AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042614


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042614.t1 MTR_3g053920 48.661 224 74 3 1 209 83 280 4.18e-52 171
MS.gene042614.t1 MTR_8g463110 66.667 120 40 0 152 271 1 120 1.25e-38 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGCTTCTAATTTGATTAC+TGG 0.270991 5.2:-46962959 MS.gene042614:CDS
CATTGGCAGCGCCACTTGTA+TGG 0.320012 5.2:+46963140 None:intergenic
GACTCCTCAGAGTTAGCATT+TGG 0.346452 5.2:+46962899 None:intergenic
GGTGAAGGAAATCTTGTAAT+AGG 0.354095 5.2:-46962788 MS.gene042614:CDS
CGCGGCACAGCAGAGGGTAT+TGG 0.359383 5.2:+46963205 None:intergenic
GCGGCACAGCAGAGGGTATT+GGG 0.376991 5.2:+46963206 None:intergenic
TCAACGATGGTGTACGCAAT+TGG 0.382810 5.2:-46963294 MS.gene042614:CDS
TTTGATTACTGGTTGTCCTT+TGG 0.383115 5.2:-46962948 MS.gene042614:CDS
AATTCCAACTCGAACAAATC+CGG 0.401725 5.2:+46963316 None:intergenic
ACTGGTTGTCCTTTGGTGCT+CGG 0.402188 5.2:-46962941 MS.gene042614:CDS
GAAGAAGCAGGCTTCAAGTC+AGG 0.403741 5.2:+46963268 None:intergenic
AATTGAGTTACGGACATCAT+AGG 0.412616 5.2:+46963506 None:intergenic
GCCAAAGCAAAACATGGTTC+TGG 0.415532 5.2:-46963034 MS.gene042614:CDS
TATTAGCAGCAGAACGAATA+CGG 0.441239 5.2:+46963245 None:intergenic
CAACGATGGTGTACGCAATT+GGG 0.442605 5.2:-46963293 MS.gene042614:CDS
CAGGGTATAAATAGCAATCT+AGG 0.447039 5.2:-46963540 MS.gene042614:CDS
GCCACTGCTCCTTTGAAGAA+TGG 0.449021 5.2:-46963447 MS.gene042614:CDS
TTGGTGCTCGGATTACCTGC+TGG 0.450819 5.2:-46962929 MS.gene042614:CDS
GTTCTGCTGCTAATAACAAA+TGG 0.473309 5.2:-46963237 MS.gene042614:CDS
TGTTACAGGTGTGGTTTGCT+TGG 0.473815 5.2:-46963358 MS.gene042614:intron
AGGATGATGGCTCTTCATAA+TGG 0.484877 5.2:-46962839 MS.gene042614:CDS
ACGGACTTTCAACCGCATAT+AGG 0.485165 5.2:+46963476 None:intergenic
GAGGAGTCTCAAGATGCTAC+TGG 0.487924 5.2:-46962884 MS.gene042614:CDS
CTACATCATTTGCTGCTGAT+GGG 0.487952 5.2:-46962811 MS.gene042614:CDS
ACTACATCATTTGCTGCTGA+TGG 0.504564 5.2:-46962812 MS.gene042614:CDS
GCATTTGGAAACGGTCCAGC+AGG 0.514131 5.2:+46962914 None:intergenic
TTCTTCTTCCACAGGTACCT+CGG 0.517778 5.2:-46962978 MS.gene042614:CDS
ATTGGCAGCGCCACTTGTAT+GGG 0.521231 5.2:+46963141 None:intergenic
ACAGACTTTCCATTCTTCAA+AGG 0.539193 5.2:+46963438 None:intergenic
AGGTACTGTGAATTGAGTTA+CGG 0.541155 5.2:+46963496 None:intergenic
TTGCCCGGATTTGTTCGAGT+TGG 0.543523 5.2:-46963320 MS.gene042614:CDS
ACACACAGACAAAGTTTGCC+CGG 0.544658 5.2:-46963335 MS.gene042614:CDS
