AlfalfaGEDB | MS.gene94654

M. sativa cultivar XinJiangDaYe / MS.gene94654

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94654.t1	PNX72859.1	42	419	196	9	4	407	124	510	1.00E-75	294.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94654.t1	A0A392MN10	53.7	255	117	1	4	258	25	278	1.0e-77	300.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene28545	MS.gene94654	PPI
MS.gene94654	MS.gene08708	PPI
MS.gene008275	MS.gene94654	PPI
MS.gene94654	MS.gene86275	PPI
MS.gene94654	MS.gene034643	PPI
MS.gene94654	MS.gene60059	PPI
MS.gene94654	MS.gene038098	PPI
MS.gene94654	MS.gene071320	PPI
MS.gene94654	MS.gene012019	PPI
MS.gene94654	MS.gene46941	PPI
MS.gene94654	MS.gene012889	PPI
MS.gene94654	MS.gene038757	PPI
MS.gene94654	MS.gene000506	PPI
MS.gene94654	MS.gene015248	PPI
MS.gene94654	MS.gene030090	PPI
MS.gene94654	MS.gene39956	PPI
MS.gene94654	MS.gene028716	PPI
MS.gene94654	MS.gene74639	PPI
MS.gene94654	MS.gene071133	PPI
MS.gene94654	MS.gene60266	PPI
MS.gene94654	MS.gene98467	PPI
MS.gene94654	MS.gene013644	PPI
MS.gene94654	MS.gene024522	PPI
MS.gene94654	MS.gene54051	PPI
MS.gene94654	MS.gene22111	PPI
MS.gene94654	MS.gene049610	PPI
MS.gene94654	MS.gene010290	PPI
MS.gene94654	MS.gene93396	PPI
MS.gene94654	MS.gene37955	PPI
MS.gene94654	MS.gene95561	PPI
MS.gene94654	MS.gene05358	PPI
MS.gene94654	MS.gene92517	PPI
MS.gene94654	MS.gene34197	PPI
MS.gene96109	MS.gene94654	PPI
MS.gene94654	MS.gene045875	PPI
MS.gene94654	MS.gene010890	PPI
MS.gene94654	MS.gene055683	PPI
MS.gene94654	MS.gene061579	PPI
MS.gene94654	MS.gene05319	PPI
MS.gene94654	MS.gene69495	PPI
MS.gene94654	MS.gene032311	PPI
MS.gene94654	MS.gene41979	PPI
MS.gene94654	MS.gene04970	PPI
MS.gene94654	MS.gene26038	PPI
MS.gene94654	MS.gene060788	PPI
MS.gene94654	MS.gene98616	PPI
MS.gene94654	MS.gene43924	PPI
MS.gene94654	MS.gene23521	PPI
MS.gene94654	MS.gene41643	PPI
MS.gene94654	MS.gene77990	PPI
MS.gene94654	MS.gene062511	PPI
MS.gene94654	MS.gene008861	PPI
MS.gene94654	MS.gene065627	PPI
MS.gene94654	MS.gene027994	PPI
MS.gene016102	MS.gene94654	PPI
MS.gene46941	MS.gene94654	PPI
MS.gene94654	MS.gene96541	PPI
MS.gene96541	MS.gene94654	PPI
MS.gene94654	MS.gene26433	PPI
MS.gene94654	MS.gene007022	PPI
MS.gene94654	MS.gene045614	PPI
MS.gene94654	MS.gene064966	PPI
MS.gene94654	MS.gene69584	PPI
MS.gene94654	MS.gene036423	PPI
MS.gene94654	MS.gene52349	PPI
MS.gene94654	MS.gene76420	PPI
MS.gene94654	MS.gene30136	PPI
MS.gene94654	MS.gene46353	PPI
MS.gene94654	MS.gene65471	PPI
MS.gene94654	MS.gene060386	PPI
MS.gene93396	MS.gene94654	PPI
MS.gene94654	MS.gene61651	PPI
MS.gene94654	MS.gene29406	PPI
MS.gene94654	MS.gene63762	PPI
MS.gene94654	MS.gene09023	PPI
MS.gene94654	MS.gene88393	PPI
MS.gene94654	MS.gene054508	PPI
MS.gene94654	MS.gene95960	PPI
MS.gene94654	MS.gene053373	PPI
MS.gene94654	MS.gene041264	PPI
MS.gene94654	MS.gene35175	PPI
MS.gene56991	MS.gene94654	PPI
MS.gene94654	MS.gene63150	PPI
MS.gene94654	MS.gene035626	PPI
MS.gene94654	MS.gene44083	PPI
MS.gene94654	MS.gene000629	PPI
MS.gene20246	MS.gene94654	PPI
MS.gene94654	MS.gene072852	PPI
MS.gene94654	MS.gene65474	PPI
MS.gene94654	MS.gene018745	PPI
MS.gene94654	MS.gene025401	PPI
MS.gene94654	MS.gene34812	PPI
MS.gene94654	MS.gene57044	PPI
MS.gene94654	MS.gene015093	PPI
MS.gene94654	MS.gene04134	PPI
MS.gene94654	MS.gene06164	PPI
MS.gene94654	MS.gene50392	PPI
MS.gene94654	MS.gene22730	PPI
MS.gene94654	MS.gene000379	PPI
MS.gene94654	MS.gene032299	PPI
MS.gene94654	MS.gene017586	PPI
MS.gene94654	MS.gene037634	PPI
MS.gene94654	MS.gene057828	PPI
MS.gene94654	MS.gene08606	PPI
MS.gene94654	MS.gene048747	PPI
MS.gene94654	MS.gene000640	PPI
MS.gene94654	MS.gene061850	PPI
MS.gene94654	MS.gene09517	PPI
MS.gene94654	MS.gene98756	PPI
MS.gene94654	MS.gene87845	PPI
MS.gene94654	MS.gene57019	PPI
MS.gene94654	MS.gene85308	PPI
MS.gene94654	MS.gene64130	PPI
MS.gene94654	MS.gene62249	PPI
MS.gene94654	MS.gene58419	PPI
MS.gene69490	MS.gene94654	PPI
MS.gene94654	MS.gene71588	PPI
MS.gene015248	MS.gene94654	PPI
MS.gene94654	MS.gene046089	PPI
MS.gene94654	MS.gene053747	PPI
MS.gene94654	MS.gene28545	PPI
MS.gene013193	MS.gene94654	PPI
MS.gene94654	MS.gene021555	PPI
MS.gene08106	MS.gene94654	PPI
MS.gene94654	MS.gene36069	PPI
MS.gene94654	MS.gene71675	PPI
MS.gene94654	MS.gene044729	PPI
MS.gene94654	MS.gene008275	PPI
MS.gene94654	MS.gene60390	PPI
MS.gene94654	MS.gene039692	PPI
MS.gene94654	MS.gene55682	PPI
MS.gene94654	MS.gene002590	PPI
MS.gene94654	MS.gene81712	PPI
MS.gene94654	MS.gene008927	PPI
MS.gene94654	MS.gene005120	PPI
MS.gene94654	MS.gene65546	PPI
MS.gene94654	MS.gene025873	PPI
MS.gene94654	MS.gene05357	PPI
MS.gene94654	MS.gene024297	PPI
MS.gene94654	MS.gene059269	PPI
MS.gene94654	MS.gene35202	PPI
MS.gene94654	MS.gene22200	PPI
MS.gene94654	MS.gene057934	PPI
MS.gene94654	MS.gene37537	PPI
MS.gene94654	MS.gene024841	PPI
MS.gene94654	MS.gene069786	PPI
MS.gene94654	MS.gene42010	PPI
MS.gene94654	MS.gene025158	PPI
MS.gene94654	MS.gene40828	PPI
MS.gene038676	MS.gene94654	PPI
MS.gene94654	MS.gene87529	PPI
MS.gene94654	MS.gene51799	PPI
MS.gene94654	MS.gene98471	PPI
MS.gene94654	MS.gene062067	PPI
