AlfalfaGEDB | MS.gene58419

M. sativa cultivar XinJiangDaYe / MS.gene58419

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58419.t1	AES70414.1	53.2	346	93	4	1	343	1	280	6.70E-90	341.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58419.t1	O04244	25.7	136	97	2	83	216	6	139	2.9e-09	64.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58419.t1	G7J017	53.2	346	93	4	1	343	1	280	4.8e-90	341.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene58419	MS.gene95960	PPI
MS.gene58419	MS.gene053373	PPI
MS.gene58419	MS.gene043140	PPI
MS.gene071784	MS.gene58419	PPI
MS.gene053747	MS.gene58419	PPI
MS.gene58419	MS.gene041264	PPI
MS.gene061579	MS.gene58419	PPI
MS.gene76420	MS.gene58419	PPI
MS.gene071320	MS.gene58419	PPI
MS.gene65471	MS.gene58419	PPI
MS.gene061850	MS.gene58419	PPI
MS.gene58419	MS.gene29321	PPI
MS.gene26433	MS.gene58419	PPI
MS.gene58419	MS.gene65474	PPI
MS.gene58419	MS.gene025401	PPI
MS.gene58419	MS.gene015093	PPI
MS.gene042559	MS.gene58419	PPI
MS.gene58419	MS.gene24278	PPI
MS.gene58419	MS.gene000379	PPI
MS.gene58419	MS.gene032299	PPI
MS.gene98616	MS.gene58419	PPI
MS.gene042027	MS.gene58419	PPI
MS.gene85308	MS.gene58419	PPI
MS.gene69490	MS.gene58419	PPI
MS.gene58419	MS.gene08713	PPI
MS.gene015248	MS.gene58419	PPI
MS.gene58419	MS.gene09517	PPI
MS.gene58419	MS.gene87845	PPI
MS.gene34812	MS.gene58419	PPI
MS.gene44083	MS.gene58419	PPI
MS.gene04970	MS.gene58419	PPI
MS.gene08106	MS.gene58419	PPI
MS.gene58419	MS.gene62249	PPI
MS.gene067340	MS.gene58419	PPI
MS.gene23521	MS.gene58419	PPI
MS.gene58419	MS.gene05576	PPI
MS.gene29406	MS.gene58419	PPI
MS.gene58419	MS.gene044729	PPI
MS.gene05357	MS.gene58419	PPI
MS.gene58419	MS.gene60390	PPI
MS.gene58419	MS.gene48477	PPI
MS.gene87529	MS.gene58419	PPI
MS.gene04134	MS.gene58419	PPI
MS.gene58419	MS.gene027169	PPI
MS.gene58419	MS.gene55682	PPI
MS.gene062067	MS.gene58419	PPI
MS.gene58419	MS.gene019177	PPI
MS.gene57044	MS.gene58419	PPI
MS.gene39956	MS.gene58419	PPI
MS.gene58419	MS.gene055560	PPI
MS.gene58419	MS.gene024297	PPI
MS.gene71637	MS.gene58419	PPI
MS.gene58419	MS.gene057934	PPI
MS.gene042651	MS.gene58419	PPI
MS.gene09589	MS.gene58419	PPI
MS.gene58419	MS.gene37537	PPI
MS.gene58419	MS.gene024841	PPI
MS.gene58419	MS.gene069786	PPI
MS.gene58419	MS.gene42010	PPI
MS.gene58419	MS.gene025158	PPI
MS.gene58419	MS.gene40828	PPI
MS.gene58419	MS.gene27329	PPI
MS.gene58419	MS.gene41393	PPI
MS.gene034643	MS.gene58419	PPI
MS.gene58419	MS.gene016632	PPI
MS.gene71588	MS.gene58419	PPI
MS.gene58419	MS.gene40469	PPI
MS.gene04749	MS.gene58419	PPI
MS.gene58419	MS.gene03149	PPI
MS.gene42903	MS.gene58419	PPI
MS.gene81712	MS.gene58419	PPI
MS.gene026680	MS.gene58419	PPI
MS.gene021555	MS.gene58419	PPI
MS.gene58419	MS.gene037049	PPI
MS.gene048747	MS.gene58419	PPI
MS.gene58419	MS.gene026680	PPI
MS.gene22200	MS.gene58419	PPI
MS.gene58419	MS.gene34618	PPI
MS.gene58419	MS.gene59460	PPI
MS.gene58419	MS.gene019165	PPI
MS.gene58419	MS.gene81194	PPI
MS.gene58419	MS.gene060773	PPI
MS.gene26038	MS.gene58419	PPI
MS.gene58419	MS.gene66650	PPI
MS.gene94654	MS.gene58419	PPI
MS.gene58419	MS.gene038394	PPI
MS.gene031967	MS.gene58419	PPI
MS.gene58419	MS.gene019001	PPI
MS.gene02896	MS.gene58419	PPI
MS.gene58419	MS.gene53904	PPI
MS.gene58419	MS.gene24070	PPI
MS.gene063214	MS.gene58419	PPI
MS.gene58419	MS.gene73261	PPI
MS.gene97410	MS.gene58419	PPI
MS.gene83772	MS.gene58419	PPI
MS.gene32415	MS.gene58419	PPI
MS.gene58419	MS.gene85538	PPI
MS.gene035626	MS.gene58419	PPI
MS.gene58419	MS.gene26844	PPI
MS.gene58419	MS.gene64178	PPI
MS.gene58419	MS.gene055546	PPI
MS.gene58419	MS.gene55209	PPI
MS.gene58419	MS.gene074257	PPI
MS.gene63762	MS.gene58419	PPI
MS.gene65623	MS.gene58419	PPI
MS.gene58419	MS.gene073573	PPI
MS.gene58419	MS.gene28718	PPI
MS.gene71675	MS.gene58419	PPI
MS.gene58419	MS.gene44144	PPI
MS.gene69495	MS.gene58419	PPI
MS.gene049512	MS.gene58419	PPI
MS.gene58419	MS.gene060718	PPI
MS.gene038623	MS.gene58419	PPI
MS.gene032311	MS.gene58419	PPI
MS.gene030090	MS.gene58419	PPI
MS.gene58419	MS.gene068629	PPI
MS.gene96174	MS.gene58419	PPI
MS.gene58419	MS.gene004417	PPI
MS.gene018196	MS.gene58419	PPI
MS.gene58419	MS.gene009781	PPI
MS.gene86849	MS.gene58419	PPI
MS.gene57019	MS.gene58419	PPI
MS.gene58419	MS.gene21066	PPI
MS.gene58419	MS.gene009213	PPI
MS.gene58419	MS.gene013890	PPI
MS.gene58419	MS.gene98127	PPI
MS.gene03936	MS.gene58419	PPI
MS.gene021968	MS.gene58419	PPI
MS.gene58419	MS.gene003264	PPI
MS.gene58419	MS.gene054652	PPI
MS.gene43393	MS.gene58419	PPI
MS.gene34197	MS.gene58419	PPI
MS.gene008275	MS.gene58419	PPI
MS.gene58419	MS.gene80362	PPI
MS.gene58419	MS.gene49057	PPI
MS.gene58419	MS.gene89815	PPI
MS.gene038757	MS.gene58419	PPI
MS.gene005120	MS.gene58419	PPI
MS.gene58419	MS.gene018474	PPI
MS.gene58419	MS.gene048088	PPI
MS.gene58419	MS.gene86868	PPI
MS.gene048072	MS.gene58419	PPI
MS.gene060386	MS.gene58419	PPI
MS.gene58419	MS.gene058889	PPI
MS.gene58419	MS.gene96163	PPI
MS.gene58419	MS.gene034643	PPI
MS.gene05436	MS.gene58419	PPI
MS.gene059736	MS.gene58419	PPI
MS.gene70932	MS.gene58419	PPI
MS.gene047954	MS.gene58419	PPI
MS.gene35175	MS.gene58419	PPI
MS.gene58419	MS.gene012889	PPI
MS.gene58419	MS.gene34463	PPI
MS.gene58419	MS.gene038757	PPI