TACATCATTTGCTGCTGATG+GGG 0.545658 5.2:-46962810 MS.gene042614:CDS
ATTCCAACTCGAACAAATCC+GGG 0.547472 5.2:+46963317 None:intergenic
TCAGAGTTAGCATTTGGAAA+CGG 0.549711 5.2:+46962905 None:intergenic
AAGGAGCAGTGGCATTGATA+CGG 0.550294 5.2:+46963457 None:intergenic
ATTAGCAGCAGAACGAATAC+GGG 0.553476 5.2:+46963246 None:intergenic
TAGGAACAAATGTTAGTCGT+GGG 0.555722 5.2:-46962769 MS.gene042614:CDS
TCCATTCTTCAAAGGAGCAG+TGG 0.556637 5.2:+46963446 None:intergenic
CCTGCTGGTGTGTCTCATGT+TGG 0.558690 5.2:-46963172 MS.gene042614:CDS
GAACGAATACGGGAAGAAGC+AGG 0.560605 5.2:+46963256 None:intergenic
GAGGTACCTGTGGAAGAAGA+AGG 0.563247 5.2:+46962980 None:intergenic
CCGCGCCACCATGTACCTGC+TGG 0.566092 5.2:-46963187 MS.gene042614:CDS
AAGAAGCAGGCTTCAAGTCA+GGG 0.569130 5.2:+46963269 None:intergenic
GTCACTGAAAGTTACAGCAT+TGG 0.571105 5.2:+46963123 None:intergenic
TGGTGGCGCGGCACAGCAGA+GGG 0.580282 5.2:+46963199 None:intergenic
ATGGTGGCGCGGCACAGCAG+AGG 0.586786 5.2:+46963198 None:intergenic
GTCATGGATCGTATCCAACC+TGG 0.588594 5.2:-46963654 MS.gene042614:CDS
TTTCAGTGACTGTATGCTTG+CGG 0.599794 5.2:-46963110 MS.gene042614:CDS
CCAACATGAGACACACCAGC+AGG 0.601219 5.2:+46963172 None:intergenic
ATAGGAACAAATGTTAGTCG+TGG 0.601283 5.2:-46962770 MS.gene042614:CDS
GAGACACACCAGCAGGTACA+TGG 0.604839 5.2:+46963179 None:intergenic
TGAAAATTATCGTACAGCCA+TGG 0.609399 5.2:+46963681 None:intergenic
AACATTGCCAAAGCAAAACA+TGG 0.613835 5.2:-46963040 MS.gene042614:CDS
CCAGCAGGTACATGGTGGCG+CGG 0.614905 5.2:+46963187 None:intergenic
GGAACAAATGTTAGTCGTGG+GGG 0.620199 5.2:-46962767 MS.gene042614:CDS
ATTAGAAGCCGAGGTACCTG+TGG 0.621664 5.2:+46962970 None:intergenic
ATCTAGGGACGACACCAGGT+TGG 0.621826 5.2:+46963640 None:intergenic
AGTAATCAAATTAGAAGCCG+AGG 0.623628 5.2:+46962961 None:intergenic
TTCGAGTTGGAATTCAACGA+TGG 0.626874 5.2:-46963307 MS.gene042614:CDS
ACATCATTTGCTGCTGATGG+GGG 0.646184 5.2:-46962809 MS.gene042614:CDS
AGGAACAAATGTTAGTCGTG+GGG 0.646490 5.2:-46962768 MS.gene042614:CDS
GCAGGTAATCCGAGCACCAA+AGG 0.647768 5.2:+46962932 None:intergenic
ATAGATCTAGGGACGACACC+AGG 0.648846 5.2:+46963636 None:intergenic
GGTCGAAACTCCCATACAAG+TGG 0.654131 5.2:-46963151 MS.gene042614:CDS
GAACAAATGTTAGTCGTGGG+GGG 0.666053 5.2:-46962766 MS.gene042614:CDS
CAATTCACAGTACCTATATG+CGG 0.706438 5.2:-46963488 MS.gene042614:CDS
GTTTCCAAATGCTAACTCTG+AGG 0.709040 5.2:-46962903 MS.gene042614:CDS
