AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95960.t1 MTR_1g036720 64.486 107 38 0 1 107 1 107 1.25e-46 153
MS.gene95960.t1 MTR_0010s0370 63.248 117 34 3 1 112 1 113 3.30e-45 152
MS.gene95960.t1 MTR_0010s0350 63.248 117 34 3 1 112 1 113 3.30e-45 152
MS.gene95960.t1 MTR_1g051330 86.047 43 6 0 259 301 326 368 5.97e-17 81.6
MS.gene95960.t1 MTR_2g061500 36.449 107 65 2 7 112 11 115 3.02e-14 68.9
MS.gene95960.t1 MTR_3g053920 35.514 107 66 2 4 109 8 112 1.42e-12 67.0
MS.gene95960.t1 MTR_6g046587 94.444 36 2 0 259 294 221 256 1.66e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 89 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTCGCTTAACGATAAT+TGG 0.220109 6.2:+76772248 None:intergenic
GGAGTTGATATTCAAGAAAT+AGG 0.223046 6.2:-76772079 MS.gene95960:CDS
ACTTTAGCACACAGTAAATT+AGG 0.239158 6.2:+76771425 None:intergenic
AACAGATTTGCCAGCGTTGC+TGG 0.250348 6.2:+76771455 None:intergenic
CACATGCTCTTGATTGGTCT+TGG 0.284616 6.2:+76771280 None:intergenic
CGCTCATCTGGTGGCGGTAT+TGG 0.289604 6.2:-76771548 MS.gene95960:CDS
CTACTGAAGTGTTGTTGTTT+TGG 0.309096 6.2:+76771498 None:intergenic
ACCTGTTTGGGATCGCTTGC+GGG 0.309802 6.2:+76771950 None:intergenic
TGGAGAAGATGGAAACCTTC+TGG 0.337321 6.2:-76772114 MS.gene95960:CDS
GTGGTTCCATTAGTAGCTTT+TGG 0.338638 6.2:+76771209 None:intergenic
TTCTCCTAATGATGCAGCTT+CGG 0.346673 6.2:-76771402 MS.gene95960:CDS
GCTGGCTTAGGAGTGGTTAT+AGG 0.358933 6.2:+76771473 None:intergenic
CATCTTCTCCATCATCATAT+AGG 0.372338 6.2:+76772126 None:intergenic
TCTCCTAATGATGCAGCTTC+GGG 0.375119 6.2:-76771401 MS.gene95960:CDS
TTAGTTAAGAACCGACCAAC+AGG 0.382430 6.2:+76772163 None:intergenic
ATGACAACGACACTAGACTA+TGG 0.387682 6.2:-76771598 MS.gene95960:CDS
TTTGCCAGCGTTGCTGGCTT+AGG 0.389905 6.2:+76771461 None:intergenic
GAACTCTTGTTACTTCATCT+TGG 0.396519 6.2:-76770522 MS.gene95960:CDS
CTACCCGAAGCTGCATCATT+AGG 0.400621 6.2:+76771398 None:intergenic
ATGTTGGAGGGATACCTGTT+TGG 0.402637 6.2:+76771937 None:intergenic
TGTTGGAGGGATACCTGTTT+GGG 0.413390 6.2:+76771938 None:intergenic
AACCCTTGTGGCCCCGATCT+CGG 0.429162 6.2:-76770470 MS.gene95960:CDS
TGTAAATCAGCGGTGACATC+AGG 0.432984 6.2:+76772193 None:intergenic
TGTTTATCCACTCGCAGCTC+TGG 0.437082 6.2:+76771572 None:intergenic
GTGGATAAACAACGCTCATC+TGG 0.437618 6.2:-76771560 MS.gene95960:CDS
GCGGAGTATGATCCGAGATC+GGG 0.440635 6.2:+76770458 None:intergenic
GGGCAGCACATGCTCTTGAT+TGG 0.446869 6.2:+76771274 None:intergenic
GCAGCTTCGGGTAGTCATGA+TGG 0.455134 6.2:-76771389 MS.gene95960:CDS
GCCCGCAAGCGATCCCAAAC+AGG 0.458730 6.2:-76771951 MS.gene95960:intron
TCTTAACTAATCGCCCCGTC+AGG 0.462221 6.2:-76772150 MS.gene95960:CDS
GAGTTGATATTCAAGAAATA+GGG 0.467932 6.2:-76772078 MS.gene95960:CDS
CAGCTTCGGGTAGTCATGAT+GGG 0.472742 6.2:-76771388 MS.gene95960:CDS
AGGGTTTGAAGAGTTGCATT+CGG 0.477846 6.2:+76770487 None:intergenic
TACCTGTTTGGGATCGCTTG+CGG 0.478904 6.2:+76771949 None:intergenic
TGAATATCAACTCCCTTCAC+AGG 0.484987 6.2:+76772088 None:intergenic
