AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86868

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86868.t1 MTR_6g055230 72.436 312 38 2 776 1054 1 297 8.39e-135 409
MS.gene86868.t1 MTR_6g033215 67.347 196 62 1 2 197 102 295 8.52e-91 292
MS.gene86868.t1 MTR_4g046707 47.917 288 93 5 821 1054 69 353 2.22e-72 244
MS.gene86868.t1 MTR_6g053790 43.431 274 89 6 133 403 35 245 6.29e-55 194
MS.gene86868.t1 MTR_8g468010 76.562 64 15 0 112 175 1 64 3.07e-29 114
MS.gene86868.t1 MTR_1g032270 68.056 72 23 0 95 166 1 72 7.04e-27 107
MS.gene86868.t1 MTR_4g046663 65.789 76 23 1 963 1038 4 76 7.75e-27 105
MS.gene86868.t1 MTR_0010s0370 34.615 156 100 1 19 174 24 177 8.11e-26 106
MS.gene86868.t1 MTR_0010s0350 34.615 156 100 1 19 174 24 177 8.11e-26 106
MS.gene86868.t1 MTR_6g053230 31.472 197 94 3 1 194 22 180 3.36e-24 108
MS.gene86868.t1 MTR_4g075740 41.333 150 72 6 2 149 22 157 3.36e-21 92.0
MS.gene86868.t1 MTR_3g024240 35.833 120 75 1 79 196 55 174 4.28e-19 86.7
MS.gene86868.t1 MTR_3g053920 28.994 169 101 3 1 169 8 157 2.62e-18 87.0
MS.gene86868.t1 MTR_4g020545 27.751 209 98 5 64 271 85 241 6.85e-17 83.2
MS.gene86868.t1 MTR_2g061500 30.303 132 88 2 4 135 11 138 9.58e-16 75.5
MS.gene86868.t1 MTR_3g041640 73.810 42 11 0 112 153 1 42 2.34e-15 74.7
MS.gene86868.t1 MTR_6g034215 44.186 86 45 1 653 738 209 291 4.70e-15 78.2
MS.gene86868.t1 MTR_1g100687 40.964 83 47 1 663 743 150 232 4.09e-14 73.6
MS.gene86868.t1 MTR_4g020630 44.186 86 45 1 653 738 141 223 1.75e-13 74.7
MS.gene86868.t1 MTR_8g463110 41.176 85 47 1 654 738 206 287 2.02e-13 75.1
MS.gene86868.t1 MTR_4g103530 40.000 75 45 0 68 142 19 93 5.96e-12 66.2
MS.gene86868.t1 MTR_7g011710 37.349 83 50 1 52 134 5 85 1.32e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86868.t1 AT1G05270 40.345 290 116 6 818 1053 79 365 2.95e-56 199
MS.gene86868.t1 AT2G32340 37.671 292 124 6 818 1054 65 353 1.22e-48 177
MS.gene86868.t1 AT2G32340 35.747 221 87 5 818 983 65 285 1.23e-31 125
MS.gene86868.t1 AT2G01050 23.243 185 134 3 28 212 80 256 1.85e-11 68.6

Find 295 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTCCAGATCACCTTTA+TGG 0.139058 6.2:-53131414 None:intergenic
CAATGAACTCAACAGAAATT+TGG 0.148827 6.2:+53131521 MS.gene86868:CDS
CGACGCCTTTGTTCTGTTTC+CGG 0.186757 6.2:-53138525 None:intergenic
ATGATAGATGGTTGATATTA+TGG 0.192301 6.2:-53142452 None:intergenic
TGTTTCATTTGTGGTTTAAT+TGG 0.212164 6.2:+53131784 MS.gene86868:CDS
TACCTTGTTGGCACCCTTCC+TGG 0.246051 6.2:+53138630 MS.gene86868:CDS
CCGTTGGAAGATTGTCAAAA+AGG 0.246922 6.2:-53140474 None:intergenic
TTCTTTAGCAATATCAGTTC+TGG 0.248653 6.2:-53133066 None:intergenic
AAGTCCATGCATAGCATTTA+TGG 0.256662 6.2:+53132033 MS.gene86868:CDS
TTGATTCAATCGATGATAAA+TGG 0.266459 6.2:-53131306 None:intergenic
CAAATTTCTGTTGAGTTCAT+TGG 0.276354 6.2:-53131520 None:intergenic
CTTTCAAGTTTGAGAATGAT+TGG 0.294431 6.2:+53133950 MS.gene86868:CDS
TTATTTGCATGCTGAAAATA+AGG 0.307002 6.2:+53131876 MS.gene86868:CDS
ACCAATACTGACTGCAATAA+AGG 0.311958 6.2:-53132630 None:intergenic
CCAACTGTCTTGTGACTCTA+TGG 0.312516 6.2:+53138584 MS.gene86868:CDS
AGTCATTCAAATTCAAATAA+TGG 0.314932 6.2:+53132003 MS.gene86868:CDS
TTATCAGATCCAGGAGCTTC+AGG 0.320349 6.2:+53142345 MS.gene86868:intron
CCAACCAGCCATTTGCTTGC+CGG 0.320998 6.2:-53131916 None:intergenic
TGGCACCCTTCCTGGCAATA+AGG 0.321229 6.2:+53138638 MS.gene86868:CDS
CATCGCAGGGATAGCCGGTT+TGG 0.322914 6.2:-53142389 None:intergenic
CTCCCACCATTGAACTTTGT+GGG 0.325742 6.2:-53132377 None:intergenic
TTGTTATTCTCTGTTGGAAT+TGG 0.331401 6.2:-53131373 None:intergenic
AGACAAATTCGGCTGGCTAT+TGG 0.335801 6.2:-53131227 None:intergenic
TGTGCAGCCTGGTTCTTTCA+TGG 0.336417 6.2:+53132974 MS.gene86868:CDS
GAGTTCATTGGAACGGCAAT+AGG 0.336558 6.2:-53131508 None:intergenic
TCAGCTCCCATTCGGCTTCA+TGG 0.341575 6.2:+53131555 MS.gene86868:CDS
CAAATGGCTGGTTGGTGGTC+GGG 0.342108 6.2:+53131924 MS.gene86868:CDS
TAGAAGGAATGAAGAAGAAT+TGG 0.344706 6.2:+53142079 MS.gene86868:CDS
TGTTAAAGTTGATATTAGTT+TGG 0.350730 6.2:+53132059 MS.gene86868:CDS
ATTGTTGGTATTGTTGTGGC+TGG 0.352966 6.2:+53142424 MS.gene86868:CDS
TGATCCGACTTTGTAAGGTT+TGG 0.356090 6.2:-53132234 None:intergenic
AATGAACTCAACAGAAATTT+GGG 0.358565 6.2:+53131522 MS.gene86868:CDS
TTTGTTCTGTTTCCGGTGAC+TGG 0.359494 6.2:-53138518 None:intergenic
TATAAAGAAGCAATCAAATA+TGG 0.360364 6.2:+53139687 MS.gene86868:CDS
TCTTTGCATTTAATGATAGA+TGG 0.369373 6.2:-53142464 None:intergenic
TAATTTGATCCGACTTTGTA+AGG 0.374662 6.2:-53132239 None:intergenic
