AlfalfaGEDB | MS.gene80362

M. sativa cultivar XinJiangDaYe / MS.gene80362

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80362.t1	PNY16581.1	68.8	337	102	2	1	337	1	334	2.20E-128	468.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80362.t1	A0A2K3PMS8	68.8	337	102	2	1	337	1	334	1.6e-128	468.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61452	MS.gene80362	PPI
MS.gene83772	MS.gene80362	PPI
MS.gene80362	MS.gene29321	PPI
MS.gene008927	MS.gene80362	PPI
MS.gene016102	MS.gene80362	PPI
MS.gene46941	MS.gene80362	PPI
MS.gene045614	MS.gene80362	PPI
MS.gene96541	MS.gene80362	PPI
MS.gene000634	MS.gene80362	PPI
MS.gene80362	MS.gene037049	PPI
MS.gene63762	MS.gene80362	PPI
MS.gene80362	MS.gene24278	PPI
MS.gene30208	MS.gene80362	PPI
MS.gene80362	MS.gene34618	PPI
MS.gene025873	MS.gene80362	PPI
MS.gene012019	MS.gene80362	PPI
MS.gene80362	MS.gene59460	PPI
MS.gene80362	MS.gene060773	PPI
MS.gene80362	MS.gene66650	PPI
MS.gene013676	MS.gene80362	PPI
MS.gene05358	MS.gene80362	PPI
MS.gene80362	MS.gene08713	PPI
MS.gene95561	MS.gene80362	PPI
MS.gene065627	MS.gene80362	PPI
MS.gene80362	MS.gene24070	PPI
MS.gene018745	MS.gene80362	PPI
MS.gene000629	MS.gene80362	PPI
MS.gene65546	MS.gene80362	PPI
MS.gene80362	MS.gene85538	PPI
MS.gene80362	MS.gene26844	PPI
MS.gene80362	MS.gene64178	PPI
MS.gene03936	MS.gene80362	PPI
MS.gene05319	MS.gene80362	PPI
MS.gene80362	MS.gene074257	PPI
MS.gene038098	MS.gene80362	PPI
MS.gene56991	MS.gene80362	PPI
MS.gene80362	MS.gene073573	PPI
MS.gene60059	MS.gene80362	PPI
MS.gene80362	MS.gene44144	PPI
MS.gene80362	MS.gene05576	PPI
MS.gene80362	MS.gene060718	PPI
MS.gene28545	MS.gene80362	PPI
MS.gene28718	MS.gene80362	PPI
MS.gene80362	MS.gene48477	PPI
MS.gene80362	MS.gene027169	PPI
MS.gene80362	MS.gene004417	PPI
MS.gene038757	MS.gene80362	PPI
MS.gene064966	MS.gene80362	PPI
MS.gene80362	MS.gene019177	PPI
MS.gene80362	MS.gene055560	PPI
MS.gene80362	MS.gene009213	PPI
MS.gene80362	MS.gene98127	PPI
MS.gene80362	MS.gene003264	PPI
MS.gene80362	MS.gene054652	PPI
MS.gene43924	MS.gene80362	PPI
MS.gene046089	MS.gene80362	PPI
MS.gene036423	MS.gene80362	PPI
MS.gene80362	MS.gene27329	PPI
MS.gene80362	MS.gene41393	PPI
MS.gene72520	MS.gene80362	PPI
MS.gene013193	MS.gene80362	PPI
MS.gene80362	MS.gene89815	PPI
MS.gene66651	MS.gene80362	PPI
MS.gene80362	MS.gene016632	PPI
MS.gene80362	MS.gene018474	PPI
MS.gene80362	MS.gene048088	PPI
MS.gene80362	MS.gene86868	PPI
MS.gene35202	MS.gene80362	PPI
MS.gene051449	MS.gene80362	PPI
MS.gene63743	MS.gene80362	PPI
MS.gene80362	MS.gene058889	PPI
MS.gene80362	MS.gene96163	PPI
MS.gene48477	MS.gene80362	PPI
MS.gene30136	MS.gene80362	PPI
MS.gene008861	MS.gene80362	PPI
MS.gene024405	MS.gene80362	PPI
MS.gene80362	MS.gene34463	PPI
MS.gene012748	MS.gene80362	PPI
MS.gene83979	MS.gene80362	PPI
MS.gene85295	MS.gene80362	PPI
MS.gene80362	MS.gene026680	PPI
MS.gene80362	MS.gene61499	PPI
MS.gene80362	MS.gene51804	PPI
MS.gene47792	MS.gene80362	PPI
MS.gene46948	MS.gene80362	PPI
MS.gene98471	MS.gene80362	PPI
MS.gene80362	MS.gene019165	PPI
MS.gene80362	MS.gene81194	PPI
MS.gene93396	MS.gene80362	PPI
MS.gene80362	MS.gene038394	PPI
MS.gene05576	MS.gene80362	PPI
MS.gene80362	MS.gene019001	PPI
MS.gene05231	MS.gene80362	PPI
MS.gene80362	MS.gene53904	PPI
MS.gene80362	MS.gene73261	PPI
MS.gene010656	MS.gene80362	PPI
MS.gene80362	MS.gene025675	PPI
MS.gene041264	MS.gene80362	PPI
MS.gene41979	MS.gene80362	PPI
MS.gene80362	MS.gene055546	PPI
MS.gene80362	MS.gene71637	PPI
MS.gene64130	MS.gene80362	PPI
MS.gene80362	MS.gene55209	PPI
MS.gene050679	MS.gene80362	PPI