CCCATACCAAAAGCTACTAA+TGG 0.485330 6.2:-76771215 MS.gene95960:CDS
TCAAGATTCGACTCATATCA+AGG 0.489016 6.2:+76772023 None:intergenic
TACATCTCCGCCCTGTTGGT+CGG 0.500145 6.2:-76772174 MS.gene95960:CDS
CAAGAGCATGTGCTGCCCAC+TGG 0.500165 6.2:-76771269 MS.gene95960:CDS
CACTCCTAAGCCAGCAACGC+TGG 0.501277 6.2:-76771465 MS.gene95960:CDS
GAAGAGTTGCATTCGGATAG+AGG 0.501465 6.2:+76770494 None:intergenic
TGAGACATCCATTGGAGTTG+TGG 0.502736 6.2:+76771190 None:intergenic
TGGGTCGTGAGCTAGGGAAC+AGG 0.506064 6.2:+76770418 None:intergenic
CTATATGATGATGGAGAAGA+TGG 0.509585 6.2:-76772125 MS.gene95960:CDS
TAATAACTACGAAGTCGAAA+CGG 0.514307 6.2:-76771337 MS.gene95960:CDS
GATTTACATCTCCGCCCTGT+TGG 0.515271 6.2:-76772178 MS.gene95960:CDS
GTGCTTTGTGAGACATCCAT+TGG 0.524590 6.2:+76771182 None:intergenic
AGCGGAGTATGATCCGAGAT+CGG 0.527319 6.2:+76770457 None:intergenic
ATGAGTCGAATCTTGAAGAA+AGG 0.527340 6.2:-76772016 MS.gene95960:CDS
CTACGAAGTCGAAACGGTCA+AGG 0.534000 6.2:-76771331 MS.gene95960:CDS
TGACAACGACACTAGACTAT+GGG 0.538783 6.2:-76771597 MS.gene95960:CDS
GTATTTCGACAACCATCTCT+TGG 0.539847 6.2:-76771987 MS.gene95960:CDS
TAATGGAACCACAACTCCAA+TGG 0.541760 6.2:-76771198 MS.gene95960:CDS
GATATTCAAGAAATAGGGCA+AGG 0.541809 6.2:-76772073 MS.gene95960:CDS
CGTCAGGTCCTATATGATGA+TGG 0.543457 6.2:-76772134 MS.gene95960:CDS
AACCTTCTGGAATCCTGTGA+AGG 0.546249 6.2:-76772101 MS.gene95960:CDS
GATCCGAGATCGGGGCCACA+AGG 0.548771 6.2:+76770467 None:intergenic
TACGAAGTCGAAACGGTCAA+GGG 0.552334 6.2:-76771330 MS.gene95960:CDS
GCTTAACGATAATTGGTGAA+TGG 0.553412 6.2:+76772255 None:intergenic
GTTACAAGAAGAAGATAATG+AGG 0.560706 6.2:-76771363 MS.gene95960:CDS
CAGCGTTGCTGGCTTAGGAG+TGG 0.564531 6.2:+76771466 None:intergenic
CATCTGGTGGCGGTATTGGA+CGG 0.564815 6.2:-76771544 MS.gene95960:CDS
ATCATCATATAGGACCTGAC+GGG 0.578944 6.2:+76772136 None:intergenic
ACCTTCTGGAATCCTGTGAA+GGG 0.585979 6.2:-76772100 MS.gene95960:CDS
CATCATCATATAGGACCTGA+CGG 0.595841 6.2:+76772135 None:intergenic
TCCCTTCACAGGATTCCAGA+AGG 0.603468 6.2:+76772099 None:intergenic
CAACGCTGGCAAATCTGTTG+AGG 0.603496 6.2:-76771451 MS.gene95960:CDS
TAATTGGTGAATGGAAGCCA+TGG 0.607189 6.2:+76772264 None:intergenic
AAACAACGCTCATCTGGTGG+CGG 0.616538 6.2:-76771554 MS.gene95960:CDS
AAATCAGCGGTGACATCAGG+TGG 0.622978 6.2:+76772196 None:intergenic
GGATCAAGTTGAAGCAAGAG+CGG 0.625486 6.2:+76770439 None:intergenic
TGATGTCAAGTACCAAGAGA+TGG 0.626519 6.2:+76771975 None:intergenic
ATCCGAGATCGGGGCCACAA+GGG 0.635429 6.2:+76770468 None:intergenic
GATAAACAACGCTCATCTGG+TGG 0.636074 6.2:-76771557 MS.gene95960:CDS
TGCAACTCTTCAAACCCTTG+TGG 0.641968 6.2:-76770482 MS.gene95960:CDS
ATATTCAAGAAATAGGGCAA+GGG 0.643201 6.2:-76772072 MS.gene95960:CDS
ATGGGGACCAGAGCTGCGAG+TGG 0.648897 6.2:-76771579 MS.gene95960:CDS
TAGTTAAGAACCGACCAACA+GGG 0.649526 6.2:+76772164 None:intergenic
TTGAAATCAGAATTTCCAGT+GGG 0.652448 6.2:+76771254 None:intergenic
CGGAGTATGATCCGAGATCG+GGG 0.663149 6.2:+76770459 None:intergenic
AGCTTCGGGTAGTCATGATG+GGG 0.678825 6.2:-76771387 MS.gene95960:CDS
