AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008658


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008658.t1 MTR_3g053920 37.748 151 91 1 2 152 5 152 6.04e-34 123
MS.gene008658.t1 MTR_2g061500 41.322 121 71 0 3 123 6 126 1.13e-31 113
MS.gene008658.t1 MTR_0010s0370 30.286 175 106 4 4 162 3 177 6.99e-22 89.4
MS.gene008658.t1 MTR_0010s0350 30.286 175 106 4 4 162 3 177 6.99e-22 89.4
MS.gene008658.t1 MTR_6g033215 25.568 176 117 2 22 183 118 293 5.96e-17 78.2
MS.gene008658.t1 MTR_1g036720 28.037 107 75 1 5 109 4 110 8.67e-13 63.2
MS.gene008658.t1 MTR_7g011710 40.278 72 43 0 52 123 1 72 1.66e-12 63.5
MS.gene008658.t1 MTR_4g103530 43.478 69 39 0 55 123 4 72 9.15e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGATTCTCCTTATCAAAT+AGG 0.270270 3.4:+60270331 None:intergenic
AAATTAAGATAAACATTCTT+AGG 0.288740 3.4:+60270643 None:intergenic
ACCACTTAAACAAGATTTGC+AGG 0.310094 3.4:-60270463 MS.gene008658:CDS
GATCCAAGATGGTCGAAGTT+TGG 0.363524 3.4:+60270899 None:intergenic
ACATTCTTAGGAACCATTCC+TGG 0.380386 3.4:+60270655 None:intergenic
ACCTGCAAATCTTGTTTAAG+TGG 0.390056 3.4:+60270462 None:intergenic
CACTTCTTGGAGGCTGGTGA+AGG 0.397566 3.4:-60270809 MS.gene008658:CDS
AGGAACGGCTGGGCACTTCT+TGG 0.405137 3.4:-60270822 MS.gene008658:CDS
GGCTTATCTCAAGGAACGGC+TGG 0.406425 3.4:-60270833 MS.gene008658:CDS
AAAATATCTTTCTCAAAGAC+TGG 0.412326 3.4:+60270312 None:intergenic
AACGGCGGGACCTGTACTTC+AGG 0.413843 3.4:-60270289 MS.gene008658:CDS
AGGTATATGTTTCCAGTTGT+AGG 0.418631 3.4:+60270772 None:intergenic
TGGTAAGTACCTTGGAGAAC+TGG 0.421913 3.4:-60270556 MS.gene008658:CDS
TCTTGTGAGTGAATTGAAAC+AGG 0.428370 3.4:+60270752 None:intergenic
GTCATTGAAATTATTGCTCC+AGG 0.433628 3.4:-60270673 MS.gene008658:CDS
TTGTGAAATCCTATTTGATA+AGG 0.433773 3.4:-60270340 MS.gene008658:CDS
TGTATGTGGGCCTGAAGTAC+AGG 0.438936 3.4:+60270279 None:intergenic
ATCACAGTTATACCTACAAC+TGG 0.439649 3.4:-60270784 MS.gene008658:CDS
TGTCCAAACTTCGACCATCT+TGG 0.441314 3.4:-60270902 MS.gene008658:CDS
GACAGATGCGTCAAGGTTCA+AGG 0.450026 3.4:+60270921 None:intergenic
TTTCACAAGTACGGCCAGTA+TGG 0.453288 3.4:+60270355 None:intergenic
ACCATGGATATCGATCTTCA+TGG 0.464241 3.4:-60270970 MS.gene008658:CDS
CGATCTTCATGGTTTGACCT+TGG 0.465702 3.4:-60270959 MS.gene008658:CDS
TCTTCATGGTTTGACCTTGG+AGG 0.471139 3.4:-60270956 MS.gene008658:CDS
AACGGCTGGGCACTTCTTGG+AGG 0.476553 3.4:-60270819 MS.gene008658:CDS
TTTGTTCTCGTGGTGGAGAG+TGG 0.494715 3.4:-60270440 MS.gene008658:CDS
CAAATAGGATTTCACAAGTA+CGG 0.501950 3.4:+60270346 None:intergenic
GCTGGGCACTTCTTGGAGGC+TGG 0.505559 3.4:-60270815 MS.gene008658:CDS
AGCCAATCGCACATCCTTGT+CGG 0.509959 3.4:+60270852 None:intergenic
TGTGGAGTTCTTGACCATAC+TGG 0.511742 3.4:-60270369 MS.gene008658:CDS
