AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012019

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012019.t1 MTR_8g463110 42.105 133 63 2 131 263 1 119 3.72e-19 88.2
MS.gene012019.t1 MTR_3g053920 32.105 190 98 5 1 188 120 280 1.60e-16 78.2
MS.gene012019.t1 MTR_0010s0370 49.123 57 27 1 4 58 121 177 5.28e-12 63.9
MS.gene012019.t1 MTR_0010s0350 49.123 57 27 1 4 58 121 177 5.28e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012019.t1 AT2G17920 26.126 111 80 1 1 109 121 231 2.23e-11 63.9
MS.gene012019.t1 AT2G41590 28.283 99 69 1 1 97 121 219 3.56e-11 63.5
MS.gene012019.t1 AT5G36228 31.313 99 66 1 1 97 121 219 3.58e-11 63.5

Find 79 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTACAGATGTGGTCTTCT+TGG 0.172777 8.2:-28440751 MS.gene012019:CDS
CGACAAGGAAGTCCTGTTTA+AGG 0.278652 8.2:+28440458 None:intergenic
GACAATGGGGTGTCTAATTT+AGG 0.301312 8.2:-28440136 MS.gene012019:CDS
GCCTAACCTATCTAATTTGC+AGG 0.341949 8.2:-28440515 MS.gene012019:CDS
CAGGTACATGGCCTACCATT+TGG 0.344367 8.2:-28440982 MS.gene012019:CDS
AGGATGCTGGCTGCTAATAA+TGG 0.351544 8.2:-28440208 MS.gene012019:CDS
AGTTCAAAGCTTATGATATT+CGG 0.373561 8.2:-28440912 MS.gene012019:CDS
CTTCTTAATTCTTCTTCTGC+AGG 0.381849 8.2:-28440376 MS.gene012019:CDS
TCGGATGATGGCAGTGCATC+AGG 0.392285 8.2:-28440491 MS.gene012019:CDS
ATAGGTGTTACACGCCTTGC+TGG 0.404811 8.2:-28440283 MS.gene012019:CDS
TCTCCCCGCTCTCTTGTCCT+TGG 0.409261 8.2:-28440331 MS.gene012019:CDS
GGAGATCAATTCCCTTAAAC+AGG 0.414045 8.2:-28440470 MS.gene012019:CDS
TAGGAATGAATGTTAGCAAT+GGG 0.415486 8.2:-28440117 MS.gene012019:CDS
GAAGCTATTGTGTCTGCAAA+TGG 0.428063 8.2:-28440160 MS.gene012019:CDS
TCTGGTGGTGATTCTTCTGT+TGG 0.429475 8.2:-28440544 MS.gene012019:CDS
TTCTGCAGGTACCTTCCATC+AGG 0.434811 8.2:-28440362 MS.gene012019:CDS
GCAGCCCCTCTGACTATCAT+AGG 0.436271 8.2:-28440301 MS.gene012019:CDS
TAGGACACACTTTGTCTGTA+TGG 0.438093 8.2:+28440728 None:intergenic
TGTCTGCAAATGGGGACAAT+GGG 0.451752 8.2:-28440150 MS.gene012019:CDS
TACCTTGACCTACCCTCTGC+TGG 0.454509 8.2:+28440953 None:intergenic
GAAGAATTAAGAAGATGAAC+CGG 0.457930 8.2:+28440385 None:intergenic
CTCAACCGCTGAAGAAAGAA+TGG 0.464818 8.2:-28440840 MS.gene012019:CDS
TAAGCCAAGGACAAGAGAGC+GGG 0.471228 8.2:+28440327 None:intergenic
GACAAGGAAGTCCTGTTTAA+GGG 0.473079 8.2:+28440459 None:intergenic
AAGCTATTGTGTCTGCAAAT+GGG 0.475369 8.2:-28440159 MS.gene012019:CDS
CTTCCTTGTCGCTCAGAATA+CGG 0.477765 8.2:-28440446 MS.gene012019:CDS
TATGGAGTAGAACTCATTGC+AGG 0.478288 8.2:-28439153 MS.gene012019:CDS
TCCGACCCTGCAAATTAGAT+AGG 0.494282 8.2:+28440509 None:intergenic
CTCTGCTGGATAAAACCAAA+TGG 0.501806 8.2:+28440967 None:intergenic
TAATTTGCAGGGTCGGATGA+TGG 0.512280 8.2:-28440503 MS.gene012019:CDS
ATGATAGTCAGAGGGGCTGC+AGG 0.514811 8.2:+28440304 None:intergenic
CAGAGATGAGGAAAAGAAAG+AGG 0.519736 8.2:-28440228 MS.gene012019:CDS
AAATTGATCTCAAAGAGCTT+AGG 0.521745 8.2:+28440709 None:intergenic
TCAGGCGAACAACAAATGTC+TGG 0.522700 8.2:-28440562 MS.gene012019:intron
ACCTATCTAATTTGCAGGGT+CGG 0.525324 8.2:-28440510 MS.gene012019:CDS