TTTGAAATCAGAATTTCCAG+TGG 0.684673 6.2:+76771253 None:intergenic
GACAACGACACTAGACTATG+GGG 0.703649 6.2:-76771596 MS.gene95960:CDS
AGATGGTTGTCGAAATACCA+CGG 0.714644 6.2:+76771992 None:intergenic
TCTAACTCTAACAATCAGCA+CGG 0.715794 6.2:-76771305 MS.gene95960:CDS
TCATCATATAGGACCTGACG+GGG 0.720009 6.2:+76772137 None:intergenic
AGGGCGGAGATGTAAATCAG+CGG 0.727163 6.2:+76772183 None:intergenic
GAATCTTGAAGAAAGGACCG+TGG 0.751100 6.2:-76772009 MS.gene95960:CDS
TTAAGAACCGACCAACAGGG+CGG 0.789695 6.2:+76772167 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCAAAATGAATATTATATCT+TGG - chr6.2:76771572-76771591 MS.gene95960:CDS 20.0%
! GATGATTTATGTTACACTAT+TGG - chr6.2:76771709-76771728 MS.gene95960:intron 25.0%
! TAATATCCAGTTTATAGACT+CGG + chr6.2:76771651-76771670 None:intergenic 25.0%
!! AAAGACATAGCTAACTTTTT+GGG - chr6.2:76770837-76770856 MS.gene95960:intron 25.0%
!! GATATAATATTCATTTTGCC+CGG + chr6.2:76771571-76771590 None:intergenic 25.0%
!!! AATGTCATTTTTCTGTTGAT+AGG - chr6.2:76771737-76771756 MS.gene95960:intron 25.0%
!!! GAGTTGATATTCAAGAAATA+GGG - chr6.2:76770613-76770632 MS.gene95960:intron 25.0%
AAAAAGATTAAGAAACCAGG+TGG - chr6.2:76770960-76770979 MS.gene95960:intron 30.0%
AAATTCAAGTATGAACGCTT+AGG - chr6.2:76770999-76771018 MS.gene95960:intron 30.0%
ACTTTAGCACACAGTAAATT+AGG + chr6.2:76771269-76771288 None:intergenic 30.0%
AGTTCCTTCAATTAAAGACT+TGG - chr6.2:76771624-76771643 MS.gene95960:intron 30.0%
ATATTCAAGAAATAGGGCAA+GGG - chr6.2:76770619-76770638 MS.gene95960:intron 30.0%
CAGAAAAAGATTAAGAAACC+AGG - chr6.2:76770957-76770976 MS.gene95960:intron 30.0%
GTTACAAGAAGAAGATAATG+AGG - chr6.2:76771328-76771347 MS.gene95960:CDS 30.0%
TAATAACTACGAAGTCGAAA+CGG - chr6.2:76771354-76771373 MS.gene95960:CDS 30.0%
TTGAAATCAGAATTTCCAGT+GGG + chr6.2:76771440-76771459 None:intergenic 30.0%
TTGTATGATTTACCTCTTCT+TGG - chr6.2:76772084-76772103 MS.gene95960:CDS 30.0%
! CTGGTTTCTTAATCTTTTTC+TGG + chr6.2:76770959-76770978 None:intergenic 30.0%
! GAAAGACATAGCTAACTTTT+TGG - chr6.2:76770836-76770855 MS.gene95960:intron 30.0%
! TACTTTTGAACCATGTGAAT+TGG + chr6.2:76772113-76772132 None:intergenic 30.0%
! TGTTGTTTAGATTTCTCAAG+AGG + chr6.2:76771936-76771955 None:intergenic 30.0%
! TTCACATGGTTCAAAAGTAT+CGG - chr6.2:76772114-76772133 MS.gene95960:CDS 30.0%
! TTTGAAATCAGAATTTCCAG+TGG + chr6.2:76771441-76771460 None:intergenic 30.0%
!! CATTTTTCTGTTGATAGGAT+TGG - chr6.2:76771742-76771761 MS.gene95960:intron 30.0%
!! GGAGTTGATATTCAAGAAAT+AGG - chr6.2:76770612-76770631 MS.gene95960:intron 30.0%
!!! ACAAAGCACTCCTTTTTTAT+CGG - chr6.2:76771523-76771542 MS.gene95960:CDS 30.0%
ATGAGTCGAATCTTGAAGAA+AGG - chr6.2:76770675-76770694 MS.gene95960:intron 35.0%
CATATATGAACGGAGGAAAT+TGG + chr6.2:76770895-76770914 None:intergenic 35.0%
CATCTTCTCCATCATCATAT+AGG + chr6.2:76770568-76770587 None:intergenic 35.0%
CTCTAATGCGCATATATGAA+CGG + chr6.2:76770905-76770924 None:intergenic 35.0%
