AlfalfaGEDB | MS.gene05231

M. sativa cultivar XinJiangDaYe / MS.gene05231

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05231.t1	XP_024639426.1	89.8	265	27	0	65	329	1	265	3.60E-144	521.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05231.t1	A0A396HU84	89.6	259	27	0	71	329	1	259	2.3e-140	508.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene72520	MS.gene05231	PPI
MS.gene05231	MS.gene027169	PPI
MS.gene013193	MS.gene05231	PPI
MS.gene05231	MS.gene81712	PPI
MS.gene05231	MS.gene019177	PPI
MS.gene05231	MS.gene05436	PPI
MS.gene05231	MS.gene038623	PPI
MS.gene05231	MS.gene005120	PPI
MS.gene05231	MS.gene055560	PPI
MS.gene05231	MS.gene024297	PPI
MS.gene05231	MS.gene35202	PPI
MS.gene05231	MS.gene057934	PPI
MS.gene05231	MS.gene37537	PPI
MS.gene05231	MS.gene069786	PPI
MS.gene05231	MS.gene42010	PPI
MS.gene05231	MS.gene025158	PPI
MS.gene05231	MS.gene049512	PPI
MS.gene05231	MS.gene27329	PPI
MS.gene83979	MS.gene05231	PPI
MS.gene05231	MS.gene87529	PPI
MS.gene05231	MS.gene41393	PPI
MS.gene05231	MS.gene97410	PPI
MS.gene05231	MS.gene010656	PPI
MS.gene57044	MS.gene05231	PPI
MS.gene05231	MS.gene016632	PPI
MS.gene05231	MS.gene018474	PPI
MS.gene47792	MS.gene05231	PPI
MS.gene038676	MS.gene05231	PPI
MS.gene05231	MS.gene40469	PPI
MS.gene05231	MS.gene96163	PPI
MS.gene05231	MS.gene65623	PPI
MS.gene05231	MS.gene012748	PPI
MS.gene05231	MS.gene66651	PPI
MS.gene042651	MS.gene05231	PPI
MS.gene93396	MS.gene05231	PPI
MS.gene05231	MS.gene02896	PPI
MS.gene05231	MS.gene71588	PPI
MS.gene05231	MS.gene61452	PPI
MS.gene05231	MS.gene03149	PPI
MS.gene010656	MS.gene05231	PPI
MS.gene05231	MS.gene037049	PPI
MS.gene05231	MS.gene042614	PPI
MS.gene05231	MS.gene026680	PPI
MS.gene05231	MS.gene61499	PPI
MS.gene050679	MS.gene05231	PPI
MS.gene05231	MS.gene051449	PPI
MS.gene20246	MS.gene05231	PPI
MS.gene05231	MS.gene019165	PPI
MS.gene05231	MS.gene81194	PPI
MS.gene05231	MS.gene060773	PPI
MS.gene05231	MS.gene038394	PPI
MS.gene05231	MS.gene09589	PPI
MS.gene05231	MS.gene019001	PPI
MS.gene061850	MS.gene05231	PPI
MS.gene05231	MS.gene021965	PPI
MS.gene05231	MS.gene53904	PPI
MS.gene05231	MS.gene24070	PPI
MS.gene05231	MS.gene83772	PPI
MS.gene05231	MS.gene73261	PPI
MS.gene05231	MS.gene059736	PPI
MS.gene05231	MS.gene85538	PPI
MS.gene05231	MS.gene025675	PPI
MS.gene05231	MS.gene26844	PPI
MS.gene05231	MS.gene055546	PPI
MS.gene042559	MS.gene05231	PPI
MS.gene05231	MS.gene71637	PPI
MS.gene05231	MS.gene55209	PPI
MS.gene05231	MS.gene96174	PPI
MS.gene05231	MS.gene074257	PPI
MS.gene05231	MS.gene04749	PPI
MS.gene05231	MS.gene073573	PPI
MS.gene05231	MS.gene28718	PPI
MS.gene69490	MS.gene05231	PPI
MS.gene015248	MS.gene05231	PPI
MS.gene05231	MS.gene63743	PPI
MS.gene34812	MS.gene05231	PPI
MS.gene05231	MS.gene055223	PPI
MS.gene05231	MS.gene068629	PPI
MS.gene05231	MS.gene004417	PPI
MS.gene05231	MS.gene009781	PPI
MS.gene08106	MS.gene05231	PPI
MS.gene05231	MS.gene031967	PPI
MS.gene067340	MS.gene05231	PPI
MS.gene05231	MS.gene21066	PPI
MS.gene05231	MS.gene009213	PPI
MS.gene05231	MS.gene013890	PPI
MS.gene05231	MS.gene98127	PPI
MS.gene05231	MS.gene003264	PPI
MS.gene05231	MS.gene054652	PPI
MS.gene05231	MS.gene000634	PPI
MS.gene038623	MS.gene05231	PPI
MS.gene05357	MS.gene05231	PPI
MS.gene05231	MS.gene86849	PPI
MS.gene062067	MS.gene05231	PPI
MS.gene05231	MS.gene80362	PPI
MS.gene05231	MS.gene49057	PPI
MS.gene05231	MS.gene89815	PPI
MS.gene05231	MS.gene048088	PPI
MS.gene05231	MS.gene86868	PPI
MS.gene05231	MS.gene058889	PPI
MS.gene09589	MS.gene05231	PPI
MS.gene05231	MS.gene034643	PPI