ACACACCAGCAGGTACATGG+TGG 0.724022 5.2:+46963182 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGGATCGTCATTAAAAATA+CGG + chr5.2:46962768-46962787 None:intergenic 25.0%
! CTGAATTAAAAAAGAGAAAG+AGG - chr5.2:46963606-46963625 MS.gene042614:intron 25.0%
AAAAAAGAGAAAGAGGATGA+TGG - chr5.2:46963613-46963632 MS.gene042614:intron 30.0%
TGGATCGTCATTAAAAATAC+GGG + chr5.2:46962767-46962786 None:intergenic 30.0%
! CGATAATTTTCATATCGTCA+TGG - chr5.2:46962795-46962814 MS.gene042614:CDS 30.0%
! TTTTGCTTTGGCAATGTTTT+GGG + chr5.2:46963423-46963442 None:intergenic 30.0%
!! GTATTTTTAATGACGATCCA+TGG - chr5.2:46962767-46962786 MS.gene042614:CDS 30.0%
!!! AACTCGTAGTTTTTTGTTAC+AGG - chr5.2:46963093-46963112 MS.gene042614:CDS 30.0%
AACATTGCCAAAGCAAAACA+TGG - chr5.2:46963425-46963444 MS.gene042614:intron 35.0%
AATTCCAACTCGAACAAATC+CGG + chr5.2:46963152-46963171 None:intergenic 35.0%
AATTGAGTTACGGACATCAT+AGG + chr5.2:46962962-46962981 None:intergenic 35.0%
ACAGACTTTCCATTCTTCAA+AGG + chr5.2:46963030-46963049 None:intergenic 35.0%
AGTAATCAAATTAGAAGCCG+AGG + chr5.2:46963507-46963526 None:intergenic 35.0%
ATAGGAACAAATGTTAGTCG+TGG - chr5.2:46963695-46963714 MS.gene042614:CDS 35.0%
ATTCGGTTGAATATAGCCAA+AGG + chr5.2:46962899-46962918 None:intergenic 35.0%
CAATTCACAGTACCTATATG+CGG - chr5.2:46962977-46962996 MS.gene042614:CDS 35.0%
CAGGGTATAAATAGCAATCT+AGG - chr5.2:46962925-46962944 MS.gene042614:CDS 35.0%
GGTGAAGGAAATCTTGTAAT+AGG - chr5.2:46963677-46963696 MS.gene042614:CDS 35.0%
GTTCTGCTGCTAATAACAAA+TGG - chr5.2:46963228-46963247 MS.gene042614:CDS 35.0%
TAGGAACAAATGTTAGTCGT+GGG - chr5.2:46963696-46963715 MS.gene042614:CDS 35.0%
TATTAGCAGCAGAACGAATA+CGG + chr5.2:46963223-46963242 None:intergenic 35.0%
TCAGAGTTAGCATTTGGAAA+CGG + chr5.2:46963563-46963582 None:intergenic 35.0%
TCGGCTTCTAATTTGATTAC+TGG - chr5.2:46963506-46963525 MS.gene042614:CDS 35.0%
TGAAAATTATCGTACAGCCA+TGG + chr5.2:46962787-46962806 None:intergenic 35.0%
TGTAACTTAAGCACCATTAC+TGG + chr5.2:46963054-46963073 None:intergenic 35.0%
TTTATACCCTGACCAACATT+CGG + chr5.2:46962916-46962935 None:intergenic 35.0%
! AGGTACTGTGAATTGAGTTA+CGG + chr5.2:46962972-46962991 None:intergenic 35.0%
! GTTTTGCTTTGGCAATGTTT+TGG + chr5.2:46963424-46963443 None:intergenic 35.0%
! TTTGATTACTGGTTGTCCTT+TGG - chr5.2:46963517-46963536 MS.gene042614:CDS 35.0%
! TTTTTACCTTCTTCTTCCAC+AGG - chr5.2:46963479-46963498 MS.gene042614:CDS 35.0%