MS.gene80362	MS.gene28718	PPI
MS.gene20246	MS.gene80362	PPI
MS.gene055223	MS.gene80362	PPI
MS.gene80362	MS.gene022929	PPI
MS.gene071784	MS.gene80362	PPI
MS.gene053747	MS.gene80362	PPI
MS.gene76420	MS.gene80362	PPI
MS.gene80362	MS.gene068629	PPI
MS.gene65471	MS.gene80362	PPI
MS.gene061850	MS.gene80362	PPI
MS.gene80362	MS.gene009781	PPI
MS.gene80362	MS.gene021647	PPI
MS.gene26433	MS.gene80362	PPI
MS.gene80362	MS.gene048342	PPI
MS.gene80362	MS.gene069624	PPI
MS.gene042559	MS.gene80362	PPI
MS.gene80362	MS.gene21066	PPI
MS.gene80362	MS.gene013890	PPI
MS.gene80362	MS.gene073582	PPI
MS.gene98616	MS.gene80362	PPI
MS.gene80362	MS.gene016719	PPI
MS.gene80362	MS.gene028714	PPI
MS.gene042027	MS.gene80362	PPI
MS.gene85308	MS.gene80362	PPI
MS.gene80362	MS.gene048706	PPI
MS.gene69490	MS.gene80362	PPI
MS.gene80362	MS.gene69586	PPI
MS.gene80362	MS.gene45083	PPI
MS.gene80362	MS.gene049561	PPI
MS.gene015248	MS.gene80362	PPI
MS.gene80362	MS.gene008658	PPI
MS.gene80362	MS.gene49057	PPI
MS.gene44083	MS.gene80362	PPI
MS.gene80362	MS.gene03936	PPI
MS.gene04970	MS.gene80362	PPI
MS.gene08106	MS.gene80362	PPI
MS.gene58419	MS.gene80362	PPI
MS.gene067340	MS.gene80362	PPI
MS.gene80362	MS.gene08708	PPI
MS.gene80362	MS.gene86275	PPI
MS.gene80362	MS.gene034643	PPI
MS.gene23521	MS.gene80362	PPI
MS.gene29406	MS.gene80362	PPI
MS.gene87529	MS.gene80362	PPI
MS.gene80362	MS.gene012889	PPI
MS.gene04134	MS.gene80362	PPI
MS.gene80362	MS.gene038757	PPI
MS.gene80362	MS.gene000506	PPI
MS.gene062067	MS.gene80362	PPI
MS.gene80362	MS.gene028716	PPI
MS.gene57044	MS.gene80362	PPI
MS.gene80362	MS.gene74639	PPI
MS.gene80362	MS.gene071133	PPI
MS.gene80362	MS.gene60266	PPI
MS.gene80362	MS.gene98467	PPI
MS.gene80362	MS.gene013644	PPI
MS.gene80362	MS.gene024522	PPI
MS.gene39956	MS.gene80362	PPI
MS.gene80362	MS.gene54051	PPI
MS.gene80362	MS.gene22111	PPI
MS.gene038676	MS.gene80362	PPI
MS.gene80362	MS.gene049610	PPI
MS.gene71637	MS.gene80362	PPI
MS.gene80362	MS.gene37955	PPI
MS.gene042651	MS.gene80362	PPI
MS.gene80362	MS.gene045875	PPI
MS.gene80362	MS.gene010890	PPI
MS.gene80362	MS.gene84790	PPI
MS.gene80362	MS.gene061579	PPI
MS.gene034643	MS.gene80362	PPI
MS.gene80362	MS.gene04970	PPI
MS.gene80362	MS.gene060788	PPI
MS.gene80362	MS.gene43924	PPI
MS.gene71588	MS.gene80362	PPI
MS.gene80362	MS.gene41643	PPI
MS.gene80362	MS.gene77990	PPI
MS.gene04749	MS.gene80362	PPI
MS.gene80362	MS.gene062511	PPI
MS.gene80362	MS.gene027994	PPI
MS.gene80362	MS.gene26433	PPI
MS.gene80362	MS.gene007022	PPI
MS.gene061579	MS.gene80362	PPI
MS.gene80362	MS.gene69584	PPI
MS.gene80362	MS.gene52349	PPI
MS.gene071320	MS.gene80362	PPI
MS.gene42903	MS.gene80362	PPI
MS.gene81712	MS.gene80362	PPI
MS.gene80362	MS.gene76420	PPI
MS.gene80362	MS.gene46353	PPI
MS.gene026680	MS.gene80362	PPI
MS.gene021555	MS.gene80362	PPI
MS.gene048747	MS.gene80362	PPI
MS.gene80362	MS.gene61651	PPI
MS.gene22200	MS.gene80362	PPI
MS.gene80362	MS.gene29406	PPI
MS.gene26038	MS.gene80362	PPI
MS.gene80362	MS.gene88393	PPI
MS.gene80362	MS.gene054508	PPI
MS.gene94654	MS.gene80362	PPI
MS.gene80362	MS.gene95960	PPI
MS.gene80362	MS.gene053373	PPI
MS.gene031967	MS.gene80362	PPI
MS.gene80362	MS.gene041264	PPI
MS.gene34812	MS.gene80362	PPI
MS.gene02896	MS.gene80362	PPI
MS.gene80362	MS.gene63150	PPI
MS.gene063214	MS.gene80362	PPI
MS.gene97410	MS.gene80362	PPI
MS.gene32415	MS.gene80362	PPI
MS.gene035626	MS.gene80362	PPI
MS.gene80362	MS.gene072852	PPI
MS.gene80362	MS.gene65474	PPI
MS.gene80362	MS.gene025401	PPI
MS.gene80362	MS.gene015093	PPI