MS.gene05231	MS.gene60059	PPI
MS.gene05231	MS.gene012889	PPI
MS.gene05231	MS.gene34463	PPI
MS.gene05231	MS.gene038757	PPI
MS.gene05231	MS.gene000506	PPI
MS.gene05231	MS.gene51804	PPI
MS.gene05231	MS.gene013644	PPI
MS.gene04749	MS.gene05231	PPI
MS.gene42903	MS.gene05231	PPI
MS.gene05231	MS.gene061579	PPI
MS.gene048747	MS.gene05231	PPI
MS.gene05231	MS.gene04970	PPI
MS.gene22200	MS.gene05231	PPI
MS.gene05231	MS.gene26038	PPI
MS.gene05231	MS.gene060788	PPI
MS.gene05231	MS.gene43924	PPI
MS.gene05231	MS.gene41643	PPI
MS.gene94654	MS.gene05231	PPI
MS.gene05231	MS.gene022929	PPI
MS.gene05231	MS.gene85295	PPI
MS.gene05231	MS.gene26433	PPI
MS.gene05231	MS.gene007022	PPI
MS.gene031967	MS.gene05231	PPI
MS.gene05231	MS.gene69584	PPI
MS.gene05231	MS.gene52349	PPI
MS.gene063214	MS.gene05231	PPI
MS.gene97410	MS.gene05231	PPI
MS.gene83772	MS.gene05231	PPI
MS.gene32415	MS.gene05231	PPI
MS.gene05231	MS.gene021647	PPI
MS.gene05231	MS.gene047954	PPI
MS.gene05231	MS.gene65471	PPI
MS.gene035626	MS.gene05231	PPI
MS.gene05231	MS.gene071784	PPI
MS.gene05231	MS.gene048342	PPI
MS.gene05231	MS.gene069624	PPI
MS.gene05231	MS.gene060386	PPI
MS.gene63762	MS.gene05231	PPI
MS.gene05231	MS.gene83979	PPI
MS.gene05231	MS.gene073582	PPI
MS.gene30208	MS.gene05231	PPI
MS.gene69495	MS.gene05231	PPI
MS.gene05231	MS.gene63762	PPI
MS.gene05231	MS.gene016719	PPI
MS.gene05231	MS.gene048072	PPI
MS.gene05231	MS.gene028714	PPI
MS.gene05231	MS.gene88393	PPI
MS.gene05231	MS.gene042027	PPI
MS.gene05231	MS.gene048706	PPI
MS.gene032311	MS.gene05231	PPI
MS.gene05231	MS.gene69586	PPI
MS.gene05231	MS.gene45083	PPI
MS.gene030090	MS.gene05231	PPI
MS.gene95561	MS.gene05231	PPI
MS.gene05231	MS.gene049561	PPI
MS.gene05231	MS.gene008658	PPI
MS.gene05231	MS.gene47792	PPI
MS.gene018196	MS.gene05231	PPI
MS.gene05231	MS.gene63150	PPI
MS.gene05231	MS.gene03936	PPI
MS.gene57019	MS.gene05231	PPI
MS.gene05231	MS.gene43393	PPI
MS.gene05231	MS.gene000629	PPI
MS.gene05231	MS.gene072852	PPI
MS.gene05231	MS.gene65474	PPI
MS.gene021968	MS.gene05231	PPI
MS.gene05231	MS.gene08708	PPI
MS.gene05231	MS.gene04134	PPI
MS.gene05231	MS.gene06164	PPI
MS.gene05231	MS.gene50392	PPI
MS.gene05231	MS.gene86275	PPI
MS.gene60059	MS.gene05231	PPI
MS.gene05231	MS.gene22730	PPI
MS.gene05231	MS.gene032299	PPI
MS.gene05231	MS.gene017586	PPI
MS.gene05231	MS.gene038098	PPI
MS.gene43393	MS.gene05231	PPI
MS.gene05231	MS.gene037634	PPI
MS.gene05231	MS.gene057828	PPI
MS.gene05231	MS.gene071320	PPI
MS.gene05231	MS.gene08606	PPI
MS.gene05231	MS.gene012019	PPI
MS.gene008275	MS.gene05231	PPI
MS.gene05231	MS.gene000640	PPI
MS.gene05231	MS.gene061850	PPI
MS.gene05231	MS.gene98756	PPI
MS.gene05231	MS.gene39956	PPI
MS.gene048072	MS.gene05231	PPI
MS.gene05231	MS.gene028716	PPI
MS.gene05231	MS.gene74639	PPI
MS.gene05231	MS.gene071133	PPI
MS.gene05231	MS.gene64130	PPI
MS.gene05231	MS.gene60266	PPI
MS.gene05231	MS.gene98467	PPI
MS.gene05231	MS.gene024522	PPI
MS.gene05231	MS.gene54051	PPI
MS.gene05231	MS.gene22111	PPI
MS.gene05231	MS.gene046089	PPI
MS.gene05436	MS.gene05231	PPI
MS.gene05231	MS.gene049610	PPI
MS.gene05231	MS.gene010290	PPI
MS.gene05231	MS.gene37955	PPI
MS.gene05231	MS.gene92517	PPI
MS.gene05231	MS.gene34197	PPI
MS.gene05231	MS.gene36069	PPI
MS.gene05231	MS.gene045875	PPI
MS.gene05231	MS.gene010890	PPI
MS.gene05231	MS.gene84790	PPI
MS.gene05231	MS.gene71675	PPI
MS.gene05231	MS.gene055683	PPI
MS.gene05231	MS.gene05319	PPI
MS.gene055683	MS.gene05231	PPI
MS.gene05231	MS.gene039692	PPI
MS.gene05231	MS.gene032311	PPI