!!! GTAGTTTTTTGTTACAGGTG+TGG - chr5.2:46963098-46963117 MS.gene042614:CDS 35.0%
AGGAACAAATGTTAGTCGTG+GGG - chr5.2:46963697-46963716 MS.gene042614:CDS 40.0%
ATCTATCGACCTGAACCATT+TGG - chr5.2:46962846-46962865 MS.gene042614:CDS 40.0%
ATTAGCAGCAGAACGAATAC+GGG + chr5.2:46963222-46963241 None:intergenic 40.0%
ATTCAACCGAATGTTGGTCA+GGG - chr5.2:46962907-46962926 MS.gene042614:CDS 40.0%
ATTCCAACTCGAACAAATCC+GGG + chr5.2:46963151-46963170 None:intergenic 40.0%
CCATTTGGACATCTAGGTTT+AGG - chr5.2:46962861-46962880 MS.gene042614:CDS 40.0%
CCTAAACCTAGATGTCCAAA+TGG + chr5.2:46962864-46962883 None:intergenic 40.0%
GGCTATATTCAACCGAATGT+TGG - chr5.2:46962901-46962920 MS.gene042614:CDS 40.0%
GTCACTGAAAGTTACAGCAT+TGG + chr5.2:46963345-46963364 None:intergenic 40.0%
GTTTCCAAATGCTAACTCTG+AGG - chr5.2:46963562-46963581 MS.gene042614:intron 40.0%
TATTCAACCGAATGTTGGTC+AGG - chr5.2:46962906-46962925 MS.gene042614:CDS 40.0%
TTTCAGTGACTGTATGCTTG+CGG - chr5.2:46963355-46963374 MS.gene042614:intron 40.0%
! ACCAGAACCATGTTTTGCTT+TGG + chr5.2:46963435-46963454 None:intergenic 40.0%
! ACTACATCATTTGCTGCTGA+TGG - chr5.2:46963653-46963672 MS.gene042614:CDS 40.0%
! AGGATGATGGCTCTTCATAA+TGG - chr5.2:46963626-46963645 MS.gene042614:intron 40.0%
! CTACATCATTTGCTGCTGAT+GGG - chr5.2:46963654-46963673 MS.gene042614:CDS 40.0%
! TACATCATTTGCTGCTGATG+GGG - chr5.2:46963655-46963674 MS.gene042614:CDS 40.0%
!! TTCGAGTTGGAATTCAACGA+TGG - chr5.2:46963158-46963177 MS.gene042614:CDS 40.0%
AAGAAGCAGGCTTCAAGTCA+GGG + chr5.2:46963199-46963218 None:intergenic 45.0%
ACACACAGACAAAGTTTGCC+CGG - chr5.2:46963130-46963149 MS.gene042614:CDS 45.0%
ACGGACTTTCAACCGCATAT+AGG + chr5.2:46962992-46963011 None:intergenic 45.0%
CAACGATGGTGTACGCAATT+GGG - chr5.2:46963172-46963191 MS.gene042614:CDS 45.0%
CCTAGATGTCCAAATGGTTC+AGG + chr5.2:46962858-46962877 None:intergenic 45.0%
CCTGAACCATTTGGACATCT+AGG - chr5.2:46962855-46962874 MS.gene042614:CDS 45.0%
GAACAAATGTTAGTCGTGGG+GGG - chr5.2:46963699-46963718 MS.gene042614:CDS 45.0%
GACTCCTCAGAGTTAGCATT+TGG + chr5.2:46963569-46963588 None:intergenic 45.0%
GCCAAAGCAAAACATGGTTC+TGG - chr5.2:46963431-46963450 MS.gene042614:intron 45.0%
GGAACAAATGTTAGTCGTGG+GGG - chr5.2:46963698-46963717 MS.gene042614:CDS 45.0%
GGTTCAGGTCGATAGATCTA+GGG + chr5.2:46962843-46962862 None:intergenic 45.0%
TAGGTTCACGATCTCTCCTT+TGG - chr5.2:46962880-46962899 MS.gene042614:CDS 45.0%
