AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61651

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61651.t1 MTR_3g053920 63.091 317 79 3 1 317 1 279 3.20e-134 384
MS.gene61651.t1 MTR_2g061500 82.171 129 23 0 1 129 1 129 1.19e-75 229
MS.gene61651.t1 MTR_4g103530 49.565 230 66 5 57 286 3 182 9.23e-65 203
MS.gene61651.t1 MTR_7g011710 68.235 85 27 0 55 139 1 85 1.88e-35 127
MS.gene61651.t1 MTR_0010s0370 39.665 179 94 6 8 177 4 177 4.84e-31 116
MS.gene61651.t1 MTR_0010s0350 39.665 179 94 6 8 177 4 177 4.84e-31 116
MS.gene61651.t1 MTR_6g033215 35.526 152 96 1 29 178 122 273 6.33e-26 105
MS.gene61651.t1 MTR_1g036720 33.010 103 69 0 11 113 9 111 2.18e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 86 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGCCAGCAGATGTATTT+TGG 0.145079 4.1:-46982418 None:intergenic
CCAAGTCATCCTTTGGGGCT+TGG 0.225720 4.1:+46982474 MS.gene61651:CDS
ATTCTCGAACACTGTCTTAT+TGG 0.250606 4.1:+46981553 MS.gene61651:CDS
GTTCCAAACCTTTGCGTAAC+TGG 0.267932 4.1:-46982159 None:intergenic
CCTATGCCTTGACCTACTTT+TGG 0.278124 4.1:-46981868 None:intergenic
CCTTCTTCCAAGTCATCCTT+TGG 0.285004 4.1:+46982467 MS.gene61651:CDS
TTCATTCCTGCACAGCGATT+TGG 0.311905 4.1:-46983000 None:intergenic
CATCGACTTTGTGGTGAAAC+TGG 0.326996 4.1:-46981693 None:intergenic
CTGATGATGGCGACCGCAAT+TGG 0.328177 4.1:+46982121 MS.gene61651:CDS
CTTCTTCCAAGTCATCCTTT+GGG 0.347936 4.1:+46982468 MS.gene61651:CDS
CAGGTTCGGTTTCAAGGTTA+AGG 0.359894 4.1:-46981519 None:intergenic
CATTATCAGGTTCGGTTTCA+AGG 0.368403 4.1:-46981525 None:intergenic
CGATACGATCCATGGCAATA+TGG 0.373064 4.1:-46981759 None:intergenic
CAAGGCATAGGAAGCTTCTT+AGG 0.376769 4.1:+46981880 MS.gene61651:CDS
CAACCAACGGTTTGTGGCAG+CGG 0.377733 4.1:-46982181 None:intergenic
TCGAGAATGGCAGCATTATC+AGG 0.387245 4.1:-46981538 None:intergenic
CAAGTTCACGGTCTGCCTTT+CGG 0.389727 4.1:+46981835 MS.gene61651:CDS
TTTAGCTAGCACCGCATTCT+GGG 0.402038 4.1:-46982370 None:intergenic
AAACCAGTTACGCAAAGGTT+TGG 0.405741 4.1:+46982156 MS.gene61651:CDS
AGCGACTCTGACACGCATAT+TGG 0.407533 4.1:-46981944 None:intergenic
TGTCACAGGTGTGGATTGAT+TGG 0.423731 4.1:+46982066 MS.gene61651:intron
TGCTTCCAAAATACATCTGC+TGG 0.424718 4.1:+46982413 MS.gene61651:CDS
GTGATCGTGACCTTCTTGCC+TGG 0.434097 4.1:-46981643 None:intergenic
AGGTTCGGTTTCAAGGTTAA+GGG 0.437088 4.1:-46981518 None:intergenic
TGGTTCAGTGGGACAAAGCT+AGG 0.443161 4.1:-46981796 None:intergenic
TGATGATGGCGACCGCAATT+GGG 0.445254 4.1:+46982122 MS.gene61651:CDS
ATCTTGCAACCAACGGTTTG+TGG 0.450416 4.1:-46982187 None:intergenic
GTAACGGTCAAACACTGATT+TGG 0.459048 4.1:-46981665 None:intergenic
CCAAGCCCCAAAGGATGACT+TGG 0.469128 4.1:-46982474 None:intergenic