MS.gene05231	MS.gene55682	PPI
MS.gene05231	MS.gene002590	PPI
MS.gene05231	MS.gene41979	PPI
MS.gene66651	MS.gene05231	PPI
MS.gene96109	MS.gene05231	PPI
MS.gene05231	MS.gene008927	PPI
MS.gene051449	MS.gene05231	PPI
MS.gene05231	MS.gene98616	PPI
MS.gene05231	MS.gene23521	PPI
MS.gene05231	MS.gene77990	PPI
MS.gene05231	MS.gene65546	PPI
MS.gene05231	MS.gene062511	PPI
MS.gene05231	MS.gene008861	PPI
MS.gene05231	MS.gene027994	PPI
MS.gene05231	MS.gene059269	PPI
MS.gene05231	MS.gene045614	PPI
MS.gene05231	MS.gene024841	PPI
MS.gene05231	MS.gene064966	PPI
MS.gene30136	MS.gene05231	PPI
MS.gene05231	MS.gene76420	PPI
MS.gene05231	MS.gene46353	PPI
MS.gene05231	MS.gene40828	PPI
MS.gene016102	MS.gene05231	PPI
MS.gene46941	MS.gene05231	PPI
MS.gene96541	MS.gene05231	PPI
MS.gene05231	MS.gene51799	PPI
MS.gene05231	MS.gene98471	PPI
MS.gene05231	MS.gene062067	PPI
MS.gene05231	MS.gene61651	PPI
MS.gene05231	MS.gene013676	PPI
MS.gene05231	MS.gene29406	PPI
MS.gene46948	MS.gene05231	PPI
MS.gene025873	MS.gene05231	PPI
MS.gene012019	MS.gene05231	PPI
MS.gene05231	MS.gene09023	PPI
MS.gene05358	MS.gene05231	PPI
MS.gene05231	MS.gene054508	PPI
MS.gene05231	MS.gene025987	PPI
MS.gene05231	MS.gene95960	PPI
MS.gene05231	MS.gene053373	PPI
MS.gene05231	MS.gene043140	PPI
MS.gene05231	MS.gene041264	PPI
MS.gene05231	MS.gene35175	PPI
MS.gene065627	MS.gene05231	PPI
MS.gene05231	MS.gene44083	PPI
MS.gene000629	MS.gene05231	PPI
MS.gene05231	MS.gene29321	PPI
MS.gene05231	MS.gene018745	PPI
MS.gene05231	MS.gene025401	PPI
MS.gene05231	MS.gene57044	PPI
MS.gene05231	MS.gene015093	PPI
MS.gene05319	MS.gene05231	PPI
MS.gene038098	MS.gene05231	PPI
MS.gene56991	MS.gene05231	PPI
MS.gene05231	MS.gene24278	PPI
MS.gene05231	MS.gene000379	PPI
MS.gene05231	MS.gene34618	PPI
MS.gene055223	MS.gene05231	PPI
MS.gene05231	MS.gene59460	PPI
MS.gene28545	MS.gene05231	PPI
MS.gene05231	MS.gene66650	PPI
MS.gene05231	MS.gene009121	PPI
MS.gene05231	MS.gene08713	PPI
MS.gene05231	MS.gene70932	PPI
MS.gene05231	MS.gene09517	PPI
MS.gene05231	MS.gene87845	PPI
MS.gene05231	MS.gene024405	PPI
MS.gene064966	MS.gene05231	PPI
MS.gene05231	MS.gene85308	PPI
MS.gene05231	MS.gene62249	PPI
MS.gene05231	MS.gene58419	PPI
MS.gene05231	MS.gene64178	PPI
MS.gene98616	MS.gene05231	PPI
MS.gene05231	MS.gene053747	PPI
MS.gene036423	MS.gene05231	PPI
MS.gene05231	MS.gene021555	PPI
MS.gene05231	MS.gene44144	PPI
MS.gene05231	MS.gene05576	PPI
MS.gene05231	MS.gene060718	PPI
MS.gene05231	MS.gene044729	PPI
MS.gene05231	MS.gene60390	PPI
MS.gene05231	MS.gene48477	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05231.t1	MTR_6g033215	43.243	185	105	0	12	196	110	294	2.35e-56	185
MS.gene05231.t1	MTR_0010s0370	37.647	170	104	1	7	174	8	177	5.48e-37	131
MS.gene05231.t1	MTR_0010s0350	37.647	170	104	1	7	174	8	177	5.48e-37	131
MS.gene05231.t1	MTR_6g053230	37.107	159	64	1	27	185	49	171	5.05e-25	105
MS.gene05231.t1	MTR_3g053920	30.588	170	91	3	14	183	19	161	5.00e-24	99.8
MS.gene05231.t1	MTR_8g468010	55.882	68	30	0	112	179	1	68	1.97e-21	89.0
MS.gene05231.t1	MTR_2g061500	33.333	111	74	0	14	124	19	129	2.39e-21	88.6
MS.gene05231.t1	MTR_3g024240	36.885	122	75	1	78	197	54	175	1.15e-20	88.2
MS.gene05231.t1	MTR_1g032270	43.836	73	41	0	96	168	2	74	5.49e-17	77.0
MS.gene05231.t1	MTR_4g103530	28.261	138	94	2	50	184	1	136	9.78e-16	74.7
MS.gene05231.t1	MTR_1g036720	38.961	77	47	0	31	107	34	110	1.46e-14	69.3
MS.gene05231.t1	MTR_7g089990	29.231	130	81	2	101	228	86	206	4.07e-12	65.1
MS.gene05231.t1	MTR_6g053790	27.711	166	90	5	132	292	34	174	4.38e-12	66.2