TCAACGATGGTGTACGCAAT+TGG - chr5.2:46963171-46963190 MS.gene042614:CDS 45.0%
TCCATTCTTCAAAGGAGCAG+TGG + chr5.2:46963022-46963041 None:intergenic 45.0%
TGGAAAGTCTGTGCCAGTAA+TGG - chr5.2:46963038-46963057 MS.gene042614:CDS 45.0%
TGGTTCAGGTCGATAGATCT+AGG + chr5.2:46962844-46962863 None:intergenic 45.0%
TTCTTCTTCCACAGGTACCT+CGG - chr5.2:46963487-46963506 MS.gene042614:CDS 45.0%
! ACATCATTTGCTGCTGATGG+GGG - chr5.2:46963656-46963675 MS.gene042614:CDS 45.0%
!! AAGGAGCAGTGGCATTGATA+CGG + chr5.2:46963011-46963030 None:intergenic 45.0%
!! TGTTACAGGTGTGGTTTGCT+TGG - chr5.2:46963107-46963126 MS.gene042614:CDS 45.0%
ATAGATCTAGGGACGACACC+AGG + chr5.2:46962832-46962851 None:intergenic 50.0%
ATTGGCAGCGCCACTTGTAT+GGG + chr5.2:46963327-46963346 None:intergenic 50.0%
GAACGAATACGGGAAGAAGC+AGG + chr5.2:46963212-46963231 None:intergenic 50.0%
GAAGAAGCAGGCTTCAAGTC+AGG + chr5.2:46963200-46963219 None:intergenic 50.0%
GAGGAGTCTCAAGATGCTAC+TGG - chr5.2:46963581-46963600 MS.gene042614:intron 50.0%
GGTCGAAACTCCCATACAAG+TGG - chr5.2:46963314-46963333 MS.gene042614:CDS 50.0%
GTCATGGATCGTATCCAACC+TGG - chr5.2:46962811-46962830 MS.gene042614:CDS 50.0%
! ATTAGAAGCCGAGGTACCTG+TGG + chr5.2:46963498-46963517 None:intergenic 50.0%
! GAGGTACCTGTGGAAGAAGA+AGG + chr5.2:46963488-46963507 None:intergenic 50.0%
! GCCACTGCTCCTTTGAAGAA+TGG - chr5.2:46963018-46963037 MS.gene042614:CDS 50.0%
! TTGCCCGGATTTGTTCGAGT+TGG - chr5.2:46963145-46963164 MS.gene042614:CDS 50.0%
!! ACTGGTTGTCCTTTGGTGCT+CGG - chr5.2:46963524-46963543 MS.gene042614:CDS 50.0%
ACACACCAGCAGGTACATGG+TGG + chr5.2:46963286-46963305 None:intergenic 55.0%
ATCTAGGGACGACACCAGGT+TGG + chr5.2:46962828-46962847 None:intergenic 55.0%
CATTGGCAGCGCCACTTGTA+TGG + chr5.2:46963328-46963347 None:intergenic 55.0%
CCAACATGAGACACACCAGC+AGG + chr5.2:46963296-46963315 None:intergenic 55.0%
CCTGCTGGTGTGTCTCATGT+TGG - chr5.2:46963293-46963312 MS.gene042614:CDS 55.0%
GAGACACACCAGCAGGTACA+TGG + chr5.2:46963289-46963308 None:intergenic 55.0%
GCAGGTAATCCGAGCACCAA+AGG + chr5.2:46963536-46963555 None:intergenic 55.0%
GCATTTGGAAACGGTCCAGC+AGG + chr5.2:46963554-46963573 None:intergenic 55.0%
TTTGCTGCTGATGGGGGTGA+AGG - chr5.2:46963662-46963681 MS.gene042614:CDS 55.0%
! TTGGTGCTCGGATTACCTGC+TGG - chr5.2:46963536-46963555 MS.gene042614:CDS 55.0%
!! GCGGCACAGCAGAGGGTATT+GGG + chr5.2:46963262-46963281 None:intergenic 60.0%