GATATTCAAGAAATAGGGCA+AGG - chr6.2:76770618-76770637 MS.gene95960:intron 35.0%
GCTTAACGATAATTGGTGAA+TGG + chr6.2:76770439-76770458 None:intergenic 35.0%
TCAAGATTCGACTCATATCA+AGG + chr6.2:76770671-76770690 None:intergenic 35.0%
TCTAACTCTAACAATCAGCA+CGG - chr6.2:76771386-76771405 MS.gene95960:CDS 35.0%
! CATTAGTAGCTTTTGGTATG+GGG + chr6.2:76771478-76771497 None:intergenic 35.0%
! CCATTAGTAGCTTTTGGTAT+GGG + chr6.2:76771479-76771498 None:intergenic 35.0%
! GAACTCTTGTTACTTCATCT+TGG - chr6.2:76772169-76772188 MS.gene95960:CDS 35.0%
! TCCATTAGTAGCTTTTGGTA+TGG + chr6.2:76771480-76771499 None:intergenic 35.0%
!! CTATATGATGATGGAGAAGA+TGG - chr6.2:76770566-76770585 MS.gene95960:intron 35.0%
!!! CTACTGAAGTGTTGTTGTTT+TGG + chr6.2:76771196-76771215 None:intergenic 35.0%
!!! GGAAACTACTGTTACTTTTG+TGG - chr6.2:76771020-76771039 MS.gene95960:intron 35.0%
!!! TGTTACTTTTGTGGCATGTT+GGG - chr6.2:76771029-76771048 MS.gene95960:intron 35.0%
AATTTCCAACAAGAGAGTCC+TGG + chr6.2:76771855-76771874 None:intergenic 40.0%
ACAACATGTCCAATGAACCT+TGG - chr6.2:76771950-76771969 MS.gene95960:intron 40.0%
AGATGGTTGTCGAAATACCA+CGG + chr6.2:76770702-76770721 None:intergenic 40.0%
AGGACTCTCTTGTTGGAAAT+TGG - chr6.2:76771854-76771873 MS.gene95960:intron 40.0%
AGGGTTTGAAGAGTTGCATT+CGG + chr6.2:76772207-76772226 None:intergenic 40.0%
ATCATCATATAGGACCTGAC+GGG + chr6.2:76770558-76770577 None:intergenic 40.0%
ATGACAACGACACTAGACTA+TGG - chr6.2:76771093-76771112 MS.gene95960:intron 40.0%
ATTATATCTTGGAACTGCCG+AGG - chr6.2:76771583-76771602 MS.gene95960:CDS 40.0%
CAGGTTCATGACATTCCTAT+CGG - chr6.2:76770792-76770811 MS.gene95960:intron 40.0%
CATCATCATATAGGACCTGA+CGG + chr6.2:76770559-76770578 None:intergenic 40.0%
CATGCACTAGAGTTTCAATG+AGG + chr6.2:76771678-76771697 None:intergenic 40.0%
CATTAGAGGATTCCAACATG+CGG - chr6.2:76772047-76772066 MS.gene95960:CDS 40.0%
CCCATACCAAAAGCTACTAA+TGG - chr6.2:76771476-76771495 MS.gene95960:CDS 40.0%
CGGACCAAGTCTTTAATTGA+AGG + chr6.2:76771631-76771650 None:intergenic 40.0%
CTTCATCAATCTTTCGATCC+AGG - chr6.2:76771834-76771853 MS.gene95960:intron 40.0%
CTTCGTCGCTTAACGATAAT+TGG + chr6.2:76770446-76770465 None:intergenic 40.0%
GGTCTGATGTCGGAAAAAAT+CGG - chr6.2:76770813-76770832 MS.gene95960:intron 40.0%
GTATTTCGACAACCATCTCT+TGG - chr6.2:76770704-76770723 MS.gene95960:intron 40.0%
GTCTGATGTCGGAAAAAATC+GGG - chr6.2:76770814-76770833 MS.gene95960:intron 40.0%
TAATGCGCATATATGAACGG+AGG + chr6.2:76770902-76770921 None:intergenic 40.0%
TAATGGAACCACAACTCCAA+TGG - chr6.2:76771493-76771512 MS.gene95960:CDS 40.0%
TAGTTAAGAACCGACCAACA+GGG + chr6.2:76770530-76770549 None:intergenic 40.0%
TCATGAACCTGAATCCAGAA+GGG + chr6.2:76770783-76770802 None:intergenic 40.0%
TGAATATCAACTCCCTTCAC+AGG + chr6.2:76770606-76770625 None:intergenic 40.0%
TGACAACGACACTAGACTAT+GGG - chr6.2:76771094-76771113 MS.gene95960:intron 40.0%
TGATGTCAAGTACCAAGAGA+TGG + chr6.2:76770719-76770738 None:intergenic 40.0%
TGGTAGAAGGAGAGAATCTT+GGG - chr6.2:76771909-76771928 MS.gene95960:intron 40.0%
