AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063214


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063214.t1 MTR_6g053230 53.261 184 50 1 1 184 22 169 2.74e-63 204
MS.gene063214.t1 MTR_6g033215 36.898 187 117 1 5 191 103 288 1.70e-46 155
MS.gene063214.t1 MTR_3g024240 57.391 115 45 2 80 191 55 168 5.13e-38 130
MS.gene063214.t1 MTR_0010s0370 36.364 154 98 0 22 175 24 177 7.45e-30 109
MS.gene063214.t1 MTR_0010s0350 36.364 154 98 0 22 175 24 177 7.45e-30 109
MS.gene063214.t1 MTR_3g053920 35.252 139 86 2 1 135 1 139 4.29e-24 96.7
MS.gene063214.t1 MTR_2g061500 35.115 131 81 2 1 127 1 131 2.52e-20 83.2
MS.gene063214.t1 MTR_8g468010 50.820 61 30 0 116 176 4 64 2.39e-17 75.5
MS.gene063214.t1 MTR_4g103530 37.079 89 56 0 55 143 5 93 1.72e-15 72.0
MS.gene063214.t1 MTR_1g032270 36.486 74 47 0 96 169 1 74 2.97e-13 64.7
MS.gene063214.t1 MTR_1g036720 41.429 70 41 0 30 99 32 101 3.62e-12 60.8
MS.gene063214.t1 MTR_4g020545 30.000 140 76 3 36 175 60 177 1.34e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTTGGCGACCTGGTC+AGG 0.261092 1.1:+26329024 MS.gene063214:CDS
ATTCATGATAGAACTGAAAA+TGG 0.268232 1.1:-26329081 None:intergenic
TTGCTACATCTGTGGCATAA+TGG 0.300136 1.1:+26329464 MS.gene063214:CDS
GAGTTCTACAAAACGGTCTT+TGG 0.303204 1.1:+26329116 MS.gene063214:CDS
AGGAAAGATTACCATCAATT+TGG 0.319616 1.1:+26329011 MS.gene063214:CDS
GCCTATCCGCTTCCAGTTCA+TGG 0.339997 1.1:+26328988 MS.gene063214:CDS
TCACCAAATGGATCCTCAAC+TGG 0.358593 1.1:+26328899 MS.gene063214:CDS
CTCCCAACCTTTCGTATTGA+AGG 0.368558 1.1:-26329437 None:intergenic
CTGCTTGAACTCTGTGTTGT+AGG 0.380766 1.1:+26328947 MS.gene063214:CDS
TCTTCAATGAAGTTGAAGTC+TGG 0.398848 1.1:+26329203 MS.gene063214:CDS
GTAATCTTTCCTCCATGAAC+TGG 0.423372 1.1:-26329000 None:intergenic
AATCTCAATGGTTCACCAAA+TGG 0.424442 1.1:+26328887 MS.gene063214:CDS
AACACCATGCTTCGCTTCAA+CGG 0.441845 1.1:-26329382 None:intergenic
TGACCAGGTCGCCAAATTGA+TGG 0.454031 1.1:-26329022 None:intergenic
GGCGAATGTATATGAGGATA+CGG 0.455417 1.1:+26329338 MS.gene063214:CDS
TCACTCAAGCTGGCGAGAAC+CGG 0.461738 1.1:+26329056 MS.gene063214:CDS
TCATGGGGTGTCATATTGGC+AGG 0.466283 1.1:+26329278 MS.gene063214:CDS
ATGCTCATGGGGTGTCATAT+TGG 0.467587 1.1:+26329274 MS.gene063214:CDS