! CCAGCAGGTACATGGTGGCG+CGG + chr5.2:46963281-46963300 None:intergenic 65.0%
! CGCGGCACAGCAGAGGGTAT+TGG + chr5.2:46963263-46963282 None:intergenic 65.0%
CCGCGCCACCATGTACCTGC+TGG - chr5.2:46963278-46963297 MS.gene042614:CDS 70.0%
! ATGGTGGCGCGGCACAGCAG+AGG + chr5.2:46963270-46963289 None:intergenic 70.0%
! TGGTGGCGCGGCACAGCAGA+GGG + chr5.2:46963269-46963288 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 46962754 46963733 46962754 ID=MS.gene042614
chr5.2 mRNA 46962754 46963733 46962754 ID=MS.gene042614.t1;Parent=MS.gene042614
chr5.2 exon 46963650 46963733 46963650 ID=MS.gene042614.t1.exon1;Parent=MS.gene042614.t1
chr5.2 CDS 46963650 46963733 46963650 ID=cds.MS.gene042614.t1;Parent=MS.gene042614.t1
chr5.2 exon 46963444 46963559 46963444 ID=MS.gene042614.t1.exon2;Parent=MS.gene042614.t1
chr5.2 CDS 46963444 46963559 46963444 ID=cds.MS.gene042614.t1;Parent=MS.gene042614.t1
chr5.2 exon 46962754 46963372 46962754 ID=MS.gene042614.t1.exon3;Parent=MS.gene042614.t1
chr5.2 CDS 46962754 46963372 46962754 ID=cds.MS.gene042614.t1;Parent=MS.gene042614.t1
Gene Sequence

>MS.gene042614

ATGGATGCTGCCCGTATTTTTAATGACGATCCATGGCTGTACGATAATTTTCATATCGTCATGGATCGTATCCAACCTGGTGTCGTCCCTAGATCTATCGACCTGAACCATTTGGACATCTAGGTTTAGGTTCACGATCTCTCCTTTGGCTATATTCAACCGAATGTTGGTCAGGGTATAAATAGCAATCTAGGTGAACTCAAATCCTATGATGTCCGTAACTCAATTCACAGTACCTATATGCGGTTGAAAGTCCGTATCAATGCCACTGCTCCTTTGAAGAATGGAAAGTCTGTGCCAGTAATGGTGCTTAAGTTACAGTTAAATTCAAATATGAGAAACTCGTAGTTTTTTGTTACAGGTGTGGTTTGCTTGGACACACAGACAAAGTTTGCCCGGATTTGTTCGAGTTGGAATTCAACGATGGTGTACGCAATTGGGTCCCTGACTTGAAGCCTGCTTCTTCCCGTATTCGTTCTGCTGCTAATAACAAATGGTTGTCAGACCCAATACCCTCTGCTGTGCCGCGCCACCATGTACCTGCTGGTGTGTCTCATGTTGGTCGAAACTCCCATACAAGTGGCGCTGCCAATGCTGTAACTTTCAGTGACTGTATGCTTGCGGTTGACAATCAAATTTCTGCTCTCAAACATGATGTACTTGCTGCCCAAAACATTGCCAAAGCAAAACATGGTTCTGGTGCTAACTTTTCTTGTCAGATGCAATTTTTACCTTCTTCTTCCACAGGTACCTCGGCTTCTAATTTGATTACTGGTTGTCCTTTGGTGCTCGGATTACCTGCTGGACCGTTTCCAAATGCTAACTCTGAGGAGTCTCAAGATGCTACTGGTTCTGAATTAAAAAAGAGAAAGAGGATGATGGCTCTTCATAATGGTGAAACTACATCATTTGCTGCTGATGGGGGTGAAGGAAATCTTGTAATAGGAACAAATGTTAGTCGTGGGGGGATGTTGATATGA

Protein sequence

>MS.gene042614.t1

MDAARIFNDDPWLYDNFHIVMDRIQPGVGINSNLGELKSYDVRNSIHSTYMRLKVRINATAPLKNGKCGLLGHTDKVCPDLFELEFNDGVRNWVPDLKPASSRIRSAANNKWLSDPIPSAVPRHHVPAGVSHVGRNSHTSGAANAVTFSDCMLAVDNQISALKHDVLAAQNIAKAKHGSGANFSCQMQFLPSSSTGTSASNLITGCPLVLGLPAGPFPNANSEESQDATGSELKKRKRMMALHNGETTSFAADGGEGNLVIGTNVSRGGMLI