GGAATGAATGTTAGCAATGG+GGG 0.525369 8.2:-28440115 MS.gene012019:CDS
GAGTTTGTGCAGCACCTACA+TGG 0.527869 8.2:+28439074 None:intergenic
AATACGGTGGTGGCTCGTAA+TGG 0.528618 8.2:-28440430 MS.gene012019:CDS
TGATGTCGAAGATGATGTTA+TGG 0.528847 8.2:-28440260 MS.gene012019:CDS
GCGCAGAAGATATGTCACTA+TGG 0.532193 8.2:-28439171 MS.gene012019:intron
GACATGTGGGTGCAGGTACA+TGG 0.537834 8.2:-28440994 MS.gene012019:CDS
GTGGTGGCTCGTAATGGTGC+TGG 0.538291 8.2:-28440424 MS.gene012019:CDS
ACCTTCCATTCTTTCTTCAG+CGG 0.541990 8.2:+28440835 None:intergenic
CTTGACTTTGATGCGCATGT+AGG 0.548537 8.2:+28440873 None:intergenic
ACCTGAATCACATGGACATG+TGG 0.558407 8.2:-28441008 None:intergenic
CTGGCTGCTAATAATGGAAA+GGG 0.558644 8.2:-28440202 MS.gene012019:CDS
GTGTCTGCAAATGGGGACAA+TGG 0.566417 8.2:-28440151 MS.gene012019:CDS
TTAGGAATGAATGTTAGCAA+TGG 0.567232 8.2:-28440118 MS.gene012019:CDS
CCCTGCAAATTAGATAGGTT+AGG 0.575997 8.2:+28440514 None:intergenic
TGTCGCTCAGAATACGGTGG+TGG 0.578047 8.2:-28440440 MS.gene012019:CDS
AGAAATAAACTCCTGATGGA+AGG 0.579648 8.2:+28440351 None:intergenic
TGGTTCATCCTCTATGACAC+CGG 0.582440 8.2:-28440404 MS.gene012019:CDS
GAGGAAAAGAAAGAGGATGC+TGG 0.587907 8.2:-28440221 MS.gene012019:CDS
GCTGGCTGCTAATAATGGAA+AGG 0.596180 8.2:-28440203 MS.gene012019:CDS
GCTGGATAAAACCAAATGGT+AGG 0.605643 8.2:+28440971 None:intergenic
TGGAAGGTTCGTGTGAATGA+CGG 0.608227 8.2:-28440820 MS.gene012019:CDS
TCACATGGACATGTGGGTGC+AGG 0.608394 8.2:-28441001 None:intergenic
ACCGCTGAAGAAAGAATGGA+AGG 0.610180 8.2:-28440836 MS.gene012019:CDS
GAGGGGCTGCAGGTAAGCCA+AGG 0.610291 8.2:+28440314 None:intergenic
AGCTATTGTGTCTGCAAATG+GGG 0.613366 8.2:-28440158 MS.gene012019:CDS
GGGGAGAAATAAACTCCTGA+TGG 0.616093 8.2:+28440347 None:intergenic
AGGAATGAATGTTAGCAATG+GGG 0.617577 8.2:-28440116 MS.gene012019:CDS
AAGATGAACCGGTGTCATAG+AGG 0.620529 8.2:+28440396 None:intergenic
GTAAGCCAAGGACAAGAGAG+CGG 0.626942 8.2:+28440326 None:intergenic
TAACACCTATGATAGTCAGA+GGG 0.627978 8.2:+28440296 None:intergenic
CTGTTGCGTGATAACCATGT+AGG 0.633555 8.2:-28439088 None:intergenic
ATCCAGCAGAGGGTAGGTCA+AGG 0.643113 8.2:-28440955 MS.gene012019:CDS
CCTAACCTATCTAATTTGCA+GGG 0.644193 8.2:-28440514 MS.gene012019:CDS
GTAACACCTATGATAGTCAG+AGG 0.644353 8.2:+28440295 None:intergenic
CCACCGTATTCTGAGCGACA+AGG 0.645171 8.2:+28440443 None:intergenic
AAGCCAAGGACAAGAGAGCG+GGG 0.654110 8.2:+28440328 None:intergenic
TGGATACCGAATCAGAGATG+AGG 0.654612 8.2:-28440240 MS.gene012019:CDS
CATCTTCGACATCACCAGCA+AGG 0.655768 8.2:+28440269 None:intergenic
GAATGAATGTTAGCAATGGG+GGG 0.656964 8.2:-28440114 MS.gene012019:CDS
GTCTGCAAATGGGGACAATG+GGG 0.665514 8.2:-28440149 MS.gene012019:CDS
GGCGAACAACAAATGTCTGG+TGG 0.668228 8.2:-28440559 MS.gene012019:CDS
CCTGAATCACATGGACATGT+GGG 0.695568 8.2:-28441007 None:intergenic
CCTTGTCGCTCAGAATACGG+TGG 0.714211 8.2:-28440443 MS.gene012019:CDS
AACACCTATGATAGTCAGAG+GGG 0.752311 8.2:+28440297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCAAATATGAAAAACT+CGG - chr8.2:28439305-28439324 MS.gene012019:intron 15.0%