GCCATGGATCGTATCGCTCC+TGG 0.471951 4.1:+46981766 MS.gene61651:CDS
AATGGCAGCATTATCAGGTT+CGG 0.471986 4.1:-46981533 None:intergenic
TATAGACCAAATCGCTGTGC+AGG 0.476677 4.1:+46982994 MS.gene61651:intron
CAAGCAGGTGCTGCTTCTGT+TGG 0.476934 4.1:+46982243 MS.gene61651:CDS
CGGTCTCACCTTGTCTGAAG+AGG 0.477786 4.1:+46981489 MS.gene61651:CDS
TGTGACATAGAAGCAGGAAT+AGG 0.487822 4.1:-46982210 None:intergenic
CAACAGAAGCAGCACCTGCT+TGG 0.492986 4.1:-46982242 None:intergenic
TCATTAGATTCACTTGAAAG+TGG 0.496760 4.1:-46982510 None:intergenic
CCTTCCAACATTAACCTTGA+CGG 0.499725 4.1:+46981469 MS.gene61651:CDS
CTGCTGGCTTCTTCTTCCAC+AGG 0.507471 4.1:+46982429 MS.gene61651:CDS
CACCTTCAGTAGCTGCAGTT+CGG 0.513939 4.1:-46982266 None:intergenic
TTCTTCCAAGTCATCCTTTG+GGG 0.514970 4.1:+46982469 MS.gene61651:CDS
TCACTTGAAAGTGGAGCAGC+AGG 0.515569 4.1:-46982501 None:intergenic
CCAAAGGATGACTTGGAAGA+AGG 0.518466 4.1:-46982467 None:intergenic
CCCGAGTTAGATTCTGATGA+TGG 0.525609 4.1:+46982108 MS.gene61651:CDS
ATATCTCGTGCATGGAAGCC+AGG 0.533414 4.1:+46981625 MS.gene61651:CDS
GGATTCATCCAACCAAAAGT+AGG 0.533518 4.1:+46981856 MS.gene61651:CDS
AATAAGACAGTGTTCGAGAA+TGG 0.533967 4.1:-46981551 None:intergenic
TACGTGCAGCATCGACTTTG+TGG 0.538561 4.1:-46981702 None:intergenic
AACTGCAGCTACTGAAGGTG+TGG 0.539423 4.1:+46982269 MS.gene61651:CDS
TGCTGCACGTATCCTTGATG+AGG 0.540746 4.1:+46981714 MS.gene61651:CDS
GCAGGTAAGCCAAGCCCCAA+AGG 0.549880 4.1:-46982483 None:intergenic
GTTTGATGTGACATAGAAGC+AGG 0.552176 4.1:-46982216 None:intergenic
TGATAATTTCCATATTGCCA+TGG 0.555425 4.1:+46981750 MS.gene61651:CDS
GGTCTCACCTTGTCTGAAGA+GGG 0.564346 4.1:+46981490 MS.gene61651:CDS
GAACCGCTGCCACAAACCGT+TGG 0.569652 4.1:+46982178 MS.gene61651:CDS
TCAAGTCAGCTCCCCAATTG+CGG 0.573419 4.1:-46982134 None:intergenic
GAGACCGTCAAGGTTAATGT+TGG 0.573663 4.1:-46981473 None:intergenic
CTTTGTGGTGAAACTGGAAG+AGG 0.574937 4.1:-46981687 None:intergenic
GATGATGGCGACCGCAATTG+GGG 0.575879 4.1:+46982123 MS.gene61651:CDS
CCGTCAAGGTTAATGTTGGA+AGG 0.583449 4.1:-46981469 None:intergenic
TGCATGGAAGCCAGGCAAGA+AGG 0.591899 4.1:+46981633 MS.gene61651:CDS
ACCAGGAGCGATACGATCCA+TGG 0.592943 4.1:-46981767 None:intergenic
GGTTCAGTGGGACAAAGCTA+GGG 0.592996 4.1:-46981795 None:intergenic
TCACATCAAACTCACCAAGC+AGG 0.593385 4.1:+46982228 MS.gene61651:CDS
GCCATCATCAGAATCTAACT+CGG 0.598191 4.1:-46982109 None:intergenic
ACAAAGCTAGGGACGACACC+AGG 0.602101 4.1:-46981784 None:intergenic
GTATCCTTGATGAGGGTCCA+CGG 0.605875 4.1:+46981722 MS.gene61651:CDS
GGCCGAACTGCAGCTACTGA+AGG 0.610869 4.1:+46982264 MS.gene61651:CDS
ACAACCGTGGACCCTCATCA+AGG 0.613072 4.1:-46981726 None:intergenic
ACTTGAAACCAGTTACGCAA+AGG 0.615229 4.1:+46982151 MS.gene61651:CDS