MS.gene94654	MS.gene97410	PPI
MS.gene94654	MS.gene56991	PPI
MS.gene94654	MS.gene013676	PPI
MS.gene94654	MS.gene013193	PPI
MS.gene94654	MS.gene40469	PPI
MS.gene94654	MS.gene65623	PPI
MS.gene94654	MS.gene021968	PPI
MS.gene94654	MS.gene012748	PPI
MS.gene94654	MS.gene84790	PPI
MS.gene94654	MS.gene063214	PPI
MS.gene94654	MS.gene66651	PPI
MS.gene94654	MS.gene025987	PPI
MS.gene94654	MS.gene043140	PPI
MS.gene94654	MS.gene02896	PPI
MS.gene94654	MS.gene72520	PPI
MS.gene94654	MS.gene61452	PPI
MS.gene94654	MS.gene05231	PPI
MS.gene94654	MS.gene03149	PPI
MS.gene94654	MS.gene042559	PPI
MS.gene94654	MS.gene46948	PPI
MS.gene94654	MS.gene29321	PPI
MS.gene94654	MS.gene037049	PPI
MS.gene94654	MS.gene24278	PPI
MS.gene42903	MS.gene94654	PPI
MS.gene94654	MS.gene34618	PPI
MS.gene94654	MS.gene59460	PPI
MS.gene94654	MS.gene060773	PPI
MS.gene94654	MS.gene66650	PPI
MS.gene94654	MS.gene009121	PPI
MS.gene94654	MS.gene08713	PPI
MS.gene94654	MS.gene09589	PPI
MS.gene94654	MS.gene70932	PPI
MS.gene042559	MS.gene94654	PPI
MS.gene22200	MS.gene94654	PPI
MS.gene94654	MS.gene024405	PPI
MS.gene94654	MS.gene067340	PPI
MS.gene94654	MS.gene021965	PPI
MS.gene94654	MS.gene018196	PPI
MS.gene94654	MS.gene32415	PPI
MS.gene94654	MS.gene24070	PPI
MS.gene94654	MS.gene83772	PPI
MS.gene94654	MS.gene059736	PPI
MS.gene94654	MS.gene85538	PPI
MS.gene94654	MS.gene26844	PPI
MS.gene94654	MS.gene64178	PPI
MS.gene94654	MS.gene96174	PPI
MS.gene34812	MS.gene94654	PPI
MS.gene94654	MS.gene074257	PPI
MS.gene94654	MS.gene04749	PPI
MS.gene94654	MS.gene073573	PPI
MS.gene063214	MS.gene94654	PPI
MS.gene94654	MS.gene69490	PPI
MS.gene94654	MS.gene44144	PPI
MS.gene94654	MS.gene05576	PPI
MS.gene94654	MS.gene060718	PPI
MS.gene035626	MS.gene94654	PPI
MS.gene94654	MS.gene96109	PPI
MS.gene94654	MS.gene63743	PPI
MS.gene94654	MS.gene016102	PPI
MS.gene067340	MS.gene94654	PPI
MS.gene94654	MS.gene48477	PPI
MS.gene94654	MS.gene055223	PPI
MS.gene94654	MS.gene068629	PPI
MS.gene94654	MS.gene08106	PPI
MS.gene94654	MS.gene027169	PPI
MS.gene94654	MS.gene004417	PPI
MS.gene94654	MS.gene019177	PPI
MS.gene94654	MS.gene031967	PPI
MS.gene94654	MS.gene05436	PPI
MS.gene69495	MS.gene94654	PPI
MS.gene94654	MS.gene038623	PPI
MS.gene05357	MS.gene94654	PPI
MS.gene94654	MS.gene055560	PPI
MS.gene94654	MS.gene009213	PPI
MS.gene94654	MS.gene98127	PPI
MS.gene94654	MS.gene003264	PPI
MS.gene94654	MS.gene054652	PPI
MS.gene94654	MS.gene000634	PPI
MS.gene94654	MS.gene20246	PPI
MS.gene94654	MS.gene86849	PPI
MS.gene94654	MS.gene049512	PPI
MS.gene94654	MS.gene27329	PPI
MS.gene021968	MS.gene94654	PPI
MS.gene94654	MS.gene41393	PPI
MS.gene94654	MS.gene42903	PPI
MS.gene94654	MS.gene30208	PPI
MS.gene94654	MS.gene80362	PPI
MS.gene94654	MS.gene89815	PPI
MS.gene94654	MS.gene010656	PPI
MS.gene94654	MS.gene016632	PPI
MS.gene94654	MS.gene018474	PPI
MS.gene94654	MS.gene048088	PPI
MS.gene94654	MS.gene86868	PPI
MS.gene94654	MS.gene058889	PPI
MS.gene94654	MS.gene96163	PPI
MS.gene94654	MS.gene050679	PPI
MS.gene94654	MS.gene34463	PPI
MS.gene94654	MS.gene042614	PPI
MS.gene94654	MS.gene026680	PPI
MS.gene94654	MS.gene61499	PPI
MS.gene94654	MS.gene51804	PPI
MS.gene94654	MS.gene051449	PPI
MS.gene94654	MS.gene042651	PPI
MS.gene94654	MS.gene019165	PPI
MS.gene94654	MS.gene81194	PPI
MS.gene94654	MS.gene038394	PPI
MS.gene94654	MS.gene019001	PPI
MS.gene94654	MS.gene038676	PPI
MS.gene94654	MS.gene53904	PPI
MS.gene94654	MS.gene73261	PPI
MS.gene94654	MS.gene025675	PPI
MS.gene94654	MS.gene055546	PPI
MS.gene94654	MS.gene71637	PPI
MS.gene94654	MS.gene55209	PPI
MS.gene94654	MS.gene28718	PPI
MS.gene94654	MS.gene022929	PPI
MS.gene94654	MS.gene85295	PPI
MS.gene72520	MS.gene94654	PPI
MS.gene94654	MS.gene009781	PPI
MS.gene30208	MS.gene94654	PPI
MS.gene94654	MS.gene021647	PPI
MS.gene94654	MS.gene047954	PPI
MS.gene025873	MS.gene94654	PPI
MS.gene94654	MS.gene071784	PPI
MS.gene94654	MS.gene048342	PPI
MS.gene94654	MS.gene069624	PPI
MS.gene05358	MS.gene94654	PPI
MS.gene94654	MS.gene83979	PPI
MS.gene94654	MS.gene21066	PPI
MS.gene95561	MS.gene94654	PPI
MS.gene94654	MS.gene013890	PPI
MS.gene94654	MS.gene073582	PPI
MS.gene065627	MS.gene94654	PPI
MS.gene94654	MS.gene016719	PPI
MS.gene57019	MS.gene94654	PPI
MS.gene94654	MS.gene048072	PPI
MS.gene94654	MS.gene028714	PPI
MS.gene94654	MS.gene042027	PPI
MS.gene94654	MS.gene048706	PPI
MS.gene94654	MS.gene69586	PPI
MS.gene94654	MS.gene45083	PPI
MS.gene94654	MS.gene049561	PPI
MS.gene94654	MS.gene008658	PPI
MS.gene94654	MS.gene47792	PPI
MS.gene94654	MS.gene49057	PPI
MS.gene94654	MS.gene03936	PPI
MS.gene94654	MS.gene43393	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94654.t1	MTR_3g053920	41.888	339	122	6	4	338	5	272	1.08e-72	231
MS.gene94654.t1	MTR_2g061500	54.839	124	56	0	5	128	6	129	1.00e-43	151
MS.gene94654.t1	MTR_6g033215	32.022	178	119	1	23	198	117	294	1.90e-32	125
MS.gene94654.t1	MTR_4g103530	55.208	96	41	1	58	153	5	98	1.99e-30	117
MS.gene94654.t1	MTR_0010s0370	35.429	175	105	5	7	175	4	176	3.83e-30	116
MS.gene94654.t1	MTR_0010s0350	35.429	175	105	5	7	175	4	176	3.83e-30	116
MS.gene94654.t1	MTR_7g011710	56.471	85	37	0	54	138	1	85	8.52e-29	111