GTTCTATCATGAATGGGACT+TGG 0.469626 1.1:+26329090 MS.gene063214:CDS
AATTTCAATGTCAATCTCAA+TGG 0.474080 1.1:+26328875 MS.gene063214:CDS
GACCCAGTTGAGGATCCATT+TGG 0.474914 1.1:-26328902 None:intergenic
CTCCTTCAATACGAAAGGTT+GGG 0.493104 1.1:+26329435 MS.gene063214:CDS
GATAGAACTGAAAATGGAAC+CGG 0.494769 1.1:-26329075 None:intergenic
CTTCAATGAAGTTGAAGTCT+GGG 0.501007 1.1:+26329204 MS.gene063214:CDS
TCAATTTGGCGACCTGGTCA+GGG 0.502279 1.1:+26329025 MS.gene063214:CDS
TGAAGTTGAAGTCTGGGCTC+AGG 0.504456 1.1:+26329210 MS.gene063214:CDS
GTCCTTGGCGAATGTATATG+AGG 0.506337 1.1:+26329332 MS.gene063214:CDS
TCTCCTTCAATACGAAAGGT+TGG 0.521285 1.1:+26329434 MS.gene063214:CDS
GTCTTTAAGAAGCTAGAGAT+AGG 0.525612 1.1:+26329160 MS.gene063214:CDS
AAGCATGGTGTTCGAGAAAG+AGG 0.536490 1.1:+26329393 MS.gene063214:CDS
CGAGTATGTTGGTATGCTCA+TGG 0.536633 1.1:+26329261 MS.gene063214:CDS
TTACCATCAATTTGGCGACC+TGG 0.542386 1.1:+26329019 MS.gene063214:CDS
TTTCCTCCATGAACTGGAAG+CGG 0.548484 1.1:-26328994 None:intergenic
CATGTCTCCTTCAATACGAA+AGG 0.551329 1.1:+26329430 MS.gene063214:CDS
CACCAAATGGATCCTCAACT+GGG 0.554114 1.1:+26328900 MS.gene063214:CDS
GAGTATGTTGGTATGCTCAT+GGG 0.560183 1.1:+26329262 MS.gene063214:CDS
CATACTCGTTCATGTAACCA+TGG 0.575372 1.1:-26329246 None:intergenic
CAATTTGGCGACCTGGTCAG+GGG 0.575886 1.1:+26329026 MS.gene063214:CDS
GCGAATGTATATGAGGATAC+GGG 0.576922 1.1:+26329339 MS.gene063214:CDS
TTGGAAAGAGTTCTACAAAA+CGG 0.579627 1.1:+26329109 MS.gene063214:CDS
TCCATGAACTGGAAGCGGAT+AGG 0.591288 1.1:-26328989 None:intergenic
ATACTCGTTCATGTAACCAT+GGG 0.591871 1.1:-26329245 None:intergenic
GTTCGAGAAAGAGGATGGTA+CGG 0.602893 1.1:+26329402 MS.gene063214:CDS
GGGGTTACTATCACTCAAGC+TGG 0.603702 1.1:+26329046 MS.gene063214:CDS
TATTTCAGAGGACCCAGTTG+AGG 0.610000 1.1:-26328912 None:intergenic
TGCTTCATGTGATATTTCAG+AGG 0.614016 1.1:-26328924 None:intergenic
ATGGTGTTCGAGAAAGAGGA+TGG 0.629171 1.1:+26329397 MS.gene063214:CDS
GGTTACATGAACGAGTATGT+TGG 0.643295 1.1:+26329250 MS.gene063214:CDS
ATCCTCATATACATTCGCCA+AGG 0.654983 1.1:-26329334 None:intergenic
AGGCGAGGCTATCTCTGAGG+TGG 0.655236 1.1:+26329180 MS.gene063214:CDS
AATTTGGCGACCTGGTCAGG+GGG 0.672524 1.1:+26329027 MS.gene063214:CDS
AGTATGTTGGTATGCTCATG+GGG 0.677827 1.1:+26329263 MS.gene063214:CDS