MS.gene05231.t1	MTR_7g011710	26.829	123	83	3	50	170	1	118	2.22e-11	61.6
MS.gene05231.t1	MTR_4g075740	40.000	60	36	0	53	112	61	120	8.86e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05231.t1	AT2G13450	21.622	185	143	2	31	215	39	221	6.62e-15	75.5
MS.gene05231.t1	AT3G31430	24.324	185	136	1	29	213	132	312	1.66e-14	73.6
MS.gene05231.t1	AT3G57587	22.727	154	113	2	64	215	72	221	1.48e-13	71.2
MS.gene05231.t1	AT2G17920	21.463	205	151	4	19	215	19	221	7.81e-13	68.6
MS.gene05231.t1	AT2G41590	20.652	184	140	2	31	212	39	218	7.35e-12	66.2
MS.gene05231.t1	AT5G18636	19.786	187	144	2	28	212	36	218	2.07e-11	64.7
MS.gene05231.t1	AT5G25200	19.786	187	144	2	28	212	36	218	2.19e-11	64.7
MS.gene05231.t1	AT2G02103	19.786	187	144	2	28	212	36	218	2.72e-11	64.7

Find 97 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATAATTCCATTGAGTCTA+TGG	0.207359	6.2:+77779529	None:intergenic
TTGATAGCTACCCTCTAATT+TGG	0.212488	6.2:-77779589	MS.gene05231:CDS
GTTCTTTAGCTATGCTGATA+TGG	0.273773	6.2:-77779642	MS.gene05231:CDS
GGAATAGGGGAAGGAGAGTT+TGG	0.275936	6.2:-77779106	MS.gene05231:CDS
CGCTGCTCAACCGGTTGGTT+CGG	0.281042	6.2:-77778919	MS.gene05231:CDS
TATGATCATGAGAACAACTA+TGG	0.291990	6.2:-77779422	MS.gene05231:CDS
TTCTCAATCATTCCTCCTTC+TGG	0.297653	6.2:+77779149	None:intergenic
CCATAGACTCAATGGAATTA+TGG	0.325596	6.2:-77779529	MS.gene05231:CDS
TGAAGCCGAAGGGGAGATTA+TGG	0.332526	6.2:+77779498	None:intergenic
TGTTACGAATGTGGTTTGAT+AGG	0.358266	6.2:-77779266	MS.gene05231:CDS
TTCTTCAAAGGTTCTGTAAC+CGG	0.362287	6.2:+77779362	None:intergenic
TGGAGGATGGTGCTGTTATA+AGG	0.365760	6.2:-77779322	MS.gene05231:CDS
TTGAGCAGAGTTTGAGGATT+GGG	0.367979	6.2:+77779856	None:intergenic
AGACAGTAGTCAAACGTATC+AGG	0.383957	6.2:+77779791	None:intergenic
GCTGCTCAACCGGTTGGTTC+GGG	0.393964	6.2:-77778918	MS.gene05231:CDS
GTTGCATGATGGTAGGAATA+GGG	0.395437	6.2:-77779120	MS.gene05231:CDS
CACACCAGACGCTGCTCAAC+CGG	0.396925	6.2:-77778928	MS.gene05231:CDS
AACGGACAGGTTTGGTGGTT+AGG	0.398533	6.2:+77779756	None:intergenic
GGTTGCATGATGGTAGGAAT+AGG	0.403724	6.2:-77779121	MS.gene05231:CDS
GTAACCTTGCTCCAAATTAG+AGG	0.404545	6.2:+77779578	None:intergenic
GAATTTCCGTTATGAGAAAC+CGG	0.404718	6.2:-77779297	MS.gene05231:CDS
TCAGGTTGACATTACCTTGA+TGG	0.412908	6.2:-77779687	MS.gene05231:CDS
AAACTCGTATGTCGACTCTA+TGG	0.415488	6.2:-77779721	MS.gene05231:CDS
CAAACAACCAAGGTCCTTGT+TGG	0.427763	6.2:+77779609	None:intergenic
AACTGGAACATGAAACGGTT+TGG	0.434533	6.2:+77779662	None:intergenic
TGTGGAAGGACAACAGCAAT+GGG	0.435056	6.2:-77779201	MS.gene05231:CDS
ATGAGAACAACTATGGGAAC+TGG	0.437973	6.2:-77779415	MS.gene05231:CDS
AATTTCCCGGTTTCTCATAA+CGG	0.445801	6.2:+77779291	None:intergenic
TATCAGCATAGCTAAAGAAC+TGG	0.450298	6.2:+77779645	None:intergenic
AATGGGTGCGCTTCGATGTT+GGG	0.454613	6.2:-77778962	MS.gene05231:CDS
CCCTACACGACTCTAATGAA+TGG	0.469607	6.2:-77778980	MS.gene05231:CDS
CACTATGCCCATAGACTCAA+TGG	0.470706	6.2:-77779537	MS.gene05231:CDS
AATTCAAAGCTCTTCTTCAA+AGG	0.471184	6.2:+77779350	None:intergenic
TTAGGCATGAACCATCTGTA+TGG	0.471262	6.2:+77779243	None:intergenic
ACGTTTGACTACTGTCTTGT+TGG	0.474338	6.2:-77779785	MS.gene05231:CDS
GGAGGATGGTGCTGTTATAA+GGG	0.476915	6.2:-77779321	MS.gene05231:CDS
CGGCTTCATGACTGAGACGA+TGG	0.477746	6.2:-77779483	MS.gene05231:CDS
CAAGTCCATAATCTCCCCTT+CGG	0.480491	6.2:-77779503	MS.gene05231:CDS
AGAATTGTTCCCGAACCAAC+CGG	0.483752	6.2:+77778909	None:intergenic
TTAAAATTGTGAAAACGGAC+AGG	0.485342	6.2:+77779743	None:intergenic
TGAGACGATGGGAATTCTGC+TGG	0.491039	6.2:-77779471	MS.gene05231:CDS