!! AATCATAACCAAGAATATAA+CGG + chr8.2:28440224-28440243 None:intergenic 20.0%
!! AATTTATCTAATCAATATGC+AGG - chr8.2:28440447-28440466 MS.gene012019:CDS 20.0%
!! ATTGATTAGATAAATTACGA+AGG + chr8.2:28440443-28440462 None:intergenic 20.0%
!! GAAATTATTGAATCTATTCT+TGG - chr8.2:28440351-28440370 MS.gene012019:CDS 20.0%
!! TTCAATAGTTATATGATTGT+TGG + chr8.2:28440334-28440353 None:intergenic 20.0%
!!! GGAAAACTTCAATTTTATTT+CGG + chr8.2:28440203-28440222 None:intergenic 20.0%
!!! TTTTCATATTTGAAAAACAC+TGG + chr8.2:28439302-28439321 None:intergenic 20.0%
! AATTCAAGTATGAAAATGCA+TGG - chr8.2:28440807-28440826 MS.gene012019:CDS 25.0%
! AGTTCAAAGCTTATGATATT+CGG - chr8.2:28439174-28439193 MS.gene012019:intron 25.0%
! GATATCTGATATGAATGTAA+TGG - chr8.2:28439994-28440013 MS.gene012019:intron 25.0%
! TCACTTAAGAATAATAGATC+TGG + chr8.2:28440168-28440187 None:intergenic 25.0%
!! TTCATACTTGAATTTTGTCT+AGG + chr8.2:28440801-28440820 None:intergenic 25.0%
!!! AAACTTCAATTTTATTTCGG+TGG + chr8.2:28440200-28440219 None:intergenic 25.0%
!!! TTTGAGATCAATTTTGATGA+TGG - chr8.2:28439386-28439405 MS.gene012019:intron 25.0%
AAACTCTTAAACCAAGTGAA+CGG + chr8.2:28440618-28440637 None:intergenic 30.0%
AAATTGATCTCAAAGAGCTT+AGG + chr8.2:28439380-28439399 None:intergenic 30.0%
CAGATTAGGTACTTTAATTC+TGG + chr8.2:28440135-28440154 None:intergenic 30.0%
GAAGAATTAAGAAGATGAAC+CGG + chr8.2:28439704-28439723 None:intergenic 30.0%
GTTTGATGAGTATGTTGTAT+CGG - chr8.2:28440027-28440046 MS.gene012019:intron 30.0%
TAGGAATGAATGTTAGCAAT+GGG - chr8.2:28439969-28439988 MS.gene012019:intron 30.0%
TTAGGAATGAATGTTAGCAA+TGG - chr8.2:28439968-28439987 MS.gene012019:intron 30.0%
TTCACTTGGTTTAAGAGTTT+GGG - chr8.2:28440618-28440637 MS.gene012019:intron 30.0%
! AGATTTTCTAACATGGCATT+TGG + chr8.2:28440711-28440730 None:intergenic 30.0%
! ATACTTGAATTTTGTCTAGG+AGG + chr8.2:28440798-28440817 None:intergenic 30.0%
! CAGAATTAAAGTACCTAATC+TGG - chr8.2:28440133-28440152 MS.gene012019:CDS 30.0%
! GAAGTTTTCCGTTATATTCT+TGG - chr8.2:28440213-28440232 MS.gene012019:CDS 30.0%
!! ATGATATAGTCACTGATTCT+TGG - chr8.2:28440849-28440868 MS.gene012019:CDS 30.0%
!!! AATTTTATTTCGGTGGACTA+AGG + chr8.2:28440193-28440212 None:intergenic 30.0%
AAGCTATTGTGTCTGCAAAT+GGG - chr8.2:28439927-28439946 MS.gene012019:intron 35.0%
AAGTCATCAATAATGCACCA+AGG + chr8.2:28440474-28440493 None:intergenic 35.0%
ACAACATACTCATCAAACAG+TGG + chr8.2:28440025-28440044 None:intergenic 35.0%
AGAAATAAACTCCTGATGGA+AGG + chr8.2:28439738-28439757 None:intergenic 35.0%
AGGAATGAATGTTAGCAATG+GGG - chr8.2:28439970-28439989 MS.gene012019:intron 35.0%
CTTCTTAATTCTTCTTCTGC+AGG - chr8.2:28439710-28439729 MS.gene012019:intron 35.0%
CTTTGAACTCACCCAAAAAT+TGG + chr8.2:28439163-28439182 None:intergenic 35.0%
GTTCACTTGGTTTAAGAGTT+TGG - chr8.2:28440617-28440636 MS.gene012019:intron 35.0%
TAACACCTATGATAGTCAGA+GGG + chr8.2:28439793-28439812 None:intergenic 35.0%
TGATGACTTCAACGACATAA+TGG - chr8.2:28440485-28440504 MS.gene012019:CDS 35.0%
TGATGTCGAAGATGATGTTA+TGG - chr8.2:28439826-28439845 MS.gene012019:intron 35.0%