MS.gene94654.t1	MTR_1g036720	35.354	99	62	1	6	102	3	101	2.63e-15	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94654.t1	AT5G36228	26.943	193	137	2	35	225	39	229	6.86e-16	79.3
MS.gene94654.t1	AT2G17920	27.815	151	99	3	70	215	74	219	3.05e-14	73.9
MS.gene94654.t1	AT2G41590	28.571	154	100	4	66	215	72	219	4.39e-14	73.9
MS.gene94654.t1	AT3G57587	29.032	155	98	4	66	215	72	219	5.07e-14	73.9
MS.gene94654.t1	AT2G13450	28.947	152	93	4	71	214	74	218	2.02e-13	72.4
MS.gene94654.t1	AT4G02000	27.660	141	88	3	82	215	6	139	2.01e-11	65.5
MS.gene94654.t1	AT2G01050	26.263	198	120	10	30	216	76	258	6.06e-11	65.1

Find 136 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATACACATCTGAACCGATTT+TGG	0.193331	5.1:-74864175	MS.gene94654:CDS
ATCTGAATTTCCTTGACATT+TGG	0.223105	5.1:-74865966	MS.gene94654:CDS
TCTGAATTTCCTTGACATTT+GGG	0.252919	5.1:-74865965	MS.gene94654:CDS
CCTTAGCAAGTAGTTGCATT+TGG	0.258387	5.1:+74865389	None:intergenic
GTTTGAAAATGAGTGGAAAT+TGG	0.269651	5.1:-74864238	MS.gene94654:CDS
TCCCCTCCCACATACATAAT+TGG	0.273701	5.1:+74865079	None:intergenic
AGTAGATTGTTGCACATTCT+TGG	0.280868	5.1:+74865171	None:intergenic
CATCAAGTCGGTGGCGAAAC+TGG	0.288198	5.1:+74866082	None:intergenic
TTTCCTTGACATTTGGGTTC+AGG	0.291183	5.1:-74865959	MS.gene94654:CDS
AGATCAAAACTGCTTATTTC+CGG	0.314616	5.1:-74866179	MS.gene94654:CDS
GAAATTCATGCGATTAAGTC+TGG	0.315272	5.1:-74865424	MS.gene94654:CDS
TCCTCTAGTGCTTGGTTTAG+AGG	0.323013	5.1:-74865284	MS.gene94654:CDS
AGGATGACGGTCACCGCGGC+TGG	0.327235	5.1:-74865645	MS.gene94654:CDS
CTTAGCAAGTAGTTGCATTT+GGG	0.332965	5.1:+74865390	None:intergenic
GGCCTTTGTCTTGTAGGTAT+AGG	0.343305	5.1:+74865556	None:intergenic
CAGGTCCATAACTTGCCATT+TGG	0.345980	5.1:-74865940	MS.gene94654:CDS
AATTCTGATGAGACGGGCTC+TGG	0.353000	5.1:-74865220	MS.gene94654:CDS
GGAAAAGGCCTTTGTCTTGT+AGG	0.353766	5.1:+74865550	None:intergenic
GCCTTTGTCTTGTAGGTATA+GGG	0.359035	5.1:+74865557	None:intergenic
CGGTGAAGACGTTCCAAAAT+CGG	0.371625	5.1:+74864162	None:intergenic
GATCAAAACTGCTTATTTCC+GGG	0.378402	5.1:-74866178	MS.gene94654:CDS
TAGAATATCTGCTGTTCAAC+AGG	0.390727	5.1:-74865446	MS.gene94654:CDS
TGATGAGACGGGCTCTGGGC+TGG	0.410689	5.1:-74865215	MS.gene94654:CDS
TGCTTATTTCCGGGAGCGCA+TGG	0.410942	5.1:-74866169	MS.gene94654:CDS
TCAGGCAGTGTTCCATGATC+TGG	0.411624	5.1:+74866226	None:intergenic
GGGAGCGCATGGGGTACATT+TGG	0.412599	5.1:-74866158	MS.gene94654:CDS
GTCACCGCGGCTGGGGTGAA+TGG	0.420073	5.1:-74865636	MS.gene94654:CDS
CATCAGCGGCTACAGGATCT+AGG	0.420308	5.1:+74866250	None:intergenic
AAATGAGTGGAAATTGGAAC+CGG	0.430637	5.1:-74864232	MS.gene94654:CDS
CCTGCATTTCCTTGCGAAGA+TGG	0.433205	5.1:+74865256	None:intergenic
TTTCCGAGACCGTCTACAAT+TGG	0.440040	5.1:+74864107	None:intergenic
TGTTACATGTGTGGTATACT+TGG	0.447449	5.1:-74865709	MS.gene94654:CDS
ATCATGGAACACTGCCTGAT+TGG	0.448132	5.1:-74866222	MS.gene94654:CDS
TACCGTGACCGGTACGAGGC+TGG	0.451502	5.1:+74865911	None:intergenic
TCCAATTATGTATGTGGGAG+GGG	0.455209	5.1:-74865080	MS.gene94654:CDS
ATCTGGACATCAGCGGCTAC+AGG	0.457125	5.1:+74866243	None:intergenic
TTTCCTGCTAATGTCAGTCC+AGG	0.459027	5.1:-74865532	MS.gene94654:CDS
TGCTCCATCTCAAACAATCT+AGG	0.464557	5.1:+74865667	None:intergenic
GGGTGAATGGCTTCGTCCTA+CGG	0.468614	5.1:-74865623	MS.gene94654:CDS
CCAACATAAGTGTTCATTTG+CGG	0.472954	5.1:+74865469	None:intergenic
ACCCCTGGACTGACATTAGC+AGG	0.474214	5.1:+74865529	None:intergenic
GGGTTGACCTTGTCTGATGA+AGG	0.476432	5.1:-74866285	MS.gene94654:CDS
AAATGTACCCCATGCGCTCC+CGG	0.477475	5.1:+74866160	None:intergenic
CCTCGTACCGGTCACGGTAT+TGG	0.483532	5.1:-74865907	MS.gene94654:CDS
TCTAGTGTAGGAAGGGTTGC+GGG	0.484858	5.1:-74865118	MS.gene94654:CDS
GGATGACGGTCACCGCGGCT+GGG	0.484866	5.1:-74865644	MS.gene94654:CDS
GATGACGGTCACCGCGGCTG+GGG	0.486900	5.1:-74865643	MS.gene94654:CDS
GCTTATTTCCGGGAGCGCAT+GGG	0.487197	5.1:-74866168	MS.gene94654:CDS
TGTAGGTCCAATTATGTATG+TGG	0.490686	5.1:-74865086	MS.gene94654:CDS
GTGGAGAGGATATGTTTCAT+TGG	0.490834	5.1:-74864137	MS.gene94654:CDS
ATGCCAATTGTAGACGGTCT+CGG	0.497662	5.1:-74864110	MS.gene94654:CDS
CAGATAACAAACGACCAATC+AGG	0.498270	5.1:+74866208	None:intergenic
TTCCTGCTAATGTCAGTCCA+GGG	0.500076	5.1:-74865531	MS.gene94654:CDS
GGACTGACATTAGCAGGAAA+AGG	0.501332	5.1:+74865535	None:intergenic
CAACACCGTCCTCTAGTGCT+TGG	0.503725	5.1:-74865292	MS.gene94654:CDS
TTGACAACGTCGTTGAAACC+CGG	0.507632	5.1:+74864213	None:intergenic
GGATGTTGAGATGTCTGTAA+TGG	0.509606	5.1:-74865059	MS.gene94654:CDS
TTAGACCAAATGGCAAGTTA+TGG	0.510771	5.1:+74865935	None:intergenic
GTCTAGTGTAGGAAGGGTTG+CGG	0.512720	5.1:-74865119	MS.gene94654:CDS
GAAACCGATCGTCATCAGCC+TGG	0.522943	5.1:+74866109	None:intergenic
ATTCTGATGAGACGGGCTCT+GGG	0.524718	5.1:-74865219	MS.gene94654:CDS
CCATCTTCGCAAGGAAATGC+AGG	0.528703	5.1:-74865256	MS.gene94654:CDS
ATCCAGCCTCGTACCGGTCA+CGG	0.528988	5.1:-74865913	MS.gene94654:CDS
ACTACAACCTTGTGGTGGTA+CGG	0.530016	5.1:-74866020	MS.gene94654:CDS
TGGTACTGTTACTGCGACTG+TGG	0.530171	5.1:-74865989	MS.gene94654:CDS
ACGGTCTCGGAAAGATTTAG+AGG	0.531367	5.1:-74864097	None:intergenic
AGCCGCTGATGTCCAGATCA+TGG	0.533568	5.1:-74866238	MS.gene94654:CDS
CCAAATGCAACTACTTGCTA+AGG	0.534157	5.1:-74865389	MS.gene94654:CDS