GAGCTTTGAATTTGTGTTGG+AGG	0.500306	6.2:-77779339	MS.gene05231:CDS
TAAAGAACTGGAACATGAAA+CGG	0.501455	6.2:+77779657	None:intergenic
GAAGAGCTTTGAATTTGTGT+TGG	0.503025	6.2:-77779342	MS.gene05231:CDS
ATTTGGAGCAAGGTTACTGA+TGG	0.507984	6.2:-77779572	MS.gene05231:CDS
ATTGTGAAAACGGACAGGTT+TGG	0.511118	6.2:+77779748	None:intergenic
TTTGAATTTGTGTTGGAGGA+TGG	0.511654	6.2:-77779335	MS.gene05231:CDS
GAGTCTATGGGCATAGTGAA+TGG	0.511703	6.2:+77779542	None:intergenic
CCTACACGACTCTAATGAAT+GGG	0.511968	6.2:-77778979	MS.gene05231:CDS
TCTATGGCAGCCTAAGCATC+AGG	0.513233	6.2:-77779705	MS.gene05231:CDS
ATTCTGCTGGGTAATCATGT+AGG	0.515667	6.2:-77779458	MS.gene05231:CDS
GAATGGGTGCGCTTCGATGT+TGG	0.518655	6.2:-77778963	MS.gene05231:CDS
GGCTTCATGACTGAGACGAT+GGG	0.519564	6.2:-77779482	MS.gene05231:CDS
GAAACGGTTTGGTTCCATCA+AGG	0.523942	6.2:+77779673	None:intergenic
CATAATTCCATTGAGTCTAT+GGG	0.529160	6.2:+77779530	None:intergenic
CCAACCGGTTGAGCAGCGTC+TGG	0.530582	6.2:+77778924	None:intergenic
TTGTGGAAGGACAACAGCAA+TGG	0.534414	6.2:-77779202	MS.gene05231:CDS
TTTGAGCAGAGTTTGAGGAT+TGG	0.536790	6.2:+77779855	None:intergenic
TCTGATTATGTTGGACCAGA+AGG	0.543826	6.2:-77779164	MS.gene05231:CDS
GTGAAAACGGACAGGTTTGG+TGG	0.548358	6.2:+77779751	None:intergenic
GTATCAGGAGTGTAGGGTGT+TGG	0.548384	6.2:+77779806	None:intergenic
TTCTGCTGGGTAATCATGTA+GGG	0.556103	6.2:-77779457	MS.gene05231:CDS
TTGCATGATGGTAGGAATAG+GGG	0.557746	6.2:-77779119	MS.gene05231:CDS
GTCAAACGTATCAGGAGTGT+AGG	0.562495	6.2:+77779799	None:intergenic
CCCATTCATTAGAGTCGTGT+AGG	0.574790	6.2:+77778979	None:intergenic
GTCTCAGTCATGAAGCCGAA+GGG	0.577163	6.2:+77779488	None:intergenic
CTACCCTCTAATTTGGAGCA+AGG	0.577584	6.2:-77779582	MS.gene05231:CDS
GTAATGTCAACCTGATGCTT+AGG	0.580576	6.2:+77779695	None:intergenic
CAGGAGTGTAGGGTGTTGGA+TGG	0.585542	6.2:+77779810	None:intergenic
ATGATCATGAGAACAACTAT+GGG	0.585783	6.2:-77779421	MS.gene05231:CDS
AGATGTGGTTACTAGAGGAC+TGG	0.588803	6.2:-77779024	MS.gene05231:CDS
GAGTTATCCAACAAGGACCT+TGG	0.593632	6.2:-77779616	MS.gene05231:CDS
AGTTAAAATTGGAAGAGATG+TGG	0.595966	6.2:-77779039	MS.gene05231:CDS
TCAAACGTATCAGGAGTGTA+GGG	0.597215	6.2:+77779800	None:intergenic
CCATTCATTAGAGTCGTGTA+GGG	0.599556	6.2:+77778980	None:intergenic
GAGACGATGGGAATTCTGCT+GGG	0.604091	6.2:-77779470	MS.gene05231:CDS
CGTCTCAGTCATGAAGCCGA+AGG	0.608173	6.2:+77779487	None:intergenic
TAACCTTGCTCCAAATTAGA+GGG	0.608564	6.2:+77779579	None:intergenic
ACATAATCAGACCTCAGATA+CGG	0.617955	6.2:+77779176	None:intergenic
GGAAGAGATGTGGTTACTAG+AGG	0.618859	6.2:-77779029	MS.gene05231:CDS
GAGGAATGATTGAGAATCCG+TGG	0.624102	6.2:-77779142	MS.gene05231:CDS
AGCAATGGGGACCGTATCTG+AGG	0.625340	6.2:-77779187	MS.gene05231:CDS
TATCTGAGGTCTGATTATGT+TGG	0.625809	6.2:-77779173	MS.gene05231:CDS
CCAGACGCTGCTCAACCGGT+TGG	0.631370	6.2:-77778924	MS.gene05231:CDS
GAGGCTGAGAGTATCTCTGC+CGG	0.632739	6.2:-77779381	MS.gene05231:CDS
GAGAATCCGTGGTTGCATGA+TGG	0.635499	6.2:-77779131	MS.gene05231:CDS
GATTATGTTGGACCAGAAGG+AGG	0.637278	6.2:-77779161	MS.gene05231:CDS
ATCCGTGGTTGCATGATGGT+AGG	0.639254	6.2:-77779127	MS.gene05231:CDS
GGTTTGATAGGCCATACAGA+TGG	0.649457	6.2:-77779254	MS.gene05231:CDS
AATTTCCGTTATGAGAAACC+GGG	0.650488	6.2:-77779296	MS.gene05231:CDS
TTCCTACCATCATGCAACCA+CGG	0.653639	6.2:+77779125	None:intergenic
TCTCAGTCATGAAGCCGAAG+GGG	0.661623	6.2:+77779489	None:intergenic
ATGATGGTAGGAATAGGGGA+AGG	0.670532	6.2:-77779115	MS.gene05231:CDS
GGAACTGGAGAAGATACATG+AGG	0.718382	6.2:-77779400	MS.gene05231:CDS
ATGGAACGAGTTATCCAACA+AGG	0.722162	6.2:-77779623	MS.gene05231:CDS