ATCCAACCAAAAGTAGGTCA+AGG 0.617189 4.1:+46981862 MS.gene61651:CDS
CCAAAAGTAGGTCAAGGCAT+AGG 0.620618 4.1:+46981868 MS.gene61651:CDS
GCTGCACGTATCCTTGATGA+GGG 0.620671 4.1:+46981715 MS.gene61651:CDS
GGTGAAACTGGAAGAGGTAA+CGG 0.623079 4.1:-46981681 None:intergenic
ATCTAATGATGACATACAAG+AGG 0.624350 4.1:+46982524 MS.gene61651:CDS
GCGAGCGTATATCTCGTGCA+TGG 0.627560 4.1:+46981617 MS.gene61651:CDS
GCTAGTAGCTGAAGTACCTG+TGG 0.637214 4.1:-46982445 None:intergenic
TGTGCTTGCTGCCCAGAATG+CGG 0.637409 4.1:+46982359 MS.gene61651:CDS
CCATCATCAGAATCTAACTC+GGG 0.645117 4.1:-46982108 None:intergenic
GAATAGGATCTTGCAACCAA+CGG 0.654985 4.1:-46982194 None:intergenic
ACTGCAGCTACTGAAGGTGT+GGG 0.661239 4.1:+46982270 MS.gene61651:CDS
GGTTAAACCCTCTTCAGACA+AGG 0.669126 4.1:-46981497 None:intergenic
ATTGAATCTCACGATCAACA+AGG 0.669685 4.1:-46981582 None:intergenic
CAGACAAGGTGAGACCGTCA+AGG 0.670109 4.1:-46981483 None:intergenic
TTTGGTTGGATGAATCCGAA+AGG 0.672828 4.1:-46981850 None:intergenic
TGGAAATTATCATACAACCG+TGG 0.719997 4.1:-46981739 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAAAAAATTACAG+GGG + chr4.1:46982821-46982840 MS.gene61651:intron 10.0%
!! AACTTTAAATATGAAAAATT+GGG + chr4.1:46982036-46982055 MS.gene61651:intron 10.0%
!! GTAAATAAAAAAAAAATTAC+AGG + chr4.1:46982819-46982838 MS.gene61651:intron 10.0%
!! TAACTTTAAATATGAAAAAT+TGG + chr4.1:46982035-46982054 MS.gene61651:intron 10.0%
!! AATAAAAAAAAAATTACAGG+GGG + chr4.1:46982822-46982841 MS.gene61651:intron 15.0%
!! AATCAAAAAAAAAAAATTGC+AGG - chr4.1:46982655-46982674 None:intergenic 15.0%
!! ATCAAAAAAAAAAAATTGCA+GGG - chr4.1:46982654-46982673 None:intergenic 15.0%
!! TTGACTTTAATAGATATTAT+GGG + chr4.1:46982967-46982986 MS.gene61651:intron 15.0%
!!! AAATAAAATTGGTTTTTGTT+TGG - chr4.1:46982724-46982743 None:intergenic 15.0%
!!! ATCAAAACATTTTTTTTTAC+AGG - chr4.1:46982684-46982703 None:intergenic 15.0%
!!! TCAAAACATTTTTTTTTACA+GGG - chr4.1:46982683-46982702 None:intergenic 15.0%
!!! TTGTTAAGTTATTCATTTTT+GGG - chr4.1:46982894-46982913 None:intergenic 15.0%
!!! TTTGACTTTAATAGATATTA+TGG + chr4.1:46982966-46982985 MS.gene61651:intron 15.0%
!!! TTTGTTAAGTTATTCATTTT+TGG - chr4.1:46982895-46982914 None:intergenic 15.0%
!! ATAAAAAAAAAATTACAGGG+GGG + chr4.1:46982823-46982842 MS.gene61651:intron 20.0%
!! TCAAAAAAAAAAAATTGCAG+GGG - chr4.1:46982653-46982672 None:intergenic 20.0%
!!! CAAAACCAATTTTTTTTACA+GGG + chr4.1:46982794-46982813 MS.gene61651:intron 20.0%
!!! TCAAAACCAATTTTTTTTAC+AGG + chr4.1:46982793-46982812 MS.gene61651:intron 20.0%
!!! TTATTCATTTTTGGGTTAAT+AGG - chr4.1:46982886-46982905 None:intergenic 20.0%
! AAAAATCAATATGGTTCAGT+GGG - chr4.1:46981810-46981829 None:intergenic 25.0%