MS.gene65623	MS.gene80362	PPI
MS.gene80362	MS.gene06164	PPI
MS.gene80362	MS.gene50392	PPI
MS.gene80362	MS.gene22730	PPI
MS.gene71675	MS.gene80362	PPI
MS.gene80362	MS.gene000379	PPI
MS.gene80362	MS.gene032299	PPI
MS.gene80362	MS.gene017586	PPI
MS.gene69495	MS.gene80362	PPI
MS.gene049512	MS.gene80362	PPI
MS.gene038623	MS.gene80362	PPI
MS.gene80362	MS.gene037634	PPI
MS.gene80362	MS.gene057828	PPI
MS.gene80362	MS.gene08606	PPI
MS.gene05357	MS.gene80362	PPI
MS.gene032311	MS.gene80362	PPI
MS.gene80362	MS.gene000640	PPI
MS.gene030090	MS.gene80362	PPI
MS.gene96174	MS.gene80362	PPI
MS.gene80362	MS.gene09517	PPI
MS.gene80362	MS.gene98756	PPI
MS.gene018196	MS.gene80362	PPI
MS.gene80362	MS.gene87845	PPI
MS.gene86849	MS.gene80362	PPI
MS.gene57019	MS.gene80362	PPI
MS.gene80362	MS.gene62249	PPI
MS.gene80362	MS.gene58419	PPI
MS.gene021968	MS.gene80362	PPI
MS.gene80362	MS.gene010290	PPI
MS.gene09589	MS.gene80362	PPI
MS.gene80362	MS.gene92517	PPI
MS.gene80362	MS.gene36069	PPI
MS.gene43393	MS.gene80362	PPI
MS.gene80362	MS.gene044729	PPI
MS.gene34197	MS.gene80362	PPI
MS.gene80362	MS.gene60390	PPI
MS.gene008275	MS.gene80362	PPI
MS.gene80362	MS.gene039692	PPI
MS.gene80362	MS.gene55682	PPI
MS.gene80362	MS.gene002590	PPI
MS.gene005120	MS.gene80362	PPI
MS.gene048072	MS.gene80362	PPI
MS.gene060386	MS.gene80362	PPI
MS.gene05436	MS.gene80362	PPI
MS.gene80362	MS.gene024297	PPI
MS.gene80362	MS.gene059269	PPI
MS.gene059736	MS.gene80362	PPI
MS.gene80362	MS.gene057934	PPI
MS.gene70932	MS.gene80362	PPI
MS.gene047954	MS.gene80362	PPI
MS.gene35175	MS.gene80362	PPI
MS.gene80362	MS.gene37537	PPI
MS.gene80362	MS.gene024841	PPI
MS.gene80362	MS.gene069786	PPI
MS.gene80362	MS.gene42010	PPI
MS.gene80362	MS.gene025158	PPI
MS.gene80362	MS.gene40828	PPI
MS.gene021965	MS.gene80362	PPI
MS.gene055683	MS.gene80362	PPI
MS.gene042614	MS.gene80362	PPI
MS.gene80362	MS.gene51799	PPI
MS.gene80362	MS.gene98471	PPI
MS.gene96109	MS.gene80362	PPI
MS.gene80362	MS.gene40469	PPI
MS.gene80362	MS.gene09023	PPI
MS.gene009121	MS.gene80362	PPI
MS.gene80362	MS.gene025987	PPI
MS.gene80362	MS.gene043140	PPI
MS.gene36069	MS.gene80362	PPI
MS.gene80362	MS.gene03149	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80362.t1	MTR_0010s0370	31.250	144	98	1	35	177	34	177	1.28e-21	91.3
MS.gene80362.t1	MTR_0010s0350	31.250	144	98	1	35	177	34	177	1.28e-21	91.3
MS.gene80362.t1	MTR_0008s0210	35.862	145	51	3	47	184	4	113	1.87e-17	79.0
MS.gene80362.t1	MTR_6g045237	28.729	181	125	2	50	226	96	276	3.23e-16	79.7
MS.gene80362.t1	MTR_6g033215	24.855	173	124	3	29	197	123	293	6.87e-16	77.4
MS.gene80362.t1	MTR_0008s0180	38.739	111	33	2	74	184	19	94	1.27e-15	73.6
MS.gene80362.t1	MTR_7g106180	23.944	142	108	0	34	175	25	166	8.31e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80362.t1	AT5G36228	29.940	167	113	3	58	222	62	226	1.62e-16	80.1
MS.gene80362.t1	AT3G31430	31.098	164	105	5	74	234	173	331	9.17e-16	77.8
MS.gene80362.t1	AT2G01050	26.347	167	118	4	49	214	94	256	3.21e-14	73.9

Find 99 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGCTTCACAATCCTTAATT+TGG	0.175074	6.3:-27719943	None:intergenic
TGAAGGTTACAATGGATTTA+AGG	0.212543	6.3:+27719810	MS.gene80362:CDS
TTGTCAAGCTTTCCCATTAC+TGG	0.233212	6.3:-27720304	None:intergenic
GATGCAATGGCCTTGAAAAT+AGG	0.277457	6.3:+27719719	MS.gene80362:CDS
TCAAATCGGCCACCAAATTA+AGG	0.288401	6.3:+27719931	MS.gene80362:CDS
TGCAGCAAGACCCTCTTTCC+AGG	0.321400	6.3:+27720100	MS.gene80362:CDS
TTCAGGTCCTGTACTTCAAC+TGG	0.329203	6.3:-27719503	None:intergenic
ACCACAGTCCCTCGCTTTAG+AGG	0.331005	6.3:-27719836	None:intergenic