MS.gene021965	MS.gene58419	PPI
MS.gene055683	MS.gene58419	PPI
MS.gene042614	MS.gene58419	PPI
MS.gene66651	MS.gene58419	PPI
MS.gene58419	MS.gene61499	PPI
MS.gene96109	MS.gene58419	PPI
MS.gene58419	MS.gene51804	PPI
MS.gene58419	MS.gene013644	PPI
MS.gene051449	MS.gene58419	PPI
MS.gene009121	MS.gene58419	PPI
MS.gene36069	MS.gene58419	PPI
MS.gene30136	MS.gene58419	PPI
MS.gene61452	MS.gene58419	PPI
MS.gene58419	MS.gene025675	PPI
MS.gene008927	MS.gene58419	PPI
MS.gene58419	MS.gene04970	PPI
MS.gene016102	MS.gene58419	PPI
MS.gene46941	MS.gene58419	PPI
MS.gene045614	MS.gene58419	PPI
MS.gene96541	MS.gene58419	PPI
MS.gene000634	MS.gene58419	PPI
MS.gene58419	MS.gene71637	PPI
MS.gene58419	MS.gene43924	PPI
MS.gene30208	MS.gene58419	PPI
MS.gene025873	MS.gene58419	PPI
MS.gene012019	MS.gene58419	PPI
MS.gene58419	MS.gene022929	PPI
MS.gene013676	MS.gene58419	PPI
MS.gene58419	MS.gene26433	PPI
MS.gene58419	MS.gene007022	PPI
MS.gene05358	MS.gene58419	PPI
MS.gene95561	MS.gene58419	PPI
MS.gene58419	MS.gene69584	PPI
MS.gene58419	MS.gene52349	PPI
MS.gene58419	MS.gene021647	PPI
MS.gene065627	MS.gene58419	PPI
MS.gene58419	MS.gene048342	PPI
MS.gene018745	MS.gene58419	PPI
MS.gene000629	MS.gene58419	PPI
MS.gene65546	MS.gene58419	PPI
MS.gene58419	MS.gene069624	PPI
MS.gene58419	MS.gene073582	PPI
MS.gene05319	MS.gene58419	PPI
MS.gene038098	MS.gene58419	PPI
MS.gene56991	MS.gene58419	PPI
MS.gene60059	MS.gene58419	PPI
MS.gene58419	MS.gene016719	PPI
MS.gene58419	MS.gene028714	PPI
MS.gene58419	MS.gene88393	PPI
MS.gene58419	MS.gene048706	PPI
MS.gene58419	MS.gene69586	PPI
MS.gene28545	MS.gene58419	PPI
MS.gene58419	MS.gene45083	PPI
MS.gene58419	MS.gene049561	PPI
MS.gene28718	MS.gene58419	PPI
MS.gene58419	MS.gene008658	PPI
MS.gene58419	MS.gene63150	PPI
MS.gene58419	MS.gene03936	PPI
MS.gene064966	MS.gene58419	PPI
MS.gene58419	MS.gene072852	PPI
MS.gene58419	MS.gene08708	PPI
MS.gene58419	MS.gene06164	PPI
MS.gene58419	MS.gene50392	PPI
MS.gene58419	MS.gene86275	PPI
MS.gene58419	MS.gene22730	PPI
MS.gene58419	MS.gene017586	PPI
MS.gene43924	MS.gene58419	PPI
MS.gene046089	MS.gene58419	PPI
MS.gene58419	MS.gene037634	PPI
MS.gene58419	MS.gene057828	PPI
MS.gene036423	MS.gene58419	PPI
MS.gene58419	MS.gene08606	PPI
MS.gene58419	MS.gene000640	PPI
MS.gene58419	MS.gene000506	PPI
MS.gene58419	MS.gene98756	PPI
MS.gene80362	MS.gene58419	PPI
MS.gene72520	MS.gene58419	PPI
MS.gene013193	MS.gene58419	PPI
MS.gene58419	MS.gene028716	PPI
MS.gene58419	MS.gene74639	PPI
MS.gene58419	MS.gene071133	PPI
MS.gene58419	MS.gene60266	PPI
MS.gene58419	MS.gene98467	PPI
MS.gene35202	MS.gene58419	PPI
MS.gene58419	MS.gene024522	PPI
MS.gene58419	MS.gene54051	PPI
MS.gene58419	MS.gene22111	PPI
MS.gene63743	MS.gene58419	PPI
MS.gene58419	MS.gene049610	PPI
MS.gene58419	MS.gene010290	PPI
MS.gene58419	MS.gene37955	PPI
MS.gene58419	MS.gene92517	PPI
MS.gene48477	MS.gene58419	PPI
MS.gene58419	MS.gene36069	PPI
MS.gene58419	MS.gene045875	PPI
MS.gene58419	MS.gene010890	PPI
MS.gene58419	MS.gene84790	PPI
MS.gene58419	MS.gene061579	PPI
MS.gene008861	MS.gene58419	PPI
MS.gene024405	MS.gene58419	PPI
MS.gene58419	MS.gene039692	PPI
MS.gene012748	MS.gene58419	PPI
MS.gene58419	MS.gene002590	PPI
MS.gene83979	MS.gene58419	PPI
MS.gene85295	MS.gene58419	PPI
MS.gene58419	MS.gene060788	PPI
MS.gene58419	MS.gene41643	PPI
MS.gene58419	MS.gene77990	PPI
MS.gene47792	MS.gene58419	PPI
MS.gene46948	MS.gene58419	PPI
MS.gene58419	MS.gene062511	PPI
MS.gene038676	MS.gene58419	PPI
MS.gene58419	MS.gene027994	PPI
MS.gene58419	MS.gene059269	PPI
MS.gene93396	MS.gene58419	PPI
MS.gene98471	MS.gene58419	PPI
MS.gene05576	MS.gene58419	PPI
MS.gene05231	MS.gene58419	PPI
MS.gene58419	MS.gene76420	PPI
MS.gene58419	MS.gene46353	PPI
MS.gene010656	MS.gene58419	PPI
MS.gene58419	MS.gene51799	PPI
MS.gene58419	MS.gene98471	PPI
MS.gene041264	MS.gene58419	PPI
MS.gene41979	MS.gene58419	PPI
MS.gene58419	MS.gene61651	PPI
MS.gene58419	MS.gene29406	PPI
MS.gene64130	MS.gene58419	PPI
MS.gene050679	MS.gene58419	PPI
MS.gene58419	MS.gene09023	PPI
MS.gene20246	MS.gene58419	PPI
MS.gene055223	MS.gene58419	PPI
MS.gene58419	MS.gene054508	PPI
MS.gene58419	MS.gene025987	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58419.t1	MTR_3g053920	53.179	346	93	4	1	343	1	280	1.13e-113	335
MS.gene58419.t1	MTR_2g061500	77.519	129	29	0	1	129	1	129	1.99e-70	219
MS.gene58419.t1	MTR_7g011710	88.235	85	10	0	55	139	1	85	8.38e-49	164
MS.gene58419.t1	MTR_4g103530	71.287	101	28	1	55	155	1	100	1.16e-45	157
MS.gene58419.t1	MTR_6g033215	35.260	173	110	1	29	199	122	294	1.39e-35	133
MS.gene58419.t1	MTR_0010s0370	34.302	172	107	3	11	177	7	177	1.63e-28	111
MS.gene58419.t1	MTR_0010s0350	34.302	172	107	3	11	177	7	177	1.63e-28	111
MS.gene58419.t1	MTR_8g463110	66.990	103	31	1	289	388	1	103	2.93e-27	115
MS.gene58419.t1	MTR_1g036720	31.731	104	69	1	11	112	7	110	2.90e-13	66.2
MS.gene58419.t1	MTR_7g106180	25.333	150	104	3	32	175	19	166	7.27e-13	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58419.t1	AT2G17920	26.761	213	150	3	8	216	9	219	8.87e-19	87.0
MS.gene58419.t1	AT5G36228	27.536	207	142	3	36	240	39	239	1.25e-18	87.4
MS.gene58419.t1	AT2G41590	26.829	164	116	2	55	216	58	219	1.16e-16	81.6