TTGAATCTATTCTTGGCACT+TGG - chr8.2:28440358-28440377 MS.gene012019:CDS 35.0%
! AAGATGAAACCAAGCATTGT+TGG + chr8.2:28440685-28440704 None:intergenic 35.0%
! ACTATCCAATTTTGTTGCAC+CGG - chr8.2:28440751-28440770 MS.gene012019:CDS 35.0%
! CCTAACCTATCTAATTTGCA+GGG - chr8.2:28439572-28439591 MS.gene012019:intron 35.0%
! TCTTTTCCTCATCTCTGATT+CGG + chr8.2:28439855-28439874 None:intergenic 35.0%
!! CAAGGTATCAGCCAATTTTT+GGG - chr8.2:28439149-28439168 MS.gene012019:CDS 35.0%
!! TCAAGGTATCAGCCAATTTT+TGG - chr8.2:28439148-28439167 MS.gene012019:CDS 35.0%
!!! AATTTTGATGATGGTGTGTG+AGG - chr8.2:28439395-28439414 MS.gene012019:intron 35.0%
!!! ATTTGGTTTTATCCAGCAGA+GGG - chr8.2:28439121-28439140 MS.gene012019:CDS 35.0%
!!! ATTTTGATGATGGTGTGTGA+GGG - chr8.2:28439396-28439415 MS.gene012019:intron 35.0%
AACACCTATGATAGTCAGAG+GGG + chr8.2:28439792-28439811 None:intergenic 40.0%
AAGCCGTTAATTAACCATCG+CGG + chr8.2:28440543-28440562 None:intergenic 40.0%
AATCTATTCTTGGCACTTGG+AGG - chr8.2:28440361-28440380 MS.gene012019:CDS 40.0%
ACCTTCCATTCTTTCTTCAG+CGG + chr8.2:28439254-28439273 None:intergenic 40.0%
AGCTACTAACAAATGGCAGT+AGG - chr8.2:28439463-28439482 MS.gene012019:intron 40.0%
AGCTATTGTGTCTGCAAATG+GGG - chr8.2:28439928-28439947 MS.gene012019:intron 40.0%
ATTCCATGCTACAACACTCA+TGG + chr8.2:28440082-28440101 None:intergenic 40.0%
CAACCGCGATGGTTAATTAA+CGG - chr8.2:28440537-28440556 MS.gene012019:CDS 40.0%
CAGAGATGAGGAAAAGAAAG+AGG - chr8.2:28439858-28439877 MS.gene012019:intron 40.0%
CCCTGCAAATTAGATAGGTT+AGG + chr8.2:28439575-28439594 None:intergenic 40.0%
CTAATCAATATGCAGGTCCT+TGG - chr8.2:28440454-28440473 MS.gene012019:CDS 40.0%
CTCTGCTGGATAAAACCAAA+TGG + chr8.2:28439122-28439141 None:intergenic 40.0%
GAAGCTATTGTGTCTGCAAA+TGG - chr8.2:28439926-28439945 MS.gene012019:intron 40.0%
GAATGAATGTTAGCAATGGG+GGG - chr8.2:28439972-28439991 MS.gene012019:intron 40.0%
GACAAGGAAGTCCTGTTTAA+GGG + chr8.2:28439630-28439649 None:intergenic 40.0%
GAGAATTCACATCAGGGATA+GGG + chr8.2:28439490-28439509 None:intergenic 40.0%
GCTGGATAAAACCAAATGGT+AGG + chr8.2:28439118-28439137 None:intergenic 40.0%
GGAATGAATGTTAGCAATGG+GGG - chr8.2:28439971-28439990 MS.gene012019:intron 40.0%
GGAGATCAATTCCCTTAAAC+AGG - chr8.2:28439616-28439635 MS.gene012019:intron 40.0%
GGTGCAACAAAATTGGATAG+TGG + chr8.2:28440752-28440771 None:intergenic 40.0%
GTAACACCTATGATAGTCAG+AGG + chr8.2:28439794-28439813 None:intergenic 40.0%
GTACTGCAGCTACTAACAAA+TGG - chr8.2:28439456-28439475 MS.gene012019:intron 40.0%
GTGACTATATCATGAAAGCC+TGG + chr8.2:28440842-28440861 None:intergenic 40.0%
TAGGACACACTTTGTCTGTA+TGG + chr8.2:28439361-28439380 None:intergenic 40.0%
TATGGAGTAGAACTCATTGC+AGG - chr8.2:28440933-28440952 MS.gene012019:CDS 40.0%
! AAACCAAGCATTGTTGGCAA+GGG + chr8.2:28440679-28440698 None:intergenic 40.0%
! ACCTATCTAATTTGCAGGGT+CGG - chr8.2:28439576-28439595 MS.gene012019:intron 40.0%
! ATTGGATAGTGGTATGAAGC+CGG + chr8.2:28440741-28440760 None:intergenic 40.0%
! CTGGCTGCTAATAATGGAAA+GGG - chr8.2:28439884-28439903 MS.gene012019:intron 40.0%