AATGTATATGAGGATACGGG+TGG 0.688504 1.1:+26329342 MS.gene063214:CDS
TGAACCGTTGAAGCGAAGCA+TGG 0.703453 1.1:+26329378 MS.gene063214:CDS
TAAGAAGCTAGAGATAGGCG+AGG 0.711724 1.1:+26329165 MS.gene063214:CDS
GATAGGCGAGGCTATCTCTG+AGG 0.715135 1.1:+26329177 MS.gene063214:CDS
TACTCGTTCATGTAACCATG+GGG 0.727613 1.1:-26329244 None:intergenic
TATCCGCTTCCAGTTCATGG+AGG 0.761704 1.1:+26328991 MS.gene063214:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATGAAAGCAATTTTTA+TGG + chr1.1:26329310-26329329 MS.gene063214:CDS 20.0%
! AATTTCAATGTCAATCTCAA+TGG + chr1.1:26328875-26328894 MS.gene063214:CDS 25.0%
! ATTCATGATAGAACTGAAAA+TGG - chr1.1:26329084-26329103 None:intergenic 25.0%
!! ATTTTCAGTTCTATCATGAA+TGG + chr1.1:26329083-26329102 MS.gene063214:CDS 25.0%
!! TTTTCAGTTCTATCATGAAT+GGG + chr1.1:26329084-26329103 MS.gene063214:CDS 25.0%
AGGAAAGATTACCATCAATT+TGG + chr1.1:26329011-26329030 MS.gene063214:CDS 30.0%
TTGGAAAGAGTTCTACAAAA+CGG + chr1.1:26329109-26329128 MS.gene063214:CDS 30.0%
! AAAGCAATTTTTATGGTCCT+TGG + chr1.1:26329317-26329336 MS.gene063214:CDS 30.0%
AATCTCAATGGTTCACCAAA+TGG + chr1.1:26328887-26328906 MS.gene063214:CDS 35.0%
ATACTCGTTCATGTAACCAT+GGG - chr1.1:26329248-26329267 None:intergenic 35.0%
CTTCAATGAAGTTGAAGTCT+GGG + chr1.1:26329204-26329223 MS.gene063214:CDS 35.0%
GATAGAACTGAAAATGGAAC+CGG - chr1.1:26329078-26329097 None:intergenic 35.0%
TCTTCAATGAAGTTGAAGTC+TGG + chr1.1:26329203-26329222 MS.gene063214:CDS 35.0%
TGCTTCATGTGATATTTCAG+AGG - chr1.1:26328927-26328946 None:intergenic 35.0%
! GTCTTTAAGAAGCTAGAGAT+AGG + chr1.1:26329160-26329179 MS.gene063214:CDS 35.0%
!! GACTTTTGATTGGATTTTGC+TGG + chr1.1:26329138-26329157 MS.gene063214:CDS 35.0%
AATGTATATGAGGATACGGG+TGG + chr1.1:26329342-26329361 MS.gene063214:CDS 40.0%
AGTATGTTGGTATGCTCATG+GGG + chr1.1:26329263-26329282 MS.gene063214:CDS 40.0%
ATCCTCATATACATTCGCCA+AGG - chr1.1:26329337-26329356 None:intergenic 40.0%
CATACTCGTTCATGTAACCA+TGG - chr1.1:26329249-26329268 None:intergenic 40.0%
CATGTCTCCTTCAATACGAA+AGG + chr1.1:26329430-26329449 MS.gene063214:CDS 40.0%
CTCCTTCAATACGAAAGGTT+GGG + chr1.1:26329435-26329454 MS.gene063214:CDS 40.0%
GAGTATGTTGGTATGCTCAT+GGG + chr1.1:26329262-26329281 MS.gene063214:CDS 40.0%
GAGTTCTACAAAACGGTCTT+TGG + chr1.1:26329116-26329135 MS.gene063214:CDS 40.0%