ATGCAATGGCCTTGAAAATA+GGG	0.350671	6.3:+27719720	MS.gene80362:CDS
TCAGCTTCCTGATTTGTAAC+CGG	0.361076	6.3:-27720220	None:intergenic
CTACACTTTCCACTGCTGTA+TGG	0.372549	6.3:-27720249	None:intergenic
GTTATTTCATCCCCAGTAAT+GGG	0.373895	6.3:+27720292	MS.gene80362:CDS
TAGATAACAAAGAGGGTAAC+AGG	0.379608	6.3:+27719768	MS.gene80362:CDS
AAAGAGAACAACTCTGGTTC+TGG	0.380312	6.3:+27720073	MS.gene80362:CDS
GTTCGTATTTGAAGAAGATT+CGG	0.385858	6.3:-27719880	None:intergenic
TGATTTGTAACCGGGACAAT+AGG	0.387747	6.3:-27720211	None:intergenic
TCTTTGTTGATGCTAGTTCC+TGG	0.395123	6.3:-27720118	None:intergenic
GTACTTCAACTGGATTCTTC+AGG	0.397650	6.3:-27719493	None:intergenic
TATTGTCCCGGTTACAAATC+AGG	0.398896	6.3:+27720213	MS.gene80362:CDS
TTGGAGCTTCGATCGGAATC+TGG	0.417176	6.3:+27719592	MS.gene80362:CDS
GGGGAAGTTCTTACAGTTGA+AGG	0.422619	6.3:+27719793	MS.gene80362:CDS
CTGAAGATTCTTTCAACTCT+AGG	0.423269	6.3:+27719438	MS.gene80362:CDS
TGTTATGGAGAGCTGGAAGA+AGG	0.426485	6.3:+27719313	None:intergenic
TGTCAAGCTTTCCCATTACT+GGG	0.428575	6.3:-27720303	None:intergenic
GGAAAGTGTAGCCGAGTCAT+TGG	0.442806	6.3:+27720261	MS.gene80362:CDS
CTGTCCTCAAGAACGGGCCT+TGG	0.445626	6.3:+27719573	MS.gene80362:CDS
CAGCTTCCTGATTTGTAACC+GGG	0.447262	6.3:-27720219	None:intergenic
ATCTGCTGAAGGCAGAAGTA+AGG	0.452812	6.3:+27720144	MS.gene80362:CDS
CTCTGAAGGTTGTTCATCTC+CGG	0.454764	6.3:-27719633	None:intergenic
CTTCAGCACGAAGAAGGATC+TGG	0.460669	6.3:+27719547	MS.gene80362:CDS
TACACTTTCCACTGCTGTAT+GGG	0.461621	6.3:-27720248	None:intergenic
TGGATACTGTCCTCAAGAAC+GGG	0.463935	6.3:+27719567	MS.gene80362:CDS
ATGAAGGAAGAGGAAGAAGA+TGG	0.465430	6.3:+27719344	MS.gene80362:CDS
CTTCACAATCCTTAATTTGG+TGG	0.466672	6.3:-27719940	None:intergenic
GGTTATTTCATCCCCAGTAA+TGG	0.473513	6.3:+27720291	MS.gene80362:CDS
TACAAATCAGGAAGCTGAAA+AGG	0.478682	6.3:+27720225	MS.gene80362:CDS
TGAAAATAGGGAATAGCCTT+GGG	0.483078	6.3:+27719732	MS.gene80362:CDS
GGAGTCTGAACTTGATGACT+TGG	0.495602	6.3:+27719979	MS.gene80362:CDS
AATTAAGGATTGTGAAGCTA+TGG	0.495659	6.3:+27719946	MS.gene80362:CDS
TGAAGCTATGGAAGGAAAAG+AGG	0.498103	6.3:+27719958	MS.gene80362:CDS
TTGAAAATAGGGAATAGCCT+TGG	0.498458	6.3:+27719731	MS.gene80362:CDS
GATGGGTTAGGCAGAAGGTG+GGG	0.499898	6.3:+27720371	MS.gene80362:intron
TTAAATTCACCAGAGAACTT+TGG	0.501243	6.3:-27720049	None:intergenic
GAAAGTGTAGCCGAGTCATT+GGG	0.502935	6.3:+27720262	MS.gene80362:CDS
GGTAGATCTAATGAAGGAAG+AGG	0.504019	6.3:+27719334	MS.gene80362:CDS
GTTTCGCTTCAGCACGAAGA+AGG	0.506692	6.3:+27719541	MS.gene80362:CDS
TGAAGGAAGAGGAAGAAGAT+GGG	0.507084	6.3:+27719345	MS.gene80362:CDS
TGATGCTAGTTCCTGGAAAG+AGG	0.512409	6.3:-27720111	None:intergenic
TCCCCACTACCAAAGTTCTC+TGG	0.513815	6.3:+27720040	MS.gene80362:CDS
AGCTCCAAGGCCCGTTCTTG+AGG	0.519814	6.3:-27719577	None:intergenic
AAGAACTACCATTTGGGAAG+TGG	0.520667	6.3:+27720008	MS.gene80362:CDS
GAAGAATCCAGTTGAAGTAC+AGG	0.524985	6.3:+27719496	MS.gene80362:CDS
AACAAAGAGGGTAACAGGAT+GGG	0.528525	6.3:+27719773	MS.gene80362:CDS
CACCAGAGAACTTTGGTAGT+GGG	0.531457	6.3:-27720042	None:intergenic
TAACAAAGAGGGTAACAGGA+TGG	0.532014	6.3:+27719772	MS.gene80362:CDS
GCTCTCAGCCACTTCCCAAA+TGG	0.532589	6.3:-27720016	None:intergenic
TCACCAGAGAACTTTGGTAG+TGG	0.536398	6.3:-27720043	None:intergenic
GTCATCCTCAAGAGAGTCAC+CGG	0.538632	6.3:+27719614	MS.gene80362:CDS
ACAAAGAGGGTAACAGGATG+GGG	0.544293	6.3:+27719774	MS.gene80362:CDS
AGATTCGGAGATCTTTACCT+TGG	0.544535	6.3:-27719865	None:intergenic
GGAAGAAGATGGGTTTGTTG+CGG	0.545891	6.3:+27719355	MS.gene80362:CDS
AAGGTGGGGAAGAAAGCGAC+AGG	0.548704	6.3:+27720385	MS.gene80362:CDS