GGGTAGCTATCAAACAACCA+AGG	0.725612	6.2:+77779599	None:intergenic
GTGGAAGGACAACAGCAATG+GGG	0.734059	6.2:-77779200	MS.gene05231:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GAGTTTTAAAATTGTGAAAA+CGG	+	chr6.2:77779018-77779037	None:intergenic	20.0%
!!	AACCTTTCTCAAATCTTTTT+AGG	+	chr6.2:77779531-77779550	None:intergenic	25.0%
!!!	TTTTAACTCTACCGAAAAAA+CGG	+	chr6.2:77779702-77779721	None:intergenic	25.0%
!!!	TTTTTCGGTAGAGTTAAAAT+TGG	-	chr6.2:77779703-77779722	MS.gene05231:CDS	25.0%
	AATTCAAAGCTCTTCTTCAA+AGG	+	chr6.2:77779406-77779425	None:intergenic	30.0%
	AGTTAAAATTGGAAGAGATG+TGG	-	chr6.2:77779714-77779733	MS.gene05231:CDS	30.0%
	ATGATCATGAGAACAACTAT+GGG	-	chr6.2:77779332-77779351	MS.gene05231:CDS	30.0%
	CATAATTCCATTGAGTCTAT+GGG	+	chr6.2:77779226-77779245	None:intergenic	30.0%
	CATTCGTAACAAAAATTTCC+CGG	+	chr6.2:77779478-77779497	None:intergenic	30.0%
	TAAAGAACTGGAACATGAAA+CGG	+	chr6.2:77779099-77779118	None:intergenic	30.0%
	TATGATCATGAGAACAACTA+TGG	-	chr6.2:77779331-77779350	MS.gene05231:CDS	30.0%
	TGCCTAAAAAGATTTGAGAA+AGG	-	chr6.2:77779526-77779545	MS.gene05231:CDS	30.0%
	TTAAAATTGTGAAAACGGAC+AGG	+	chr6.2:77779013-77779032	None:intergenic	30.0%
!	AAGATTTGAGAAAGGTTTTG+TGG	-	chr6.2:77779534-77779553	MS.gene05231:CDS	30.0%
!	GGAAATTTTTGTTACGAATG+TGG	-	chr6.2:77779478-77779497	MS.gene05231:CDS	30.0%
!!!	ATGGCTATTTTTAGTGGAAT+TGG	+	chr6.2:77778927-77778946	None:intergenic	30.0%
	AATTTCCCGGTTTCTCATAA+CGG	+	chr6.2:77779465-77779484	None:intergenic	35.0%
	AATTTCCGTTATGAGAAACC+GGG	-	chr6.2:77779457-77779476	MS.gene05231:CDS	35.0%
	ACATAATCAGACCTCAGATA+CGG	+	chr6.2:77779580-77779599	None:intergenic	35.0%
	CCATAATTCCATTGAGTCTA+TGG	+	chr6.2:77779227-77779246	None:intergenic	35.0%
	CCATAGACTCAATGGAATTA+TGG	-	chr6.2:77779224-77779243	MS.gene05231:CDS	35.0%
	GAATTTCCGTTATGAGAAAC+CGG	-	chr6.2:77779456-77779475	MS.gene05231:CDS	35.0%
	GTTCTTTAGCTATGCTGATA+TGG	-	chr6.2:77779111-77779130	MS.gene05231:CDS	35.0%
	TAACCTTGCTCCAAATTAGA+GGG	+	chr6.2:77779177-77779196	None:intergenic	35.0%
	TATCAGCATAGCTAAAGAAC+TGG	+	chr6.2:77779111-77779130	None:intergenic	35.0%
	TGTTACGAATGTGGTTTGAT+AGG	-	chr6.2:77779487-77779506	MS.gene05231:CDS	35.0%
	TTCTTCAAAGGTTCTGTAAC+CGG	+	chr6.2:77779394-77779413	None:intergenic	35.0%
	TTTGAATTTGTGTTGGAGGA+TGG	-	chr6.2:77779418-77779437	MS.gene05231:CDS	35.0%
!	TATCTGAGGTCTGATTATGT+TGG	-	chr6.2:77779580-77779599	MS.gene05231:CDS	35.0%
!	TTGATAGCTACCCTCTAATT+TGG	-	chr6.2:77779164-77779183	MS.gene05231:CDS	35.0%
!!	CTGGTCTTTTATAGACACAT+TGG	-	chr6.2:77779748-77779767	MS.gene05231:CDS	35.0%
!!	GAAGAGCTTTGAATTTGTGT+TGG	-	chr6.2:77779411-77779430	MS.gene05231:CDS	35.0%
!!	TTTGAGAAAGGTTTTGTGGA+AGG	-	chr6.2:77779538-77779557	MS.gene05231:CDS	35.0%
!!!	TCTAACCTACACCGTTTTTT+CGG	-	chr6.2:77779688-77779707	MS.gene05231:CDS	35.0%
!!!	TGTTGGATGGCTATTTTTAG+TGG	+	chr6.2:77778933-77778952	None:intergenic	35.0%
	AACTGGAACATGAAACGGTT+TGG	+	chr6.2:77779094-77779113	None:intergenic	40.0%
	ACGTTTGACTACTGTCTTGT+TGG	-	chr6.2:77778968-77778987	MS.gene05231:CDS	40.0%
	AGACAGTAGTCAAACGTATC+AGG	+	chr6.2:77778965-77778984	None:intergenic	40.0%
	ATGAGAACAACTATGGGAAC+TGG	-	chr6.2:77779338-77779357	MS.gene05231:CDS	40.0%
	ATGGAACGAGTTATCCAACA+AGG	-	chr6.2:77779130-77779149	MS.gene05231:CDS	40.0%
	ATTCTGCTGGGTAATCATGT+AGG	-	chr6.2:77779295-77779314	MS.gene05231:CDS	40.0%
	ATTGTGAAAACGGACAGGTT+TGG	+	chr6.2:77779008-77779027	None:intergenic	40.0%
	CCATTCATTAGAGTCGTGTA+GGG	+	chr6.2:77779776-77779795	None:intergenic	40.0%