! CAAAAAAAAAAAATTGCAGG+GGG - chr4.1:46982652-46982671 None:intergenic 25.0%
!!! AAACATTTTTTTTTACAGGG+AGG - chr4.1:46982680-46982699 None:intergenic 25.0%
!!! ACTGAACCATATTGATTTTT+GGG + chr4.1:46981810-46981829 MS.gene61651:CDS 25.0%
ATCTAATGATGACATACAAG+AGG + chr4.1:46982524-46982543 MS.gene61651:CDS 30.0%
CAAAAATCAATATGGTTCAG+TGG - chr4.1:46981811-46981830 None:intergenic 30.0%
CTTGAACCCAAAAATCAATA+TGG - chr4.1:46981819-46981838 None:intergenic 30.0%
GTCTTTATCTCGTGTAATAT+AGG + chr4.1:46982571-46982590 MS.gene61651:intron 30.0%
TACCTCCCTGTAAAAAAAAT+TGG - chr4.1:46982802-46982821 None:intergenic 30.0%
TCATTAGATTCACTTGAAAG+TGG - chr4.1:46982513-46982532 None:intergenic 30.0%
TGATAATTTCCATATTGCCA+TGG + chr4.1:46981750-46981769 MS.gene61651:CDS 30.0%
!! CGTTTTTGTTTGACCTATTA+GGG + chr4.1:46982764-46982783 MS.gene61651:intron 30.0%
!! GTTTTTGTTTGACCTATTAG+GGG + chr4.1:46982765-46982784 MS.gene61651:intron 30.0%
!! GTTTTTGTTTGGCCTATTAA+GGG - chr4.1:46982713-46982732 None:intergenic 30.0%
!! TCGTTTTTGTTTGACCTATT+AGG + chr4.1:46982763-46982782 MS.gene61651:intron 30.0%
!! TTTTTGTTTGACCTATTAGG+GGG + chr4.1:46982766-46982785 MS.gene61651:intron 30.0%
!! TTTTTGTTTGGCCTATTAAG+GGG - chr4.1:46982712-46982731 None:intergenic 30.0%
!!! AACCAATTTTTTTTACAGGG+AGG + chr4.1:46982797-46982816 MS.gene61651:intron 30.0%
!!! CACTGAACCATATTGATTTT+TGG + chr4.1:46981809-46981828 MS.gene61651:CDS 30.0%
AATAAGACAGTGTTCGAGAA+TGG - chr4.1:46981554-46981573 None:intergenic 35.0%
AGATATACGCTCGCAAAAAT+AGG - chr4.1:46981611-46981630 None:intergenic 35.0%
ATTCTCGAACACTGTCTTAT+TGG + chr4.1:46981553-46981572 MS.gene61651:CDS 35.0%
ATTGAATCTCACGATCAACA+AGG - chr4.1:46981585-46981604 None:intergenic 35.0%
CCTGTAATATAGGTCATTTC+CGG + chr4.1:46982622-46982641 MS.gene61651:intron 35.0%
CCTGTAATATAGGTCATTTC+CGG - chr4.1:46982641-46982622 None:intergenic 35.0%
CGGAAATGACCTATATTACA+GGG + chr4.1:46982851-46982870 MS.gene61651:intron 35.0%
CGTGTAATATAGGTCATTTC+CGG + chr4.1:46982581-46982600 MS.gene61651:intron 35.0%
GAAATGACCTATATTACAGG+AGG - chr4.1:46982872-46982853 None:intergenic 35.0%
GAAATGACCTATATTACAGG+GGG + chr4.1:46982853-46982872 MS.gene61651:intron 35.0%
GAGGTAAAGACCTATTAAAC+CGG - chr4.1:46982603-46982622 None:intergenic 35.0%
GGAAATGACCTATATTACAG+GGG + chr4.1:46982852-46982871 MS.gene61651:intron 35.0%
GTCTTTACCTCCTGTAATAT+AGG + chr4.1:46982612-46982631 MS.gene61651:intron 35.0%
GTGCTAGCTAAAAAAGGTAA+AGG + chr4.1:46982381-46982400 MS.gene61651:CDS 35.0%
TAGGTCATTTCCGGTTTAAT+AGG + chr4.1:46982590-46982609 MS.gene61651:intron 35.0%
TGGAAATTATCATACAACCG+TGG - chr4.1:46981742-46981761 None:intergenic 35.0%