MS.gene58419.t1	AT3G57587	28.188	149	101	3	71	216	74	219	6.62e-16	79.3
MS.gene58419.t1	AT5G25200	26.512	215	148	4	8	216	9	219	1.14e-15	78.6
MS.gene58419.t1	AT5G18636	26.512	215	148	4	8	216	9	219	1.29e-15	78.6
MS.gene58419.t1	AT2G02103	26.829	164	116	2	55	216	58	219	1.68e-15	78.2
MS.gene58419.t1	AT2G13450	25.824	182	131	2	36	215	39	218	1.14e-14	75.9
MS.gene58419.t1	AT3G31430	25.824	182	129	3	36	215	134	311	1.29e-14	75.1
MS.gene58419.t1	AT3G47920	25.676	148	106	2	71	216	3	148	4.84e-13	70.1

Find 135 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACTCCTCAGAGTTGGTAAT+TGG	0.242886	8.3:+15770229	None:intergenic
GTTCCGCTGCTAATAATAAA+TGG	0.253881	8.3:-15770558	MS.gene58419:CDS
GTTTCAATTTCACCACAAAA+TGG	0.274429	8.3:-15771055	MS.gene58419:CDS
CAGACTCGGTGGCTAGGTTA+AGG	0.302707	8.3:+15771226	None:intergenic
GTGTATCTAAACATCGCTTT+AGG	0.328089	8.3:-15769275	MS.gene58419:intron
CTTAGCTGACCGTGAGATTC+AGG	0.332229	8.3:-15771163	MS.gene58419:CDS
TTGTTACCTTCTTCTTCTAT+AGG	0.332854	8.3:-15770316	MS.gene58419:CDS
CAAGGCATAGGCAGCTTTCT+AGG	0.333453	8.3:-15770865	MS.gene58419:CDS
CTGATGATGGTGTCCGCAAT+TGG	0.338507	8.3:-15770615	MS.gene58419:CDS
GCAGGATTAGGCTTCAGGTC+AGG	0.339579	8.3:+15770589	None:intergenic
GCTGGCCGCCCTTTGGTGCT+TGG	0.347355	8.3:-15770271	MS.gene58419:CDS
GGGGCAACATTAACATAAAT+AGG	0.349901	8.3:+15769312	None:intergenic
CTCGGCACAGCAGTAGGAAT+TGG	0.352359	8.3:+15770526	None:intergenic
TTCTGTCGACTTGAACCATT+TGG	0.355812	8.3:-15770944	MS.gene58419:CDS
AGCCATACGCTCACTGAAAT+AGG	0.356045	8.3:+15771137	None:intergenic
TACGGGCAGGATTAGGCTTC+AGG	0.356208	8.3:+15770584	None:intergenic
CCTATGCCTTGACCAACATT+TGG	0.367628	8.3:+15770877	None:intergenic
CTTACTTGCTGGCCGCCCTT+TGG	0.370582	8.3:-15770278	MS.gene58419:CDS
ACTTGAACCATTTGGACATA+TGG	0.371083	8.3:-15770936	MS.gene58419:CDS
ATCCTTGATCACTGTTTGAT+TGG	0.378099	8.3:-15771192	MS.gene58419:CDS
ACGATCGAAACACGCTTGCC+TGG	0.389839	8.3:+15771102	None:intergenic
CAGGTTCACGGTCTTCCCTT+TGG	0.395747	8.3:-15770910	MS.gene58419:CDS
GAACCAATACGGGCAGGATT+AGG	0.402221	8.3:+15770577	None:intergenic
TCAGCTTCAAACTTACTTGC+TGG	0.405901	8.3:-15770289	MS.gene58419:CDS
AGACTCGGTGGCTAGGTTAA+GGG	0.413831	8.3:+15771227	None:intergenic
CAAAGGGAAGACCGTGAACC+TGG	0.415175	8.3:+15770911	None:intergenic
ATGGCTCGTGCTTGGAAGCC+AGG	0.426942	8.3:-15771120	MS.gene58419:CDS
TGATGATGGTGTCCGCAATT+GGG	0.436404	8.3:-15770614	MS.gene58419:CDS
GGATACGGTCCATTACGATA+TGG	0.437208	8.3:+15770986	None:intergenic
GACATATGGGTCCAGGTTCA+CGG	0.437811	8.3:-15770922	MS.gene58419:CDS
TGTTTCAGGTGTGGTTTGCT+TGG	0.440387	8.3:-15770679	MS.gene58419:CDS
CACCACTAGGAGGAGAGTTT+CGG	0.446642	8.3:+15770470	None:intergenic
AATAGTGTTACGGACATCAT+AGG	0.456763	8.3:+15770831	None:intergenic
AAGCCTAATCCTGCCCGTAT+TGG	0.462034	8.3:-15770580	MS.gene58419:CDS
ATGAAATTTCACCATTCTGA+AGG	0.462585	8.3:+15770158	None:intergenic
CAGGATTAGGCTTCAGGTCA+GGG	0.464318	8.3:+15770590	None:intergenic
ATTTCATCATTTGCTGCTGA+TGG	0.466311	8.3:-15770142	MS.gene58419:CDS
TATTAGCAGCGGAACCAATA+CGG	0.472774	8.3:+15770566	None:intergenic
TCGGCACAGCAGTAGGAATT+GGG	0.473536	8.3:+15770527	None:intergenic
AACAAGAAGGTCATGTTTGA+GGG	0.473613	8.3:+15770390	None:intergenic
TTGTCCGGAGTTGTTCGAGC+TGG	0.474275	8.3:-15770641	MS.gene58419:CDS
ACGGACTTTCAGACGCATAT+AGG	0.475542	8.3:+15770801	None:intergenic
TGGCCTCACCCTTGCTGAAG+AGG	0.475784	8.3:-15771256	MS.gene58419:CDS
TTTCATCATTTGCTGCTGAT+GGG	0.478737	8.3:-15770141	MS.gene58419:CDS
GGCCTCACCCTTGCTGAAGA+GGG	0.479040	8.3:-15771255	MS.gene58419:CDS
GCGCAGACGACATGTCAGTT+TGG	0.481046	8.3:-15769152	MS.gene58419:intron
CAACAAGAAGGTCATGTTTG+AGG	0.483349	8.3:+15770389	None:intergenic
CAAACAGTGATCAAGGATAG+TGG	0.483405	8.3:+15771197	None:intergenic
CCTGCTGGTGTCTTTCATGC+TGG	0.485754	8.3:-15770493	MS.gene58419:CDS
GAGGAGTCTCAAGATGCTAC+TGG	0.487924	8.3:-15770214	MS.gene58419:CDS
GTAATTGGAAACGGTTCAGC+AGG	0.488936	8.3:+15770244	None:intergenic
CCTGGACCCATATGTCCAAA+TGG	0.490389	8.3:+15770929	None:intergenic
ACACACTGACAAAGTTTGTC+CGG	0.500003	8.3:-15770656	MS.gene58419:CDS
GATACACAAGGCCGTGGTGT+GGG	0.503369	8.3:+15769291	None:intergenic
CCATTTGGACATATGGGTCC+AGG	0.504581	8.3:-15770929	MS.gene58419:CDS
CTTGAACCATTTGGACATAT+GGG	0.505866	8.3:-15770935	MS.gene58419:CDS
CCGAGCCACTATGTTCCTGC+TGG	0.508741	8.3:-15770508	MS.gene58419:CDS
CATAAGCACCATTACTAACT+CGG	0.510210	8.3:+15770740	None:intergenic
CGATAATTTCCATATCGTAA+TGG	0.512245	8.3:-15770995	MS.gene58419:CDS
GTGAGCGTATGGCTCGTGCT+TGG	0.515599	8.3:-15771128	MS.gene58419:CDS
ATTAGCAGCGGAACCAATAC+GGG	0.517620	8.3:+15770567	None:intergenic
GGGTGAGGCCAGCGATATTG+AGG	0.519134	8.3:+15771268	None:intergenic
TCAGAGTTGGTAATTGGAAA+CGG	0.522460	8.3:+15770235	None:intergenic
TGCCTATTTCAGTGAGCGTA+TGG	0.524721	8.3:-15771139	MS.gene58419:CDS
ATTGAGGTGGGAAGGGTCCA+TGG	0.525018	8.3:+15771284	None:intergenic
TTCATCATTTGCTGCTGATG+GGG	0.534420	8.3:-15770140	MS.gene58419:CDS
CTGAACTGAAGAAGAGAAAG+AGG	0.535515	8.3:-15770189	MS.gene58419:CDS
ATACACAAGGCCGTGGTGTG+GGG	0.538349	8.3:+15769292	None:intergenic
GGTGAAATTGAAACAGAAAA+CGG	0.538716	8.3:+15771064	None:intergenic
AACCCTCTTCAGCAAGGGTG+AGG	0.538870	8.3:+15771253	None:intergenic
AGGTACTATGAATAGTGTTA+CGG	0.539531	8.3:+15770821	None:intergenic