GCGAATGTATATGAGGATAC+GGG + chr1.1:26329339-26329358 MS.gene063214:CDS 40.0%
GGCGAATGTATATGAGGATA+CGG + chr1.1:26329338-26329357 MS.gene063214:CDS 40.0%
GGTTACATGAACGAGTATGT+TGG + chr1.1:26329250-26329269 MS.gene063214:CDS 40.0%
GTAATCTTTCCTCCATGAAC+TGG - chr1.1:26329003-26329022 None:intergenic 40.0%
GTTCTATCATGAATGGGACT+TGG + chr1.1:26329090-26329109 MS.gene063214:CDS 40.0%
TACTCGTTCATGTAACCATG+GGG - chr1.1:26329247-26329266 None:intergenic 40.0%
TCTCCTTCAATACGAAAGGT+TGG + chr1.1:26329434-26329453 MS.gene063214:CDS 40.0%
! ACGGTCTTTGGACTTTTGAT+TGG + chr1.1:26329128-26329147 MS.gene063214:CDS 40.0%
! CAGGTTTTTAATCTTCCCCA+TGG + chr1.1:26329229-26329248 MS.gene063214:CDS 40.0%
!! GGAGTATTTTGCTACATCTG+TGG + chr1.1:26329456-26329475 MS.gene063214:CDS 40.0%
AACACCATGCTTCGCTTCAA+CGG - chr1.1:26329385-26329404 None:intergenic 45.0%
AAGCATGGTGTTCGAGAAAG+AGG + chr1.1:26329393-26329412 MS.gene063214:CDS 45.0%
ATGGTGTTCGAGAAAGAGGA+TGG + chr1.1:26329397-26329416 MS.gene063214:CDS 45.0%
CACCAAATGGATCCTCAACT+GGG + chr1.1:26328900-26328919 MS.gene063214:CDS 45.0%
CGAGTATGTTGGTATGCTCA+TGG + chr1.1:26329261-26329280 MS.gene063214:CDS 45.0%
CTCCCAACCTTTCGTATTGA+AGG - chr1.1:26329440-26329459 None:intergenic 45.0%
CTGCTTGAACTCTGTGTTGT+AGG + chr1.1:26328947-26328966 MS.gene063214:CDS 45.0%
GTCCTTGGCGAATGTATATG+AGG + chr1.1:26329332-26329351 MS.gene063214:CDS 45.0%
GTTCGAGAAAGAGGATGGTA+CGG + chr1.1:26329402-26329421 MS.gene063214:CDS 45.0%
TAAGAAGCTAGAGATAGGCG+AGG + chr1.1:26329165-26329184 MS.gene063214:CDS 45.0%
TATTTCAGAGGACCCAGTTG+AGG - chr1.1:26328915-26328934 None:intergenic 45.0%
TCACCAAATGGATCCTCAAC+TGG + chr1.1:26328899-26328918 MS.gene063214:CDS 45.0%
TTACCATCAATTTGGCGACC+TGG + chr1.1:26329019-26329038 MS.gene063214:CDS 45.0%
TTTCCTCCATGAACTGGAAG+CGG - chr1.1:26328997-26329016 None:intergenic 45.0%
! ATGCTCATGGGGTGTCATAT+TGG + chr1.1:26329274-26329293 MS.gene063214:CDS 45.0%
ATCAATTTGGCGACCTGGTC+AGG + chr1.1:26329024-26329043 MS.gene063214:CDS 50.0%
GACCCAGTTGAGGATCCATT+TGG - chr1.1:26328905-26328924 None:intergenic 50.0%
GGGGTTACTATCACTCAAGC+TGG + chr1.1:26329046-26329065 MS.gene063214:CDS 50.0%
TATCCGCTTCCAGTTCATGG+AGG + chr1.1:26328991-26329010 MS.gene063214:CDS 50.0%