ACCCCATTTCTTTCCACTGC+TGG 0.375779 6.2:-53131851 None:intergenic
TCTCCCACCATTGAACTTTG+TGG 0.376868 6.2:-53132378 None:intergenic
CAAGCAGAGCCAAGCCAGTC+AGG 0.379346 6.2:+53132883 MS.gene86868:CDS
CAACAGAACTGGTGGGCAGC+AGG 0.387159 6.2:+53133013 MS.gene86868:CDS
ATTAATCAAGCGTTGGTAGA+AGG 0.387488 6.2:+53132258 MS.gene86868:CDS
GCAAGGGTCGGAGCGCTGGT+TGG 0.392212 6.2:+53131580 MS.gene86868:CDS
CTTCCAGCAGTGGAAAGAAA+TGG 0.395183 6.2:+53131848 MS.gene86868:CDS
GATCACAAGGTCCTGCTTCA+AGG 0.395784 6.2:-53131701 None:intergenic
ATCGCAGGGATAGCCGGTTT+GGG 0.396142 6.2:-53142388 None:intergenic
AACTTGCGCTGCTTGTTGTT+TGG 0.399001 6.2:-53132933 None:intergenic
TGCAGGCAGTCCGTGGGTAC+TGG 0.399312 6.2:+53131441 MS.gene86868:CDS
ATCAAGATTAGGTCCACTTT+GGG 0.400905 6.2:+53131324 MS.gene86868:CDS
CTGTTTCCGGTGACTGGTCT+CGG 0.401105 6.2:-53138512 None:intergenic
ATGAGACTCTTTGCAATTGT+TGG 0.401820 6.2:+53142409 MS.gene86868:CDS
CTGACTGGCTTGGCTCTGCT+TGG 0.402827 6.2:-53132882 None:intergenic
TCATCGATTGAATCAAGATT+AGG 0.405459 6.2:+53131313 MS.gene86868:CDS
CAAGGGTCGGAGCGCTGGTT+GGG 0.410908 6.2:+53131581 MS.gene86868:CDS
CCCCTCCTCTCGTCTGCTGC+CGG 0.411788 6.2:-53131956 None:intergenic
GGTGGTCGGGGAAAGAATTC+CGG 0.412782 6.2:+53131937 MS.gene86868:CDS
CTGCTGCCCACCAGTTCTGT+TGG 0.413108 6.2:-53133012 None:intergenic
AGAACAGTTGTCGCTGTTGT+TGG 0.414234 6.2:+53142045 MS.gene86868:CDS
CCAAATAGTTGACGAAAATC+AGG 0.416747 6.2:-53132678 None:intergenic
TCTTGTTAGCCCTGGTGCCA+TGG 0.418022 6.2:+53136305 MS.gene86868:CDS
TCTTTAGCAATATCAGTTCT+GGG 0.418849 6.2:-53133065 None:intergenic
GCACGTGGGGCCAACAGAAC+TGG 0.420212 6.2:+53133002 MS.gene86868:CDS
GCAATTGTGCTCAGATAGTC+AGG 0.422961 6.2:+53139243 MS.gene86868:CDS
AATTAATTCCATACGTCTCC+AGG 0.423064 6.2:-53131350 None:intergenic
GGATGCAAGGGTCGGAGCGC+TGG 0.426344 6.2:+53131576 MS.gene86868:CDS
TTCCAGCAGTGGAAAGAAAT+GGG 0.427272 6.2:+53131849 MS.gene86868:CDS
AGCTTTGATTAAGCATCTTC+AGG 0.431617 6.2:+53138374 MS.gene86868:intron
TTTATCCTTCCTGACTGGCT+TGG 0.433405 6.2:-53132892 None:intergenic
GCCTTTATTGCAGTCAGTAT+TGG 0.435680 6.2:+53132629 MS.gene86868:CDS
AATCAAGATTAGGTCCACTT+TGG 0.436245 6.2:+53131323 MS.gene86868:CDS
TACAATTCCACCTCCCAAAC+CGG 0.438522 6.2:+53142375 MS.gene86868:CDS
CAGCAGGAGGAGCTCAAAGA+AGG 0.442557 6.2:+53133029 MS.gene86868:CDS
ATGAGGTTGTTAAAGATAGT+TGG 0.443035 6.2:+53133992 MS.gene86868:CDS
GGGATAGCCGGTTTGGGAGG+TGG 0.443122 6.2:-53142382 None:intergenic
AGCTCAGCCTGAGACAAATT+CGG 0.447734 6.2:-53131238 None:intergenic
TTGACAATCTTCCAACGGTA+AGG 0.448743 6.2:+53140479 MS.gene86868:CDS
TAGGTCCACTTTGGGATCCC+TGG 0.450840 6.2:+53131332 MS.gene86868:CDS
GGCCGTCGGCGATGACAAAC+CGG 0.451064 6.2:+53131897 MS.gene86868:CDS
GATCACCTTTATGGAAAAGT+TGG 0.451141 6.2:-53131405 None:intergenic
ATGAAAGAACCAGGCTGCAC+AGG 0.454751 6.2:-53132972 None:intergenic
CTCCCACGTGAGCAAAGAAA+TGG 0.454802 6.2:+53132507 MS.gene86868:CDS
GCCGAACGAAACGCAGCAAA+AGG 0.456746 6.2:-53139663 None:intergenic
AAATAAGCTGAGAAAGTGCT+TGG 0.457850 6.2:-53140448 None:intergenic
TCAAATTCAAATAATGGTTG+TGG 0.458318 6.2:+53132009 MS.gene86868:CDS
GATCTGGACAGAATTCTTGC+AGG 0.458812 6.2:+53131424 MS.gene86868:CDS
AATAGAAGAAGGAGTGAATG+AGG 0.460358 6.2:+53133975 MS.gene86868:CDS
TTGTTGGTATTGTTGTGGCT+GGG 0.463497 6.2:+53142425 MS.gene86868:CDS
TTTGTCTCAGGCTGAGCTGT+TGG 0.463582 6.2:+53131243 MS.gene86868:CDS
GCAGGGTGGTGAATGTTGTT+TGG 0.464546 6.2:-53138458 None:intergenic
AAATGGCTGGTTGGTGGTCG+GGG 0.465174 6.2:+53131925 MS.gene86868:CDS
AGTATCCCTCTTGTTAGCCC+TGG 0.465450 6.2:+53136297 MS.gene86868:CDS
TCGGCAGAAGGCATCGGAGG+TGG 0.467593 6.2:-53138493 None:intergenic
AAATTATTCTAACCTCTAAA+CGG 0.468316 6.2:-53139734 None:intergenic
TGCGCTGCTTGTTGTTTGGT+TGG 0.468435 6.2:-53132929 None:intergenic
ACTTGGTGATCGCCGTTTAG+AGG 0.468471 6.2:+53139722 MS.gene86868:CDS
GGTGCTTTATCCTTCCTGAC+TGG 0.469311 6.2:-53132897 None:intergenic
CCCTCCTTCCCTTGTACATG+TGG 0.470117 6.2:-53132474 None:intergenic
ATGACAAACCGGCAAGCAAA+TGG 0.470426 6.2:+53131908 MS.gene86868:CDS
TTCTTACGTACCTTATTGCC+AGG 0.471900 6.2:-53138648 None:intergenic
GTTTGCGATGTCTACCTTGT+TGG 0.476129 6.2:+53138618 MS.gene86868:CDS
TCATTAGTGTTAACTTCTGT+AGG 0.477335 6.2:-53132168 None:intergenic
TATGATGAGGAAAATAACTA+TGG 0.478297 6.2:+53131628 MS.gene86868:CDS
ATGCGGGTCAGAGTGGAGAT+TGG 0.479271 6.2:+53131667 MS.gene86868:CDS
AATGATTGGAGAATAGAAGA+AGG 0.479394 6.2:+53133964 MS.gene86868:CDS
TGCGGGTCAGAGTGGAGATT+GGG 0.479517 6.2:+53131668 MS.gene86868:CDS
TACGGAGCTCCTGAAGCTCC+TGG 0.479775 6.2:-53142354 None:intergenic