CTTACAGTTGAAGGTTACAA+TGG	0.549944	6.3:+27719802	MS.gene80362:CDS
GAAAACAGCGGCATCTCTGA+AGG	0.554781	6.3:-27719647	None:intergenic
GCCTCTAAAGCGAGGGACTG+TGG	0.555109	6.3:+27719835	MS.gene80362:CDS
CTGGATACTGTCCTCAAGAA+CGG	0.559181	6.3:+27719566	MS.gene80362:CDS
CAGAAGTCAACCTATTGTCC+CGG	0.560005	6.3:+27720201	MS.gene80362:CDS
AGATGGGTTAGGCAGAAGGT+GGG	0.567713	6.3:+27720370	MS.gene80362:intron
GATCTCTGGTTGGTAAACTC+TGG	0.568705	6.3:+27719414	MS.gene80362:CDS
CAGATCTAAGCTTGAGAGGG+AGG	0.577519	6.3:-27719697	None:intergenic
GTGGAGGTAGATAACAAAGA+GGG	0.577520	6.3:+27719761	MS.gene80362:CDS
ACGGGCCTTGGAGCTTCGAT+CGG	0.578220	6.3:+27719585	MS.gene80362:CDS
CATCTCCGGTGACTCTCTTG+AGG	0.580032	6.3:-27719619	None:intergenic
AACAAAGAGGCATCTGCTGA+AGG	0.581354	6.3:+27720133	MS.gene80362:CDS
GTCAAGCTTTCCCATTACTG+GGG	0.582614	6.3:-27720302	None:intergenic
GCGGATGATGTTGAAGTATG+TGG	0.584059	6.3:+27719374	MS.gene80362:CDS
GGTTGAGCAGAATTCGCTAC+CGG	0.586105	6.3:+27720174	MS.gene80362:CDS
AGGCAGAAGTAAGGAAGTTG+AGG	0.587856	6.3:+27720153	MS.gene80362:CDS
GAAGAAGGTAGATCTAATGA+AGG	0.590584	6.3:+27719328	MS.gene80362:CDS
AAGGATTGTGAAGCTATGGA+AGG	0.594634	6.3:+27719950	MS.gene80362:CDS
ATAGGTTGACTTCTGAGAGC+CGG	0.595524	6.3:-27720193	None:intergenic
GATGCTAGTTCCTGGAAAGA+GGG	0.596123	6.3:-27720110	None:intergenic
CAAGCTTAGATCTGATGCAA+TGG	0.600092	6.3:+27719706	MS.gene80362:CDS
AGCCGAGTCATTGGGAAATG+TGG	0.607952	6.3:+27720270	MS.gene80362:CDS
ACCAGAGAACTTTGGTAGTG+GGG	0.615053	6.3:-27720041	None:intergenic
AACCACATTTCCCAATGACT+CGG	0.620224	6.3:-27720272	None:intergenic
AGATTCCGATCGAAGCTCCA+AGG	0.620359	6.3:-27719590	None:intergenic
TTAAGGAAGCCTCTAAAGCG+AGG	0.622687	6.3:+27719827	MS.gene80362:CDS
CATCAGATCTAAGCTTGAGA+GGG	0.626211	6.3:-27719700	None:intergenic
GCATCAGATCTAAGCTTGAG+AGG	0.633688	6.3:-27719701	None:intergenic
AGCAAGTTATCACTCAAGCA+TGG	0.635019	6.3:+27719468	MS.gene80362:CDS
TAAGGAAGCCTCTAAAGCGA+GGG	0.637552	6.3:+27719828	MS.gene80362:CDS
CTACCTCCACAAAAGTCCCA+AGG	0.645817	6.3:-27719748	None:intergenic
AGGAACTAGCATCAACAAAG+AGG	0.653024	6.3:+27720120	MS.gene80362:CDS
TGTGGAGGTAGATAACAAAG+AGG	0.660862	6.3:+27719760	MS.gene80362:CDS
GTAATGGGAAAGCTTGACAA+AGG	0.674297	6.3:+27720307	MS.gene80362:CDS
GGGACTGTGGTCAAATACCA+AGG	0.689047	6.3:+27719848	MS.gene80362:CDS
TGAAAAGGCCCATACAGCAG+TGG	0.692573	6.3:+27720240	MS.gene80362:CDS
CAGAAGTCCCCAGAAAACAG+CGG	0.694609	6.3:-27719659	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGAAAAAGAACTACCATTT+GGG	+	chr6.3:27720002-27720021	MS.gene80362:CDS	25.0%
!	TTTAAAAAAGAGAACAACTC+TGG	+	chr6.3:27720067-27720086	MS.gene80362:CDS	25.0%
	AATTAAGGATTGTGAAGCTA+TGG	+	chr6.3:27719946-27719965	MS.gene80362:CDS	30.0%
	GATGAAAAAGAACTACCATT+TGG	+	chr6.3:27720001-27720020	MS.gene80362:CDS	30.0%
	GTTCGTATTTGAAGAAGATT+CGG	-	chr6.3:27719883-27719902	None:intergenic	30.0%
	TAGCTTCACAATCCTTAATT+TGG	-	chr6.3:27719946-27719965	None:intergenic	30.0%
	TTAAATTCACCAGAGAACTT+TGG	-	chr6.3:27720052-27720071	None:intergenic	30.0%
!	ACACAAACAAGTTTTTGTTC+AGG	-	chr6.3:27719523-27719542	None:intergenic	30.0%
!	TGAAGGTTACAATGGATTTA+AGG	+	chr6.3:27719810-27719829	MS.gene80362:CDS	30.0%
!!!	AGAGGTTTTTGTTTTAGTTC+TGG	-	chr6.3:27720335-27720354	None:intergenic	30.0%
	ACAAAAACCTCTGATAAACC+TGG	+	chr6.3:27720343-27720362	MS.gene80362:intron	35.0%
	CTGAAGATTCTTTCAACTCT+AGG	+	chr6.3:27719438-27719457	MS.gene80362:CDS	35.0%
	CTTACAGTTGAAGGTTACAA+TGG	+	chr6.3:27719802-27719821	MS.gene80362:CDS	35.0%
	GAAGAAGGTAGATCTAATGA+AGG	+	chr6.3:27719328-27719347	MS.gene80362:CDS	35.0%