TTAGTTAAGAACCGACCAAC+AGG + chr6.2:76770531-76770550 None:intergenic 40.0%
TTCTCCTAATGATGCAGCTT+CGG - chr6.2:76771289-76771308 MS.gene95960:CDS 40.0%
TTCTTGGCTACCAATTCACA+TGG - chr6.2:76772100-76772119 MS.gene95960:CDS 40.0%
! AATGAACCTTGGTGCATCAT+CGG - chr6.2:76771961-76771980 MS.gene95960:CDS 40.0%
! GTGGTTCCATTAGTAGCTTT+TGG + chr6.2:76771485-76771504 None:intergenic 40.0%
! GTTGATAGGATTGGTAGAAG+TGG - chr6.2:76771751-76771770 MS.gene95960:intron 40.0%
! TAATATTCATTTTGCCCGGC+AGG + chr6.2:76771567-76771586 None:intergenic 40.0%
!! ATCTTTTTCTGGCGTTTGAG+AGG + chr6.2:76770948-76770967 None:intergenic 40.0%
!!! CTGTTACTTTTGTGGCATGT+TGG - chr6.2:76771028-76771047 MS.gene95960:intron 40.0%
AAATCTCCGATGATGCACCA+AGG + chr6.2:76771970-76771989 None:intergenic 45.0%
ACATGTTCCCTTCTGGATTC+AGG - chr6.2:76770773-76770792 MS.gene95960:intron 45.0%
ATGTAGTGTGAATCTCCACC+TGG + chr6.2:76770978-76770997 None:intergenic 45.0%
ATGTTGGAGGGATACCTGTT+TGG + chr6.2:76770757-76770776 None:intergenic 45.0%
CATTCCTATCGGTCTGATGT+CGG - chr6.2:76770803-76770822 MS.gene95960:intron 45.0%
CGTCAGGTCCTATATGATGA+TGG - chr6.2:76770557-76770576 MS.gene95960:intron 45.0%
CTCTCACCTGATGATAAACG+TGG - chr6.2:76772000-76772019 MS.gene95960:CDS 45.0%
CTGGTAGAAGGAGAGAATCT+TGG - chr6.2:76771908-76771927 MS.gene95960:intron 45.0%
CTTGGTCCGAGTCTATAAAC+TGG - chr6.2:76771642-76771661 MS.gene95960:intron 45.0%
GAAGAGTTGCATTCGGATAG+AGG + chr6.2:76772200-76772219 None:intergenic 45.0%
GAATCCAGAAGGGAACATGT+TGG + chr6.2:76770773-76770792 None:intergenic 45.0%
GAATCTTGAAGAAAGGACCG+TGG - chr6.2:76770682-76770701 MS.gene95960:intron 45.0%
GACAACGACACTAGACTATG+GGG - chr6.2:76771095-76771114 MS.gene95960:intron 45.0%
GATAAACAACGCTCATCTGG+TGG - chr6.2:76771134-76771153 MS.gene95960:intron 45.0%
GATGATGCACCAAGGTTCAT+TGG + chr6.2:76771962-76771981 None:intergenic 45.0%
GTCATGAACCTGAATCCAGA+AGG + chr6.2:76770784-76770803 None:intergenic 45.0%
GTGCTTTGTGAGACATCCAT+TGG + chr6.2:76771512-76771531 None:intergenic 45.0%
GTGGATAAACAACGCTCATC+TGG - chr6.2:76771131-76771150 MS.gene95960:intron 45.0%
GTTGGAAATTGGAGACTCAC+AGG - chr6.2:76771865-76771884 MS.gene95960:intron 45.0%
TACAATCAGTGACCGCATGT+TGG + chr6.2:76772062-76772081 None:intergenic 45.0%
TACGAAGTCGAAACGGTCAA+GGG - chr6.2:76771361-76771380 MS.gene95960:CDS 45.0%
TCACCTGATGATAAACGTGG+TGG - chr6.2:76772003-76772022 MS.gene95960:CDS 45.0%
TCATCATATAGGACCTGACG+GGG + chr6.2:76770557-76770576 None:intergenic 45.0%
TCTCCTAATGATGCAGCTTC+GGG - chr6.2:76771290-76771309 MS.gene95960:CDS 45.0%
TGAGACATCCATTGGAGTTG+TGG + chr6.2:76771504-76771523 None:intergenic 45.0%
TGATGATAAACGTGGTGGTC+CGG - chr6.2:76772008-76772027 MS.gene95960:CDS 45.0%
TGCAACTCTTCAAACCCTTG+TGG - chr6.2:76772209-76772228 MS.gene95960:CDS 45.0%
TGGAGAAGATGGAAACCTTC+TGG - chr6.2:76770577-76770596 MS.gene95960:intron 45.0%
TGGAGACTCACAGGTTTCTA+CGG - chr6.2:76771874-76771893 MS.gene95960:intron 45.0%