ACGACGTTGTCAAAGAAAGT+TGG	0.534679	5.1:-74864203	MS.gene94654:CDS
GATTAAGTCTGGGTTATTGA+CGG	0.535240	5.1:-74865413	MS.gene94654:CDS
CAGCAGCTGTGTGGAAGATG+AGG	0.535722	5.1:+74865327	None:intergenic
TAGATTGTTTGAGATGGAGC+AGG	0.536001	5.1:-74865665	MS.gene94654:CDS
GCCGCTGATTTGAACCTCGA+AGG	0.537830	5.1:-74866306	MS.gene94654:CDS
CGAGGCTGGATTAGACCAAA+TGG	0.541627	5.1:+74865925	None:intergenic
AATGACAATTCTGATGAGAC+GGG	0.543597	5.1:-74865226	MS.gene94654:CDS
CTTATTTCCGGGAGCGCATG+GGG	0.544459	5.1:-74866167	MS.gene94654:CDS
CCGCTGATTTGAACCTCGAA+GGG	0.547556	5.1:-74866305	MS.gene94654:CDS
AAAATGACCAATACCGTGAC+CGG	0.547620	5.1:+74865900	None:intergenic
GCGGGTAAGAACAACGCTGC+TGG	0.547910	5.1:-74865367	MS.gene94654:CDS
GAATGACAATTCTGATGAGA+CGG	0.548602	5.1:-74865227	MS.gene94654:CDS
GCTGCTAAAGTTCTCGATGA+AGG	0.549715	5.1:-74866060	MS.gene94654:CDS
ATGTCCTAGATTGTTTGAGA+TGG	0.550256	5.1:-74865671	MS.gene94654:CDS
ACTTCAAGTTTGAAAATGAG+TGG	0.550663	5.1:-74864245	MS.gene94654:intron
CAAGAAAGTCTCTATCATCC+AGG	0.551748	5.1:-74866127	MS.gene94654:CDS
CGTCCTACGGTTCGCCGTGT+CGG	0.553198	5.1:-74865610	MS.gene94654:CDS
GGTCTAATCCAGCCTCGTAC+CGG	0.554997	5.1:-74865919	MS.gene94654:CDS
GTGGTGGTACGGATCGCACC+TGG	0.556256	5.1:-74866009	MS.gene94654:CDS
CATGAAGATGTGTCTAGTGT+AGG	0.565586	5.1:-74865130	MS.gene94654:CDS
TTGGAACGTCTTCACCGTTG+TGG	0.568868	5.1:-74864156	MS.gene94654:CDS
AATGTGCAACAATCTACTGC+AGG	0.569350	5.1:-74865166	MS.gene94654:CDS
AAATTCATGCGATTAAGTCT+GGG	0.570439	5.1:-74865423	MS.gene94654:CDS
CCGCAAATGAACACTTATGT+TGG	0.572874	5.1:-74865469	MS.gene94654:CDS
ATGATAGAGACTTTCTTGAC+TGG	0.576891	5.1:+74866132	None:intergenic
GCCTCTAAACCAAGCACTAG+AGG	0.577762	5.1:+74865283	None:intergenic
ACATGCTTCAGTTTCAACTT+CGG	0.577774	5.1:-74865497	MS.gene94654:CDS
TCCTGCTAATGTCAGTCCAG+GGG	0.577850	5.1:-74865530	MS.gene94654:CDS
AAGATGTGTCTAGTGTAGGA+AGG	0.578088	5.1:-74865126	MS.gene94654:CDS
AGCAGCTGTGTGGAAGATGA+GGG	0.578421	5.1:+74865328	None:intergenic
GTCCAATTATGTATGTGGGA+GGG	0.581552	5.1:-74865081	MS.gene94654:CDS
TTTGAGATGGAGCAGGATGA+CGG	0.582685	5.1:-74865658	MS.gene94654:CDS
GCGTACTTTCAGACGCATGT+AGG	0.593140	5.1:+74865831	None:intergenic
TCATCCAGGCTGATGACGAT+CGG	0.594987	5.1:-74866113	MS.gene94654:CDS
GAGGCAGCGCCATCTTCGCA+AGG	0.595065	5.1:-74865265	MS.gene94654:CDS
CAGACAAGGTCAACCCTTCG+AGG	0.598822	5.1:+74866292	None:intergenic
AGATGTGTCTAGTGTAGGAA+GGG	0.598978	5.1:-74865125	MS.gene94654:CDS
ATGCAACTACTTGCTAAGGC+GGG	0.599446	5.1:-74865385	MS.gene94654:CDS
GGACCTGAACCCAAATGTCA+AGG	0.606018	5.1:+74865956	None:intergenic
CCCTTCGAGGTTCAAATCAG+CGG	0.611324	5.1:+74866305	None:intergenic
GTACCGACACGGCGAACCGT+AGG	0.612906	5.1:+74865607	None:intergenic
GCATGTTGTTGTGACACCCC+TGG	0.615395	5.1:+74865514	None:intergenic
GGAGTCATGCCAATTGTAGA+CGG	0.620927	5.1:-74864116	MS.gene94654:CDS
GTTGCGGGTAGCAACAATGT+AGG	0.621012	5.1:-74865103	MS.gene94654:CDS
TTGCAAGTGCGGGTGCGTGA+CGG	0.622153	5.1:-74865778	MS.gene94654:CDS
GTAGGTCCAATTATGTATGT+GGG	0.622970	5.1:-74865085	MS.gene94654:CDS
ACAGTCGCAGTAACAGTACC+AGG	0.631733	5.1:+74865991	None:intergenic
GGTCCAATTATGTATGTGGG+AGG	0.633777	5.1:-74865082	MS.gene94654:CDS
GAAGCCATTCACCCCAGCCG+CGG	0.641809	5.1:+74865632	None:intergenic
ACGTCTTCACCGTTGTGGAG+AGG	0.641832	5.1:-74864151	MS.gene94654:CDS
CCAATACCGTGACCGGTACG+AGG	0.644327	5.1:+74865907	None:intergenic
CTTTAGCAGCATCAAGTCGG+TGG	0.644721	5.1:+74866073	None:intergenic
AATGCAACTACTTGCTAAGG+CGG	0.648008	5.1:-74865386	MS.gene94654:CDS
AATGAGTGGAAATTGGAACC+GGG	0.648966	5.1:-74864231	MS.gene94654:CDS
TTCCATGATCTGGACATCAG+CGG	0.652956	5.1:+74866236	None:intergenic
GAACTTTAGCAGCATCAAGT+CGG	0.654580	5.1:+74866070	None:intergenic
ACCCTATACCTACAAGACAA+AGG	0.657093	5.1:-74865558	MS.gene94654:CDS
CTAAACCAAGCACTAGAGGA+CGG	0.659036	5.1:+74865287	None:intergenic
AGTTAAACCTTCATCAGACA+AGG	0.661343	5.1:+74866278	None:intergenic
GTGCGATCCGTACCACCACA+AGG	0.669962	5.1:+74866013	None:intergenic
TTGTCGCAGCTGTACCGACA+CGG	0.671222	5.1:+74865596	None:intergenic
AATCGGTTCAGATGTGTATG+CGG	0.681134	5.1:+74864179	None:intergenic
AAGTTCTCGATGAAGGTCCG+TGG	0.684631	5.1:-74866053	MS.gene94654:CDS
GAAACATATCCTCTCCACAA+CGG	0.712007	5.1:+74864142	None:intergenic
TTGTAGTTGTCATACAACCA+CGG	0.725301	5.1:+74866036	None:intergenic
GTATGACAACTACAACCTTG+TGG	0.726162	5.1:-74866028	MS.gene94654:CDS
GAGCAGGATGACGGTCACCG+CGG	0.741458	5.1:-74865649	MS.gene94654:CDS
TGACAACTACAACCTTGTGG+TGG	0.764598	5.1:-74866025	MS.gene94654:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAATTAATGATTGTGAAAA+AGG	-	chr5.1:74865707-74865726	MS.gene94654:CDS	20.0%
!!!	TATTTTAAGTATGAAAAGCT+TGG	-	chr5.1:74864670-74864689	MS.gene94654:intron	20.0%
!!	GAAGTTCCTCTATTTTATTA+CGG	+	chr5.1:74865559-74865578	None:intergenic	25.0%
!!!	ACTTGCAATTCATTTTTCAA+AGG	+	chr5.1:74864619-74864638	None:intergenic	25.0%
	AAATTCATGCGATTAAGTCT+GGG	-	chr5.1:74864986-74865005	MS.gene94654:intron	30.0%
	AGATCAAAACTGCTTATTTC+CGG	-	chr5.1:74864230-74864249	MS.gene94654:CDS	30.0%
	ATCTGAATTTCCTTGACATT+TGG	-	chr5.1:74864443-74864462	MS.gene94654:intron	30.0%
	GTTTGAAAATGAGTGGAAAT+TGG	-	chr5.1:74866171-74866190	MS.gene94654:CDS	30.0%