! CTTGAATTTTGTCTAGGAGG+AGG + chr8.2:28440795-28440814 None:intergenic 40.0%
! GACAATGGGGTGTCTAATTT+AGG - chr8.2:28439950-28439969 MS.gene012019:intron 40.0%
! GCCTAACCTATCTAATTTGC+AGG - chr8.2:28439571-28439590 MS.gene012019:intron 40.0%
! GGCAGCAAGATTTTCTAACA+TGG + chr8.2:28440718-28440737 None:intergenic 40.0%
!!! CATTTGGTTTTATCCAGCAG+AGG - chr8.2:28439120-28439139 MS.gene012019:CDS 40.0%
!!! GGTGTTTTTTGCTACAGATG+TGG - chr8.2:28439326-28439345 MS.gene012019:intron 40.0%
ACCGCTGAAGAAAGAATGGA+AGG - chr8.2:28439250-28439269 MS.gene012019:intron 45.0%
ATCCCTGATGTGAATTCTCG+TGG - chr8.2:28439491-28439510 MS.gene012019:intron 45.0%
ATGGATGACAGTGAGAAGAG+AGG - chr8.2:28440504-28440523 MS.gene012019:CDS 45.0%
ATTACGAAGGAAGTTCCACG+CGG + chr8.2:28440430-28440449 None:intergenic 45.0%
CGACAAGGAAGTCCTGTTTA+AGG + chr8.2:28439631-28439650 None:intergenic 45.0%
CGAGAATTCACATCAGGGAT+AGG + chr8.2:28439491-28439510 None:intergenic 45.0%
CTCAACCGCTGAAGAAAGAA+TGG - chr8.2:28439246-28439265 MS.gene012019:intron 45.0%
CTTCCTTGTCGCTCAGAATA+CGG - chr8.2:28439640-28439659 MS.gene012019:intron 45.0%
CTTGACTTTGATGCGCATGT+AGG + chr8.2:28439216-28439235 None:intergenic 45.0%
GAGGAAAAGAAAGAGGATGC+TGG - chr8.2:28439865-28439884 MS.gene012019:intron 45.0%
GCGCAGAAGATATGTCACTA+TGG - chr8.2:28440915-28440934 MS.gene012019:CDS 45.0%
GGGGAGAAATAAACTCCTGA+TGG + chr8.2:28439742-28439761 None:intergenic 45.0%
GTGTTGTAGCATGGAATTGC+CGG - chr8.2:28440085-28440104 MS.gene012019:intron 45.0%
GTTAGAAAATCTTGCTGCCC+CGG - chr8.2:28440719-28440738 MS.gene012019:CDS 45.0%
TAGGACCGGTGCAACAAAAT+TGG + chr8.2:28440759-28440778 None:intergenic 45.0%
TCAGGCGAACAACAAATGTC+TGG - chr8.2:28439524-28439543 MS.gene012019:intron 45.0%
TCCGACCCTGCAAATTAGAT+AGG + chr8.2:28439580-28439599 None:intergenic 45.0%
TCTCGTGGCAGTACTAATTC+AGG - chr8.2:28439506-28439525 MS.gene012019:intron 45.0%
TCTGCGCATGCAGTAAGTTT+AGG + chr8.2:28440902-28440921 None:intergenic 45.0%
TGCCACGAGAATTCACATCA+GGG + chr8.2:28439496-28439515 None:intergenic 45.0%
TGCTACAGATGTGGTCTTCT+TGG - chr8.2:28439335-28439354 MS.gene012019:intron 45.0%
TGGAAGGTTCGTGTGAATGA+CGG - chr8.2:28439266-28439285 MS.gene012019:intron 45.0%
TGGATACCGAATCAGAGATG+AGG - chr8.2:28439846-28439865 MS.gene012019:intron 45.0%
TGGTTCATCCTCTATGACAC+CGG - chr8.2:28439682-28439701 MS.gene012019:intron 45.0%
TGTCTGCAAATGGGGACAAT+GGG - chr8.2:28439936-28439955 MS.gene012019:intron 45.0%
TGTTGTAGCATGGAATTGCC+GGG - chr8.2:28440086-28440105 MS.gene012019:intron 45.0%
! AAGCATTGTTGGCAAGGGAT+CGG + chr8.2:28440674-28440693 None:intergenic 45.0%
! AGGATGCTGGCTGCTAATAA+TGG - chr8.2:28439878-28439897 MS.gene012019:intron 45.0%
! GAAACCAAGCATTGTTGGCA+AGG + chr8.2:28440680-28440699 None:intergenic 45.0%
! GATGAGTATGTTGTATCGGC+AGG - chr8.2:28440031-28440050 MS.gene012019:intron 45.0%
! GCTGGCTGCTAATAATGGAA+AGG - chr8.2:28439883-28439902 MS.gene012019:intron 45.0%
! TAATTTGCAGGGTCGGATGA+TGG - chr8.2:28439583-28439602 MS.gene012019:intron 45.0%
! TCTGGTGGTGATTCTTCTGT+TGG - chr8.2:28439542-28439561 MS.gene012019:intron 45.0%