! TCTTTTACCCCCTGTAATAT+AGG - chr4.1:46982863-46982882 None:intergenic 35.0%
! TTTGATTTACCCCCCTTAAT+AGG + chr4.1:46982698-46982717 MS.gene61651:intron 35.0%
!! GGTTTTTGTTTGGCCTATTA+AGG - chr4.1:46982714-46982733 None:intergenic 35.0%
!! TTTTGTTTGGCCTATTAAGG+GGG - chr4.1:46982711-46982730 None:intergenic 35.0%
!!! GATTTTTGGGTTCAAGTTCA+CGG + chr4.1:46981823-46981842 MS.gene61651:CDS 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG - chr4.1:46982780-46982799 None:intergenic 35.0%
AAAAATTGCAGGGGGAAAAC+CGG - chr4.1:46982644-46982663 None:intergenic 40.0%
AAAATTACAGGGGGGAAAAC+CGG + chr4.1:46982831-46982850 MS.gene61651:intron 40.0%
AAACCAGTTACGCAAAGGTT+TGG + chr4.1:46982156-46982175 MS.gene61651:CDS 40.0%
AATGGCAGCATTATCAGGTT+CGG - chr4.1:46981536-46981555 None:intergenic 40.0%
ACTTGAAACCAGTTACGCAA+AGG + chr4.1:46982151-46982170 MS.gene61651:CDS 40.0%
ATCCAACCAAAAGTAGGTCA+AGG + chr4.1:46981862-46981881 MS.gene61651:CDS 40.0%
CCATCATCAGAATCTAACTC+GGG - chr4.1:46982111-46982130 None:intergenic 40.0%
CCGGAAATGACCTATATTAC+AGG + chr4.1:46982850-46982869 MS.gene61651:intron 40.0%
CCGGAAATGACCTATATTAC+AGG - chr4.1:46982869-46982850 None:intergenic 40.0%
CCTTCCAACATTAACCTTGA+CGG + chr4.1:46981469-46981488 MS.gene61651:CDS 40.0%
CTTCTTCCAAGTCATCCTTT+GGG + chr4.1:46982468-46982487 MS.gene61651:CDS 40.0%
GAATAGGATCTTGCAACCAA+CGG - chr4.1:46982197-46982216 None:intergenic 40.0%
GATGACATACAAGAGGAAAG+TGG + chr4.1:46982531-46982550 MS.gene61651:intron 40.0%
GCCATCATCAGAATCTAACT+CGG - chr4.1:46982112-46982131 None:intergenic 40.0%
GGATTCATCCAACCAAAAGT+AGG + chr4.1:46981856-46981875 MS.gene61651:CDS 40.0%
GTTTGATGTGACATAGAAGC+AGG - chr4.1:46982219-46982238 None:intergenic 40.0%
TGCTTCCAAAATACATCTGC+TGG + chr4.1:46982413-46982432 MS.gene61651:CDS 40.0%
TGTGACATAGAAGCAGGAAT+AGG - chr4.1:46982213-46982232 None:intergenic 40.0%
TTCTTCCAAGTCATCCTTTG+GGG + chr4.1:46982469-46982488 MS.gene61651:CDS 40.0%
TTTGGTTGGATGAATCCGAA+AGG - chr4.1:46981853-46981872 None:intergenic 40.0%
TTTGTTTGGCCTATTAAGGG+GGG - chr4.1:46982710-46982729 None:intergenic 40.0%
! AATGCGGTGCTAGCTAAAAA+AGG + chr4.1:46982375-46982394 MS.gene61651:CDS 40.0%
! AATTGGGTGTGTTTTGTCAC+AGG + chr4.1:46982052-46982071 MS.gene61651:intron 40.0%
! AGAAGCCAGCAGATGTATTT+TGG - chr4.1:46982421-46982440 None:intergenic 40.0%
! AGGTTCGGTTTCAAGGTTAA+GGG - chr4.1:46981521-46981540 None:intergenic 40.0%
! AGTGGCTCGGATTTGAATAT+AGG + chr4.1:46982549-46982568 MS.gene61651:intron 40.0%
! CATTATCAGGTTCGGTTTCA+AGG - chr4.1:46981528-46981547 None:intergenic 40.0%
! GTAACGGTCAAACACTGATT+TGG - chr4.1:46981668-46981687 None:intergenic 40.0%
!! AGTAACAAAAGCACCGTTTG+TGG - chr4.1:46982013-46982032 None:intergenic 40.0%