	TCTGAATTTCCTTGACATTT+GGG	-	chr5.1:74864444-74864463	MS.gene94654:intron	30.0%
	TGAAAAATGAATTGCAAGTG+CGG	-	chr5.1:74864620-74864639	MS.gene94654:intron	30.0%
!	ACTTCAAGTTTGAAAATGAG+TGG	-	chr5.1:74866164-74866183	MS.gene94654:CDS	30.0%
!	CATTTTCAAACTTGAAGTTG+CGG	+	chr5.1:74866162-74866181	None:intergenic	30.0%
!!	TCTTTTTCTGTTGATTGTGT+TGG	-	chr5.1:74865602-74865621	MS.gene94654:CDS	30.0%
	AAATGAGTGGAAATTGGAAC+CGG	-	chr5.1:74866177-74866196	MS.gene94654:CDS	35.0%
	AAGTTCACAAGGGAAGAATT+AGG	+	chr5.1:74866067-74866086	None:intergenic	35.0%
	AATGACAATTCTGATGAGAC+GGG	-	chr5.1:74865183-74865202	MS.gene94654:CDS	35.0%
	ACATGCTTCAGTTTCAACTT+CGG	-	chr5.1:74864912-74864931	MS.gene94654:intron	35.0%
	ACATTAGGATCAGCATACAA+AGG	+	chr5.1:74865381-74865400	None:intergenic	35.0%
	AGTAGATTGTTGCACATTCT+TGG	+	chr5.1:74865241-74865260	None:intergenic	35.0%
	AGTCTGCCGTAATAAAATAG+AGG	-	chr5.1:74865550-74865569	MS.gene94654:CDS	35.0%
	AGTTAAACCTTCATCAGACA+AGG	+	chr5.1:74864134-74864153	None:intergenic	35.0%
	ATGATAGAGACTTTCTTGAC+TGG	+	chr5.1:74864280-74864299	None:intergenic	35.0%
	ATGGATGCAAGTGAAAAAAG+GGG	-	chr5.1:74865860-74865879	MS.gene94654:CDS	35.0%
	ATGTCCTAGATTGTTTGAGA+TGG	-	chr5.1:74864738-74864757	MS.gene94654:intron	35.0%
	CATGGATGCAAGTGAAAAAA+GGG	-	chr5.1:74865859-74865878	MS.gene94654:CDS	35.0%
	CCAACATAAGTGTTCATTTG+CGG	+	chr5.1:74864943-74864962	None:intergenic	35.0%
	CTAATTAGACTCATTCTCCT+TGG	+	chr5.1:74865431-74865450	None:intergenic	35.0%
	CTTAGCAAGTAGTTGCATTT+GGG	+	chr5.1:74865022-74865041	None:intergenic	35.0%
	GAAAAATGAATTGCAAGTGC+GGG	-	chr5.1:74864621-74864640	MS.gene94654:intron	35.0%
	GAAATTCATGCGATTAAGTC+TGG	-	chr5.1:74864985-74865004	MS.gene94654:intron	35.0%
	GAATGACAATTCTGATGAGA+CGG	-	chr5.1:74865182-74865201	MS.gene94654:CDS	35.0%
	GATCAAAACTGCTTATTTCC+GGG	-	chr5.1:74864231-74864250	MS.gene94654:CDS	35.0%
	GATTAAGTCTGGGTTATTGA+CGG	-	chr5.1:74864996-74865015	MS.gene94654:intron	35.0%
	GGAGAATGAGTCTAATTAGA+TGG	-	chr5.1:74865432-74865451	MS.gene94654:CDS	35.0%
	GTAGGTCCAATTATGTATGT+GGG	-	chr5.1:74865324-74865343	MS.gene94654:CDS	35.0%
	GTATGCTGATCCTAATGTAA+AGG	-	chr5.1:74865383-74865402	MS.gene94654:CDS	35.0%
	GTCAATTATCCCATCAATCT+AGG	-	chr5.1:74865801-74865820	MS.gene94654:CDS	35.0%
	GTCTAATTAGATGGAATTGC+CGG	-	chr5.1:74865441-74865460	MS.gene94654:CDS	35.0%
	TAATTCTTCCCTTGTGAACT+TGG	-	chr5.1:74866066-74866085	MS.gene94654:CDS	35.0%
	TAGAATATCTGCTGTTCAAC+AGG	-	chr5.1:74864963-74864982	MS.gene94654:intron	35.0%
	TCATGGATGCAAGTGAAAAA+AGG	-	chr5.1:74865858-74865877	MS.gene94654:CDS	35.0%
	TCTAATTAGATGGAATTGCC+GGG	-	chr5.1:74865442-74865461	MS.gene94654:CDS	35.0%
	TGTAGGTCCAATTATGTATG+TGG	-	chr5.1:74865323-74865342	MS.gene94654:CDS	35.0%
	TGTTACATGTGTGGTATACT+TGG	-	chr5.1:74864700-74864719	MS.gene94654:intron	35.0%
	TTAGACCAAATGGCAAGTTA+TGG	+	chr5.1:74864477-74864496	None:intergenic	35.0%
	TTCAAATGAGGAATTGCACT+CGG	+	chr5.1:74865479-74865498	None:intergenic	35.0%
	TTGTAGTTGTCATACAACCA+CGG	+	chr5.1:74864376-74864395	None:intergenic	35.0%
	TTTACTTGGTTCAAAAGCCT+TGG	-	chr5.1:74865974-74865993	MS.gene94654:CDS	35.0%
!	AGGCTTTTGAACCAAGTAAA+CGG	+	chr5.1:74865974-74865993	None:intergenic	35.0%
!	GGTGATTTTTGTTACATGTG+TGG	-	chr5.1:74864691-74864710	MS.gene94654:intron	35.0%
!	GGTTTTTATGGTTACCCAAA+CGG	-	chr5.1:74865746-74865765	MS.gene94654:CDS	35.0%
!	GTTTTTATGGTTACCCAAAC+GGG	-	chr5.1:74865747-74865766	MS.gene94654:CDS	35.0%
!	TTTTTATGGTTACCCAAACG+GGG	-	chr5.1:74865748-74865767	MS.gene94654:CDS	35.0%
!!	ATACACATCTGAACCGATTT+TGG	-	chr5.1:74866234-74866253	MS.gene94654:CDS	35.0%
!!	GAGGAACTTCGTTATTTTCT+CGG	-	chr5.1:74865569-74865588	MS.gene94654:CDS	35.0%
!!	TTCTGGTTTATCTGATGTTC+CGG	-	chr5.1:74865937-74865956	MS.gene94654:CDS	35.0%
	AAAAACCTGTTAACCGCCAT+GGG	+	chr5.1:74865733-74865752	None:intergenic	40.0%
	AAAATGACCAATACCGTGAC+CGG	+	chr5.1:74864512-74864531	None:intergenic	40.0%
	AAATCACCGAAAATGCACCA+TGG	+	chr5.1:74865830-74865849	None:intergenic	40.0%
	AAGATGTGTCTAGTGTAGGA+AGG	-	chr5.1:74865283-74865302	MS.gene94654:CDS	40.0%
	AATGAGTGGAAATTGGAACC+GGG	-	chr5.1:74866178-74866197	MS.gene94654:CDS	40.0%
	AATGTGCAACAATCTACTGC+AGG	-	chr5.1:74865243-74865262	MS.gene94654:CDS	40.0%
	ACCATGGAATCCTAGATTGA+TGG	+	chr5.1:74865814-74865833	None:intergenic	40.0%
	ACCCTATACCTACAAGACAA+AGG	-	chr5.1:74864851-74864870	MS.gene94654:intron	40.0%
	ACGACGTTGTCAAAGAAAGT+TGG	-	chr5.1:74866206-74866225	MS.gene94654:CDS	40.0%
	AGATGTGTCTAGTGTAGGAA+GGG	-	chr5.1:74865284-74865303	MS.gene94654:CDS	40.0%
	AGGAAAGCTGTGTTAGATTC+TGG	-	chr5.1:74865920-74865939	MS.gene94654:CDS	40.0%
	ATGATTGTGAAAAAGGCCCA+TGG	-	chr5.1:74865714-74865733	MS.gene94654:CDS	40.0%
	CAAGAAAGTCTCTATCATCC+AGG	-	chr5.1:74864282-74864301	MS.gene94654:intron	40.0%
	CAGATAACAAACGACCAATC+AGG	+	chr5.1:74864204-74864223	None:intergenic	40.0%
	CATGAAGATGTGTCTAGTGT+AGG	-	chr5.1:74865279-74865298	MS.gene94654:CDS	40.0%
	CCATGGAATCCTAGATTGAT+GGG	+	chr5.1:74865813-74865832	None:intergenic	40.0%
	CCGCAAATGAACACTTATGT+TGG	-	chr5.1:74864940-74864959	MS.gene94654:intron	40.0%