ACAGAAGTGGGGACAACACC+AGG	0.541151	8.3:+15770961	None:intergenic
TTCGAGCTGGAGTCTGATGA+TGG	0.541255	8.3:-15770628	MS.gene58419:CDS
TAGTGGCTCGGCACAGCAGT+AGG	0.541285	8.3:+15770520	None:intergenic
GGCCAATCAAACAGTGATCA+AGG	0.541425	8.3:+15771190	None:intergenic
TGGTTCAAGTCGACAGAAGT+GGG	0.543153	8.3:+15770949	None:intergenic
GGACAACACCAGGCTGGATA+CGG	0.543289	8.3:+15770971	None:intergenic
CCAAATGTTGGTCAAGGCAT+AGG	0.543940	8.3:-15770877	MS.gene58419:CDS
GGTTGCATTCAACCAAATGT+TGG	0.545080	8.3:-15770889	MS.gene58419:CDS
GTCAAAGCGAAACATGGCTC+CGG	0.549165	8.3:-15770355	MS.gene58419:CDS
GACTCCAGCTCGAACAACTC+CGG	0.551808	8.3:+15770637	None:intergenic
TTTGGTTGAATGCAACCAAA+GGG	0.554402	8.3:+15770895	None:intergenic
TCAGGTCAGGGCCCCAATTG+CGG	0.557468	8.3:+15770602	None:intergenic
ATGTTTCAGACTCGGTGGCT+AGG	0.557537	8.3:+15771220	None:intergenic
AAGTGGGGACAACACCAGGC+TGG	0.558202	8.3:+15770965	None:intergenic
GATGATGGTGTCCGCAATTG+GGG	0.560110	8.3:-15770613	MS.gene58419:CDS
CCAGCATGAAAGACACCAGC+AGG	0.562119	8.3:+15770493	None:intergenic
GGGTTAAACCCTCTTCAGCA+AGG	0.563127	8.3:+15771247	None:intergenic
ATCTTGAGACTCCTCAGAGT+TGG	0.563156	8.3:+15770222	None:intergenic
CAGGTAGTCCAAGCACCAAA+GGG	0.564684	8.3:+15770263	None:intergenic
GAAGAAGAGAAAGAGGATGC+TGG	0.568456	8.3:-15770182	MS.gene58419:CDS
AAGGAGCGGTGACATTAATA+CGG	0.569148	8.3:+15770782	None:intergenic
CCTTCCCACCTCAATATCGC+TGG	0.569995	8.3:-15771276	MS.gene58419:CDS
ATAGGATAGTGATCAGAAGC+AGG	0.576456	8.3:+15769330	None:intergenic
AGATACACAAGGCCGTGGTG+TGG	0.576761	8.3:+15769290	None:intergenic
AACAGTGTCAAAGCGAAACA+TGG	0.576976	8.3:-15770361	MS.gene58419:CDS
GATTGTCAACAGCAAGCATA+CGG	0.578956	8.3:+15770422	None:intergenic
CCAGCGATATTGAGGTGGGA+AGG	0.579726	8.3:+15771276	None:intergenic
GCAGCGGAACCAATACGGGC+AGG	0.580756	8.3:+15770571	None:intergenic
CAGCGATATTGAGGTGGGAA+GGG	0.581963	8.3:+15771277	None:intergenic
CGGACCGTCATCAAGAATGC+GGG	0.583338	8.3:+15771026	None:intergenic
CAAGTGTATCTGACAAGCAC+CGG	0.585274	8.3:+15770336	None:intergenic
ATGGTTCAAGTCGACAGAAG+TGG	0.586579	8.3:+15770948	None:intergenic
GAGTTACCTATAGAAGAAGA+AGG	0.589211	8.3:+15770310	None:intergenic
AGGATGCTGGCCCTTCAGAA+TGG	0.591615	8.3:-15770169	MS.gene58419:CDS
TACACAAGGCCGTGGTGTGG+GGG	0.593524	8.3:+15769293	None:intergenic
ATTCAACCAAATGTTGGTCA+AGG	0.595811	8.3:-15770883	MS.gene58419:CDS
TGAAATTTCACCATTCTGAA+GGG	0.596074	8.3:+15770159	None:intergenic
CAACCATTTATTATTAGCAG+CGG	0.602480	8.3:+15770555	None:intergenic
GCTGCCCGCATTCTTGATGA+CGG	0.605798	8.3:-15771030	MS.gene58419:CDS
TGTTTAGATACACAAGGCCG+TGG	0.609248	8.3:+15769285	None:intergenic
ATTTGGTTGAATGCAACCAA+AGG	0.609384	8.3:+15770894	None:intergenic
TGGAAAGTCCGAGTTAGTAA+TGG	0.609448	8.3:-15770748	MS.gene58419:CDS
TGAGGCCAGCGATATTGAGG+TGG	0.610022	8.3:+15771271	None:intergenic
GATAGTGGATGTTTCAGACT+CGG	0.611459	8.3:+15771212	None:intergenic
GCATTCTTGATGACGGTCCG+TGG	0.613963	8.3:-15771023	MS.gene58419:CDS
GGTTAAACCCTCTTCAGCAA+GGG	0.617085	8.3:+15771248	None:intergenic
GTAATGGACCGTATCCAGCC+TGG	0.617163	8.3:-15770979	MS.gene58419:CDS
AATAGGCAGCCTGAATCTCA+CGG	0.617647	8.3:+15771154	None:intergenic
GCAGGTAGTCCAAGCACCAA+AGG	0.618179	8.3:+15770262	None:intergenic
TCATCATTTGCTGCTGATGG+GGG	0.624594	8.3:-15770139	MS.gene58419:intron
CCAGCAGGAACATAGTGGCT+CGG	0.625474	8.3:+15770508	None:intergenic
GGCCGAAACTCTCCTCCTAG+TGG	0.633512	8.3:-15770472	MS.gene58419:CDS
ACAGCATTGACAGCACCACT+AGG	0.637601	8.3:+15770457	None:intergenic
AGACACCAGCAGGAACATAG+TGG	0.640185	8.3:+15770503	None:intergenic
GAGGCCAGCGATATTGAGGT+GGG	0.647056	8.3:+15771272	None:intergenic
ACGGACCGTCATCAAGAATG+CGG	0.654680	8.3:+15771025	None:intergenic
GCATTGACAGCACCACTAGG+AGG	0.655539	8.3:+15770460	None:intergenic
GTAGTCCAAGCACCAAAGGG+CGG	0.668886	8.3:+15770266	None:intergenic
TGGAAATTATCGTACAGCCA+CGG	0.685964	8.3:+15771006	None:intergenic
GGTTCAAGTCGACAGAAGTG+GGG	0.688223	8.3:+15770950	None:intergenic
AGTGGATGTTTCAGACTCGG+TGG	0.691705	8.3:+15771215	None:intergenic
GTTTCCAATTACCAACTCTG+AGG	0.709389	8.3:-15770233	MS.gene58419:CDS
TCTCACGGTCAGCTAAGACA+CGG	0.715862	8.3:+15771169	None:intergenic
AAGCGATGTTTAGATACACA+AGG	0.719194	8.3:+15769279	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAATAATTTCAACAGTAATA+TGG	+	chr8.3:15770675-15770694	None:intergenic	20.0%
!!!	ACTTTTAAATATGAGAAACT+TGG	-	chr8.3:15769693-15769712	MS.gene58419:intron	20.0%
!!!	GAAGTTCTTCAATTTTATTT+CGG	+	chr8.3:15770537-15770556	None:intergenic	20.0%
!	AAAGTTTCAACAATAGCATT+CGG	+	chr8.3:15771045-15771064	None:intergenic	25.0%
!	CAATTATCTAACCAATTTAC+AGG	-	chr8.3:15770781-15770800	MS.gene58419:CDS	25.0%
!!!	ACTTTCCATTCTTTTTTCAA+AGG	+	chr8.3:15769642-15769661	None:intergenic	25.0%
!!!	GAAATTATTGATAGTGTTCT+TGG	-	chr8.3:15770685-15770704	MS.gene58419:CDS	25.0%
	AAACCATTAATAAGCCATGT+TGG	+	chr8.3:15770877-15770896	None:intergenic	30.0%
	ACATATTATTTGCAAGAGCA+CGG	+	chr8.3:15771008-15771027	None:intergenic	30.0%
	AGGTACTATGAATAGTGTTA+CGG	+	chr8.3:15769584-15769603	None:intergenic	30.0%
	ATAGCCTCATAATAAGATCA+TGG	+	chr8.3:15771224-15771243	None:intergenic	30.0%
	ATGAAATTTCACCATTCTGA+AGG	+	chr8.3:15770247-15770266	None:intergenic	30.0%
	ATTAGGATAGCCATAATAAC+CGG	+	chr8.3:15770725-15770744	None:intergenic	30.0%