!!! ACTCACCACTCTTTTTTCAG+TGG - chr4.1:46981985-46982004 None:intergenic 40.0%
!!! CTCACCACTCTTTTTTCAGT+GGG - chr4.1:46981984-46982003 None:intergenic 40.0%
ATCTTGCAACCAACGGTTTG+TGG - chr4.1:46982190-46982209 None:intergenic 45.0%
CAAGGCATAGGAAGCTTCTT+AGG + chr4.1:46981880-46981899 MS.gene61651:CDS 45.0%
CATACAAGAGGAAAGTGGCT+CGG + chr4.1:46982536-46982555 MS.gene61651:intron 45.0%
CCAAAAGTAGGTCAAGGCAT+AGG + chr4.1:46981868-46981887 MS.gene61651:CDS 45.0%
CCCGAGTTAGATTCTGATGA+TGG + chr4.1:46982108-46982127 MS.gene61651:CDS 45.0%
CCGTCAAGGTTAATGTTGGA+AGG - chr4.1:46981472-46981491 None:intergenic 45.0%
CCTTCTTCCAAGTCATCCTT+TGG + chr4.1:46982467-46982486 MS.gene61651:CDS 45.0%
CGATACGATCCATGGCAATA+TGG - chr4.1:46981762-46981781 None:intergenic 45.0%
CTGTCCCACTGAAAAAAGAG+TGG + chr4.1:46981977-46981996 MS.gene61651:CDS 45.0%
CTTTGTGGTGAAACTGGAAG+AGG - chr4.1:46981690-46981709 None:intergenic 45.0%
GAGACCGTCAAGGTTAATGT+TGG - chr4.1:46981476-46981495 None:intergenic 45.0%
GGTGAAACTGGAAGAGGTAA+CGG - chr4.1:46981684-46981703 None:intergenic 45.0%
GGTTAAACCCTCTTCAGACA+AGG - chr4.1:46981500-46981519 None:intergenic 45.0%
GTTCCAAACCTTTGCGTAAC+TGG - chr4.1:46982162-46982181 None:intergenic 45.0%
TATAGACCAAATCGCTGTGC+AGG + chr4.1:46982994-46983013 MS.gene61651:intron 45.0%
TCACATCAAACTCACCAAGC+AGG + chr4.1:46982228-46982247 MS.gene61651:CDS 45.0%
TCGAGAATGGCAGCATTATC+AGG - chr4.1:46981541-46981560 None:intergenic 45.0%
TTTAGCTAGCACCGCATTCT+GGG - chr4.1:46982373-46982392 None:intergenic 45.0%
! CAGGTTCGGTTTCAAGGTTA+AGG - chr4.1:46981522-46981541 None:intergenic 45.0%
! CCAAAGGATGACTTGGAAGA+AGG - chr4.1:46982470-46982489 None:intergenic 45.0%
! CCTATGCCTTGACCTACTTT+TGG - chr4.1:46981871-46981890 None:intergenic 45.0%
! TGTCACAGGTGTGGATTGAT+TGG + chr4.1:46982066-46982085 MS.gene61651:intron 45.0%
!! CATCGACTTTGTGGTGAAAC+TGG - chr4.1:46981696-46981715 None:intergenic 45.0%
!! TGCCTTGACCTACTTTTGGT+TGG - chr4.1:46981867-46981886 None:intergenic 45.0%
!! TTTTAGCTAGCACCGCATTC+TGG - chr4.1:46982374-46982393 None:intergenic 45.0%
!! TAAATAAAAAAAAAATTACA+GGG + chr4.1:46982820-46982839 MS.gene61651:intron 5.0%
!! TGAAAAAATAAAAATAAAAT+TGG - chr4.1:46982735-46982754 None:intergenic 5.0%
AACTGCAGCTACTGAAGGTG+TGG + chr4.1:46982269-46982288 MS.gene61651:CDS 50.0%
AGCGACTCTGACACGCATAT+TGG - chr4.1:46981947-46981966 None:intergenic 50.0%
ATATCTCGTGCATGGAAGCC+AGG + chr4.1:46981625-46981644 MS.gene61651:CDS 50.0%
CAAGTTCACGGTCTGCCTTT+CGG + chr4.1:46981835-46981854 MS.gene61651:CDS 50.0%
CACCTTCAGTAGCTGCAGTT+CGG - chr4.1:46982269-46982288 None:intergenic 50.0%
GCTGCACGTATCCTTGATGA+GGG + chr4.1:46981715-46981734 MS.gene61651:CDS 50.0%