! TTGGATAGTGGTATGAAGCC+GGG + chr8.2:28440740-28440759 None:intergenic 45.0%
!! AAGATGAACCGGTGTCATAG+AGG + chr8.2:28439693-28439712 None:intergenic 45.0%
!! CGTCAAGCTGTTTTAGACTC+GGG - chr8.2:28440564-28440583 MS.gene012019:intron 45.0%
!! GCGTCAAGCTGTTTTAGACT+CGG - chr8.2:28440563-28440582 MS.gene012019:intron 45.0%
!! TAGAAGGGTATCCGTTCACT+TGG - chr8.2:28440604-28440623 MS.gene012019:intron 45.0%
!! TGCTTTTCTGTTGACTGCAC+CGG - chr8.2:28440246-28440265 MS.gene012019:CDS 45.0%
AAACCAGTAGCTCGTCGTGT+TGG - chr8.2:28439434-28439453 MS.gene012019:intron 50.0%
AATGCATGGCAGCTTGAACC+AGG - chr8.2:28440821-28440840 MS.gene012019:CDS 50.0%
AGTCTCTCTTCAACAGCTCG+TGG + chr8.2:28440648-28440667 None:intergenic 50.0%
CAGGTACATGGCCTACCATT+TGG - chr8.2:28439104-28439123 MS.gene012019:CDS 50.0%
CATCTTCGACATCACCAGCA+AGG + chr8.2:28439820-28439839 None:intergenic 50.0%
CATGTCTACGTTCCCCATTG+GGG + chr8.2:28440407-28440426 None:intergenic 50.0%
CTGCCACGAGAATTCACATC+AGG + chr8.2:28439497-28439516 None:intergenic 50.0%
GATCCCTTGCCAACAATGCT+TGG - chr8.2:28440673-28440692 MS.gene012019:intron 50.0%
GCATGTCTACGTTCCCCATT+GGG + chr8.2:28440408-28440427 None:intergenic 50.0%
GGCGAACAACAAATGTCTGG+TGG - chr8.2:28439527-28439546 MS.gene012019:intron 50.0%
GTAAGCCAAGGACAAGAGAG+CGG + chr8.2:28439763-28439782 None:intergenic 50.0%
GTCTGCAAATGGGGACAATG+GGG - chr8.2:28439937-28439956 MS.gene012019:intron 50.0%
GTGAACGGATACCCTTCTAG+AGG + chr8.2:28440603-28440622 None:intergenic 50.0%
GTGTCTGCAAATGGGGACAA+TGG - chr8.2:28439935-28439954 MS.gene012019:intron 50.0%
GTTACTACGGGTACCCCAAT+GGG - chr8.2:28440391-28440410 MS.gene012019:CDS 50.0%
GTTGTAGCATGGAATTGCCG+GGG - chr8.2:28440087-28440106 MS.gene012019:intron 50.0%
TAAGCCAAGGACAAGAGAGC+GGG + chr8.2:28439762-28439781 None:intergenic 50.0%
TTACTACGGGTACCCCAATG+GGG - chr8.2:28440392-28440411 MS.gene012019:CDS 50.0%
TTCTGCAGGTACCTTCCATC+AGG - chr8.2:28439724-28439743 MS.gene012019:intron 50.0%
TTGTCAGACGTGCCTCTAGA+AGG - chr8.2:28440588-28440607 MS.gene012019:intron 50.0%
! AATACGGTGGTGGCTCGTAA+TGG - chr8.2:28439656-28439675 MS.gene012019:intron 50.0%
! ATAGGTGTTACACGCCTTGC+TGG - chr8.2:28439803-28439822 MS.gene012019:intron 50.0%
! ATTGGGGTACCCGTAGTAAC+CGG + chr8.2:28440391-28440410 None:intergenic 50.0%
! CTGGATTGAAGCGCCAGATT+AGG + chr8.2:28440149-28440168 None:intergenic 50.0%
! GGTCCATGAGTGTTGTAGCA+TGG - chr8.2:28440076-28440095 MS.gene012019:intron 50.0%
! TATGTTGTATCGGCAGGTCC+TGG - chr8.2:28440037-28440056 MS.gene012019:intron 50.0%
! TGGAGGCTTACCGGTTACTA+CGG - chr8.2:28440378-28440397 MS.gene012019:CDS 50.0%
! TGGATAGTGGTATGAAGCCG+GGG + chr8.2:28440739-28440758 None:intergenic 50.0%
! TGTCAGACGTGCCTCTAGAA+GGG - chr8.2:28440589-28440608 MS.gene012019:intron 50.0%
!! GCGGGGGTCTTGCTATATTT+TGG - chr8.2:28440274-28440293 MS.gene012019:CDS 50.0%
!! GGTTTTATCCAGCAGAGGGT+AGG - chr8.2:28439125-28439144 MS.gene012019:CDS 50.0%
!! TGATGATGGTGTGTGAGGGT+GGG - chr8.2:28439400-28439419 MS.gene012019:intron 50.0%
!! TTGATGATGGTGTGTGAGGG+TGG - chr8.2:28439399-28439418 MS.gene012019:intron 50.0%