	ATTGGTTAGATAATTGTCGA+AGG	+	chr8.3:15770777-15770796	None:intergenic	30.0%
	CAACCATTTATTATTAGCAG+CGG	+	chr8.3:15769850-15769869	None:intergenic	30.0%
	CGATAATTTCCATATCGTAA+TGG	-	chr8.3:15769407-15769426	MS.gene58419:intron	30.0%
	CTCATAATAAGATCATGGTT+GGG	+	chr8.3:15771219-15771238	None:intergenic	30.0%
	GGTGAAATTGAAACAGAAAA+CGG	+	chr8.3:15769341-15769360	None:intergenic	30.0%
	GTTTCAATTTCACCACAAAA+TGG	-	chr8.3:15769347-15769366	MS.gene58419:intron	30.0%
	TCACTTAGGAATAAGATATC+CGG	+	chr8.3:15770502-15770521	None:intergenic	30.0%
	TGAAATTTCACCATTCTGAA+GGG	+	chr8.3:15770246-15770265	None:intergenic	30.0%
	TGCTATTGTTGAAACTTTAG+TGG	-	chr8.3:15771047-15771066	MS.gene58419:CDS	30.0%
	TTGTTACCTTCTTCTTCTAT+AGG	-	chr8.3:15770086-15770105	MS.gene58419:intron	30.0%
	TTTACTTGGTTCAAAAGTCT+TGG	-	chr8.3:15770952-15770971	MS.gene58419:CDS	30.0%
!	AGACTTTTGAACCAAGTAAA+CGG	+	chr8.3:15770952-15770971	None:intergenic	30.0%
!	ATAGGAACAAATGTTAGTCT+TGG	-	chr8.3:15770302-15770321	MS.gene58419:CDS	30.0%
!	GTTTGATGACACTAATGTAA+TGG	-	chr8.3:15770361-15770380	MS.gene58419:CDS	30.0%
!!	TAGGAACAAATGTTAGTCTT+GGG	-	chr8.3:15770303-15770322	MS.gene58419:CDS	30.0%
!!!	AACTTGGAGTTTTTTGTTTC+AGG	-	chr8.3:15769709-15769728	MS.gene58419:intron	30.0%
	AAATCTCCGAAAATACACCA+GGG	+	chr8.3:15770808-15770827	None:intergenic	35.0%
	AACAAGAAGGTCATGTTTGA+GGG	+	chr8.3:15770015-15770034	None:intergenic	35.0%
	AAGCGATGTTTAGATACACA+AGG	+	chr8.3:15771126-15771145	None:intergenic	35.0%
	AATAGTGTTACGGACATCAT+AGG	+	chr8.3:15769574-15769593	None:intergenic	35.0%
	ACATTAGTGTCATCAAACAG+AGG	+	chr8.3:15770359-15770378	None:intergenic	35.0%
	ACTTGAACCATTTGGACATA+TGG	-	chr8.3:15769466-15769485	MS.gene58419:intron	35.0%
	AGACCAACATGGCTTATTAA+TGG	-	chr8.3:15770871-15770890	MS.gene58419:CDS	35.0%
	ATCCTTGATCACTGTTTGAT+TGG	-	chr8.3:15769210-15769229	MS.gene58419:intron	35.0%
	ATTCAACCAAATGTTGGTCA+AGG	-	chr8.3:15769519-15769538	MS.gene58419:intron	35.0%
	ATTTGGTTGAATGCAACCAA+AGG	+	chr8.3:15769511-15769530	None:intergenic	35.0%
	CATAAGCACCATTACTAACT+CGG	+	chr8.3:15769665-15769684	None:intergenic	35.0%
	CCAACCATGATCTTATTATG+AGG	-	chr8.3:15771217-15771236	MS.gene58419:CDS	35.0%
	CCTCATAATAAGATCATGGT+TGG	+	chr8.3:15771220-15771239	None:intergenic	35.0%
	CTGAATCAATAACAGCTTGA+CGG	+	chr8.3:15770900-15770919	None:intergenic	35.0%
	CTTGAACCATTTGGACATAT+GGG	-	chr8.3:15769467-15769486	MS.gene58419:intron	35.0%
	GAGTTACCTATAGAAGAAGA+AGG	+	chr8.3:15770095-15770114	None:intergenic	35.0%
	GGGGCAACATTAACATAAAT+AGG	+	chr8.3:15771093-15771112	None:intergenic	35.0%
	GGTTATTATGGCTATCCTAA+TGG	-	chr8.3:15770724-15770743	MS.gene58419:CDS	35.0%
	GTAACAAGTTCCTTAAAACC+TGG	+	chr8.3:15771177-15771196	None:intergenic	35.0%
	GTGCTCTTGCAAATAATATG+TGG	-	chr8.3:15771007-15771026	MS.gene58419:CDS	35.0%
	GTGTATCTAAACATCGCTTT+AGG	-	chr8.3:15771127-15771146	MS.gene58419:CDS	35.0%
	GTTCCGCTGCTAATAATAAA+TGG	-	chr8.3:15769844-15769863	MS.gene58419:intron	35.0%
	TAAACGGATAACCAACAGTT+GGG	+	chr8.3:15770936-15770955	None:intergenic	35.0%
	TATTATGGCTATCCTAATGG+AGG	-	chr8.3:15770727-15770746	MS.gene58419:CDS	35.0%
	TCTTGATGCTAGCGAAAAAA+GGG	-	chr8.3:15770837-15770856	MS.gene58419:CDS	35.0%
	TTAAGATCAGGAATTGTGCT+CGG	+	chr8.3:15770457-15770476	None:intergenic	35.0%
	TTCTTGATGCTAGCGAAAAA+AGG	-	chr8.3:15770836-15770855	MS.gene58419:CDS	35.0%
	TTTGGTTGAATGCAACCAAA+GGG	+	chr8.3:15769510-15769529	None:intergenic	35.0%
!	ACGGTCTAATTTTTCTTCGA+CGG	+	chr8.3:15770989-15771008	None:intergenic	35.0%
!	ATTTCATCATTTGCTGCTGA+TGG	-	chr8.3:15770260-15770279	MS.gene58419:CDS	35.0%
!	GCTTTAGGTATGAAAATGCA+TGG	-	chr8.3:15771142-15771161	MS.gene58419:CDS	35.0%
!	GTATCTTGAACCAGGTTTTA+AGG	-	chr8.3:15771164-15771183	MS.gene58419:CDS	35.0%
!	TTTCATCATTTGCTGCTGAT+GGG	-	chr8.3:15770261-15770280	MS.gene58419:CDS	35.0%
!!	AGGAACAAATGTTAGTCTTG+GGG	-	chr8.3:15770304-15770323	MS.gene58419:CDS	35.0%
!!	TCAGAGTTGGTAATTGGAAA+CGG	+	chr8.3:15770170-15770189	None:intergenic	35.0%
!!	TTGATAGTGTTCTTGGTGTA+TGG	-	chr8.3:15770692-15770711	MS.gene58419:CDS	35.0%
!!!	CCATTCTTTTTTCAAAGGAG+CGG	+	chr8.3:15769637-15769656	None:intergenic	35.0%
!!!	GAAGAACTTCGTTTTTTGCT+TGG	-	chr8.3:15770547-15770566	MS.gene58419:CDS	35.0%
	AACAGTGTCAAAGCGAAACA+TGG	-	chr8.3:15770041-15770060	MS.gene58419:intron	40.0%
	AAGGAGCGGTGACATTAATA+CGG	+	chr8.3:15769623-15769642	None:intergenic	40.0%
	AATGCATGGTATCTTGAACC+AGG	-	chr8.3:15771156-15771175	MS.gene58419:CDS	40.0%
	ACACACTGACAAAGTTTGTC+CGG	-	chr8.3:15769746-15769765	MS.gene58419:intron	40.0%
	ATAGGATAGTGATCAGAAGC+AGG	+	chr8.3:15771075-15771094	None:intergenic	40.0%
	CAAACAGTGATCAAGGATAG+TGG	+	chr8.3:15769208-15769227	None:intergenic	40.0%
	CAACAAGAAGGTCATGTTTG+AGG	+	chr8.3:15770016-15770035	None:intergenic	40.0%
	CTGAACTGAAGAAGAGAAAG+AGG	-	chr8.3:15770213-15770232	MS.gene58419:CDS	40.0%
	CTGATCTTAAGTACCTAGTC+CGG	-	chr8.3:15770467-15770486	MS.gene58419:CDS	40.0%
	CTTGATGCTAGCGAAAAAAG+GGG	-	chr8.3:15770838-15770857	MS.gene58419:CDS	40.0%
	GAAATCTCCGAAAATACACC+AGG	+	chr8.3:15770809-15770828	None:intergenic	40.0%
	GATGACACTAATGTAATGGC+AGG	-	chr8.3:15770365-15770384	MS.gene58419:CDS	40.0%
	GATTGTCAACAGCAAGCATA+CGG	+	chr8.3:15769983-15770002	None:intergenic	40.0%