	CCTAACCCAGTATTATAAGC+CGG	-	chr5.1:74865502-74865521	MS.gene94654:CDS	40.0%
	CCTTAGCAAGTAGTTGCATT+TGG	+	chr5.1:74865023-74865042	None:intergenic	40.0%
	CTAATTAGATGGAATTGCCG+GGG	-	chr5.1:74865443-74865462	MS.gene94654:CDS	40.0%
	CTAGTGTCTCGCTAAGAAAA+AGG	+	chr5.1:74865532-74865551	None:intergenic	40.0%
	GAAACATATCCTCTCCACAA+CGG	+	chr5.1:74866270-74866289	None:intergenic	40.0%
	GAACTTTAGCAGCATCAAGT+CGG	+	chr5.1:74864342-74864361	None:intergenic	40.0%
	GCCTTTGTCTTGTAGGTATA+GGG	+	chr5.1:74864855-74864874	None:intergenic	40.0%
	GGATGTTGAGATGTCTGTAA+TGG	-	chr5.1:74865350-74865369	MS.gene94654:CDS	40.0%
	GTATGACAACTACAACCTTG+TGG	-	chr5.1:74864381-74864400	MS.gene94654:intron	40.0%
	GTCCAATTATGTATGTGGGA+GGG	-	chr5.1:74865328-74865347	MS.gene94654:CDS	40.0%
	GTGGAGAGGATATGTTTCAT+TGG	-	chr5.1:74866272-74866291	MS.gene94654:CDS	40.0%
	TAAAAACCTGTTAACCGCCA+TGG	+	chr5.1:74865734-74865753	None:intergenic	40.0%
	TCAAATGAGGAATTGCACTC+GGG	+	chr5.1:74865478-74865497	None:intergenic	40.0%
	TCAGAACCTGCCTTTACATT+AGG	+	chr5.1:74865396-74865415	None:intergenic	40.0%
	TCCAATTATGTATGTGGGAG+GGG	-	chr5.1:74865329-74865348	MS.gene94654:CDS	40.0%
	TCGCTAAGAAAAAGGATAGC+CGG	+	chr5.1:74865524-74865543	None:intergenic	40.0%
	TGCTCCATCTCAAACAATCT+AGG	+	chr5.1:74864745-74864764	None:intergenic	40.0%
!	AATCGGTTCAGATGTGTATG+CGG	+	chr5.1:74866233-74866252	None:intergenic	40.0%
!	AATGCAACTACTTGCTAAGG+CGG	-	chr5.1:74865023-74865042	MS.gene94654:intron	40.0%
!	AGGATTCCATGGTGCATTTT+CGG	-	chr5.1:74865821-74865840	MS.gene94654:CDS	40.0%
!	ATTGGCTTATCAATGGCTTC+AGG	-	chr5.1:74865900-74865919	MS.gene94654:CDS	40.0%
!	CCAAATGCAACTACTTGCTA+AGG	-	chr5.1:74865020-74865039	MS.gene94654:intron	40.0%
!	GATAGCCGGCTTATAATACT+GGG	+	chr5.1:74865510-74865529	None:intergenic	40.0%
!	GTTGATTGTGTTGGTAGAAG+TGG	-	chr5.1:74865611-74865630	MS.gene94654:CDS	40.0%
!	TAGATTGTTTGAGATGGAGC+AGG	-	chr5.1:74864744-74864763	MS.gene94654:intron	40.0%
!	TGGCGGTTAACAGGTTTTTA+TGG	-	chr5.1:74865734-74865753	MS.gene94654:CDS	40.0%
!	TGGGTTAGGTACTTCAAATG+AGG	+	chr5.1:74865491-74865510	None:intergenic	40.0%
!	TTTCCAGGAGCTCTAACAAT+TGG	+	chr5.1:74866136-74866155	None:intergenic	40.0%
!	TTTCCTTGACATTTGGGTTC+AGG	-	chr5.1:74864450-74864469	MS.gene94654:intron	40.0%
!	TTTTATGGTTACCCAAACGG+GGG	-	chr5.1:74865749-74865768	MS.gene94654:CDS	40.0%
!!	CGGTGATTTTAACGACATCA+TGG	-	chr5.1:74865841-74865860	MS.gene94654:CDS	40.0%
!!!	CACGGTATTGGTCATTTTCA+CGG	-	chr5.1:74864514-74864533	MS.gene94654:intron	40.0%
!!!	GTGGCGGTTTAGCTTTATTT+TGG	-	chr5.1:74865630-74865649	MS.gene94654:CDS	40.0%
	AACCAGTAGTTGCTAGAGCT+CGG	+	chr5.1:74866030-74866049	None:intergenic	45.0%
	ACTACAACCTTGTGGTGGTA+CGG	-	chr5.1:74864389-74864408	MS.gene94654:intron	45.0%
	ACTCCAATTGTTAGAGCTCC+TGG	-	chr5.1:74866130-74866149	MS.gene94654:CDS	45.0%
	ATCATGGAACACTGCCTGAT+TGG	-	chr5.1:74864187-74864206	MS.gene94654:CDS	45.0%
	ATGCCAATTGTAGACGGTCT+CGG	-	chr5.1:74866299-74866318	MS.gene94654:CDS	45.0%
	ATTGTGAAAAAGGCCCATGG+CGG	-	chr5.1:74865717-74865736	MS.gene94654:CDS	45.0%
	CAAGATGGGATAGTGATCAG+AGG	+	chr5.1:74866101-74866120	None:intergenic	45.0%
	CCGGCTTATAATACTGGGTT+AGG	+	chr5.1:74865505-74865524	None:intergenic	45.0%
	CGGTGAAGACGTTCCAAAAT+CGG	+	chr5.1:74866250-74866269	None:intergenic	45.0%
	CTAAACCAAGCACTAGAGGA+CGG	+	chr5.1:74865125-74865144	None:intergenic	45.0%
	GACGAAATCTTCGCTCGAAT+TGG	-	chr5.1:74865882-74865901	MS.gene94654:CDS	45.0%
	GCTGATCCTAATGTAAAGGC+AGG	-	chr5.1:74865387-74865406	MS.gene94654:CDS	45.0%
	GCTGCTAAAGTTCTCGATGA+AGG	-	chr5.1:74864349-74864368	MS.gene94654:intron	45.0%
	GGAAAAGGCCTTTGTCTTGT+AGG	+	chr5.1:74864862-74864881	None:intergenic	45.0%
	GGACTGACATTAGCAGGAAA+AGG	+	chr5.1:74864877-74864896	None:intergenic	45.0%
	GGAGTCATGCCAATTGTAGA+CGG	-	chr5.1:74866293-74866312	MS.gene94654:CDS	45.0%
	GGCCTTTGTCTTGTAGGTAT+AGG	+	chr5.1:74864856-74864875	None:intergenic	45.0%
	GGTCCAATTATGTATGTGGG+AGG	-	chr5.1:74865327-74865346	MS.gene94654:CDS	45.0%
	TCCCCTCCCACATACATAAT+TGG	+	chr5.1:74865333-74865352	None:intergenic	45.0%
	TCCTCTAGTGCTTGGTTTAG+AGG	-	chr5.1:74865125-74865144	MS.gene94654:CDS	45.0%
	TCGAAGGCTATCCGTTTACT+TGG	-	chr5.1:74865960-74865979	MS.gene94654:CDS	45.0%
	TGACAACTACAACCTTGTGG+TGG	-	chr5.1:74864384-74864403	MS.gene94654:intron	45.0%
	TGATCACTATCCCATCTTGC+TGG	-	chr5.1:74866102-74866121	MS.gene94654:CDS	45.0%
	TTAGACTCATTCTCCTTGGC+AGG	+	chr5.1:74865427-74865446	None:intergenic	45.0%
	TTATCTGATGTTCCGGTCGA+AGG	-	chr5.1:74865944-74865963	MS.gene94654:CDS	45.0%
	TTCCATGATCTGGACATCAG+CGG	+	chr5.1:74864176-74864195	None:intergenic	45.0%
	TTCCTGCTAATGTCAGTCCA+GGG	-	chr5.1:74864878-74864897	MS.gene94654:intron	45.0%
	TTGACAACGTCGTTGAAACC+CGG	+	chr5.1:74866199-74866218	None:intergenic	45.0%
	TTTCCGAGACCGTCTACAAT+TGG	+	chr5.1:74866305-74866324	None:intergenic	45.0%
	TTTCCTGCTAATGTCAGTCC+AGG	-	chr5.1:74864877-74864896	MS.gene94654:intron	45.0%
!	ATGCAACTACTTGCTAAGGC+GGG	-	chr5.1:74865024-74865043	MS.gene94654:intron	45.0%
!	CAGGTCCATAACTTGCCATT+TGG	-	chr5.1:74864469-74864488	MS.gene94654:intron	45.0%
!	CCCATCAATCTAGGATTCCA+TGG	-	chr5.1:74865810-74865829	MS.gene94654:CDS	45.0%