TTGTTCTCGTGGTGGAGAGT+GGG 0.512414 3.4:-60270439 MS.gene008658:CDS
GATTTGCAGGTTTGTTCTCG+TGG 0.513963 3.4:-60270450 MS.gene008658:CDS
ACTTCTTGGAGGCTGGTGAA+GGG 0.518226 3.4:-60270808 MS.gene008658:CDS
TGGTTTGACCTTGGAGGACG+AGG 0.523972 3.4:-60270950 MS.gene008658:CDS
TGAAATTATTGCTCCAGGAA+TGG 0.527151 3.4:-60270668 MS.gene008658:CDS
ATCCGACAAGGATGTGCGAT+TGG 0.543661 3.4:-60270854 MS.gene008658:CDS
ATCTTGGATCATTGTCTGAT+CGG 0.544005 3.4:-60270886 MS.gene008658:CDS
CAGGTTGTTCACGAAGCGAA+AGG 0.546922 3.4:-60270505 MS.gene008658:CDS
ACATGATGAAGGGTGGACCT+TGG 0.561440 3.4:-60270717 MS.gene008658:CDS
GAAGCCATTGGTAAGTACCT+TGG 0.564143 3.4:-60270564 MS.gene008658:CDS
TTCTCCAAGGTACTTACCAA+TGG 0.565889 3.4:+60270560 None:intergenic
TGACGCTGAAAACATGATGA+AGG 0.566730 3.4:-60270728 MS.gene008658:CDS
ACAGTAAGAACACTATTCAC+AGG 0.584216 3.4:-60270524 MS.gene008658:CDS
TTGCAGGTTTGTTCTCGTGG+TGG 0.586242 3.4:-60270447 MS.gene008658:CDS
GGTCATAACCTCGTCCTCCA+AGG 0.596650 3.4:+60270942 None:intergenic
TGTGCGATTGGCTTATCTCA+AGG 0.596876 3.4:-60270842 MS.gene008658:CDS
AAGTTTGGACAGATGCGTCA+AGG 0.608480 3.4:+60270914 None:intergenic
GCTTATCTCAAGGAACGGCT+GGG 0.616971 3.4:-60270832 MS.gene008658:CDS
CAAAAGGTAAGTGAAGCCAT+TGG 0.619195 3.4:-60270576 MS.gene008658:intron
GATCAGACAATGATCCAAGA+TGG 0.620594 3.4:+60270888 None:intergenic
CGGATGCATGCTATCCGACA+AGG 0.624370 3.4:-60270866 MS.gene008658:CDS
TTACAGCTGTCATACGTCCA+AGG 0.637466 3.4:+60270700 None:intergenic
CATACTCGACCAGTTCTCCA+AGG 0.638239 3.4:+60270547 None:intergenic
GATTGGCTTATCTCAAGGAA+CGG 0.659712 3.4:-60270837 MS.gene008658:CDS
GACGCTGAAAACATGATGAA+GGG 0.686342 3.4:-60270727 MS.gene008658:CDS
GCTGAAAACATGATGAAGGG+TGG 0.699446 3.4:-60270724 MS.gene008658:CDS
ACCATGAAGATCGATATCCA+TGG 0.704727 3.4:+60270969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAGATAAACATTCTT+AGG + chr3.4:60270599-60270618 None:intergenic 15.0%
!!! CTTTGAGAAAGATATTTTAA+CGG - chr3.4:60270932-60270951 MS.gene008658:CDS 20.0%
!!! TTTTGAGTTTATACAACAAA+AGG - chr3.4:60270647-60270666 MS.gene008658:CDS 20.0%
!!! TTTTGTTGTATAAACTCAAA+AGG + chr3.4:60270648-60270667 None:intergenic 20.0%
! AAAATATCTTTCTCAAAGAC+TGG + chr3.4:60270930-60270949 None:intergenic 25.0%
! TTGTGAAATCCTATTTGATA+AGG - chr3.4:60270899-60270918 MS.gene008658:CDS 25.0%
!! CGATTTTGTTCAAATACAAA+AGG - chr3.4:60270826-60270845 MS.gene008658:CDS 25.0%
CAAATAGGATTTCACAAGTA+CGG + chr3.4:60270896-60270915 None:intergenic 30.0%
GTTGTATAAACTCAAAAGGA+AGG + chr3.4:60270644-60270663 None:intergenic 30.0%
!!! TGAGAAAGATATTTTAACGG+CGG - chr3.4:60270935-60270954 MS.gene008658:CDS 30.0%
ACAGTAAGAACACTATTCAC+AGG - chr3.4:60270715-60270734 MS.gene008658:CDS 35.0%
ACCACTTAAACAAGATTTGC+AGG - chr3.4:60270776-60270795 MS.gene008658:CDS 35.0%