GGTCTCACCTTGTCTGAAGA+GGG + chr4.1:46981490-46981509 MS.gene61651:CDS 50.0%
GGTTCAGTGGGACAAAGCTA+GGG - chr4.1:46981798-46981817 None:intergenic 50.0%
GTATCCTTGATGAGGGTCCA+CGG + chr4.1:46981722-46981741 MS.gene61651:CDS 50.0%
TCAAGTCAGCTCCCCAATTG+CGG - chr4.1:46982137-46982156 None:intergenic 50.0%
TCACTTGAAAGTGGAGCAGC+AGG - chr4.1:46982504-46982523 None:intergenic 50.0%
TGCTGCACGTATCCTTGATG+AGG + chr4.1:46981714-46981733 MS.gene61651:CDS 50.0%
TGGTTCAGTGGGACAAAGCT+AGG - chr4.1:46981799-46981818 None:intergenic 50.0%
! ACTGCAGCTACTGAAGGTGT+GGG + chr4.1:46982270-46982289 MS.gene61651:CDS 50.0%
! GCTAGTAGCTGAAGTACCTG+TGG - chr4.1:46982448-46982467 None:intergenic 50.0%
! GGTGTGTTTTGTCACAGGTG+TGG + chr4.1:46982057-46982076 MS.gene61651:intron 50.0%
!! CAAAAGCACCGTTTGTGGCA+CGG - chr4.1:46982008-46982027 None:intergenic 50.0%
!! TACGTGCAGCATCGACTTTG+TGG - chr4.1:46981705-46981724 None:intergenic 50.0%
!! TGATGATGGCGACCGCAATT+GGG + chr4.1:46982122-46982141 MS.gene61651:CDS 50.0%
ACAAAGCTAGGGACGACACC+AGG - chr4.1:46981787-46981806 None:intergenic 55.0%
ACAACCGTGGACCCTCATCA+AGG - chr4.1:46981729-46981748 None:intergenic 55.0%
ACCAGGAGCGATACGATCCA+TGG - chr4.1:46981770-46981789 None:intergenic 55.0%
CAACAGAAGCAGCACCTGCT+TGG - chr4.1:46982245-46982264 None:intergenic 55.0%
CAACCAACGGTTTGTGGCAG+CGG - chr4.1:46982184-46982203 None:intergenic 55.0%
CAGACAAGGTGAGACCGTCA+AGG - chr4.1:46981486-46981505 None:intergenic 55.0%
CCAAGCCCCAAAGGATGACT+TGG - chr4.1:46982477-46982496 None:intergenic 55.0%
CGGTCTCACCTTGTCTGAAG+AGG + chr4.1:46981489-46981508 MS.gene61651:CDS 55.0%
GCGAGCGTATATCTCGTGCA+TGG + chr4.1:46981617-46981636 MS.gene61651:CDS 55.0%
GTGATCGTGACCTTCTTGCC+TGG - chr4.1:46981646-46981665 None:intergenic 55.0%
TGCATGGAAGCCAGGCAAGA+AGG + chr4.1:46981633-46981652 MS.gene61651:CDS 55.0%
TGGTGAGTCCGTGCCACAAA+CGG + chr4.1:46981997-46982016 MS.gene61651:intron 55.0%
! GATGATGGCGACCGCAATTG+GGG + chr4.1:46982123-46982142 MS.gene61651:CDS 55.0%
! TGTGCTTGCTGCCCAGAATG+CGG + chr4.1:46982359-46982378 MS.gene61651:CDS 55.0%
!! CAAGCAGGTGCTGCTTCTGT+TGG + chr4.1:46982243-46982262 MS.gene61651:CDS 55.0%
!! CCAAGTCATCCTTTGGGGCT+TGG + chr4.1:46982474-46982493 MS.gene61651:CDS 55.0%
!! CTGATGATGGCGACCGCAAT+TGG + chr4.1:46982121-46982140 MS.gene61651:CDS 55.0%
!! CTGCTGGCTTCTTCTTCCAC+AGG + chr4.1:46982429-46982448 MS.gene61651:CDS 55.0%
GAACCGCTGCCACAAACCGT+TGG + chr4.1:46982178-46982197 MS.gene61651:CDS 60.0%
GCAGGTAAGCCAAGCCCCAA+AGG - chr4.1:46982486-46982505 None:intergenic 60.0%
GCCATGGATCGTATCGCTCC+TGG + chr4.1:46981766-46981785 MS.gene61651:CDS 60.0%