	TACAAATCAGGAAGCTGAAA+AGG	+	chr6.3:27720225-27720244	MS.gene80362:CDS	35.0%
	TAGATAACAAAGAGGGTAAC+AGG	+	chr6.3:27719768-27719787	MS.gene80362:CDS	35.0%
	TGAAAATAGGGAATAGCCTT+GGG	+	chr6.3:27719732-27719751	MS.gene80362:CDS	35.0%
	TTGAAAATAGGGAATAGCCT+TGG	+	chr6.3:27719731-27719750	MS.gene80362:CDS	35.0%
!	AAGGCTATTCCCTATTTTCA+AGG	-	chr6.3:27719732-27719751	None:intergenic	35.0%
!	ATGCAATGGCCTTGAAAATA+GGG	+	chr6.3:27719720-27719739	MS.gene80362:CDS	35.0%
!	CTTCACAATCCTTAATTTGG+TGG	-	chr6.3:27719943-27719962	None:intergenic	35.0%
!	GTTATTTCATCCCCAGTAAT+GGG	+	chr6.3:27720292-27720311	MS.gene80362:CDS	35.0%
!!	TGCTTTTTGTGTGGTCAAAT+CGG	+	chr6.3:27719917-27719936	MS.gene80362:CDS	35.0%
	AACAAAGAGGGTAACAGGAT+GGG	+	chr6.3:27719773-27719792	MS.gene80362:CDS	40.0%
	AACCACATTTCCCAATGACT+CGG	-	chr6.3:27720275-27720294	None:intergenic	40.0%
	AAGAACTACCATTTGGGAAG+TGG	+	chr6.3:27720008-27720027	MS.gene80362:CDS	40.0%
	AAGGATTGTGAAGCTATGGA+AGG	+	chr6.3:27719950-27719969	MS.gene80362:CDS	40.0%
	ACCACACAAAAAGCAGAAAG+TGG	-	chr6.3:27719912-27719931	None:intergenic	40.0%
	AGATTCGGAGATCTTTACCT+TGG	-	chr6.3:27719868-27719887	None:intergenic	40.0%
	AGCAAGTTATCACTCAAGCA+TGG	+	chr6.3:27719468-27719487	MS.gene80362:CDS	40.0%
	AGGAACTAGCATCAACAAAG+AGG	+	chr6.3:27720120-27720139	MS.gene80362:CDS	40.0%
	ATGAAGGAAGAGGAAGAAGA+TGG	+	chr6.3:27719344-27719363	MS.gene80362:CDS	40.0%
	CAAGCTTAGATCTGATGCAA+TGG	+	chr6.3:27719706-27719725	MS.gene80362:CDS	40.0%
	CATCAGATCTAAGCTTGAGA+GGG	-	chr6.3:27719703-27719722	None:intergenic	40.0%
	CCACACAAAAAGCAGAAAGT+GGG	-	chr6.3:27719911-27719930	None:intergenic	40.0%
	GAAGAATCCAGTTGAAGTAC+AGG	+	chr6.3:27719496-27719515	MS.gene80362:CDS	40.0%
	GGTAGATCTAATGAAGGAAG+AGG	+	chr6.3:27719334-27719353	MS.gene80362:CDS	40.0%
	GTACTTCAACTGGATTCTTC+AGG	-	chr6.3:27719496-27719515	None:intergenic	40.0%
	GTGGAGGTAGATAACAAAGA+GGG	+	chr6.3:27719761-27719780	MS.gene80362:CDS	40.0%
	TAACAAAGAGGGTAACAGGA+TGG	+	chr6.3:27719772-27719791	MS.gene80362:CDS	40.0%
	TACACTTTCCACTGCTGTAT+GGG	-	chr6.3:27720251-27720270	None:intergenic	40.0%
	TATTGTCCCGGTTACAAATC+AGG	+	chr6.3:27720213-27720232	MS.gene80362:CDS	40.0%
	TCAAATCGGCCACCAAATTA+AGG	+	chr6.3:27719931-27719950	MS.gene80362:CDS	40.0%
	TCTTTGTTGATGCTAGTTCC+TGG	-	chr6.3:27720121-27720140	None:intergenic	40.0%
	TGAAGCTATGGAAGGAAAAG+AGG	+	chr6.3:27719958-27719977	MS.gene80362:CDS	40.0%
	TGAAGGAAGAGGAAGAAGAT+GGG	+	chr6.3:27719345-27719364	MS.gene80362:CDS	40.0%
	TGATAAACCTGGCAAGAGAT+GGG	+	chr6.3:27720354-27720373	MS.gene80362:intron	40.0%
	TGTCAAGCTTTCCCATTACT+GGG	-	chr6.3:27720306-27720325	None:intergenic	40.0%
	TGTGGAGGTAGATAACAAAG+AGG	+	chr6.3:27719760-27719779	MS.gene80362:CDS	40.0%
	TTGTCAAGCTTTCCCATTAC+TGG	-	chr6.3:27720307-27720326	None:intergenic	40.0%
!	AAAGAGAACAACTCTGGTTC+TGG	+	chr6.3:27720073-27720092	MS.gene80362:CDS	40.0%
!	AGAGATTTTCACGAGATCTC+TGG	+	chr6.3:27719400-27719419	MS.gene80362:CDS	40.0%
!	ATTTTCACGAGATCTCTGGT+TGG	+	chr6.3:27719404-27719423	MS.gene80362:CDS	40.0%
!	GATGCAATGGCCTTGAAAAT+AGG	+	chr6.3:27719719-27719738	MS.gene80362:CDS	40.0%
!	GGTTATTTCATCCCCAGTAA+TGG	+	chr6.3:27720291-27720310	MS.gene80362:CDS	40.0%
!	GTAATGGGAAAGCTTGACAA+AGG	+	chr6.3:27720307-27720326	MS.gene80362:CDS	40.0%
!	TCAGCTTCCTGATTTGTAAC+CGG	-	chr6.3:27720223-27720242	None:intergenic	40.0%
!	TGATTTGTAACCGGGACAAT+AGG	-	chr6.3:27720214-27720233	None:intergenic	40.0%
	ACAAAGAGGGTAACAGGATG+GGG	+	chr6.3:27719774-27719793	MS.gene80362:CDS	45.0%
	AGGCAGAAGTAAGGAAGTTG+AGG	+	chr6.3:27720153-27720172	MS.gene80362:CDS	45.0%