TGGCATGTTGGGACATATAG+AGG - chr6.2:76771040-76771059 MS.gene95960:intron 45.0%
TGTAAATCAGCGGTGACATC+AGG + chr6.2:76770501-76770520 None:intergenic 45.0%
TGTTGGAGGGATACCTGTTT+GGG + chr6.2:76770756-76770775 None:intergenic 45.0%
TTCTCTCCTTCTACCAGAGT+CGG + chr6.2:76771905-76771924 None:intergenic 45.0%
TTGGAATCCTCTAATGAGCC+AGG + chr6.2:76772043-76772062 None:intergenic 45.0%
! GATAGGATTGGTAGAAGTGG+TGG - chr6.2:76771754-76771773 MS.gene95960:intron 45.0%
! GTGAATTGGTAGCCAAGAAG+AGG + chr6.2:76772099-76772118 None:intergenic 45.0%
! TTTTCCGACATCAGACCGAT+AGG + chr6.2:76770810-76770829 None:intergenic 45.0%
!! AACCTTCTGGAATCCTGTGA+AGG - chr6.2:76770590-76770609 MS.gene95960:intron 45.0%
!! ACCTTCTGGAATCCTGTGAA+GGG - chr6.2:76770591-76770610 MS.gene95960:intron 45.0%
!! CACATGCTCTTGATTGGTCT+TGG + chr6.2:76771414-76771433 None:intergenic 45.0%
!! GGATCAAGTTGAAGCAAGAG+CGG + chr6.2:76772255-76772274 None:intergenic 45.0%
AAACAACGCTCATCTGGTGG+CGG - chr6.2:76771137-76771156 MS.gene95960:intron 50.0%
AAATCAGCGGTGACATCAGG+TGG + chr6.2:76770498-76770517 None:intergenic 50.0%
AACAGATTTGCCAGCGTTGC+TGG + chr6.2:76771239-76771258 None:intergenic 50.0%
AATCCTCTAATGAGCCAGGC+AGG + chr6.2:76772039-76772058 None:intergenic 50.0%
AGCGGAGTATGATCCGAGAT+CGG + chr6.2:76772237-76772256 None:intergenic 50.0%
AGCTTCGGGTAGTCATGATG+GGG - chr6.2:76771304-76771323 MS.gene95960:CDS 50.0%
AGGGCGGAGATGTAAATCAG+CGG + chr6.2:76770511-76770530 None:intergenic 50.0%
ATCCTCTAATGAGCCAGGCA+GGG + chr6.2:76772038-76772057 None:intergenic 50.0%
ATCTAGACGAGCTTCCTTGG+AGG + chr6.2:76772148-76772167 None:intergenic 50.0%
CAACGCTGGCAAATCTGTTG+AGG - chr6.2:76771240-76771259 MS.gene95960:CDS 50.0%
CAGCTTCGGGTAGTCATGAT+GGG - chr6.2:76771303-76771322 MS.gene95960:CDS 50.0%
CCAGAAGGGAACATGTTGGA+GGG + chr6.2:76770769-76770788 None:intergenic 50.0%
CCAGGCTCTGCCGATAAAAA+AGG + chr6.2:76771536-76771555 None:intergenic 50.0%
CCTTAACATTCCCCAAGCCT+CGG + chr6.2:76771603-76771622 None:intergenic 50.0%
CTACCCGAAGCTGCATCATT+AGG + chr6.2:76771296-76771315 None:intergenic 50.0%
CTACGAAGTCGAAACGGTCA+AGG - chr6.2:76771360-76771379 MS.gene95960:CDS 50.0%
GATACTCCGACTCTGGTAGA+AGG - chr6.2:76771896-76771915 MS.gene95960:intron 50.0%
GATTTACATCTCCGCCCTGT+TGG - chr6.2:76770513-76770532 MS.gene95960:CDS 50.0%
GGACCACCACGTTTATCATC+AGG + chr6.2:76772009-76772028 None:intergenic 50.0%
GTGGTGGCATAGCTGTACTT+TGG - chr6.2:76771770-76771789 MS.gene95960:intron 50.0%
TCCAGAAGGGAACATGTTGG+AGG + chr6.2:76770770-76770789 None:intergenic 50.0%
TCCCTTCACAGGATTCCAGA+AGG + chr6.2:76770595-76770614 None:intergenic 50.0%
TCGATCCAGGACTCTCTTGT+TGG - chr6.2:76771847-76771866 MS.gene95960:intron 50.0%
TCGATCTAGACGAGCTTCCT+TGG + chr6.2:76772151-76772170 None:intergenic 50.0%
TCTTAACTAATCGCCCCGTC+AGG - chr6.2:76770541-76770560 MS.gene95960:CDS 50.0%
TGTTTATCCACTCGCAGCTC+TGG + chr6.2:76771122-76771141 None:intergenic 50.0%
TTAAGAACCGACCAACAGGG+CGG + chr6.2:76770527-76770546 None:intergenic 50.0%