	GGTGAGGGAAACTTTGTAAT+AGG	-	chr8.3:15770284-15770303	MS.gene58419:CDS	40.0%
	GGTTGCATTCAACCAAATGT+TGG	-	chr8.3:15769513-15769532	MS.gene58419:intron	40.0%
	GTAAACGGATAACCAACAGT+TGG	+	chr8.3:15770937-15770956	None:intergenic	40.0%
	GTTTCCAATTACCAACTCTG+AGG	-	chr8.3:15770169-15770188	MS.gene58419:CDS	40.0%
	TAAGATCAGGAATTGTGCTC+GGG	+	chr8.3:15770456-15770475	None:intergenic	40.0%
	TATTAGCAGCGGAACCAATA+CGG	+	chr8.3:15769839-15769858	None:intergenic	40.0%
	TCAGCTTCAAACTTACTTGC+TGG	-	chr8.3:15770113-15770132	MS.gene58419:intron	40.0%
	TGGAAAGTCCGAGTTAGTAA+TGG	-	chr8.3:15769654-15769673	MS.gene58419:intron	40.0%
	TGGAAATTATCGTACAGCCA+CGG	+	chr8.3:15769399-15769418	None:intergenic	40.0%
	TTGATGCTAGCGAAAAAAGG+GGG	-	chr8.3:15770839-15770858	MS.gene58419:CDS	40.0%
	TTGTCGAAGGAAATTCCAAG+CGG	+	chr8.3:15770764-15770783	None:intergenic	40.0%
!	AATAAGCCATGTTGGTCTAG+TGG	+	chr8.3:15770869-15770888	None:intergenic	40.0%
!	AATTTTTCTTCGACGGCACT+TGG	+	chr8.3:15770982-15771001	None:intergenic	40.0%
!	CCGCTCCTTTGAAAAAAGAA+TGG	-	chr8.3:15769634-15769653	MS.gene58419:intron	40.0%
!	CTGTTGGTTATCCGTTTACT+TGG	-	chr8.3:15770938-15770957	MS.gene58419:CDS	40.0%
!	TGGAGACTTACCGGTTATTA+TGG	-	chr8.3:15770712-15770731	MS.gene58419:CDS	40.0%
!	TTCATCATTTGCTGCTGATG+GGG	-	chr8.3:15770262-15770281	MS.gene58419:CDS	40.0%
!!	AACAAATGTTAGTCTTGGGG+GGG	-	chr8.3:15770307-15770326	MS.gene58419:CDS	40.0%
!!	AGGAACTTGTTACTAACTCG+TGG	-	chr8.3:15771184-15771203	MS.gene58419:CDS	40.0%
!!	CGTCAAGCTGTTATTGATTC+AGG	-	chr8.3:15770898-15770917	MS.gene58419:CDS	40.0%
!!	GAACAAATGTTAGTCTTGGG+GGG	-	chr8.3:15770306-15770325	MS.gene58419:CDS	40.0%
!!	GATAGTGGATGTTTCAGACT+CGG	+	chr8.3:15769193-15769212	None:intergenic	40.0%
!!	GGAACAAATGTTAGTCTTGG+GGG	-	chr8.3:15770305-15770324	MS.gene58419:CDS	40.0%
!!	TTCTGTCGACTTGAACCATT+TGG	-	chr8.3:15769458-15769477	MS.gene58419:intron	40.0%
!!!	GGAGTTTTTTGTTTCAGGTG+TGG	-	chr8.3:15769714-15769733	MS.gene58419:intron	40.0%
	AATAGGCAGCCTGAATCTCA+CGG	+	chr8.3:15769251-15769270	None:intergenic	45.0%
	ACGGACTTTCAGACGCATAT+AGG	+	chr8.3:15769604-15769623	None:intergenic	45.0%
	AGATATCCGGATTGAAGTGC+CGG	+	chr8.3:15770489-15770508	None:intergenic	45.0%
	AGCCATACGCTCACTGAAAT+AGG	+	chr8.3:15769268-15769287	None:intergenic	45.0%
	ATCTTGAGACTCCTCAGAGT+TGG	+	chr8.3:15770183-15770202	None:intergenic	45.0%
	ATGGTTCAAGTCGACAGAAG+TGG	+	chr8.3:15769457-15769476	None:intergenic	45.0%
	ATTAGCAGCGGAACCAATAC+GGG	+	chr8.3:15769838-15769857	None:intergenic	45.0%
	CAAGTGTATCTGACAAGCAC+CGG	+	chr8.3:15770069-15770088	None:intergenic	45.0%
	CCAAATGTTGGTCAAGGCAT+AGG	-	chr8.3:15769525-15769544	MS.gene58419:intron	45.0%
	CCTATGCCTTGACCAACATT+TGG	+	chr8.3:15769528-15769547	None:intergenic	45.0%
	CTAACCAATTTACAGGTCCC+TGG	-	chr8.3:15770788-15770807	MS.gene58419:CDS	45.0%
	CTAGACTCATTTACCTAGGC+AGG	+	chr8.3:15770405-15770424	None:intergenic	45.0%
	GAAGAAGAGAAAGAGGATGC+TGG	-	chr8.3:15770220-15770239	MS.gene58419:CDS	45.0%
	GCCACTAGACTCATTTACCT+AGG	+	chr8.3:15770409-15770428	None:intergenic	45.0%
	GCCTAGGTAAATGAGTCTAG+TGG	-	chr8.3:15770405-15770424	MS.gene58419:CDS	45.0%
	GGATACGGTCCATTACGATA+TGG	+	chr8.3:15769419-15769438	None:intergenic	45.0%
	GGCCAATCAAACAGTGATCA+AGG	+	chr8.3:15769215-15769234	None:intergenic	45.0%
	GGTAAATGAGTCTAGTGGCA+TGG	-	chr8.3:15770410-15770429	MS.gene58419:CDS	45.0%
	GGTTAAACCCTCTTCAGCAA+GGG	+	chr8.3:15769157-15769176	None:intergenic	45.0%
	GTAATTGGAAACGGTTCAGC+AGG	+	chr8.3:15770161-15770180	None:intergenic	45.0%
	GTCTAGTGGCATGGAATTGT+CGG	-	chr8.3:15770419-15770438	MS.gene58419:CDS	45.0%
	TACACCAGGGACCTGTAAAT+TGG	+	chr8.3:15770795-15770814	None:intergenic	45.0%
	TCTAGTGGCATGGAATTGTC+GGG	-	chr8.3:15770420-15770439	MS.gene58419:CDS	45.0%
	TGCCTATTTCAGTGAGCGTA+TGG	-	chr8.3:15769263-15769282	MS.gene58419:intron	45.0%
	TGGTTCAAGTCGACAGAAGT+GGG	+	chr8.3:15769456-15769475	None:intergenic	45.0%
	TGTTTAGATACACAAGGCCG+TGG	+	chr8.3:15771120-15771139	None:intergenic	45.0%
	TTGTCTGATGTCCCAACTGT+TGG	-	chr8.3:15770922-15770941	MS.gene58419:CDS	45.0%
!	ACAGGTCCCTGGTGTATTTT+CGG	-	chr8.3:15770799-15770818	MS.gene58419:CDS	45.0%
!	ACTTGTTACTAACTCGTGGC+AGG	-	chr8.3:15771188-15771207	MS.gene58419:CDS	45.0%
!	CACTGTTTTGCGCAACAAGA+AGG	+	chr8.3:15770028-15770047	None:intergenic	45.0%
!	CGGACTAGGTACTTAAGATC+AGG	+	chr8.3:15770469-15770488	None:intergenic	45.0%
!	CTTGGTGTATGGAGACTTAC+CGG	-	chr8.3:15770703-15770722	MS.gene58419:CDS	45.0%
!	TCATCATTTGCTGCTGATGG+GGG	-	chr8.3:15770263-15770282	MS.gene58419:CDS	45.0%
!!	GACTCCTCAGAGTTGGTAAT+TGG	+	chr8.3:15770176-15770195	None:intergenic	45.0%
!!	TGATGATGGTGTCCGCAATT+GGG	-	chr8.3:15769788-15769807	MS.gene58419:intron	45.0%
!!	TGTTTCAGGTGTGGTTTGCT+TGG	-	chr8.3:15769723-15769742	MS.gene58419:intron	45.0%
	AAGCCTAATCCTGCCCGTAT+TGG	-	chr8.3:15769822-15769841	MS.gene58419:intron	50.0%
	AATGGCAGGTCCTGATGATC+AGG	-	chr8.3:15770379-15770398	MS.gene58419:CDS	50.0%
	ACGGACCGTCATCAAGAATG+CGG	+	chr8.3:15769380-15769399	None:intergenic	50.0%
	AGACACCAGCAGGAACATAG+TGG	+	chr8.3:15769902-15769921	None:intergenic	50.0%
	AGTGGATGTTTCAGACTCGG+TGG	+	chr8.3:15769190-15769209	None:intergenic	50.0%
	CAAGGCATAGGCAGCTTTCT+AGG	-	chr8.3:15769537-15769556	MS.gene58419:intron	50.0%