ACAACAATTAATGTTAATCA+AGG 0.480011 6.2:+53131979 MS.gene86868:CDS
CATGGAGTAAGCTGTCACTT+TGG 0.480245 6.2:+53140395 MS.gene86868:CDS
GTGTTCAAGTATGAACATCT+TGG 0.481535 6.2:+53131754 MS.gene86868:CDS
TGCCGGTTTGTCATCGCCGA+CGG 0.482839 6.2:-53131899 None:intergenic
CCTCCATTTCTTTGCTCACG+TGG 0.482854 6.2:-53132510 None:intergenic
AATGAAGTCACGTGGAAAGA+AGG 0.484674 6.2:+53132429 MS.gene86868:CDS
CAAGTTCATCAGCTCCCATT+CGG 0.485666 6.2:+53131547 MS.gene86868:CDS
CGGTGACTGGTCTCGGCAGA+AGG 0.486439 6.2:-53138505 None:intergenic
GATTCTTGTTTGAACATGGC+GGG 0.487258 6.2:+53132298 MS.gene86868:CDS
GCAAATGGCTGGTTGGTGGT+CGG 0.487611 6.2:+53131923 MS.gene86868:CDS
CTCCATTTCTTTGCTCACGT+GGG 0.490973 6.2:-53132509 None:intergenic
CTGACGGAGCAGGTTTAGTA+AGG 0.494150 6.2:+53132127 MS.gene86868:CDS
TTCTGTTGAGTTCATTGGAA+CGG 0.497309 6.2:-53131515 None:intergenic
TTTGAGCACGAGGTTGAGAA+AGG 0.499602 6.2:+53138431 MS.gene86868:CDS
TGTGTCGAAAGGAGTTGAAC+CGG 0.500294 6.2:+53134121 MS.gene86868:CDS
GTTGGAGAGAGGCATCTAGA+AGG 0.500498 6.2:+53142063 MS.gene86868:CDS
TTGCATCCATGAAGCCGAAT+GGG 0.502549 6.2:-53131561 None:intergenic
GATAGTTGGCAGAGTAATAG+TGG 0.504209 6.2:+53134006 MS.gene86868:CDS
TGCAATTGTTGGTATTGTTG+TGG 0.509689 6.2:+53142420 MS.gene86868:CDS
AGTCTCATCGCAGGGATAGC+CGG 0.512655 6.2:-53142394 None:intergenic
TTGTTTGGTTGGAAGTGAGT+TGG 0.514264 6.2:-53132918 None:intergenic
TGCTCCAAACCTTACAAAGT+CGG 0.516036 6.2:+53132230 MS.gene86868:CDS
ATTGCTAAAGAAGGTGATCA+AGG 0.516339 6.2:+53133077 MS.gene86868:CDS
TCTTTCATGGCTAGTGCACG+TGG 0.516739 6.2:+53132987 MS.gene86868:CDS
TTCCGGCAGCAGACGAGAGG+AGG 0.518935 6.2:+53131954 MS.gene86868:CDS
CCATAGAGTCACAAGACAGT+TGG 0.521024 6.2:-53138584 None:intergenic
GCACGCAACCCTGTGCAGCC+TGG 0.521211 6.2:+53132963 MS.gene86868:CDS
CACCTTCTCTTTCGATCACA+AGG 0.521365 6.2:-53131714 None:intergenic
GACCTTCAAAACTACTCTGT+GGG 0.522931 6.2:+53136327 MS.gene86868:CDS
TGTTGGTATTGTTGTGGCTG+GGG 0.523881 6.2:+53142426 MS.gene86868:CDS
AGCATGGTCCCACATGTACA+AGG 0.525900 6.2:+53132465 MS.gene86868:CDS
GCTACCCTTGTCTGTACACG+TGG 0.527539 6.2:-53132546 None:intergenic
GTTTGGGAGGTGGAATTGTA+CGG 0.529132 6.2:-53142372 None:intergenic
GCTTGAAGAATCCTTGAAGC+AGG 0.530577 6.2:+53131690 MS.gene86868:CDS
TCAATCGATGATAAATGGAT+AGG 0.531348 6.2:-53131301 None:intergenic
CATTCGGCTTCATGGATGCA+AGG 0.531966 6.2:+53131563 MS.gene86868:CDS
TGTCAAAGGTCTGACGGAGC+AGG 0.532802 6.2:+53132117 MS.gene86868:CDS
CCACATGTACAAGGGAAGGA+GGG 0.534382 6.2:+53132474 MS.gene86868:CDS
TGACAATCTTCCAACGGTAA+GGG 0.535333 6.2:+53140480 MS.gene86868:CDS
ACAATAATCTTCAGCAGACA+AGG 0.535930 6.2:+53132592 MS.gene86868:CDS
TAGCCAGCCGAATTTGTCTC+AGG 0.536300 6.2:+53131231 MS.gene86868:CDS
CAGAGCCAAGCCAGTCAGGA+AGG 0.538115 6.2:+53132887 MS.gene86868:CDS
GCCATTGTTGTTTGAGCACG+AGG 0.538613 6.2:+53138421 MS.gene86868:CDS
AGCAGGAGGAGCTCAAAGAA+GGG 0.541661 6.2:+53133030 MS.gene86868:CDS
TTGGGATCCCTGGAGACGTA+TGG 0.543368 6.2:+53131342 MS.gene86868:CDS
AGGTGGTTGAGAATCGAGGC+AGG 0.544260 6.2:-53138476 None:intergenic
CAGACAAGGGTAGCAAATCA+AGG 0.547086 6.2:+53132555 MS.gene86868:CDS
GCAAGCAAATGGCTGGTTGG+TGG 0.547132 6.2:+53131919 MS.gene86868:CDS
TGGCTTCTTAACACATTCAT+CGG 0.547795 6.2:-53138402 None:intergenic
TCCGGCAGCAGACGAGAGGA+GGG 0.547816 6.2:+53131955 MS.gene86868:CDS
AATAAGCTGAGAAAGTGCTT+GGG 0.548791 6.2:-53140447 None:intergenic
TACGTACCTTATTGCCAGGA+AGG 0.548812 6.2:-53138644 None:intergenic
AGAACTGATATTGCTAAAGA+AGG 0.550390 6.2:+53133068 MS.gene86868:CDS
GAATTCTTGCAGGCAGTCCG+TGG 0.550451 6.2:+53131434 MS.gene86868:CDS
ACCCGCATGTATCTCCTCCA+TGG 0.551351 6.2:-53131652 None:intergenic
GTCTTGTGACTCTATGGCAA+AGG 0.554595 6.2:+53138590 MS.gene86868:CDS
TCTTGTGACTCTATGGCAAA+GGG 0.557620 6.2:+53138591 MS.gene86868:CDS
CCTCCTTCCCTTGTACATGT+GGG 0.558287 6.2:-53132473 None:intergenic
CACATGTACAAGGGAAGGAG+GGG 0.558796 6.2:+53132475 MS.gene86868:CDS
CAGCCTGAGACAAATTCGGC+TGG 0.560757 6.2:-53131234 None:intergenic
AACAGAACAAAGGCGTCGGT+TGG 0.562414 6.2:+53138530 MS.gene86868:CDS
CTTTCATGGCTAGTGCACGT+GGG 0.562727 6.2:+53132988 MS.gene86868:CDS
CTGGTCTCGGCAGAAGGCAT+CGG 0.563568 6.2:-53138499 None:intergenic
GAGGCTAATGCTTCCAGCAG+TGG 0.564032 6.2:+53131838 MS.gene86868:CDS
AGGAAAATAACTATGGTCCA+TGG 0.564345 6.2:+53131635 MS.gene86868:CDS
TGAAAGAACCAGGCTGCACA+GGG 0.566393 6.2:-53132971 None:intergenic
CCCACATGTACAAGGGAAGG+AGG 0.567746 6.2:+53132473 MS.gene86868:CDS
TTCCTCCAACACCTCGACTC+CGG 0.568357 6.2:-53134140 None:intergenic