	ATCTGCTGAAGGCAGAAGTA+AGG	+	chr6.3:27720144-27720163	MS.gene80362:CDS	45.0%
	CAGAAGTCAACCTATTGTCC+CGG	+	chr6.3:27720201-27720220	MS.gene80362:CDS	45.0%
	CTACACTTTCCACTGCTGTA+TGG	-	chr6.3:27720252-27720271	None:intergenic	45.0%
	CTGATAAACCTGGCAAGAGA+TGG	+	chr6.3:27720353-27720372	MS.gene80362:intron	45.0%
	CTGGATACTGTCCTCAAGAA+CGG	+	chr6.3:27719566-27719585	MS.gene80362:CDS	45.0%
	GATGCTAGTTCCTGGAAAGA+GGG	-	chr6.3:27720113-27720132	None:intergenic	45.0%
	GCATCAGATCTAAGCTTGAG+AGG	-	chr6.3:27719704-27719723	None:intergenic	45.0%
	GCGGATGATGTTGAAGTATG+TGG	+	chr6.3:27719374-27719393	MS.gene80362:CDS	45.0%
	GGGGAAGTTCTTACAGTTGA+AGG	+	chr6.3:27719793-27719812	MS.gene80362:CDS	45.0%
	GTCAAGCTTTCCCATTACTG+GGG	-	chr6.3:27720305-27720324	None:intergenic	45.0%
	TCACCAGAGAACTTTGGTAG+TGG	-	chr6.3:27720046-27720065	None:intergenic	45.0%
	TCTCTTGCCAGGTTTATCAG+AGG	-	chr6.3:27720353-27720372	None:intergenic	45.0%
	TGATGCTAGTTCCTGGAAAG+AGG	-	chr6.3:27720114-27720133	None:intergenic	45.0%
	TGGATACTGTCCTCAAGAAC+GGG	+	chr6.3:27719567-27719586	MS.gene80362:CDS	45.0%
	TTCAGGTCCTGTACTTCAAC+TGG	-	chr6.3:27719506-27719525	None:intergenic	45.0%
!	ATAGGTTGACTTCTGAGAGC+CGG	-	chr6.3:27720196-27720215	None:intergenic	45.0%
!	CACCAGAGAACTTTGGTAGT+GGG	-	chr6.3:27720045-27720064	None:intergenic	45.0%
!	CAGCTTCCTGATTTGTAACC+GGG	-	chr6.3:27720222-27720241	None:intergenic	45.0%
!	CTCTGAAGGTTGTTCATCTC+CGG	-	chr6.3:27719636-27719655	None:intergenic	45.0%
!	GAAAGTGTAGCCGAGTCATT+GGG	+	chr6.3:27720262-27720281	MS.gene80362:CDS	45.0%
!	GGAGTCTGAACTTGATGACT+TGG	+	chr6.3:27719979-27719998	MS.gene80362:CDS	45.0%
!!	AACAAAGAGGCATCTGCTGA+AGG	+	chr6.3:27720133-27720152	MS.gene80362:CDS	45.0%
!!	ACCAGAGAACTTTGGTAGTG+GGG	-	chr6.3:27720044-27720063	None:intergenic	45.0%
!!	GAATAGCCTTGGGACTTTTG+TGG	+	chr6.3:27719742-27719761	MS.gene80362:CDS	45.0%
!!	GATCTCTGGTTGGTAAACTC+TGG	+	chr6.3:27719414-27719433	MS.gene80362:CDS	45.0%
!!	GGAAGAAGATGGGTTTGTTG+CGG	+	chr6.3:27719355-27719374	MS.gene80362:CDS	45.0%
!!	TAAGGAAGCCTCTAAAGCGA+GGG	+	chr6.3:27719828-27719847	MS.gene80362:CDS	45.0%
!!	TTAAGGAAGCCTCTAAAGCG+AGG	+	chr6.3:27719827-27719846	MS.gene80362:CDS	45.0%
!!!	CCCACTTTCTGCTTTTTGTG+TGG	+	chr6.3:27719908-27719927	MS.gene80362:CDS	45.0%
	AGATTCCGATCGAAGCTCCA+AGG	-	chr6.3:27719593-27719612	None:intergenic	50.0%
	AGCCGAGTCATTGGGAAATG+TGG	+	chr6.3:27720270-27720289	MS.gene80362:CDS	50.0%
	CAGAAGTCCCCAGAAAACAG+CGG	-	chr6.3:27719662-27719681	None:intergenic	50.0%
	CAGATCTAAGCTTGAGAGGG+AGG	-	chr6.3:27719700-27719719	None:intergenic	50.0%
	CTACCTCCACAAAAGTCCCA+AGG	-	chr6.3:27719751-27719770	None:intergenic	50.0%
	CTTCAGCACGAAGAAGGATC+TGG	+	chr6.3:27719547-27719566	MS.gene80362:CDS	50.0%
	GAAAACAGCGGCATCTCTGA+AGG	-	chr6.3:27719650-27719669	None:intergenic	50.0%
	GGGACTGTGGTCAAATACCA+AGG	+	chr6.3:27719848-27719867	MS.gene80362:CDS	50.0%
	GGTTGAGCAGAATTCGCTAC+CGG	+	chr6.3:27720174-27720193	MS.gene80362:CDS	50.0%
	GTCATCCTCAAGAGAGTCAC+CGG	+	chr6.3:27719614-27719633	MS.gene80362:CDS	50.0%
	GTTTCGCTTCAGCACGAAGA+AGG	+	chr6.3:27719541-27719560	MS.gene80362:CDS	50.0%
	TCCCCACTACCAAAGTTCTC+TGG	+	chr6.3:27720040-27720059	MS.gene80362:CDS	50.0%
	TGAAAAGGCCCATACAGCAG+TGG	+	chr6.3:27720240-27720259	MS.gene80362:CDS	50.0%
	TTGGAGCTTCGATCGGAATC+TGG	+	chr6.3:27719592-27719611	MS.gene80362:CDS	50.0%
!	AACCTGGCAAGAGATGGGTT+AGG	+	chr6.3:27720359-27720378	MS.gene80362:intron	50.0%
!	GGAAAGTGTAGCCGAGTCAT+TGG	+	chr6.3:27720261-27720280	MS.gene80362:CDS	50.0%
!!	AGAGATGCCGCTGTTTTCTG+GGG	+	chr6.3:27719652-27719671	MS.gene80362:CDS	50.0%