AAGCCAAGGACAAGAGAGCG+GGG + chr8.2:28439761-28439780 None:intergenic 55.0%
ATCCAGCAGAGGGTAGGTCA+AGG - chr8.2:28439131-28439150 MS.gene012019:CDS 55.0%
ATGATAGTCAGAGGGGCTGC+AGG + chr8.2:28439785-28439804 None:intergenic 55.0%
CCACCGTATTCTGAGCGACA+AGG + chr8.2:28439646-28439665 None:intergenic 55.0%
CCTTGTCGCTCAGAATACGG+TGG - chr8.2:28439643-28439662 MS.gene012019:intron 55.0%
CTTGGCACTTGGAGGCTTAC+CGG - chr8.2:28440369-28440388 MS.gene012019:CDS 55.0%
GACATGTGGGTGCAGGTACA+TGG - chr8.2:28439092-28439111 MS.gene012019:CDS 55.0%
GAGCACGAGGCATATAGGAC+CGG + chr8.2:28440773-28440792 None:intergenic 55.0%
GCAGCCCCTCTGACTATCAT+AGG - chr8.2:28439785-28439804 MS.gene012019:intron 55.0%
GCATGGAATTGCCGGGGTTT+AGG - chr8.2:28440093-28440112 MS.gene012019:intron 55.0%
GGCATGTCTACGTTCCCCAT+TGG + chr8.2:28440409-28440428 None:intergenic 55.0%
GGTTACTACGGGTACCCCAA+TGG - chr8.2:28440390-28440409 MS.gene012019:CDS 55.0%
GTACCAACACGACGAGCTAC+TGG + chr8.2:28439440-28439459 None:intergenic 55.0%
TACCTTGACCTACCCTCTGC+TGG + chr8.2:28439136-28439155 None:intergenic 55.0%
TGTCGCTCAGAATACGGTGG+TGG - chr8.2:28439646-28439665 MS.gene012019:intron 55.0%
! AGGAGGAGCACGAGGCATAT+AGG + chr8.2:28440778-28440797 None:intergenic 55.0%
! GATGATGGTGTGTGAGGGTG+GGG - chr8.2:28439401-28439420 MS.gene012019:intron 55.0%
! GGAGGCTTACCGGTTACTAC+GGG - chr8.2:28440379-28440398 MS.gene012019:CDS 55.0%
! GTTGACTGCACCGGTAGAAG+CGG - chr8.2:28440255-28440274 MS.gene012019:CDS 55.0%
! TCGGATGATGGCAGTGCATC+AGG - chr8.2:28439595-28439614 MS.gene012019:intron 55.0%
! TTGACTGCACCGGTAGAAGC+GGG - chr8.2:28440256-28440275 MS.gene012019:CDS 55.0%
!! CACGACGAGCTACTGGTTTG+AGG + chr8.2:28439433-28439452 None:intergenic 55.0%
!! CGAGCTACTGGTTTGAGGTC+AGG + chr8.2:28439428-28439447 None:intergenic 55.0%
AGCAAGACCCCCGCTTCTAC+CGG + chr8.2:28440268-28440287 None:intergenic 60.0%
ATCGGCAGGTCCTGGAACTC+AGG - chr8.2:28440045-28440064 MS.gene012019:intron 60.0%
CAACACTCATGGACCCCAGC+AGG + chr8.2:28440071-28440090 None:intergenic 60.0%
CACTCGAGCGACCTAAACCC+CGG + chr8.2:28440107-28440126 None:intergenic 60.0%
GACGTGCTCTACAACCGCGA+TGG - chr8.2:28440526-28440545 MS.gene012019:CDS 60.0%
GTGGTGGCTCGTAATGGTGC+TGG - chr8.2:28439662-28439681 MS.gene012019:intron 60.0%
TCCTGGAACTCAGGCCTGCT+GGG - chr8.2:28440054-28440073 MS.gene012019:intron 60.0%
TCTCCCCGCTCTCTTGTCCT+TGG - chr8.2:28439755-28439774 MS.gene012019:intron 60.0%
! TGACTGCACCGGTAGAAGCG+GGG - chr8.2:28440257-28440276 MS.gene012019:CDS 60.0%
! TGTCTAGGAGGAGGAGCACG+AGG + chr8.2:28440786-28440805 None:intergenic 60.0%
CCTGGAACTCAGGCCTGCTG+GGG - chr8.2:28440055-28440074 MS.gene012019:intron 65.0%
GAGGGGCTGCAGGTAAGCCA+AGG + chr8.2:28439775-28439794 None:intergenic 65.0%
GGGAACGTAGACATGCCGCG+TGG - chr8.2:28440412-28440431 MS.gene012019:CDS 65.0%
GTCCTGGAACTCAGGCCTGC+TGG - chr8.2:28440053-28440072 MS.gene012019:intron 65.0%
! GACTGCACCGGTAGAAGCGG+GGG - chr8.2:28440258-28440277 MS.gene012019:CDS 65.0%