TTCTACGGATACTCCGACTC+TGG - chr6.2:76771889-76771908 MS.gene95960:intron 50.0%
! AGTAGCTTTTGGTATGGGGC+TGG + chr6.2:76771474-76771493 None:intergenic 50.0%
! GCTGGCTTAGGAGTGGTTAT+AGG + chr6.2:76771221-76771240 None:intergenic 50.0%
! TACCTGTTTGGGATCGCTTG+CGG + chr6.2:76770745-76770764 None:intergenic 50.0%
!!! CCTTTTTTATCGGCAGAGCC+TGG - chr6.2:76771533-76771552 MS.gene95960:CDS 50.0%
ACGAGCTTCCTTGGAGGAAG+AGG + chr6.2:76772142-76772161 None:intergenic 55.0%
ATCTTGGAACTGCCGAGGCT+TGG - chr6.2:76771588-76771607 MS.gene95960:CDS 55.0%
CCCTCCAACATGTTCCCTTC+TGG - chr6.2:76770766-76770785 MS.gene95960:intron 55.0%
CCGAGGCTTGGGGAATGTTA+AGG - chr6.2:76771600-76771619 MS.gene95960:CDS 55.0%
CGGAGTATGATCCGAGATCG+GGG + chr6.2:76772235-76772254 None:intergenic 55.0%
GCAGCTTCGGGTAGTCATGA+TGG - chr6.2:76771302-76771321 MS.gene95960:CDS 55.0%
GCGGAGTATGATCCGAGATC+GGG + chr6.2:76772236-76772255 None:intergenic 55.0%
GGGCAGCACATGCTCTTGAT+TGG + chr6.2:76771420-76771439 None:intergenic 55.0%
TACATCTCCGCCCTGTTGGT+CGG - chr6.2:76770517-76770536 MS.gene95960:CDS 55.0%
TATCGGCACCTCTTCCTCCA+AGG - chr6.2:76772131-76772150 MS.gene95960:CDS 55.0%
TCTTGGAACTGCCGAGGCTT+GGG - chr6.2:76771589-76771608 MS.gene95960:CDS 55.0%
! ACCTGTTTGGGATCGCTTGC+GGG + chr6.2:76770744-76770763 None:intergenic 55.0%
! AGCTTTTGGTATGGGGCTGG+TGG + chr6.2:76771471-76771490 None:intergenic 55.0%
!! CATCTGGTGGCGGTATTGGA+CGG - chr6.2:76771147-76771166 MS.gene95960:intron 55.0%
!! TTTGCCAGCGTTGCTGGCTT+AGG + chr6.2:76771233-76771252 None:intergenic 55.0%
AACCCTTGTGGCCCCGATCT+CGG - chr6.2:76772221-76772240 MS.gene95960:CDS 60.0%
ATCCGAGATCGGGGCCACAA+GGG + chr6.2:76772226-76772245 None:intergenic 60.0%
CACTCCTAAGCCAGCAACGC+TGG - chr6.2:76771226-76771245 MS.gene95960:CDS 60.0%
CTTGGAACTGCCGAGGCTTG+GGG - chr6.2:76771590-76771609 MS.gene95960:CDS 60.0%
! CAAGAGCATGTGCTGCCCAC+TGG - chr6.2:76771422-76771441 MS.gene95960:CDS 60.0%
! TCATTTTGCCCGGCAGGCCT+GGG + chr6.2:76771561-76771580 None:intergenic 60.0%
! TTCATTTTGCCCGGCAGGCC+TGG + chr6.2:76771562-76771581 None:intergenic 60.0%
!! CAGCGTTGCTGGCTTAGGAG+TGG + chr6.2:76771228-76771247 None:intergenic 60.0%
!! CGCTCATCTGGTGGCGGTAT+TGG - chr6.2:76771143-76771162 MS.gene95960:intron 60.0%
ATGGGGACCAGAGCTGCGAG+TGG - chr6.2:76771112-76771131 MS.gene95960:intron 65.0%
CGCCCTGCCTGGCTCATTAG+AGG - chr6.2:76772033-76772052 MS.gene95960:CDS 65.0%
GATCCGAGATCGGGGCCACA+AGG + chr6.2:76772227-76772246 None:intergenic 65.0%
GCCCGCAAGCGATCCCAAAC+AGG - chr6.2:76770740-76770759 MS.gene95960:intron 65.0%
ATCGGCAGAGCCTGGCTCCC+AGG - chr6.2:76771541-76771560 MS.gene95960:CDS 70.0%
! ATGAGCCAGGCAGGGCGCTC+CGG + chr6.2:76772030-76772049 None:intergenic 70.0%
AGCCTGGCTCCCAGGCCTGC+CGG - chr6.2:76771549-76771568 MS.gene95960:CDS 75.0%
! GCCTGGCTCCCAGGCCTGCC+GGG - chr6.2:76771550-76771569 MS.gene95960:CDS 80.0%
!! GTGGTCCGGAGCGCCCTGCC+TGG - chr6.2:76772022-76772041 MS.gene95960:CDS 80.0%