!!	AGATGGGTTAGGCAGAAGGT+GGG	+	chr6.3:27720370-27720389	MS.gene80362:intron	50.0%
!!	CAAGAGATGGGTTAGGCAGA+AGG	+	chr6.3:27720366-27720385	MS.gene80362:intron	50.0%
!!	CAGAGATGCCGCTGTTTTCT+GGG	+	chr6.3:27719651-27719670	MS.gene80362:CDS	50.0%
!!	GCTGTTTTCTGGGGACTTCT+GGG	+	chr6.3:27719661-27719680	MS.gene80362:CDS	50.0%
!!	TAGCCTTGGGACTTTTGTGG+AGG	+	chr6.3:27719745-27719764	MS.gene80362:CDS	50.0%
!!	TCAGAGATGCCGCTGTTTTC+TGG	+	chr6.3:27719650-27719669	MS.gene80362:CDS	50.0%
	AAGGTGGGGAAGAAAGCGAC+AGG	+	chr6.3:27720385-27720404	MS.gene80362:CDS	55.0%
	ACCACAGTCCCTCGCTTTAG+AGG	-	chr6.3:27719839-27719858	None:intergenic	55.0%
	CATCTCCGGTGACTCTCTTG+AGG	-	chr6.3:27719622-27719641	None:intergenic	55.0%
	GCTCTCAGCCACTTCCCAAA+TGG	-	chr6.3:27720019-27720038	None:intergenic	55.0%
	TGCAGCAAGACCCTCTTTCC+AGG	+	chr6.3:27720100-27720119	MS.gene80362:CDS	55.0%
	TGCCTAACCCATCTCTTGCC+AGG	-	chr6.3:27720364-27720383	None:intergenic	55.0%
!!	CGCTGTTTTCTGGGGACTTC+TGG	+	chr6.3:27719660-27719679	MS.gene80362:CDS	55.0%
!!	GAGATGGGTTAGGCAGAAGG+TGG	+	chr6.3:27720369-27720388	MS.gene80362:intron	55.0%
!!	GATGGGTTAGGCAGAAGGTG+GGG	+	chr6.3:27720371-27720390	MS.gene80362:intron	55.0%
	ACGGGCCTTGGAGCTTCGAT+CGG	+	chr6.3:27719585-27719604	MS.gene80362:CDS	60.0%
	AGCTCCAAGGCCCGTTCTTG+AGG	-	chr6.3:27719580-27719599	None:intergenic	60.0%
	CTGTCCTCAAGAACGGGCCT+TGG	+	chr6.3:27719573-27719592	MS.gene80362:CDS	60.0%
!!	GCCTCTAAAGCGAGGGACTG+TGG	+	chr6.3:27719835-27719854	MS.gene80362:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	27719317	27720422	27719317	ID=MS.gene80362
chr6.3	mRNA	27719317	27720422	27719317	ID=MS.gene80362.t1;Parent=MS.gene80362
chr6.3	exon	27719317	27720328	27719317	ID=MS.gene80362.t1.exon1;Parent=MS.gene80362.t1
chr6.3	CDS	27719317	27720328	27719317	ID=cds.MS.gene80362.t1;Parent=MS.gene80362.t1
chr6.3	exon	27720385	27720422	27720385	ID=MS.gene80362.t1.exon2;Parent=MS.gene80362.t1
chr6.3	CDS	27720385	27720422	27720385	ID=cds.MS.gene80362.t1;Parent=MS.gene80362.t1

Gene Sequence

>MS.gene80362

ATGGAGAGCTGGAAGAAGGTAGATCTAATGAAGGAAGAGGAAGAAGATGGGTTTGTTGCGGATGATGTTGAAGTATGTGGAGAAGAGATTTTCACGAGATCTCTGGTTGGTAAACTCTGGACTGAAGATTCTTTCAACTCTAGGATCTTCAAGCAAGTTATCACTCAAGCATGGCGCCTGAAGAATCCAGTTGAAGTACAGGACCTGAACAAAAACTTGTTTGTGTTTCGCTTCAGCACGAAGAAGGATCTGGATACTGTCCTCAAGAACGGGCCTTGGAGCTTCGATCGGAATCTGGTCATCCTCAAGAGAGTCACCGGAGATGAACAACCTTCAGAGATGCCGCTGTTTTCTGGGGACTTCTGGGCGAGAGTGTACGACCTCCCTCTCAAGCTTAGATCTGATGCAATGGCCTTGAAAATAGGGAATAGCCTTGGGACTTTTGTGGAGGTAGATAACAAAGAGGGTAACAGGATGGGGAAGTTCTTACAGTTGAAGGTTACAATGGATTTAAGGAAGCCTCTAAAGCGAGGGACTGTGGTCAAATACCAAGGTAAAGATCTCCGAATCTTCTTCAAATACGAACGTTTACCCACTTTCTGCTTTTTGTGTGGTCAAATCGGCCACCAAATTAAGGATTGTGAAGCTATGGAAGGAAAAGAGGAGTCTGAACTTGATGACTTGGATGAAAAAGAACTACCATTTGGGAAGTGGCTGAGAGCGTCCCCACTACCAAAGTTCTCTGGTGAATTTAAAAAAGAGAACAACTCTGGTTCTGGTTCTTGCAGCAAGACCCTCTTTCCAGGAACTAGCATCAACAAAGAGGCATCTGCTGAAGGCAGAAGTAAGGAAGTTGAGGTTGAGCAGAATTCGCTACCGGCTCTCAGAAGTCAACCTATTGTCCCGGTTACAAATCAGGAAGCTGAAAAGGCCCATACAGCAGTGGAAAGTGTAGCCGAGTCATTGGGAAATGTGGTTATTTCATCCCCAGTAATGGGAAAGCTTGACAAAGGTTCCAGAACTAAAACAAAAACCTCTGATAAACCTGGCAAGAGATGGGTTAGGCAGAAGGTGGGGAAGAAAGCGACAGGAAAAACACAACTTAA

Protein sequence

>MS.gene80362.t1

MESWKKVDLMKEEEEDGFVADDVEVCGEEIFTRSLVGKLWTEDSFNSRIFKQVITQAWRLKNPVEVQDLNKNLFVFRFSTKKDLDTVLKNGPWSFDRNLVILKRVTGDEQPSEMPLFSGDFWARVYDLPLKLRSDAMALKIGNSLGTFVEVDNKEGNRMGKFLQLKVTMDLRKPLKRGTVVKYQGKDLRIFFKYERLPTFCFLCGQIGHQIKDCEAMEGKEESELDDLDEKELPFGKWLRASPLPKFSGEFKKENNSGSGSCSKTLFPGTSINKEASAEGRSKEVEVEQNSLPALRSQPIVPVTNQEAEKAHTAVESVAESLGNVVISSPVMGKLDKEGGEESDRKNTT