CCCCAGCAGGCCTGAGTTCC+AGG + chr8.2:28440058-28440077 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 28439089 28441019 28439089 ID=MS.gene012019
chr8.2 mRNA 28439089 28441019 28439089 ID=MS.gene012019.t1;Parent=MS.gene012019
chr8.2 exon 28440701 28441019 28440701 ID=MS.gene012019.t1.exon1;Parent=MS.gene012019.t1
chr8.2 CDS 28440701 28441019 28440701 ID=cds.MS.gene012019.t1;Parent=MS.gene012019.t1
chr8.2 exon 28440099 28440580 28440099 ID=MS.gene012019.t1.exon2;Parent=MS.gene012019.t1
chr8.2 CDS 28440099 28440580 28440099 ID=cds.MS.gene012019.t1;Parent=MS.gene012019.t1
chr8.2 exon 28439089 28439187 28439089 ID=MS.gene012019.t1.exon3;Parent=MS.gene012019.t1
chr8.2 CDS 28439089 28439187 28439089 ID=cds.MS.gene012019.t1;Parent=MS.gene012019.t1
Gene Sequence

>MS.gene012019

ATGGACATGTGGGTGCAGGTACATGGCCTACCATTTGGTTTTATCCAGCAGAGGGTAGGTCAAGGTATCAGCCAATTTTTGGGTGAGTTCAAAGCTTATGATATTCGGAATACTGTGCACAGCTCCTACATGCGCATCAAAGTCAAGCTGAATGTTACTCAACCGCTGAAGAAAGAATGGAAGGTTCGTGTGAATGACGGAAACTATGTACCAGTGTTTTTCAAATATGAAAAACTCGGTGTTTTTTGCTACAGATGTGGTCTTCTTGGCCATACAGACAAAGTGTGTCCTAAGCTCTTTGAGATCAATTTTGATGATGGTGTGTGAGGGTGGGGACCTGACCTCAAACCAGTAGCTCGTCGTGTTGGTACTGCAGCTACTAACAAATGGCAGTAGGACCCTATCCCTGATGTGAATTCTCGTGGCAGTACTAATTCAGGCGAACAACAAATGTCTGGTGGTGATTCTTCTGTTGGTGTTGCGCCTAACCTATCTAATTTGCAGGGTCGGATGATGGCAGTGCATCAGGAGATCAATTCCCTTAAACAGGACTTCCTTGTCGCTCAGAATACGGTGGTGGCTCGTAATGGTGCTGGTTCATCCTCTATGACACCGGTTCATCTTCTTAATTCTTCTTCTGCAGGTACCTTCCATCAGGAGTTTATTTCTCCCCGCTCTCTTGTCCTTGGCTTACCTGCAGCCCCTCTGACTATCATAGGTGTTACACGCCTTGCTGGTGATGTCGAAGATGATGTTATGGATACCGAATCAGAGATGAGGAAAAGAAAGAGGATGCTGGCTGCTAATAATGGAAAGGGTGTAGCTGTTGAATGTGCTGAAGCTATTGTGTCTGCAAATGGGGACAATGGGGTGTCTAATTTAGGAATGAATGTTAGCAATGGGGGGATATCTGATATGAATGTAATGGATAATCCACTGTTTGATGAGTATGTTGTATCGGCAGGTCCTGGAACTCAGGCCTGCTGGGGTCCATGAGTGTTGTAGCATGGAATTGCCGGGGTTTAGGTCGCTCGAGTGTGATTCCAGAATTAAAGTACCTAATCTGGCGCTTCAATCCAGATCTATTATTCTTAAGTGAGACCTTAGTCCACCGAAATAAAATTGAAGTTTTCCGTTATATTCTTGGTTATGATTCTTGCTTTTCTGTTGACTGCACCGGTAGAAGCGGGGGTCTTGCTATATTTTGGCGTTCTTCTTTAAATTGCAATCTTATAAATTACTCCAACAATCATATAACTATTGAAATTATTGAATCTATTCTTGGCACTTGGAGGCTTACCGGTTACTACGGGTACCCCAATGGGGAACGTAGACATGCCGCGTGGAACTTCCTTCGTAATTTATCTAATCAATATGCAGGTCCTTGGTGCATTATTGATGACTTCAACGACATAATGGATGACAGTGAGAAGAGAGGACGTGCTCTACAACCGCGATGGTTAATTAACGGCTTGCGTCAAGCTGTTTTAGACTCGGGTTTGTCAGACGTGCCTCTAGAAGGGTATCCGTTCACTTGGTTTAAGAGTTTGGGAACTCCACGAGCTGTTGAAGAGAGACTAGACCGATCCCTTGCCAACAATGCTTGGTTTCATCTTTTTCCAAATGCCATGTTAGAAAATCTTGCTGCCCCGGCTTCATACCACTATCCAATTTTGTTGCACCGGTCCTATATGCCTCGTGCTCCTCCTCCTAGACAAAATTCAAGTATGAAAATGCATGGCAGCTTGAACCAGGCTTTCATGATATAGTCACTGATTCTTGGAACATGAATTTATCTCATCTCATCATTCCTAAACTTACTGCATGCGCAGAAGATATGTCACTATGGAGTAGAACTCATTGCAGGAAACTTAAAACTGACATTGAAGAGTGCTCACGACAACTGCAGCTGTTGCGTGATAACCATGTAG
Protein sequence

>MS.gene012019.t1

MDMWVQVHGLPFGFIQQRVGQGISQFLGEFKAYDIRNTVHSSYMRIKVKLNVTQPLKKEWKVRVNDGNYVPVFFKYEKLGVFCYRCGLLGHTDKVCPKLFEINFDDGEQQMSGGDSSVGVAPNLSNLQGRMMAVHQEINSLKQDFLVAQNTVVARNGAGSSSMTPVHLLNSSSAGTFHQEFISPRSLVLGLPAAPLTIIGVTRLAGDVEDDVMDTESEMRKRKRMLAANNGKGVAVECAEAIVSANGDNGVSNLGMNVSNGGISDMNKICHYGVELIAGNLKLTLKSAHDNCSCCVITM