AGGTATATGTTTCCAGTTGT+AGG + chr3.4:60270470-60270489 None:intergenic 35.0%
ATCACAGTTATACCTACAAC+TGG - chr3.4:60270455-60270474 MS.gene008658:CDS 35.0%
ATCTTGGATCATTGTCTGAT+CGG - chr3.4:60270353-60270372 MS.gene008658:CDS 35.0%
CTGGATTCTCCTTATCAAAT+AGG + chr3.4:60270911-60270930 None:intergenic 35.0%
GTCATTGAAATTATTGCTCC+AGG - chr3.4:60270566-60270585 MS.gene008658:CDS 35.0%
TCTTGTGAGTGAATTGAAAC+AGG + chr3.4:60270490-60270509 None:intergenic 35.0%
TGAAATTATTGCTCCAGGAA+TGG - chr3.4:60270571-60270590 MS.gene008658:CDS 35.0%
! ACCTGCAAATCTTGTTTAAG+TGG + chr3.4:60270780-60270799 None:intergenic 35.0%
!! GAAACTTTTTGCTATGTGTG+TGG - chr3.4:60270852-60270871 MS.gene008658:CDS 35.0%
!!! GAGAAAGATATTTTAACGGC+GGG - chr3.4:60270936-60270955 MS.gene008658:CDS 35.0%
ACATTCTTAGGAACCATTCC+TGG + chr3.4:60270587-60270606 None:intergenic 40.0%
CAAAAGGTAAGTGAAGCCAT+TGG - chr3.4:60270663-60270682 MS.gene008658:CDS 40.0%
GACGCTGAAAACATGATGAA+GGG - chr3.4:60270512-60270531 MS.gene008658:CDS 40.0%
GATCAGACAATGATCCAAGA+TGG + chr3.4:60270354-60270373 None:intergenic 40.0%
TGACGCTGAAAACATGATGA+AGG - chr3.4:60270511-60270530 MS.gene008658:CDS 40.0%
! TTCTCCAAGGTACTTACCAA+TGG + chr3.4:60270682-60270701 None:intergenic 40.0%
!! GATTGGCTTATCTCAAGGAA+CGG - chr3.4:60270402-60270421 MS.gene008658:CDS 40.0%
AAGTTTGGACAGATGCGTCA+AGG + chr3.4:60270328-60270347 None:intergenic 45.0%
CGATCTTCATGGTTTGACCT+TGG - chr3.4:60270280-60270299 MS.gene008658:CDS 45.0%
GAAGCCATTGGTAAGTACCT+TGG - chr3.4:60270675-60270694 MS.gene008658:CDS 45.0%
GATCCAAGATGGTCGAAGTT+TGG + chr3.4:60270343-60270362 None:intergenic 45.0%
GCTGAAAACATGATGAAGGG+TGG - chr3.4:60270515-60270534 MS.gene008658:CDS 45.0%
TCTTCATGGTTTGACCTTGG+AGG - chr3.4:60270283-60270302 MS.gene008658:CDS 45.0%
TGGTAAGTACCTTGGAGAAC+TGG - chr3.4:60270683-60270702 MS.gene008658:CDS 45.0%
TGTCCAAACTTCGACCATCT+TGG - chr3.4:60270337-60270356 MS.gene008658:CDS 45.0%
TGTGGAGTTCTTGACCATAC+TGG - chr3.4:60270870-60270889 MS.gene008658:CDS 45.0%
TTACAGCTGTCATACGTCCA+AGG + chr3.4:60270542-60270561 None:intergenic 45.0%
TTTCACAAGTACGGCCAGTA+TGG + chr3.4:60270887-60270906 None:intergenic 45.0%
! GATTTGCAGGTTTGTTCTCG+TGG - chr3.4:60270789-60270808 MS.gene008658:CDS 45.0%
!! TGTGCGATTGGCTTATCTCA+AGG - chr3.4:60270397-60270416 MS.gene008658:CDS 45.0%
ACATGATGAAGGGTGGACCT+TGG - chr3.4:60270522-60270541 MS.gene008658:CDS 50.0%
ACTTCTTGGAGGCTGGTGAA+GGG - chr3.4:60270431-60270450 MS.gene008658:CDS 50.0%
AGCCAATCGCACATCCTTGT+CGG + chr3.4:60270390-60270409 None:intergenic 50.0%
ATCCGACAAGGATGTGCGAT+TGG - chr3.4:60270385-60270404 MS.gene008658:CDS 50.0%
CAGGTTGTTCACGAAGCGAA+AGG - chr3.4:60270734-60270753 MS.gene008658:CDS 50.0%
CATACTCGACCAGTTCTCCA+AGG + chr3.4:60270695-60270714 None:intergenic 50.0%