	CACCACTAGGAGGAGAGTTT+CGG	+	chr8.3:15769935-15769954	None:intergenic	50.0%
	CAGCGATATTGAGGTGGGAA+GGG	+	chr8.3:15769128-15769147	None:intergenic	50.0%
	CAGGATTAGGCTTCAGGTCA+GGG	+	chr8.3:15769815-15769834	None:intergenic	50.0%
	CAGGTAGTCCAAGCACCAAA+GGG	+	chr8.3:15770142-15770161	None:intergenic	50.0%
	CCATTTGGACATATGGGTCC+AGG	-	chr8.3:15769473-15769492	MS.gene58419:intron	50.0%
	CCTGGACCCATATGTCCAAA+TGG	+	chr8.3:15769476-15769495	None:intergenic	50.0%
	CTAGTGGCATGGAATTGTCG+GGG	-	chr8.3:15770421-15770440	MS.gene58419:CDS	50.0%
	CTTAGCTGACCGTGAGATTC+AGG	-	chr8.3:15769239-15769258	MS.gene58419:intron	50.0%
	GAACCAATACGGGCAGGATT+AGG	+	chr8.3:15769828-15769847	None:intergenic	50.0%
	GACATATGGGTCCAGGTTCA+CGG	-	chr8.3:15769480-15769499	MS.gene58419:intron	50.0%
	GAGGAGTCTCAAGATGCTAC+TGG	-	chr8.3:15770188-15770207	MS.gene58419:CDS	50.0%
	GCATGGAATTGTCGGGGTAT+AGG	-	chr8.3:15770427-15770446	MS.gene58419:CDS	50.0%
	GCTGTTCTACGACCTCCATT+AGG	+	chr8.3:15770742-15770761	None:intergenic	50.0%
	GGGTTAAACCCTCTTCAGCA+AGG	+	chr8.3:15769158-15769177	None:intergenic	50.0%
	GGTGAGCTAGAGTCTCACTT+AGG	+	chr8.3:15770516-15770535	None:intergenic	50.0%
	GGTTCAAGTCGACAGAAGTG+GGG	+	chr8.3:15769455-15769474	None:intergenic	50.0%
	GTCAAAGCGAAACATGGCTC+CGG	-	chr8.3:15770047-15770066	MS.gene58419:intron	50.0%
	TCGGCACAGCAGTAGGAATT+GGG	+	chr8.3:15769878-15769897	None:intergenic	50.0%
	TCTCACGGTCAGCTAAGACA+CGG	+	chr8.3:15769236-15769255	None:intergenic	50.0%
	TTAATGTTGCCCCCACACCA+CGG	-	chr8.3:15771100-15771119	MS.gene58419:CDS	50.0%
	TTCGAGCTGGAGTCTGATGA+TGG	-	chr8.3:15769774-15769793	MS.gene58419:intron	50.0%
!	GATGATGGTGTCCGCAATTG+GGG	-	chr8.3:15769789-15769808	MS.gene58419:intron	50.0%
!	TGTTTTCCTGTAGACCGCAC+CGG	-	chr8.3:15770580-15770599	MS.gene58419:CDS	50.0%
!!	ACAGCATTGACAGCACCACT+AGG	+	chr8.3:15769948-15769967	None:intergenic	50.0%
!!	AGACTCGGTGGCTAGGTTAA+GGG	+	chr8.3:15769178-15769197	None:intergenic	50.0%
!!	ATGTTTCAGACTCGGTGGCT+AGG	+	chr8.3:15769185-15769204	None:intergenic	50.0%
!!	CTGATGATGGTGTCCGCAAT+TGG	-	chr8.3:15769787-15769806	MS.gene58419:intron	50.0%
!!!	GCGGGGGTCTAGCTTTATTT+TGG	-	chr8.3:15770608-15770627	MS.gene58419:CDS	50.0%
	AACCCTCTTCAGCAAGGGTG+AGG	+	chr8.3:15769152-15769171	None:intergenic	55.0%
	ACAGAAGTGGGGACAACACC+AGG	+	chr8.3:15769444-15769463	None:intergenic	55.0%
	ACGATCGAAACACGCTTGCC+TGG	+	chr8.3:15769303-15769322	None:intergenic	55.0%
	AGATACACAAGGCCGTGGTG+TGG	+	chr8.3:15771115-15771134	None:intergenic	55.0%
	AGGATGCTGGCCCTTCAGAA+TGG	-	chr8.3:15770233-15770252	MS.gene58419:CDS	55.0%
	ATACACAAGGCCGTGGTGTG+GGG	+	chr8.3:15771113-15771132	None:intergenic	55.0%
	CAAAGGGAAGACCGTGAACC+TGG	+	chr8.3:15769494-15769513	None:intergenic	55.0%
	CAGGTTCACGGTCTTCCCTT+TGG	-	chr8.3:15769492-15769511	MS.gene58419:intron	55.0%
	CCAGCAGGAACATAGTGGCT+CGG	+	chr8.3:15769897-15769916	None:intergenic	55.0%
	CCAGCATGAAAGACACCAGC+AGG	+	chr8.3:15769912-15769931	None:intergenic	55.0%
	CCAGCGATATTGAGGTGGGA+AGG	+	chr8.3:15769129-15769148	None:intergenic	55.0%
	CCTAGTCCGGCACTTCAATC+CGG	-	chr8.3:15770480-15770499	MS.gene58419:CDS	55.0%
	CCTGCTGGTGTCTTTCATGC+TGG	-	chr8.3:15769909-15769928	MS.gene58419:intron	55.0%
	CCTTCCCACCTCAATATCGC+TGG	-	chr8.3:15769126-15769145	MS.gene58419:CDS	55.0%
	CGGACCGTCATCAAGAATGC+GGG	+	chr8.3:15769379-15769398	None:intergenic	55.0%
	CTCGGCACAGCAGTAGGAAT+TGG	+	chr8.3:15769879-15769898	None:intergenic	55.0%
	GACTCCAGCTCGAACAACTC+CGG	+	chr8.3:15769768-15769787	None:intergenic	55.0%
	GAGGCCAGCGATATTGAGGT+GGG	+	chr8.3:15769133-15769152	None:intergenic	55.0%
	GATACACAAGGCCGTGGTGT+GGG	+	chr8.3:15771114-15771133	None:intergenic	55.0%
	GCAGGATTAGGCTTCAGGTC+AGG	+	chr8.3:15769816-15769835	None:intergenic	55.0%
	GCAGGTAGTCCAAGCACCAA+AGG	+	chr8.3:15770143-15770162	None:intergenic	55.0%
	GCGCAGACGACATGTCAGTT+TGG	-	chr8.3:15771250-15771269	MS.gene58419:CDS	55.0%
	GCTGCCCGCATTCTTGATGA+CGG	-	chr8.3:15769372-15769391	MS.gene58419:intron	55.0%
	GGACAACACCAGGCTGGATA+CGG	+	chr8.3:15769434-15769453	None:intergenic	55.0%
	GGCGTACCACTAGACCAACA+TGG	-	chr8.3:15770860-15770879	MS.gene58419:CDS	55.0%
	GTAGTCCAAGCACCAAAGGG+CGG	+	chr8.3:15770139-15770158	None:intergenic	55.0%
	TACGGGCAGGATTAGGCTTC+AGG	+	chr8.3:15769821-15769840	None:intergenic	55.0%
	TGAGGCCAGCGATATTGAGG+TGG	+	chr8.3:15769134-15769153	None:intergenic	55.0%
	TTGTCCGGAGTTGTTCGAGC+TGG	-	chr8.3:15769761-15769780	MS.gene58419:intron	55.0%
	TTTGCTGCTGATGGGGGTGA+GGG	-	chr8.3:15770269-15770288	MS.gene58419:CDS	55.0%
!	ATTTGCTGCTGATGGGGGTG+AGG	-	chr8.3:15770268-15770287	MS.gene58419:CDS	55.0%
!	GCATTGACAGCACCACTAGG+AGG	+	chr8.3:15769945-15769964	None:intergenic	55.0%
!	TGCGGGCAGCATCCATTTTG+TGG	+	chr8.3:15769362-15769381	None:intergenic	55.0%
!!	CAGACTCGGTGGCTAGGTTA+AGG	+	chr8.3:15769179-15769198	None:intergenic	55.0%
!!	GCATTCTTGATGACGGTCCG+TGG	-	chr8.3:15769379-15769398	MS.gene58419:intron	55.0%
!!	GTAATGGACCGTATCCAGCC+TGG	-	chr8.3:15769423-15769442	MS.gene58419:intron	55.0%
	AAGTGGGGACAACACCAGGC+TGG	+	chr8.3:15769440-15769459	None:intergenic	60.0%
	ATGGCTCGTGCTTGGAAGCC+AGG	-	chr8.3:15769282-15769301	MS.gene58419:CDS	60.0%
	CCGAGCCACTATGTTCCTGC+TGG	-	chr8.3:15769894-15769913	MS.gene58419:intron	60.0%