! GCCCGGCAGGCCTGGGAGCC+AGG + chr6.2:76771554-76771573 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 76770429 76772284 76770429 ID=MS.gene95960
chr6.2 mRNA 76770429 76772284 76770429 ID=MS.gene95960.t1;Parent=MS.gene95960
chr6.2 exon 76771952 76772284 76771952 ID=MS.gene95960.t1.exon1;Parent=MS.gene95960.t1
chr6.2 CDS 76771952 76772284 76771952 ID=cds.MS.gene95960.t1;Parent=MS.gene95960.t1
chr6.2 exon 76771196 76771636 76771196 ID=MS.gene95960.t1.exon2;Parent=MS.gene95960.t1
chr6.2 CDS 76771196 76771636 76771196 ID=cds.MS.gene95960.t1;Parent=MS.gene95960.t1
chr6.2 exon 76770429 76770560 76770429 ID=MS.gene95960.t1.exon3;Parent=MS.gene95960.t1
chr6.2 CDS 76770429 76770560 76770429 ID=cds.MS.gene95960.t1;Parent=MS.gene95960.t1
Gene Sequence

>MS.gene95960

ATGGCTTCCATTCACCAATTATCGTTAAGCGACGAAGATGACGAACTTGAATTTCAGTTTGATGAACCACCTGATGTCACCGCTGATTTACATCTCCGCCCTGTTGGTCGGTTCTTAACTAATCGCCCCGTCAGGTCCTATATGATGATGGAGAAGATGGAAACCTTCTGGAATCCTGTGAAGGGAGTTGATATTCAAGAAATAGGGCAAGGGTTGTACACGTTTCAGTTTCATCATCACCTTGATATGAGTCGAATCTTGAAGAAAGGACCGTGGTATTTCGACAACCATCTCTTGGTACTTGACATCATGCCCGCAAGCGATCCCAAACAGGTATCCCTCCAACATGTTCCCTTCTGGATTCAGGTTCATGACATTCCTATCGGTCTGATGTCGGAAAAAATCGGGAAAGACATAGCTAACTTTTTGGGTGAATTTTTAGAGTATGATGCGAAAAACAGTTCCAATTTCCTCCGTTCATATATGCGCATTAGAGTACTGCTTGACATTGCTAAACCTCTCAAACGCCAGAAAAAGATTAAGAAACCAGGTGGAGATTCACACTACATTAAATTCAAGTATGAACGCTTAGGAAACTACTGTTACTTTTGTGGCATGTTGGGACATATAGAGGATTATTGTGAAAAGTTATATGATACAACAGATGACAACGACACTAGACTATGGGGACCAGAGCTGCGAGTGGATAAACAACGCTCATCTGGTGGCGGTATTGGACGGTCAGTTACAACAGTTCAAGCATGCCAAAACAACAACACTTCAGTAGCGCCTATAACCACTCCTAAGCCAGCAACGCTGGCAAATCTGTTGAGGAATCCTAATTTACTGTGTGCTAAAGTTTCTCCTAATGATGCAGCTTCGGGTAGTCATGATGGGGTGTTACAAGAAGAAGATAATGAGGAATTAATAACTACGAAGTCGAAACGGTCAAGGGTCTCTAACTCTAACAATCAGCACGGAACCAAGACCAATCAAGAGCATGTGCTGCCCACTGGAAATTCTGATTTCAAAAATTCAACCACCAGCCCCATACCAAAAGCTACTAATGGAACCACAACTCCAATGGATGTCTCACAAAGCACTCCTTTTTTATCGGCAGAGCCTGGCTCCCAGGCCTGCCGGGCAAAATGAATATTATATCTTGGAACTGCCGAGGCTTGGGGAATGTTAAGGCAGTTCCTTCAATTAAAGACTTGGTCCGAGTCTATAAACTGGATATTATAATCCTCATTGAAACTCTAGTGCATGATAATAAAATTGATGATTTATGTTACACTATTGGCTTTGAATGTCATTTTTCTGTTGATAGGATTGGTAGAAGTGGTGGCATAGCTGTACTTTGGAGAAATTCTATTAAATGCTCTCTGTTAAATTACTCTCAGAACTTCATCAATCTTTCGATCCAGGACTCTCTTGTTGGAAATTGGAGACTCACAGGTTTCTACGGATACTCCGACTCTGGTAGAAGGAGAGAATCTTGGGACCTCTTGAGAAATCTAAACAACATGTCCAATGAACCTTGGTGCATCATCGGAGATTTTAATGATCATCTCTCACCTGATGATAAACGTGGTGGTCCGGAGCGCCCTGCCTGGCTCATTAGAGGATTCCAACATGCGGTCACTGATTGTAATTTGTATGATTTACCTCTTCTTGGCTACCAATTCACATGGTTCAAAAGTATCGGCACCTCTTCCTCCAAGGAAGCTCGTCTAGATCGAACTCTTGTTACTTCATCTTGGCAAGACCTCTATCCGAATGCAACTCTTCAAACCCTTGTGGCCCCGATCTCGGATCATACTCCGCTCTTGCTTCAACTTGATCCTGTTCCCTAG
Protein sequence

>MS.gene95960.t1

MASIHQLSLSDEDDELEFQFDEPPDVTADLHLRPVGRFLTNRPVRSYMMMEKMETFWNPVKGVDIQEIGQGLYTFQFHHHLDMSRILKKGPWYFDNHLLVLDIMPASDPKQLYDTTDDNDTRLWGPELRVDKQRSSGGGIGRSVTTVQACQNNNTSVAPITTPKPATLANLLRNPNLLCAKVSPNDAASGSHDGVLQEEDNEELITTKSKRSRVSNSNNQHGTKTNQEHVLPTGNSDFKNSTTSPIPKATNGTTTPMDEARLDRTLVTSSWQDLYPNATLQTLVAPISDHTPLLLQLDPVP