!	CGCTCGAATTGGCTTATCAA+TGG	-	chr5.1:74865893-74865912	MS.gene94654:CDS	45.0%
!	GATTGTGTTGGTAGAAGTGG+CGG	-	chr5.1:74865614-74865633	MS.gene94654:CDS	45.0%
!	GGATAGCCGGCTTATAATAC+TGG	+	chr5.1:74865511-74865530	None:intergenic	45.0%
!	TTTGAGATGGAGCAGGATGA+CGG	-	chr5.1:74864751-74864770	MS.gene94654:intron	45.0%
!!	ATTTTCAAGCAGCAGCTGTG+TGG	+	chr5.1:74865094-74865113	None:intergenic	45.0%
	AAGTTCTCGATGAAGGTCCG+TGG	-	chr5.1:74864356-74864375	MS.gene94654:intron	50.0%
	ACAGTCGCAGTAACAGTACC+AGG	+	chr5.1:74864421-74864440	None:intergenic	50.0%
	AGCAGCTGTGTGGAAGATGA+GGG	+	chr5.1:74865084-74865103	None:intergenic	50.0%
	ATGGGATAGTGATCAGAGGC+AGG	+	chr5.1:74866097-74866116	None:intergenic	50.0%
	CCATCTTCGCAAGGAAATGC+AGG	-	chr5.1:74865153-74865172	MS.gene94654:CDS	50.0%
	CCCTTCGAGGTTCAAATCAG+CGG	+	chr5.1:74864107-74864126	None:intergenic	50.0%
	CCTGCATTTCCTTGCGAAGA+TGG	+	chr5.1:74865156-74865175	None:intergenic	50.0%
	CTTTAGCAGCATCAAGTCGG+TGG	+	chr5.1:74864339-74864358	None:intergenic	50.0%
	GCCTCTAAACCAAGCACTAG+AGG	+	chr5.1:74865129-74865148	None:intergenic	50.0%
	GCGTACTTTCAGACGCATGT+AGG	+	chr5.1:74864581-74864600	None:intergenic	50.0%
	GGACCTGAACCCAAATGTCA+AGG	+	chr5.1:74864456-74864475	None:intergenic	50.0%
	GGAGTACAGTCCAGCAAGAT+GGG	+	chr5.1:74866115-74866134	None:intergenic	50.0%
	GGGTTGACCTTGTCTGATGA+AGG	-	chr5.1:74864124-74864143	MS.gene94654:CDS	50.0%
	GTAAACGGATAGCCTTCGAC+CGG	+	chr5.1:74865959-74865978	None:intergenic	50.0%
	GTCTAGTGTAGGAAGGGTTG+CGG	-	chr5.1:74865290-74865309	MS.gene94654:CDS	50.0%
	GTTGCGGGTAGCAACAATGT+AGG	-	chr5.1:74865306-74865325	MS.gene94654:CDS	50.0%
	TCAGGCAGTGTTCCATGATC+TGG	+	chr5.1:74864186-74864205	None:intergenic	50.0%
	TCCTGCTAATGTCAGTCCAG+GGG	-	chr5.1:74864879-74864898	MS.gene94654:intron	50.0%
	TCTAGTGTAGGAAGGGTTGC+GGG	-	chr5.1:74865291-74865310	MS.gene94654:CDS	50.0%
	TCTGAAGATCAAGCCTGCCA+AGG	-	chr5.1:74865411-74865430	MS.gene94654:CDS	50.0%
	TGGAGTACAGTCCAGCAAGA+TGG	+	chr5.1:74866116-74866135	None:intergenic	50.0%
	TGGGATAGTGATCAGAGGCA+GGG	+	chr5.1:74866096-74866115	None:intergenic	50.0%
	TGGTACTGTTACTGCGACTG+TGG	-	chr5.1:74864420-74864439	MS.gene94654:intron	50.0%
	TTGAAGTTGCGGCGATTTCC+AGG	+	chr5.1:74866151-74866170	None:intergenic	50.0%
	TTGGAACGTCTTCACCGTTG+TGG	-	chr5.1:74866253-74866272	MS.gene94654:CDS	50.0%
!	AATTCTGATGAGACGGGCTC+TGG	-	chr5.1:74865189-74865208	MS.gene94654:CDS	50.0%
!	ATTCTGATGAGACGGGCTCT+GGG	-	chr5.1:74865190-74865209	MS.gene94654:CDS	50.0%
!	CGAGGCTGGATTAGACCAAA+TGG	+	chr5.1:74864487-74864506	None:intergenic	50.0%
!	GAGCTCTAGCAACTACTGGT+TGG	-	chr5.1:74866029-74866048	MS.gene94654:CDS	50.0%
!	TCATCCAGGCTGATGACGAT+CGG	-	chr5.1:74864296-74864315	MS.gene94654:intron	50.0%
!!	CCGCTGATTTGAACCTCGAA+GGG	-	chr5.1:74864104-74864123	MS.gene94654:CDS	50.0%
	AAAGGCCCATGGCGGTTAAC+AGG	-	chr5.1:74865725-74865744	MS.gene94654:CDS	55.0%
	AAATGTACCCCATGCGCTCC+CGG	+	chr5.1:74864252-74864271	None:intergenic	55.0%
	ACCCCTGGACTGACATTAGC+AGG	+	chr5.1:74864883-74864902	None:intergenic	55.0%
	ACGTCTTCACCGTTGTGGAG+AGG	-	chr5.1:74866258-74866277	MS.gene94654:CDS	55.0%
	AGACGTTCCTCAACCACACG+CGG	+	chr5.1:74866004-74866023	None:intergenic	55.0%
	AGCCGCTGATGTCCAGATCA+TGG	-	chr5.1:74864171-74864190	MS.gene94654:CDS	55.0%
	AGGGGCAGCCAAGTTCACAA+GGG	+	chr5.1:74866077-74866096	None:intergenic	55.0%
	ATCTGGACATCAGCGGCTAC+AGG	+	chr5.1:74864169-74864188	None:intergenic	55.0%
	CAACACCGTCCTCTAGTGCT+TGG	-	chr5.1:74865117-74865136	MS.gene94654:CDS	55.0%
	CAGACAAGGTCAACCCTTCG+AGG	+	chr5.1:74864120-74864139	None:intergenic	55.0%
	CAGCAGCTGTGTGGAAGATG+AGG	+	chr5.1:74865085-74865104	None:intergenic	55.0%
	CATCAAGTCGGTGGCGAAAC+TGG	+	chr5.1:74864330-74864349	None:intergenic	55.0%
	CATCAGCGGCTACAGGATCT+AGG	+	chr5.1:74864162-74864181	None:intergenic	55.0%
	GAAACCGATCGTCATCAGCC+TGG	+	chr5.1:74864303-74864322	None:intergenic	55.0%
	GCATGTTGTTGTGACACCCC+TGG	+	chr5.1:74864898-74864917	None:intergenic	55.0%
	GGGATAGTGATCAGAGGCAG+GGG	+	chr5.1:74866095-74866114	None:intergenic	55.0%
	GGGTGAATGGCTTCGTCCTA+CGG	-	chr5.1:74864786-74864805	MS.gene94654:intron	55.0%
	GGTCTAATCCAGCCTCGTAC+CGG	-	chr5.1:74864490-74864509	MS.gene94654:intron	55.0%
!	CTTATTTCCGGGAGCGCATG+GGG	-	chr5.1:74864242-74864261	MS.gene94654:intron	55.0%
!	GACCGAGCTCTAGCAACTAC+TGG	-	chr5.1:74866025-74866044	MS.gene94654:CDS	55.0%
!	GCTTATTTCCGGGAGCGCAT+GGG	-	chr5.1:74864241-74864260	MS.gene94654:intron	55.0%
!	TGCTTATTTCCGGGAGCGCA+TGG	-	chr5.1:74864240-74864259	MS.gene94654:CDS	55.0%
!	TTGTCGCAGCTGTACCGACA+CGG	+	chr5.1:74864816-74864835	None:intergenic	55.0%
!!	GCCGCTGATTTGAACCTCGA+AGG	-	chr5.1:74864103-74864122	MS.gene94654:CDS	55.0%
	AGATGGAATTGCCGGGGAGC+AGG	-	chr5.1:74865449-74865468	MS.gene94654:CDS	60.0%
	CAGGGGCAGCCAAGTTCACA+AGG	+	chr5.1:74866078-74866097	None:intergenic	60.0%
	CCAATACCGTGACCGGTACG+AGG	+	chr5.1:74864505-74864524	None:intergenic	60.0%
	GCGGGTAAGAACAACGCTGC+TGG	-	chr5.1:74865042-74865061	MS.gene94654:intron	60.0%
	GTGCGATCCGTACCACCACA+AGG	+	chr5.1:74864399-74864418	None:intergenic	60.0%
	TTGCAAGTGCGGGTGCGTGA+CGG	-	chr5.1:74864631-74864650	MS.gene94654:intron	60.0%