TCAATTTGGCGACCTGGTCA+GGG + chr1.1:26329025-26329044 MS.gene063214:CDS 50.0%
TCCATGAACTGGAAGCGGAT+AGG - chr1.1:26328992-26329011 None:intergenic 50.0%
TGAACCGTTGAAGCGAAGCA+TGG + chr1.1:26329378-26329397 MS.gene063214:CDS 50.0%
TGAAGTTGAAGTCTGGGCTC+AGG + chr1.1:26329210-26329229 MS.gene063214:CDS 50.0%
TGACCAGGTCGCCAAATTGA+TGG - chr1.1:26329025-26329044 None:intergenic 50.0%
! TCATGGGGTGTCATATTGGC+AGG + chr1.1:26329278-26329297 MS.gene063214:CDS 50.0%
AATTTGGCGACCTGGTCAGG+GGG + chr1.1:26329027-26329046 MS.gene063214:CDS 55.0%
CAATTTGGCGACCTGGTCAG+GGG + chr1.1:26329026-26329045 MS.gene063214:CDS 55.0%
GATAGGCGAGGCTATCTCTG+AGG + chr1.1:26329177-26329196 MS.gene063214:CDS 55.0%
GCCTATCCGCTTCCAGTTCA+TGG + chr1.1:26328988-26329007 MS.gene063214:CDS 55.0%
GTGATAGTAACCCCCTGACC+AGG - chr1.1:26329040-26329059 None:intergenic 55.0%
TCACTCAAGCTGGCGAGAAC+CGG + chr1.1:26329056-26329075 MS.gene063214:CDS 55.0%
! AGGCGAGGCTATCTCTGAGG+TGG + chr1.1:26329180-26329199 MS.gene063214:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 26328869 26329483 26328869 ID=MS.gene063214
chr1.1 mRNA 26328869 26329483 26328869 ID=MS.gene063214.t1;Parent=MS.gene063214
chr1.1 exon 26328869 26329483 26328869 ID=MS.gene063214.t1.exon1;Parent=MS.gene063214.t1
chr1.1 CDS 26328869 26329483 26328869 ID=cds.MS.gene063214.t1;Parent=MS.gene063214.t1
Gene Sequence

>MS.gene063214

ATGGAAAATTTCAATGTCAATCTCAATGGTTCACCAAATGGATCCTCAACTGGGTCCTCTGAAATATCACATGAAGCACTGCTTGAACTCTGTGTTGTAGGCACAGTGCTCACTGACAAGCCTATCCGCTTCCAGTTCATGGAGGAAAGATTACCATCAATTTGGCGACCTGGTCAGGGGGTTACTATCACTCAAGCTGGCGAGAACCGGTTCCATTTTCAGTTCTATCATGAATGGGACTTGGAAAGAGTTCTACAAAACGGTCTTTGGACTTTTGATTGGATTTTGCTGGTCTTTAAGAAGCTAGAGATAGGCGAGGCTATCTCTGAGGTGGTCTTCAATGAAGTTGAAGTCTGGGCTCAGGTTTTTAATCTTCCCCATGGTTACATGAACGAGTATGTTGGTATGCTCATGGGGTGTCATATTGGCAGGTTTGTTCATTTTGATGAAAGCAATTTTTATGGTCCTTGGCGAATGTATATGAGGATACGGGTGGCTCTCATCATTGCTGAACCGTTGAAGCGAAGCATGGTGTTCGAGAAAGAGGATGGTACGGCTGTTCATGTCTCCTTCAATACGAAAGGTTGGGAGTATTTTGCTACATCTGTGGCATAA

Protein sequence

>MS.gene063214.t1

MENFNVNLNGSPNGSSTGSSEISHEALLELCVVGTVLTDKPIRFQFMEERLPSIWRPGQGVTITQAGENRFHFQFYHEWDLERVLQNGLWTFDWILLVFKKLEIGEAISEVVFNEVEVWAQVFNLPHGYMNEYVGMLMGCHIGRFVHFDESNFYGPWRMYMRIRVALIIAEPLKRSMVFEKEDGTAVHVSFNTKGWEYFATSVA