ATGCCCTACCGTCATCATTG+AGG 0.568489 6.2:-53131204 None:intergenic
CTTGCATCCATGAAGCCGAA+TGG 0.570271 6.2:-53131562 None:intergenic
ATAAGTTCACAGAATGTCAA+AGG 0.572462 6.2:+53132103 MS.gene86868:CDS
GTCTCGGCAGAAGGCATCGG+AGG 0.572571 6.2:-53138496 None:intergenic
TTGATATTAGTTTGGAGACA+AGG 0.572633 6.2:+53132067 MS.gene86868:CDS
GTTGAGCCCACAAAGTTCAA+TGG 0.572824 6.2:+53132371 MS.gene86868:CDS
GCAGGGATAGCCGGTTTGGG+AGG 0.572989 6.2:-53142385 None:intergenic
AGATTACATTCGAGAGAACA+TGG 0.573453 6.2:+53140377 MS.gene86868:intron
GAGCAGGTTTAGTAAGGTGG+TGG 0.573972 6.2:+53132133 MS.gene86868:CDS
CATTAGTGTTAACTTCTGTA+GGG 0.573973 6.2:-53132167 None:intergenic
CGTGGGGCCAACAGAACTGG+TGG 0.574806 6.2:+53133005 MS.gene86868:CDS
AATATTGAAGAAAGTGATGG+TGG 0.575162 6.2:+53131477 MS.gene86868:CDS
TGATATTAGTTTGGAGACAA+GGG 0.575523 6.2:+53132068 MS.gene86868:CDS
TGCCAGGAAGGGTGCCAACA+AGG 0.577702 6.2:-53138632 None:intergenic
TATGGCGGCATAGTGGTACT+TGG 0.578206 6.2:+53139705 MS.gene86868:CDS
GGTATCAAGCAAACAAGTTG+AGG 0.578951 6.2:+53139099 MS.gene86868:intron
GGCTTCATGGATGCAAGGGT+CGG 0.579240 6.2:+53131568 MS.gene86868:CDS
ATTGCAAAGAGTCTCATCGC+AGG 0.579378 6.2:-53142403 None:intergenic
CAAACCGGCAAGCAAATGGC+TGG 0.579750 6.2:+53131912 MS.gene86868:CDS
TTGTCGCTGTTGTTGGAGAG+AGG 0.581813 6.2:+53142052 MS.gene86868:CDS
TGGAAACCTCCATGCTGTCA+TGG 0.582962 6.2:-53139276 None:intergenic
TCTGTGCTTAGTTGCTAAAG+TGG 0.585635 6.2:+53131267 MS.gene86868:CDS
ATTCTTGTTTGAACATGGCG+GGG 0.586014 6.2:+53132299 MS.gene86868:CDS
TGGTCCCACGTGTACAGACA+AGG 0.587218 6.2:+53132541 MS.gene86868:CDS
CCGGCAAGCAAATGGCTGGT+TGG 0.587307 6.2:+53131916 MS.gene86868:CDS
CCATGGTGCATGAAAGAGAT+CGG 0.588161 6.2:+53140506 MS.gene86868:CDS
CAGAACTGGTGGGCAGCAGG+AGG 0.588529 6.2:+53133016 MS.gene86868:CDS
GATAGATTCTTGTTTGAACA+TGG 0.588642 6.2:+53132294 MS.gene86868:CDS
TGAACCTCAATGATGACGGT+AGG 0.592809 6.2:+53131200 MS.gene86868:CDS
GCGCTGGTTGGGAGTCACAT+AGG 0.593164 6.2:+53131592 MS.gene86868:CDS
CCACGTGAGCAAAGAAATGG+AGG 0.596335 6.2:+53132510 MS.gene86868:CDS
TTCTTGTTTGAACATGGCGG+GGG 0.596453 6.2:+53132300 MS.gene86868:CDS
TTTCATGGCTAGTGCACGTG+GGG 0.599101 6.2:+53132989 MS.gene86868:CDS
GGATCAAATTAATCAAGCGT+TGG 0.604060 6.2:+53132251 MS.gene86868:CDS
ACGTCCATAAATGCTATGCA+TGG 0.605115 6.2:-53132037 None:intergenic
GAGCCCACAAAGTTCAATGG+TGG 0.605407 6.2:+53132374 MS.gene86868:CDS
GTGCGGATGATCTTTCGCAA+TGG 0.606859 6.2:+53134058 MS.gene86868:CDS
AGTCACGTGGAAAGAAGGAA+TGG 0.607031 6.2:+53132434 MS.gene86868:CDS
TTGCGAAAGATCATCCGCAC+AGG 0.607465 6.2:-53134055 None:intergenic
GAATTCCGGCAGCAGACGAG+AGG 0.607877 6.2:+53131951 MS.gene86868:CDS
ACGTGGAAAGAAGGAATGGA+AGG 0.608073 6.2:+53132438 MS.gene86868:CDS
ACGTACCTTATTGCCAGGAA+GGG 0.608544 6.2:-53138643 None:intergenic
AATCAAATATGGCGGCATAG+TGG 0.609379 6.2:+53139698 MS.gene86868:CDS
GGTCCCACATGTACAAGGGA+AGG 0.610405 6.2:+53132470 MS.gene86868:CDS
ACGGAGCAGGTTTAGTAAGG+TGG 0.610494 6.2:+53132130 MS.gene86868:CDS
AGCCCACAAAGTTCAATGGT+GGG 0.612458 6.2:+53132375 MS.gene86868:CDS
ACCATGGTCTCCCTTACCGT+TGG 0.613177 6.2:-53140490 None:intergenic
TGGCACCAGGGCTAACAAGA+GGG 0.613643 6.2:-53136302 None:intergenic
ACATGTACAAGGGAAGGAGG+GGG 0.613788 6.2:+53132476 MS.gene86868:CDS
GAACCTCAATGATGACGGTA+GGG 0.614952 6.2:+53131201 MS.gene86868:CDS
CAACAATTAATGTTAATCAA+GGG 0.615420 6.2:+53131980 MS.gene86868:CDS
CGTCTCCAGGGATCCCAAAG+TGG 0.616010 6.2:-53131337 None:intergenic
CCGATCTCTTTCATGCACCA+TGG 0.616534 6.2:-53140506 None:intergenic
AGAAGGTGATCAAGGAAAGG+AGG 0.616742 6.2:+53133085 MS.gene86868:CDS
TTGCCACTGTTAATAATGTG+TGG 0.617420 6.2:-53142014 None:intergenic
TCCATGGAGGAGATACATGC+GGG 0.617705 6.2:+53131651 MS.gene86868:CDS
GAATTGGAAGCAACCTATCG+AGG 0.617927 6.2:+53142095 MS.gene86868:CDS
AGACATTGAAGTGTGTCGAA+AGG 0.620721 6.2:+53134110 MS.gene86868:CDS
TAATGAGTCAGAAAATGAGC+GGG 0.622524 6.2:+53132185 MS.gene86868:CDS
GAGATACATGCGGGTCAGAG+TGG 0.623389 6.2:+53131660 MS.gene86868:CDS
ATTCGGCTTCATGGATGCAA+GGG 0.626732 6.2:+53131564 MS.gene86868:CDS
CTTCTGCCGAGACCAGTCAC+CGG 0.626773 6.2:+53138506 MS.gene86868:CDS
AGATTCTTGTTTGAACATGG+CGG 0.627379 6.2:+53132297 MS.gene86868:CDS
AAGGAGTTGAACCGGAGTCG+AGG 0.627550 6.2:+53134129 MS.gene86868:CDS
CTTGTGATCGAAAGAGAAGG+TGG 0.629476 6.2:+53131715 MS.gene86868:CDS
GATAATATTGAAGAAAGTGA+TGG 0.629867 6.2:+53131474 MS.gene86868:CDS
CGTGGAAAGAAGGAATGGAA+GGG 0.629992 6.2:+53132439 MS.gene86868:CDS
ACCTCGTGCTCAAACAACAA+TGG 0.630611 6.2:-53138422 None:intergenic
ATGGCACCAGGGCTAACAAG+AGG 0.630670 6.2:-53136303 None:intergenic