	CCTACACGACTCTAATGAAT+GGG	-	chr6.2:77779774-77779793	MS.gene05231:CDS	40.0%
	GTAACCTTGCTCCAAATTAG+AGG	+	chr6.2:77779178-77779197	None:intergenic	40.0%
	GTAATGTCAACCTGATGCTT+AGG	+	chr6.2:77779061-77779080	None:intergenic	40.0%
	GTTGCATGATGGTAGGAATA+GGG	-	chr6.2:77779633-77779652	MS.gene05231:CDS	40.0%
	TCTGATTATGTTGGACCAGA+AGG	-	chr6.2:77779589-77779608	MS.gene05231:CDS	40.0%
	TTAGGCATGAACCATCTGTA+TGG	+	chr6.2:77779513-77779532	None:intergenic	40.0%
	TTCTCAATCATTCCTCCTTC+TGG	+	chr6.2:77779607-77779626	None:intergenic	40.0%
	TTCTGCTGGGTAATCATGTA+GGG	-	chr6.2:77779296-77779315	MS.gene05231:CDS	40.0%
	TTGAGCAGAGTTTGAGGATT+GGG	+	chr6.2:77778900-77778919	None:intergenic	40.0%
	TTGCATGATGGTAGGAATAG+GGG	-	chr6.2:77779634-77779653	MS.gene05231:CDS	40.0%
	TTTGAGCAGAGTTTGAGGAT+TGG	+	chr6.2:77778901-77778920	None:intergenic	40.0%
!	ATTTGGAGCAAGGTTACTGA+TGG	-	chr6.2:77779181-77779200	MS.gene05231:CDS	40.0%
!	TCAAACGTATCAGGAGTGTA+GGG	+	chr6.2:77778956-77778975	None:intergenic	40.0%
!!	AAACTCGTATGTCGACTCTA+TGG	-	chr6.2:77779032-77779051	MS.gene05231:CDS	40.0%
!!	GAGCTTTGAATTTGTGTTGG+AGG	-	chr6.2:77779414-77779433	MS.gene05231:CDS	40.0%
!!	GGTATTTTGAGCAGAGTTTG+AGG	+	chr6.2:77778906-77778925	None:intergenic	40.0%
!!	TCAGGTTGACATTACCTTGA+TGG	-	chr6.2:77779066-77779085	MS.gene05231:CDS	40.0%
	AGAATTGTTCCCGAACCAAC+CGG	+	chr6.2:77779847-77779866	None:intergenic	45.0%
	AGATGTGGTTACTAGAGGAC+TGG	-	chr6.2:77779729-77779748	MS.gene05231:CDS	45.0%
	ATGATGGTAGGAATAGGGGA+AGG	-	chr6.2:77779638-77779657	MS.gene05231:CDS	45.0%
	CAAACAACCAAGGTCCTTGT+TGG	+	chr6.2:77779147-77779166	None:intergenic	45.0%
	CAAGTCCATAATCTCCCCTT+CGG	-	chr6.2:77779250-77779269	MS.gene05231:CDS	45.0%
	CACTATGCCCATAGACTCAA+TGG	-	chr6.2:77779216-77779235	MS.gene05231:CDS	45.0%
	CCCATTCATTAGAGTCGTGT+AGG	+	chr6.2:77779777-77779796	None:intergenic	45.0%
	CCCTACACGACTCTAATGAA+TGG	-	chr6.2:77779773-77779792	MS.gene05231:CDS	45.0%
	CTCTACCGAAAAAACGGTGT+AGG	+	chr6.2:77779696-77779715	None:intergenic	45.0%
	GAGGAATGATTGAGAATCCG+TGG	-	chr6.2:77779611-77779630	MS.gene05231:CDS	45.0%
	GAGTCTATGGGCATAGTGAA+TGG	+	chr6.2:77779214-77779233	None:intergenic	45.0%
	GAGTTATCCAACAAGGACCT+TGG	-	chr6.2:77779137-77779156	MS.gene05231:CDS	45.0%
	GATTATGTTGGACCAGAAGG+AGG	-	chr6.2:77779592-77779611	MS.gene05231:CDS	45.0%
	GGAACTGGAGAAGATACATG+AGG	-	chr6.2:77779353-77779372	MS.gene05231:CDS	45.0%
	GGAAGAGATGTGGTTACTAG+AGG	-	chr6.2:77779724-77779743	MS.gene05231:CDS	45.0%
	GGGTAGCTATCAAACAACCA+AGG	+	chr6.2:77779157-77779176	None:intergenic	45.0%
	GGTTGCATGATGGTAGGAAT+AGG	-	chr6.2:77779632-77779651	MS.gene05231:CDS	45.0%
	GGTTTGATAGGCCATACAGA+TGG	-	chr6.2:77779499-77779518	MS.gene05231:CDS	45.0%
	TGTGGAAGGACAACAGCAAT+GGG	-	chr6.2:77779552-77779571	MS.gene05231:CDS	45.0%
	TTCCTACCATCATGCAACCA+CGG	+	chr6.2:77779631-77779650	None:intergenic	45.0%
	TTGTGGAAGGACAACAGCAA+TGG	-	chr6.2:77779551-77779570	MS.gene05231:CDS	45.0%
!	CTACCCTCTAATTTGGAGCA+AGG	-	chr6.2:77779171-77779190	MS.gene05231:CDS	45.0%
!	GTCAAACGTATCAGGAGTGT+AGG	+	chr6.2:77778957-77778976	None:intergenic	45.0%
!!	GAAACGGTTTGGTTCCATCA+AGG	+	chr6.2:77779083-77779102	None:intergenic	45.0%
!!	GGAGGATGGTGCTGTTATAA+GGG	-	chr6.2:77779432-77779451	MS.gene05231:CDS	45.0%
!!	TGGAGGATGGTGCTGTTATA+AGG	-	chr6.2:77779431-77779450	MS.gene05231:CDS	45.0%
	AATGGGTGCGCTTCGATGTT+GGG	-	chr6.2:77779791-77779810	MS.gene05231:CDS	50.0%
	ATCCGTGGTTGCATGATGGT+AGG	-	chr6.2:77779626-77779645	MS.gene05231:CDS	50.0%
	GAGAATCCGTGGTTGCATGA+TGG	-	chr6.2:77779622-77779641	MS.gene05231:CDS	50.0%