GGCCGAACTGCAGCTACTGA+AGG + chr4.1:46982264-46982283 MS.gene61651:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 46981463 46983021 46981463 ID=MS.gene61651
chr4.1 mRNA 46981463 46983021 46981463 ID=MS.gene61651.t1;Parent=MS.gene61651
chr4.1 exon 46981463 46981998 46981463 ID=MS.gene61651.t1.exon1;Parent=MS.gene61651.t1
chr4.1 CDS 46981463 46981998 46981463 ID=cds.MS.gene61651.t1;Parent=MS.gene61651.t1
chr4.1 exon 46982074 46982549 46982074 ID=MS.gene61651.t1.exon2;Parent=MS.gene61651.t1
chr4.1 CDS 46982074 46982549 46982074 ID=cds.MS.gene61651.t1;Parent=MS.gene61651.t1
chr4.1 exon 46982999 46983021 46982999 ID=MS.gene61651.t1.exon3;Parent=MS.gene61651.t1
chr4.1 CDS 46982999 46983021 46982999 ID=cds.MS.gene61651.t1;Parent=MS.gene61651.t1
Gene Sequence

>MS.gene61651

ATGGATCCTTCCAACATTAACCTTGACGGTCTCACCTTGTCTGAAGAGGGTTTAACCCTTAACCTTGAAACCGAACCTGATAATGCTGCCATTCTCGAACACTGTCTTATTGGTCGAGTCCTTGTTGATCGTGAGATTCAATTTGCCTATTTTTGCGAGCGTATATCTCGTGCATGGAAGCCAGGCAAGAAGGTCACGATCACCAAATCAGTGTTTGACCGTTACCTCTTCCAGTTTCACCACAAAGTCGATGCTGCACGTATCCTTGATGAGGGTCCACGGTTGTATGATAATTTCCATATTGCCATGGATCGTATCGCTCCTGGTGTCGTCCCTAGCTTTGTCCCACTGAACCATATTGATTTTTGGGTTCAAGTTCACGGTCTGCCTTTCGGATTCATCCAACCAAAAGTAGGTCAAGGCATAGGAAGCTTCTTAGGCACTTTCAAAGCATATGATAATCGCAACTCAATTCATAGTTCCAATATGCGTGTCAGAGTCGCTATTGATGTCACTGTCCCACTGAAAAAAGAGTGGTGAGTCCGTGCCACAAACGGTGCTTTTGTTACTGTTAACTTTAAATATGAAAAATTGGGTGTGTTTTGTCACAGGTGTGGATTGATTGGTCATACTGATAAAGTGTGTCCCGAGTTAGATTCTGATGATGGCGACCGCAATTGGGGAGCTGACTTGAAACCAGTTACGCAAAGGTTTGGAACCGCTGCCACAAACCGTTGGTTGCAAGATCCTATTCCTGCTTCTATGTCACATCAAACTCACCAAGCAGGTGCTGCTTCTGTTGGCCGAACTGCAGCTACTGAAGGTGTGGGAAATTTTGCAACTTTCAATGATCGTATGCTCACTCTTCAGATGCAACTTACTGCTATGCAACAAGATGTGCTTGCTGCCCAGAATGCGGTGCTAGCTAAAAAAGGTAAAGGTGCAAGTTCTGCTTCCAAAATACATCTGCTGGCTTCTTCTTCCACAGGTACTTCAGCTACTAGCCTTCTTCCAAGTCATCCTTTGGGGCTTGGCTTACCTGCTGCTCCACTTTCAAGTGAATCTAATGATGACATACAAGAGGAAAGTGGCTCGGATTTGAATATAGGTCTTTATCTCGTGTAATATAGGTCATTTCCGGTTTAATAGGTCTTTACCTCCTGTAATATAGGTCATTTCCGGTTTTCCCCCTGCAATTTTTTTTTTTTGATTTACCTCCCTGTAAAAAAAAATGTTTTGATTTACCCCCCTTAATAGGCCAAACAAAAACCAATTTTATTTTTATTTTTTCAAGAAATTTTCGTTTTTGTTTGACCTATTAGGGGGTAAATCAAAACCAATTTTTTTTACAGGGAGGTAAATAAAAAAAAAATTACAGGGGGGAAAACCGGAAATGACCTATATTACAGGGGGTAAAAGACCTATTAACCCAAAAATGAATAACTTAACAAAAGTTCTATAATTTGAAACTTACTTTTATATTTAATTTTAAAAACTCTATCATTTGACTTTAATAGATATTATGGGTAATTATAGACCAAATCGCTGTGCAGGAATGA
Protein sequence

>MS.gene61651.t1

MDPSNINLDGLTLSEEGLTLNLETEPDNAAILEHCLIGRVLVDREIQFAYFCERISRAWKPGKKVTITKSVFDRYLFQFHHKVDAARILDEGPRLYDNFHIAMDRIAPGVVPSFVPLNHIDFWVQVHGLPFGFIQPKVGQGIGSFLGTFKAYDNRNSIHSSNMRVRVAIDVTVPLKKEWCGLIGHTDKVCPELDSDDGDRNWGADLKPVTQRFGTAATNRWLQDPIPASMSHQTHQAGAASVGRTAATEGVGNFATFNDRMLTLQMQLTAMQQDVLAAQNAVLAKKGKGASSASKIHLLASSSTGTSATSLLPSHPLGLGLPAAPLSSESNDDIQEENQIAVQE