TCATAAGTTGTCAGCCTGTG+CGG 0.631259 6.2:+53134041 MS.gene86868:CDS
CACGTGAGCAAAGAAATGGA+GGG 0.631318 6.2:+53132511 MS.gene86868:CDS
GTGGAAAGAAGGAATGGAAG+GGG 0.631395 6.2:+53132440 MS.gene86868:CDS
TTGCAAAGAGTCTCATCGCA+GGG 0.632149 6.2:-53142402 None:intergenic
ACTTCATTCTTGTCACCATG+TGG 0.634295 6.2:-53132414 None:intergenic
GCACTAGCCATGAAAGAACC+AGG 0.634631 6.2:-53132981 None:intergenic
GCGTCGGTTGGAGTTACCGA+AGG 0.636138 6.2:+53138542 MS.gene86868:CDS
ATTAATTCCATACGTCTCCA+GGG 0.636388 6.2:-53131349 None:intergenic
CATGCTGAAAATAAGGCCGT+CGG 0.639267 6.2:+53131883 MS.gene86868:CDS
AACTTGAACCTCAATGATGA+CGG 0.640725 6.2:+53131196 MS.gene86868:CDS
GGTGGTTGAGAATCGAGGCA+GGG 0.641183 6.2:-53138475 None:intergenic
GACCTTGTGATCGAAAGAGA+AGG 0.641759 6.2:+53131712 MS.gene86868:CDS
TTTGAAGGTCCATGGCACCA+GGG 0.643050 6.2:-53136314 None:intergenic
CTTCTTAACACATTCATCGG+TGG 0.643798 6.2:-53138399 None:intergenic
ATATTGAAGAAAGTGATGGT+GGG 0.644933 6.2:+53131478 MS.gene86868:CDS
TGTGAAATTTCTGAAACCAG+AGG 0.647784 6.2:+53139126 MS.gene86868:CDS
GTCCATGGAGGAGATACATG+CGG 0.648968 6.2:+53131650 MS.gene86868:CDS
CCGGCAGCAGACGAGAGGAG+GGG 0.650225 6.2:+53131956 MS.gene86868:CDS
GGACCTTCAAAACTACTCTG+TGG 0.653916 6.2:+53136326 MS.gene86868:CDS
CGTGAGCAAAGAAATGGAGG+GGG 0.654919 6.2:+53132513 MS.gene86868:CDS
CAATAATCTTCAGCAGACAA+GGG 0.655932 6.2:+53132593 MS.gene86868:CDS
ACACATTATTAACAGTGGCA+AGG 0.656178 6.2:+53142016 MS.gene86868:CDS
TCCAACGGTAAGGGAGACCA+TGG 0.656744 6.2:+53140489 MS.gene86868:CDS
TGGAAAGAAGGAATGGAAGG+GGG 0.658772 6.2:+53132441 MS.gene86868:CDS
TAAAGAAGGTGATCAAGGAA+AGG 0.659814 6.2:+53133082 MS.gene86868:CDS
CAAGCAGCGCAAGTTTACAC+TGG 0.660049 6.2:+53132941 MS.gene86868:CDS
GGTAGAAGGTATGACAGAGA+GGG 0.660104 6.2:+53132272 MS.gene86868:CDS
AATTCTTGCAGGCAGTCCGT+GGG 0.661805 6.2:+53131435 MS.gene86868:CDS
ACAGAATGTCAAAGGTCTGA+CGG 0.663634 6.2:+53132111 MS.gene86868:CDS
AAAGAAGCAATCAAATATGG+CGG 0.664006 6.2:+53139690 MS.gene86868:CDS
TCGGAGGTGGTTGAGAATCG+AGG 0.664452 6.2:-53138480 None:intergenic
TGCCACTGTTAATAATGTGT+GGG 0.664757 6.2:-53142013 None:intergenic
TGCAATAAAGGCTGACCGTG+TGG 0.665831 6.2:-53132618 None:intergenic
TCCAGCAGTGGAAAGAAATG+GGG 0.666616 6.2:+53131850 MS.gene86868:CDS
ACGTGAGCAAAGAAATGGAG+GGG 0.670280 6.2:+53132512 MS.gene86868:CDS
CGGAAACAGAACAAAGGCGT+CGG 0.672227 6.2:+53138526 MS.gene86868:CDS
TGGTAGAAGGTATGACAGAG+AGG 0.673514 6.2:+53132271 MS.gene86868:CDS
ATATTATTATCCAGTACCCA+CGG 0.674092 6.2:-53131451 None:intergenic
GCATGGTCCCACATGTACAA+GGG 0.675731 6.2:+53132466 MS.gene86868:CDS
CTACCCTTGTCTGTACACGT+GGG 0.676542 6.2:-53132545 None:intergenic
GTGCTTAGTTGCTAAAGTGG+TGG 0.677219 6.2:+53131270 MS.gene86868:CDS
GTGGGGCCAACAGAACTGGT+GGG 0.678435 6.2:+53133006 MS.gene86868:CDS
CAGCAGACAAGGGCACCACA+CGG 0.679404 6.2:+53132603 MS.gene86868:CDS
CTAATGAGTCAGAAAATGAG+CGG 0.679555 6.2:+53132184 MS.gene86868:CDS
GTCCCACACATTATTAACAG+TGG 0.689036 6.2:+53142011 MS.gene86868:CDS
ACCTTCAAAACTACTCTGTG+GGG 0.692677 6.2:+53136328 MS.gene86868:CDS
GTGACAAGAATGAAGTCACG+TGG 0.693065 6.2:+53132421 MS.gene86868:CDS
GGTTGAGAATCGAGGCAGGG+TGG 0.702885 6.2:-53138472 None:intergenic
TGTGACTCTATGGCAAAGGG+AGG 0.706949 6.2:+53138594 MS.gene86868:CDS
AGTCACCGGAAACAGAACAA+AGG 0.708423 6.2:+53138520 MS.gene86868:CDS
GGTCCCACGTGTACAGACAA+GGG 0.709538 6.2:+53132542 MS.gene86868:CDS
AAAATAACTATGGTCCATGG+AGG 0.712688 6.2:+53131638 MS.gene86868:CDS
GAAAACAATAAAGTGCCACA+TGG 0.740832 6.2:+53132399 MS.gene86868:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.2 gene 53131190 53142477 53131190 ID=MS.gene86868
chr6.2 mRNA 53131190 53142477 53131190 ID=MS.gene86868.t1;Parent=MS.gene86868
chr6.2 exon 53131190 53133176 53131190 ID=MS.gene86868.t1.exon1;Parent=MS.gene86868.t1
chr6.2 CDS 53131190 53133176 53131190 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53133923 53134150 53133923 ID=MS.gene86868.t1.exon2;Parent=MS.gene86868.t1
chr6.2 CDS 53133923 53134150 53133923 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53136286 53136366 53136286 ID=MS.gene86868.t1.exon3;Parent=MS.gene86868.t1
chr6.2 CDS 53136286 53136366 53136286 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53138376 53138659 53138376 ID=MS.gene86868.t1.exon4;Parent=MS.gene86868.t1
chr6.2 CDS 53138376 53138659 53138376 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53139100 53139147 53139100 ID=MS.gene86868.t1.exon5;Parent=MS.gene86868.t1