	GAGACGATGGGAATTCTGCT+GGG	-	chr6.2:77779283-77779302	MS.gene05231:CDS	50.0%
	GGAATAGGGGAAGGAGAGTT+TGG	-	chr6.2:77779647-77779666	MS.gene05231:CDS	50.0%
	GGCTTCATGACTGAGACGAT+GGG	-	chr6.2:77779271-77779290	MS.gene05231:CDS	50.0%
	GTCTCAGTCATGAAGCCGAA+GGG	+	chr6.2:77779268-77779287	None:intergenic	50.0%
	GTGAAAACGGACAGGTTTGG+TGG	+	chr6.2:77779005-77779024	None:intergenic	50.0%
	GTGGAAGGACAACAGCAATG+GGG	-	chr6.2:77779553-77779572	MS.gene05231:CDS	50.0%
	TCTATGGCAGCCTAAGCATC+AGG	-	chr6.2:77779048-77779067	MS.gene05231:CDS	50.0%
	TCTCAGTCATGAAGCCGAAG+GGG	+	chr6.2:77779267-77779286	None:intergenic	50.0%
	TGAAGCCGAAGGGGAGATTA+TGG	+	chr6.2:77779258-77779277	None:intergenic	50.0%
	TGAGACGATGGGAATTCTGC+TGG	-	chr6.2:77779282-77779301	MS.gene05231:CDS	50.0%
!	GTATCAGGAGTGTAGGGTGT+TGG	+	chr6.2:77778950-77778969	None:intergenic	50.0%
!!	AACGGACAGGTTTGGTGGTT+AGG	+	chr6.2:77779000-77779019	None:intergenic	50.0%
	AGCAATGGGGACCGTATCTG+AGG	-	chr6.2:77779566-77779585	MS.gene05231:CDS	55.0%
	CGGCTTCATGACTGAGACGA+TGG	-	chr6.2:77779270-77779289	MS.gene05231:CDS	55.0%
	CGTCTCAGTCATGAAGCCGA+AGG	+	chr6.2:77779269-77779288	None:intergenic	55.0%
	GAATGGGTGCGCTTCGATGT+TGG	-	chr6.2:77779790-77779809	MS.gene05231:CDS	55.0%
!!	CAGGAGTGTAGGGTGTTGGA+TGG	+	chr6.2:77778946-77778965	None:intergenic	55.0%
!!	GAGGCTGAGAGTATCTCTGC+CGG	-	chr6.2:77779372-77779391	MS.gene05231:CDS	55.0%
	CACACCAGACGCTGCTCAAC+CGG	-	chr6.2:77779825-77779844	MS.gene05231:CDS	60.0%
!	CGCTGCTCAACCGGTTGGTT+CGG	-	chr6.2:77779834-77779853	MS.gene05231:CDS	60.0%
!	GCTGCTCAACCGGTTGGTTC+GGG	-	chr6.2:77779835-77779854	MS.gene05231:CDS	60.0%
	CCAACCGGTTGAGCAGCGTC+TGG	+	chr6.2:77779832-77779851	None:intergenic	65.0%
	CCAGACGCTGCTCAACCGGT+TGG	-	chr6.2:77779829-77779848	MS.gene05231:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	77778893	77779882	77778893	ID=MS.gene05231
chr6.2	mRNA	77778893	77779882	77778893	ID=MS.gene05231.t1;Parent=MS.gene05231
chr6.2	exon	77778893	77779882	77778893	ID=MS.gene05231.t1.exon1;Parent=MS.gene05231.t1
chr6.2	CDS	77778893	77779882	77778893	ID=cds.MS.gene05231.t1;Parent=MS.gene05231.t1

Gene Sequence

>MS.gene05231

ATGTCCCAATCCTCAAACTCTGCTCAAAATACCAATTCCACTAAAAATAGCCATCCAACACCCTACACTCCTGATACGTTTGACTACTGTCTTGTTGGCAGACTCCTAACCACCAAACCTGTCCGTTTTCACAATTTTAAAACTCGTATGTCGACTCTATGGCAGCCTAAGCATCAGGTTGACATTACCTTGATGGAACCAAACCGTTTCATGTTCCAGTTCTTTAGCTATGCTGATATGGAACGAGTTATCCAACAAGGACCTTGGTTGTTTGATAGCTACCCTCTAATTTGGAGCAAGGTTACTGATGGAAAAAATCCATTCACTATGCCCATAGACTCAATGGAATTATGGATGCAAGTCCATAATCTCCCCTTCGGCTTCATGACTGAGACGATGGGAATTCTGCTGGGTAATCATGTAGGGAAGCTAGTAAAATATGATCATGAGAACAACTATGGGAACTGGAGAAGATACATGAGGCTGAGAGTATCTCTGCCGGTTACAGAACCTTTGAAGAAGAGCTTTGAATTTGTGTTGGAGGATGGTGCTGTTATAAGGGTGAATTTCCGTTATGAGAAACCGGGAAATTTTTGTTACGAATGTGGTTTGATAGGCCATACAGATGGTTCATGCCTAAAAAGATTTGAGAAAGGTTTTGTGGAAGGACAACAGCAATGGGGACCGTATCTGAGGTCTGATTATGTTGGACCAGAAGGAGGAATGATTGAGAATCCGTGGTTGCATGATGGTAGGAATAGGGGAAGGAGAGTTTGGACTCATGCTGCTGAGTGTTCTAACCTACACCGTTTTTTCGGTAGAGTTAAAATTGGAAGAGATGTGGTTACTAGAGGACTGGTCTTTTATAGACACATTGGTGCCCTACACGACTCTAATGAATGGGTGCGCTTCGATGTTGGGAGCTTTAGTCACACACCAGACGCTGCTCAACCGGTTGGTTCGGGAACAATTCTGAATAACAGTCTGTAG

Protein sequence

>MS.gene05231.t1

MSQSSNSAQNTNSTKNSHPTPYTPDTFDYCLVGRLLTTKPVRFHNFKTRMSTLWQPKHQVDITLMEPNRFMFQFFSYADMERVIQQGPWLFDSYPLIWSKVTDGKNPFTMPIDSMELWMQVHNLPFGFMTETMGILLGNHVGKLVKYDHENNYGNWRRYMRLRVSLPVTEPLKKSFEFVLEDGAVIRVNFRYEKPGNFCYECGLIGHTDGSCLKRFEKGFVEGQQQWGPYLRSDYVGPEGGMIENPWLHDGRNRGRRVWTHAAECSNLHRFFGRVKIGRDVVTRGLVFYRHIGALHDSNEWVRFDVGSFSHTPDAAQPVGSGTILNNSL