GACAGATGCGTCAAGGTTCA+AGG + chr3.4:60270321-60270340 None:intergenic 50.0%
GCTTATCTCAAGGAACGGCT+GGG - chr3.4:60270407-60270426 MS.gene008658:CDS 50.0%
TGTATGTGGGCCTGAAGTAC+AGG + chr3.4:60270963-60270982 None:intergenic 50.0%
! TTGCAGGTTTGTTCTCGTGG+TGG - chr3.4:60270792-60270811 MS.gene008658:CDS 50.0%
! TTGTTCTCGTGGTGGAGAGT+GGG - chr3.4:60270800-60270819 MS.gene008658:CDS 50.0%
! TTTGTTCTCGTGGTGGAGAG+TGG - chr3.4:60270799-60270818 MS.gene008658:CDS 50.0%
CACTTCTTGGAGGCTGGTGA+AGG - chr3.4:60270430-60270449 MS.gene008658:CDS 55.0%
CGGATGCATGCTATCCGACA+AGG - chr3.4:60270373-60270392 MS.gene008658:CDS 55.0%
GGCTTATCTCAAGGAACGGC+TGG - chr3.4:60270406-60270425 MS.gene008658:CDS 55.0%
GGTCATAACCTCGTCCTCCA+AGG + chr3.4:60270300-60270319 None:intergenic 55.0%
TGGTTTGACCTTGGAGGACG+AGG - chr3.4:60270289-60270308 MS.gene008658:CDS 55.0%
AACGGCGGGACCTGTACTTC+AGG - chr3.4:60270950-60270969 MS.gene008658:CDS 60.0%
AACGGCTGGGCACTTCTTGG+AGG - chr3.4:60270420-60270439 MS.gene008658:CDS 60.0%
AGGAACGGCTGGGCACTTCT+TGG - chr3.4:60270417-60270436 MS.gene008658:CDS 60.0%
GCTGGGCACTTCTTGGAGGC+TGG - chr3.4:60270424-60270443 MS.gene008658:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 60270272 60270989 60270272 ID=MS.gene008658
chr3.4 mRNA 60270272 60270989 60270272 ID=MS.gene008658.t1;Parent=MS.gene008658
chr3.4 exon 60270624 60270989 60270624 ID=MS.gene008658.t1.exon1;Parent=MS.gene008658.t1
chr3.4 CDS 60270624 60270989 60270624 ID=cds.MS.gene008658.t1;Parent=MS.gene008658.t1
chr3.4 exon 60270272 60270592 60270272 ID=MS.gene008658.t1.exon2;Parent=MS.gene008658.t1
chr3.4 CDS 60270272 60270592 60270272 ID=cds.MS.gene008658.t1;Parent=MS.gene008658.t1
Gene Sequence

>MS.gene008658

ATGGATATCGATCTTCATGGTTTGACCTTGGAGGACGAGGTTATGACCTTGAACCTTGACGCATCTGTCCAAACTTCGACCATCTTGGATCATTGTCTGATCGGATGCATGCTATCCGACAAGGATGTGCGATTGGCTTATCTCAAGGAACGGCTGGGCACTTCTTGGAGGCTGGTGAAGGGTATCACAGTTATACCTACAACTGGAAACATATACCTGTTTCAATTCACTCACAAGAATGACGCTGAAAACATGATGAAGGGTGGACCTTGGACGTATGACAGCTGTAATCTAGTCATTGAAATTATTGCTCCAGGAATGGTTCCTAAGAATGTTTATCTTAATTTTCTGAACATTTCTGTGCAAGTGCCTTCCTTTTGAGTTTATACAACAAAAGGTAAGTGAAGCCATTGGTAAGTACCTTGGAGAACTGGTCGAGTATGACAGTAAGAACACTATTCACAGGTTGTTCACGAAGCGAAAGGTTCGAATCAATGTAATACAACCACTTAAACAAGATTTGCAGGTTTGTTCTCGTGGTGGAGAGTGGGTTTCGATTTTGTTCAAATACAAAAGGCTTGAAACTTTTTGCTATGTGTGTGGAGTTCTTGACCATACTGGCCGTACTTGTGAAATCCTATTTGATAAGGAGAATCCAGTCTTTGAGAAAGATATTTTAACGGCGGGACCTGTACTTCAGGCCCACATACAAAAATGA

Protein sequence

>MS.gene008658.t1

MDIDLHGLTLEDEVMTLNLDASVQTSTILDHCLIGCMLSDKDVRLAYLKERLGTSWRLVKGITVIPTTGNIYLFQFTHKNDAENMMKGGPWTYDSCNLVIEIIAPGMVPKNVYLNFLNISVQVSEAIGKYLGELVEYDSKNTIHRLFTKRKVRINVIQPLKQDLQVCSRGGEWVSILFKYKRLETFCYVCGVLDHTGRTCEILFDKENPVFEKDILTAGPVLQAHIQK