chr6.2 CDS 53139100 53139147 53139100 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53139232 53139291 53139232 ID=MS.gene86868.t1.exon6;Parent=MS.gene86868.t1
chr6.2 CDS 53139232 53139291 53139232 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53139651 53139743 53139651 ID=MS.gene86868.t1.exon7;Parent=MS.gene86868.t1
chr6.2 CDS 53139651 53139743 53139651 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53140379 53140527 53140379 ID=MS.gene86868.t1.exon8;Parent=MS.gene86868.t1
chr6.2 CDS 53140379 53140527 53140379 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53142005 53142116 53142005 ID=MS.gene86868.t1.exon9;Parent=MS.gene86868.t1
chr6.2 CDS 53142005 53142116 53142005 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
chr6.2 exon 53142352 53142477 53142352 ID=MS.gene86868.t1.exon10;Parent=MS.gene86868.t1
chr6.2 CDS 53142352 53142477 53142352 ID=cds.MS.gene86868.t1;Parent=MS.gene86868.t1
Gene Sequence

>MS.gene86868

ATGGAGAACTTGAACCTCAATGATGACGGTAGGGCATCCAATAGCCAGCCGAATTTGTCTCAGGCTGAGCTGTTGGATCTGTGCTTAGTTGCTAAAGTGGTGGTAAACAAACCTATCCATTTATCATCGATTGAATCAAGATTAGGTCCACTTTGGGATCCCTGGAGACGTATGGAATTAATTCCAATTCCAACAGAGAATAACAAATTTTTGGTCCAACTTTTCCATAAAGGTGATCTGGACAGAATTCTTGCAGGCAGTCCGTGGGTACTGGATAATAATATGATAATATTGAAGAAAGTGATGGTGGGAGAAGATCCTATTGCCGTTCCAATGAACTCAACAGAAATTTGGGTGCAAGTTCATCAGCTCCCATTCGGCTTCATGGATGCAAGGGTCGGAGCGCTGGTTGGGAGTCACATAGGAAAAATGGTCAGATATGATGAGGAAAATAACTATGGTCCATGGAGGAGATACATGCGGGTCAGAGTGGAGATTGGGCTTGAAGAATCCTTGAAGCAGGACCTTGTGATCGAAAGAGAAGGTGGTGAAGATATAAAGCTTGTGTTCAAGTATGAACATCTTGGTAAGTTTTGTTTCATTTGTGGTTTAATTGGCCACACTGAAAATTTTTGCAATCACAAATACGAGGCTAATGCTTCCAGCAGTGGAAAGAAATGGGGTCCTTATTTGCATGCTGAAAATAAGGCCGTCGGCGATGACAAACCGGCAAGCAAATGGCTGGTTGGTGGTCGGGGAAAGAATTCCGGCAGCAGACGAGAGGAGGGGACAACAATTAATGTTAATCAAGGGAGTCATTCAAATTCAAATAATGGTTGTGGAAAGTCCATGCATAGCATTTATGGACGTGTTAAAGTTGATATTAGTTTGGAGACAAGGGCATTAACTTTCTATAAGTTCACAGAATGTCAAAGGTCTGACGGAGCAGGTTTAGTAAGGTGGTGGACTGAGATTGACCCTACAGAAGTTAACACTAATGAGTCAGAAAATGAGCGGGTCAAAAATAAGAACGATGTTTTTGCTCCAAACCTTACAAAGTCGGATCAAATTAATCAAGCGTTGGTAGAAGGTATGACAGAGAGGGATAGATTCTTGTTTGAACATGGCGGGGGTGCAGAAGCATATTGGGCATTGATTGAAAAAGAAGCACAAGAAGCTAGGTTGAGCCCACAAAGTTCAATGGTGGGAGAAAACAATAAAGTGCCACATGGTGACAAGAATGAAGTCACGTGGAAAGAAGGAATGGAAGGGGGCAGCATGGTCCCACATGTACAAGGGAAGGAGGGGGATAGACTGCTCCCACGTGAGCAAAGAAATGGAGGGGGCAACTTGGTCCCACGTGTACAGACAAGGGTAGCAAATCAAGGACAGCTTGTTTTACACAATAATCTTCAGCAGACAAGGGCACCACACGGTCAGCCTTTATTGCAGTCAGTATTGGTTAATGATAAAAAGAAAATGTCAGCACCTGATTTTCGTCAACTATTTGGCAATAAGTCAGCAGAGAATAATGTTGCTTTTAAGGTGAGTAAAGCTCTTTTGCAGGTGCAAACAGTGGAGAACAGTCAAAATGTAATTAATGTGCAATCAACAGTGATGTCCCCTGTTTTTAAGGCTGTGGATTATCGTGTTCTCTTTGACTCTACCACCAATAAAGACAAAAATGTGCAGCCAAGCAGAGCCAAGCCAGTCAGGAAGGATAAAGCACCAACTCACTTCCAACCAAACAACAAGCAGCGCAAGTTTACACTGGCACGCAACCCTGTGCAGCCTGGTTCTTTCATGGCTAGTGCACGTGGGGCCAACAGAACTGGTGGGCAGCAGGAGGAGCTCAAAGAAGGGCCTAAAAAGAGGCCCAGAACTGATATTGCTAAAGAAGGTGATCAAGGAAAGGAGGACATGCAAGTAGAAAGAGCTAGAAATAATAATGATGTAATTGTTCTTAGAAATAATCCTGTTTTTGAGGGTAATGATATATCGGCGGGTCCTGGTCACCAGGCCTGTCGGGAAAAAATAGTCTCGTCAGTTGGAACTGCCGGGGCCTGGGAAGCCCAAGTGTAGTTCCAAAAATCAAATTCCTTGTACGGTACTACAATCCCAATATCCTTTTTCTAAGTGAGACGTTGATCCACACTAATAAAATCGAGGAATTCCGTTATTTATTGGGTTTTGATTATTGTTTTGCTCCGGATAGGATAGGAAGAGGTGGAGGCATCGCTTTGTTTTGGCGAAAAACAATTAATTGCAATATTGTCAACTATTCGTCTAATCATATTAGTGCAAGAATTGAATAGGGTAACCATGGCCCTTGGATACTCACAGGGTTTTATGGATATCCGGAAAGTGGGAGAAGAAGGGAATCTTGGAATTTTATACGAAGTCTTGCCAGGAATATCAATCTCCCATGGTGCATTTTTGGTGATTTCAACGATATCTTGCATGCCCAAGAGAAAAAAGGGCGTGCGATTAGGCCAAATTGGCTTATTCGTGGTTTTAGACAAGCTATTCAGGAAGCTGGCCTCATTGACATTCATATGGAGGGTTACCCGTACACTTGGTTCAAAAGCTTAGGTACCCCTCGAGCCGTGGAGGAAACGTTAGATCGAGCATTAGCTAATGAAGCTTGGATGCAATTGTTTCCACATGTCAGATTAGAGAATCTTGCTGCCCCTTCTTCTGATCATTTTCCAATTTTGCTTGAAAGAACACCAGTGGTGCGCCCTACTCGAATAAAAAGAACTTTCAAGTTTGAGAATGATTGGAGAATAGAAGAAGGAGTGAATGAGGTTGTTAAAGATAGTTGGCAGAGTAATAGTGGTTCAGATGTCATTCATAAGTTGTCAGCCTGTGCGGATGATCTTTCGCAATGGAGTAAATCTCATTGCAACAAACTTCGTATAGACATTGAAGTGTGTCGAAAGGAGTTGAACCGGAGTCGAGGTGTTGGAGGAATTCAAGATGAACTTCATTTTGAGACCTTGAGGAAGAAACTTGATCATTTACTCATCCAAGATGATATGTATTGGAGGCAAAGGGCCAAAACGCATTGGTATCGAGATGGGGATTTGAATACAAAGTTTTTCACGCTGCAGCAACATCTAGGAAAAAAGTTAACAGGATTTTAGCTTTGGAAAATGATGATGGTGTGCGTATTTCTGACGAGGTGGGAATGCGCTCAATTGCAAAAAATTACTTTGAGGAATTATTTCAGGAAAAGGAGAGCTCTCGTAATCCGGACGTCAATTTGCTAAGTCAGGTCATCAGTATAGAGGATAACGAGCAACTAACAACTCCTTTTTGTATTAAAGAATTCAAAGAAGCGATGTTTTCTATGCACCCTGACAAATGTACGGGACCAGACGACTTTAATCCAGTTTTTTATCAGCATTTTTGGAACACGTGTAGTGATGATATCTTCCATGAATGTTGTCAATGGCTTAATGAAGGTCATTTTCCGCCGTCTCTGAATTCCACAAACATAGCCTTGATACCAAAAGGTTCAGAACAGAGATCTATGAAAGATTGGAGGTCCATAACACTTTGTAATGTGTTGTATAAGTTGTTGGCTAAAGTCTTAGCCAATCGTCTAAAATTGATTCTGCATAAGTGTATTGCAGATACTCAGTCTGCTTTTGTACCGGGTAGATCTATTTTGGACAATGCACTAGTGGCAATAGAAGTGGTGCATTATATGAATACTAAACCACGAGGAAAGGACAGAAGTGTGGCACTAAAGCTGGACATAAGTAAAGCTTATGATAGGATAGATATAACACTTTGTAATGTGTTGTATAAGTTGTTGGCTAAAGTCTTAGCCAATCGTCTAAAATTGATTCTGCATAAGTGTATTGCAGATACTCAGTCTGCTTTTGTACCGGGTAGATCTATTTTGGAAAATGCACTAGTGGCAATAGAAGTGGTGCATTATATGAATACTAAACCACGAGGAAAGGACAGAAGTGTGGCACTAAAGCTGGACATAAGTAAAGCTTATGATAGGATAGATTGGTCCTACCTTAAAGATGTGATGCATAAGATGGGTTTCTCTGACAAGTGGATTAGTTGGATTATGATGTGTGTTGAGACAGTTGATTATTCATTTATTGTGAACAAAGACATGGTAGGCCCTATCATCCCAGGGAGAGGATTACGGCAGGGTGATCCTTTGTCGCCATATTTGTTTATTCTTTGTGCTGAAGGATTGTCAGCTTTAATCAGAAATGCTGAATCACGAGGAGAACTCCAAGGGATTCGAATTTGCAACGTTGCTCCCGTAGTCTCTCATTTACTCTTTGCCGATGACTGCTTTTTGTTCTTCAAAGCAGACGAGAACCAGGCAAATGTGATGAAGCAAATCTTATTGAATTACGAGGAAGCTTCAGGGCAAGCGATTAGTCTCCCCAAATCAGAAATATTTTATAGCAAGAATGTTGAGCAGCCACTCCAACAGGTCATCACAAATATTCTAGGGGTGCGTGCGGTTTTAGGCACTGGAAAGTATATTGGACTGCCATCTATGGTGGGGCGTAACAAAAAAGCAACGTTTAGTTTTATCAAGGATAGAGTGTGGCAAAGGATAAGCAGTTGGAGCAACAAGTGCTTATCCAAAGCAGGGAGGGAAGTCATGATCAAGTTTGTCCTTCAAGCTATTCCTTCATATGTTATGAGTATCTTCAGACTGCCAAATACATTATTGGACGAAATTGAAAAGATGATGAATGCATTCTGGTGGGGACATGGAGGTGCAAGCAACAAAGGTCTGCATTGGTTATCCTGGGAAAAGTTATCAGTGCACAAAAACCATGGAGGTATGGGCTTCAAAGATCTGGCAGCCTTTAATGTTGCAATGCTTGGAAAACAGGGTTGGAAGCTACAAACTGATTCAGATAGTTTAGTTTCACGACTTTTTAAATCTAAGTATTATCCGAACAGCAGTTACTTGAATTCGAAATTGGACCATAACCCAAGTTTTGTGTGGCGCAGTATCCATAGCGCGAAGGTGGTTGTCAGGCAAGGTGCTCGTTGGAAAATTGGGTCTGGATTTAATATTCCTATTATTGGTGAACCTTGGATAGAGACAGGATTGAGTATCCCTCTTGTTAGCCCTGGTGCCATGGACCTTCAAAACTACTCTGTGGGGCATTTGATTGATCAAAGTAATAAAGTCTGGAATGAACAGCTAATAAGGCAATTATTTTTAGCAGAAACAGCTCAAAGTATTTTGAATACTCCTTTATATTCACAAGTGCATACCGATGGTTTGCTCTGGAAGGCCGAGAAGAATGGCTGTTACTCGGTCAAAAGTGCCTACCGTATATGTATTGAAGATATTGCAAACAATGCTCATCTGCGGAAACCAGGATATTGGAGTGGTATATGGAGATTGAAAGTACCCCCAAGAGTGAAGAATTTAGTGTGGAGAGTGTGTAGAGAATGCTTCCCTACAAGGGTAAGGTTGATAAGTAGAGGAGTTAATTGTCCATCAGCTTGTGTTAAGTGTGATGATCCACATGAGGATAGCTATCACATATTTTTTCATTGCACGACTGCCATAGATGCTTGGAATGCTGCAAATGTCTGGCATTTGATTGCACCATCCCTGAATCAGTTTGATAATGCACCAGACATTATATTTAATCTATTGCAAAAGTTGTCGGCAAGTCAAATGGAGAGTATTGTTACAATAATGTGGAGTATCTGGAAGTCCCGTAATTTAAAGCTGTGGCAACAGGTGTCAGAGTCGACAGTTACAATTCTGGAAAGAGCTAAGCATCTCCTAGAAGGATGGAGGAAGGCGAATCGAAAGCAGGGTCTCTTAGGTCAACCTCACTCACCTACAAGCATAGGTCCTCAATCTCAGGGTGGTCAGAATACGGATAACAGGCAAGGTAGTACCCGATGGAGGAAACCAAGGAGTGGTAGACTCAAATGCAATGTCGATGCCTCTTTTTCTACATCCAGTAATAAAGTAGGCATAGGCATGTGTATTCGCAATTCAGAAGGAAACCACGTTCGTTCCAAGACCATGTGGTTTTCACCACTATGTCCAGTTGACATTGGAGAAGCTTTGGGACTTTATCATGCAATTCGGCGGATTAATGAACTTCAGCTCACAAATGTTGATTTTGAGGTAGATTCGAAAACAGTAGCTGATTATTTTAACAAAGGCAGAGGAGATAGCACCGAATTTGGATCCATTATGGATAAAAGTATCCAGATTTGTAATACCTTTTTAACAAACTCTCATGTTGAGTTTATTAGGAGGCAAGCAAATGAGGTTGCTCATGAGTTAGCTAAGGCAACCACATTATTACCTAGCTTCCACATCTTTGATGAAATTCCAACATGTATTACTGATTTAATTTCTAATGAAATGATATAAGTTATTTTCCCTCAAAAAAAAAAAAAAAAGCCTACATTTTCTATCTCCACGTCACTCCTACGTACTTCTCTTCTATTTCTCTCTTCACTAACCCTCTCTTCTTTGAACTCTTTCAAACCAAACAGAGTGTTAATGACTAGAATTTAATGCATAGAAATGGGCTATATAAACACACATTTTAATGATGGTTTAGGAGGGAAAAACTTTGCTTGATCAGTATTTAGCAATAAGCAAACTTTTCCCCCAAAATCAACCGTACACAAAGATTATTCATATATATATAAACCCAAATCAGAACGTGTGGCTCCACTCCAGCTCTGTCTCCTACCGCCGTAACTCACCTCCGTCAATCCACCAAAACCCTAATCACTCCACCTTCTTCTGCAAATCGCAACCATTTTCTGACTCCACCAGAAACCCAAATCGTGAAACCTGTTCCATTTTTGTGTTCTAGTTCGTACCTGTTCTTCATCCATTTTCTGGTACACTTTTCATTCTTATTCTTATTTTCTTCTTTTTTTTTTTTTATTATTGCTTCATCATTTTAAAACTGTTGTGTTTATTTTGCATCTGTTTCGGTTATGTGTGTTAATGTGTTTTGGTTACTAGATTATGGGTTTTTTTTTCTTCCAAAATTAGAAGGGTTTTTTTGTTGTTGTTAAGAATCTGTATTGTATTGTACATTGTTGTTTCGATTGAGCTTTTTATATATTGTTGTTTTCAATTTCGTTGTGTGCGGTTGAATTGCGCTTGACTTGTGTGTGTTTTTGTGTGAGATTTCAGCTTTGATTAAGCATCTTCAGGCGCCACCGATGAATGTGTTAAGAAGCCATTGTTGTTTGAGCACGAGGTTGAGAAAGGTTATCCAAACAACATTCACCACCCTGCCTCGATTCTCAACCACCTCCGATGCCTTCTGCCGAGACCAGTCACCGGAAACAGAACAAAGGCGTCGGTTGGAGTTACCGAAGGAGCTTACGAAAAATGTCATCCAACTGTCTTGTGACTCTATGGCAAAGGGAGGTGTTTGCGATGTCTACCTTGTTGGCACCCTTCCTGGCAATAAGGTACGTAAGAAATTCGTCTCAAAACTTTAAAAAGGGTTTATGTTTATGGACAAATTTTATCTTTCAAAGAGTGATTCTAATTAGAGACGGATTTAAAAGACCGATACTCCTGTGTATCTAAATACTATACTCATCGAGTTAATCATTGAGATTAAGAAAGTTGGTTATGATGTTAAATTTGTTGACTATTGATTTTGCTTTTACAAATTTATCTTCTATATGAGTAAATATAATGATTAATGTGGTTGAAAATTCAAAGATAATCTTATAAAATGGGATAAAGATAAAACAAATCAGGTCTTATATTTAGGTTTGTTAACACCATATGGAATGTATTTCATTCTTTTATCATACGAGTAGTTGTATTTATGAGACATTCAATTTTTTTTATCATCATCATCATCATCATCGGAAGTCCCTTGTTTTGAACTATATTGCAGGTATCAAGCAAACAAGTTGAGGAGATTGTGAAATTTCTGAAACCAGAGGTGGTTTTGTCCTTTTGATTTCAAGCTTTCAGTTCCCTTATTATCTTTGTACATTTTTTAATGCTTGGCTTTTGTCTACTCCAGATTGTTTTCTTGCAATTGTGCTCAGATAGTCAGGAAGTGCTTTTCCATGACAGCATGGAGGTTTCCACTCTTTTAGTTCATATTTTAGATAATGCAATCATGACAATTAGGGGTAGCTTTTCTTGTATTGTATTGATAATCAACATTATCATAGTATAATTAATTTCTTTAATTTCTTACAGGCACTTACCATGGGAGGAATGAATGCAATGAGGAAGGAAGAATATAGCATCTCAAAAAATTATTAGCCGGTTGGATGCCAAGGCATGTTTCAATGTTTGACAATTATATTTATCATCCTATTACATTTGTCTACTATTAATCTTTCATTCTAACTTATTCCAGAATGATATTTGATTTTTGAAAAAAAAACCGTTGAAATTATCATGAACCATTTTTTTCCCTTTAAAATCTGACAGACAGAGGTCTATCCTTTTGCTGCGTTTCGTTCGGCATATAAAGAAGCAATCAAATATGGCGGCATAGTGGTACTTGGTGATCGCCGTTTAGAGGTTAGAATAATTTTTTATTTCATTTGTTTTTGAGGTCATGAATTTTTAGATCCATGAAAAACCACTGATAGTTCTTTAGAAAGTTTTCACTTGTTGATATTTTAATAAATCAATAATTATTAGGATAGTAAAATTTGTGACTTGAGAATGAATGCTATAGCCTATAAACCTGTGTTTTGTTAATGAGTTTCTATTTAAGACAAACCCATCATTAGAGTCTGAGTTTGATCTTTAGCTGAAACAATTTTTGGCCAAAATTTACATATCTCACGGCAAACTTTGGATTACTATAGTTCATTTCCCTTAGGCTTAGGAAACCAAGAAAAGAAGACAAATAACTCTATTTTTCTTGTCTTCTTTTGTAAGAATATCTCGTATCCCTTTTGCTGGCATAAAAGAGAAAAACAAAACTGAATGCTTTTCATAAAAGTTAAAAATGTAGCCTCTAAATCAAATGTTCTTAAAAATTAGCTATGTTTTTTTTGTCAAAAAAATCTTCAAATGCTCACTAATTATTTATTTCTATTATCAATGGTTAAAGTTTTACCAAATGTGCTTTTAGCTTGGTTTGCATGTGAGATTATGTTTTGCAACTTTGTTACTGTATTGATCGGTATTGATTTTGATTGAAACAGATTACATTCGAGAGAACATGGAGTAAGCTGTCACTTTGGCATATCACAAAATTATTGATTGCTAATTTCCCAAGCACTTTCTCAGCTTATTTATCCCTTTTTGACAATCTTCCAACGGTAAGGGAGACCATGGTGCATGAAAGAGATCGGTAAGTTCTAATATTGGAGTCTAGATAGATCCTCTCTATTTTGTACTTTCTCCATTTCTATCATTACCAAAGTTTTGAAAATATAAATGTGTTGTAAATTCCGCCTTCATTTTGGACCGCCCCTAGTCGCCTAAGCCGGTTTTGTAATGATGAGTTTGACCCCAAATTTCAACATGGTATCAGAGCCTATCCTAGATCCATCGTTGGTCTTCCCGCATCGTCAACGCTTCAGGCCCATTGGGCCGGGCTGAAGCATGAGGGGGTGCGTTGGAAATTCCGCCTTAATTGCGGTCTGCCCCTAGTTGCCTAAATTGCGACCTTTGGTTAGCCTTCATTGCATCCTGCAACACCTTCATTGTGTTGGCTTTGAGGGGACGGATTTAGTCCCACATGGTATAGATAGGACTCTTGAGAAGAGTTTATAAAGAGAAGGCACTCCTCACCTTACAAGCCGGTTCTGTGAGGATGAGTTAGGCTCCAAATTCCAACAAAATGCAATAATGTGACATTTTGTGAATACAATTGTTTAATATACACAACAAAACGTTTCAAACTATAGTTATGGAGACGAAAAAAGGAGAGAAAATTGAGAGGATCCTATCTCACTCCTAATATTGCACTTAATTAAATTTAAAGTTTGATTTATTGTGATCGTCACCAGCTGTAATTTTTGTGGCTGTTTATTTTTTCCCATAACAAGTTGAAAAATTCTCCTTTCCTCTCTTGTACTTTTTCCTTCCTTCGCACAAAGTTTTTCCCTCCGAAGTCTGAAACACAGCCTAAAGAATACATACGTTTAACTGGAGGGGCTCTTGGATAGCATCTTGAATAAGGTTATTGTTTTGGCATTTTCTGCTTATCGTAGGCAACCTGAAGGTCCAAATTCAAATACCTTGGGTGCCTATTTTGTTTCATAGCACATGATATGAAAATACTGTAGAATAGACCCTACTGCAGTGATTTTATAGAGGTTGATATCAGTTAAACGTTTTTATCTCACTCTCAAAAGTTAGTTCCATGAATGAGTGTGATGGCACTCTGAAGTTTAGACAAGGCAATTTCTTTATGTATGCAATTAACATTAAACCGTAGTCCCTCGATGATGATGAGAAATTAACAGTTTGAAACACCCTACTATGTACAATCTGAAGAATAGAGATATTGGTAAGGGTCATGAATGCTAAAGCTACTTTAAAAACCATGTTTGGGTGGCCTTTCTTTTGATATATATGCCTATGGTGTTCTTGTAACTTACAAAATCTAAGGAAAGCTTATGCAAATACGCTAAAAACAAAATACATATCATACATTGTTTTTATAAGCTCTCTCAAATAGTCTCACAAGTGCTTATATAACTAGATAAGAGCAAATAGATTAATCCAAACAAAGTCTGTGTTTGGTATTTGTTTACTATAAATTATATAGTTATGATGCATCTCATATTATGCATGACTAAATTTAGACTGCGTCTATCTAGGTACATGTCCCACACATTATTAACAGTGGCAAGGAAGAGCAGAACAGTTGTCGCTGTTGTTGGAGAGAGGCATCTAGAAGGAATGAAGAAGAATTGGAAGCAACCTATCGAGGTTGGTGAAAATTCTGTTATTTATGTTATTTAATCATAATAAATGTTAGAATGTACCGTTTTTACACTTCAAAAGTATTTAGCACATGAAGAATTACTCAGCTACTCCTACTTGACTTGTGAATGAAAAAAACTGCTGATAAGTATTTTTGTCTCTTCTTTTTCAAAATGGTATTGTTATTCTTTTACTGGTCATTGAAAAATTTACTGACCCCAATTTTCCTTACTATTATCAGATCCAGGAGCTTCAGGAGCTCCGTACAATTCCACCTCCCAAACCGGCTATCCCTGCGATGAGACTCTTTGCAATTGTTGGTATTGTTGTGGCTGGGGTGACCATAATATCAACCATCTATCATTAA
Protein sequence

>MS.gene86868.t1

MENLNLNDDGRASNSQPNLSQAELLDLCLVAKVVVNKPIHLSSIESRLGPLWDPWRRMELIPIPTENNKFLVQLFHKGDLDRILAGSPWVLDNNMIILKKVMVGEDPIAVPMNSTEIWVQVHQLPFGFMDARVGALVGSHIGKMVRYDEENNYGPWRRYMRVRVEIGLEESLKQDLVIEREGGEDIKLVFKYEHLGKFCFICGLIGHTENFCNHKYEANASSSGKKWGPYLHAENKAVGDDKPASKWLVGGRGKNSGSRREEGTTINVNQGSHSNSNNGCGKSMHSIYGRVKVDISLETRALTFYKFTECQRSDGAGLVRWWTEIDPTEVNTNESENERVKNKNDVFAPNLTKSDQINQALVEGMTERDRFLFEHGGGAEAYWALIEKEAQEARLSPQSSMVGENNKVPHGDKNEVTWKEGMEGGSMVPHVQGKEGDRLLPREQRNGGGNLVPRVQTRVANQGQLVLHNNLQQTRAPHGQPLLQSVLVNDKKKMSAPDFRQLFGNKSAENNVAFKVSKALLQVQTVENSQNVINVQSTVMSPVFKAVDYRVLFDSTTNKDKNVQPSRAKPVRKDKAPTHFQPNNKQRKFTLARNPVQPGSFMASARGANRTGGQQEELKEGPKKRPRTDIAKEGDQGKEDMQVERARNNNDVIVLRNNPVFEVVRPTRIKRTFKFENDWRIEEGVNEVVKDSWQSNSGSDVIHKLSACADDLSQWSKSHCNKLRIDIEVCRKELNRSRETGLSIPLVSPGAMDLQNYSVGHLIDQTLIKHLQAPPMNVLRSHCCLSTRLRKVIQTTFTTLPRFSTTSDAFCRDQSPETEQRRRLELPKELTKNVIQLSCDSMAKGGVCDVYLVGTLPGNKVSSKQVEEIVKFLKPEIVFLQLCSDSQEVLFHDSMETEVYPFAAFRSAYKEAIKYGGIVVLGDRRLEITFERTWSKLSLWHITKLLIANFPSTFSAYLSLFDNLPTVRETMVHERDRYMSHTLLTVARKSRTVVAVVGERHLEGMKKNWKQPIEIQELQELRTIPPPKPAIPAMRLFAIVGIVVAGVTIISTIYH