	CCTAGGCAGGCCTGATCATC+AGG	+	chr8.3:15770392-15770411	None:intergenic	60.0%
	CCTGATGATCAGGCCTGCCT+AGG	-	chr8.3:15770389-15770408	MS.gene58419:CDS	60.0%
	GAGGTCGTAGAACAGCCGCT+TGG	-	chr8.3:15770746-15770765	MS.gene58419:CDS	60.0%
	GGCCGAAACTCTCCTCCTAG+TGG	-	chr8.3:15769930-15769949	MS.gene58419:intron	60.0%
	GGCCTCACCCTTGCTGAAGA+GGG	-	chr8.3:15769147-15769166	MS.gene58419:CDS	60.0%
	GGGTGAGGCCAGCGATATTG+AGG	+	chr8.3:15769137-15769156	None:intergenic	60.0%
	GTGAGCGTATGGCTCGTGCT+TGG	-	chr8.3:15769274-15769293	MS.gene58419:intron	60.0%
	TACACAAGGCCGTGGTGTGG+GGG	+	chr8.3:15771112-15771131	None:intergenic	60.0%
	TCAGGTCAGGGCCCCAATTG+CGG	+	chr8.3:15769803-15769822	None:intergenic	60.0%
	TGGCCTCACCCTTGCTGAAG+AGG	-	chr8.3:15769146-15769165	MS.gene58419:CDS	60.0%
!	CTTACTTGCTGGCCGCCCTT+TGG	-	chr8.3:15770124-15770143	MS.gene58419:intron	60.0%
!	TAGTGGCTCGGCACAGCAGT+AGG	+	chr8.3:15769885-15769904	None:intergenic	60.0%
!!	CCGGATTGAAGTGCCGGACT+AGG	+	chr8.3:15770483-15770502	None:intergenic	60.0%
	AGCTAGACCCCCGCCTCTAC+CGG	+	chr8.3:15770602-15770621	None:intergenic	65.0%
	CCTCTACCGGTGCGGTCTAC+AGG	+	chr8.3:15770589-15770608	None:intergenic	65.0%
	GCAGCGGAACCAATACGGGC+AGG	+	chr8.3:15769834-15769853	None:intergenic	65.0%
!	CCTGTAGACCGCACCGGTAG+AGG	-	chr8.3:15770586-15770605	MS.gene58419:CDS	65.0%
!	GTAGACCGCACCGGTAGAGG+CGG	-	chr8.3:15770589-15770608	MS.gene58419:CDS	65.0%
!	TAGACCGCACCGGTAGAGGC+GGG	-	chr8.3:15770590-15770609	MS.gene58419:CDS	65.0%
!	AGACCGCACCGGTAGAGGCG+GGG	-	chr8.3:15770591-15770610	MS.gene58419:CDS	70.0%
!!	GCTGGCCGCCCTTTGGTGCT+TGG	-	chr8.3:15770131-15770150	MS.gene58419:intron	70.0%
	GACCCCCGCCTCTACCGGTG+CGG	+	chr8.3:15770597-15770616	None:intergenic	75.0%
!	GACCGCACCGGTAGAGGCGG+GGG	-	chr8.3:15770592-15770611	MS.gene58419:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	15769120	15771304	15769120	ID=MS.gene58419
chr8.3	mRNA	15769120	15771304	15769120	ID=MS.gene58419.t1;Parent=MS.gene58419
chr8.3	exon	15770140	15771304	15770140	ID=MS.gene58419.t1.exon1;Parent=MS.gene58419.t1
chr8.3	CDS	15770140	15771304	15770140	ID=cds.MS.gene58419.t1;Parent=MS.gene58419.t1
chr8.3	exon	15769276	15769357	15769276	ID=MS.gene58419.t1.exon2;Parent=MS.gene58419.t1
chr8.3	CDS	15769276	15769357	15769276	ID=cds.MS.gene58419.t1;Parent=MS.gene58419.t1
chr8.3	exon	15769120	15769168	15769120	ID=MS.gene58419.t1.exon3;Parent=MS.gene58419.t1
chr8.3	CDS	15769120	15769168	15769120	ID=cds.MS.gene58419.t1;Parent=MS.gene58419.t1

Gene Sequence

>MS.gene58419

ATGGACCCTTCCCACCTCAATATCGCTGGCCTCACCCTTGCTGAAGAGGGTTTAACCCTTAACCTAGCCACCGAGTCTGAAACATCCACTATCCTTGATCACTGTTTGATTGGCCGTGTCTTAGCTGACCGTGAGATTCAGGCTGCCTATTTCAGTGAGCGTATGGCTCGTGCTTGGAAGCCAGGCAAGCGTGTTTCGATCGTCAAAACTGATAGTGACCGTTTTCTGTTTCAATTTCACCACAAAATGGATGCTGCCCGCATTCTTGATGACGGTCCGTGGCTGTACGATAATTTCCATATCGTAATGGACCGTATCCAGCCTGGTGTTGTCCCCACTTCTGTCGACTTGAACCATTTGGACATATGGGTCCAGGTTCACGGTCTTCCCTTTGGTTGCATTCAACCAAATGTTGGTCAAGGCATAGGCAGCTTTCTAGGTGAACTCAAATCCTATGATGTCCGTAACACTATTCATAGTACCTATATGCGTCTGAAAGTCCGTATTAATGTCACCGCTCCTTTGAAAAAAGAATGGAAAGTCCGAGTTAGTAATGGTGCTTATGTTACAGTAACTTTTAAATATGAGAAACTTGGAGTTTTTTGTTTCAGGTGTGGTTTGCTTGGACACACTGACAAAGTTTGTCCGGAGTTGTTCGAGCTGGAGTCTGATGATGGTGTCCGCAATTGGGGCCCTGACCTGAAGCCTAATCCTGCCCGTATTGGTTCCGCTGCTAATAATAAATGGTTGCAAGACCCAATTCCTACTGCTGTGCCGAGCCACTATGTTCCTGCTGGTGTCTTTCATGCTGGCCGAAACTCTCCTCCTAGTGGTGCTGTCAATGCTGTAACTTTCAGTGACCGTATGCTTGCTGTTGACAATCAGATTTCTGCCCTCAAACATGACCTTCTTGTTGCGCAAAACAGTGTCAAAGCGAAACATGGCTCCGGTGCTTGTCAGATACACTTGTTACCTTCTTCTTCTATAGGTAACTCAGCTTCAAACTTACTTGCTGGCCGCCCTTTGGTGCTTGGACTACCTGCTGAACCGTTTCCAATTACCAACTCTGAGGAGTCTCAAGATGCTACTGGTTCTGAACTGAAGAAGAGAAAGAGGATGCTGGCCCTTCAGAATGGTGAAATTTCATCATTTGCTGCTGATGGGGGTGAGGGAAACTTTGTAATAGGAACAAATGTTAGTCTTGGGGGGGATGTTGATATGAATGTAATCTCTAATCCTCTGTTTGATGACACTAATGTAATGGCAGGTCCTGATGATCAGGCCTGCCTAGGTAAATGAGTCTAGTGGCATGGAATTGTCGGGGTATAGGTAGCCCGAGCACAATTCCTGATCTTAAGTACCTAGTCCGGCACTTCAATCCGGATATCTTATTCCTAAGTGAGACTCTAGCTCACCGAAATAAAATTGAAGAACTTCGTTTTTTGCTTGGTTATGACTGTTGTTTTCCTGTAGACCGCACCGGTAGAGGCGGGGGTCTAGCTTTATTTTGGCGTAACTCTTTACATTGCAAACTTGTTGATTTTTCTAGCAACCATATTACTGTTGAAATTATTGATAGTGTTCTTGGTGTATGGAGACTTACCGGTTATTATGGCTATCCTAATGGAGGTCGTAGAACAGCCGCTTGGAATTTCCTTCGACAATTATCTAACCAATTTACAGGTCCCTGGTGTATTTTCGGAGATTTCAACGATATTCTTGATGCTAGCGAAAAAAGGGGGCGTACCACTAGACCAACATGGCTTATTAATGGTTTCCGTCAAGCTGTTATTGATTCAGGTTTGTCTGATGTCCCAACTGTTGGTTATCCGTTTACTTGGTTCAAAAGTCTTGGTACTCCAAGTGCCGTCGAAGAAAAATTAGACCGTGCTCTTGCAAATAATATGTGGTTTAATATTTTTCCGAATGCTATTGTTGAAACTTTAGTGGCACCTGCTTCTGATCACTATCCTATTTATGTTAATGTTGCCCCCACACCACGGCCTTGTGTATCTAAACATCGCTTTAGGTATGAAAATGCATGGTATCTTGAACCAGGTTTTAAGGAACTTGTTACTAACTCGTGGCAGGAAAATCCCAACCATGATCTTATTATGAGGCTATCTGTATGCGCAGACGACATGTCAGTTTGGAGAAAATCTCATTGTCACAAACTAAAAATTGA

Protein sequence

>MS.gene58419.t1

MDPSHLNIAGLTLAEEGLTLNLATESETSTILDHCLIGRVLADREIQAAYFSERMARAWKPGKRVSIVKTDSDRFLFQFHHKMDAARILDDGPWLYDNFHIVMDRIQPGVVPTSVDLNHLDIWVQVHGLPFGCIQPNVGQGIGSFLGELKSYDVRNTIHSTYMRLKVRINVTAPLKKEWKVRVSNGAYVTVTFKYEKLGVFCFRCGLLGHTDKVCPELFELESDDGVRNWGPDLKPNPARIGSAANNKWLQDPIPTAVPSHYVPAGVFHAGRNSPPSGAVNAVTFSDRMLAVDNQISALKHDLLVAQNSVKAKHGSGACQIHLLPSSSIGNSASNLLAGRPLVLGLPAEPFPITNSEESQDATGSELKKRKRMLALQNGEISSFAADGVAPASDHYPIYVNVAPTPRPCVSKHRFRRHVSLEKISLSQTKN