGGTTCTTTAACAAACATATGTTGGTCCTGGGTGCGCTAGGAGATGGAGAAGAACTAGAACAAGTTCCACCTTTCTTTGTTCCCTTTTGGGTCCAAGTTCATAATTTACCGACGGGGTACATGTCAAAAACTGTGGGTCAAAATATTGGTGATTACGTAGGGGAGCTTCTGGAGTATGATGAAAAAAATAGTTCGAACTTCTGGAGGAAATACATGAGAATTCGCATTATGGTGGACGTTCGAAAGCCTCTGGTGAAATCTAAGCGCCTCAAGAAGAAAGGAGACGATGCAAAACCGGTTCAACTCAATATGAAAAGCTTGGGATTTTCTGCTATTACTATGGCCTCTTAG

Protein sequence

>MS.gene021647.t1

MSTLENLSLEEEEDEFEIPAEEIFHEESGFDPALCLVGRFLTNKLVRTDVMKKYMARIWTPMRQMTIREASPGIFVFQFFHHLDMQKVLKGGPWFFNKHMLVLGALGDGEELEQVPPFFVPFWVQVHNLPTGYMSKTVGQNIGDYVGELLEYDEKNSSNFWRKYMRIRIMVDVRKPLVKSKRLKKKGDDAKPVQLNMKSLGFSAITMAS