!	ATCCAGCCTCGTACCGGTCA+CGG	-	chr5.1:74864496-74864515	MS.gene94654:intron	60.0%
!	CCTCGTACCGGTCACGGTAT+TGG	-	chr5.1:74864502-74864521	MS.gene94654:intron	60.0%
!	GGGAGCGCATGGGGTACATT+TGG	-	chr5.1:74864251-74864270	MS.gene94654:intron	60.0%
	AAGCCTTGGCACACCGCGTG+TGG	-	chr5.1:74865988-74866007	MS.gene94654:CDS	65.0%
	CAACCACACGCGGTGTGCCA+AGG	+	chr5.1:74865994-74866013	None:intergenic	65.0%
	CGTCCTACGGTTCGCCGTGT+CGG	-	chr5.1:74864799-74864818	MS.gene94654:intron	65.0%
	CTCGTCGACGACCCCCGTTT+GGG	+	chr5.1:74865763-74865782	None:intergenic	65.0%
	GAAGCCATTCACCCCAGCCG+CGG	+	chr5.1:74864780-74864799	None:intergenic	65.0%
	GAGGCAGCGCCATCTTCGCA+AGG	-	chr5.1:74865144-74865163	MS.gene94654:CDS	65.0%
	GTGGTGGTACGGATCGCACC+TGG	-	chr5.1:74864400-74864419	MS.gene94654:intron	65.0%
	TACCGTGACCGGTACGAGGC+TGG	+	chr5.1:74864501-74864520	None:intergenic	65.0%
	TGGCACACCGCGTGTGGTTG+AGG	-	chr5.1:74865994-74866013	MS.gene94654:CDS	65.0%
!	GAGCAGGATGACGGTCACCG+CGG	-	chr5.1:74864760-74864779	MS.gene94654:intron	65.0%
!	GTACCGACACGGCGAACCGT+AGG	+	chr5.1:74864805-74864824	None:intergenic	65.0%
!!	TGATGAGACGGGCTCTGGGC+TGG	-	chr5.1:74865194-74865213	MS.gene94654:CDS	65.0%
	CACTCGGGCTACCTGCTCCC+CGG	+	chr5.1:74865463-74865482	None:intergenic	70.0%
	GCTCGTCGACGACCCCCGTT+TGG	+	chr5.1:74865764-74865783	None:intergenic	70.0%
	GTCACCGCGGCTGGGGTGAA+TGG	-	chr5.1:74864773-74864792	MS.gene94654:intron	70.0%
!	AGGATGACGGTCACCGCGGC+TGG	-	chr5.1:74864764-74864783	MS.gene94654:intron	70.0%
!	GATGACGGTCACCGCGGCTG+GGG	-	chr5.1:74864766-74864785	MS.gene94654:intron	70.0%
!	GGATGACGGTCACCGCGGCT+GGG	-	chr5.1:74864765-74864784	MS.gene94654:intron	70.0%
!	GGGTCGTCGACGAGCGTCGT+GGG	-	chr5.1:74865769-74865788	MS.gene94654:CDS	70.0%
!	GGGGTCGTCGACGAGCGTCG+TGG	-	chr5.1:74865768-74865787	MS.gene94654:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	74864100	74866331	74864100	ID=MS.gene94654
chr5.1	mRNA	74864100	74866331	74864100	ID=MS.gene94654.t1;Parent=MS.gene94654
chr5.1	exon	74865049	74866331	74865049	ID=MS.gene94654.t1.exon1;Parent=MS.gene94654.t1
chr5.1	CDS	74865049	74866331	74865049	ID=cds.MS.gene94654.t1;Parent=MS.gene94654.t1
chr5.1	exon	74864100	74864259	74864100	ID=MS.gene94654.t1.exon2;Parent=MS.gene94654.t1
chr5.1	CDS	74864100	74864259	74864100	ID=cds.MS.gene94654.t1;Parent=MS.gene94654.t1

Gene Sequence

>MS.gene94654

ATGGCCGCTGATTTGAACCTCGAAGGGTTGACCTTGTCTGATGAAGGTTTAACTCTAGACCTAGATCCTGTAGCCGCTGATGTCCAGATCATGGAACACTGCCTGATTGGTCGTTTGTTATCTGACAAACAGATCAAAACTGCTTATTTCCGGGAGCGCATGGGGTACATTTGGAGACCAGTCAAGAAAGTCTCTATCATCCAGGCTGATGACGATCGGTTTCTATTCCAGTTTCGCCACCGACTTGATGCTGCTAAAGTTCTCGATGAAGGTCCGTGGTTGTATGACAACTACAACCTTGTGGTGGTACGGATCGCACCTGGTACTGTTACTGCGACTGTGGATCTGAATTTCCTTGACATTTGGGTTCAGGTCCATAACTTGCCATTTGGTCTAATCCAGCCTCGTACCGGTCACGGTATTGGTCATTTTCACGGAGAGCTGAAAGTATACGATGACAGAAACACTCTGCACAGCTCCTACATGCGTCTGAAAGTACGCATAAATGTCACAGAACCTTTGAAAAATGAATTGCAAGTGCGGGTGCGTGACGGTAGTTATGTTACTTGCTATTTTAAGTATGAAAAGCTTGGTGATTTTTGTTACATGTGTGGTATACTTGGTCACACTGACAAGTCATGTCCTAGATTGTTTGAGATGGAGCAGGATGACGGTCACCGCGGCTGGGGTGAATGGCTTCGTCCTACGGTTCGCCGTGTCGGTACAGCTGCGACAAATAGATATTTGAATGACCCTATACCTACAAGACAAAGGCCTTTTCCTGCTAATGTCAGTCCAGGGGTGTCACAACAACATGCTTCAGTTTCAACTTCGGCGACTCCGCAAATGAACACTTATGTTGGTAGAATATCTGCTGTTCAACAGGAAATTCATGCGATTAAGTCTGGGTTATTGACGGCCCAAATGCAACTACTTGCTAAGGCGGGTAAGAACAACGCTGCTGGTTCTTCTCTACATCTTCCCTCATCTTCCACACAGCTGCTGCTTGAAAATGCTCAACACCGTCCTCTAGTGCTTGGTTTAGAGGCAGCGCCATCTTCGCAAGGAAATGCAGGTAGCTCGAATGACAATTCTGATGAGACGGGCTCTGGGCTGGAATTGAAAAAGAGAAAGCGTTCCAAGAATGTGCAACAATCTACTGCAGGATTTCAGTCAAGTCATGAAGATGTGTCTAGTGTAGGAAGGGTTGCGGGTAGCAACAATGTAGGTCCAATTATGTATGTGGGAGGGGATGTTGAGATGTCTGTAATGGATAATCCTTTGTATGCTGATCCTAATGTAAAGGCAGGTTCTGAAGATCAAGCCTGCCAAGGAGAATGAGTCTAATTAGATGGAATTGCCGGGGAGCAGGTAGCCCGAGTGCAATTCCTCATTTGAAGTACCTAACCCAGTATTATAAGCCGGCTATCCTTTTTCTTAGCGAGACACTAGTCTGCCGTAATAAAATAGAGGAACTTCGTTATTTTCTCGGCTTTGATTTTTCTTTTTCTGTTGATTGTGTTGGTAGAAGTGGCGGTTTAGCTTTATTTTGGAATTCTTATATTAATTGCAAACTCTTAGACTATTCACAAAATCATATATCTGTTGAAATTAATGATTGTGAAAAAGGCCCATGGCGGTTAACAGGTTTTTATGGTTACCCAAACGGGGGTCGTCGACGAGCGTCGTGGGATTTGATACGTCAATTATCCCATCAATCTAGGATTCCATGGTGCATTTTCGGTGATTTTAACGACATCATGGATGCAAGTGAAAAAAGGGGACGAAATCTTCGCTCGAATTGGCTTATCAATGGCTTCAGGAAAGCTGTGTTAGATTCTGGTTTATCTGATGTTCCGGTCGAAGGCTATCCGTTTACTTGGTTCAAAAGCCTTGGCACACCGCGTGTGGTTGAGGAACGTCTCGACCGAGCTCTAGCAACTACTGGTTGGTTTGATTTTTTTCCTAATTCTTCCCTTGTGAACTTGGCTGCCCCTGCCTCTGATCACTATCCCATCTTGCTGGACTGTACTCCAATTGTTAGAGCTCCTGGAAATCGCCGCAACTTCAAGTTTGAAAATGAGTGGAAATTGGAACCGGGTTTCAACGACGTTGTCAAAGAAAGTTGGTCCGCATACACATCTGAACCGATTTTGGAACGTCTTCACCGTTGTGGAGAGGATATGTTTCATTGGAGTCATGCCAATTGTAGACGGTCTCGGAAAGATTTAG

Protein sequence

>MS.gene94654.t1

MAADLNLEGLTLSDEGLTLDLDPVAADVQIMEHCLIGRLLSDKQIKTAYFRERMGYIWRPVKKVSIIQADDDRFLFQFRHRLDAAKVLDEGPWLYDNYNLVVVRIAPGTVTATVDLNFLDIWVQVHNLPFGLIQPRTGHGIGHFHGELKVYDDRNTLHSSYMRLKVRINVTEPLKNELQVRVRDGSYVTCYFKYEKLGDFCYMCGILGHTDKSCPRLFEMEQDDGHRGWGEWLRPTVRRVGTAATNRYLNDPIPTRQRPFPANVSPGVSQQHASVSTSATPQMNTYVGRISAVQQEIHAIKSGLLTAQMQLLAKAGKNNAAGSSLHLPSSSTQLLLENAQHRPLVLGLEAAPSSQGNAGSSNDNSDETGSGLELKKRKRSKNVQQSTAGFQSSHEDVSSVGRVAGSNNVGPIMYVGGDVEMSVMDNPFLKMSGNWNRVSTTLSKKVGPHTHLNRFWNVFTVVERICFIGVMPIVDGLGKI